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  • unnatural amino
  • The development of methods to encode unnatural amino acids (UAAs) with novel properties genetically in living cells has significantly enhanced our ability to manipulate protein structure and function ( 1 ). (pnas.org)
  • PNAS
  • described a trans-acting DNA-based amphiphatic delivery system for convenient delivery of poly A tailed uncharged nucleic acids (UNA) such as PNAs and morpholinos, so that several UNA's can be easily screened ex vivo. (wikipedia.org)
  • suppress
  • To use a heterologous tRNA/aaRS pair to encode a noncanonical amino acid, the pair must be recoded to suppress a nonsense or frameshift codon, and the recoding necessitates mutating the anticodon of the corresponding tRNA. (pnas.org)
  • Nucleotides
  • This brief review provides a hypothesis based on the absence of nucleotides and enzymes under prebiotic conditions and on the polymerization of ribose 5-phosphate units leading to the polarized formation of the ribose-phosphate backbone. (qxmd.com)
  • Both DNA and RNA are nucleic acids , which use base pairs of nucleotides as a complementary language. (ipfs.io)
  • As with natural nucleic acids, pairing between artificial nucleotides can be influenced by tautomerism, with different placements of protons on the heterocyclic nucleobase changing patterns of hydrogen bonding that determine replication fidelity. (ffame.org)
  • oligonucleotides
  • Recent examples synthesized in our group include arabinonucleic acids (e.g., 2'-fluoro-ANA (FANA) and ANA), 2',5'-linked RNA, 2',4'-difluoro-RNA, and oxepane nucleic acids (oligonucleotides with a 7-membered carbohydrate ring). (mcgill.ca)
  • The exceptional ability of aptamers to bind to these non-nucleic acid targets is due to the secondary structures (e.g. hairpins, loops, quadruplexes) that oligonucleotides can adopt, rather than Watson-Crick hydrogen bonding or base stacking. (atdbio.com)
  • interactions
  • More recently, our group reported the in situ synthesis of RNA on microarrays for the study and discovery of protein-RNA interactions that are relevant to important biological processes (e.g. (mcgill.ca)
  • Interactions between DNA and RNA and other nucleic acids are exploited by Nature, for example in transcription and DNA replication, and in biotechnology in PCR. (atdbio.com)
  • Interactions between the intermediates that affect the production of terpenes could be observed through the introduction of inhibitors that established feedback loops within and crosstalk between the pathways. (biomedcentral.com)
  • ribose
  • A chemoenzymatic synthesis of 7dSh using S. elongatus transketolase as catalyst and 5-deoxy-D-ribose as substrate allows antimicrobial and herbicidal bioprofiling. (qxmd.com)
  • The steric relationship of the C2- and C3-OH groups favored the selection of ribose, ribonucleotide, and RNA synthesis and excluded the possibility of xenonucleic acid-based life on Earth. (qxmd.com)
  • protein
  • The order of the amino acids that make up a given protein is determined by the DNA code. (massgeneral.org)
  • It sounds complicated to name all the amino acids, but if you think of each as a just a letter in the chain of a protein, it makes it easier to think about. (massgeneral.org)
  • Structurally, ribonuclease T 1 is a small α+β protein (104 amino acids ) with a four-stranded, antiparallel beta sheet covering a long alpha helix (almost five turns). (wikivisually.com)
  • A DNA transcription unit encoding for a protein may contain both a coding sequence , which will be translated into the protein, and regulatory sequences , which direct and regulate the synthesis of that protein. (ipfs.io)
  • Finally, the binding mechanism of the alpha-helical 37 amino acid residue winter flounder antifreeze protein, which is a candidate as a kinetic hydrate inhibitor to methane hydrate, is determined to be the result of cooperative anchoring of the pendant methyl groups of the threonine and two alanine residues, four and seven places further down in the protein sequence, to the empty half cages at the hydrate surface. (ubc.ca)
  • siRNA
  • We are particularly interested in developing new methods for the synthesis of nucleoside building blocks and RNA, including modified siRNA/miRNA, branched RNA and lariat RNA. (mcgill.ca)
  • ligands
  • The results demonstrate that modification of the oxide ligands on silica via the creation of Al Lewis acid center as an anchoring site for organometallic complexes opens up new catalytic properties, markedly enhancing the catalytic performance of supported organo-tungsten species. (edu.sa)
  • Receptors typically dissociate from ligands in these acidic compartments, which allows the free receptor to cycle back to the cell surface, whereas ligands are often degraded on delivery to lysosomes, which liberates amino acids and other nutrients. (schoolbag.info)
  • metabolites
  • Targeted chromatography/mass spectrometry analysis was performed in 24 h urine samples for 47 amino metabolites and 10 metabolites related to the tricarboxylic acid cycle. (creative-peptides.com)
  • strand
  • First strand cDNA is synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. The remaining overhangs are converted into blunt ends using exonuclease/polymerase activity. (rqswkl.com)
  • molecular
  • In their 1953 "Molecular Structure Of Nucleic Acids" Watson and Crick open with the decidedly unscientific "We wish to suggest…" and in only a short page or so, take us through their thoughts on what shape DNA takes in the natural world. (gillespielab.org)
  • flexibility
  • The complex [Gd(L)(H2O)](3-) (H6L = N,N-'-bis(6-carboxy-2-pyridylmethyl) ethylenediamine-N,N'-methylenephosphonic acid) displays the highest water exchange rate ever measured for a Gd(III) chelate (k(ex)(298) = 8.8 x 10(8) s(-1)), which is attributed to the flexibility of the metal coordination environment. (cnrs-orleans.fr)
  • atoms
  • DOTA (1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid) contains four acetic acid carboxylate groups on the four nitrogen atoms of its twelve-membered cyclic structure. (biomers.net)
  • design
  • The simplest approach to MGB probe design is to use an MGB support, add a quencher molecule as the first addition and complete the synthesis with a 5'-fluorophore. (glenresearch.com)
  • blocks
  • These synthetic systems meet the structural requirements needed to support Darwinian evolution, including a polyelectrolyte backbone, predictable thermodynamic stability, and stereoregular building blocks that fit a Schrödinger aperiodic crystal. (ffame.org)
  • small
  • A human microRNA precursor binding to folic acid discovered by small RNA transcriptomic SELEX. (u-tokyo.ac.jp)
  • clinic
  • To date, there are no inhibitors of complement activation approved for use in the clinic, though certain candidates for clinical use exist, specifically, a recombinant form of complement receptor 1 known as soluble complement receptor 1 (sCR1) and a humanized monoclonal anti-C5 antibody (5G1.1-scFv). (justia.com)
  • complexes
  • Diethylenetriaminepentaacetic acid (DTPA), also known as pentetic acid, is a chelating agent and capable of forming extremely stable complexes with metal salts. (biomers.net)