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  • transgenic
  • Depending on the size of the DNA fragments for integration, 5-15% of the F0 transgenic axolotl are positive for the transgene. (pnas.org)
  • EdU pulse-chase experiments and in vivo tracking of individual transgenic Piwi1 + stem cells showed that the cellular source for blastema formation is migration of stem cells from a remote area. (elifesciences.org)
  • This discovery was made using transgenic newts, the use of which has only recently been made possible. (qubitsnews.com)
  • One of the researchers on the team, Martin Casco-Robles, from the Faculty of Life and Environmental Sciences, University of Tsukuba, is a pioneer in developing techniques for the creation of transgenic newts. (qubitsnews.com)
  • The gene was linked to a red fluorescent protein which could be switched on and off at precise times with the addition of a particular chemical to the rearing solution.Selected transgenic newts had a limb removed under anesthesia. (qubitsnews.com)
  • amphibians
  • Other amphibians with regenerative potential, such as axolotls, lose this ability once they metamorphose from a larva to a juvenile. (qubitsnews.com)
  • In amphibians this process is regulated by nerve fibres present at the site of injury, which have been proposed to release factors into the amputated limbs/fins, promoting and sustaining the proliferation of blastemal cells. (biomedcentral.com)
  • genes
  • Can this remarkable ability be explained by genes shared by vertebrates, including humans, or by unique genes that the newt may have evolved? (eurekalert.org)
  • In the study, the researchers constructed a database of all the protein-coding genes from the Japanese fire-bellied newt that had been reported in the Japan newt research community. (eurekalert.org)
  • Eggs are large and can now be manipulated genetically, permitting the interrogation of specific genes in regenerative processes. (capeia.com)
  • formation
  • Our findings also identify satellite cells as potential targets in promoting mammalian blastema formation. (rupress.org)
  • This process is generally referred to as the dedifferentiation step leading to the formation of blastema progenitors ( Iten and Bryant, 1973 ). (rupress.org)
  • Skeletal muscle is an important contributor to blastema formation ( Brockes, 1997 ). (rupress.org)
  • genome
  • It is therefore inevitable that the sequencing of the axolotl genome is a foremost priority in understanding the cellular mechanisms by which this epimorphic process works. (inquiriesjournal.com)
  • NGS technology provides a viable resource for sequencing the axolotl genome. (inquiriesjournal.com)
  • Here, we report that the CRISPR-Cas9 RNA-guided nuclease system can efficiently create mutations at targeted sites within the axolotl genome. (biologists.org)
  • functional
  • Functional analysis of chick heparan sulfate 6-O-sulfotransferases in limb bud development. (springer.com)
  • These stem cells then created a bud (known as a blastema) that developed into a new, fully functional head within two days, allowing the animals to capture prey. (elifesciences.org)
  • We report that pseudotyped, replication-incompetent retroviruses can be used in axolotls to permanently express markers or genetic elements for functional study. (biologists.org)
  • We have found that pseudotyped Maloney Murine Leukemia Virus (MMLV) retroviruses can very efficiently infect axolotl cells in vitro and in vivo , and can be used for both lineage and functional analyses in regenerating limbs. (biologists.org)
  • protein
  • We screened the blastema of resected axolotl limbs with a cross-reactive mouse cytokine protein detection array, revealing a rapid induction of cytokines, chemokines, and inflammatory markers within 1 d of limb resection. (pnas.org)
  • To understand its function, the researchers investigated where and when the Newtic1 protein is expressed in the newt. (eurekalert.org)
  • occurs
  • Studying the hydrozoan Hydractinia echinata we show that a burst of stem cell proliferation occurs following decapitation, forming a blastema at the oral pole within 24 hr. (elifesciences.org)
  • researchers
  • The researchers inserted a gene known to be active in SMFCs into single-celled newt embryos. (qubitsnews.com)
  • In a study published in Scientific Reports , researchers at several Japanese universities, including the University of Tsukuba, and the University of Dayton, report the discovery of a novel gene, Newtic1, from the blastema of an adult newt. (eurekalert.org)
  • metamorphosis
  • Curiously, axolotls can be induced to undergo a final metamorphosis and rendered terrestrial by administration of the hormone thyroxine. (capeia.com)
  • undergo
  • As neotenous animals, their life cycle differs from that of a protoypical salamander: axolotls do not undergo a final metamorphic stage and take to land. (capeia.com)