• Antisera developed against cultured bacteria from both CLS and CVC plants reacted positively against plant extracts of both diseases in dot immunobinding assays (DIBA). (apsnet.org)
  • Because the particles were assumed to be bacterial pathogens, efforts were made to produce an axenic culture of the ameba isolate, and 16S rRNA PCR was performed to identify any intracellular bacteria. (thefreedictionary.com)
  • Aquatic plants stimulate the growth of and biofilm formation by Mycobacterium ulcerans in axenic culture and harbor these bacteria in the environment. (thefreedictionary.com)
  • 1. The DNA, RNA, protein and carbohydrate contents of myxamoebae of Dictyostelium discoideum strain Ax-2 were measured after growth on bacteria or in various axenic media. (portlandpress.com)
  • The earliest axenic cultures were of bacteria or unicellular eukaryotes, but axenic cultures of many multicellular organisms are also possible. (wikipedia.org)
  • When a microalgae culture contaminated with bacteria passes through the TR tube filled with the triiodide resin, a pure culture of microalgae can be prepared by using the sterilizing rate of bacteria populations, which is relatively higher than that of microalgae, due to characteristics of iodine exhibiting negative polarity. (patentsencyclopedia.com)
  • According to the present invention, the microalgae contaminated with bacteria are sterilized together with bacteria in external air flowing in the TR tube while passing through the TR tube, thereby causing no secondary contamination at the time of inoculation or culture. (patentsencyclopedia.com)
  • 6. A method for culturing axenic culture of microalgae using a triiodide resin, comprising: passing a microalgae culture contaminated with a number of bacteria through a triiodide resin (TR) tube to obtain an axenic culture, wherein the TR tube includes the triiodide resin and has a resin discharge prevention part at a lower portion thereof. (patentsencyclopedia.com)
  • 10. The axenic inoculation system according to claim 9, wherein the microalgae contaminated with bacteria is sterilized by an open triiodide resin (TR) tube. (patentsencyclopedia.com)
  • a Protocols (type of cell used, length of incubation, and culture revelation) were different according to the required bacteria. (asm.org)
  • Insight into the diversity of antibiotic resistance genes in the intestinal bacteria of shrimp Penaeus vannamei by culture-dependent and independent approaches. (bioportfolio.com)
  • Procedures used to prevent the introduction of fungi, bacteria, viruses, mycoplasma or other microorganisms into cell, tissue and organ cultures. (sivb.org)
  • Bacteria associated with cultured rotifers and artemia are detrimental to larval turbot, Scophthalmus maximus L. Aquacultural Engineering 7: 289-293. (springer.com)
  • One method of bacterial culture is liquid culture, in which the desired bacteria are suspended in a liquid nutrient medium, such as Luria Broth , in an upright flask. (wikipedia.org)
  • Liquid cultures are ideal for preparation of an antimicrobial assay in which the experimenter inoculates liquid broth with bacteria and lets it grow overnight (they may use a shaker for uniform growth). (wikipedia.org)
  • Once the growth medium in the petri dish is inoculated with the desired bacteria, the plates are incubated at the optimal temperature for the growing of the selected bacteria (for example, usually at 37 degrees Celsius, or the human body temperature , for cultures from humans or animals, or lower for environmental cultures). (wikipedia.org)
  • Our microbial cultures can confirm the absence of bacteria in samples from germ-free animals. (criver.com)
  • Previous workers reported that Physarum polycephalum amoebae cultured in liquid axenic medium were induced to form cysts by the addition of mannitol. (microbiologyresearch.org)
  • Subculture selection may also involve manually sampling the target organism from an uncontaminated growth front in an otherwise mixed culture, and using this as an inoculum source for the subculture. (wikipedia.org)
  • Harvest cultures and pool when the culture that received the lowest inoculum is at or near peak density. (atcc.org)
  • There was a correlation between nutrient availability and cell yields: the two and three days-old cultures started with 40 × 10 3 cells ml -1 and grown in medium f/2 had lower concentrations than those grown in 2f medium, whereas in cultures with 80 × 10 3 cells ml -1 inoculum the yield was significantly higher only after three days. (bioone.org)
  • Axenic cultivation of E. histolytica was first accomplished by Diamond in 1961 [ 9 ], using a diphasic medium composed of a serum-enriched agar base covered by a broth supplemented with chicken extract and vitamins. (hindawi.com)
  • Transfer of amoebae from liquid culture to the surface of diluted axenic agar medium resulted in the formation of walled cysts identical in appearance with those formed on bacterial lawns. (microbiologyresearch.org)
  • Growth systems to cultivate poplars under axenic conditions in agar and sand cultures with and without mycorrhizal fungi are described. (frontiersin.org)
  • One standard approach with microorganisms is to spread a sample of the culture onto an agar plate, and to incubate this for a fixed period of time. (wikipedia.org)
  • For the purpose of gelling the microbial culture, the medium of agarose gel ( agar ) is used. (wikipedia.org)
  • Microbiological cultures can be grown in petri dishes of differing sizes that have a thin layer of agar-based growth medium. (wikipedia.org)
  • Stab cultures are similar to agar plates, but are formed by solid agar in a test tube. (wikipedia.org)
  • Healthy stems and leaves of ten, 30-day-old plants of S. tragus were spray-inoculated with an aqueous suspension of conidia (1.0 x 106 alpha conidia/ml plus 0.1 percent v/v polysorbate 20) harvested from 14-day-old cultures grown on 20 percent V8 juice agar. (usda.gov)
  • Healthy stems and leaves of ten, 30-day-old plants of S. tragus were spray-inoculated with an aqueous suspension of conidia (1.0 x 106 alpha conidia/ml) harvested from 14-day-old cultures grown on 20 percent V8 juice agar. (usda.gov)
  • The culture collection of algae at Indiana University. (atcc.org)
  • This project will focus primarily on laboratory cultures of axenic marine algae. (epa.gov)
  • A generalized set of conditions for culturing micro-algae (modified from Anonymous, 1991). (fao.org)
  • The production of high value products in micro algae for pharmaceutical use is gaining increased interest, which requires large-scale axenic culture of microalgae. (edu.au)
  • Algae without HKB upregulated their expression of genes coding for ammonium transporters, implying uptake of inorganic nitrogen from the culture medium when prey were unavailable. (biomedcentral.com)
  • donovani strain MHOM/ET/67/L82 were grown in axenic culture at 37 [degrees]C in SM medium (Cunningham 1977) at pH 5. (thefreedictionary.com)
  • 2. Myxamoebae grown in the different axenic media have similar DNA, RNA and protein contents, but there are marked differences in the contents of glycogen and free sugars. (portlandpress.com)
  • The cultures are grown in a sterile synthetic liquid medium devoid of enriched supplements such as serum. (cdc.gov)
  • Axenic Mtb cultures were grown in Sauton's broth, a defined, minimal medium, to late-log phase. (nih.gov)
  • grown for two or three days in f/2 and 2f media were found to be dependent on the initial cell concentration, since the cultures started with 80 × 10 3 cells ml -1 gave better yields than those started with 40 × 10 3 cells ml -1 . (bioone.org)
  • To verify the response of host cells to trichomonad infection, a primary culture of bovine epithelial cells (BOECs) was grown to model host cells, following previously published procedures (Midlej et al. (scielo.br)
  • This study aims to compare the phytochemical composition and pharmacological properties of axenically and naturally grown liverwort L. cruciata to find out whether the axenically cultured bryophytes can be used as a substitute to naturally grown ones to meet up the demands of sample available for use in research purposes. (biomedcentral.com)
  • Term used to denote the maintenance or cultivation of cells in vitro including the culture of single cells. (sivb.org)
  • In obtaining a culture from another laboratory, the proper designation of the culture, as originally named and described, must be maintained and any deviations in cultivation from the original must be reported in any publication. (sivb.org)
  • Method of cultivation: Axenic cultures maitained in liquid medium. (europa.eu)
  • In biology, axenic describes the state of a culture in which only a single species, variety, or strain of organism is present and entirely free of all other contaminating organisms. (wikipedia.org)
  • Axenic culture is an important tool for the study of symbiotic and parasitic organisms in a controlled environment. (wikipedia.org)
  • This may involve use of a dilution series, in which a culture is successively diluted to the point where subsamples of it contain only a few individual organisms, ideally only a single individual (in the case of an asexual species). (wikipedia.org)
  • Such "contaminating" organisms will grow on the plate during this period, identifying cultures that are no longer axenic. (wikipedia.org)
  • citation needed] As axenic cultures are derived from very few organisms, or even a single individual, they are useful because the organisms present within them share a relatively narrow gene pool. (wikipedia.org)
  • In the case of an asexual species derived from a single individual, the resulting culture should consist of identical organisms (though processes such as mutation and horizontal gene transfer may introduce a degree of variability). (wikipedia.org)
  • One set was tested by culture techniques for the presence of live organisms at the Mycoplasma Laboratory, Science Applications International Corporation, National Cancer Institute, Frederick Research and Development Center, Frederick, MD. A commercial nongovernment facility, Charles River Tektagen, tested the second set for mycoplasma DNA by polymerase chain reaction (PCR) assay. (cdc.gov)
  • An axenic culture may include the purposeful cocultivation of different types of cells, tissues or organisms. (sivb.org)
  • A microbiological culture , or microbial culture , is a method of multiplying microbial organisms by letting them reproduce in predetermined culture medium under controlled laboratory conditions. (wikipedia.org)
  • A pure (or axenic ) culture is a population of cells or multicellular organisms growing in the absence of other species or types. (wikipedia.org)
  • Traditional culture-based studies in the past have documented the trophic tendencies of various mixotrophs [ 10 - 13 ], but have not provided details on the molecular processes occurring in the organisms. (biomedcentral.com)
  • Activities of phenol oxidizing enzymes of ectomycorrhizal fungi in axenic culture and in symbiosis with Scots pine (Pinus sylvestris L. (thefreedictionary.com)
  • Comparison of development of axenic cultures of five species of lichen-forming fungi. (wikimedia.org)
  • Motile plasmodia are also found in some cultured species (Okamoto et al. (tolweb.org)
  • Better culture media could facilitate the in vitro study of microorganisms such as E. histolytica . (hindawi.com)
  • Here we report on the optimization of non-infective T. vivax epimastigote axenic cultures and on the process of parasite in vitro differentiation into metacyclic infective forms. (plos.org)
  • The axenic culture of some pathogens is complicated because they normally thrive within host tissues which exhibit properties that are difficult to replicate in vitro. (wikipedia.org)
  • Myriads of elements affect human embryo culture in vitro. (bioportfolio.com)
  • Percent shoot regeneration from leaf explant was highest (86.66%) followed by in vitro source of explant (83.65%), B5 basal medium (83.20%) or 2.27 uM TDZ (73.70%) after 60 days of initial culture. (thefreelibrary.com)
  • Furthermore, we have demonstrated that curcumin inhibited the histone acetyltransferase (HAT) activity of the recombinant PfGCN5 in vitro and reduced nuclear HAT activity of the parasite in culture. (thenakedscientists.com)
  • Axenic culture of L. cruciata was established by propagating gemmae under in vitro condition. (biomedcentral.com)
  • and inoculating the axenic culture into a culture liquid inside the PBR to culture microalgae. (patentsencyclopedia.com)
  • and inoculate the axenic culture in a culture solution inside the PBR to culture microalgae. (patentsencyclopedia.com)
  • [0001] The present invention relates to an axenic inoculation system for microalgae using a triiodide resin (TR) tube of a triiodide resin, and a method for culturing an axenic culture. (patentsencyclopedia.com)
  • More particularly, a TR tube is fabricated using a triiodide resin and attached on an upper portion of a photobioreactor (PBR), and used for passing a culture solution containing microalgae, pure liquid and air through the TR tube. (patentsencyclopedia.com)
  • This project aims to bridge this gap, creating a scalable single-use photobioreactor for the production of axenic microalgae capable of producing high value products. (edu.au)
  • Axenic cultures of microorganisms are typically prepared by subculture of an existing mixed culture. (wikipedia.org)
  • For example, a throat culture is taken by scraping the lining of tissue in the back of the throat and blotting the sample into a medium to be able to screen for harmful microorganisms, such as Streptococcus pyogenes , the causative agent of strep throat. (wikipedia.org)
  • It is often essential to isolate a pure culture of microorganisms. (wikipedia.org)
  • Microbial culture collections focus on the acquisition, authentication, production, preservation, catalogueing and distribution of viable cultures of standard reference microorganisms , cell lines and other materials for research in microbial systematics . (wikipedia.org)
  • Selection of those cultures consisting solely of the desired organism produces the axenic culture. (wikipedia.org)
  • A total of 15 isolates of Giardia intestinalis, the first axenic cultures of this organism to be described from Germany, were established in Bonn from faecal cysts obtained from human and animal stool specimens. (nih.gov)
  • Microbial cultures are used to determine the type of organism, its abundance in the sample being tested, or both. (wikipedia.org)
  • A pure culture may originate from a single cell or single organism, in which case the cells are genetic clones of one another. (wikipedia.org)
  • Here, we describe modified ACCM and culture conditions that support improved growth of C. burnetii and their use in genetic transformation and pathogen isolation from tissue samples. (asm.org)
  • A completely axenic C. burnetii genetic transformation system was developed using ACCM-2 that allowed isolation of transformants in about 2 1/2 weeks. (asm.org)
  • Lichen growth forms cannot be recapitulated in the laboratory by culturing the plant and fungal partners together. (sciencemag.org)
  • [4] [5] Culture collection are also repositories of type strains . (wikipedia.org)
  • The work presented herein focused on determining axenic conditions for culturing and growing insect (epimastigote) forms of T. vivax and prompting their differentiation into metacyclic forms that are infectious for the mammalian host. (plos.org)
  • However, a major impediment to validating this model is a poor understanding of the actual nutrient conditions encountered by M. oryzae during infection, what nutrients can be acquired from the host, and how closely axenic growth in synthetic minimal media mimics the nutrient conditions of the plant. (esiservizi.com)
  • Bacterial additives that consistently enhance rotifer growth under synxenic culture conditions 2. (springer.com)
  • A total of 21 out of 79 amoeba peptidase genes analysed were found to be significantly expressed under standard axenic culture conditions whereas the remaining are not expressed or at very low levels only. (biomedcentral.com)
  • Under standard culture conditions or upon heat-stress only a relatively small number of these genes is significantly expressed and only very few variations become apparent between various clinical E. histolytica isolates, calling into question the importance of these enzymes in E. histolytica pathogenicity. (biomedcentral.com)
  • Appropriate culture conditions, media, and the effect of hormones on growth and development were studied. (biomedcentral.com)
  • Although culturing plant tissues and organs under axenic conditions was first established and profitably employed in bryophytes, especially mosses [ 13 ], bryophytes did not retain for long their rightful place as a highly favored research object. (biomedcentral.com)
  • Continuous growth of bloodstream forms of Trypanosoma brucei brucei in an axenic culture system containing a low concentration of serum. (thefreedictionary.com)
  • In any published description of a culture, one must make every attempt to publish the characterization or history of the culture. (sivb.org)
  • A cell line arises from a primary culture at the time of the first successful subculture. (sivb.org)
  • Development of media formulations permitting axenic culture of marine pathogens has enabled scientists to study the growth of parasites, their virulence factors and host-parasite interactions. (thefreedictionary.com)
  • We present a new culture medium for Entamoeba histolytica which allows the microbe to preserve its virulence factors and ability to induce hepatic abscesses in animal models. (hindawi.com)
  • In 1988, our group reported an in-house developed axenic culture medium, PEHPS [ 14 ]. (hindawi.com)
  • The axenic culture medium 199 supplemented by 5% of calf newborn serum was used. (thefreedictionary.com)
  • About 100 [micro]l of the culture medium with 105 amastigotes from axenic culture with or without a serial drug dilution was seeded in 96-well microtiter plates. (thefreedictionary.com)
  • Immediately after thawing, aseptically remove the contents of the ampule and inoculate into 5 mL of fresh ATCC medium 712 in a T-25 tissue culture flask or plastic 16 x 125 mm screw-capped test tube. (atcc.org)
  • Incubate the culture at 35°C with the cap screwed on tightly (tube should be vertical for medium 359 or on a 15° horizontal slant for medium 2154). (atcc.org)
  • NMII was successfully cultured in ACCM-2 when medium was inoculated with as little as 10 genome equivalents contained in tissue homogenates from infected SCID mice. (asm.org)
  • KEYWORDS: biomass production, Culture medium, Dunaliella sp. (bioone.org)
  • There was, however, a significant difference in the average cell size, with diameters ranging from 9.30 +/- 0.184 to 11.11 +/- 0.22 microns and from 9.17 +/- 0.05 to 11.25 +/- 0.24 microns for cells growing in Excell 401 serum-free medium in spinner flask cultures and in TNM-FH medium supplemented with 10% FBS in tissue flask cultures, respectively. (biomedsearch.com)
  • Host cell cultures were exposed to a parasite:BOECS cell ratio of 5:1 for 12 h in 37ºC ( Fig. 1C, D ). Cells were equilibrated in incubation medium for 5 min at 37ºC (5% CO 2 ) prior to parasite addition. (scielo.br)
  • Microbial cultures. (uv.es)
  • Although these procedures are used to prevent microbial contamination of cultures, they also prevent cross contamination of cell cultures as well. (sivb.org)
  • Microbial cultures are foundational and basic diagnostic methods used extensively as a research tool in molecular biology . (wikipedia.org)
  • Anaerobic agents are the most difficult to detect from oxygen-containing environments and, therefore, it is critical that anaerobic collection culture media, anaerobic chambers, and powerful microbial identification technologies be utilized to ensure accurate results. (criver.com)
  • The aim of this study was to determine whether T. foetus and T. vaginalis stimulated the release of the cytokine interleukin (IL)-10 from cultured bovine epithelial cells. (scielo.br)
  • To characterise the inflammatory response induced by these parasites, primary cultures of bovine oviduct epithelial cells were exposed to either T. vaginalis or T. foetus. (scielo.br)
  • A morphological description must await achieving axenic culture , but the partial sequence of the SSUrDNA has been given the GenBank accession numbers AF228362 and AF228363 for the V1, and V2 and V3 regions of the SSUrDNA, respectively. (thefreedictionary.com)
  • The vaccine, which is administered subcutaneously over an 18-month schedule, is derived from sterile, acellular filtrates of microaerophilic cultures of the avirulent, nonencapsulated V770-NP1-R strain of Bacillus anthracis . (cdc.gov)
  • strain PWH3a was infected with a lytic virus (PWH3a-P1) and the resulting 36.0 µ mol L −1 of dissolved organic N (DON) in the lysate was added to cultures containing cyanobacteria ( Synechococcus sp. (biogeosciences.net)
  • Clonal propagation through tissue culture offers an alternative approach to vegetative propagation. (thefreelibrary.com)
  • The recent description of a method for axenic (host cell-free) propagation of C. burnetii is a significant technological advance that will facilitate biochemical and genetic studies of the pathogen ( 22 ). (asm.org)
  • Molecular detection of Coxiella burnetii in heart valve tissue from patients with culture-negative infective endocarditis. (pubfacts.com)
  • Coxiella burnetii is a common cause of blood culture-negative infective endocarditis (IE). (pubfacts.com)
  • Therefore, the search for better culture media and low-cost diagnostic and therapeutic tools is fundamental. (hindawi.com)
  • To compare the performance of three-culture media (traditional TYI-S-33, PEHPS, and CLUPS), E. histolytica trophozoites were cultured in quintuplicate, followed by the evaluation of phospholipase activity and the induction of liver abscesses in golden hamsters. (hindawi.com)
  • This unit includes protocols for culturing worms on solid and axenic media and acute and chronic exposure parameters for Parkinson's disease-associated toxins and hemin chloride. (currentprotocols.com)
  • Various specific recipes for algal culture media are described by Vonshak (1986). (fao.org)
  • The complexity and cost of the above culture media often excludes their use for large-scale culture operations. (fao.org)
  • The availability of the complete genome sequence of the nematode C. elegans , coupled with the worm's size, growth rate, ease of culturing, and the realization that basic biological mechanisms and disease processes between worms and humans are highly conserved, makes this genetically tractable model a remarkable opportunity to dissect and identify in vivo the cellular processes involved in toxin‐induced cell dysregulation and death. (currentprotocols.com)
  • Here, we examined the diagnostic utility of Q fever polymerase chain reaction (PCR) of formalin-fixed heart valve tissue from patients with blood culture-negative IE who underwent heart valve surgery. (pubfacts.com)
  • In tissue culture, cytokinins play an important role as they promote cell division and develop meristematic centers leading to the formation of organs, mainly shoots (Peeters et al. (thefreelibrary.com)
  • 2006). Even HeLa cells cultured with this parasite have been reported to secrete some cytokines (Chang et al. (scielo.br)
  • The data indicate that genetically based metabolic differences may determine how rapidly G. intestinalis isolates can grow in axenic culture. (nih.gov)
  • Nitrogen flow in a chemostat culture of the rotifer Brachionus plicatilis . (springer.com)
  • The role played by specific probiotic candidates is still indistinct and further research is required to ascertain their significance in rotifer culture. (springer.com)
  • In this study, euryhaline rotifer Brachionus rotundiformis (SS-type) was used to evaluate the effect of two commercial probiotic products on rotifer density, reproductive parameters and unionized ammonia concentration for a twelve-day culture period. (springer.com)
  • Ideally, cultured parasites should present the same growth and virulence characteristics as their counterparts in their natural habitat [ 8 ]. (hindawi.com)
  • The supernatants of cultures with parasites or without (controls) were collected and cytokine levels were quantified with specific kits for measuring IL-10 (Becton Dickinson, Franklin Lakes, NJ, USA), which were used according to the manufacturers' instructions. (scielo.br)
  • They are digenetic parasites: the flagellated promastigote forms, which can be derived in axenic culture, differentiate within the alimentary tracts of sandfly vectors from a replicating procyclic to a nonreplicating, infectious metacyclic stage, whereas obligately intracellular amastigotes live and replicate in mammalian mononuclear phagocytes, such as macrophages ( 1 ). (asm.org)
  • Diagnosis is based on serologic analysis, culture, and molecular biology. (cdc.gov)
  • Integrate a source of irradiance, optimised gas (CO2/O2) exchange and temperature control into GE Healthcare's Xcellerex bioreactor platform, set up a flexible sensor array for monitoring of the culture. (edu.au)
  • Cattle infectious subspecies in axenic asynchronous culture. (lshtm.ac.uk)
  • Charles River offers comprehensive, accurate, and sensitive detection of infectious agents using both aerobic and anaerobic cultures of samples obtained from the isolator environment, individual cages, and directly from colony animals. (criver.com)
  • 1978. Method for the simultaneous establishment of many axenic cultures of Paramecium . (springer.com)
  • In cell cultures, the cells are no longer organized into tissues. (sivb.org)
  • Here we show that curcumin , a polyphenolic organic molecule derived from turmeric, inhibits chloroquine-resistant Plasmodium falciparum growth in culture in a dose dependent manner with an IC(50) of approximately 5 microM. (thenakedscientists.com)
  • More particularly, a TR tube is fabricated using a triiodide resin and a method for culturing an axenic culture. (patentsencyclopedia.com)
  • The Effect of Initial Cell and Nutrient Concentrations on the Growth and Biomass Production of Outdoor Cultures of Dunaliella sp. (bioone.org)
  • The final concentrations of non-axenic outdoor mass cultures of Dunaliella sp. (bioone.org)
  • Concentrations of cells in phytoplankton cultures are generally higher than those found in nature. (fao.org)
  • Shell-vial culture using the JNSP protocol from January 1996 to September 2003. (asm.org)
  • In a re-association experiment, cells from the heterotrophic culture were added to an axenic culture of the Anabaena sp. (thefreedictionary.com)
  • 5. The rate of amino acid oxidation during axenic growth of the myxamoebae is decreased when the cells are supplied with glucose. (portlandpress.com)
  • Harvest cells from a culture that is at or near peak density by centrifugation at 700-800 x g for 5 min. (atcc.org)
  • There is hardly a field of biological investigation in which culturing of such cells is not employed. (sivb.org)
  • Cells, or cultures derived from them, which will grow, survive, or maintain function only when attached to a surface such as glass or plastic. (sivb.org)
  • The percentage of cells plated (seeded, inoculated) which attach to the surface of the culture vessel within a specified period of time. (sivb.org)
  • The term cell line implies that cultures from it consist of lineages of cells originally present in the primary culture. (sivb.org)
  • Batch culture experiments without the flagellate demonstrated that the elongation of C. acidovorans PX54 cells was dependent on their growth rate. (asm.org)
  • There are several types of bacterial culture methods that are selected based on the agent being cultured and the downstream use. (wikipedia.org)
  • Improved Methods for the Axenic Culture of Puccinia recondita f. sp. (apsnet.org)
  • To date, nine anaerobic fungal genera have been described, with further novel taxonomic groupings known to exist based on culture-independent molecular surveys. (frontiersin.org)
  • To clarify the molecular bases of this metabolic feature, we investigated light-driven lipid biosynthesis in Nannochloropsis gaditana cultures combining the analysis of photosynthetic functionality with transcriptomic, lipidomic and metabolomic approaches. (plantphysiol.org)
  • Moreover, skin microbiome studies have heavily relied on 16S rRNA sequencing data in the absence of bacterial culture. (bioportfolio.com)
  • Likewise, CLUPS-cultured trophozoites produced significantly more phospholipases than TYI-S-33-cultured trophozoites. (hindawi.com)
  • An E. festucae Δ bemA mutant was defective in hyphal morphogenesis and growth in culture and in planta . (pnas.org)
  • This confusion is especially acute in the field of vertebrate, invertebrate and plant cell culture. (sivb.org)
  • Incubate the culture at 50-100 µEinsteins/m 2 /s irradiance at 25°C. Maintain under a 14/10h light-dark photoperiod. (atcc.org)
  • The live food(s) would be cultured in a dedicated vessel. (wetwebmedia.com)
  • they were cultured without flagellates in the first phase of the experiments and in the presence of the flagellates in the second phase. (asm.org)
  • Our goals were to further annotate the A. apis reference genome and to identify genes that are candidates for being differentially expressed during host infection versus axenic culture. (biomedsearch.com)