Avoid repeated freeze determines cell cycle. Medical search. Frequent questions

Avoid repeated freeze-thaw cycles. (sputnic-group.ru)
Store at -20°C. Avoid repeated freeze/thaw cycles. (avivasysbio.com)
As with all antibodies care should be taken to avoid repeated freeze thaw cycles. (prosci-inc.com)
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. (watson-bio.cn)
Please minimize freeze-thaw cycles. (biorbyt.com)

Attenuate hepatic lipogenesis and gluconeogenesis, regulate NO production in endothelial cells, inhibit proliferation of smooth muscle cells and prevent lipid accumulation of macrophage cells. (prospecbio.com)
ED50= 3-8.5 μg/ml, as determined by its ability to inhibit proliferation of HASMCs induced by HB EGF. (prospecbio.com)
IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. (watson-bio.cn)
Determined by the dose-dependent stimulation of the proliferation of AML5 cells. (biofargo.com)
FLT3 ligand is a growth factor that regulates the proliferation of early hematopoietic cells. (biofargo.com)
The FLT3 ligand itself does not stimulate the proliferation of early hematopoietic cells, but rather synergistically induces growth and differentiation with other CSFs and interleukins. (biofargo.com)
Recombinant human PTN (HZ-1278) stimulates dose-dependent proliferation of the THP-1 monocyte cell line. (ptglab.com)
In cell coculture assays, ICOVIR-15K-cBiTE-mediated oncolysis resulted in robust T-cell activation, proliferation, and bystander cell-mediated cytotoxicity. (aacrjournals.org)
Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. (cusabio.com)
The ED(50) was determined by the dose-dependent stimulation of the proliferation ofmurineTD1cells was found to be less than.1 ng/ml, corresponding to specific activity of 1x10 7 IU/mg. (reprokine.com)
In SIVagm infection, systemic IL-15 was associated with NK cell proliferation in lymph node (LN), while systemic IFNα correlated with NK cell cytotoxicity in LN 17 . (nature.com)

Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. (avivasysbio.com)
Pleiotrophin is a secreted growth factor that induces neurite outgrowth being mitogenic for fibroblasts, epithelial, and endothelial cells. (ptglab.com)
The ED(50) was determined by the dose-dependentproliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of.0-10.0 ng/ml. (reprokine.com)
The hemostatic system consists of platelets, coagulation factors, and the endothelial cells lining the blood vessels. (medscape.com)
Under physiological circumstances, the resistance of the endothelial cell lining to interactions with platelets and coagulation factors prevents thrombosis. (medscape.com)
[ 2 ] Diagnosed in 50% of patients with VHL disease, these hemangiomas are composed of endothelial cells and pericytes. (medscape.com)

Optimal working dilutions must be determined by end user. (sputnic-group.ru)
In a starting experiment, we recommend determining optimal concentrations of antibiotic required to kill your host cell line. (invivogen.com)
If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their particular experiments. (antikoerper-online.de)
Optimal dilutions should be determined by the end user. (avivasysbio.com)
Optimal dilutions for each application to be determined by the researcher. (prosci-inc.com)
Optimal conditions must be determined individually for each application. (enzolifesciences.com)
Please Note: Optimal dilutions should be determined by each laboratory for each application. (rndsystems.com)

Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity. (enzolifesciences.com)
In this study, we tested the hypothesis that tumor-infiltrating T cells could be more effectively activated and redirected by oncolytic adenoviruses that were armed with bispecific T-cell-engager (BiTE) antibodies. (aacrjournals.org)
[ 6 ] Presence of these other antibodies is helpful, as they determine the course of disease and also explain the variable response to universal treatment modalities for MG. (medscape.com)

Trimeric form of Adiponectin Human trimeric form was expressed in HEK293 cells. (prospecbio.com)
Human HGF, His tag, is expressed in HEK293 cells. (creative-bioarray.com)

Store lyophilized Adiponectin at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. (prospecbio.com)

The purity of Human ACE2 (18-652) Recombinant Protein (hFc-Tag) was determined by densitometry after SDS-PAGE of 2 μg of protein, followed by staining with Coomassie Blue. (cellsignal.com)
Purity of recombinant human PTN was determined by SDS- polyacrylamide gel electrophoresis. (ptglab.com)
The purity is 85%+ determined by SDS-PAGE. (cusabio.com)

J Cell Biol. (sputnic-group.ru)
Mol Cell Biol. (watson-int.cn)

The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (uscnk.com)
Less than 0.1 EU per µg protein as determined by the LAL method. (biolegend.com)
The protein encoded by this gene is the fourth major glycoprotein of the platelet surface and serves as a receptor for thrombospondin in platelets and various cell lines. (origene.com)
Since thrombospondins are widely distributed proteins involved in a variety of adhesive processes, this protein may have important functions as a cell adhesion molecule. (origene.com)
Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). (avivasysbio.com)
Western blot sample preparations, including lysis buffers, lysate from cell culture, lysate from tissues and determination of protein concentration. (abcam.com)
The tissue suspensions were sonicated well and centrifuged in a TL-100 ultracentrifuge (Beckman Instruments, Fullerton, CA) at 100,000 g for 1 h at 25°C, and the protein concentrations in the supernatants were determined. (sigmaaldrich.com)
This is a fast, easy, and direct approach for expression in cell-free protein synthesis systems using linear DNA templates. (jove.com)
Cell-free protein synthesis (CFPS) has recently become very popular in the field of synthetic biology due to its numerous advantages. (jove.com)
Cell-free protein synthesis (CFPS) systems are increasingly being used as a fast, simple, and efficient method for biosensor engineering, decentralized manufacturing, and prototyping of genetic circuits 1 . (jove.com)
The main bioactive form is anchored on the cell surface as the extracellular domain of transmembrane protein (209 a. (biofargo.com)
By putting the IL10 gene into the genetic material of Mammalian cell cell, the Mammalian cell could be used as factories or producers to make the desired IL10 protein for research uses. (cusabio.com)
The foamy stromal cells between the capillaries stain positive for glial fibrillary acid protein and neuron-specific enolase. (medscape.com)

The interaction of FAS/FASL in immature dendritic cells (DCs) induces functional maturation. (biolegend.com)
It is expressed by CD4+ and CD8+ T cells, gammadelta T cells, B cells, dendritic cells, and eosinophils. (watson-bio.cn)
IL-2 is expressed by CD4+ and CD8+ T cells, gamma δ T cells, B cells, dendritic cells, and eosinophils (1-3). (rndsystems.com)
IL10-mediated inhibition of IFN-γ secretion by Th1 cells was demonstrated to occur via its action on the APC function of macrophages and by its inhibition of cytokine production by activated macrophages and dendritic cells. (cusabio.com)

Typically, mammalian cells are sensitive to blasticidin concentrations of 1-10 µg/ml, and bacteria to 25-100 µg/ml. (invivogen.com)
1991. Blasticidin S-resistance gene (bsr): A novel selectable marker for mammalian cells. (invivogen.com)
1994. Blasticidin S deaminase gene from Aspergillus terreus (BSD): a new drug resistance gene for transfection of mammalian cells. (invivogen.com)
The working concentration of blasticidin for mammalian cell lines varies from 1 to 10 μg/ml, in a few cases up to 30 μg/ml. (invivogen.com)
Suggested concentrations of blasticidin for selection in some examples of mammalian cells are listed below. (invivogen.com)
2011. Streamlined platform for short hairpin RNAinterference and transgenesis in cultured mammalian cells. (invivogen.com)

FLT 3-ligand binds to cells expressing tyrosine kinase receptor FLT3. (biofargo.com)
This syrup prevents the inside of the cell from freezing and binds water molecules to prevent dehydration. (fws.gov)

HEK293 (Human embryonic kidney cell line). (prospecbio.com)
EFNA1 Human Recombinant produced in HEK293 cells is a single, glycosylated polypeptide chain (a.a 19-182) containing 170 amino acids including a 6 a.a C-terminal His tag. (prospecbio.com)
HEK293 cells. (prospecbio.com)

Proteins that are found exclusively or predominantly in a sub-cellular location will be more enriched in a lysate of the sub-cellular fraction compared with whole cell or tissue lysates. (abcam.com)
We present the preparation steps of cell lysate from Escherichia coli BL21 Rosetta2 ΔrecBCD strain by sonication lysis and buffer calibration for Mg-glutamate (Mg-glu) and K-glutamate (K-glu) specifically for linear DNA. (jove.com)

They are recruited from the cytosol onto cell membranes in the inactive form, are activated by guanine nucleotide exchange factors and then bind a large number of Rab effector proteins. (sputnic-group.ru)
Rab10, first isolated from a clone of Madin-Darby Canine kidney (MDCK) cells belongs to a subfamily of Rab proteins that includes Rab8 and Rab13. (sputnic-group.ru)
Wnt family proteins have been implicated in oncogenesis and in multiple developmental processes including regulation of cell fate and patterning during embryogenesis. (biolegend.com)
This is a popular buffer for studying proteins that are cytoplasmic or membrane-bound, or for whole cell extracts. (abcam.com)
RIPA buffer is useful for whole cell extracts and membrane-bound proteins, and may be preferable to NP-40 or Triton X-100-only buffers for extracting nuclear proteins. (abcam.com)
Wood frogs have special proteins in their blood that causes it to freeze first. (fws.gov)
Water 2H and 17O spin relaxation is used to study water dynamics in the hydration layers of two small peptides, two globular proteins and in living cells of two microorganisms. (lu.se)

For the quantitative determination of rabbit HDL concentrations in serum, plasma,cell culture supernates and tissue homogenate. (antikoerper-online.de)
Linear DNA can be rapidly and easily amplified by PCR to obtain high concentrations of the template, avoiding potential in vivo expression toxicity. (jove.com)
THP-1 cells were treated with increasing concentrations of recombinant PTN for 120 hours. (ptglab.com)
For the quantitative determination of human ODC concentrations in serum, plasma,cell culture supernates and tissue homogenate. (anticorps-enligne.fr)
There is no significant difference in cell water dynamics between mesophilic and halophilic organisms, despite the high K+ and Na+ concentrations in the latter. (lu.se)
BACKGROUND: Current studies examining the effects of high concentrations of red blood cell (RBC) or serum folates assume that high folate concentrations are an indicator of high folic acid intakes, often ignoring the contributions of other homeostatic and biological processes, such as kidney function. (cdc.gov)

The vasopressin (VP) magnocellular neurosecretory cells (MNCs) in the supraoptic and paraventricular (PVN) nuclei are regulated by estrogen and exhibit robust expression of estrogen receptor (ER)-β. (jneurosci.org)
Because of the eminent role of receptors for mediating the biological effects of steroid hormones, changes in receptor expression could alter responsiveness of the target cells and determine the physiological circumstances in which gonadal steroids become important regulators of cell function. (jneurosci.org)
The receptor for IL-2 consists of three subunits (55 kDa IL2Ralpha, 75 kDa IL2Rβ, 64 kDa common gamma chain gammac/IL2Rgamma) that are present on the cell surface in varying preformed complexes. (watson-bio.cn)
The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes (5-7). (rndsystems.com)
On the cell surface, the receptor for this chemokine is CXCR4 and syndecan4. (watson-bio.cn)
define NK cell differentiation states based on their education via NKG2a and expression of CD16 (FcγRIII), an activating Fc receptor that mediates antibody-dependent cell-mediated cytotoxicity (ADCC) 8 , and demonstrate that nonpathogenic SIVagm infection in African green monkeys (AGMs) imprints the maturation of NK cells inducing terminally differentiated NKG2a low CD16 + NK cells, which express high levels of interleukin (IL)−21R. (nature.com)

Ferroportin disease (hemochromatosis type 4) is a recently recognized disorder of human iron metabolism, characterized by iron deposition in macrophages, including Kupffer cells. (intrinsiclifesciences.com)
In patients with most, but not all, ferroportin mutations, retention of iron in macrophages of the liver and other organs may protect against damage to parenchymal cells. (intrinsiclifesciences.com)

In most cases it is possible to seed CHO cells that have been removed from freezing medium directly in the serum-free medium, when the cell concentration is at least 5x10 5 cells per 25cm 2 . (bioind.com)
The cells should be seeded with serum-free medium containing 5% serum and the serum concentration is then gradually reduced with each passage. (bioind.com)
Then centrifuge, suspend the cells in serum-free medium and transfer them in the desired concentration. (bioind.com)
Simply enter the mass of reagent and the target concentration and the calculator will determine the rest. (rndsystems.com)
The biological activity determined by a chemotaxis bioassay using rat neutrophils is in a concentration range of 10-100 ng/ml. (watson-int.cn)
The biological activity determined by a chemotaxis bioassay using PHA and rHuIL-2 activated human peripheral blood T-lymphocytes is in a concentration range of 20-80 ng/ml. (watson-bio.cn)
Pb) are known to be associated mostly with the red blood cells in the specimen and a uniform distribution of this cellular material must be produced before a small volume extracted from the larger specimen will accurately reflect the average metal concentration of all fractions of the larger specimen. (cdc.gov)

Frostbite is a skin injury that occurs when exposed to extreme low temperatures, causing the freezing of the skin or other tissues, commonly affecting the fingers, toes, nose, ears, cheeks and chin areas. (wikipedia.org)
In third degree frostbite, the layers of tissue below the skin freeze. (wikipedia.org)
Our fragile human bodies are susceptible to frostbite in freezing temperatures, so we layer up to protect ourselves. (fws.gov)

Flow cytometry analysis of Daudi cells with anti-CD22 antibody (Cat#HPAB-0709-FY, Blue line) or isotype control antibody (Yellow line). (creativebiolabs.net)
When samples are prepared using the STAINperfect immunostaining kit A , our antibody ensures clear-cut Octopamine stainings in whole mounts, cell culture and tissue sections. (immusmol.com)

BIOCHO-1 SFM Base is the basic formulation for CHO cells. (bioind.com)
Note: NK cell activation reagent Product Information Activity NK cells activated with this product have a cytotoxic index (T-C) of at least 15% Formulation Lyophilized Stability & Storage Samples are stable. (biofargo.com)

Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. (ptglab.com)

IL-10 was discovered 30 yr ago as a secreted cytokine synthesis inhibitory factor, produced by T helper (Th) 2 cell clones shown to inhibit cytokine production by Th1 cells. (cusabio.com)

Mouse Wnt3a induces Topflash reporter activity in HEK293T human embryonic kidney cells with ED 50 range of 7 - 35 ng/mL. (biolegend.com)
It drives resting T cells to proliferate and induces IL-2 and IL-2 R alpha synthesis (1, 2). (rndsystems.com)

Furthermore, because of the antigenic and genetic diversity of 'Norwalk-like viruses'* (NLVs) and the inability to cultivate these viruses in cell lines, developing assays to detect the full spectrum of NLVs associated with outbreaks of gastroenteritis was not possible. (cdc.gov)

It is not in the differentiated cells of most adult tissues. (hatinhibitor.com)
In the index case, the disorder is characterized by abundant deposition of hemosiderin in all tissues investigated (mesenteric lymph node, liver, gastric and duodenal mucosa, and also in squamous cell carcinoma of the lung). (intrinsiclifesciences.com)
These data demonstrate that African green monkey-like natural killer cell differentiation profiles can be rescued in rhesus macaques to promote viral clearance in tissues. (nature.com)

Prevention consists of wearing proper, fully-covering clothing, avoiding low temperatures and wind, maintaining hydration and nutrition, and sufficient physical activity to maintain core temperature without exhaustion. (wikipedia.org)

Notably, intratumoral injection of this cBiTE-expressing adenovirus increased the persistence and accumulation of tumor-infiltrating T cells in vivo , compared with the parental virus lacking such effects. (aacrjournals.org)
For example, ex vivo IL-21 treatment expanded CD16 + NK cells 9 , antagonized the IL-15-dependent expansion of resting NK cells 10 , and reverses hypo-responsiveness via the STAT1 and PI3K-AKT-FOXO1 pathways 11 . (nature.com)
furthermore, in vivo IFNα-induced NK cell cytotoxicity correlates with reductions in HIV-DNA during antiretroviral therapy (ART) 16 . (nature.com)

Serum-Free Cell Freezing Medium (Cat. (bioind.com)
This solution is used for the cryopreservation of cells, which are to be grown in serum-free growth medium. (bioind.com)
The use of this medium, rather than serum-containing freezing media, avoids the necessity of repeated passages in order to remove serum residues. (bioind.com)
The stage at which serum is completely removed is determined in the course of the weaning for each specific case. (bioind.com)
After successful adaptation, it is recommended to cryopreserve the cells in Serum-Free Freezing Medium, in order to avoid the necessity of any further adaptation in the future. (bioind.com)
In addition, the excess trypsin can be neutralized with SBTI, thus avoiding the introduction of serum. (bioind.com)
The US National Institute of Standards and Technology (NIST) developed a Standard Reference Material (SRM) 3949 Folate Vitamers in Frozen Human Serum to replace SRM 1955 Homocysteine and Folate in Human Serum. (cdc.gov)

Wnt-3a mediates self-renewal of the stem cells at the bottom on intestinal crypts. (biolegend.com)
It is a cell adhesion molecule that mediates interactions between spermatogenic cells and Sertoli cells. (rndsystems.com)

Blood lead levels measured in previous NHANES cycles have been the cornerstone of lead exposure surveillance in the U.S. The data have been used to document the burden and dramatic decline of elevated blood lead levels, to promote the reduction of lead use, and to help to redefine national lead poisoning prevention guidelines, standards, and abatement activities. (cdc.gov)
DESIGN: Cross-sectional data from the National Health and Nutrition Examination Survey (NHANES) collected in 2-year cycles were combined from 2011 to 2018. (cdc.gov)

This is in part because the high-quality, fresh-frozen human brain tissue from patients with COVID-19 needed for single-cell transcriptomic studies is largely inaccessible, and methods to isolate human brain barrier cells have only recently emerged 22 , 23 . (nature.com)
Fig. 1: Overview of diverse brain and choroid plexus cell types captured from post-mortem tissue from patients with COVID-19. (nature.com)
Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. (cusabio.com)

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. (biolegend.com)
Background: GNL3 is found in the nucleoli of embryonic stem cells, adult CNS stem cells, primitive cells in the bone marrow and cancer cells. (hatinhibitor.com)

Unlike HIV infection, which progresses to AIDS absent suppressive anti-retroviral therapy, nonpathogenic infections in natural hosts, such African green monkeys, are characterized by a lack of gut microbial translocation and robust secondary lymphoid natural killer cell responses resulting in an absence of chronic inflammation and limited SIV dissemination in lymph node B-cell follicles. (nature.com)

cBiTE expression and secretion was detected in supernatants from ICOVIR-15K-cBiTE-infected cells, and the secreted BiTEs bound specifically to both CD3 + and EGFR + cells. (aacrjournals.org)

For women who have regular menstrual cycles, having sexual intercourse every other day during the "fertile window" can increase the chances for conception. (mountsinai.org)
Abnormal uterine bleeding (AUB) in patients of reproductive age is a bleeding pattern that is not consistent with normal menstrual cycle parameters (frequency, regularity, duration, and volume). (msdmanuals.com)

Inhibition of cytotoxic effect of FASL on Jurkat cells in the presence of recombinant human FAS. (biolegend.com)
FAS was initially purified and cloned from SKW6.4 cells and a cDNA library of human T cell lymphoma KT-3 cells. (biolegend.com)
In addition, FAS/FASL interaction increases the production of chemokines in NK-T cells, and FASL acts as a survival factor for human CD34 + cells and increases their colony formation. (biolegend.com)
Human FAS, amino acids (Arg17-Asn173) (Accession# NM_000043), was expressed in 293E cells. (biolegend.com)
Using this combination approach, we found that human laryngeal carcinoma (HEp-2) cells were superior to rhesus monkey kidney (LLC-MK2) cells commonly used in previous studies for isolation of HMPV. (cdc.gov)
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Here we report, using the pathogenic model of antiretroviral therapy-treated, SIV-infected rhesus macaques that sequential interleukin-21 and interferon alpha therapy generate terminally differentiated blood natural killer cells (NKG2a/c low CD16 + ) with potent human leukocyte antigen-E-restricted activity in response to SIV envelope peptides. (nature.com)

Detection of IL‑2 in PBMC cells by Flow Cytometry. (rndsystems.com)

Given previous findings on a role of IL-21 and IFNα in regulating NK cell function, and that NKG2a low CD16 + NK cells are generated while expressing high levels of IL-21R in nonpathogenic SIV infection, we sought to determine whether immunotherapy with IL-21 and IFNα rescues AGM-like profiles of NK cell maturation and activity in SIV-infected rhesus macaques (RMs). (nature.com)

Cell lysis with detergent-free buffer is achieved by mechanical shearing, often with a Dounce homogenizer or by passing cells through a syringe tip. (abcam.com)
In this protocol, steps for the preparation of cell-free lysates from the E. coli BL21 Rosetta2 ΔrecBCD strain by sonication lysis are described in detail. (jove.com)

cells of monocytic macrophage lineage-mainly and conspicuously Kupffer cells within the liver. (intrinsiclifesciences.com)

Please filter the product by an appropriate sterile filter before using it in the cell culture. (prospecbio.com)
Influenced by factors including cell viability, cell number and cell sampling time, samples from cell culture supernatant may not be recognized by the kit. (antikoerper-online.de)
For testing in cell culture applications, please filter before use. (origene.com)
Do not reconstitute in cell culture media directly. (watson-bio.cn)
These widely available HEp-2 cells should be included in conjunction with a molecular method for cell culture followup to detect HMPV, particularly in patients with SARS. (cdc.gov)
Recombinant IL-2 is widely used in cell culture for the expansion of T cells. (rndsystems.com)
Chinese hamster ovary) cell suspension culture and purified by chromatography including specific viral inactivation and removal procedures. (who.int)

It has been known for a long time that the exonuclease V complex (RecBCD) degrades linear DNA in cell lysates 4 . (jove.com)
Recently, we showed that linear DNA can be much better protected in lysates from cells knocked out for exonuclease genes ( recBCD ) 10 . (jove.com)

Sterile filtered white lyophilized (freeze-dried) powder. (watson-bio.cn)

Presented here is a protocol for the preparation and buffer calibration of cell extracts from exonuclease V knockout strains of Escherichia coli BL21 Rosetta2 ( ΔrecBCD and ΔrecB ). (jove.com)

PBMC stimulated with 1 μg/mL of aCD3 and 3 ug/ml of aCD28 for 2 days were treated with Tocris cell activation cocktail 500x (Catalog # 5476 ) and Brefeldin A (Catalog # 1231/5 ) for 3 hours or naïve PBMC lymphocytes. (rndsystems.com)

HER2 overexpression or HER2 gene amplification as determined by an accurate and validated assay (see sections 4.4 and 5.1). (who.int)

Clinical hallmarks of VHL disease include the development of retinal and central nervous system (CNS) hemangioblastomas (blood vessel tumors), pheochromocytomas , multiple cysts in the pancreas and kidneys, and an increased risk for malignant transformation of renal cysts into renal cell carcinoma. (medscape.com)

Mouse Wnt-3a, amino acids Ser19-Lys352 (Accession # P27467) was expressed in CHO cells. (biolegend.com)
3,8,10,11 Deletion of this amino acid causes loss of function and when studied in vitro causes accumulation of iron in cultured cells. (intrinsiclifesciences.com)

Wnt-3a is a highly conserved lipid-modified, secreted hydrophobic glycoprotein with conserved 24 cysteine residues that is essential for cell signaling. (biolegend.com)

Cell death induced by blasticidin occurs rapidly, allowing for selection of transfected cell lines carrying a blasticidin resistance gene within one week. (invivogen.com)
After treatment, cell death occurs rapidly, allowing the selection of transfected cells with plasmids carrying the bsr or BSD genes in as little as 7 days post-transfection. (invivogen.com)
When this abnormal process occurs repeatedly, the endometrium can become hyperplastic, sometimes with atypical or cancerous cells. (msdmanuals.com)

These media are intended for the growth of CHO cells of various kinds: CHO-KI, and transfected cells containing recombinant DNA related to the DHFR gene. (bioind.com)

ED 50 = 10 - 50 ng/ml, corresponding to a specific activity of 0.02 - 0.1 x 10 6 units/mg, as determined by inhibition of apoptotic cell death induced by FASL on Jurkat cells. (biolegend.com)
IL-2 is best known for its autocrine and paracrine activity on T cells. (rndsystems.com)
90 % as determined by SDS-PAGE Activity Measured in. (biofargo.com)
The frequency and activity of terminally differentiated NKG2a/c low CD16 + natural killer cells correlates with a reduction of replication-competent SIV in lymph node during antiretroviral therapy and time to viral rebound following analytical treatment interruption. (nature.com)

75%, as determined by Coomassie stained SDS-PAGE under reducing conditions. (biolegend.com)
95%, as determined by Coomassie stained SDS-PAGE. (biolegend.com)

Other biological effects not leading to apoptotic cell death have also been described. (biolegend.com)
The effects of the chemicals in the diluent are to release metals bound to red blood cells making them available for ionization, reduce ionization suppression by the biological matrix, prevent clogging of the sample introduction system pathways by undissolved biological solids, and allow introduction of internal standards to be utilized in the analysis step. (cdc.gov)

However, linear DNA templates are rapidly degraded by exonucleases that are naturally present in the cell extracts. (jove.com)
By using cell extracts from exonuclease-deficient knockout cells, linear DNA templates remain intact without requiring any end-modifications. (jove.com)

Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation. (cusabio.com)

Unlike SCF, FLT3 ligand has no effect on mast cells. (biofargo.com)

IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells (9-11). (rndsystems.com)

Despite the existence of a highly immunosuppressive tumor environment, adenovirus-infected cells can nonetheless be efficiently cleared by infiltrating cytotoxic T lymphocytes (CTL) without compromising tumor burden. (aacrjournals.org)

Anatomy21

Organisms13

Diseases5

Chemicals and Drugs102

Analytical, Diagnostic and Therapeutic Techniques and Equipment39

Psychiatry and Psychology1

Phenomena and Processes76

Disciplines and Occupations3

Technology, Industry, Agriculture3

Information Science3

Health Care6

Geographicals1

Thaw cycles5

Proliferation11

Endothelial6

Optimal7

Antibodies3

Expressed in HEK293 cells2

Reconstitution1

Purity3

Biol2

Protein13

Dendritic cells4

Mammalian6

Binds2

HEK2933

Lysate2

Proteins7

Concentrations6

Receptor6

Macrophages2

Concentration7

Frostbite3

Antibody2

Formulation2

Reagent1

Cytokine production1

Induces2

Assays1

Tissues3

Temperatures1

Vivo3

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NHANES2

Tissue3

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Sterile1

Preparation1

PBMC1

Assay1

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Residues1

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IL101

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Tumor1