• Arachidonate 5-lipoxygenase, also known as ALOX5, 5-lipoxygenase, 5-LOX, or 5-LO, is a non-heme iron-containing enzyme (EC 1.13.11.34) that in humans is encoded by the ALOX5 gene. (wikipedia.org)
  • The ALOX5 gene, which occupies 71.9 kilobase pairs (kb) on chromosome 10 (all other human lipoxygenases are clustered together on chromosome 17), is composed of 14 exons divided by 13 introns encoding the mature 78 kilodalton (kD) ALOX5 protein consisting of 673 amino acids. (wikipedia.org)
  • ALOX5 products, particularly 5-hydroxyeicosatetraenoic acid and 5-oxo-eicosatetraenoic acid, promote the proliferation of these ALOX5 aberrantly expressing tumor cell lines suggesting that ALOX5 acts as a pro-malignancy factor for them and by extension their parent tumors. (wikipedia.org)
  • Description: Quantitative sandwich ELISA for measuring Mouse Arachidonate 5-lipoxygenase (ALOX5) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. (glideruniversity.org)
  • Description: Quantitativesandwich ELISA kit for measuring Rat Arachidonate 5-lipoxygenase (Alox5) in samples from serum, plasma, cell culture supernates, tissue homogenates. (glideruniversity.org)
  • Description: The ALOX5 gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. (glideruniversity.org)
  • Description: Quantitativesandwich ELISA kit for measuring Human Arachidonate 5-lipoxygenase (ALOX5/LOG5) in samples from serum, plasma, cell culture supernates, tissue homogenates. (glideruniversity.org)
  • Here we examined the molecular mechanism by which multi-walled CNTs (MWCNTs) induce M1 polarization in vitro, with a focus on the induction of arachidonate 5-lipoxygenase (Alox5), a key enzyme in the biosynthesis of LMs. (cdc.gov)
  • Pharmacologic inhibition of the leukotriene-generating enzyme arachidonate 5-lipoxygenase (Alox5) reduces metastasis, revealing the efficacy of using targeted therapy against a specific tumour microenvironment component ( 2 ). (irbbarcelona.org)
  • It is a medicinal herb with antidepressant activity and potent anti-inflammatory properties as an arachidonate 5-lipoxygenase inhibitor and COX-1 inhibitor. (cbaextract.com)
  • Increased cytosol Ca(2+) and type 1 programmed cell death in Bcl-2-positive U937 but not in Bcl-2-negative PC-3 and Panc-1 cells induced by the 5-lipoxygenase inhibitor MK 886. (rush.edu)
  • HKD2 and HKE2 were formed in human blood leukocytes activated with bacterial lipopolysaccharide and calcium ionophore A23187, and biosynthesis was blocked by inhibitors of 5-LOX or COX-2. (elsevierpure.com)
  • While 5α-reductase inhibitors appear to decrease low-grade cancer risk, they do not affect high-grade cancer risk, thus are not recommended for prevention. (owiki.org)
  • Included under this category are inhibitors that are specific for lipoxygenase subtypes and act to reduce the production of LEUKOTRIENES. (bvsalud.org)
  • One unit is defined as the amount of enzyme that consumes 1.0 nmol of oxygen per min at 25°C in 50 mM Tris-HCl, 100 µM arachidonate, 2 mM CaCl₂, 1 mM ATP, 250 µM oxygen. (sigmaaldrich.com)
  • An enzyme that catalyzes the oxidation of arachidonic acid to yield 12-hydroperoxyarachidonate (12-HPETE) which is itself rapidly converted by a peroxidase to 12-hydroxy-5,8,10,14-eicosatetraenoate (12-HETE). (umassmed.edu)
  • In this work, the metabolomic characterization of the ethanolic extracts of these species and the determination of the antioxidant activity, enzymatic inhibition and anti-inflammatory potential of selected compounds on the 5-lipoxygenase enzyme by molecular docking and cytoprotective activity in the SH-SY5Y cell line were carried out. (edu.pe)
  • Molecular docking evidenced significant binding affinities of some compounds for the 5-lipoxygenase enzyme, together with outstanding pharmacokinetic properties. (edu.pe)
  • Cysteinyl leukotrienes (Cys-LTs) are potent inflammatory lipid mediators derived from the 5-lypoxygenase (5-LO) pathway of arachidonic acid (AA) metabolism [ 1 ], initially identified to have important effects on pathogenetic aspects of allergic rhinitis and bronchial asthma and approved in the late 1990s for the relief of perennial and seasonal allergic rhinitis symptoms, and the treatment of mild persistent bronchial asthma [ 2 - 5 ]. (hindawi.com)
  • Enteroaggregative Escherichia coli promotes transepithelial migration of neutrophils through a conserved 12-lipoxygenase pathway. (umassmed.edu)
  • Collectively, these landmark discoveries provided initial insight into the COX pathway of arachidonate metabolism. (medscape.com)
  • The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). (glideruniversity.org)
  • Upon stimulation of the cell, 5-LO translocates to the nuclear membrane where it is found in association with 5-LO activating protein (FLAP) and with the 85 kDa cPLA 2 . (sigmaaldrich.com)
  • Both genes encode enzymes, phosphodiesterase 4D (PDE4D) and arachidonate 5-lipoxygenase-activating protein (FLAP), which suggest novel treatment strategies for stroke prevention. (rais.is)
  • HO2) residue to arachidonic acid (i.e. 5Z,8Z,11Z,14Z-eicosatetraenoic acid) at carbon 5 of its 1,4 diene group (i.e. its 5Z,8Z double bonds) to form 5(S)-hydroperoxy-6E,8Z,11Z,14Z-eicosatetraenoic acid (i.e. 5S-HpETE). (wikipedia.org)
  • 5-Lipoxygenase, Human, Recombinant, S. fruigiperda 5-Lipoxygenase, Human, Recombinant, S. frugiperda E.C 1.13.11.34, catalyzes the formation of 5(S)-HpETE from arachidonic acid and its subsequent conversion to leukotriene A4. (sigmaaldrich.com)
  • 5-LO catalyzes the formation of 5(S)-HpETE from arachidonic acid and its subsequent conversion to leukotriene A 4 . (sigmaaldrich.com)
  • 12-Lipoxygenase plays a key role in cell death caused by glutathione depletion and arachidonic acid in rat oligodendrocytes. (umassmed.edu)
  • The prostaglandin and leukotriene families of lipid mediators are formed via two distinct biosynthetic pathways that are initiated by the oxygenation of arachidonic acid by either cyclooxygenase-2 (COX-2) or 5-lipoxygenase (5-LOX), respectively. (elsevierpure.com)
  • The 5-LOX product 5S-hydroxyeicosatetraenoic acid, however, can also serve as an efficient substrate for COX-2, forming a bicyclic diendoperoxide with structural similarities to the arachidonic acid-derived prostaglandin endoperoxide PGH 2 [Schneider C, et al. (elsevierpure.com)
  • The AA, a normal component of cell membrane phospholipids, serve as substrate for prostaglandin endoperoxide (PGH) syntases-1 and -2, also known as cycloxygenase (COX)-1 and -2, lipoxygenase (5-, 12-, or 15) (LO), or cytochrome p450 enzymes [ 18 ]. (hindawi.com)
  • The crude fixed oil and pure thymoquinone both inhibited the cyclo-oxygenase and 5-lipoxygenase pathways of arachidonate metabolism in rat peritoneal leukocytes stimulated with calcium ionophore A23187, as shown by dose-dependent inhibition of thromboxane B 2 and leukotriene B 4 , respectively. (thieme-connect.com)
  • Identification of the highly oxygenated hemiketal eicosanoids provides evidence for a previously unrecognized biosynthetic cross-over of the 5-LOX and COX-2 pathways. (elsevierpure.com)
  • HKD 2 and HKE 2 stimulated migration and tubulogenesis of microvascular endothelial cells, implicating a proangiogenic role of the hemiketals in inflammatory sites that involve expression of 5-LOX and COX-2. (elsevierpure.com)
  • Because the gene page contains a lot of information, it takes on average 3-5 seconds to load. (uth.edu)
  • a GC-rich core region including the Sp1/Egr-1 sites may be critical for basal 5-LO promoter activity. (wikipedia.org)
  • Description: A competitive ELISA for quantitative measurement of Canine Arachidonate 15 lipoxygenase (ALOX15/LOG15) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (myelisakit.com)
  • Systemic disease during Streptococcus pneumoniae acute lung infection requires 12-lipoxygenase-dependent inflammation. (umassmed.edu)
  • Arachidonate 12-Lipoxygenase" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (umassmed.edu)
  • Pneumolysin Induces 12-Lipoxygenase-Dependent Neutrophil Migration during Streptococcus pneumoniae Infection. (umassmed.edu)
  • Recombinant, human 5-Lipoxygenase expressed in S. fruigiperda insect cells using a baculovirus overexpression system. (sigmaaldrich.com)
  • Growth control of lung cancer by interruption of 5-lipoxygenase-mediated growth factor signaling. (rush.edu)
  • These results suggest that the production of soluble EMMPRIN, phospholipase A(2) and 5-lipoxygenase activities are sites for potential therapeutic intervention. (birmingham.ac.uk)
  • Trends in Molecular Medicine , 11 (5), 217-224. (rais.is)
  • Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. (glideruniversity.org)