• In this study, we show that HeLa cells also express auxilin 1, previously thought to be neuron specific, and that both auxilins need to be depleted for inhibition of clathrin-mediated endocytosis and intracellular sorting. (biomedsearch.com)
  • Depleting both auxilins cause an approximately 50% reduction in the number of clathrin-coated pits at the plasma membrane but enhances the association of clathrin and adaptors with intracellular membranes. (biomedsearch.com)
  • Together, these findings indicate that the two auxilins have overlapping functions and that they not only facilitate the uncoating of CCVs but also prevent the formation of nonproductive clathrin cages in the cytosol. (biomedsearch.com)
  • Auxilins (in mammalian cells, auxilin1 (Aux1) and auxilin2 or GAK) need both a clathrin-binding area and a phosphatase and tensin homologue (PTEN)-like area for effective recruitment to recently budded clathrin-coated vesicles4,7,8 (Prolonged Data Fig. 1a, Supplementary Video 1). (chinamicrospine.com)