AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPsychiatry and PsychologyPhenomena and ProcessesDisciplines and OccupationsAnthropology, Education, Sociology and Social PhenomenaInformation ScienceNamed GroupsHealth CareGeographicals
Hematopoietic Stem Cell TransplantationTransplantation, HomologousStem Cell TransplantationGraft vs Host DiseaseTransplantation ConditioningTransplantation, AutologousPeripheral Blood Stem Cell TransplantationHematologic NeoplasmsTransplantation ChimeraStem CellsBone Marrow TransplantationGraft vs Leukemia EffectMyeloablative AgonistsTissue DonorsBusulfanGraft SurvivalTreatment OutcomeVidarabineRemission InductionRecurrenceGraft vs Tumor EffectHistocompatibility TestingLymphocyte TransfusionHematopoietic Stem CellsCord Blood Stem Cell TransplantationImmunosuppressive AgentsWhole-Body IrradiationLeukemia, Myeloid, AcuteLeukemiaMultiple MyelomaRetrospective StudiesCombined Modality TherapyLeukemia, Myelogenous, Chronic, BCR-ABL PositiveSiblingsCytomegalovirus InfectionsMinor Histocompatibility AntigensLymphocyte DepletionSalvage TherapyHistocompatibilityDisease-Free SurvivalAntilymphocyte SerumSurvival AnalysisLiver TransplantationMyelodysplastic SyndromesSurvival RateVirus ActivationNeoplasm, ResidualChimerismPrecursor Cell Lymphoblastic Leukemia-LymphomaMelphalanHematopoietic Stem Cell MobilizationMesenchymal Stem Cell TransplantationTime FactorsHepatic Veno-Occlusive DiseaseCyclophosphamideAntigens, CD34T-LymphocytesAntineoplastic Combined Chemotherapy ProtocolsCytomegalovirusCell TransplantationAnemia, AplasticAcute DiseasePrimary MyelofibrosisHLA AntigensEmbryonic Stem CellsHematologic DiseasesKidney TransplantationFollow-Up StudiesTransplantation ImmunologyImmunosuppressionLeukemia, Myeloid, Accelerated PhasePrognosisAntibodies, NeoplasmRoseolovirus InfectionsAdult Stem CellsCytarabineLeukemia, MyeloidGraft RejectionBenzamidesGranulocyte Colony-Stimulating FactorImmunocompromised HostLymphoproliferative DisordersAntineoplastic AgentsFusion Proteins, bcr-ablHodgkin DiseaseBone Marrow CellsNuclear FamilyReceptors, Purinergic P2X5PyrimidinesLymphoma, Non-HodgkinCell DifferentiationTransplantation, IsogeneicFlow CytometryInfectionGanciclovirCyclosporineLeukocyte TransfusionImmunotherapy, AdoptivePiperazinesHeart Transplantation
Hematopoietic Cell TransplantationHSCTHaematopoieticGraftDonorClinicalAcuteAutologous and allogeneicMSCsTherapiesPluripotentHandling of hematopoietic stemNeutrophilsProgenitorTherapyStromalDiseasesTissuePatientsDonorsDifferentiationResidualImmuneMarrowPeripheral bloodChronic lymphocyDisordersResponsesLymphocyteTranslational medicineOrgansMesenchymal cellViralTransductionPlateletsUmbilicalPathogenicGeneManifestationsGvHDLungBloodInfections
Hematopoietic Cell Transplantation4
  • Real-World Data Showing Trends and Outcomes by Race and Ethnicity in Allogeneic Hematopoietic Cell Transplantation: A Report from the Center for International Blood and Marrow Transplant Research. (mcw.edu)
  • The first-ever online and open-access education e-course, brought to you by The EBMT Trainee Committee, designed to cover all aspects surrounding hematopoietic cell transplantation and cellular therapy with a focus on trainee-relevant knowledge and skills. (ebmt.org)
  • The specific medications administered depend on the choice of therapy and whether it is supportive care only, immunosuppressive therapy, or hematopoietic cell transplantation. (medscape.com)
  • Central venous catheter placement is required before the administration of hematopoietic cell transplantation. (medscape.com)
HSCT7
Haematopoietic4
  • Independent origins of fetal liver haematopoietic stem and progenitor cells. (u-tokyo-hemat.com)
  • CCDC88C-FLT3 gene fusion in CD34-positive haematopoietic stem and multilineage cells in myeloid/lymphoid neoplasm with eosinophilia. (u-tokyo-hemat.com)
  • After undergoing an allogeneic haematopoietic stem cell transplantation (alloHCT), patients need intensive physiological and psychological rehabilitation. (mdpi.com)
  • Identifying and understanding acute complications after allogeneic haematopoietic cell transplantation (alloHCT). (ebmt.org)
Graft7
  • White blood cells of the donor's immune system which remain within the donated tissue (the graft) recognize the recipient (the host) as foreign (non-self). (wikipedia.org)
  • Solid tumors, autoimmune diseases, graft versus host disease (GvHD), genetic diseases and neurodegenerative diseases are investigated using many types of autologous and allogeneic cells with rapidly evolving cell engineering and editing approaches. (bdbiosciences.com)
  • This includes bone and cartilage repair, cell types into which MSCs readily differentiate, and immune conditions such as graft versus host disease and autoimmune conditions that utilize the MSC's immune suppressive properties. (biomedcentral.com)
  • Graft-versus-host disease (GVHD) is one of the major obstacles for the success of allogeneic hematopoietic stem cell transplantation. (aai.org)
  • It could also be due to supporting cells in the graft, which are normally excluded when CD34 + cells are directly enriched. (fredhutch.org)
  • It is used for the prophylaxis of graft rejection in organ and tissue transplantation. (lookformedical.com)
  • or actively by prior immunization of the recipient with graft antigens which evoke specific antibodies and form antigen-antibody complexes which bind to the antigen receptor sites of the T-cells and block their cytotoxic activity. (lookformedical.com)
Donor13
  • Engraftment of donor-type MSC-derived osteoblasts was shown using bone specimens and microsatellite DNA marker analysis. (issca.us)
  • Researchers and clinicians have long sought a method to replace the β cells, but these efforts have been hindered by the difficulty in developing a reliable and sustainable donor b cell source and a way to engraft them that avoids both immune-mediated allogeneic rejection and continuing autoimmune b cell destruction. (jax.org)
  • Researchers have attempted encapsulating donor β cells in a variety of materials to prevent allo- and xenogeneic rejection, but these materials themselves can stimulate innate immune responses. (jax.org)
  • Impact of Donor Age in Haploidentical-Post-Transplantation Cyclophosphamide versus Matched Unrelated Donor Post-Transplantation Cyclophosphamide Hematopoietic Stem Cell Transplantation in Patients with Acute Myeloid Leukemia. (mcw.edu)
  • Unrelated Donor Cord Blood Transplantation in Children: Lessons Learned Over 3 Decades. (mcw.edu)
  • However, HLA-identical sibling stem cell infusions in ADA-SCID result in some donor stem cell engraftment and long-term thymopoiesis. (frontiersin.org)
  • She received a matched sibling donor unconditioned stem cell infusion at 16 months of age. (frontiersin.org)
  • There was 93% donor T-lymphocytes, 20% donor B-lymphocytes, and 5% donor myeloid cells, indicative of some donor stem cell engraftment. (frontiersin.org)
  • This pilot clinical trial studies the side effects of irradiated donor cells following stem cell transplant in controlling cancer in patients with hematologic malignancies. (bioseek.eu)
  • Transfusion of irradiated donor cells (immune cells) from relatives may cause the patient's cancer to decrease in size and may help control cancer in patients receiving a stem cell transplant. (bioseek.eu)
  • When tested on healthy donor bone marrow cells, the lineage depleted cells and CD34 + -enriched cells were transduced with similar efficiencies by the FANCA-carrying viral vector. (fredhutch.org)
  • In the setting of allogeneic hematopoietic stem cell transplantation, donor lymphocyte infusions are frequently given to treat relapse of hematologic malignancies. (ashpublications.org)
  • An induced state of non-reactivity to grafted tissue from a donor organism that would ordinarily trigger a cell-mediated or humoral immune response. (lookformedical.com)
Clinical23
  • Later, Arnold Caplan's group described mesenchymal stem/stromal cells (MSCs) as multipotent mesenchymal cell populations which can differentiate into several tissue types, and demonstrated roles for MSCs in the regeneration of bone, cartilage or ligaments in animal and clinical studies [2-4]. (issca.us)
  • Blood and Marrow Transplant Research [CIBMTR] in 2004), the European Research Project on Cord Blood Transplantation (Eurocord) in 1993, and the Japanese Cord Blood Banking Network in 1996-expedited the clinical evaluation of the efficacy and safety of transplantation of cord blood from unrelated donors. (nationalacademies.org)
  • Building off experience in hematopoietic stem cell transplant and exploiting advances in genetic engineering, genome editing, immunology, immunotherapy, stem cell biology, understanding disease mechanisms and cell therapy manufacturing, considerable progress has been made with hundreds of clinical trials underway. (bdbiosciences.com)
  • The technique has also shown preliminarily promising outcomes in B cell acute lymphoblastic leukemia (B-ALL), B cell non-Hodgkin's lymphoma (B-NHL), chronic lymphocytic leukemia (CLL), Hodgkin's lymphoma and multiple myeloma in clinical trials targeting CD19, CD20, CD22, CD30 and B-cell maturation antigen (BCMA), with many more under investigation. (bdbiosciences.com)
  • T cell-targeted immunomodulators such as monoclonal antibodies against PD-1 or CTLA4 may be used in combination with CAR-T cells in clinical trials. (bdbiosciences.com)
  • In recent years, clinical trials with stem cells have taken the emerging field in many new directions. (biomedcentral.com)
  • The rapid advance of stem cell clinical trials for a broad spectrum of conditions warrants an update of the review by Trounson (2009) [ 1 ]. (biomedcentral.com)
  • There has been a rapid surge in clinical trials involving stem cell therapies over the last two to three years and those trials are establishing the clinical pathways for an emergent new medicine. (biomedcentral.com)
  • Placenta-derived stem cells are being considered for similar uses and are in Phase III clinical trial for critical limb ischemia by Israel's Pluristem Therapeutics. (biomedcentral.com)
  • A significant proportion of clinical studies that are underway involve bone marrow and cord blood stem cells for blood and immune disorders [ 3 ] and cancers. (biomedcentral.com)
  • We have chosen to concentrate on the emerging therapeutics that broadly involves a wide range of cell types in clinical trials registered on the National Institutes of Health's clinical trials web site. (biomedcentral.com)
  • Engraftment, clinical, and molecular follow-up of patients with multiple myeloma who were reinfused with highly purified CD34+ cells to support single or tandem high-dose chemotherapy. (shengsci.com)
  • Eighty-two patients with advanced multiple myeloma (MM) were enrolled in 2 sequential clinical studies of 1 or 2 courses of myeloablative therapy with stem cell support. (shengsci.com)
  • T-cell responses correlate directly with clinical symptoms, and were better predictors of HHV-6 disease than viral load or total CD3+ counts. (hhv-6foundation.org)
  • Because of this alternative use of MSCs, Caplan and Sorell [ 14 ] suggested a renaming of MSCs to medicinal signaling cells to suggest a new era of MSC clinical relevance due to their immunomodulatory properties. (biomedcentral.com)
  • In contrast, patients with self-reactive cells have significant autoimmune features in addition to their clinical and molecular immunodeficient state. (hindawi.com)
  • Familiarize trainees with important clinical aspects of stem cell transplant and cellular therapy, including pharmacology, patient monitoring, and recognition and basic management of complications. (ebmt.org)
  • RQR8 is a 136-amino-acid epitope-based marker/suicide gene that enables clinical selection, cell tracking, and deletion in case of toxicity. (ashpublications.org)
  • A compact marker/suicide gene that utilizes established clinical-grade reagents and pharmaceuticals would be of considerable practical utility to T-cell cancer gene therapy. (ashpublications.org)
  • The clinical presentation of patients with aplastic anemia includes signs and symptoms related to the decrease in bone marrow production of hematopoietic cells. (medscape.com)
  • Clinical and laboratory observations suggest that acquired aplastic anemia is an autoimmune disease. (medscape.com)
  • Areas related to clinical and experimental transplantation are also of interest. (lidsen.com)
  • Cytomegalovirus (CMV) is a beta variant of the Herpes Simplex Virus (HSV), a highly prevalent opportunistic pathogen with over 50% of adults infected worldwide by age [ 1. De Matos SB, Meyer R, de Mendonça Lima FW. Cytomegalovirus infection after renal transplantation: Occurrence, clinical features, and the cutoff for antigenemia in a university hospital in Brazil. Infect Chemother. 2017; 49: 255-261. [ CrossRef ] ">1 ]. (lidsen.com)
Acute10
  • About one-third to one-half of allogeneic transplant recipients will develop acute GvHD. (wikipedia.org)
  • CAR-T cells, which incorporate an antibody-derived extracellular receptor and T cell derived intracellular signaling domains, have shown convincing outcomes in certain types of leukemia and lymphoma, including commercial licensure of CD19 CAR-Ts for the treatment of relapsed/ refractory large B cell lymphoma and acute lymphoblastic leukemia. (bdbiosciences.com)
  • In April 1960, Dr. Álvaro Gómez-Leal, presented during the first meeting of the Agrupación Mexicana para el Estudio de la Hematología, A.C., data on a transplant of allogeneic stem cells in a patient with acute leukemia done in Monterrey, Mexico: the patient received high-dose chemotherapy followed by stem cells from the bone marrow of his brother, improving and obtaining remission for months but relapsing and subsequently died. (revistadehematologia.org.mx)
  • Measurable Residual IDH1 before Allogeneic Transplant for Acute Myeloid Leukemia. (mcw.edu)
  • DNA Sequencing to Detect Residual Disease in Adults With Acute Myeloid Leukemia Prior to Hematopoietic Cell Transplant. (mcw.edu)
  • Preemptive immunotherapy in childhood acute myeloid leukemia for patients showing evidence of mixed chimerism after allogeneic stem cell transplantation. (shengsci.com)
  • Previous studies have shown that children with acute myeloid leukemia (AML) who developed mixed chimerism (MC) were at high risk for relapse after allogeneic stem-cell transplantation (allo-SCT). (shengsci.com)
  • I have expertise in novel therapies for acute myeloid leukemia, stem cell mobilization and homing, as well as stem cell transplantation. (rochester.edu)
  • Autologous bone marrow transplantation for acute leukemia: Results and prognostic factors in 90 consecutive patients. (cellsilab.com)
  • In this article, we report that the interaction between OX40L and OX40 is of critical importance for both induction and progression of acute GVHD (aGVHD) driven by human T cells. (aai.org)
Autologous and allogeneic1
  • Pain management, osteochondral, nerve, or blood vessel support by MSCs derived from both autologous and allogeneic sources have been examined. (biomedcentral.com)
MSCs24
  • Mesenchymal stem/stromal cells (MSCs) are increasingly used as an intravenously applied cellular therapeutic. (issca.us)
  • Pivotal studies by the group of Horwitz in children with osteogenesis imperfecta, an inherited enzyme deficiency of collagen synthesis by mesenchymal cells in bone, opened the field for intravenous use of MSCs. (issca.us)
  • Therefore, the authors concluded that transplantation of isolated healthy allogeneic MSCs might cure the disease. (issca.us)
  • The tissue source of the MSCs was in most cases not decisive, and cells from various tissue sources were explored. (issca.us)
  • and signals from the injected cells were found early after administration of the MSCs at the highest frequencies in the lungs, followed by liver and spleen. (issca.us)
  • There are many studies involving autologous therapies and some allogenic therapies, based on the recovery of mobilized bone marrow cells, including mesenchymal stem cells (MSCs) and adipose derived stem cells that also include the stromal or adherent cell type that has an MSC phenotype. (biomedcentral.com)
  • The use of patient's own bone marrow aspirates, hematopoietic stem cells and MSCs, for heart muscle tissue repair can be puzzling because these cells do not normally contribute to the cardiac lineage types that are desired. (biomedcentral.com)
  • Here, we used phenotypically uniform trophoblast stage-derived mesenchymal stem cells (T-MSCs) from embryonic stem cells, which are capable of stable production, and their exosomes (T-MSCs-Exo) to explore the molecular mechanisms involved in dopaminergic (DA) neuron protection in PD models using experimental assays (e.g., western blotting, immunofluorescence and immunohistochemistry staining). (biomedcentral.com)
  • We assessed the levels of DA neuron injury and oxidative stress in MPTP-induced PD mice and MPP + -induced MN9D cells after treating them with T-MSCs or T-MSCs-Exo. (biomedcentral.com)
  • Similarly, intranasal administration of umbilical cord mesenchymal stem cells (UC-MSCs) significantly alleviated the locomotor deficits and rescued DA neurons in MPTP-induced PD mice by cross-talk between the brain and gut [ 12 ]. (biomedcentral.com)
  • Adult mesenchymal stem cells (MSCs) represent a subject of intense experimental and biomedical interest. (biomedcentral.com)
  • MSCs can be defined by their ability to adhere to tissue culture plastic, their expression of several cell surface molecular epitopes-cluster of differentiation CD73, CD90, and CD105, and others-as well as their lack of several surface markers, including CD45 [ 6 ]. (biomedcentral.com)
  • Mesenchymal stem cells (MSCs) have been suggested as an appropriate source for diabetes cell-based therapies. (biomedcentral.com)
  • Flow cytometry analysis and three-lineage differentiation ability demonstrated that fibroblast-like cells obtained from primary culture had MSCs' characteristics. (biomedcentral.com)
  • Mesenchymal stem cells (MSCs) have received more attention due to their proliferation and differentiation capacity and low immunogenicity. (biomedcentral.com)
  • Cell-based therapy utilizing mesenchymal stromal cells (MSCs) is an attractive option for augmenting the fracture repair process. (hindawi.com)
  • Osteoprogenitor MSCs not only differentiate into bone, but they also exert modulatory effects on immune cells via a variety of mechanisms. (hindawi.com)
  • In this paper, we review the current literature on both in vitro and in vivo studies on the role of the immune system in fracture repair, the use of MSCs in the enhancement of fracture healing, and interactions between MSCs and immune cells. (hindawi.com)
  • The normal process of fracture repair begins with an immediate inflammatory response as the innate immune system (macrophages, monocytes, neutrophils, and NK cells) responds with a variety of cytokines that recruit and activate several cell types, including osteoprogenitor mesenchymal stem cells (MSCs), to the site of injury [ 1 , 2 ]. (hindawi.com)
  • Multipotent mesenchymal stromal cells (MSCs), also known as mesenchymal stem cells, have the capacity to differentiate into a variety of cell types (Figure 1 ), including adipocytes, chondrocytes, and osteocytes [ 9 , 10 ]. (hindawi.com)
  • Coupled with reports that allogeneic MSCs have immunoprivileged status and immunomodulatory properties, there has been considerable interest in exploring the use of these cells as a therapeutic option for bone repair. (hindawi.com)
  • Adipose-derived MSCs, in particular, pose an attractive option for cell-based therapy due to their relatively decreased morbidity during isolation and potential for expansion and differentiation [ 12 ]. (hindawi.com)
  • Multipotential differentiation of MSCs into adipogenic, osteogenic, and chondrogenic cell lineages. (hindawi.com)
  • MSCs are able to evade the host cell immune system due to their low expression of major histocompatibility complex (MHC) class I molecules and complete lack MHC class II molecules and other costimulatory molecules (CD40, CD40L, CD80, and CD86) required for immune cell stimulation [ 15 - 17 ]. (hindawi.com)
Therapies6
  • Gene and cell therapies present novel alternatives to disease management, offering the promise of a single treatment and a lifelong cure. (bvsalud.org)
  • Engineered chimeric antigen receptor (CAR) T cell therapies (CAR-T) are now commercially available to treat certain leukemias and lymphomas. (bdbiosciences.com)
  • Investigational cell therapies are addressing a wide range of hematological malignancies. (bdbiosciences.com)
  • From a research, medical, and business standpoint, mesenchymal stem cell (MSC)-based therapies are fascinating. (biomedcentral.com)
  • In recent decades, stem cell-based therapies have raised hopes for treating incurable diseases such as diabetes [ 3 ]. (biomedcentral.com)
  • Current therapies for NSCLC including chemotherapy regimens and drugs that target molecular changes in cancer cells have considerable downsides. (biomedfrontiers.org)
Pluripotent4
  • The proliferation and differentiation of pluripotent stem cells give rise to progeny that can populate the entire immunologic and hematopoietic systems through committed progenitors of both the lymphoid and myeloid lineages. (medscape.com)
  • a multi-institutional team lead by Dr. Daniel G. Anderson of the Massachusetts Institute of Technology and Boston Children's Hospital describes a new approach in which β cells differentiated in vitro from human pluripotent stem cells (hPSCs) were packaged in a modified alginate matrix and transplanted into immuno-competent C57BL/6J mice ( 000664 ) made diabetic by treatment with streptozotocin (STZ), which selectively destroys the mouse b cells. (jax.org)
  • At the same time, the first three trials using cells derived from pluripotent cells have begun. (biomedcentral.com)
  • 2015年11月11日, the TTS-JST Transplantation Science Mentee-Mentor Travel Award, International Basic Science Mentee-Mentor Awards-TSS2015, Propagated NK cells from human pluripotent stem cells exert vigorous cytotoxicity against HCC and inhibit HCV replication. (hiroshima-u.ac.jp)
Handling of hematopoietic stem1
Neutrophils2
  • Consistently fewer macrophages, neutrophils, β cells, and cytotoxic T cells were associated with TMTD alginate spheres compared to unmodified alginate spheres. (jax.org)
  • Patient PBMCs are collected by leukapheresis followed by enrichment approaches to isolate T cells while removing other cells such as B cells, monocytes, neutrophils, dendritic cells and contaminating tumor cells. (bdbiosciences.com)
Progenitor10
  • The etiology of bone marrow failure (BMF) includes defective stem/progenitor cells and/or stroma/accessory cells/growth factors, as well as deficient nonspecific nutrients or, as in the case of acquired aplastic anemia, immune-mediated abnormalities. (medscape.com)
  • Inherited bone marrow failure syndromes (IBMFS) are usually the result of intrinsic stem cell/progenitor defects. (medscape.com)
  • B lood cell differentiation begins with multipotent hematopoietic progenitor cells (HPCs), which are located in the marrow spaces of the bone. (nationalacademies.org)
  • As the cells reproduce, they commit to a particular task or cell line and become known as committed progenitor cells . (nationalacademies.org)
  • These committed progenitor cells are difficult to discern from the original multipotent cells but can be cultured to form colonies of specific types of blood cells (Guyton and Hall, 2000). (nationalacademies.org)
  • Umbilical cord blood is a rich source of these committed progenitor cells and, presumably, multipotent HPCs (Knudtzon, 1974). (nationalacademies.org)
  • Enhancement of human erythroid progenitor cell growth by media conditioned by a human t-lymphocyte line. (shengsci.com)
  • Fanconi anemia (FA) is an inherited disorder affecting DNA repair in hematopoietic (blood) stem and progenitor cells (HSPCs) from the bone marrow. (fredhutch.org)
  • Spontaneous In Vivo Chondrogenesis of Bone Marrow-Derived Mesenchymal Progenitor Cells by Blocking Vascular Endothelial Growth Factor Signaling. (unibas.ch)
  • Engineering Small-Scale and Scaffold-Based Bone Organs via Endochondral Ossification Using Adult Progenitor Cells. (unibas.ch)
Therapy15
  • Tremendous advances have been made in cell therapy in the past decade. (bdbiosciences.com)
  • A common theme in cell therapy is the need to characterize and understand cell phenotypes and heterogeneity at every step of the process. (bdbiosciences.com)
  • Cell Therapy Informatics: Updates on the Integration of HCT/IEC Functionalities into an Electronic Medical Record System in the US to Promote Efficiency, Patient Safety, Research, and Data Interoperability. (mcw.edu)
  • Harmonizing Definitions for Diagnostic Criteria and Prognostic Assessment of Transplantation-Associated Thrombotic Microangiopathy: A Report on Behalf of the European Society for Blood and Marrow Transplantation, American Society for Transplantation and Cellular Therapy, Asia-Pacific Blood and Marrow Transplantation Group, and Center for International Blood and Marrow Transplant Research. (mcw.edu)
  • The only curative therapy is hematopoietic stem cell transplantation. (frontiersin.org)
  • Could some complications of CAR-T cell therapy be secondary to HHV-6 infection? (hhv-6foundation.org)
  • Might HHV-6 contribute to some cases of cytokine release syndrome, pneumonia, and cytopenias in CAR-T Cell therapy? (hhv-6foundation.org)
  • An experienced multidisciplinary team in close consultation with the primary transplantation centre should perform the rehabilitation therapy. (mdpi.com)
  • Bone marrow transplantation coupled with gene therapy is a potential treatment, however, the paucity of available HSPCs and their sensitivity to ex vivo manipulation complicate the approach. (fredhutch.org)
  • To improve isolation and modification of HSPCs as well as their engraftment in the transplant recipients, the authors modified recommendations from the international FA Gene Therapy Working Group. (fredhutch.org)
  • The international FA Gene Therapy Working Group recommends direct CD34+ cells enrichment using beads binding to the CD34 marker. (fredhutch.org)
  • This could re-open the door for up-and-coming, non-chemotherapy based conditioning regimens currently under study to improve the therapeutic potential of gene therapy for the many FA patients who do not have suitable allogeneic donors available. (fredhutch.org)
  • Hematopoietic stem cell transplantation is the only potentially curative therapy, but it is only an option for select patients. (cancernetwork.com)
  • We predict that RQR8 will make T-cell gene therapy both safer and cheaper. (ashpublications.org)
  • Genetic engineering of T cells greatly increases the scope and potency of T-cell therapy: T-cell receptor (TCR) transfer allows targeting of intracellular cancer antigens, whereas chimeric antigen receptors (CARs) allow targeting of surface cancer or lineage-specific antigens. (ashpublications.org)
Stromal5
  • This concept started from the observation that bone marrow transplantation can provide stromal cells able to synthesize intact collagen type I, replacing deficient patient cell function and ameliorating disease symptoms [5]. (issca.us)
  • Effects of hematopoietic stem cell adhesion on marrow stromal cell cytokine p. (rochester.edu)
  • MSC = multipotent mesenchymal stromal cell. (hindawi.com)
  • Fat-Derived Stromal Vascular Fraction Cells Enhance the Bone-Forming Capacity of Devitalized Engineered Hypertrophic Cartilage Matrix. (unibas.ch)
  • Generation of a Bone Organ by Human Adipose-Derived Stromal Cells Through Endochondral Ossification. (unibas.ch)
Diseases5
  • Chronic Granulomatous Disease , autoimmune diseases Autoimmune diseases Disorders that are characterized by the production of antibodies that react with host tissues or immune effector cells that are autoreactive to endogenous peptides. (lecturio.com)
  • 1957). This pioneering work laid the foundation for the exploration of hematopoietic stem cells and their role in treating diseases of the blood and immune system. (revistadehematologia.org.mx)
  • p>A blood and bone marrow transplant takes unhealthy stem cells out of the bone marrow and replaces them with healthy stem cells, with the intent to treat childhood cancers and nonmalignant blood and bone marrow diseases. (nemours.org)
  • More research teams are accelerating the use of other types of adult stem cells, in particular neural stem cells for diseases where beneficial outcome could result from either in-lineage cell replacement or extracellular factors. (biomedcentral.com)
  • Mesenchymal stem cells have been used to treat several diseases, but are not readily available. (biomedcentral.com)
Tissue9
  • The white blood cells present within the transplanted tissue then attack the recipient's body's cells, which leads to GvHD. (wikipedia.org)
  • Systemic sclerosis (SSc) is a systemic disease characterized by autoimmune responses, vasculopathy and tissue fibrosis. (mdpi.com)
  • The work of Jean Dausset, whose discovery of the human leukocyte antigen (HLA) system revolutionized our understanding of tissue compatibility for transplantation (Dausset J, 1958). (revistadehematologia.org.mx)
  • These early trials are showing roles for stem cells both in replacing damaged tissue as well as in providing extracellular factors that can promote endogenous cellular salvage and replenishment. (biomedcentral.com)
  • The response of target cells is further regulated by their microenvironment, involving the extracellular matrix, which may be modified by MSC-produced matrix metalloproteinases (MMPs) and tissue inhibitor of MMPs. (biomedcentral.com)
  • These cells expand in the periphery, causing tissue infiltration and damage due to breakdown of both central (e.g., autoimmune regulator, AIRE protein dysfunction) and peripheral (FOXP3 + deficiency) tolerance mechanisms [ 3 ]. (hindawi.com)
  • Low-power view of hematoxylin-eosin-stained bone marrow showing hypocellularity, with increased adipose tissue and decreased hematopoietic cells in the marrow space. (medscape.com)
  • An immune response with both cellular and humoral components, directed against an allogeneic transplant, whose tissue antigens are not compatible with those of the recipient. (lookformedical.com)
  • Low power, H and E showing a hypocellular bone marrow with increased adipose tissue and decreased hematopoietic cells in the marrow space. (medscape.com)
Patients19
  • Prior attempts to transplant β cells to restore glycemic control to T1D patients have used cadaveric- and porcine-derived cells. (jax.org)
  • Dr. George Mathé explored the transplantation of bone marrow cells from healthy donors to treat patients accidentally irradiated at high dose, expanding the possibilities of this life-saving technique (Mathé et al. (revistadehematologia.org.mx)
  • Correction to: Impact of pre-transplant induction and consolidation cycles on AML allogeneic transplant outcomes: a CIBMTR analysis in 3113AML patients. (mcw.edu)
  • Between February 1986 and March 1990, 56 patients with relapsed Hodgkin's disease treated with high-dose cyclophosphamide, carmustine, and etoposide (CBV) received an autologous peripheral stem cell transplantation (PSCT) rather than an autologous bone marrow transplantation (ABMT) because each patient had a marrow abnormality, either hypocellularity or tumor involvement. (shengsci.com)
  • I. To determine the toxicity associated with the administration of irradiated haploidentical cells (IHC) to patients with high-risk hematologic malignancies. (bioseek.eu)
  • Patients with severe combined immunodeficiency (SCID) may present with residual circulating T cells. (hindawi.com)
  • The latter includes patients with autoreactive clonal expanded T cell and patients with alloreactive transplacentally maternal T cells. (hindawi.com)
  • MHC-II deficient patients had slightly reduced T-cell function, normal TRECs, TCR repertoires, and normal Tregs enumeration. (hindawi.com)
  • In contrast, patients with reactive T cells exhibited poor T-cell differentiation and activity. (hindawi.com)
  • SCID patients presenting with circulating T cells show different patterns of T-cell activity and regulatory T cells enumeration that dictates the immunodeficient and autoimmune manifestations. (hindawi.com)
  • therefore patients typically do not display significant autoimmune phenomena. (hindawi.com)
  • Moreover, while the mainstay of the diagnosis of MHC-II deficiency is the absence of constitutive and inducible expression of MHC-II molecules on all cell types, other tests for T-cell function are less informative in such patients. (hindawi.com)
  • The origin of the reactive cells in such patients are either thymic release of T-cells that expand at the periphery or transplacentally transfers of maternal T lymphocytes. (hindawi.com)
  • A distinctive feature of SCID patients, which sometimes can clinically resemble Omenn, [ 6 ] is the presence of alloreactive cells originated from transplacentally maternal T lymphocytes. (hindawi.com)
  • As a consequence, blood cell counts are very low in patients and the immune system is severely weakened. (fredhutch.org)
  • However, the low frequency of CD34 + cells in FA patients limits the numbers of HSPCs that can be obtained by direct enrichment of CD34 + cells from the bone marrow. (fredhutch.org)
  • The purity of the CD34 + cells was higher than for the first two patients who underwent direct CD34 enrichment, and the transduction efficiency was similar. (fredhutch.org)
  • When asked about future directions, Dr. Adair concluded: "Of course we are interested to see whether the enhanced engraftment observed in mice plays out in our patients. (fredhutch.org)
  • We hope that this study will demonstrate the ability to not only isolate more of this limited stem cell pool from FA patients, but will also render these fewer cells more fit for engraftment. (fredhutch.org)
Donors1
Differentiation5
  • These primitive cells undergo division and differentiation to form the various peripheral blood cells. (nationalacademies.org)
  • Thioredoxin-1 regulates self-renewal and differentiation of murine hematopoietic stem cells through p53 tumor suppressor. (dukecancerinstitute.org)
  • While acknowledging progress in the other areas mentioned, this work will focus on the current debates concerning sourcing, MSC alterations of angiogenesis, cell differentiation/stimulation, and strategies to improve MSC differentiation. (biomedcentral.com)
  • For example, Omenn syndrome, a typical case of impaired T-cell differentiation with abnormal self-reactive cells, is invariably characterized by autoimmune features such as generalized scaly exudative erythroderma, enlarged lymphoid tissues, and peripheral expansion of oligoclonal T-cells, in addition to increased susceptibility for severe infections [ 3 , 4 ]. (hindawi.com)
  • A side-by-side comparison was made to evaluate the effect of each serum on proliferation rate, cell cycle, senescence, multi-lineage differentiation capacity, immunophenotype, and tumorigenesis of FPMSCs. (biomedcentral.com)
Residual3
  • Here we compared T-cell functions including the number of circulating CD3 + T cells, in vitro responses to mitogens, T-cell receptor (TCR) repertoire, TCR excision circles (TREC) levels, and regulatory T cells (Tregs) enumeration in several immunodeficinecy subtypes, clinically presenting with nonreactive residual cells (MHC-II deficiency) or reactive cells. (hindawi.com)
  • In some cases, T cells may present, as a result of residual autologous cells or transplacentally acquired maternal lymphocytes [ 1 ]. (hindawi.com)
  • Residual autologous T cells are usually emerging from partial thymic maturation impairment such as in the case of Major histocompatibility complex class II (MHC-II) deficiency. (hindawi.com)
Immune13
  • GvHD occurs when the donor's immune system's white blood cells reject the recipient. (wikipedia.org)
  • Following engraftment, the encapsulated human cells escaped xenogeneic immune destruction and maintained stable long-term glycemic control for up to six months. (jax.org)
  • These cells typically do not maintain long-term glycemic control because they stimulate strong host-mediated immune reactions leading to cell necrosis and fibrosis. (jax.org)
  • In a separate experiment, SC-β cell-loaded unmodified and TMTD-modified alginate microspheres were isolated from engrafted mice 14 days post-engraftment, and the presence of host immune cells adhering to the microspheres was examined by flow cytometry. (jax.org)
  • The smallest (0.5 mm) unmodified spheres showed the greatest number of associated immune cells. (jax.org)
  • Together, these data demonstrate that the TMTD-modified, larger microspheres induced significantly less innate immune cell stimulation and reduced fibrosis. (jax.org)
  • At the time they performed the first transplants surprisingly little was known about hematopoietic stem cells, immune responses to transplants or the complex human leucocyte antigen system. (revistadehematologia.org.mx)
  • p>In addition to childhood cancers and blood disorders, Nemours treats nonmalignant bone marrow disorders, immune system deficiencies and some metabolic disorders with allogeneic blood and bone marrow transplantation. (nemours.org)
  • While numerous teams continue to refine and expand the role of bone marrow and cord blood stem cells for their vanguard uses in blood and immune disorders, many others are looking to expand the uses of the various types of stem cells found in bone marrow and cord blood, in particular mesenchymal stem cells, to uses beyond those that could be corrected by replacing cells in their own lineage. (biomedcentral.com)
  • Transduction of CLL cells by CD40 ligand enhances an antigen-specific immune recognition by autologous T cells. (shengsci.com)
  • I. To determine if treatment with the irradiated cells induces an immune response targeting tumor associated epitopes. (bioseek.eu)
  • Immune reconstitution in severe combined immunodeficiency disease following lectin-treated T-cell depleted haplocompatible bone marrow transplantation. (cellsilab.com)
  • The precise spatial and temporal impact of immune cells and their cytokines on fracture healing remains obscure. (hindawi.com)
Marrow14
  • In the 1970s, Friedenstein and colleagues [1] first reported that locally applied culture-expanded populations of bone marrow stroma-derived fibroblastic cells remained at their injection sites under the kidney capsule, where an ectopic hematopoiesis was initiated. (issca.us)
  • Moreover, children who received transplants approached growth curves similar to the children transplanted with allogeneic complete bone marrow [6]. (issca.us)
  • GvHD is commonly associated with bone marrow transplants and stem cell transplants. (wikipedia.org)
  • New blood cells (called "stem cells") begin in the bone marrow - the red spongy material found inside the bones - and are released to the bloodstream when mature. (nemours.org)
  • Interest of study of lymphocyte phosphoglucomutase (PGM) and adenylatokinase (AK) in the early demonstration of Bone Marrow Engraftment. (cellsilab.com)
  • Mechanisms involved in the development of tolerance after haplocompatible T cell depleted bone marrow transplantation. (cellsilab.com)
  • Anti-Tetanus Toxoid Antibody Production after Mismatched T Cell Depleted Bone Marrow Transplantation. (cellsilab.com)
  • Cutaneous localization in multiple myeloma in the context of bortezomib-based treatment: how do myeloma cells escape from the bone marrow to the skin? (asmemilano.it)
  • To limit loss of CD34 + cells, the procedure was modified to remove T and B lymphocytes, monocytes and granulocytes from bone marrow or mobilized peripheral blood leukapharesis cell products (ie. (fredhutch.org)
  • Bone marrow or mobilized leukapharesis can be used as sources of CD34+ hematopoietic stem cells. (fredhutch.org)
  • Extramedullary hematopoiesis (EMH) is likely the result of abnormal trafficking of hematopoietic stem cells (HSC) from the bone marrow to organs such as the spleen, liver, and lung, causing organomegaly and sometimes organ dysfunction. (cancernetwork.com)
  • The theoretical basis for marrow failure includes primary defects in or damage to the stem cell or the marrow microenvironment. (medscape.com)
  • External insults (eg, infections, radiation, drugs) may disrupt stem cell homeostasis in marrow environment, leading to altered growth. (medscape.com)
  • Below, you will find answers to frequently asked questions about DKMS, bone marrow and blood stem cell donation, transplants, and fundraising efforts. (dkms.org)
Peripheral blood8
  • FIGURE 2-1 Formation of the multiple peripheral blood cells from multipotent hematopoietic stem cells. (nationalacademies.org)
  • 1989). Hematopoietic stem cells could be harvested easier from peripheral blood after mobilization with G-CSF (Sheridan et al. (revistadehematologia.org.mx)
  • At least 6.5 x 10(8) [corrected] mononuclear cells/kg patient weight were collected from the peripheral blood of each patient, cyropreserved, and returned intravenously following CBV administration. (shengsci.com)
  • Recent studies have shown that soluble factors elaborated by human T lymphocytes enhance erythroid burst formation by human peripheral blood null cells. (shengsci.com)
  • Automatic classification of abnormal peripheral blood cells. (cellsilab.com)
  • X-VIVO Serum-free Hematopoietic Cell Media provide nutritionally complete and balanced environments for a variety of cells including lymphokine activated killer (LAK) cells, peripheral blood lymphocytes (PBL), and tumor infiltrating lymphocytes (TIL).These media do not contain any exogenous growth factors, artificial stimulators of cellular proliferation, or undefined supplements. (lonza.com)
  • It also supports the proliferation of purified CD3+ cells isolated from peripheral blood and human tumors, and can be used to support the growth of human monocytes, macrophage cells and cell lines, PBL, granulocytes, and natural killer (NK) cells as well as the expansion of HUT-78 and related human lymphocytic cell lines. (lonza.com)
  • X-VIVO 20 - optimized to support the generation of lymphokine activated killer (LAK) cells from monocyte depleted peripheral blood lymphocytes (PBL) at high density and has also been used as a growth medium for PBL and tumor infiltrating lymphocytes (TIL). (lonza.com)
Chronic lymphocy1
  • A non-invasive approach to monitor chronic lymphocytic leukemia engraftment in a xenograft mouse model using ultra-small superparamagnetic iron oxide-magnetic resonance imaging (USPIO-MRI). (asmemilano.it)
Disorders3
  • The precise pathophysiology of inherited single cell and multilineage cytopenias has not been elucidated despite the identification of many of the genes mutated in these disorders. (medscape.com)
  • 1992). Since then, hematopoietic stem cells, with their unique ability to differentiate into various blood cell types, have proven to be a revolutionary tool in the treatment of numerous hematological disorders. (revistadehematologia.org.mx)
  • Research explored its use in autoimmune disorders with excellent results. (revistadehematologia.org.mx)
Responses1
  • It also decreases IL-21- and TNF-producing T cell responses while promoting regulatory T cell responses without compromising the cytolytic activity of CD8 + T cells. (aai.org)
Lymphocyte2
  • The aim of the present study is to investigate the role of systemic inflammatory markers such as leukocytes (or white blood cells, WBCs), the neutrophil-to-lymphocyte ratio (NLR), and platelet distribution width (PDW), which are among the parameters of the complete blood count (CBC), in the prediction of rupture of EPs under MTX treatment. (bvsalud.org)
  • At 4 years of age and 29 months post-transplant, the patient demonstrated normal T-lymphocyte and natural killer cell numbers. (frontiersin.org)
Translational medicine1
Organs2
  • Rheumatoid arthritis (RA), an autoimmune disease, is characterized by inflammation that affects not only the liver but also other organs and the musculoskeletal system. (bvsalud.org)
  • Mechanistically, blocking OX40L-OX40 interaction with an anti-hOX40L reduces infiltration of human T cells in target organs, including liver, gut, lung, and skin. (aai.org)
Mesenchymal cell1
  • Engineered mesenchymal cell-based patches as controlled VEGF delivery systems to induce extrinsic angiogenesis. (unibas.ch)
Viral6
  • 1 T cells are engineered to express CARs through viral vectors, enabling them to recognize specific target antigens. (bdbiosciences.com)
  • T cells are modified ex vivo by transduction with viral vectors containing CAR or other modifications of interest. (bdbiosciences.com)
  • A promising approach is provided by the transduction of CLL cells with CD40 ligand (CD40L) by viral vectors to enhance their immunogenicity. (shengsci.com)
  • HSPCs are characterized by expression of the CD34 marker, which is used for cell enrichment followed by overnight transduction with a viral vector carrying the wild type FANCA gene, commonly mutated in FA. (fredhutch.org)
  • As long-lived viral DNA within specific cellular reservoirs is responsible for persistent hepatitis B virus, Herpes simplex virus, and human immunodeficiency virus infections, the discovery of AAV vectors with strong tropism for hepatocytes, sensory neurons and T cells, respectively, is of particular interest. (biomedcentral.com)
  • This review will summarize the most recent strategies to identify AAV vectors with enhanced tropism and transduction in cell types that harbor viral reservoirs. (biomedcentral.com)
Transduction1
  • Marker genes enable measurement of transduction and allow selection of transduced cells, whereas suicide genes allow selective deletion of administered T cells in the face of toxicity. (ashpublications.org)
Platelets1
  • p>Blood has three main cell types: red blood cells (which carry oxygen), white blood cells (which fight infection) and platelets (which clot the blood). (nemours.org)
Umbilical3
Pathogenic1
  • The pathogenic mechanisms involve a wide range of cells and soluble factors. (mdpi.com)
Gene2
  • Although challenges remain, investment in the field has started to bear fruit, with a number of gene and cell therapeutics reaching the market in the past decade. (bvsalud.org)
  • We have created a highly compact marker/suicide gene for T cells combining target epitopes from both CD34 and CD20 antigens (RQR8). (ashpublications.org)
Manifestations1
  • Autoimmune manifestations are also reported, predominantly hematological cytopenias, but arthritis and systemic lupus erythematosus are reported ( 3 ). (frontiersin.org)
GvHD1
  • Learn about the different GvHD prevention platforms, including pharmacological and cell manipulation strategies. (ebmt.org)
Lung5
  • Expression of the receptor for hyaluronic acid mediated motility (RHAMM) is associated with poor prognosis and metastasis in non-small cell lung carcinoma. (biomedfrontiers.org)
  • The receptor for hyaluronic acid-mediated motility (RHAMM) is upregulated in various cancers, but its role in primary and metastatic non-small cell lung carcinoma (NSCLC) remains to be determined. (biomedfrontiers.org)
  • Moreover, shRNA-mediated knockdown of RHAMM reduced the migratory ability of two lung adenocarcinoma cell lines, H1975 and H3255. (biomedfrontiers.org)
  • The two largest subtypes of lung cancer are adenocarcinoma and squamous cell carcinoma, which together account for about 80% of non-small cell carcinomas (NSCLS). (biomedfrontiers.org)
  • To further confirm that RHAMM is upregulated in NSCLC, we looked at mRNA levels in 57 paired lung adenocarcinomas and 49 pair lung squamous cell carcinomas from The Cancer Genome Atlas (TCGA). (biomedfrontiers.org)
Blood12
  • Autoimmune hemolytic anemia Hemolytic Anemia Hemolytic anemia (HA) is the term given to a large group of anemias that are caused by the premature destruction/hemolysis of circulating red blood cells (RBCs). (lecturio.com)
  • however, the cells were trackable in blood only. (issca.us)
  • In the last decade, the number of transplantations of HPCs derived from cord blood has increased, particularly for children. (nationalacademies.org)
  • Researchers at the NIH used RNA-Seq cells from skin and blood to study the underlying mechanisms in DIHS/DRESS and identified both HHV-6 and JAK-STAT pathways as potential targets. (hhv-6foundation.org)
  • New research line in automatic blood cell digital image classification. (cellsilab.com)
  • The NAC assembled an Irradiation Working Group to review current standards, published guidelines, and recent literature on the indications for irradiated components and the quality of irradiated red blood cell (RBC) components to facilitate recommendations for best practices. (nacblood.ca)
  • and recent publications on the quality of stored RBC post irradiation and practices involving irradiation of autologous blood collected by intraoperative cell salvage were consulted. (nacblood.ca)
  • 2015年11月11日, the TTS-JST Transplantation Science Mentee-Mentor Travel Award, International Basic Science Mentee-Mentor Awards-TSS2015, Bacterial pathogens provoke B cells responding to blood group A-antigens to be resistant to calcineurin inhibitors through TLR-MyD88 pathway. (hiroshima-u.ac.jp)
  • All blood cancers involve the degeneration of certain blood cells, which multiply uncontrollably and suppress the healthy blood-forming system. (dkms.org)
  • In support of this emerging potential for utilization of stem cells in regenerative medicine, Cord for Life ® now offers the option of storing cord blood in a 5-compartment cryo-bag. (cordforlife.com)
  • Storing the cord blood unit in five smaller volumes makes it possible to use a portion of the stem cells and save the rest for future use. (cordforlife.com)
  • Cord for Life ® 's 5-chamber cryo-bag offers maximum flexibility for storing cord blood stem cells - whether you need the entire unit for one of the 80+ FDA treatments or a portion of the unit for regenerative medicine. (cordforlife.com)
Infections1
  • While all cells are functionally deficient, resulting in high susceptibility to infections, only some of these cells are causing autoimmune symptoms. (hindawi.com)