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LamininAntibodiesAntibody SpecificityAntibodies, ViralReceptors, LamininAntibodies, MonoclonalAntibodies, BacterialAntibody FormationAntibodies, NeutralizingFluorescent Antibody TechniqueAntibody AffinityAutomobilesAntibodies, Anti-IdiotypicBinding Sites, AntibodyHIV AntibodiesEpitopesAntibodies, NeoplasmAntibodies, ProtozoanAntibodies, AntinuclearAutoantibodiesCross ReactionsMolecular Sequence DataImmunoglobulin MAntibodies, FungalAmino Acid SequenceNeutralization TestsBasement MembraneIsocyanatesAntigen-Antibody ReactionsAntibodies, BispecificMice, Inbred BALB CSingle-Chain AntibodiesAntibodies, BlockingImmunoglobulin GAntigen-Antibody ComplexImmunoglobulin Fab FragmentsAntibodies, HeterophileFluorescent Antibody Technique, IndirectAntibodies, CatalyticRabbitsImmune SeraCell LineCell AdhesionGasolineImmunoglobulin AAntibodies, Monoclonal, HumanizedHybridomasCells, CulturedEpitope MappingImmunohistochemistryImmunoenzyme TechniquesAntibodies, AntiphospholipidDermatitis, OccupationalFibronectinsImmunizationB-LymphocytesRecombinant ProteinsAntigensBlotting, WesternMolecular WeightAntigens, SurfacePaintSensitivity and SpecificityImmunoblottingElectrophoresis, Polyacrylamide GelAntigens, BacterialEnzyme-Linked Immunosorbent AssayFlow CytometryIntegrinsTime FactorsImmunization, PassiveAutoantigensImmunoassayImmunoglobulin FragmentsImmunologic TechniquesGlycoproteinsBase SequenceRadioimmunoassayPolyurethanesPeptide FragmentsAntigens, ViralTransplantation, AutologousExtracellular MatrixComplement Fixation TestsCattleCollagenProtein BindingHemagglutination TestsHemagglutination Inhibition TestsAntibodies, Antineutrophil CytoplasmicT-LymphocytesIntegrin alpha6beta1Antigens, NeoplasmImmunoglobulin Variable RegionSeroepidemiologic StudiesImmunosorbent TechniquesImmunoglobulin IdiotypesTumor Cells, CulturedMembrane GlycoproteinsBinding, Competitive
Anti-laminin-332CollagenMonoclonal antibodyAntigenCytokinesIntegrinProteinsTitersCross-reactiveInhibitMicePolyclonalMembranesNC16aGlomeruliAdhesionPemphigusHeparinAntigensGlomerularBullousConclusionsClinicalAutoimmuneBindPathwaysPatientsTissueBoundSerumPeptideInflammatoryKidneyInductionCellsSignificantlyOrganDiseaseHumanProductionDomainIndirectShownCellSkin
Anti-laminin-3323
  • The names 'acquired junctional epidermolysis bullosa', 'anti-laminin-332 mucous membrane pemphigoid (MMP)' and 'mixed auto-immune subepidermal blistering dermatosis' are proposed for dogs with clinical signs reminiscent of EBA, MMP or BP respectively. (morulaivf.com)
  • Mucous membrane pemphigoid (MMP) with anti-laminin 332 autoantibodies could also be related to malignancies, nevertheless, present serological assays have appreciable limitations. (aetox.com)
  • At current, no industrial take a look at for anti-laminin 332 antibodies is out there, limiting the prognosis to specialised laboratories worldwide. (korilog.com)
Collagen15
  • During the initial infection, alpha-helical M proteins (M8 and M13) on the surface of the streptococcus bind type IV collagen in the host, and this interaction can trigger auto-antibody formation. (medscape.com)
  • Immunological investigations consisted of direct immunofluorescence (IF), indirect IF with intact and salt-split canine gingival, and salt-split normal or laminin-332-deficient human skin, immunoblotting with purified human laminin-332 and immunoblotting with recombinant NC1 domain of human collagen VII. (morulaivf.com)
  • Several autoantibodies have been identified, including those against BPAG2, laminin-332, and type VII collagen. (msdmanuals.com)
  • Epidermolysis bullosa acquisita is caused by antibodies targeting type VII collagen, the major component of anchoring fibrils that connect the basement membrane to dermal structures. (medscape.com)
  • In canine epidermolysis bullosa acquisita, the immunoglobulin G (IgG) autoantibodies also target the type VII collagen noncollagenous (NC1) domain, which shares greater than 80% homology in amino acid sequence with the human NC1 domain. (medscape.com)
  • A subset of patients have variations in which either IgG autoantibodies bind the central triple-helical collagenous (NC2) domain of type VII collagen, or immunoglobulin A (IgA), rather than IgG, targets type VII collagen. (medscape.com)
  • [ 3 ] IgG autoantibodies specific for anchoring fibrils (type VII collagen) of the skin basement membrane have a heterogeneous subclass and light-chain composition, and their complement-activating capacities do not correlate with the inflammatory phenotype. (medscape.com)
  • Various murine models have contributed to the understanding of the pathogenic role of antitype VII collagen antibodies and pathophysiology of epidermolysis bullosa acquisita. (medscape.com)
  • Initial studies demonstrated that rabbit antimouse type VII collagen antibodies, as well as rabbit antibodies raised against human recombinant protein type VII collagen, were able to induce blisters in mice. (medscape.com)
  • [ 5 , 6 ] More recently, affinity-purified antitype VII collagen autoantibodies from epidermolysis bullosa acquisita patients have induced blisters in an adult hairless mouse strain (SKH1), further supporting a pathogenic role of antitype collagen VII autoantibodies. (medscape.com)
  • [ 7 ] The failure of rabbit antimouse type VII collagen antibodies to induce blisters in C5-deficient mice supports a role for complement activation in the disease pathogenesis. (medscape.com)
  • Induction of an active model of autoimmunity against type VII collagen in a hairless mouse strain (SKH1) revealed that regulatory T cells, which have been identified for their ability to inhibit the development of autoimmune diseases, do not inhibit the development of autoantibodies against the self-protein mouse-type VII collagen. (medscape.com)
  • Immunization of type VII collagen in athymic nude SJL mice did not induce an autoimmune response, whereas the repletion of T cells from type VII collagen-immunized wild-type mice to the thymic mice showed autoantibody production and resulted in a blistering disease phenotype, supporting the role of T cells in the induction of epidermolysis bullosa acquisita. (medscape.com)
  • Below the epidermis is the basement membrane which is a thin layer of delicate tissue containing collagen, laminins, and other proteins. (osmosis.org)
  • Depending on the type, the genetic mutation affects how different proteins in your skin, such as keratin, laminin, or collagen, are made. (born-wild.com)
Monoclonal antibody1
  • In vitro assays demonstrated a competition of laminin and this monoclonal antibody for the binding site on purified 5′-nucleotidase. (morulaivf.com)
Antigen7
  • however, rabbit antibodies raised against the recombinant proteins encoded by the gene of mouse bullous pemphigoid antigen 2 (BP180) are capable of inducing blisters in newborn mice in a complement-dependent manner. (medscape.com)
  • These observations establish laminin-332 as a novel basement membrane antigen in dogs with autoimmune blistering diseases with variable clinical phenotypes. (morulaivf.com)
  • Skin biopsy using transmission electron microscopy (TEM) and/or immunofluorescent antibody/antigen mapping can be performed but is no longer the preferred method of diagnosis. (nih.gov)
  • BALB/c mice immunized with multimeric DWEYSVWLSN develop IgG1 anti-DNA antibodies and glomerular immunoglobulin deposits, leading us to investigate the role of IL-4 in this model of antigen induced lupus. (elsevierpure.com)
  • This extensive immune cross-reactivity between SARS-CoV-2 antibodies and different antigen groups may play a role in the multi-system disease process of COVID-19, influence the severity of the disease, precipitate the onset of autoimmunity in susceptible subgroups, and potentially exacerbate autoimmunity in subjects that have pre-existing autoimmune diseases. (frontiersin.org)
  • Aim of the first part of this doctoral thesis was to investigate antigen-presenting cells (APC) and APC-derived cytokines, as well as their relation to T helper (Th) cell subsets and the auto-ab response in the pathogenesis of pemphigus. (uni-marburg.de)
  • Immune cells called B cells produce IgG antibodies , which are Y shaped molecules with 2 regions, an antigen binding fragment region - or Fab region , and fragment crystallizable region or Fc region . (osmosis.org)
Cytokines1
  • display and cytokines of auto-antigens to effector cells. (gasyblog.com)
Integrin15
  • BACKGROUND: Anti-α6ß4 integrin autoantibodies can be observed in some patients with mucous membrane pemphigoid. (bvsalud.org)
  • We have previously identified anti-α6ß4 integrin extracellular domain autoantibodies together with anti-BP180 NC16A antibodies in a patient with DPP-4 inhibitor-induced bullous pemphigoid. (bvsalud.org)
  • However, the significance and impact of anti-α6ß4 integrin antibodies are unknown. (bvsalud.org)
  • OBJECTIVES: To characterize anti-α6ß4 integrin extracellular domain autoantibodies in pemphigoid patients, to determine whether these antibodies inhibit laminin-α6ß4 integrin binding and to observe their systemic effects. (bvsalud.org)
  • METHODS: Anti-α6ß4 integrin autoantibodies were analyzed by staining cells expressing the extracellular region of α6ß4 integrin with sera from 20 patients with pemphigoid. (bvsalud.org)
  • The anti-α6ß4 integrin autoantibodies were characterized using different transfectants. (bvsalud.org)
  • The binding of laminins to α6ß4 integrin was studied using cells expressing the activated conformation of α6ß4 integrin and the inhibitory effect of the autoantibodies on the binding of laminins to α6ß4 integrin was tested. (bvsalud.org)
  • RESULTS: IgG autoantibodies against the extracellular domain of anti-α6ß4 integrin were found in some patients with pemphigoid. (bvsalud.org)
  • Laminin binding to α6ß4 integrin was observed in the active conformation of α6ß4 integrin, and serum from a patient with a high titer of anti-α6ß4 integrin antibodies inhibited the binding of both laminin-511 and laminin-332 to α6ß4 integrin. (bvsalud.org)
  • A reduction in the titer of the anti-α6ß4 integrin antibody was associated with improvement in both skin and gastrointestinal symptoms. (bvsalud.org)
  • CONCLUSIONS: This study demonstrated the presence of anti-α6ß4 integrin extracellular domain-specific autoantibodies in some patients with pemphigoid. (bvsalud.org)
  • In addition, these autoantibodies showed inhibitory activity on α6ß4 integrin-laminin binding. (bvsalud.org)
  • Antibodies to beta-4 integrin have been identified in generalized MMP and ocular MMP, and antibodies to alpha-6 integrin have been identified in oral MMP. (msdmanuals.com)
  • Synthetic peptides from the carboxy-terminal globular domain of the A chain of laminin: their ability to promote cell adhesion and neurite outgrowth, and interact with heparin and the beta 1 integrin subunit. (rupress.org)
  • Antibodies against the beta 1 integrin subunit inhibited HT-1080 cell adhesion and neurite outgrowth on surfaces adsorbed with peptides GD-3 and GD-4. (rupress.org)
Proteins3
  • We sought to determine whether immune reactivity occurs between anti-SARS-CoV-2 protein antibodies and human tissue antigens, and whether molecular mimicry between COVID-19 viral proteins and human tissues could be the cause. (frontiersin.org)
  • We found that SARS-CoV-2 antibodies had reactions with 28 out of 55 tissue antigens, representing a diversity of tissue groups that included barrier proteins, gastrointestinal, thyroid and neural tissues, and more. (frontiersin.org)
  • In this mechanism, antibodies formed against SARS-CoV-2 would also bind to human tissue proteins leading to autoimmune reactivity. (frontiersin.org)
Titers4
  • Trends in antibody titers and clinical symptoms were quantified and analyzed. (bvsalud.org)
  • The presence of high titers of autoantibodies against nuclear antigens is the hallmark of SLE. (biomedcentral.com)
  • Titers of IgG anti-laminin and anti-histone, but not anti-Sm/RNP and anti-cardiolipin antibodies, were significantly higher in the IL-4 wild type group. (elsevierpure.com)
  • Strikingly, in pemphigus patients, a significant correlation was not only found between serum IgG auto-ab titers and plasma levels of both IL-6 and TNF-α, but also between auto-ab titers and plasma concentrations of IL-27. (uni-marburg.de)
Cross-reactive2
  • bacterial or viral similarity with self-antigens (molecular mimicry) leading to production of cross-reactive antibodies. (pocketdentistry.com)
  • In either pre-nephritic NZB/W F1 or BALB/c mice the shot of anti-dsDNA antibodies would bring about immediate (cross-reactive) or indirect (chromatin-mediated) binding to mesangial cells [8 9 10 Prior studies also have confirmed that anti-dsDNA isolated from LN sufferers could bind to individual mesangial cells and its own binding activity correlated with disease activity [11]. (gasyblog.com)
Inhibit1
  • Polyclonal and monoclonal antibodies against chicken gizzard 5 '-nucleotidase inhibit the spreading process of chicken embryonic fibroblasts on laminin substratum. (morulaivf.com)
Mice3
  • Passive transfer experiments have demonstrated that purified autoantibodies from patients with the pemphigus group of diseases can induce blister formation when delivered to newborn mice. (medscape.com)
  • Following peptide immunization, IgG1 anti-peptide and anti-DNA antibodies were significantly higher in IL-4 wild type mice, while IgM and IgG3 anti-DNA levels were significantly higher in IL-4 knockout mice. (elsevierpure.com)
  • Affinity-purified autoantibodies to indigenous DNA isolated BMS-663068 from NZB/W F1 mice and two SLE sufferers with energetic LN exhibited cross-reactivity using the A and D SnRNP polypeptides and relationship with pig kidney cells [19]. (gasyblog.com)
Polyclonal3
  • Polyclonal and monoclonal antibodies raised against chicken gizzard 5′-nucleotidase were tested in adhesion assays of embryonic chicken fibroblasts (CEF) for their ability to interfere with the adhesion process of these cells on either laminin or fibronectin substrata. (morulaivf.com)
  • This effect was obtained with a polyclonal antibody as well as with one from 12 monoclonal antibodies raised against the native enzyme purified from chicken gizzard. (morulaivf.com)
  • We applied both human monoclonal anti-SARS-Cov-2 antibodies (spike protein, nucleoprotein) and rabbit polyclonal anti-SARS-Cov-2 antibodies (envelope protein, membrane protein) to 55 different tissue antigens. (frontiersin.org)
Membranes4
  • Mucous membrane pemphigoid (MMP) is characterized by subepithelial lesions caused by autoantibodies against molecules of epithelial basement membranes. (msdmanuals.com)
  • Merged pictures showed that laminin α5 and Lu-Fc co-localized at basement membranes. (bio-rad-antibodies.com)
  • Prednisone stabilizes lysosomal membranes and suppresses lymphocyte and antibody production. (medscape.com)
  • One of the components of the complex, alpha-dystroglycan binds both laminin of muscle (laminin-2) and agrin of muscle basement membranes. (rupress.org)
NC16a1
  • Immunoblot assays using human epidermal extracts and BP180 NC16a domain recombinant protein confirmed the presence of IgG antibodies against BP180 and recombinant BP180 NC16a domain protein. (morulaivf.com)
Glomeruli2
Adhesion4
Pemphigus4
  • Whereas the pemphigus subset of diseases is mediated by autoantibodies that target the extracellular skin components that link one epidermal cell to another, the pemphigoid subset is mediated by autoantibodies that target the extracellular skin components that link the skin basement membrane components either to the lowermost layer of epidermal cells or to the dermal components. (medscape.com)
  • Thus, the autoantibodies of patients with pemphigus foliaceus, which exclusively target desmoglein-1, are unable to break down the adherence of the upper layers of epithelium of oral mucosa, which is protected by the presence of desmoglein-3. (medscape.com)
  • The rare, but potentially fatal autoimmune disorder pemphigus is considered as a prototypical antibody-mediated organ-specific disease, in which immunoglobulin (Ig) G autoantibodies (auto-ab) mainly target the desmosomal cadherins Desmoglein 3 (Dsg3) and Dsg1 within the epidermis. (uni-marburg.de)
  • Although it has long been recognized that auto-ab crucially contribute to the pathology of pemphigus, the exact immunological events leading to the loss of self-tolerance have not yet been fully identified. (uni-marburg.de)
Heparin1
  • A range of anti-dsDNA binding goals on mesangial cells continues to be proposed plus they consist of annexin II α-actinin laminin or heparin sulphate [9 12 13 14 Within this framework the binding activity of anti-dsDNA to annexin II carefully links with disease activity in individual LN and glomerular annexin II appearance co-localizes with IgG and C3 debris and correlates with intensity of nephritis [9]. (gasyblog.com)
Antigens2
  • RESULTS: Serum from patients exhibited high levels of IgGs against nuclear antigens (gP210, Nup62, PCNA, Ro/SSA, Sm/RNP complex, SS-A/SS-B), components of the basement membrane (entactin, laminin), fibrinogen IV and gliadin. (herseninstituut.nl)
  • Systemic lupus erythematosus (SLE) is a complex disease characterized by the appearance of autoantibodies against nuclear antigens and the involvement of multiple organ systems, including the kidneys. (biomedcentral.com)
Glomerular1
  • Nucleosomes may also be important intra-renal goals of autoantibodies and the increased loss of intra-renal nuclease would promote nucleosome deposition and therefore the advancement and binding of autoantibodies [15 16 The current presence of circulating chromatin fragments is certainly very important to glomerular mesangial matrix deposition of anti-dsDNA antibody-containing immune system complexes in murine LN [10]. (gasyblog.com)
Bullous2
  • Laminin-332 polypeptides are targeted by auto-antibodies in human patients with mucous membrane (cicatricial) pemphigoid or, more rarely, subepidermal vesicular diseases that resemble epidermolysis bullosa acquisita (EBA) or bullous pemphigoid (BP). (morulaivf.com)
  • Bullous pemphigoid is a type II hypersensitivity reaction , which is when the immune system produces antibodies that bind to the body 's own cells. (osmosis.org)
Conclusions1
Clinical2
  • Despite the wide diversity in clinical symptoms and biological processes, they all share the presence of autoantibodies and/or autoreactive T cells. (pocketdentistry.com)
  • These novel finding highlights a potential clinical application of laminin 411 induced-IPCs in the treatment of T1DM, which calls for further studies. (biomedcentral.com)
Autoimmune4
  • As a group, autoimmune blistering skin diseases are recognized as autoantibody-mediated diseases. (medscape.com)
  • Highly susceptible mouse strains such as SJL/N, A.SW, B10.S (H-2 s ) develop multiple autoimmune manifestations after exposure to inorganic mercury, including lymphoproliferation, elevated levels of autoantibodies, overproduction of IgG and IgE, and circulating immune complexes in kidney and vasculature. (biomedcentral.com)
  • For the majority of autoimmune diseases, autoantigens have been identified as the targets of autoantibodies and autoreactive T cells 3 . (pocketdentistry.com)
  • A cohort of twelve patients suffering from the autoimmune muscle weakness myasthenia gravis (MG) served as a control for a further unrelated auto-ab-mediated organ-specific disorder. (uni-marburg.de)
Bind1
  • Autoantibodies in one of these sufferers bind mostly towards the cell surface area and led to very much significant complement-mediated cytolysis in comparison with the individual whose autoantibodies had been internalized [19]. (gasyblog.com)
Pathways2
  • Furthermore, the expression of the genes known to govern insulin expression including Pdx1 and Ngn3 was markedly induced by laminin 411, which suggests that Pdx 1 and Ngn 3 signaling pathways are involved in laminin 411 induced-insulin expression machinery. (biomedcentral.com)
  • Our results demonstrate that laminin 411 acts as a potent differentiation inducer of IPCs from UC-MSCs via the Pdx1 and Ngn3 signaling pathways. (biomedcentral.com)
Patients4
  • METHODS: Using a multiplex microarray, we assessed the spectrum of serum autoantibodies in 56 genetically confirmed patients with AGS. (herseninstituut.nl)
  • Upon testing whether antibodies in AGS could be found in the central nervous system, IgGs were identified to target in vivo endothelial cells in vivo and astrocytes in brain sections of deceased patients with AGS. (herseninstituut.nl)
  • Some of these autoantibodies target endothelial cells and astrocytes in the brain of the affected patients, perhaps explaining the prominence of neurological disease in the AGS phenotype. (herseninstituut.nl)
  • Very recently, human monoclonal antibodies were approved for use on patients with COVID-19. (frontiersin.org)
Tissue3
Bound2
Serum2
  • Serum autoantibodies tend to be absent or at low titre. (msdmanuals.com)
  • According to our present understanding, the immunological defects resulting in the development of SLE can be categorized into two phases: (a) systemic autoimmunity resulting in increased serum antinuclear and antiglomerular autoantibodies and (b) immunological events that occur within the target organ and result in end organ damage. (biomedcentral.com)
Peptide1
  • We conclude that while IL-4 does not materially affect the generation of some autoantibody responses associated with peptide induced autoimmunity, IL-4 deficiency inhibits kidney immunoglobulin deposition. (elsevierpure.com)
Inflammatory1
  • Exposure to gluten is the starting point of an inflammatory cascade capable of forming autoantibodies that are brought to the skin, where they are deposited, culminating in the formation of skin lesions. (scielo.br)
Kidney3
Induction2
  • There was no evidence for mercury induction of this autoantibody. (biomedcentral.com)
  • UC-MSCs were then cultured in the IPC induction and differentiation medium in the presence of laminin 411. (biomedcentral.com)
Cells7
  • The initial attachment process of CEF on fibronectin and laminin substrata was not influenced by preincubating these cells with antibodies against chicken gizzard 5′-nucleotidase. (morulaivf.com)
  • However, the subsequent spreading process of these cells was found to be inhibited for at least 2 h on a laminin substratum. (morulaivf.com)
  • Spreading-arrested and rounded CEF do not develop prominent intracellular stress-fibers like control cells, instead they seem to concentrate their available actin in areas of presumptive initial contact with the laminin substratum. (morulaivf.com)
  • Although these studies warrant independent confirmation, they support the notion that apoptotic cells could harbor the immunogens responsible for antinuclear antibody formation. (biomedcentral.com)
  • Laminin 411 markedly induced the expression of the genes Foxa2 and Sox17 , markers for pancreatic precursor cells, efficiently induced IPC differentiation from MSCs, and up-regulated insulin expression at both mRNA and protein levels. (biomedcentral.com)
  • We have reported that mast cells adhere to laminin after activation with PMA. (aai.org)
  • however, culture of mast cells with TGF-beta 1 was determined to enhance IgE-mediated adherence to laminin. (aai.org)
Significantly1
  • More importantly, administration of laminin 411-induced IPCs rapidly and significantly down-regulated fasting blood glucose levels, significantly reduced the HbA1c concentration and markedly improved the symptoms and survival of T1DM rats. (biomedcentral.com)
Organ2
  • However, it remains uncertain why the brain is the most affected organ, and little is known about the presence of autoantibodies in AGS. (herseninstituut.nl)
  • The hyperactivated leukocytes as well as the pathogenic autoantibodies and immune complexes resulting from the systemic disease ultimately play an important role in local autoimmunity and end organ disease within target organs like the kidneys. (biomedcentral.com)
Disease1
  • Elevated anti-α-actinin antibodies titres are discovered ahead of or at disease starting point in LN sufferers in comparison to energetic or inactive lupus sufferers who didn't have proof nephritis [12]. (gasyblog.com)
Human4
Production1
  • They may decrease autoantibody production and increase solubilization and removal of immune complexes. (medscape.com)
Domain1
  • This study was conducted to define the potential sequence(s) originating from the G domain of laminin with any of these functional activities. (rupress.org)
Indirect1
Shown1
  • A similar increase of expression on day two was previously shown for laminin alpha 1 chain. (rupress.org)
Cell1
  • Hence, the high-affinity IgE-R on the mast cell functions not only in exocytosis but also facilitates the process of mast cell adherence to laminin. (aai.org)
Skin1