• The European Collection of Authenticated Cell Cultures houses and supplies cell lines. (wikipedia.org)
  • High expression of RAD18 facilitated a highly stem-cell phenotype through the Hippo/YAP pathway, which supports the proliferation of TNBC. (nature.com)
  • Lipotransfection and MTT were applied for proliferation assessment in U87-MG cells. (hindawi.com)
  • To infer functional relationships between SLCs and metabolites, we developed a strategy to identify SLCs able to sustain cell viability and proliferation under growth-limiting concentrations of essential nutrients. (life-science-alliance.org)
  • One-by-one depletion of 13 amino acids required for cell proliferation enabled gain-of-function genetic screens using a SLC-focused CRISPR/Cas9-based transcriptional activation approach to uncover transporters relieving cells from growth-limiting metabolic bottlenecks. (life-science-alliance.org)
  • Intestinal-specific deletion of Pcdh20 damages epithelial cell proliferation and differentiation and causes morphological changes, including shortened crypts and microvilli. (biomedcentral.com)
  • The degree of methylation affects the expression of target genes and has a significant impact on cell proliferation, apoptosis, cytokine secretion, and cell activity ( 6 , 7 ). (frontiersin.org)
  • Leonotis nepetifolia mediated silver nanoparticles effectively reduced the proliferation of pancreatic and ovarian cancer cell lines than that of synthetic nanoparticles. (ijpsonline.com)
  • ECACC is one of the first collections of authenticated cell cultures worldwide and now holds cell lines from 45 species including 50 tissue types, 300 HLA types, over 800 genetic disorders and roughly 450 monoclonal antibodies. (wikipedia.org)
  • If you're struggling with setting up and maintaining your cell cultures, you're not alone. (coleparmer.com)
  • Growing viruses in cell cultures allowed preparation of purified viruses for the manufacture of vaccines . (wikipedia.org)
  • This vaccine was made possible by the cell culture research of John Franklin Enders , Thomas Huckle Weller , and Frederick Chapman Robbins , who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures. (wikipedia.org)
  • There are two basic systems for growing cells: adherent and suspension cultures. (integra-biosciences.com)
  • Adherent cultures are grown on an artificial substrate, whereas cells grown in suspension are free-floating in the culture medium. (integra-biosciences.com)
  • While only a few cell types naturally grow in suspension (e.g. lymphocytes), many adherent cell types can be adapted to suspension cultures. (integra-biosciences.com)
  • The first advantage of suspension cultures is that it's easier to passage the cells, as you don't need to detach them from a culture vessel by enzymatic or mechanical dissociation. (integra-biosciences.com)
  • Secondly, suspension cultures are easier to scale up, as the cell growth is only limited by their concentration in the medium, not by the available surface area. (integra-biosciences.com)
  • The major downside of suspension cultures is that they require daily cell counts and viability determination to follow growth patterns, whereas adherent cultures can easily be inspected under a microscope. (integra-biosciences.com)
  • Culturing suspension cells can be tricky because they need specialized culture vessels and techniques to maintain their viability and growth. (coleparmer.com)
  • Reexpression of RUNX3 in U87-MG cells significantly reduced glioma cell viability compared to control transfection. (hindawi.com)
  • We'll provide you with tips on reproducibility, contamination, viability and automation to help you overcome the major cell culture challenges. (integra-biosciences.com)
  • Treatment of pancreatic and ovarian with different concentrations of silver nanoparticles inhibited the cell viability with half maximal inhibitory concentration values of 25.4 μg/ml and 23.9 μg/ml. (ijpsonline.com)
  • ECACC, which was established in 1985, consists of a team with specialist knowledge which supply authenticated cell lines, induced Pluripotent stem cells (iPSCs) and nucleic acids to provide stock for the research community. (wikipedia.org)
  • All ECACC products can be found at the culture collections website alongside a range of services such as Mycoplasma testing and training courses for cell culture. (wikipedia.org)
  • ATCC Cell Line Land gives you access to standardized, authenticated and reproducible cell line 'omics data characterizations which can be traced back to physical lots of cells in ATCC's biorepository. (qiagen.com)
  • Despite the different approaches and techniques, all experiments have one thing in common: it's difficult to grow viable cells in the desired quantity to obtain reproducible results. (integra-biosciences.com)
  • The survival prognosis of human papillomavirus (HPV)-positive and HPV-negative head and neck squamous cell carcinoma (HNSCC) is largely different, and little is known about the anti-tumor mechanism of tumor-infiltrated exhausted CD8 + T cells (Tex) in HNSCC. (elifesciences.org)
  • One cell line named Penl1 was isolated from a lymph node metastasis (LNM) of penile squamous cell carcinoma (PeSCC), usual type and comprehensively characterized here. (oncotarget.com)
  • We've collected this data into ATCC Cell Line Land, our continually growing database of cell line 'omics data from both common and novel human and mouse cell lines, primary tissues and cells from ATCC. (qiagen.com)
  • Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
  • [12] He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
  • Mass spectrometry-based proteomics now enables genome-scale measurements of proteomes from diverse cells, tissues, and organisms. (biorxiv.org)
  • Expression of DCT was absent or barely detected in retina tissues, various human ocular cells, and major organs. (molvis.org)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • We successfully established two PeCa cell lines from fresh tumor tissues from 21 cases. (oncotarget.com)
  • In the past, techniques like isoenzyme analysis and karyotyping were used for cell line identification. (the-dna-universe.com)
  • The analysis of isoenzyme polymorphism is the standard method in quality control of cell line identification and interspecies contamination and is routinely use by main Biological Resource Centers. (fapesp.br)
  • Adherent cells such as fibroblasts, epithelial cells, and endothelial cells need a proper surface to attach to, and improper attachment can lead to cell death. (coleparmer.com)
  • Metastatic spread is responsible for the majority of cancer-associated deaths and is affected by a variety of factors, including the deregulation of gene expression, signal transduction, and epigenetic modification, as well as epithelial-to-mesenchymal transition (EMT) and the transformation of tumor cells into stem/progenitor cells. (jcancer.org)
  • Several mammalian cell lines, including CHO, lymphoma (NS0, SP2/0) and human embryonic kidney (HEK) cells, have been developed and used to produce therapeutic proteins and antibodies at high levels. (sigmaaldrich.com)
  • From the early 20th century, animal cell lines have been used for studies in medicine, cell biology, genetics, cancer research and drug development. (the-dna-universe.com)
  • Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
  • The aim of the study was to elucidate RUNX3 changes in different regulation levels of molecular biology starting from epigenetics to function in particular cases of astrocytic origin tumours of different grade evaluating significance of molecular changes of RUNX3 for patient clinical characteristics as well as evaluate RUNX3 reexpression effect to GBM cells. (hindawi.com)
  • It is an in vitro tool that aids the understanding of cell biology and the mechanisms of diseases. (integra-biosciences.com)
  • That's why we've teamed up with ATCC, the market leader in global biological materials and authenticated cell lines, to deliver you manually curated cell line 'omics data from the most popular cell lines offered by ATCC. (qiagen.com)
  • Additionally, RAD18 functions beyond DNA repair and may regulate many other key biological processes, such as chromatin strengthening, cell survival/death, stemness, and differentiation. (nature.com)
  • By performing cell biological and biochemical experiments using human cells, Moyer and Holland show that PLK4 interacts with a protein called STIL that is found in the central part of the centriole. (elifesciences.org)
  • 4 In cell culture assays, a large proportion of the reproducibility issues come from biological variation between passages or generations of cells. (integra-biosciences.com)
  • The American Type Culture Collection (ATCC) and American National Standards Institute (ANSI) have documented (ASN-0002) the whys and hows of human cell line identification. (the-dna-universe.com)
  • The ANSI/ATCC standard ASN-0002 has mentioned STR profiling as the preferred method for human cell line authentication. (the-dna-universe.com)
  • ATCC was selected by The Michael J. Fox Foundation for Parkinson's Research (MJFF) to provide vital cell lines to academic, pharmaceutical, and biotechnology organizations committed to speeding the progress toward a cure for Parkinson's disease. (atcc.org)
  • In this first of three interviews, Dr. Brian Shapiro, a Marketing Segment Manager at ATCC, met with Dr. Elisia Clark, an Associate Director of Research Programs at MJFF, and Dr. Birgitt Schüle, an Associate Professor in the Department of Pathology at Stanford University School of Medicine, to discuss MJFF's mission and how the cell lines made available through the collaboration between MJFF and ATCC aids Parkinson's Disease research. (atcc.org)
  • Dr. Shapiro: Good afternoon, Elisia and Birgitt, thank you for meeting with me today to discuss The Michael J. Fox Foundation for Parkinson's Research (MJFF) and the cell models that were made available through a collaboration between MJFF and ATCC. (atcc.org)
  • MJFF has two methods for making preclinical tools available, one is the Tool Generation Program in which MJFF generates novel tools, such as the LRRK2 mutant MEF lines available through ATCC. (atcc.org)
  • The other is the MJFF Sponsored Tools Program, which identifies preclinical tools developed by external research investigators, such as the patient-derived iPSC lines generated by Dr. Birgitt Schüle, and connects them with repositories, such as ATCC, for widespread distribution. (atcc.org)
  • Dr. Shapiro: There have been several Synuclein Alpha (SNCA) knockout isogenic cell lines that were made available through the collaboration between MJFF and ATCC. (atcc.org)
  • Advances in gene editing such as CRISPR-Cas systems further enhance production efficiency and improve product quality through modification of host cell genomes to manipulate cell growth and apoptosis, and to promote post-translational modification of the gene product. (sigmaaldrich.com)
  • Our data indicate that plakoglobin promotes tumor cluster formation in IMPC and downregulates apoptosis in the cell clusters through activation of PI3K/Akt/Bcl-2 signaling. (jcancer.org)
  • In 1907 the zoologist Ross Granville Harrison demonstrated the growth of frog embryonic cells that would give rise to nerve cells in a medium of clotted lymph . (wikipedia.org)
  • Adherent cells can quickly reach confluency, meaning the entire surface of the culture vessel is covered with cells, which can lead to nutrient depletion and cell death. (coleparmer.com)
  • Lastly, detaching adherent cells from the surface for passaging or analysis can be tough and can lead to cell damage. (coleparmer.com)
  • For adherent cells, use tissue culture flasks or tissue culture dishes with the right coating to promote cell attachment and growth. (coleparmer.com)
  • Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture . (wikipedia.org)
  • There are two reasons for culturing naturally adherent cells in suspension. (integra-biosciences.com)
  • In 2D applications, adherent cells are grown in a monolayer system on a flat surface, e.g. in a T-flask. (integra-biosciences.com)
  • Scaffold-based 3D methods usually involve growing adherent cells in hydrogel scaffolds. (integra-biosciences.com)
  • Despite the varied causes of irreproducibility from factors that are difficult to control, cell line authentication (CLA) is a relatively simple operation that assures that the cells researchers believe they are working with are actually those cells, and that they are contaminant-free. (labmanager.com)
  • Cell line authentication - Needed for publishing, but how to do it? (the-dna-universe.com)
  • Many leading peer reviewed journals such as Nature, AACR Journals, Cell Biochemistry and Biophysics, etc. now require proof of cell line authentication before the submission of cell culture-based research articles. (the-dna-universe.com)
  • STR profiling, however, is a reliable, low cost and easy method of cell line authentication. (the-dna-universe.com)
  • Our laboratory intends to perform and to available this valuable tool of the authentication of the cell lines. (fapesp.br)
  • Evidence suggests that up to a third of established tumor cell lines being used for research are affected by inter- or intra-species cross-contamination or have been wrongly identified. (labmanager.com)
  • It was during the year 1968 when researchers first identified cross-contamination of cell lines. (the-dna-universe.com)
  • It has been estimated that a large proportion of the utilised cell lines are affected by the persistent problem of contamination or are even misidentified. (the-dna-universe.com)
  • Say goodbye to cell culture contamination nightmares and hello to happy cells. (coleparmer.com)
  • The authenticity and certification of cell lines used in biomedical research is very important to ensure reliable results and to avoid misidentification and cross-contamination among the cells. (fapesp.br)
  • On the other hand, the short tandem repeat profiling have been developed to authenticate human cell lines detecting intraspecies contamination too. (fapesp.br)
  • Interestingly, P-Tex cells expressed CDK4 genes as high as cancer cells, which could be simultaneously inhibited by CDK4 inhibitors and might be a potential reason for the ineffectiveness of CDK4 inhibitors in treating HPV-positive HNSCC. (elifesciences.org)
  • The E3 ubiquitin ligase RAD18 is well known for the maintenance of genome stability and cell survival through many DNA damage response (DDR) pathways such as translesion DNA synthesis (TLS) and homologous recombination repair (HRR) [ 4 , 5 ]. (nature.com)
  • P-Tex cells could aggregate in the antigen-presenting cell niches and activate certain signaling pathways. (elifesciences.org)
  • BEX proteins have been reported to be involved in transcriptional regulation and signaling pathways in neurodegeneration, cell cycle and tumor growth [ 5 - 8 ]. (ijbs.com)
  • Fig 1: Difference between human primary cell culture and cell line (Oyeleye, O. et al . (the-dna-universe.com)
  • What are the challenges of culturing primary cells compared to established cell lines? (coleparmer.com)
  • Culturing primary cells presents unique challenges compared to established cell lines. (coleparmer.com)
  • Primary cells have a limited lifespan and demand careful handling and specialized conditions to maintain their original properties. (coleparmer.com)
  • Data mining for 5′-end CpG island methylation of TUSC3, ATRNL1, POMT1 and SAMD4A in cancer cell lines and primary tumors showed that the epigenetic defect was commonly observed among different tumor types in association with the diminished expression of the corresponding transcript. (oncotarget.com)
  • Bioreactors provide controlled microenvironments and nutrient delivery to regulate cell growth and differentiation, improving standardization and reproducibility. (sigmaaldrich.com)
  • To maintain accuracy in research results, verifying the authenticity of human cell lines is cr. (shmooze.com)
  • Presumably, the stem cells were changed in their nascent stage with those of Mizmedi because a total of six related researchers do not have any doubts as to the authenticity of stem cells. (blogspot.com)
  • BEX2 can also promote cell migration and invasion in glioma [ 7 ]. (ijbs.com)
  • Molecular methods targeted to DNA mitochondrial (mtDNA) has been used mainly for identifying or confirming the species of origin of animal cell lines. (fapesp.br)
  • The aim of this work is the deployment in our laboratory of one molecular technique (mtDNA) for species identification of animal cell lines besides an improvement of the electrophoretic analysis of isoenzymes which will be used as control and pattern. (fapesp.br)
  • To establish penile cancer (PeCa) cell lines for the study of molecular mechanisms of carcinogenesis and testing therapeutic reagents. (oncotarget.com)
  • However, the mechanism through which RAD18 influences triple-negative breast cancer (TNBC), especially the interaction between tumor cells and the tumor microenvironment, remains elusive. (nature.com)
  • This study provides fundamental insight into the functional impact of CDK4 inhibition on cells in the tumor microenvironment, which is of high importance and interest to the field. (elifesciences.org)
  • Macrophages are the most abundant white blood cells in the immune microenvironment and have a high degree of heterogeneity. (frontiersin.org)
  • models because of inhibiting the A2aR on T-cells where it features as an immune system checkpoint, adding to immune system evasion in the tumor microenvironment. (nanoker-society.org)
  • Have you ever tried to access the genomic profiles of cell lines before using them in your preclinical experiments just to come up empty-handed? (qiagen.com)
  • Maybe you've scoured public databases for gene expression information on your cell line to find the reported genomic profile doesn't match the cell line data you recently purchased? (qiagen.com)
  • We understand that knowing the genomic identity of your cell lines is fundamental to the success of your preclinical experiments. (qiagen.com)
  • We benchmarked PG2 using synthetic data and genomic, transcriptomic, and proteomic analysis of human leukemia cells. (biorxiv.org)
  • The cell lines were derived from a patient with an SNCA genomic triplication, who was a descendent of the "Iowa kindred," a multi-generational family hailing from Iowa. (atcc.org)
  • We reported here that plakoglobin, as a key component in cell adhesion, can promote collective metastasis through facilitating IMPC clusters formation. (jcancer.org)
  • In summary, our findings suggest that BEX2 negatively modulates the hedgehog signaling pathway by retaining Zic2 in the cytoplasm in colorectal cancer cells, thereby inhibiting migration and metastasis of colorectal cancer cells. (ijbs.com)
  • Dendritic cells (DCs), which are the most potent antigen-presenting cells in the human body, play a pivotal role in the immune response. (jcancer.org)
  • Misidentified and contaminated cell lines contribute significantly to irreproducible science. (labmanager.com)
  • Cell culture techniques were advanced significantly in the 1940s and 1950s to support research in virology . (wikipedia.org)
  • After the co-culture, the increase in pro-inflammatory cytokine expression in the mutant cells was significantly lower than that in the control group, while that in immunosuppressive factors was not significantly different. (frontiersin.org)
  • Resistin significantly promoted the expression of inflammatory proteins in AML cells. (frontiersin.org)
  • The researchers noted that overall characteristics and behaviour of their cell lines changed over time Eventually, they determined that contaminating agents such as adventitious microbes or other cell lines caused the changes. (the-dna-universe.com)
  • When a lab receives a cell line, relevant data regarding its origin, growth characteristics and culture conditions should be obtained (Reid et al . (the-dna-universe.com)
  • We performed cell-level multi-omics sequencing on human HNSCC samples to decipher the multi-dimensional characteristics of Tex cells. (elifesciences.org)
  • In addition, most of the stem cell lines were turned to stem cells of which the existence and DNA characteristics were not disclosed. (blogspot.com)
  • Comparative histomorphology, genetic characteristics, and protein expression patterns revealed essential similarities between the cell line and its corresponding LNM. (oncotarget.com)
  • Herein, we demonstrated that BEX2 knockout resulted in enhanced migratory and metastatic potential in colorectal cancer cells both in vitro and in vivo, and re-expression of BEX2 in knockout cells could reverse the enhanced migratory capacity. (ijbs.com)
  • Cultured cells are used for the manufacturing of biologics such as vaccines, therapeutic proteins and antibodies, and cell therapy products. (sigmaaldrich.com)
  • Although bacterial, yeast, and insect cell expressions systems are capable of overexpressing recombinant proteins, mammalian cells are most commonly used for biologics production due to their ability to propagate human viruses, express monoclonal antibodies, and incorporate post-translational modifications such as glycosylation that are critical to the production of effective biologics. (sigmaaldrich.com)
  • Our HCC signature covered well-established liver cancer hallmarks, and network analyses revealed coordinated interaction between several MRs. One novel MR, SEC14L2 , exerted an anti-proliferative effect in HCC cells and strongly suppressed tumor growth in a mouse model. (aging-us.com)
  • STR profiling establishes a very unique fingerprint of every human cell line (or any other species). (the-dna-universe.com)
  • This suggests that the modification of STIL by PLK4 is important for normal cell division across different species. (elifesciences.org)
  • AgNPs were toxic to the cells, which can lead to damage of deoxyribonucleic acid, generation of reactive oxygen species, mitochondrial damage and apoptotic induction[ 7 , 8 ]. (ijpsonline.com)
  • Cell lines that were obtained from major cell culture collections or fellow researchers have since been used for the development of vaccines, drugs, reconstructive medicine and HIV treatment and testing. (the-dna-universe.com)
  • Researchers keep on using misidentified cell lines even after they are unmasked. (the-dna-universe.com)
  • After sending data to one of former Mizmedi researchers, we learned the stem cells were actually made in Mizmedi. (blogspot.com)
  • And for feeder cells, use flasks or dishes with a proper surface and growth media to support their growth and function. (coleparmer.com)
  • Bioreactor flasks are specialized vessels used to culture cells at a large scale, making them a valuable tool for the production of mAbs. (coleparmer.com)
  • While hybridoma cells can be cultured in standard culture vessels, bioreactor flasks provide several advantages when scaling up the production process. (coleparmer.com)
  • Bioreactor flasks have a large surface area and volume, allowing for increased cell density and greater antibody production. (coleparmer.com)
  • The typical seed train cell expansion process involves thawing cryopreserved cells followed by successive expansions into larger culture vessels including flasks, spinners, and stirred bioreactors. (sigmaaldrich.com)
  • Bovine leukemia virus (BLV), a deltaretrovirus, causes B-cell leukemia/lymphoma in cattle and is prevalent in herds globally. (cdc.gov)
  • Bovine leukosis (B-cell leukemia/lymphoma), first described in 1871 in Lithuania, was believed to be an infectious disease because it spread through herds of cattle. (cdc.gov)
  • The lifespan of most cells is genetically determined, but some cell-culturing cells have been "transformed" into immortal cells which will reproduce indefinitely if the optimal conditions are provided. (wikipedia.org)
  • To tackle this issue, we have used cancer cells genetically deficient for the DNA methyltransferase enzymes in conjuction with circRNA expression microarrays. (oncotarget.com)
  • Overall, this gain-of-function approach using human cells uncovered functional transporter-nutrient relationships and revealed that transport activity up-regulation may be sufficient to overcome environmental metabolic restrictions. (life-science-alliance.org)
  • HEK-293 cells had been seeded (10,000 cells/well) in 96-well tradition plates and incubated at 37C within an atmosphere with 5% CO2 in Eagle moderate nutrient blend F-12, including 10% fetal leg serum and 1% l-glutamine. (nanoker-society.org)
  • TAMs are the major inflammatory cells in the TME, and are closely related to poor outcomes in several types of tumors [ 11 ]. (nature.com)
  • Using mouse models, we validated the metastatic potential of tumor clusters was higher than single cells in vivo . (jcancer.org)
  • Due to their simplicity, 2D techniques can't mimic the cells' in vivo environment, where they usually grow in three dimensional structures with complex cell-to-cell interactions. (integra-biosciences.com)
  • Both in vitro and in vivo data showed overexpression of plakoglobin in both the cell membrane and the cytoplasm of IMPC clusters. (jcancer.org)
  • The DNMT3A-mutated AML cells attenuated M1 macrophage polarization and resisted its killing effect in vitro and in vivo . (frontiersin.org)
  • The American embryologist Ross Granville Harrison developed the first in vitro cell culture technique at the very beginning of the twentieth century, when he successfully grew tissue fragments from frog embryos outside the body. (integra-biosciences.com)
  • In response to endogenous and exogenous insults, malignant cells mostly have an intensive DNA repair capacity that allows them to proliferate and survive. (nature.com)
  • or (f) living vectored cells expressing specific heterologous antigens. (who.int)
  • Modified amino acids are inhouse manufactured amino acid derivatives with specific properties enabling the intensification of cell culture processes. (sigmaaldrich.com)
  • The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
  • The results presented by Moyer and Holland help us to understand how dividing cells build the complex machinery that enables them to pass on their genetic material accurately. (elifesciences.org)
  • The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture . (wikipedia.org)
  • Traditional methods have relied on the production of viral agents in cells cultured in medium supplemented with serum, most commonly fetal bovine serum (FBS). (sigmaaldrich.com)
  • In 1951, the widely known HeLa cell line was isolated from the cervical cancer biopsy of Henrietta Lacks. (the-dna-universe.com)
  • Already in 1981, a report suggested that a large number of cell lines are contaminated with HeLa (more than 190 entries) followed by T-24 (20 entries) and HT-29 (18 entries) (Horbach and Halffman, 2017). (the-dna-universe.com)
  • Hela-A2A and Hela-A3 cells had been obtained internal. (nanoker-society.org)
  • hA1), HeLa (hA2A and hA3), and HEK-293 (hA2B) cells had been utilized. (nanoker-society.org)
  • Centrosomes nucleate the formation of the microtubule cytoskeleton in interphase cells and form the poles of the mitotic spindle during cell division. (elifesciences.org)
  • According to classification system of the World Health Organisation (WHO) based on histological evaluation, brain tumour grade is determined by necrotic cells in the centre of the tumour, increased mitotic activity, the presence of nuclear pleomorphism, and angiogenesis. (hindawi.com)
  • Chen J, Wang S, Jia S, Ding G, Jiang G, Cao L. Integrated Analysis of Long Non-Coding RNA and mRNA Expression Profile in Pancreatic Cancer Derived Exosomes Treated Dendritic Cells by Microarray Analysis. (jcancer.org)
  • A cytotoxicity study was conducted on two human cancer cell lines, pancreatic and ovarian, using sulforhodamine B assay. (ijpsonline.com)
  • When culture volume and cell density are optimal, the culture is then transferred to a production bioreactor. (sigmaaldrich.com)
  • Agitation based: a cell suspension is placed in a rotating bioreactor. (integra-biosciences.com)
  • These cells are free-flowing which means they don't require a surface to grow on. (coleparmer.com)
  • The right culture vessel is crucial here, and different cell types may require different surfaces, such as plastic, glass, or coated surfaces. (coleparmer.com)
  • Because cell lines are unique and require different nutrients to thrive, culture biomass and protein production are largely dependent on the culture media used. (sigmaaldrich.com)
  • All meetings require at least one Teams authenticated user to "start" the meeting. (uillinois.edu)
  • Once authenticated, you can further require that the user account be checked to ensure the user is authorized for network access. (cisco.com)
  • Forced-floating: a cell suspension is loaded into the wells of a low adhesion polymer-coated microplate. (integra-biosciences.com)
  • The apical junctional complex comprises tight junctions, adhesion junctions, and desmosomes, which seal adjacent cells, regulate paracellular transportation, and maintain intestinal barrier function, acting as a dynamic mechanical barrier [ 5 ]. (biomedcentral.com)
  • In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes , especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture , fungal culture, and microbiological culture (of microbes ). (wikipedia.org)
  • Seoul National University will probe doubts raised about (Hwang's) 2005 thesis first and, if the doubts are confirmed, will replicate experiments," a university statement said, referring to a study, published in May, on tailor-made human stem cells. (blogspot.com)
  • For example, RAD18 plays a vital role in regulating the DNA stability of embryonic stem cells and cellular homeostasis in highly prolific cells [ 6 ]. (nature.com)
  • At Schatten's request, the American school is investigating Hwang's work, with its probe focused on the pictures of the stem cells placed in Science. (blogspot.com)
  • So we provided five stem cells and somatic cells of patients to MBC. (blogspot.com)
  • Since we heard of the allegations that our stem cell lines came from Mizmedi, we turned to address that possibility. (blogspot.com)
  • Our team is thawing five frozen stem cell lines to authenticate our performance. (blogspot.com)
  • We staged an in-house probe late last month on how our tailor-made stem cells were replaced with those of Mizmedi. (blogspot.com)
  • The fact adds suspicions that genders of somatic cell contributors correspond with those of Mizmedi stem cells. (blogspot.com)
  • But we still need to confirm the veracity of five stem cells and that the 2004 stem cells really exist. (blogspot.com)
  • Multiple Myeloma (MM) is an incurable plasma cell malignancy often treated by autologous stem cell transplant (ASCT). (haematologica.org)
  • 1 Despite significant therapeutic advances for MM, autologous stem cell transplant (ASCT) following high-dose, single-agent melphalan conditioning remains a cornerstone of therapy. (haematologica.org)
  • A preliminarily study of carcinogenesis and chemosensitivity suggests that this cell model carries a tumor suppressor gene mutation and is sensitive to chemotherapy drugs. (oncotarget.com)
  • Besides CD19 + CD138 − B cells, plasmablasts, and plasma cells were enriched in the tongue of mice colonized with C. albicans suggesting a potential role of B lymphocytes during oral fungal colonization. (frontiersin.org)
  • This has been observed with the human prostatic cancer cell lines JCA-1 and ALVA-31, as well as several thymic cell lines. (the-dna-universe.com)
  • Examples include blood cells and some cancer cells. (coleparmer.com)
  • The compelling conclusion that proliferative exhausted T cells are associated with response in HPV+ head and neck cancer is supported by the cohort of 14 patients with paired tumor and adjacent normal tissue and rigorous bioinformatic analysis of nearly 50,000 single CD3+ T cell transcriptomes. (elifesciences.org)
  • Invading cancer cells can utilize different modes of migration including single cell migration and collective migration. (jcancer.org)
  • Currently, nanobiotechnology is the growing science in the field of medicine employing silver nanoparticles to deliver drugs to target cancer cells and proved to be potential anticancer agents. (ijpsonline.com)
  • moreover, the hedgehog signaling inhibitors, GANT61 and GDC-0449 could reverse the migratory enhancement of BEX2-/- colorectal cancer cells. (ijbs.com)
  • A proliferative exhausted CD8 + T cell cluster (P-Tex) which was beneficial to survival outcomes of patients with HPV-positive HNSCC was identified. (elifesciences.org)
  • It played a vital role in the development of the first polio vaccine and the discovery of telomerase in human cells. (the-dna-universe.com)
  • The injectable polio vaccine developed by Jonas Salk was one of the first products mass-produced using cell culture techniques. (wikipedia.org)
  • The regulatory elements required for this cell-specific gene expression are likely located within its proximal promoter. (molvis.org)
  • The expression profiles of long noncoding RNAs (lncRNAs) and mRNAs of PEX-treated dendritic cells are unknown. (jcancer.org)
  • manifestation in Compact disc4+ cells and adjustable expression in Compact disc8+ cells. (nanoker-society.org)
  • These cells support the growth and maintenance of other cells in culture. (coleparmer.com)
  • Feeder cells can provide essential growth factors, nutrients, and signaling molecules to support the growth of cells that are difficult to culture on their own. (coleparmer.com)
  • They also can control environmental conditions, such as temperature, pH, and oxygen levels, to optimize cell growth and antibody production. (coleparmer.com)
  • When the body needs to make more cells, for example during growth or repair, existing cells divide in two in order to replicate themselves. (elifesciences.org)
  • They can inhibit the growth of tumor cells, kill them via phagocytosis, and promote anti-tumor immunity. (frontiersin.org)