• All clinical strains tested were killed by TTO both as planktonic cells and as biofilms. (nih.gov)
  • You will have access to our biobank of chronic wound isolated S. aureus strains and will assess how these bacteria alter wound healing and immune response using validated histological and immunological methods within the Wilkinson and Caserta groups (at Hull). (findaphd.com)
  • To date, the mechanisms of biofilm formation are not well understood, therefore the purpose of this study was to determine the relationship between the adhesive properties, the level of teichoic acids and the ability to form biofilms of clinical and reference strains of Staphylococcus aureus. (scholarsresearchlibrary.com)
  • In one ICU out of ten, the molecular characteristics, antimicrobial susceptibility profiles and biofilm production of the strains responsible for S. aureus bacteremia were studied. (hal.science)
  • Under static conditions and without exposure to glucose, biofilm production was rare (9.4% of the strains, without any CC398). (hal.science)
  • These infections are often recurrent and increasing numbers are being caused by strains like methicillin-resistant Staphylococcus aureus (MRSA), which are resistant to nearly all known antibiotics. (cmrf.org.nz)
  • The emergence of antibiotic-resistant strains of S. aureus such as methicillin-resistant S. aureus (MRSA) is a worldwide problem in clinical medicine. (cmrf.org.nz)
  • In this study, we analysed the antimicrobial susceptibility patterns of biofilm-producing S. aureus strains isolated from diabetic foot infections. (biomedcentral.com)
  • The scpA gene has a high prevalence in the genome of both commensal- and pathogenic-type S. aureus strains. (wikipedia.org)
  • Any drug impairing crucial processes for bacterial life will inevitably lead to the development of drug-resistant strains, whereas the inhibition of biofilm formation might prevent the onset of bacterial resistance. (intechopen.com)
  • In this section, we will focus on proteins involved in biofilm formation as useful targets for the development of new drugs that can effectively and specifically impair biofilm formation with slight effects on cell survival, thus avoiding the generation of drug-resistant strains. (intechopen.com)
  • Thus, an ideal new antibacterial agent, in addition to its activity on drug-resistant strains of bacteria, should also be capable of killing bacteria in biofilms. (databasefootball.com)
  • Since our system doesn't kill ''S. aureus'' but only prevents it from becoming pathogenic, it is less likely to promote the development of resistant strains. (igem.org)
  • The microorganisms used in the study were obtained from the Department of Microbiology and Antibiotics of the Federal University of Pernambuco, one yeast and three bacterial strains: Candida albicans (ATCC 10231), Staphylococcus aureus (ATCC 29213), Pseudomonas aeruginosa (ATCC 27853) and Enterococcus faecalis (ATCC 6057) previously cultured in Agar Nutrient (Difco, Detroit, MI, USA). (bvsalud.org)
  • This study focused on the changes in biofilm activity and related metabolic pathways of S. aureus treated with lactic acid bacteria planktonic CFS (LAB-pk-CFS) and biofilm state (LAB-bf-CFS). (frontiersin.org)
  • However, there have not been many investigations on how pathogenic bacteria are affected by the metabolic properties of planktonic and biofilm probiotics. (frontiersin.org)
  • Here, we used untargeted metabolomics to investigate the potential impacts of planktonic bacteria (LAB-pk-CFS) and biofilm colonies (LAB-bf-CFS) on the metabolism of S. aureus biofilms. (frontiersin.org)
  • Key techniques used within the PhD will include whole genome sequencing (bacteria and phage characterisation), microbiology ( S. aureus culture and AMR profiling), flow cytometry (immune profiling) and histology (assessing host wound healing). (findaphd.com)
  • We applied this strategy to study coagulase (Coa), a secreted protein and a major contributor to the formation of a fibrin network in S. aureus biofilms that protects bacteria from the host immune system and increases attachment to host surfaces. (sdu.dk)
  • [13] The biofilm bacteria can share nutrients and are sheltered from harmful factors in the environment, such as desiccation, antibiotics, and a host body's immune system. (wikipedia.org)
  • They can be found very early in Earth's fossil records (about 3.25 billion years ago) as both Archaea and Bacteria, and commonly protect prokaryotic cells by providing them with homeostasis, encouraging the development of complex interactions between the cells in the biofilm. (wikipedia.org)
  • [8] [5] The first colonist bacteria of a biofilm may adhere to the surface initially by the weak van der Waals forces and hydrophobic effects. (wikipedia.org)
  • During biofilm formation the bacteria sense a range of environmental cues and interpret these as signals to cluster. (cmrf.org.nz)
  • Once S. aureus adheres to host tissues, it can form biofilms, which enable its persistence by allowing bacteria to evade host defences, impeding access to certain types of immune cells, such as macrophages, which display incomplete penetration into the biofilm matrix and "frustrated phagocytosis" [ 7 ]. (biomedcentral.com)
  • S. aureus expresses the intracellular inhibitor staphostatin A, specific against staphopain A. As the activation of staphopain A could occur before it has been secreted by the bacteria, the staphostatin acts as prevention against harmful intracellular activity of the protease. (wikipedia.org)
  • Staphopain A participates in S. aureus self-regulatory events, by altering the phenotype of the bacteria via cleavage of surface proteins and by preventing biofilm formation. (wikipedia.org)
  • In this study, the anti-biofilm activity of previously fabricated polyamino-phenolic ligands and polyamidoamine dendrimers was investigated against legionella mono-species and multi-species biofilms formed by L. pneumophila in association with other bacteria that can be found in tap water ( Aeromonas hydrophila , Pseudomonas aeruginosa , Escherichia coli , Klebsiella pneumoniae ). (frontiersin.org)
  • [ 4 ] Within 2 weeks, the bacteria within the tubing and bag have had ample opportunity to create a biofilm, providing a safe haven against most antibiotics. (medscape.com)
  • Bacteria cause most boils, with Staphylococcus aureus (staph) being the type that most commonly lead to boils. (medicalnewstoday.com)
  • Biofilm is a complex matrix consisting of extracellular polysaccharides, DNA, and proteins that protect bacteria from a variety of physical, chemical, and biological stresses allowing them to survive in hostile environments. (intechopen.com)
  • Bacteria can be found in planktonic form or in specific conditions, as sessile aggregates on both biotic and abiotic surfaces originating complex structures known as biofilm. (intechopen.com)
  • The biofilm matrix consists of polysaccharides, proteins, and DNA and constitutes a stubborn source that protects bacteria from a variety of physical, chemical, and biological stresses. (intechopen.com)
  • The most common strategies employed by bacteria for resisting antibiotics include growing as biofilms, persisters, and small colony variants [1]. (databasefootball.com)
  • These structures are called biofilms and are thought to resemble the physiological state in which bacteria grow in many natural environments. (databasefootball.com)
  • Importantly, bacteria are capable of forming biofilms in infected human tissues. (databasefootball.com)
  • The biofilm-associated infections caused by even drug-sensitive bacteria are very difficult to treat, and patients tend to have recurrent infections [2]. (databasefootball.com)
  • However, bacteria present in biofilms can evade the antibacterial effect, forming a reservoir of infection. (databasefootball.com)
  • The surviving bacteria from biofilms can spread to various organs causing a relapse of disease. (databasefootball.com)
  • The recalcitrance of bacteria in biofilms has been attributed to the impermeable nature of the biofilm matrix and to the presence of non-growing or very slowly-growing populations of bacteria called persisters. (databasefootball.com)
  • The resistance of biofilm-growing bacteria to antibiotics does not involve any genetic changes but is only a phenotypic adaptation. (databasefootball.com)
  • We have [https://2009.igem.org/Team:SDU-Denmark/Background#Quorum-quenching_using_RIP reason to hope] that this will weaken the biofilm formation, thereby making multiresistant bacteria easier to treat with conventional antibiotics. (igem.org)
  • While the silver infused surface on its own would not be particularly effective at getting rid of S. aureus , by adding a tiny current with a square wave voltage resulted in truly preventing bacteria from growing on the surface. (medgadget.com)
  • Under a high magnification, this scanning electron microscopic (SEM) image depicted numbers of Staphylococcus aureus bacteria, which were found on the luminal surface of an indwelling catheter. (cdc.gov)
  • This biofilm has been found to protect the bacteria that secrete the substance from attacks by antimicrobial agents such as antibiotics. (cdc.gov)
  • Quantitative measurement of biofilm production by the Microtitre plate method (MTP) will be performed for in clinical isolates of bacteria. (who.int)
  • Other combinations of Medihoney and antibiotic produced an interesting array of effects: Medihoney-fusidic acid treatment showed minor synergistic activity, and Medihoney-clindamycin, -gentamicin, and -oxacillin combinations showed overall antagonistic effects when the honey was used at sub-inhibitory concentration, due to enhanced biofilm formation at these concentrations which could not be counteracted by the antibiotics. (umf.org.nz)
  • Staphylococcus aureus is a major human pathogen that utilises many surface-associated and secreted proteins to form biofilms and cause disease. (sdu.dk)
  • OP, the ability to form biofilms and above was also determined at the level (1,0683 ± 0,006) un. (scholarsresearchlibrary.com)
  • Bacterial ability to form biofilms was verified using a crystal violet colorimetric assay and testing cell viability by real-time quantitative PCR and Plate Count assay. (frontiersin.org)
  • Thus, the aim of this research was to determine whether yeast present in CVC colonizations previously exposed to cell- wall targeted antibacterials benefit from a reduction in susceptibility to fluconazole and voriconazole, facilitating their ability to form biofilms. (bvsalud.org)
  • None of the antibacterials exerted a significant effect on the in vitro susceptibility of the yeasts to the antifungal agents or on their ability to form biofilms. (bvsalud.org)
  • These findings show that LAB-CFS has a strong potential to combat S. aureus infections. (frontiersin.org)
  • One of the most prevalent foodborne infections, Staphylococcus aureus ( S. aureus ), is highly dangerous and endangers both human and animal health ( 1 ). (frontiersin.org)
  • The capacity of S. aureus to build biofilms is correlated with the severity of infections ( 4 ). (frontiersin.org)
  • Bacterial biofilms are the major cause of chronic wound infections and are notoriously recalcitrant to treatments with antibiotics, making them difficult to eradicate. (umf.org.nz)
  • In addition, manuka honey exhibits anti-biofilm activity, thereby warranting the investigation of its potential as a combination therapy with antibiotics for the topical treatment of biofilm-related infections. (umf.org.nz)
  • Here we report the first use of MacSynergy II to investigate the response of established S. aureus (strain NCTC 8325) biofilms to treatment by combinations of Medihoney (medical grade manuka honey) and conventional antibiotics that are used for preventing or treating infections: rifampicin, oxacillin, fusidic acid, clindamycin, and gentamicin. (umf.org.nz)
  • Staphylococcus aureus is an opportunistic pathogen related to a variety of life-threatening infections but for which antimicrobial resistance is liming the treatment options. (nature.com)
  • Staphylococcus aureus is an important human opportunistic pathogen involved in a wide range of human infections. (nature.com)
  • Conclusions: A combination of HC and IHC can be used to diagnose and characterise biofilm infections on a routine basis. (ku.dk)
  • Here, we investigated the effect of PYED-1 on the biofilms of Staphylococcus aureus, an etiological agent of biofilm-based chronic infections such as osteomyelitis, indwelling medical device infections, periodontitis, chronic wound infections, and endocarditis. (unina.it)
  • These results indicated that PYED-1 may have great potential as an effective antibiofilm agent to prevent S. aureus biofilm-associated infections. (unina.it)
  • Candida albicans and Staphylococcus aureus , representing two different kingdoms, are the most frequently isolated pathogens from invasive infections. (mdpi.com)
  • New Zealand has among the highest reported incidence of infections with the bacterium Staphylococcus aureus in the developed world. (cmrf.org.nz)
  • This study aims to make inhibitors of the bacterial enzymes that make menaquinone to study how menaquinone affects biofilm formation which will lead to drugs to target and treat S. aureus infections and biofilms. (cmrf.org.nz)
  • Although S. aureus usually acts as a commensal of the human microbiota it can also become an opportunistic pathogen, being a common cause of skin infections including abscesses, respiratory infections such as sinusitis, and food poisoning. (cmrf.org.nz)
  • Staphylococcus aureus is by far the most frequent species isolated from these infections. (biomedcentral.com)
  • Results demonstrate that very high concentrations of the most used antibiotics in treating diabetic foot infections (DFI) are required to inhibit S. aureus biofilms in vitro, which may explain why monotherapy with these agents frequently fails to eradicate biofilm infections. (biomedcentral.com)
  • The results suggest that the antibiotic susceptibility patterns cannot be applied to biofilm established infections. (biomedcentral.com)
  • S. aureus is an important nosocomial pathogen that can cause several infections such as: bacteraemia, osteomyelitis, skin infections, pneumonia, meningitis and endocarditis. (biomedcentral.com)
  • Several structural and secreted virulence factors play a role in S. aureus infections, which are multifactorial and depend on bacterial adherence and biofilm formation. (biomedcentral.com)
  • Failure of antibiotic treatments is often attributed to formation of bacterial biofilms and abscesses in the mammary gland tissue, which lead to chronic infections that are difficult to eradicate and drive recurrent disease. (ntu.edu.tw)
  • During bacterial infections involving biofilms, most of the existing antibiotics can kill the susceptible, planktonic bacterial population present in various body sites. (databasefootball.com)
  • We now report our seminal findings on the major constituents including terpenes identified in native, historically significant herbal medicinal plant Elder (Sambucus nigra L.) flower and elder berry in particular and their concomitant strong antimicrobial effects exhibited on various nosocomial pathogens notably upon methicillin-resistant Staphylococcus aureus MRSA, recognised globally as a clinically significant pathogen, associated with skin and soft tissue infections. (researchgate.net)
  • Persistent Contamination With MRSA After Skin Infections How long does S. aureus contamination linger in a household after the initial skin infection? (medscape.com)
  • It has been observed that MRSAs are capable of producing biofilms and enterotoxins which pose the risk antibiotic resistance and human Staphylococci toxicity. (ajol.info)
  • In this study, we demonstrate that octanoic acid has broad-spectrum microbicidal activity against MCPs and effectively inhibits S. aureus biofilm formation in milk (>50% inhibition at 3.13 mM). (ntu.edu.tw)
  • The concentration of the chemicals tested as anti-biofilm agents was chosen based on cytotoxicity assays: the highest non-cytotoxic chemical concentration was used for biofilm inhibition assays, with dendrimer concentration 10-fold higher than polyamino-phenolic ligands. (frontiersin.org)
  • The effects of these NPs on Acinetobacter baumannii , Staphylococcus aureus , and Escherichia coli biofilms were studied using quantitative biofilm inhibition and disruption assays, as well as using fluorescence, -scanning electron microscopy, and atomic force microscopy. (dovepress.com)
  • AuNPs also showed biofilm inhibition, with the highest of 98% in S. aureus . (dovepress.com)
  • This is the first report on rapid and efficient synthesis of AgNPs, AuNPs and AgAuNPs from P. zeylanica and their effect on quantitative inhibition and disruption of bacterial biofilms. (dovepress.com)
  • The findings demonstrated that the LAB-CFS treatment considerably slowed Staphylococcus aureus ( S. aureus ) growth and prevented it from forming biofilms. (frontiersin.org)
  • In this study, we determined whether TTO is effective against Staphylococcus aureus in biofilms and how TTO activity is affected by the S. aureus growth phase. (nih.gov)
  • This study is aimed at investigating biofilm and enterotoxin producing capacity of methicillin-resistant Staphylococcus aureus isolated from nostrils of healthy students of Delta State University Oleh campus. (ajol.info)
  • Determination of methicillin-resistant Staphylococcus aureus was carried out with the aid of Oxacillin sensitivity disc to test the presence of penicillin binding protein2a (PBP2a). (ajol.info)
  • The Methicillin-resistant Staphylococcus aureus (MRSA) isolates were screened to determine their biofilm producing ability using crystal binding assay. (ajol.info)
  • Manuka honey has potent broad-spectrum antibacterial activity and has previously shown synergistic activity in combination with antibiotics against common wound pathogens, including Staphylococcus aureus . (umf.org.nz)
  • Most of inflammatory diseases which are caused by Staphylococcus aureus, proceed with the formation of biofilms. (scholarsresearchlibrary.com)
  • Background: Staphylococcus aureus (S. aureus) biofilm has been associated with severe and recalcitrant cases of chronic rhinosinusitis (CRS). (edu.au)
  • Aim: Visualization of Staphylococcus aureus biofilm using histochemical staining and combined histochemistry (HC) and immunohistochemistry (IHC). (ku.dk)
  • What is Staphylococcus aureus? (cmrf.org.nz)
  • Staphylococcus aureus is a Gram-positive, round-shaped bacterium that is a member of the Firmicutes, and it is a usual member of the microbiota of the body, frequently found in the upper respiratory tract and on the skin. (cmrf.org.nz)
  • Staphopain A (EC 3.4.22.48, ScpA, ScpAaur, staphylopain A, staphylococcal cysteine proteinase) is a secreted cysteine protease produced by Staphylococcus aureus. (wikipedia.org)
  • Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms. (bvsalud.org)
  • Staphylococcus aureus is a pathogen that frequently causes mastitis in bovine herds worldwide. (bvsalud.org)
  • Staphylococcus aureus merupakan salah satu bakteri yang dapat membentuk biofilm dan merupakan salah satu penyebab RSK. (ub.ac.id)
  • Pembentukan biofilm Staphylococcus aureus melalui 4 tahap yaitu tahap perlekatan, quorum sensing (QS), maturasi dan pelepasan, QS merupakan tahap paling penting pada proses pembentukan biofilm. (ub.ac.id)
  • Pada Staphylococcus aureus komunikasi diperantarai oleh sinyal mikromolekul autoinducer peptide (AIP). (ub.ac.id)
  • Propolis menghambat pertumbuhan Staphylococcus aureus, Staphylococcus epidermidis, Entero-coccus Sp. (ub.ac.id)
  • Malang Indonesia sebagai QSI pada isolat biofilm Staphylococcus aureus dari RSK berdasarkan penurunan AIP, histidine kinase, ekspresi gen agr dan morfologi biofilm. (ub.ac.id)
  • Pertama, Isolasi dan karakterisasi Staphylococcus aureus dan Eksplorasi dosis EEP Trigona Sp. (ub.ac.id)
  • Malang Indonesia sebagai QSI pada isolat biofilm Staphylococcus aureus dari RSK. (ub.ac.id)
  • Penelitian tahap pertama telah dilakukan isolasi dan karakterisasi Staphylococcus aureus. (ub.ac.id)
  • Kultur Congo Red didapatkan biofilm Staphylococcus aureus berwarna hitam. (ub.ac.id)
  • Tes kepekaan antibiotika Staphylococcus aureus multiresisten terhadap antibiotika. (ub.ac.id)
  • Pada tes ini Staphylococcus aureus resisten terhadap Cefoxitin (FOX), Vancomycin (VA), Oxacillin (OX), Penicillin (P), Erythromycin (E) dan Lincomycin (MY). (ub.ac.id)
  • Treatment of lapine knees infected with Staphylococcus aureus with the antibiotic-eluting UHMWPE led to complete bacterial eradication and the absence of detectable systemic effects. (nature.com)
  • A major mastitis-causing pathogen (MCP) associated with biofilms in bovine mastitis is Staphylococcus aureus. (ntu.edu.tw)
  • Phenolphthalein bisphosphate tetrasodium salt has been used as a component of modified Baird Parker (BP) agar to determine the expression of phosphatases of Staphylococcus aureus ATCC12600. (sigmaaldrich.com)
  • Aim: The present article aims to study and compare the antioxidant activity of aqueous solutions of leaves and flowers of Sambucus nigra L obtained at different exposure times and assess the antibacterial activity of these solutions against Escherichia coli ATCC 8739, Salmonella NCTC 6017, Listeria monocytogenes NCTC 11994, and Staphylococcus aureus ATCC 25093. (researchgate.net)
  • To test the capabilities of this approach, the team applied Staphylococcus aureus to a plastic surface containing embedded silver nanoparticles. (medgadget.com)
  • Global Scale Dissemination of ST93: A Divergent Staphylococcus aureus Epidemic Lineage That Has Recently Emerged From Remote Northern Australia. (cdc.gov)
  • Staphylococcus aureus , followed by coagulase-negative staphylococci, Streptococcus pneumoniae and Klebsiella pneumoniae . (who.int)
  • All the MRSA isolates tested for the presence of biofilm showed positive results. (ajol.info)
  • 0,05) in clinical isolates of S. aureus compared to the referent. (scholarsresearchlibrary.com)
  • Consequently, we can assume that the adhesive properties and increase the level of teichoic acids from clinical isolates of S. aureus is a sign of pathogenicity and virulence, which may affect the ability of microorganisms to biofilm formation. (scholarsresearchlibrary.com)
  • The only antibiotics able to inhibit biofilm eradication on 50 % of isolates were ceftaroline and gentamicin. (biomedcentral.com)
  • The ability of the isolates to form a biofilm was examined using the polystyrene surface of microtiter plates. (bvsalud.org)
  • All 159 S. aureus isolates investigated harboured the cap gene 80 % carried the cap5 gene and 20 % carried the cap8 gene . (bvsalud.org)
  • Of the 159 isolates analysed, 97.5 % were biofilm producers. (bvsalud.org)
  • A significant association between the capsular genotype and phenotype and the amount of biofilm formation was detected cap5/CP5 isolates tended to form more biofilm and to produce a thinner CP layer than cap8/CP8 isolates. (bvsalud.org)
  • METHODS: S. aureus and CNS, isolated from 15 minced meat and 15 chicken drumstick samples, were analyzed by conventional biochemical tests and API ID 32 Staph (Bio Merieux SA, Marcy-I'Etoile, France) identification kits to identify for genus and species of these isolates. (turkhijyen.org)
  • Biofilm formation of isolates that was investigated by using Congo Red agar method. (turkhijyen.org)
  • A total of 41 CNS isolates isolated from chicken samples whereas, S. aureus were not isolated from these samples. (turkhijyen.org)
  • All of S. aureus (100 %) and 58 % of CNS isolates from minced meat and 70.7 % of CNS isolates from chicken samples were positive for DNase activity, respectively. (turkhijyen.org)
  • In this study, % 50 of S. aureus and % 18 of CNS from minced and 41.5 % of CNS isolates from chicken samples produced biofilm, respectively. (turkhijyen.org)
  • In this study, we showed that a large proportion of isolates had biofilm production and DNase activity. (turkhijyen.org)
  • Several current aspects related to the virulence of S. aureus and CoNS are discussed, including isolates of different origins, phenotypic and genotypic techniques for the detection of these toxins, and the gene regulation mechanism involved in the expression of these toxins. (novapublishers.com)
  • The minimum biofilm eradication concentration was usually two times higher than the minimum bactericidal concentration, yet it was never higher than 1% v/v. The fastest killing of biofilm occurred during the first 15min of contact with TTO and was not influenced by increasing TTO concentration above 1% v/v. Planktonic stationary phase cells exhibited decreased susceptibility to TTO compared with exponential phase cells. (nih.gov)
  • The antibiotic minimum inhibitory concentration (MIC) was determined for ten antimicrobial compounds, along with the minimum biofilm inhibitory concentration (MBIC) and minimum biofilm eradication concentration (MBEC), followed by PCR identification of genetic determinants of biofilm production and antimicrobial resistance. (biomedcentral.com)
  • Gram-positive organisms, for example, Streptococcus pyogenes and S. aureus , historically were major causes of morbidity and mortality following penetrating trauma in the pre-antibiotic era and remain initial pathogens for many infection types. (medscape.com)
  • Drinking water pipe biofilms control microbial regrowth and are a possible source of pathogens. (who.int)
  • Dental plaque biofilm may promote oral and oropharyngeal colonization of respiratory pathogens in hospitalized subjects. (who.int)
  • The microorganisms in biofilms composed of 50-500 times increase their resistance to disinfectants, antimicrobials, bacteriophages, phagocytes and antibodies. (scholarsresearchlibrary.com)
  • A biofilm comprises any syntrophic consortium of microorganisms in which cells stick to each other and often also to a surface. (wikipedia.org)
  • Biofilms can attach to a surface such as a tooth or rock, and may include a single species or a diverse group of microorganisms. (wikipedia.org)
  • The formation of a biofilm begins with the attachment of free-floating microorganisms to a surface. (wikipedia.org)
  • Disinfection methods currently used in water systems have been shown to be ineffective against legionella over the long-term, allowing recolonization by the biofilm-protected microorganisms. (frontiersin.org)
  • Using checkerboard microdilution assays, viability assays and MacSynergy II analysis we show that the Medihoney-rifampicin combination was more effective than combinations using the other antibiotics against established staphylococcal biofilms. (umf.org.nz)
  • Microbes form a biofilm in response to a number of different factors, [9] which may include cellular recognition of specific or non-specific attachment sites on a surface, nutritional cues, or in some cases, by exposure of planktonic cells to sub-inhibitory concentrations of antibiotics . (wikipedia.org)
  • In fact, biofilms were resistant to antibiotics at concentrations 10-1000 times greater than the ones required to kill free-living or planktonic cells. (biomedcentral.com)
  • Additionally, biofilm cells display increased tolerance to antibiotics [ 8 ]. (biomedcentral.com)
  • In contrast, antibiotics treated at >100X MBC cannot eradicate biofilms but do induce SCVs and antibiotic-tolerant cells. (ntu.edu.tw)
  • Moreover, octanoic acid acts additively with antibiotics against S. aureus, and it attenuates tetracycline-induced virulence factor gene expression in S. aureus cells. (ntu.edu.tw)
  • The poorly metabolizing persisters in the biofilms can thus evade the action of these antibiotics. (databasefootball.com)
  • This study aims to develop a sinonasal tissue explant model to analyze the interaction of S. aureus biofilm with the mucosa in vitro. (edu.au)
  • Methods: Sinonasal tissue samples from 5 control patients undergoing pituitary surgery were cultured with and without S. aureus biofilm in vitro. (edu.au)
  • Methods: The ability of S. aureus S54F9 to form biofilm was tested in vitro. (ku.dk)
  • Results: S. aureus S54F9 showed an ability to form biofilm in vitro. (ku.dk)
  • The aim of the present study was to test for the presence of genes involved in capsular polysaccharide production and biofilm formation in S. aureus isolated from bovine mastitis samples collected from 119 dairy herds located in three different Brazilian regions, as well as to assay the production of capsular polysaccharides and biofilm , in vitro . (bvsalud.org)
  • These data show that TTO efficiently kills S. aureus in the stationary growth phase and within biofilms and is therefore a promising tool for S. aureus eradication. (nih.gov)
  • Octanoic acid effectively clears biofilms (95% eradication at 1X minimum bactericidal concentration, MBC) and infrequently induces S. aureus small colony variants (SCVs) that may cause recurrent mastitis. (ntu.edu.tw)
  • [2] [3] The cells within the biofilm produce the EPS components, which are typically a polymeric conglomeration of extracellular polysaccharides , proteins , lipids and DNA . (wikipedia.org)
  • Twenty-five different histochemical staining protocols were tested in order to find the stains that could identify extracellular biofilm matrix. (ku.dk)
  • Protocols with an optimal visualization of biofilm extracellular matrix were combined with an immunohistochemical protocol based on a specific antibody against S. aureus. (ku.dk)
  • Visualization of biofilm, i.e. bacterial cells and extracellular matrix in different colours, was seen when the immunohistochemical protocol was combined with Alcian Blue pH3, Luna and Methyl-pyronin green. (ku.dk)
  • Coagulase-negative staphylococci can colonize the surface of catheters and produce an extracellular polysaccharide that leads to the formation of biofilms which potentiate their pathogenicity. (novapublishers.com)
  • Thus, octanoic acid exhibits bactericidal action against S. aureus biofilms, and it is less likely than antibiotic therapy to induce persistent cells and pathogen tolerance. (ntu.edu.tw)
  • The microbial community is also a natural source of metabolites and has the potential to be used to create antimicrobial and anti-biofilm agents. (frontiersin.org)
  • OBJECTIVE: In this study, it was aimed to evaluate calf minced meat and chiken drumsticks samples purchased from different supermarkets in Ankara, Turkey for the presence of coagulase positive staphylococci (CPS) and coagulase negative staphylococci (CNS) and to determine their biofilm production and DNase activity. (turkhijyen.org)
  • For this project, you will undertake an interdisciplinary programme of work supervised by Dr Holly Wilkinson (Hull York Medical School), Dr Stefano Caserta (Hull York Medical School) and Dr Paul Fogg (University of York) to elucidate the effects of antimicrobial resistant (AMR) S. aureus on human wound repair, alongside screening the efficacy of novel phage-derived antimicrobial therapies using cutting-edge wound infection models. (findaphd.com)
  • Biofilm growth plays an important role during infection by providing defence against several antagonistic mechanisms. (biomedcentral.com)
  • In the beginning of an infection, S. aureus produces numerous surface proteins, called "microbial surface components recognizing adhesive matrix molecules" (MSCRAMM) that mediate adherence to host tissues. (biomedcentral.com)
  • These effects may accelerate the transition from biofilm to chronic infection. (ntu.edu.tw)
  • S. aureus produces large biofilm formations that support its pathogenicity and confer protection and subsequently drug resistance ( 5 ). (frontiersin.org)
  • The present findings reveal the potential of Myr as an alternative multi-target antivirulence candidate to control S. aureus pathogenicity. (nature.com)
  • The results indicate a high potential for pathogenicity among S. aureus isolated from bovine milk collected from three different regions in Brazil . (bvsalud.org)
  • Additionally, it inhibits the physiological traits of the S. aureus biofilm, including hydrophobicity, motility, eDNA, and PIA associated to the biofilm. (frontiersin.org)
  • Confocal scanning laser microscopy confirmed that different honey-antibiotic combination treatments could eradicate biofilms. (umf.org.nz)
  • The differentially expressed genes and proteins linked to S. aureus biofilms have been identified using transcriptomic and proteomic investigations ( 14 - 17 ). (frontiersin.org)
  • Our findings demonstrate that msfGFP is a good candidate fluorescent reporter to consider when studying proteins secreted by the Sec pathway in S. aureus. (sdu.dk)
  • Transcriptional analysis performed on the established biofilm revealed that PYED-1 downregulates the expression of genes related to quorum sensing (agrA, RNAIII, hld, psm, and sarA), surface proteins (clfB and fnbB), secreted toxins (hla, hlb, and lukD), and capsular polysaccharides (capC). (unina.it)
  • In conclusion, PYED-1 not only effectively inhibited biofilm formation, but also eradicated preformed biofilms of S. aureus, modulating the expression of genes related to quorum sensing, surface and secreted proteins, and capsular polysaccharides. (unina.it)
  • Medihoney and rifampicin were strongly synergistic in their ability to reduce both biofilm biomass and the viability of embedded S. aureus cells at a level that is likely to be significant in vivo . (umf.org.nz)
  • This information is crucial for the clinical diagnosis, care, and prevention of S. aureus . (frontiersin.org)
  • Bacterial biofilms have significant clinical relevance due to their potential to cause resistance to antimicrobial therapy and host defenses. (encyclopedia.pub)
  • In particular, methicillin-resistant S. aureus (MRSA) has emerged as a major clinical and epidemiological problem in hospitals. (biomedcentral.com)
  • In clinical settings, biofilms are often found growing on various medical devices such as catheters implanted in patients. (databasefootball.com)
  • The microbial cells growing in a biofilm are physiologically distinct from planktonic cells of the same organism, which, by contrast, are single cells that may float or swim in a liquid medium. (wikipedia.org)
  • Quorum sensing Inhibitor (QSI) merupakan salah satu pendekatan strategis untuk pengembangan terapi baru pengobatan biofilm bakteri. (ub.ac.id)
  • On the other hand we know that a higher concentration of ''RIP'' is more efficient in quorum-quenching ''S. aureus'', and it will therefore be more efficient if the plasmid is transcribed at all times. (igem.org)
  • Thus, new approaches are required to combat biofilms in chronic wounds. (umf.org.nz)
  • Our results suggest that honey has potential as an adjunct treatment with rifampicin for chronic wounds infected with staphylococcal biofilms. (umf.org.nz)
  • Chronic Rhinosinusitis, S. aureus Biofilm and Secreted Products" Encyclopedia , https://encyclopedia.pub/entry/24220 (accessed November 30, 2023). (encyclopedia.pub)
  • Survival of the Slimiest: The Persistent Nature of Biofilms Biofilms are hardier than we imagined, playing an underappreciated role in nosocomial transmission. (medscape.com)
  • In addition, these devices promote colonization by providing a biofilm surface for bacterial adhesion as well by producing mucosal irritation. (medscape.com)
  • PYED-1 caused a strong reduction in biofilm formation in a concentration dependent manner. (unina.it)
  • A biofilm usually begins to form when a free-swimming bacterium attaches to a surface. (wikipedia.org)
  • Legionnaires' disease is a potentially fatal pneumonia caused by Legionella pneumophila , an aquatic bacterium often found within the biofilm niche. (frontiersin.org)
  • Additionally, octanoic acid rapidly kills persistent biofilm cells and cells with antibiotic tolerance (within 4 h). (ntu.edu.tw)
  • Confocal scanning laser microscopy (CSLM) using the Live/Dead BacLight stain and histology were performed on the tissue explants after 24 hours of biofilm challenge. (edu.au)
  • IL-6 mRNA transcript levels were 4.9-fold upregulated in biofilm-treated tissue compared to controls (p = 0.0485). (edu.au)
  • Conclusion: The sinonasal tissue explant is a viable and functional model capable of analyzing direct biofilm-mucosal interactions and can advance our understanding of the role played by S. aureus biofilm in sinus inflammation. (edu.au)
  • This pore-forming toxin has been reported as an important protein that mediates tissue damage promoted by S. aureus . (nature.com)
  • Those patients often have nasal polyps with tissue eosinophilia, S. aureus -dominant mucosal biofilm, comorbid asthma, and a severely compromised quality of life. (encyclopedia.pub)
  • Hereafter, infected bone tissue was collected from two different porcine models of osteomyelitis inoculated with S. aureus strain S54F9. (ku.dk)
  • AMAÇ: Bu çalışmada Ankara ilindeki, çeşitli süpermarketlerden temin edilen dana kıyma ve tavuk but örneklerinde S. aureus ve koagülaz negatif stafilokok (KNS)'ların bulunma sıklığı ile bu izolatların biyofilm üretimi ve DNaz aktivitelerinin saptanması amaçlanmıştır. (turkhijyen.org)
  • Biofilms are an ensemble of microbial cells irreversibly associated with a surface and enclosed in an essentially self-produced matrix. (intechopen.com)
  • By fusing msfGFP to signal peptides for the Secretory (Sec) and Twin Arginine Translocation (Tat) pathways, the two major secretion pathways in S. aureus, we quantified msfGFP fluorescence in bacterial cultures and cell-free supernatant from the cultures. (sdu.dk)
  • Measurements of IL-6, at both the messenger RNA (mRNA) level (using quantitative reverse-transcriptase polymerase chain reaction [qRT-PCR]) and the protein level (using enzyme-linked immunosorbent assay [ELISA]), were undertaken to evaluate biofilm-mucosa interaction. (edu.au)
  • We report here that myricetin, but not its glycosylated form, can remarkably decrease the production of several S. aureus virulence factors, including adhesion, biofilm formation, hemolysis and staphyloxanthin production, without interfering with growth. (nature.com)
  • Biofilm formation requires three different stages: cell attachment to a solid substrate, adhesion, and growth. (intechopen.com)
  • A clorina CHL-OH-A possibilitou uma fotoinativação maior dos biofilmes de E. coli (39 e 62%), E. faecalis (79 e 92%) e multiespécies (48 e 72%) enquanto que a CHL-Ph-A para o biofilme de S. aureus resistente à meticilina (53 e 89%) nos dois comprimentos de onda utilizados (660 e 403 nm), respectivamente. (usp.br)
  • Potencializar os efeitos fotodinâmicos com iodeto de potássio (KI)foi crucial para a obtenção de menores índices de sobrevivência bacteriana chegando a 100% de erradicação para E. faecalis e S. aureus resistente à meticilina. (usp.br)
  • Conclusions: PDT was effective in reducing the number of viable cells in the studiedmicroorganisms, especially E. faecalis and S. aureus. (bvsalud.org)
  • While Macrophen and Double Macrophen were the most active substances among polyamino-phenolic ligands, dendrimers were overall twofold more effective than all other compounds with a reduction up to 85 and 73% of legionella and multi-species biofilms, respectively. (frontiersin.org)
  • Subpopulations of cells within the biofilm differentiate to perform various activities for motility, matrix production, and sporulation, supporting the overall success of the biofilm. (wikipedia.org)