• ATTO 610, ATTO 611X, ATTO 620, ATTO 633, ATTO 637, ATTO 647 and ATTO 647N are fluorescent labels for the red spectral region. (eurogentec.com)
  • ATTO 647N is an alternative to Cy® 5 and Alexa Fluor® 647. (eurogentec.com)
  • The characteristic features of ATTO 647N are strong absorption, extraordinary high fluorescence quantum yield and outstanding pH and photo-stability. (eurogentec.com)
  • ATTO 647 and ATTO 647N are excited most efficiently in the range 615 - 660 nm. (eurogentec.com)
  • ATTO ® 488, ATTO ® 647N and ATTO ® 594 dyes are suitable for St imulated E mission D epletion ( STED ) microscopy which allows higher resolution imaging compared to confocal laser scanning microscopy. (sysy.com)
  • Both panels include myTags human chromosome 17 centromere probes [ATTO 647N] and chromosomes [stained with Hoescht 33342] pseudo-colored gray. (arborbiosci.com)
  • We report ATACSs of five red to far-red organic dyes, ATTO 647N, STAR 635P, silicon rhodamine, ATTO 594, and ATTO 590. (abberior.rocks)
  • Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, Alexa Fluor® 488. (clickchemistrytools.com)
  • ATTO 520 and ATTO 532 are fluorescent labels related to the well-known dye Rhodamine-6G. (eurogentec.com)
  • ATTO 550 is a fluorescent label related to the well-known dyes Rhodamine-6G and Rhodamine B is the commercial alternative to NED. (eurogentec.com)
  • ATTO 565, ATTO 590 and ATTO 594 are fluorescent labels belonging to the class of Rhodamine dyes. (eurogentec.com)
  • Description: ATTO 488 is a rhodamine-based fluorescent label. (bioinfolab.org)
  • Description: ATTO 532 is a rhodamine-based fluorescent labeling dye with high molecular high extinction coefficient and fluorescence quantum yield (0.90) as well as sufficient Stoke's shift (excitation maximum 532 nm, emission maximum 553 nm). (bioinfolab.org)
  • Description: ATTO 647 is rhodamine-based fluorescent label for the red spectral region that has similar spectral properties to Cy5. (bioinfolab.org)
  • Description: ATTO 514 is a rhodamine-based fluorophore characterized by exceptional water solubility, strong absorption, high fluorescence quantum yield, and outstanding thermal and photo-stability. (bioinfolab.org)
  • Description: ATTO 532 is a rhodamine-based fluorescent labeling dye known for its favorable spectral qualities. (bioinfolab.org)
  • Description: ATTO 647 is a rhodamine-based fluorescent label offering spectral properties similar to Cy5. (bioinfolab.org)
  • Description: ATTO 594 is a rhodamine-based fluorescent label known for its excellent water solubility. (bioinfolab.org)
  • R0, from two dyes excitation/emission spectres. (pymolwiki.org)
  • AZDye™ 488 Maleimide is a green-fluorescent, water-soluble dye with pH insensitive emission from pH 4 to pH 10. (clickchemistrytools.com)
  • ATTO is a trademarks of ATTO-TEC GmbH, Siegen/Germany. (sysy.com)
  • ATTO Dyes are a series of fluorescent labels and dyes manufactured by ATTO-TEC GmbH in Siegen, Germany. (genelink.com)
  • ATTO™ 633 is a red wavelength dye. (idtdna.com)
  • For STED microscopy, this dye can be efficiently used with a STED laser wavelength between 590 - 610 nm. (abberior.shop)
  • The dyes were found to have large ATACSs (>100 GM) at their respective wavelength peaks, thus supporting their use as bright fluorescent markers in TPE microscopy. (abberior.rocks)
  • This product or portions thereof is manufactured under license from ATTO-TEC GmbH. (sysy.com)
  • ATTO 594 exhibits excellent water solubility, very good stability over a wide pH range, very good photo-stability, and high fluorescence quantum yield. (eurogentec.com)
  • ATTO 633 is an alternative to Alexa Fluor 633, ATTO 633 shows strong absorption, very high fluorescence quantum yield, and very good stability in the pH-range 2 to 10. (eurogentec.com)
  • ATTO™ 633 exhibits strong absorption, high fluorescence quantum yield, and good stability in a pH range of 2-10. (idtdna.com)
  • All dyes are fully characterized and can be used in time resolved spectroscopy, FRET (Fluorescence Resonance Energy Transfer) Real-Time qPCR assays applications as well as single- molecule detection. (eurogentec.com)
  • Dye calibration may be required for each commercial detection instrument. (idtdna.com)
  • For many of the fluorescence labeled antibodies conjugated to ATTO ® dyes from ATTO-TEC the established fluorescence detection systems can be used. (sysy.com)
  • A new series of Atto dyes are now available that are are designed for high sensitivity applications, including single-molecule detection. (genelink.com)
  • NHS Ester of Fluorescent Dyes enable one-step labeling & detection of both proteins (e.g. antibodies) via targeting of lysine and any other primary amine-containing macromolecules. (stratech.co.uk)
  • Description: ATTO 594 NHS ester is a derivative of ATTO 594 that can be used to fluorescently label proteins and other amine-containing molecules. (bioinfolab.org)
  • This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. (alomone.com)
  • abberior STAR GREEN is a novel fluorescent dye belonging to the group of fluorescein dyes. (abberior.shop)
  • Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. (sysy.com)
  • Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-NMDAR2A (GluN2A) (extracellular)-ATTO Fluor-488 Antibody (#AGC-002-AG), (1:80). (alomone.com)
  • Immunohistochemical staining of perfusion-fixed frozen mouse and rat brain sections using Anti-K V 4.2-ATTO Fluor-633 Antibody (#APC-023-FR), (1:60). (alomone.com)
  • Conventional and popular dyes that are derivatives of fluoroscein (FAM, HEX and TET) and Cyanine dye derivatives (Cy3, Cy5, Cy5.5, Cy7 etc) are commonly used for fluorescently labeling oligos for use as molecular probes for real time PCR, FISH analysis and fragment analysis. (genelink.com)
  • Maleimide is the most popular sulfhydryl-reactive reactive group for conjugating the dye to either a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. (clickchemistrytools.com)
  • ATTO-TEC dyes can be used as fluorescent labels for a large variety of biomolecules including oligonucleotides. (eurogentec.com)
  • ATTO-TEC fluorescent labels are ideal for many bio-analytical applications. (eurogentec.com)
  • ATTO 725 together with ATTO 740 are fluorescent labels for the near infrared spectral region. (eurogentec.com)
  • Since the start up in 1999 ATTO-TEC is committed to being a first class provider of novel fluorescent labels that advance bioanalytical and biomedical research all over the world. (hum-molgen.org)
  • Such excellent reaction rate constants are unparalleled by any other bioorthogonal reaction pair described to date.Cy®3 is a bright, water-soluble, and pH insensitive orange-fluorescent dye that can be excited using the 532 nm or 555 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. (rx-8.com)
  • The molecules of most common dyes, e.g. cyanines, have a more or less flexible structure. (genelink.com)
  • (Picolyl-) Azides of fluorescent dyes can be used for the fluorescent labeling of terminal Alkyne and strained Alknye (e.g DBCO)-labeled molecules via Cu(I)-catalyzed Alknye-Azide (CUAAC) or Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC) reaction, respectively. (stratech.co.uk)
  • Alkyne-containing Fluorescent dyes can be used for the fluorescent labeling of Azide-tagged molecules via a Cu(I)-catalyzed Azide-Alkyne Click Chemistry reaction (CuAAC). (stratech.co.uk)
  • PALM and FPALM were developed using photoactivatable or photoconvertible fluorescent proteins (tandem dimer Eos and photoactivatable GFP, PA-GFP) as the switchable probes, whereas STORM was originally published using the synthetic carbocyanine dyes, Cy3 and Cy5, to label short DNA molecules. (fsu.edu)
  • The absorption and fluorescence of some dyes are optimized for the red region of the spectrum, with the advantage of efficiently suppressing or by-passing any auto-fluorescence of biological samples. (eurogentec.com)
  • Other dyes exhibit a large shift of fluorescence relative to the absorption (Stokes-Shift). (eurogentec.com)
  • ATTO 725 shows strong absorption and fluorescence. (eurogentec.com)
  • ATTO 740 is another near-infrared emitting fluorophores.Its features are strong absorption and acceptable fluorescence. (eurogentec.com)
  • Other fluorescent dyes are available as N-hydroxysuccinimide (NHS) for conjugation using a primary amine group linked to the oligos. (genelink.com)
  • Description: This ATTO 594 acid can be conjugated to biomolecules with activation by EDC or other catalysts. (bioinfolab.org)
  • ATTO 594 is an alternative to Alexa Fluor® 594. (eurogentec.com)
  • ATTO 637 is another alternative to Alexa Fluor 633. (eurogentec.com)
  • Some of the most cited dyes are the Alexa Fluor dyes from the company invitrogen . (pymolwiki.org)
  • Andy Fluor™ 568 can be used as a replacement for Alexa Fluor 568, ATTO 565. (mobitec.com)
  • This label is analogous to the dyes Alexa 647, Alexa 633 and Cy5 and can be used for direct flow cytometry (FACS) using the He:Ne laser. (alomone.com)
  • abberior STAR GREEN can substitute dyes like Oregon Green 488, ATTO 488 or Alexa Fluor 488. (abberior.shop)
  • Compared with other labels especially for the red region of the spectrum, ATTO-labels show excellent photostability and brightness. (genelink.com)
  • Characteristic features of this dyes are bright green fluorescent with a fluorescent quantum yield of over 80% and exceptional photostability under STED and confocal imaging conditions. (abberior.shop)
  • When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. (thermofisher.com)
  • Suitable excitation sources for ATTO 637 are the 633 nm line of the He:Ne Laser or a Diode-Laser emitting at 635 nm. (eurogentec.com)
  • A suitable excitation source for these dyes is the Krypton-Ion laser using the 676 nm line or a Diode-Laser emitting at 670 nm. (eurogentec.com)
  • C. Wu, K. Mino, H. Akimoto, M. Kawabata, K. Nakamura, M. Ozaki and Y. Ohmiya, In vivo far-red luminescence imaging of a biomarker based on BRET from Cypridina bioluminescence to an organic dye. (atto.co.jp)
  • ATTO ® 488 and ATTO ® 594 are also recommended for PALM and dSTORM high resolution microscopy. (sysy.com)
  • However, these values are not available for recently developed dyes, which exhibit superior properties in many modern microscopy applications. (abberior.rocks)
  • Andy Fluor™ 568 is a bright, red fluorescent dye with excitation ideally suited for the 568 nm laser line. (mobitec.com)
  • This dye is ideally suited to the 488 nm laser line. (clickchemistrytools.com)
  • Standard Dyes such as Cy3, Tamra or Texas Red have been thoroughly selected to cover the whole UV-Vis spectrum. (stratech.co.uk)
  • Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company. (thermofisher.com)
  • Comparing myTags synthetic probes (a) versus BAC-derived probes (b) for the human TP53 locus [ATTO 550] demonstrates that the myTags probes only hybridize to the intended locus, while the BAC-derived probes also display multiple off-target hybridizations, denoted by asterisks in (b). (arborbiosci.com)
  • The ATTO Dye series covers a spectral range from 390 nm in the UV to 740 nm in the near infrared allowing excitation with most commonly used light sources. (genelink.com)
  • Our current stuff brings together long-time expertise in dye chemistry and physics. (hum-molgen.org)
  • Kappa2=2/3 can be a valid fast approximation, for the purpose of ordering dyes. (pymolwiki.org)
  • A spectrally unique, 405 nm-excitable green dye. (biotium.com)
  • Changes in 5-10 % is normal, but much larger values can be expected if the dye make hydrophobic interactions with the protein. (pymolwiki.org)
  • In the literature, I often see this equal 2/3=0,667 This corresponds for free diffusing dye. (pymolwiki.org)