Atherosclerosis bone marrow transplantation in mice. Medical search. Frequent questions

Methods and Results-Bone marrow cells from Ly49A transgenic and nontransgenic littermates were used to repopulate the hematopoietic system of lethally-irradiated female ldl-r -/- mice. (uky.edu)
Accumulated data indicate that hematopoietic stem cell transplantation may be effective under optimal conditions in preventing the progression of central nervous system symptoms in neuronopathic forms of lysosomal storage diseases (such as Krabbe disease), including some of the mucopolysaccharidoses, oligosaccharidoses, sphingolipidoses, and lipidoses as well as peroxisome disorders such as X-linked adrenoleukodystrophy. (medscape.com)
Some evidence indicates that at least in certain disorders, combination ERT and hematopoietic stem cell transplantation together might be superior to hematopoietic stem cell transplantation alone in patients who are appropriate candidates. (medscape.com)
The availability of both ERT and hematopoietic stem cell transplantation has prompted ongoing consideration of newborn screening efforts to diagnose lysosomal storage diseases. (medscape.com)
Hematopoietic Stem Cell Transplantation in Refractory Crohn's Disease: Should It Be Considered? (mdpi.com)
Accumulated data indicate that hematopoietic stem cell transplantation may be effective under optimal conditions in preventing the progression of central nervous system symptoms in neuronopathic forms of lysosomal storage diseases, including some of the mucopolysaccharidoses, oligosaccharidoses, sphingolipidoses, and lipidoses. (medscape.com)

Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
They demonstrated that transferring functional mitochondria of bone marrow-derived stem cells to defective parenchymal cells increases the aerobic respiration capacity of recipient mitochondria ( 7 ). (frontiersin.org)
Experimental and human studies with intravitreal bone marrow-derived stem cells have begun. (biomedcentral.com)
We treat acute myocardial infarction by targeting exogenous bone marrow-derived stem cells (expressing CD45) or endogenous CD34-positive cells to injured cardiomyocytes (expressing myosin light chain. (regenerativemedicine.net)
The strain is now valued as a source of embryonic stem cells for making knockout mice. (jax.org)
Durham, NC (November 24, 2020) - A new treatment for atherosclerosis, commonly known as hardening of the arteries, may be on the horizon, according to a study released today in STEM CELLS Translational Medicine (SCTM) . (stemcellsportal.com)
MSCs are stem cells that can be isolated from bone marrow, adipose and other tissue sources. (stemcellsportal.com)

Analysis of extracellular matrix composition revealed prominent accumulation of versican, a presumed proatherogenic matrix component abundant in human lesions but almost absent in the widely-used C57BL/6 murine atherosclerosis model. (scite.ai)
Methods: Nine-week-old male SAMP10 mice were randomly assigned to a non-YBMT group (n = 6) and a YBMT group (n = 7) that received the bone marrow of 8-week-old C57BL/6 mice. (nagoya-u.ac.jp)
Using a pair of black mice from the Granby farm, Little develops the C57BL and C57BR strains. (jax.org)

We also discussed the advantage and challenges of mitochondrial transfer strategies, including cell-based mitochondrial transplantation, extracellular vesicle-based mitochondrial transplantation, and naked mitochondrial transplantation, for the treatment of cardiovascular disorders. (frontiersin.org)
The abnormal morphology and dysfunction of mitochondria have been proven as the principal mechanisms in the pathogenesis of cardiovascular diseases, such as heart failure, myocardial infarction, atherosclerosis, and hypertension ( 4 - 6 ). (frontiersin.org)
Hypoimmune induced pluripotent stem cell-derived cell therapeutics treat cardiovascular and pulmonary diseases in immunocompetent allogeneic mice. (ucsf.edu)
Since EOCs are a type of endothelial progenitor cell that contributes to vascular repair, this makes them a promising candidate for treating atherosclerosis and other cardiovascular diseases, too. (stemcellsportal.com)

3 4 Since then, it has become evident that MSCs reside in many tissues, including mesenchymal tissues (bone, adipose tissue, connective tissue), umbilical cord and several organs including the liver, spleen and lung. (bmj.com)

Although longitudinal natural history data are limited, published guidelines are available to assist with decisions related to the pursuit of transplantation and whether to use bone marrow or umbilical cord blood-derived cells. (medscape.com)

Inflammation and immunity are key factors for the development and complications of atherosclerosis, and therefore, the whole atherosclerotic process is a target for diagnosis and treatment. (hindawi.com)
Therefore, with the aim to summarize the current knowledge on the initiation of the atherosclerotic process, in this paper, we review the early markers of atherosclerosis and we address the main therapeutic targets for preventing atheroma formation at its very initial stages focusing on inflammation, oxidative stress, endothelial dysfunction, and the interaction between platelets and endothelium. (hindawi.com)
Mast cells play an important role in a variety of biological processes including allergic reactions, atherosclerosis and inflammation 7,8 . (bvsalud.org)

Conclusions: These findings suggest that YBMT can prevent muscle wasting and dysfunction by mitigating apoptosis and proliferation via a modulation of GDF-11 signalling and mitochondrial dysfunction in SAMP10 mice. (nagoya-u.ac.jp)
Moreover, Mitofilin is revealed to be indispensable for mitochondrial homeostasis and osteogenesis of BMMSCs, and that insufficiency of Mitofilin leads to BMMSC senescence and bone loss. (thno.org)

Hypoimmune anti-CD19 chimeric antigen receptor T cells provide lasting tumor control in fully immunocompetent allogeneic humanized mice. (ucsf.edu)
METHODS@#A physical exercise intervention of swimming (30 min/d, 5 d/week, for 4 weeks) was administered to BALB/c nude mice bearing subcutaneous human HCC tumor. (bvsalud.org)
RESULTS@#Swimming significantly reduced the body weight and tumor weight in nude mice bearing HCC tumor. (bvsalud.org)

To determine this role, we created chimeric atherosclerosis-susceptible low-density lipoprotein (LDL) receptor null (ldl-r -/- ) mice that were deficient in functional NK cells through expression of a transgene encoding for Ly49A. (uky.edu)
Here we identify the interleukin (IL)-33 and its receptor ST2 as key negative regulators of wound healing and permeability in the colon of mice. (bmj.com)
The study demonstrates how injecting mice with early outgrowth cells (EOCs), after first treating the cells with chemicals to activate a type of protein called liver x receptor (LXR), slows the development of this disease. (stemcellsportal.com)

2 MSCs were first described in the bone marrow where they constitute a small fraction of cells (0.001%-0.01%) that closely interact with haematopoietic cells to support haematopoiesis and skeletal homeostasis. (bmj.com)
Here, we intend to investigate whether RESV counteracts senescence-associated bone loss via osteogenic improvement of MSCs and the underlying mechanism. (thno.org)
MSCs derived from bone marrow (BMMSCs) and the bone-specific, senescence-accelerated, osteoblastogenesis/osteogenesis-defective mice (the SAMP6 strain) were used as experimental models. (thno.org)

Human hypoimmune primary pancreatic islets avoid rejection and autoimmunity and alleviate diabetes in allogeneic humanized mice. (ucsf.edu)

When the normal function of the endothelium is disrupted by mechanical trauma, hypertension, hypercholesterolemia, or atherosclerosis, less EDRF is released, and the inhibition of platelet aggregation is decreased. (unboundmedicine.com)

Despite being identified in human and mouse atherosclerotic lesions, the role of NK cells in the disease process in unknown. (uky.edu)
Using immunohistochemistry, we detected NK cells in atherosclerotic lesions of both groups of mice. (uky.edu)

Enriched monolayer precursor cell cultures from micro-dissected adult mouse dentate gyrus yield functional granule cell-like neurons. (mdc-berlin.de)

A mouse genetics approach can simplify the genetic analysis and provide tools for mechanistic studies. (scite.ai)
Key Words: atherosclerosis Ⅲ congenic strain Ⅲ genetics Ⅲ extracellular matrix Ⅲ mapping S usceptibility to atherosclerosis is influenced by both genetic and environmental factors, with approximately 40% to 60% of interindividual variation attributed to genetic factors. (scite.ai)
The Jackson Laboratory has made fundamental contributions to biomedical research, including cancer genetics and establishing the mouse as the premier research animal model. (jax.org)

Methods and Results-We previously identified atherosclerosis susceptibility QTL (Athsq1) on chromosome 4 acting independently of systemic risk factors. (scite.ai)
MATERIALS AND METHODS: An AS mouse model was developed with ApoE-/- mice fed a high-fat diet (HFD). (bvsalud.org)
METHODS@#Thirty New Zealand white rabbits were randomly divided into atherosclerosis model group, highfat diet feeding group, and normal chow feeding group (blank control group). (bvsalud.org)

Here, we investigated the benefits and mechanisms of the preventive effects of YBMT on loss of muscle mass and function in a senescence-associated mouse prone 10 (SAMP10) model, with a special focus on the role of growth differentiation factor 11 (GDF-11). (nagoya-u.ac.jp)

Background: Young bone marrow transplantation (YBMT) has been shown to stimulate vascular regeneration in pathological conditions, including ageing. (nagoya-u.ac.jp)
Stem cell transplantation is a promising strategy for therapeutic cardiac regeneration, but current therapies are limited by inefficient interaction between potentially beneficial cells (either exogenously transplanted or endogenously recruited) and the injured tissue. (regenerativemedicine.net)
In this article, Mr. Steve Hill highlights research on selective bone regeneration using a protein called Jagged-1. (transhumanist-party.org)
Today, we would like to highlight a recent study in which researchers show a way to selectively accelerate bone regeneration. (transhumanist-party.org)

Atherosclerosis starts with dysfunctional changes in the endothelium induced by disturbed shear stress which can lead to endothelial and platelet activation, adhesion of monocytes on the activated endothelium, and differentiation into proinflammatory macrophages, which increase the uptake of oxidized LDL (oxLDL) and turn into foam cells, exacerbating the inflammatory signalling. (hindawi.com)
After 8 weeks, there was no difference in either serum total cholesterol concentrations or lipoprotein cholesterol distribution in mice repopulated with nontransgenic versus Ly49A transgenic marrow cells. (uky.edu)
However, deficiency of functional NK cells significantly reduced the size of atherosclerosis by 70% (P=0.0002) in cross-sectional analysis of the aortic root and by 38% (P=0.004) in en face analysis of the intimal surface of the aortic arch. (uky.edu)
provides an overview of the dynamic in vivo life of NK cells from their development in the bone marrow to their mature cellular responses in the periphery and their ultimate demise, with emphasis on mouse NK cells and viral infections. (scielo.br)
review the heterogeneity of the current models for memory T cells generation and maintenance in humans and mice. (scielo.br)
In a very interesting review, P. MARRACK & J. KAPPLER focus on naïve, activated, and memory T cells survival separately, and discuss experiments done in vitro and in mice lacking or containing normal populations of lymphocytes. (scielo.br)
SMMC-7721 cells were treated with nude mice serum. (bvsalud.org)
HE staining and IHC results showed a lower necrotic area ratio as well as fewer PCNA or Ki67 positive cells in mice receiving the swimming intervention. (bvsalud.org)
In vitro experiments showed that treating cells with the serum from the swimming intervention mice significantly reduced the formation of SMMC-7721 cell suspension sphere, as well as the mRNA expression level of stemness transcription factors. (bvsalud.org)
UCSF News reports on fetal stem cell transplantation, taking healthy cells from the bone marrow of a donor, and transplanting them into the fetus through ultrasound-guided injections with the goal of having the implanted cells, or graft, replenish the patient's supply of healthy blood-forming cells. (ucsf.edu)

Objective-Susceptibility to atherosclerosis is genetically complex, and modifier genes that do not operate via traditional risk factors are largely unknown. (scite.ai)
1939 International Committee on Standardized Nomenclature for Mice begins, bringing order to the naming of mice and their genes. (jax.org)

We generated mice expressing ABCA1 in macrophages and mice with selected inactivation of ABCA1 in macrophages by bone marrow transplantation in ABCA1-deficient ( ABC1 -/- ) and wild-type (WT) mice. (jci.org)
The results of a bone marrow transplantation experiment suggested that both accelerated lesion development and versican accumulation are mediated, at least in part, by macrophages. (scite.ai)
He was able to obtain mice without the UCP2 gene, demonstrating the essential role of this gene in innate immunity and the limitation of free radical levels, particularly in macrophages in collaboration with Denis Richard at Laval University. (wikipedia.org)

Wild-type and St2 −/− mice were used in wound healing experiments and in two experimental models of IBD triggered by 2,4,6-trinitrobenzene sulphonic acid or dextran sodium sulphate (DSS). (bmj.com)
Halsey Bagg develops the BALB /c ( Bagg albino) mouse for behavioral experiments. (jax.org)

They have achieved this by delivering Jagged-1 to injuries instead of the bone morphogenetic proteins (BMPs) that have been traditionally used. (transhumanist-party.org)

At all times, the level of HDL in ABC1 -/- recipient mice remained low relative to WT recipient mice irrespective of the genotype of the donor macrophage ABCA1 or high-fat feeding. (jci.org)

1 The complex etiology has hampered genetic studies of atherosclerosis in humans per se until recently. (scite.ai)
In a very interesting issue, R.J. NORTH & Y-J. JUNG first argue that mouse tuberculosis and tuberculosis in guinea pig and rabbit are models of tuberculosis in susceptible humans. (scielo.br)
In contrast, the rodents treated with BMPs benefited but also experienced problematic bone hypertrophy, which is also observed in humans using BMPs. (transhumanist-party.org)

Researchers in the UK and Denmark reduced cancerous tumors by 1/3 in mice and rats with lab induced cancers. (blogspot.com)
Many studies have been performed that involve animal models of spinal cord injury (mostly rats and mice), but these are limited in scale by financial, practical, and ethical considerations. (regenerativemedicine.net)
Mice and rats that were given Jagged-1, applied using a wet collagen sponge, saw improvements to both femoral and skull injuries. (transhumanist-party.org)

Applications of the work include metabolic diseases (obesity, diabetes), nutrition, degenerative diseases and autoimmune diseases involving oxygenated free radicals in atherosclerosis and neurodegeneration. (wikipedia.org)
In general, transplantation yields the best results when performed early in the course of the disease (ie, in an asymptomatic affected sibling of a child with a lysosomal storage disorder), in centers with experience in performing transplantations to treat inherited metabolic disorders, and in patients healthy enough to tolerate the conditioning and transplantation regimen. (medscape.com)

Here we apply targeted nanomedicine to achieve in vivo cell-mediated tissue repair, imaging and localized enrichment without cellular transplantation. (regenerativemedicine.net)

Indeed, DPPI deficient mice were protected against acute arthritis induced by passive transfer of monoclonal antibodies against type II collagen (Adkison et al. (justia.com)
In two experimental models of IBD, genetic ablation of ST2 significantly improved signs of colitis, while a sustained epithelial expression of the cyto-protective factor connexin-43 was observed in DSS-treated St2 -deficient mice. (bmj.com)

After a recovery period to permit sufficient engraftment, mice were placed on a diet enriched in saturated fat and cholesterol. (uky.edu)

Cell therapy with young bone marrow from green fluorescent protein (GFP) transgenic mice exhibited GFP+ myofibres in aged muscle tissues. (nagoya-u.ac.jp)

Atherosclerosis is a chronic vascular inflammatory disease associated to oxidative stress and endothelial dysfunction. (hindawi.com)
Chronic intermittent application of RESV enhances bone formation and counteracts accelerated bone loss, with RESV improving osteogenic differentiation of senescent BMMSCs. (thno.org)

Detects mouse Serum Amyloid A1/SAA1 and mouse Serum Amyloid A2/SAA2 in direct ELISAs and Western blots. (rndsystems.com)
This antibody functions as an ELISA detection antibody when paired with Rat Anti-Mouse Serum Amyloid A1/A2 Monoclonal Antibody (Catalog # MAB2948 ). (rndsystems.com)
Detection of Mouse Serum Amyloid A1/A2 by Western Blot. (rndsystems.com)
Western blot shows mouse serum and mouse plasma. (rndsystems.com)
PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Serum Amyloid A1/A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2948) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017 ). (rndsystems.com)
Western blot shows recombinant mouse Serum Amyloid A1 and recombinant mouse Serum Amyloid A2. (rndsystems.com)
Detection of Mouse Serum Amyloid A1/A2 by Simple Western TM . (rndsystems.com)
Simple Western lane view shows mouse serum, loaded at a 1:100 dilution. (rndsystems.com)
Mouse Serum Amyloid A1/A2 ELISA Standard Curve. (rndsystems.com)
Recombinant Mouse Serum Amyloid A1/A2 protein was serially diluted 2-fold and captured by Rat Anti-Mouse Serum Amyloid A1/A2 Monoclonal Antibody (Catalog # MAB2948 ) coated on a Clear Polystyrene Microplate (Catalog # DY990 ). (rndsystems.com)
Goat Anti-Mouse Serum Amyloid A1/A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2948) was biotinylated and incubated with the protein captured on the plate. (rndsystems.com)

In the after-math of the bombings of Hiroshima and Nagasaki in 1945, researchers discovered that bone marrow (BM) transplanted into irradiated mice produced hematopoiesis [ 1 ]. (biomedcentral.com)
The researchers led by Kurt Hankenson , D.V.M., Ph.D., a professor of orthopedic surgery at Michigan Medicine, hypothesized for some years that by binding Jagged-1 to a biomaterial structure and delivering it to the site of injury, it could improve healing of the bone. (transhumanist-party.org)

Oxidation of low-density lipoprotein (LDL) cholesterol is one of the key factors for the development of atherosclerosis. (hindawi.com)
Oxidation of low-density lipoprotein (LDL) cholesterol is crucial in the development of atherosclerosis, and low LDL levels reduce the risk of major events in patients with CVD [ 6 ]. (hindawi.com)

Lathrop sends mice that developed tumors to Leo Loeb at the University of Pennsylvania , who publishes pioneering papers on cancer. (jax.org)

Chuanxiong) are both traditional Chinese medicines with vascular protective effects, which help detoxify and activate blood, and are clinically used to treat atherosclerosis (AS). (bvsalud.org)

Finally, M. BUSSLINGER focuses on the transcription factors that regulate early B-lymphopoiesis in the bone marrow. (scielo.br)
William Castle opens Harvard 's Bussey Institution , where many early mouse geneticists get their start. (jax.org)

Malaria-specific antibody responses and parasite persistence after infection of mice with Plasmodium chabaudi chabaudi. (mdc-berlin.de)

Jagged-1 is an osteoinductive protein that activates the Notch signaling pathway, which regulates bone healing at the site of injury. (transhumanist-party.org)

Atherosclerosis is a leading cause of heart attack, stroke and other cardiopulmonary disorders. (stemcellsportal.com)

Interestingly, comparative mapping revealed that the Athsq1 congenic interval contains the mouse region homologous to a widely-replicated CHD locus on human chromosome 9p21. (scite.ai)

Immunologist Qizhi Tang, Ph.D., professor and director of the Department of Surgery's Transplantation Research Lab and Tang Lab, is one of the featured speakers at the first annual UCSF Immuno"XX": Women in Immunology Symposium on October 22, 2018 and will lead the session on Tolerance and Immunity. (ucsf.edu)

Expression of WT macrophage ABCA1 in ABC1 -/- mice resulted in a small but significant increase in apoA-I levels starting 2 weeks after transplantation. (jci.org)

Bone mass, bone formation rates and osteogenic differentiation of BMMSCs were primarily evaluated. (thno.org)
Peter Gorer shows in mouse studies at JAX that transplant rejection is primarily governed by what he calls the H2 genetic locus, later described as the major histocompatibility complex, a key component of immunity. (jax.org)

The new technique avoids the issues of inappropriate or excessive bone growth because, unlike BMPs, it targets osteoinductive mechanisms that are more directly associated with the regenerative process. (transhumanist-party.org)

The increase in apoAI was accompanied by a small but significant increase in HDL cholesterol 6 weeks after transplantation. (jci.org)

More recently, studies in mice with targeted inactivation of ABCA1 ( 11 ) have demonstrated a critical role of ABCA1 in lipid metabolism and confirmed its role in the biogenesis of HDL. (jci.org)

Tuberculosis in mice is also exclusively a lung disease that is progressive and lethal, in spite of the generation of Th1- mediated immunity. (scielo.br)

This function of UCP2 has been confirmed by the demonstration of a protective role of UCP2 against atherosclerosis. (wikipedia.org)

First successful transplantations of ovaries between female mice are performed by William Russell at JAX. (jax.org)

The findings of this study suggest that the use of Jagged-1 for location-specific bone injury could potentially be developed into a therapy to help people recover from fractures and broken bones. (transhumanist-party.org)

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