Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was employed to characterize the polygalloyl polyflavan-3-ols (PGPF) in grape seed extracts. (nih.gov)
In recent years matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has emerged as a tool for microbial identification and diagnosis. (matis.is)
Evaluation of the Andromas matrix-assisted laser desorption ionization-time of flight mass spectrometry system for identification of aerobically growing Gram-positive bacilli. (pasteur.fr)
Matrix-associated laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is a rapid and simple microbial identification method. (pasteur.fr)
Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) is widely used in clinical laboratories for routine identification of bacteria and yeasts. (knaw.nl)
Dive into the research topics of 'A New Filter Based Cultivation Approach for Improving Aspergillus Identification using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS)'. Together they form a unique fingerprint. (knaw.nl)
A New Filter Based Cultivation Approach for Improving Aspergillus Identification using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) . Mycopathologia , 187 (1), 39-52. (knaw.nl)
Dive into the research topics of 'Efficient identification of Malassezia yeasts by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)'. Together they form a unique fingerprint. (uab.cat)
Efficient identification of Malassezia yeasts by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) . British Journal of Dermatology , 170 , 332-341. (uab.cat)
Ma-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been reported to be a rapid and reliable diagnostic tool to identify clinically important yeasts, but so far no data have been reported on identification of Malassezia isolates with this technique. (uab.cat)
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been developed for the rapid identification of microorganisms and proved to be a robust tool for the recognition of bacterial species. (uab.cat)
Although the matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) systems are relatively less studied, they have been reported to be a promising method for the identification of anaerobes. (elsevierpure.com)
Is the Subject Area "Matrix-assisted laser desorption ionization time-of-flight mass spectrometry" applicable to this article? (plos.org)
Identifying fungal isolates using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. (cdc.gov)
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used for species determination. (lu.se)
Cell proteins were analysed with 2-dimensional electrophoresis and differentially expressed proteins were identified by matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry. (who.int)
Two-dimensional differential gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) based proteomics was used to study differential protein abundance in various B cell types during B cell maturation (cell lines are listed in Supplementary Table 1 ). (lu.se)
MALDI12
Matrix-assisted laser desorption electrospray ionization (MALDESI) was first introduced in 2006 as a novel ambient ionization technique which combines the benefits of electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI). (wikipedia.org)
The original MALDESI design was implemented using common organic matrices, similar to those used in MALDI, along with a UV laser. (wikipedia.org)
Here we report the first application of a matrix-assisted laser desorption/ionization (MALDI) triplequadrupole mass spectrometer for targeted proteomics. (canada.ca)
The technique was then applied to validate an important virulence biomarker of the fungal pathogen Candida albicans, which was not accurately quantified using global proteomics experiment employing two-dimensional liquid chromatography/electrospray ionization tandem mass spectrometry (2D-LC/ESI)-MS/MS. Using LC/MALDI-MRM analysis of five tryptic peptides, the protein PHR1 was found to be upregulated in the hyphal (pathogenic) form of C. albicans by a factor of 7.7W0.8. (canada.ca)
The use of glycerol as a matrix for oligonucleotide and DNA ionization by infrared matrix-assisted laser desorption/ionization (MALDI) has been the subject of much interest in recent years. (lsu.edu)
The goal of the work described below is to improve the performance of IR MALDI with a glycerol matrix by mixing the glycerol with a solvent that does not absorb the mid-IR laser radiation. (lsu.edu)
Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, and its associated analytic software, was recently approved for clinical microbiology diagnostic use by the United States Food and Drug Administration. (temple.edu)
A method based on the analyte-to-matrix ion abundance ratio in the matrix-assisted laser desorption ionization (MALDI) spectrum was used for the quantification of phosphatidylcholine (PC) in mouse brain tissue. (kribb.re.kr)
Herein, we report that breast cancer (BC) patients can be distinguished from cancer-free (NC) controls by serum immunoglobulin G (IgG) crystallizable fragment (Fc) region N-glycosylation profiling using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Recently, there has been much progress in the field of tumor immunology. (elsevierpure.com)
Tri-alpha-napthyl benzene (TANB) is used as a matrix in matrix-assisted laser desorption/ionization (MALDI) spectroscopy. (nist.gov)
We used 3 different approaches: biochemical testing, matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry, and 16S ribosomal RNA (rRNA) gene sequencing. (medscape.com)
This presentation will also address biosafety and other considerations when identifying bacterial select agents and toxins using Matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (MS). (cdc.gov)
Surface-assisted laser desorption2
Surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) is ideally suited for the high-throughput analysis of small molecules in bodily fluids (e.g. saliva, urine, and blood plasma). (bsz-bw.de)
In this study, we propose a simple and rapid technique for characterization of free fatty acids and triacylglycerides (TAG) based on palladium nanoparticular (Pd-NP) surface-assisted laser desorption/ionization (SALDI) mass spectrometry (MS). The implemented Pd-NP material allowed detection of free fatty acids and TAGs exclusively as [M + K]+ ions in positive ion mode. (tib.eu)
Mass12
The experimental settings involved in the communication between the sampling stage, mass spectrometer and laser desorption, such as stage height, ESI-Spot distance and laser repetition rate, have undergone progressive optimization by several design of experiments (DOE). (wikipedia.org)
Matrix-Assisted Laser Desorption Ionization-Mass Spectrometry Signal E" by N Rodthongkum, YB Chen et al. (umass.edu)
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (rush.edu)
This graph shows the total number of publications written about "Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization" by people in this website by year, and whether "Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization" was a major or minor topic of these publications. (rush.edu)
Below are the most recent publications written about "Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization" by people in Profiles. (rush.edu)
Malik, MI, Trathnigg, B & Saf, R 2009, ' Characterization of ethylene oxide - propylene oxide block copolymers by combination of different chromatographic techniques and matrix-assisted laser desorption ionization time-of-flight mass spectroscopy ', Journal of Chromatography A , vol. 1216, pp. 6627-6635. (elsevierpure.com)
Modern flight-capable mass spectrometers, in combination with various sample processing, separation, and ionization techniques enable sensitive detection of chemical biosignatures. (frontiersin.org)
Neutral Per- and Polyfluoroalkyl Substances in In Situ Landfill Gas by Thermal Desorption-Gas Chromatography-Mass Spectrometry. (acs.org)
To this end database search engines were rapidly developed after the introduction of ionization techniques for biological mass spectrometry [1-5]. (lu.se)
Analysis of carbohydrates and glycoconjugates by matrix-assisted laser desorption/ionization mass spectrometry: An update for 2017-2018. (bvsalud.org)
Absorbs2
The term 'matrix' refers to any molecule that is present in large excess and absorbs the energy of the laser, thus facilitating desorption of analyte molecules. (wikipedia.org)
The matrix absorbs the laser energy, thus inducing a soft disintegration of the sample-matrix mixture into free (gas phase) matrix and analyte molecules and molecular ions. (rush.edu)
Molecules1
Analyte molecules are embedded in an excess matrix of small organic molecules that show a high resonant absorption at the laser wavelength used. (rush.edu)
Ultraviolet2
An infrared (IR) or ultraviolet (UV) laser can be utilized in MALDESI to resonantly excite an endogenous or exogenous matrix. (wikipedia.org)
Each sample is irradiated by 240 brief pulses from an ultraviolet nitrogen laser (337 nm), desorbed from the plate into the gas phase and ionized. (matis.is)
Negative ionization2
In a study to improve the detection of tissue-specific lipids, the electrospray parameters have been tailored for positive and negative ionization polarities. (wikipedia.org)
Under negative ionization conditions, unusual trimetric structures were generated for free fatty acids, while TAGs underwent irreproducible degradation reactions. (tib.eu)
Wavelength1
Under the proper conditions, double-stranded DNA in excess of 2000 bases in length can be ionized using lasers operating in the 3 µm wavelength region of the mid-IR. (lsu.edu)
Chemistry1
Research group of Kermit Murray at Louisiana State University where we use lasers for sampling and imaging and study the chemistry and physics of laser ablation. (lsu.edu)
Matrix2
The current MALDESI source employs endogenous water or a thin layer of exogenously deposited ice as the energy-absorbing matrix where O-H symmetric and asymmetric stretching bonds are resonantly excited by a mid-IR laser. (wikipedia.org)
Nursing Central , nursing.unboundmedicine.com/nursingcentral/view/Tabers-Dictionary/737987/all/matrix_assisted_laser_desorption_ionization. (unboundmedicine.com)
Detection2
The ESI-like ionization mechanism has been experimentally demonstrated and studied in depth, showing equivalent softness as ESI and enabling the detection of intact protein complexes. (wikipedia.org)
Here, we show that vertically-aligned ordered silicon nanopillar (SiNP) arrays fabricated using nanosphere lithography followed by metal-assisted chemical etching (MACE) are suitable substrates for the SALDI-MS detection of methadone and small peptides. (bsz-bw.de)
Analysis1
Over the past ten years, transformative developments in the laser technology, data acquisition, motor-control software (RastirX), and imaging processing software (MSiReader) have promoted IR-MALDESI as a powerful tool for the direct analysis and MSI of diverse biological, forensic, and pharmaceutical samples. (wikipedia.org)
Mode1
A mid-IR laser that excites the O-H stretching mode of water is used to desorb the neutral materials from the biological samples. (wikipedia.org)
Sample1
A water-cooled Peltier thermoelectric plate is used to control the sample stage temperature (−10 °C to 80 °C). The source has single- or multi-shot capabilities with adjustable laser fluence, repetition rate, and delay between the laser trigger and MS ion accumulation. (wikipedia.org)
Time of Flight Ma18
To decrease the rate misidentifications, an accurate identification method, such as matrix-assisted laser desorption ionization time of flight mass spectrometry or molecular methods, should be used. (hindawi.com)
S. lugdunensis , identified by superior methods, such as the recently described matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry-(MALDI TOF MS-) based identification [ 7 ], could be used to redefine the species of S. lugdunensis and newly investigate their prevalence and pathogenicity. (hindawi.com)
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is a relatively new addition to the clinical microbiology laboratory. (nih.gov)
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry, which has revolutionized the routine identification of microorganisms in clinical microbiology laboratories, was recently successfully applied to the identification of arthropod vectors. (hal.science)
In this study, we summarize the state-of-the-art of matrix-assisted laser desorption ionization time-of-flight mass spectrometry applied to the identification of arthropod vectors (ticks, mosquitoes, phlebotomine sand-flies, fleas, triatomines, lice and Culicoides), their trophic preferences and their ability to discriminate between infection statuses. (hal.science)
본 연구에서는 matrix-assisted laser desorption ionization-time of flight mass spectrometry(MALDI-TOF MS)를 이용한 carbapenemase 검출법에 대한 평가를 시행하였고 기존의 carbapenemase 검출법들과 비교 평가하였다. (dsmc.or.kr)
In this study, the performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was evaluated for the detection of carbapenemase and compared with the results of a modified Hodge test (MHT), a three-dimensional extract (3-D) bioassay, imipenem hydrolysis, and a molecular test. (dsmc.or.kr)
The relative binding strength of a series of terpyridine metal complexes of the type [M(II)L(2)](+) was investigated by using variable laser intensities in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS). (tue.nl)
To evaluate the application of weak cation exchange (WCX) magnetic bead-based Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) in detecting differentially expressed proteins in the urine of renal clear cell carcinoma (RCCC) and its value in the early diagnosis of RCCC. (cams.cn)
Protein fingerprinting using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) provides a rapid, reliable and economical alternative method of characterisation of fungal isolates. (supsi.ch)
In order to establish rapid and accurate typing method for Bacillus coagulans strains which is important for controlling in some canned foods and tea-based beverages manufacturing because of the high-heat resistance of the spores and high tolerance of the vegetative cells to catechins and chemicals, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and repetitive-PCR (rep-PCR) were evaluated. (elsevierpure.com)
Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) is a promising and fast method for identifying fungi and bacteria directly from positive blood cultures. (ku.dk)
Microflex LT (Bruker Daltonics) and VITEK MS (bioMérieux) are bacterial identification systems that are based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). For VITEK MS, two identification softwares, VITEK MS IVD (IVD) and SARAMIS (SARAMIS), are available. (elsevierpure.com)
Identification of bacterial species was performed with matrix-assisted laser desorption/ionization-time of flight mass spectrometry and pneumococci were serotyped using multiplex polymerase chain reactions. (springer.com)
Identifying fungal isolates using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. (cdc.gov)
In this work we identified these protein subunits with the modern proteomic tool of one-dimensional SDS-PAGE and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The three separated pMMO subunits of 45, 27, and 23 kDa were observed on the SDS-PAGE and identified as the expression gene products of the pmoB, pmoC, and pmoA, respectively. (ncl.edu.tw)
On June 24, the hospital laboratory reported an atypical gram-negative isolate from a blood specimen, which was sent that day to a commercial reference laboratory for further identification using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF). (cdc.gov)
Cell proteins were analysed with 2-dimensional electrophoresis and differentially expressed proteins were identified by matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry. (who.int)
Matrix-assisted laser31
Matrix-assisted laser desorption electrospray ionization (MALDESI) was first introduced in 2006 as a novel ambient ionization technique which combines the benefits of electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI). (wikipedia.org)
After tryptic digestion, we performed matrix-assisted laser desorption ionization-imaging MS (MALDI-IMS) experiments in a MALDI-quadrupole time-of-flight mass spectrometer. (nih.gov)
Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) can be used for such determination and its key advantage is the tolerance to salts, detergents and contaminants. (jove.com)
The following is a summary of the " Performance of lipid fingerprint by routine matrix-assisted laser desorption/ionization time of flight for the diagnosis of Mycobacterium tuberculosis complex species ," published in the March 2023 issue of Clinical Microbiology and Infections Disease by Gonzalo , et al. (physiciansweekly.com)
Heat-inactivated mycobacteria cultures were then loaded onto the matrix-assisted laser desorption/ionization target. (physiciansweekly.com)
Matrix-assisted laser desorption/ionization-time of flight had a sensitivity of 94.4 (95% CI, 86.4-98.5) and a specificity of 94.4 (95% CI, 72.7-99.9). (physiciansweekly.com)
This report presents an analytical approach to tissue characterization based on matrix-assisted laser desorption ionization time-of-flight (MALDI TOF) mass spectrometry imaging (MSI) which is introduced in an attempt to develop a reference database for predictive classification of brain tumors. (nih.gov)
Goodwin, R.J. , MacIntyre, L. , Watson, D.G. , Scullion, S.P. and Pitt, A.R. (2010) A solvent-free matrix application method for matrix-assisted laser desorption/ionization imaging of small molecules. (gla.ac.uk)
Matrix application continues to be a critical step in sample preparation for matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI). (gla.ac.uk)
This study examined whether matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) could detect multiply charged dyes of pen inks directly from paper. (ojp.gov)
Targeted analyte detection by standard addition improves detection limits in matrix-assisted laser desorption/ionization mass spectrometry. (nih.gov)
Matrix-assisted laser desorption/ionization (MALDI) has proven an effective tool for fast and accurate determination of many molecules. (nih.gov)
In the last decade, matrix-assisted laser desorption ionization TOF mass spectrometry (MALDI-TOF MS) was introduced as a modern microbiological diagnostic tool and has recently been used routinely as a powerful assay for rapid and highly accurate microbial identification in microbiology laboratories. (medscape.com)
Available applications and workflow of matrix-assisted laser desorption ionization TOF mass spectrometry using different clinical samples. (medscape.com)
Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, and its associated analytic software, was recently approved for clinical microbiology diagnostic use by the United States Food and Drug Administration. (temple.edu)
Meier, MAR, Lohmeijer, BGG & Schubert, US 2003, ' Relative binding strength of terpyridine model complexes under matrix-assisted laser desorption/ionization mass spectrometry conditions ', Journal of Mass Spectrometry , vol. 38, no. 5, pp. 510-516. (tue.nl)
Comparison of the newer techniques with the more established gas chromatography/mass spectrometry and attenuated total reflection Fourier transform infrared spectroscopic imaging shows good agreement between the methods, with each method detecting repeatable differences between the donors, with the exception of matrix-assisted laser desorption ionization, for which quantitative analysis has not yet been established. (northumbria.ac.uk)
Matrix-assisted Laser Desorption Ionization-Time of Flight Mass Spectroscopy (MALDI-TOF MS) has been used routinely over the past decade in clinical microbiology laboratories to rapidly characterize diverse microorganisms of medical importance both at the genus and species levels. (frontiersin.org)
Recently, we combined atmospheric pressure matrix assisted laser desorption (AP-MALDI) with a commercial ion trap mass spectrometer (ITMS) [1]. (nih.gov)
1. High-resolution matrix-assisted laser desorption/ionization imaging of tryptic peptides from tissue. (nih.gov)
2. Protein identification by accurate mass matrix-assisted laser desorption/ionization imaging of tryptic peptides. (nih.gov)
3. Matrix-assisted laser desorption/ionization imaging protocol for in situ characterization of tryptic peptide identity and distribution in formalin-fixed tissue. (nih.gov)
7. Chemically-assisted fragmentation combined with multi-dimensional liquid chromatography and matrix-assisted laser desorption/ionization post source decay, matrix-assisted laser desorption/ionization tandem time-of flight or matrix-assisted laser desorption/ionization tandem mass spectrometry for improved sequencing of tryptic peptides. (nih.gov)
9. Peptides quantification by liquid chromatography with matrix-assisted laser desorption/ionization and selected reaction monitoring detection. (nih.gov)
10. Dansyl-peptides matrix-assisted laser desorption/ionization mass spectrometric (MALDI-MS) and tandem mass spectrometric (MS/MS) features improve the liquid chromatography/MALDI-MS/MS analysis of the proteome. (nih.gov)
11. Comparison of vacuum matrix-assisted laser desorption/ionization (MALDI) and atmospheric pressure MALDI (AP-MALDI) tandem mass spectrometry of 2-dimensional separated and trypsin-digested glomerular proteins for database search derived identification. (nih.gov)
12. Capillary liquid chromatography/atmospheric-pressure matrix-assisted laser desorption/ionisation ion trap mass spectrometry: a comparison with liquid chromatography/matrix-assisted laser desorption/ionisation time-of-flight and liquid chromatography/electrospray ionisation quadrupole time-of-flight for the identification of tryptic peptides. (nih.gov)
16. De novo sequencing of tryptic peptides sulfonated by 4-sulfophenyl isothiocyanate for unambiguous protein identification using post-source decay matrix-assisted laser desorption/ionization mass spectrometry. (nih.gov)
This Matrix Assisted Laser Desorption Ionization - Time of Flight (MALDI-TOF) mass spectrometer was obtained in 2015. (edu.au)
The reaction products obtained were analyzed by matrix assisted laser desorption ionization time-of-flight (MALDI ToF) mass spectrometry. (techscience.com)
Proteins1
Brzhozovskiy A, Kononikhin A, Bugrova AE, Kovalev GI, Schmit PO, Kruppa G, Nikolaev EN, Borchers CH . The Parallel Reaction Monitoring-Parallel Accumulation-Serial Fragmentation (prm-PASEF) Approach for Multiplexed Absolute Quantitation of Proteins in Human Plasma. (mcgill.ca)
Data acquisition1
Over the past ten years, transformative developments in the laser technology, data acquisition, motor-control software (RastirX), and imaging processing software (MSiReader) have promoted IR-MALDESI as a powerful tool for the direct analysis and MSI of diverse biological, forensic, and pharmaceutical samples. (wikipedia.org)
Spectra1
Their method was quick (10 minutes), based on the identification of distinct lipid signatures from the seven reference strains, and yielded interpretable profiles for all but 4 isolates due to poor ionization, for a total of n = 86 with interpretable spectra. (physiciansweekly.com)
Methods1
It is equipped with an ESI/MALDI dual source and has a nanospray (Captivespray nanoBooster source), a home-built offline nanospray source, APCI and APPI (dissolved and solid probe) ionisation methods either via syringe infusion or connection with a Waters Acquity UPLC H-Class with PDA detector. (edu.au)
Source3
The current MALDESI source employs endogenous water or a thin layer of exogenously deposited ice as the energy-absorbing matrix where O-H symmetric and asymmetric stretching bonds are resonantly excited by a mid-IR laser. (wikipedia.org)
A water-cooled Peltier thermoelectric plate is used to control the sample stage temperature (−10 °C to 80 °C). The source has single- or multi-shot capabilities with adjustable laser fluence, repetition rate, and delay between the laser trigger and MS ion accumulation. (wikipedia.org)
Ionisation modes available include ESI, APCI (liquid and direct probe), APPI (liquid and direct probe), online nanospray source (Captivespray nanoBooster source) and a home-built offline nanospray source. (edu.au)