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  • Real-tim
  • Thus, confirmation of primer specificity, one or more invariant endogenous controls, RNA and cDNA quality, as well as real-time PCR amplification efficiencies and linearity calculations from individual slopes or R 2 -values must be included in each study. (mdpi.com)
  • microbial
  • Bioprocessing begins upstream, most often with culturing of animal or microbial cells in a range of vessel types (such as bags or stirred tanks) using different controlled feeding, aerating, and process strategies. (bioprocessintl.com)
  • Sarah began her EPA career 11 years ago as a microbiologist researching novel microbial risk assessment methodologies to support an EPA response to a biological contamination incident. (epa.gov)
  • Assessing
  • Along with identifying common chemical contaminants, differentiating between different types of nucleic acids is also important when assessing sample purity. (qiagen.com)
  • Assessing the purity of nucleic acid samples helps you make informed decisions regarding whether a sample is suitable for processing in the next analysis step or whether it should be discarded from the workflow or reprocessed before further analysis. (qiagen.com)
  • No single technology is capable of assessing all QC parameters within analysis workflows, but together, the QIAxpert , QIAxcel Advanced and PyroMark systems can conveniently cover all your QC needs. (qiagen.com)
  • contaminants
  • With unique spectral content profiling capabilities, the method also provides information about the sample quality, differentiating RNA molecules from DNA and other absorbing contaminants. (qiagen.com)
  • Amino
  • Increasing evidence shows that the intrinsic error rates, and the resulting mutation frequencies, of RNA viruses can be modulated by subtle amino acid changes to the viral polymerase. (jove.com)
  • Phylogenomics (370,877 amino acids) placed H. duospinosa within the Neanuridae. (biomedcentral.com)
  • Methods
  • 2013. Quality control methods for optimal BCR-ABL1 clinical testing in human whole blood samples. (qiagen.com)
  • The results of the study emphasize the need for quality control of the nucleic acid extracts with standardised methods to prevent false negative results and to allow data comparison among different diagnostic laboratories. (pubmedcentralcanada.ca)
  • The World Health Organization (WHO) has developed its first guidelines on testing for chronic HBV and HCV infection, using a framework based on methods from the Grading of Recommendations, Assessment, Development, and Evaluation (GRADE) Working Group for the formulation of recommendations, including determining the strength of recommendations and quality of evidence. (biomedcentral.com)
  • integrity
  • Automated algorithms such as the QIAxcel Advanced RNA Integrity Score (RIS) assess several parameters including the 28S and 18S peaks and attribute a score ranging from 1 (highly degraded) to 10 (mostly intact). (qiagen.com)
  • Objective assessment of RNA integrity using RIS improves comparison of samples, standardization and reproducibility of experiments. (qiagen.com)
  • fatty
  • In this paper, we investigated the effects of dietary n-3 and n-6 fatty acids on the colonic mucosal inflammatory response to DNBS-induced colitis in rats. (jove.com)
  • All rats were fed identical diets with the exception of different types of fatty acids [safflower oil (SO), canola oil (CO), or fish oil (FO)] for three weeks prior to exposure to intrarectal DNBS. (jove.com)
  • Fatty acid profiles of total lipids or specific lipid classes. (giaqua.org)
  • clinical
  • Hence, there is an unmet need for novel biomarkers for more accurate diagnosis, risk assessment, and clinical outcome prediction for both acute and chronic cardiovascular diseases in the elderly. (europa.eu)
  • affects
  • Besides, in Aquaculture production, the nutritional composition of feeds and ingredients is not only critical for aquatic animals growth, but also greatly affects their quality for the consumers. (giaqua.org)
  • To conclude, this study shows that the choice of nucleic acid extraction kit affects the quantity and quality of extracted products. (biomedcentral.com)
  • gene
  • Except for one homemade protocol, the majority gave comparable results in terms of the quality of the extracted DNA measured by the ability to amplify differently sized control gene fragments by PCR. (pubmedcentralcanada.ca)
  • microbiological
  • In addition, this study could assist in the design of new guidelines to improve microbiological quality control in indoor work environments. (biomedcentral.com)
  • Production environments in pharmaceutical companies are kept under strict control to warrant the highest microbiological quality standards. (biomedcentral.com)
  • In the present work, we aimed at developing a novel approach for microbiological quality control of water and air applicable to professional indoor environments, including pharmaceutical companies. (biomedcentral.com)
  • Analysis
  • Quality Control: An Important Success Factor in Nucleic Acid-Based Analysis. (qiagen.com)
  • A conventional analysis of solid materials generally focuses on the specific physical/chemical parameters of the materials. (rsc.org)
  • We show that proper experimental design and analysis parameters can reduce false positives, provide greater resolution of species in complex metagenomic samples, and improve the interpretation of results. (biomedcentral.com)
  • GIAbiochem service offers a wide range of analysis to determine the nutritional quality of these and other alimentary products. (giaqua.org)
  • Quantitative analysis of character economically important (estimate genetic parameters). (giaqua.org)
  • Analysis of immunological parameters in blood and mucus. (giaqua.org)
  • Fast and accurate at-line elemental analysis Built on the experience and success of the proven Epsilon 3 range of XRF spectrometers, the Epsilon 4 is a multi-functional instrument for any industry segment needing elemental analysis from fluorine (F) to americium (Am) in areas from R&D through to process control. (pharmaceutical-business-review.com)
  • Sarah is the Acting Associate Division Director for the Threat and Consequence Assessment Division in the National Homeland Security Research Center, where she manages sampling and analysis development projects and exposure assessment projects. (epa.gov)
  • concentration
  • In reality, the method actually measures all nucleic acids in the sample and can result in overestimation of the RNA or DNA concentration. (qiagen.com)
  • Commercial control sets have also been developed including, The Lucidea Microarray Score Card (Amersham Biosciences) and the SpotReport (Stratagene Inc., La Jolla, CA). These control sets have limited utility for evaluating the parameters in array fabrication, where uniform signal intensity across a given probe concentration is required. (biomedcentral.com)
  • chemicals
  • Regulatory Agencies have clearly established the need to identify and control harmful chemicals which may leach from container closure systems into the final packaged drug product. (contractpharma.com)
  • Sequence
  • The enormous amount of sequence data available in the public domain database has been a gold mine for researchers exploring various themes in life sciences, and hence the quality of such data is of serious concern to researchers. (biomedcentral.com)
  • Vector contamination remains a serious concern to the data quality in the public sequence database nowadays. (biomedcentral.com)
  • LUCY [ 12 ] searches for the greatest number of high quality subsequences in the query sequence according to the quality value of each base, which has been computed by a chromatogram base-calling program, such as phred [ 8 , 9 ], on the trace data. (biomedcentral.com)
  • However, the presence of low-quality bases, sequence minal fragments and filters out substandard reads [ (deepdyve.com)
  • This process goes on for cycles and the signal intensity per flowing nucleotide is recorded for each bead over time and is analyzed to generate good quality sequence. (pubmedcentralcanada.ca)
  • efficiency
  • The real-time efficiency and therefore the output data can be influenced through the kind of calibrator used, the amount and quality of used RNA and by the degree of individual assay variability. (mdpi.com)
  • By conducting nested case-control studies in large repositories of archived prostatic tissue, the efficiency and cost-effectiveness of prognostic studies can be strongly improved, while preserving the validity of a prospective cohort study. (biomedcentral.com)
  • results
  • These results indicate that vB-EcoS-95 is a newly discovered E. coli phage that may be potentially used to control the formation of biofilms. (frontiersin.org)
  • RNA quality control, along with results verification and validation, are critical to ensure that the results can be confidently converted into actionable insights. (qiagen.com)
  • These challenges include evaluation of sample input, library quality and whether project requirements can be met using the data generated from a given set of results. (frontiersin.org)
  • high
  • High-throughput production in any field requires quality metrics. (frontiersin.org)
  • High quality 3' and 5' reads were assembled into 16,578 consensus sequences, 45% of which represented full length inserts. (biomedcentral.com)
  • The service GIAMesocosm offers, both for commercial production and experimental studies, high quality eggs, larvae and juveniles from a wide range of marine species of aquaculture interest such as gilthead sea bream (Sparus aurata), European sea bass (Dicentrarchus labrax), meagre (Argyrosomus regius), sole (Solea sp. (giaqua.org)
  • The same is true for Claisse pre-fused and homogeneous borate fluxes, high-quality platinumware, as well as analytical consulting services. (pharmaceutical-business-review.com)
  • assurance
  • Contribution to quality assurance via proficiency testing and if feasible reference materials in collaboration with the CRL and the network of NRLs connected to it. (europa.eu)
  • The EU and Candidate Countries benefit from the improved quality assurance and the activity is offering training possibilities for scientists in a field were major advances can be expected. (europa.eu)
  • reads
  • After quality filtering, the number of reads ranged 3.851-286.699 (average 76.994 ± 44.781) for water samples, and 1.117-203.107 (average 55.749 ± 59.369) for air samples. (biomedcentral.com)
  • study
  • On completion of this unit students should be familiar with the major concepts and tools of epidemiology, the study of health in populations, and should be able to judge the quality of evidence in health-related research literature. (edu.au)
  • Dinitrobenzene sulfonic acid (DNBS)-induced colitis allows one to study the pathogenesis of IBD associated environmental triggers such as stress and diet, the effects of potential therapies, and the mechanisms underlying intestinal inflammation and mucosal injury. (jove.com)
  • include
  • Com- Existing tools for QC and preprocessing can be divided into plex statistics include the distribution of quality score and base 2 categories according to their structures: toolkit and work- composition distribution for each position. (deepdyve.com)
  • enable
  • UV spectrophotemetry measurements enable calculation of nucleic acid concentrations based on the sample's absorbance at 260 nm. (qiagen.com)
  • Our technologies enable the measurement of parameters such as particle size, shape and zeta potential, biomolecular interactions and stability, rheological properties, elemental concentrations and crystallographic structure. (pharmaceutical-business-review.com)
  • important
  • With continuous innovation in diagnostic instrumentation and techniques, it is more important than ever to have effective, efficient quality control to monitor laboratory assay precision. (bio-rad.com)
  • therefore, it is important to understand choices that may introduce bias or decrease the quality of the data. (biomedcentral.com)
  • types
  • However, the disadvantage of this method is that classical spectrophotometers cannot differentiate between types of nucleic acids. (qiagen.com)
  • products
  • Bio-Rad offers the world's most comprehensive menu of quality control products, the leading QC data management software, and unparalleled customer services. (bio-rad.com)
  • Malvern Panalytical technologies are used by scientists and engineers in a wide range of industries and organizations to solve the challenges associated with maximizing productivity, developing better quality products and getting them to market faster. (pharmaceutical-business-review.com)