Assay development efforts should focus on the incorporation of a robust and optimized experimental design aimed at assessing the sources of variability, repeatability and reproducibility of the assay. (nas.edu)
ISO/TR 22971:2005 provides users with practical guidance to the use of ISO 5725-2:1994 and presents simplified step-by-step procedures for the design, implementation, and statistical analysis of inter-laboratory studies for assessing the variability of a standard measurement method and on the determination of repeatability and reproducibility of data obtained in inter-laboratory testing. (iso.org)
Variability3
Validation from the ELISA confirmed intra- and inter-assay variability of significantly less than 15% and precision of 99.8% ± 8.3% as assessed by spike recovery. (biotech-angels.com)
They then assessed the variability of these data to determine the potential impact on concordance with NAMs. (nih.gov)
Using therapeutic monoclonal antibody-spiked sera and a pooled beta-migrating M-protein, we aimed to assess SPEP limitations and variability across 16 laboratories in three continents. (degruyter.com)
Assays4
Pharmaceutical manufacturers typically use in vitro assays on new encapsulations to assess drug availability, to monitor quality control and/or to fulfill regulatory requirements. (nas.edu)
Limit of blank, limit of detection, linear range, and PCR efficiency were assessed for each of the 12 PCR assays using serial samples dilutions. (biomedcentral.com)
The major part of the total noise (SD 0.14) was caused by the replicate-to-replicate noise of the PCR assays (repeatability) and was not associated with different operating conditions (reproducibility). (biomedcentral.com)
If several bioassays based on the same endpoint (covering a relevant mode of toxic action) are identified and it is not clear what bioassay is best suited to assess this endpoint, different bioassays covering the same mode of action but different stages of the toxicity pathway or involving different visualisations of the effect (e.g., gene reporter assays with different reporter genes) will be included. (watereuse.org)
Precision7
Precision, on the other hand, measures the level of consistency, repeatability, or reproducibility of a set of measurements. (differencedigest.com)
Accuracy relates to how close a measurement is to the true value, while precision relates to the consistency and reproducibility of measurements. (differencedigest.com)
Precision refers to the level of consistency or reproducibility in a set of measurements. (differencedigest.com)
Determining an appropriate level of specificity (discrimination) among heterogeneous botanical matrices as well as evaluating accuracy (recovery) and inter-laboratory precision (reproducibility) within strict and volatile regulatory environments are potential obstacles to the establishment of robust analytical procedures. (researchgate.net)
The test demonstrated 100% precision in inter-day, intra-day, and intra-laboratory reproducibility studies. (fortunejournals.com)
Based on analysis of reference glasses with a range of compositions, the accuracy, precision, and long-term reproducibility of the CU tephra lab's data is excellent. (concord.edu)
For some elements, the current analytical precision exceeds that of most tephra labs which submitted data to the 2011 INTAV interlaboratory comparison (Kuehn et al. (concord.edu)
Analytical2
It also demonstrates the utility of the 4-level panel design to assess the linearity, analytical sensitivity and imprecision of local RT-qPCR methods and achieve precise analytical calibration to the IS across a clinically representative range of BCR-ABL1 expression levels. (asuragen.com)
The RPA assay to detect Leptospira DNA was optimized with Leptospira reference strains and its performance characteristic such as analytical, diagnostics and reproducibility were assessed. (researchsquare.com)
Accuracy1
High Interlaboratory Reproducibility and Accuracy of Next-Generation Sequencing-Based Bacterial Genotyping in a Ring-Trial. (cdc.gov)
Methods5
The overriding question for the use of hair analysis in environmental public health is the need to find reliable methods for assessing chemical exposure of people living in communities near hazardous waste sites. (cdc.gov)
The highest priority research need was to evaluate the wide variety of methods available to detect SARS-CoV-2 genetic material in wastewater and assess their reliability, reproducibility, and sensitivity. (envirosymposium.group)
Subsequently, WRF and the Gates Foundation funded a project to conduct an interlaboratory assessment of 36 methods for SARS-CoV-2 detection in wastewater which evaluated the efficacy of the predominant methods (Pecson et al 2021). (envirosymposium.group)
The paper includes data supporting the stability, reproducibility and compatibility of the synthetic calibrators with various RT-qPCR methods commonly used worldwide in both IS and non-IS standardized laboratories. (asuragen.com)
Regardless of whether fast or slow desorption methods are used to assess the gas content of coal samples, both of these methods have limitations in their ability to accurately determine the gas content of a given sample. (azomining.com)
Assessment2
In the first International Guidelines on TIL-assessment in breast cancer we proposed to develop an inter-laboratory Ring study to assess the reproducibility and clinical validity of TILs assessment, including machine learning algorithms. (tilsinbreastcancer.org)
The reproducibility assessment with the third-party testing laboratory, revealed better agreement on the performance of the assay with a kappa value of 0.81(0.547 to 1.00 at 95% CI). (researchsquare.com)
Experimental2
Boyle, a pioneer of the experimental method, maintained that the foundations of knowledge should be constituted by experimentally produced facts, which can be made believable to a scientific community by their reproducibility. (wikipedia.org)
Two major steps are naturally distinguished in connection with reproducibility of experimental or observational studies: When new data is obtained in the attempt to achieve it, the term replicability is often used, and the new study is a replication or replicate of the original one. (wikipedia.org)
Intra2
Therefore, it is necessary to minimize these variations to eliminate consequential errors and ensure intra- and inter-laboratory reproducibility. (biochemia-medica.com)
Results were assessed for intra- and inter-operator variation, and the effects of cryopreservation. (ox.ac.uk)
Laboratories4
Inter-laboratory reproducibility from the modified HIF-1α ELISA was evaluated at two indie NCI laboratories NCTVL and PADIS with 18 matched up remove examples. (biotech-angels.com)
These results demonstrate the reproducibility of the ELISpot assay across operators and laboratories, and support the use of cryopreserved samples in future BMEM studies. (ox.ac.uk)
Qualification of an RM is a procedure that establishes the numerical or qualitative value of the biological response of the material under specified test conditions, ensuring reproducibility of the response either within laboratories or between laboratories, or both. (freestandardsdocuments.com)
NOTE The use of an RM will facilitate the comparability of the response between laboratories and help assess reproducibility of the test performance within individual laboratories. (freestandardsdocuments.com)
Strict1
and 2) semen smear prepared by Spermac and assessed by strict criteria. (biochemia-medica.com)
Impacts1
The specific project objectives were (A) to review literature and give an overview on bioanalytical tools that can assess likely impacts on human health, (B) to validate novel endpoints and implement them together with existing bioassays for benchmarking water quality of recycled water, and (C) to develop interpretation guidelines. (watereuse.org)
Measurement method1
Typically, the gas content of coal is assessed through a direct measurement method, such as slow or fast desorption. (azomining.com)
Closely1
Reproducibility, closely related to replicability and repeatability, is a major principle underpinning the scientific method. (wikipedia.org)
Comparison3
We conducted an interlaboratory comparison to assess the reproducibility of the measurement of the soil water retention curve. (copernicus.org)
In order to allow you to prepare for the change RCI has performed some comparison studies to allow you to assess the impact that the change will have on the titre endpoint, that we will report following the change. (blood.co.uk)
The suitability of category 2 bioassays to be included into the battery will be assessed with experiments using defined positive and negative controls and with the same samples to which category 1 bioassays were applied, but no wider inter-laboratory comparison will be performed with the category 2 bioassays within the current project. (watereuse.org)
Results3
With a narrower scope, reproducibility has been introduced in computational sciences: Any results should be documented by making all data and code available in such a way that the computations can be executed again with identical results. (wikipedia.org)
In recent decades, there has been a rising concern that many published scientific results fail the test of reproducibility, evoking a reproducibility or replication crisis. (wikipedia.org)
Obtaining the same results when analyzing the data set of the original study again with the same procedures, many authors use the term reproducibility in a narrow, technical sense coming from its use in computational research. (wikipedia.org)
Data1
Because humans are the primary subject of interest for regulatory safety testing, it is advantageous to have human reference data available for evaluation of NAMs for assessing chemical safety. (nih.gov)
Studies2
Assay measurements should incorporate the use of process controls, rigorous statistical analysis techniques and assay robustness evaluations appropriate for the design of interlaboratory studies. (nas.edu)
Ideally, biomarkers can be used to predict disease progression and treatment response, but these studies will take many years due to the requirement for lengthy follow-up periods to assess outcomes. (biomedcentral.com)
Variation1
Cryopreserved samples were shipped to a second laboratory in order to assess inter-laboratory variation. (ox.ac.uk)
Conditions2
However, these criteria can only be applied when sperm analysis is carried out at 37 °C. Sperm motility is highly influenced by temperature and should always be assessed under controlled thermal conditions (5). (biochemia-medica.com)
The ability to measure the gas content within a coal sample allows for mine safety to be assessed during the outburst and/or high gas emission conditions. (azomining.com)
Diagnostic1
Real-Time PCR for Ceratocystis platani detection: in-depth validation to assess the diagnostic potential and include additional technical options. (sisef.it)
Chemicals2
In recent years, the local lymph node assay (LLNA) has emerged as a practical option for assessing the skin-sensitization potential of chemicals. (cdc.gov)
The 2003 EC proposal for the In the USA, laboratory animal use is federally reg- Registration, Evaluation and Authorisation of ulated by the Animal Welfare Act 1966 (amended in Chemicals (REACH), similarly aims to assess the 1985), which excludes laboratory-bred mice and toxicity of chemicals produced or imported in high rats, as well as non-mammals, from consideration quantities (15-20). (drugstorepdfsearch.com)
RESEARCH1
Current genomic and proteomic research provides many candidate biomarkers, but independent validation of these biomarkers is lacking, and reproducibility is still a key concern. (biomedcentral.com)
Biological2
The biological response of the material shall be established through interlaboratory tests. (freestandardsdocuments.com)
One of the first electrochemical models proposed that the cell membrane exerts linear physicochemical changes ( 12 , 13 ) to assess the EMF parameters for which biological effects might be expected. (frontiersin.org)
Risk1
Assessing the Risk of Antibiotic Resistance Transmission from the Environment to Humans: Non-Direct Proportionality between Abundance and Risk. (cdc.gov)
Science2
The first to stress the importance of reproducibility in science was the Irish chemist Robert Boyle, in England in the 17th century. (wikipedia.org)
Such assertions express a common dogma in modern science that reproducibility is a necessary condition (although not necessarily sufficient) for establishing a scientific fact, and in practice for establishing scientific authority in any field of knowledge. (wikipedia.org)
General1
Replicability and repeatability are related terms broadly or loosely synonymous with reproducibility (for example, among the general public), but they are often usefully differentiated in more precise senses, as follows. (wikipedia.org)
Samples1
The MAT assay detects serogroup-specific antibodies in the late acute phase of the disease and needs the seroconversion to be assessed in paired sera samples. (researchsquare.com)