Assays phosphatidylinositol 3 kinases. Medical search. Frequent questions

Activation of phosphatidylinositol-3-kinase (PI3K) and downstream signalling by AKT/mammalian target of rapamycin (mTOR) modulates cellular processes such as increased cell growth, cell proliferation and increased cell migration as well as deregulated apoptosis and oncogenesis. (nih.gov)
Activated IRS-1 directly interacts with and activates phosphatidylinositol-3-kinase (PI3K), promoting PKB/Akt signaling. (nih.gov)
Focal adhesion kinase (FAK)-mediated phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) has been found to be associated with several carcinomas. (ias.ac.in)
Western blot assay was performed to measure the relative protein expressions of phosphorylated (p)-FAK/FAK, p-PI3K/PI3K, p-AKT/AKT, matrix metalloproteinase 9 (MMP9), and ras homolog gene family member A (RhoA), and to determine transfection efficiency. (ias.ac.in)
Activation of the MAPK/ extracellular-signal-regulated kinase (ERK) pathway contributed to the resistance observed in treated mice, while no significant changes were found with the PI3K/Akt pathway and other MAPK pathways. (ox.ac.uk)
The phosphatidylinositol 3-kinase (PI3K) pathway is frequently deregulated in human cancer and is an essential regulator of cellular proliferation. (cancerdiagnosisprognosis.org)
We aimed to determine which tyrosine kinases contribute to resistance elicited by PI3K silencing and inhibition. (cancerdiagnosisprognosis.org)
Results: Activated zeta chain of T-cell receptor-associated protein kinase 70 (ZAP70) generated resistance to PI3K inhibition. (cancerdiagnosisprognosis.org)
The phosphatidylinositol 3-kinase (PI3K) pathway is one of the most activated signaling pathways in human cancer. (cancerdiagnosisprognosis.org)
Upon activation of receptor tyrosine kinases (RTKs), recruitment of PI3K to the cell membrane occurs via coupling the Src homology-2 (SH2) domains of p85 with tyrosine phosphorylated residues of activated receptors ( Nat Rev Drug Discov 8(8) (2009)">4 ). (cancerdiagnosisprognosis.org)
Oncogenic RAS and other tyrosine kinases can cause PI3K activation. (cancerdiagnosisprognosis.org)
Previous studies have identified the contribution of several activated tyrosine or serine/threonine kinases to growth compensation upon PI3K inhibition ( Otorhinolaryngol Head Neck Surg 1(2) (2016)">8 - Oncogene 30(22) (2011)">10 ). (cancerdiagnosisprognosis.org)
In addition to this, pharmacological inhibition of the PI3K pathway in cancer cells leads to up-regulation of receptor tyrosine kinases through feedback loops that circumvent baseline level of PI3K inactivation. (cancerdiagnosisprognosis.org)
Studies have shown that harpagide may inhibit Aβ25-35 induced apoptosis and oxidative stress of PC12 cells through activation of Phosphatidylinositol 3-Kinase (PI3K)/Protein Kinase B (Akt) signaling pathway, indicating its potential value in the treatment of Alzheimer's disease[ 7 ]. (ijpsonline.com)
In a recent study under review at the journal Molecular Medicine and currently available on the Research Square * preprint server, researchers in China investigate the role of phosphatidylinositol 3 kinase (PI3K)/ protein kinase B (AKT) pathway in causing metabolic disruptions and tissue injury among human corneal epithelial cells (hCECs) and corneal tissues of mice. (worldmedicinefoundation.com)
Western blot analysis demonstrated a significant reduction in the p-AKT/AKT ratio in the two-day group, with significantly lowered phosphorylated ribosomal protein S6 kinase beta-1 (p-p70S6K)/p70S6K ratios in the one-day and two-day groups, thus indicative of PI3K/AKT/p70S6K signalling inhibition by exposure to blue light. (worldmedicinefoundation.com)
Cyclin A2 and cyclin-dependent kinase 2 (CDK 2) levels, both of which are PI3K/AKT pathway substrates, were significantly reduced among the zero-hour LY294002, two-day DMSO, and two-day LY294002 treatment groups as compared to the zero-hour DMSO group. (worldmedicinefoundation.com)
The most well-known mechanism of endocrine resistance is cross-talk between the estrogen receptor (ER) and other growth factor signaling, such as phosphatidylinositol-3-kinase (PI3K)/Akt and the mammalian target of rapamycin (mTOR) signaling pathway. (ewha.ac.kr)
Western blot analysis was used to quantify expression of proteins involved in apoptosis, cell cycle, and phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) signaling. (bvsalud.org)
In addition, MEG3-shRNA activated the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) pathway, which was reversed by miR-181b inhibitor. (engreen.com.cn)
The current work aimed to examine whether inflammatory cytokines regulate mechanical allodynia and thermal hyperalgesia induced by diabetes through the phosphoinositide 3-kinase (PI3K)-mammalian target of rapamycin (mTOR) pathway in the PAG. (epain.org)
Immunoblot and ELISA were performed to examine PAG protein amounts of interleukin-1β (IL-1β), IL-6, and tumor necrosis factor-α (TNF-α), as well as their corresponding receptors in STZ rats, and the expression of PI3K/protein kinase B (Akt)/mTOR signaling effectors. (epain.org)
Additionally, as a downstream event of the phosphatidylinositol 3-kinase (PI3K)-mTOR signaling pathway [ 6 ], the regulation of pain sensitization by the PI3K-mTOR pathway through a central mechanism warrants further assessment. (epain.org)

Signals from receptor tyrosine kinases can promote proliferation through the MAP kinase pathway (left branch) and survival through the PI3 kinase pathway (right branch). (nih.gov)
c) Green tea catechins inhibit VEGF-induced IL-8 activation, and that this inhibition is mediated through a decrease in the PI3-kinase/Akt pathway leading to decrease of NF-kB activation. (nih.gov)
This hypothesis will be tested by blocking the PI3-kinase/Akt pathway using wortmanin inhibition and measuring IL-8 production, IL-8 mRNA expression, and NF-kB activation. (nih.gov)
Transcriptional regulation of human mucin MUC4 by bile acids in oesophageal cancer cells is promoter-dependent and involves activation of the phosphatidylinositol 3-kinase signalling pathway. (nih.gov)
On the basis of immunoprecipitation experiments, in vitro kinase assay and RNA interference, we demonstrate that VEGF-A induces the c-ABL kinase activity through the VEGF Receptor-2 /Phosphatidylinositol-3-Kinase pathway. (unisi.it)
Here, using different experimental approaches, especially designer receptor exclusively activated by designer drug (DREADD) chemogenetic technology, we found that sustained activation of G q -protein signaling impairs the functionality of striatal neurons and we unveil the precise molecular mechanism underlying this process: a phospholipase C/Ca 2+ /proline-rich tyrosine kinase 2/cJun N -terminal kinase pathway. (jneurosci.org)
As an example, Pim-1 serine/threonine kinase (PIM1) regulates cell proliferation and survival that might lead to reduction of therapeutic drug efficacy by promoting the Janus kinases/signal transducer and activator of transcription proteins (JAK/STAT) signaling pathway in MYC proto-oncogene (MYC)-driven cancer ( Nat Rev Cancer 11(1) (2011)">13 - Mol Cancer Ther 9(9) (2010)">16 ), particularly in breast cancer. (cancerdiagnosisprognosis.org)
Fibroblast Growth Factor Receptor, Human Epidermal growth factor Receptor, Phosphatidylinositol 3-kinase/AKT/mTOR pathway), angiogenesis (e.g. (endourology.ph)
The phosphatidylinositol 3-kinase (PI3-kinase)-Akt-mTOR pathway is mutated at high frequency in human breast cancer, and this pathway is the focus of active drug discovery and clinical investigation. (oncotarget.com)
These findings validate novel driver mutations in AKT1 , and extend the number and type of mutations that activate the PI3-kinase pathway in human breast cancers. (oncotarget.com)
In at least some tumor cells, sigma antagonists cause calcium-dependent activation of phospholipase C and concomitant calcium-independent inhibition of phosphatidylinositol 3'-kinase pathway signaling. (hw.ac.uk)

Non-receptor tyrosine kinases regulate cell growth, proliferation, differentiation, adhesion, migration and apoptosis, and they are critical components in the regulation of the immune system. (wikipedia.org)
The cell proliferation was analyzed using the cell counting kit-8 assay. (cancerindex.org)
Cell-cycle distribution, proliferation, and apoptosis were detected by CCK-8 assay and flow cytometry. (biomedcentral.com)
The MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay and cyclin D1 protein expression analysis revealed that NAP treatment significantly reduces viability and proliferation of the cells. (uniroma5.it)
2010. (-)-Epigallocatechin-3-gallate inhibits Met signaling, proliferation, and invasiveness in human colon cancer cells. . (oregonstate.edu)
Background/Aim: Tyrosine kinases have crucial functions in cell signaling and proliferation. (cancerdiagnosisprognosis.org)
The effect of lycopene-based agents on prostate cancer growth and survival were examined using proliferation assays, bromodeoxyuridine incorporation and flow cytometric analysis of cellular DNA content. (nih.gov)
RESULTS@#The results of CCK-8 assay showed that berberine significantly inhibited the proliferation of RA-FLSs in a time- and concentration-dependent manner. (bvsalud.org)

Ultraviolet-induced phosphorylation of p70(S6k) at Thr(389) and Thr(421)/Ser(424) involves hydrogen peroxide and mammalian target of rapamycin but not Akt and atypical protein kinase c. (cdc.gov)
The p70 S6 kinase (p70(S6k)) is a Ser/Thr kinase that plays an important role in cell growth, transformation, and the transition of the cell cycle in mammalian cells. (cdc.gov)
These results demonstrated that H(2)O(2), phosphatidylinositol 3-kinase, and mammalian target of rapamycin were important players for UV-induced p70(S6k) phosphorylation at Thr(389) and Thr(421)/Ser(424), whereas Akt and atypical protein kinase C were not involved in this activation. (cdc.gov)
PIKfyve, first described in 1999, 1 is a mammalian protein and lipid kinase that controls complex and distinct cellular functions (reviewed by Shisheva et al . (haematologica.org)

9. The effect of inhibitors of phosphatidylinositol 3-kinase-related kinases on dibenzo[def,p]chrysene genotoxicity measured by γH2AX levels and neutral comet assay in HepG2 human hepatocellular cancer cells. (nih.gov)
We have established a protocol for the use of this novel probe in fixed cell preparations and have verified its specificity for PIP3 by using binding competitors, such as inositol-(1,3,4,5)-tetrakisphosphate, and inhibitors of PIP3 generation, such as the specific inhibitor of PI3-kinase activity LY294002. (lancs.ac.uk)
The discovery of oncogenic alterations with sensitivity to tyrosine kinase inhibitors (TKIs) has changed the therapeutic landscape of lung cancer. (e-crt.org)
By using specific pharmacological inhibitors of mitogen-activated protein kinase, phosphatidylinositol 3-kinase, protein kinase A and protein kinase C, we demonstrate that bile acid-mediated up-regulation of MUC4 is promoter-specific and mainly involves activation of phosphatidylinositol 3-kinase. (nih.gov)
Tyrosine kinase inhibitors (genistein or herbimycin), Src family inhibitor (PP2), or a phosphatidylinositol-phospholipase C inhibitor (U73122) attenuated the IFN-γ-induced ICAM-1 expression. (wustl.edu)
Protein kinase C (PKC) inhibitors (staurosporine or Ro 31-8220) also inhibited IFN-γ-induced response. (wustl.edu)
The IFN-γ-induced ICAM-1 promoter activity was inhibited by tyrosine kinase inhibitors, a phosphatidylinositol-phospholipase C inhibitor, or PKC inhibitors, and the TPA-induced ICAM-1 promoter activity was also inhibited by tyrosine kinase inhibitors. (wustl.edu)
The epidermal growth factor receptor ( EGFR ) and phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha ( PIK3CA ) single gene assays in plasma cell free DNA are being used for selecting patients with metastatic lung and breast cancer for treatment with EGFR and PIK3CA inhibitors, respectively. (bjmo.be)
Materials and Methods: To mimic catalytic inactivation of p110α/β, specific p110α (BYL719) and p110β (KIN193) inhibitors were used in addition to genetic knock-out in in vitro assays. (cancerdiagnosisprognosis.org)
It is indicated for adults with Philadelphia chromosome-positive chronic myeloid leukemia (Ph+ CML) in chronic phase (CP) who were previously treated with ≥2 tyrosine kinase inhibitors (TKIs). (medscape.com)

By treating HUVEC with the specific tyrosine kinase inhibitor STI571 and over-expressing a dominant negative c-ABL mutant, we show that the VEGF-A-activated c-ABL reduces the amplitude of Mitogen-Activated Protein Kinases (ERK1/2, JNKs and p38) activation in a dose-dependent manner by a negative feedback mechanism. (unisi.it)
The most well-characterized protein kinases in the human genome are protein serine/threonine kinases and protein tyrosine kinases ( Methods Enzymol 200 (1991)">2 ). (cancerdiagnosisprognosis.org)

We aimed to study the molecular mechanisms of how the gastric pathogen Helicobacter pylori hijacks cortactin phosphorylation via tyrosine kinase Abl to trigger cancer-related signal transduction events. (mdpi.com)

Vascular endothelial cell dysfunction is initial process of atherosclerosis[ 3 ] and inhibition of vascular endothelial cell injury is helpful for atherosclerosis therapy. (ijpsonline.com)
Inhibition of the PtdIns(5) kinase PIKfyve disrupts intracellular replication of Salmonella. (atilravillaallende.com.ar)

A non-receptor tyrosine kinase (nRTK) is a cytosolic enzyme that is responsible for catalysing the transfer of a phosphate group from a nucleoside triphosphate donor, such as ATP, to tyrosine residues in proteins. (wikipedia.org)
Non-receptor tyrosine kinases are a subgroup of protein family tyrosine kinases, enzymes that can transfer the phosphate group from ATP to a tyrosine residue of a protein (phosphorylation). (wikipedia.org)
Unlike the receptor tyrosine kinases (RTKs), the second subgroup of tyrosine kinases, the non-receptor tyrosine kinases are cytosolic enzymes. (wikipedia.org)
Thirty-two non-receptor tyrosine kinases have been identified in human cells (EC 2.7.10.2). (wikipedia.org)
Unlike receptor tyrosine kinases, nRTKs lack receptor-like features such as an extracellular ligand-binding domain and a transmembrane-spanning region. (wikipedia.org)
Non-receptor tyrosine kinases do not contain only a tyrosine kinase domain, nRTKs also possess domains that mediate protein-protein, protein-lipid, and protein-DNA interactions. (wikipedia.org)
VEGF signaling is a multicomponent complex comprising vascular endothelial (VE)-cadherin/ b-catenin,/VEGF-receptor 2 (Flk-1/KDR)/ phosphatidylinositol 3'(PI3)-kinase. (nih.gov)
We propose to examine this model by testing the hypothesis: a) Green tea catechins disrupt VE-cadherin/ VEGF-receptor/ PI3-kinase complex formation. (nih.gov)
IQGAP1 binds the Axl receptor kinase and inhibits its signaling. (nih.gov)
Epidermal growth factor receptor ( EGFR ), anaplastic lymphoma kinase ( ALK ), and ROS proto-oncogene 1 ( ROS1 ) are 'must-test' biomarkers in the molecular diagnostics of advanced-stage lung cancer patients. (e-crt.org)
In particular, epidermal growth factor receptor ( EGFR ) mutations, anaplastic lymphoma kinase ( ALK ) rearrangements, and ROS proto-oncogene 1 ( ROS1 ) rearrangements have been frequently used as therapeutic targets, and they are regarded as "must-test" biomarkers in the molecular diagnostics of advanced-stage lung cancer patients [ 1 ]. (e-crt.org)
2009. Tea catechins inhibit hepatocyte growth factor receptor (MET kinase) activity in human colon cancer cells: kinetic and molecular docking studies. . (oregonstate.edu)
Zeta chain of T-cell receptor associated protein kinase 70 (ZAP70) is a cytoplasmic tyrosine phosphoprotein. (cancerdiagnosisprognosis.org)
ZAP70 has a crucial role in T-cell receptor signaling activation and T-cell development ( Immunity 5(3) (1996)">18 ). (cancerdiagnosisprognosis.org)
Erdafitinib, an orally available pan-FGFR inhibitor, received accelerated approval based on impressive overall response rate and acceptable toxicity profile noted in a phase II trial in patients with metastatic bladder cancer harboring Fibroblast Growth Factor Receptor 2/3 genomic alterations noted on a companion diagnostic assay (RT-PCR by Qiagen). (endourology.ph)
In a receptor-binding assay, butylparaben was able to compete with 3H-estradiol for binding to the rat estrogen receptor with an affinity approximately 5 orders of magnitude lower than that of diethylstilboestrol, and between 1 and 2 orders of magnitude less than nonylphenol. (researchgate.net)

this effect was inhibited by tyrosine kinase or Src inhibitor. (wustl.edu)
BMS-345541 (a specific inhibitor of IκB kinase (IKK)) or wortmannin (a specific inhibitor of phosphatidylinositol 3-kinase (PI-3K)) inhibited the LPS-induced degradation of the ECM and apoptosis in chondrocytes. (biomedcentral.com)
THP-1 monocytes were pre-incubated with cinnamon extract, cinnamon fractions or its compounds and stimulated with lipopolysaccharides (LPS), followed by determination of interleukin 8 (IL-8) secretion, and phosphorylation of protein kinase B (Akt), nuclear factor (NF)-κB inhibitor alpha (IκBα) and p38. (rsc.org)
ALIQOPA is a kinase inhibitor indicated for the treatment of adult patients with relapsed follicular lymphoma (FL) who have received at least two prior systemic therapies ( 1 ). (nih.gov)
Asciminib is a tyrosine kinase inhibitor that binds to ABL myristoyl pockets, thereby inhibiting ABL1 kinase activity of the BCR-ABL1 fusion protein. (medscape.com)

Phosphoinositide kinases regulate diverse cellular functions and are important targets for therapeutic development for diseases, such as diabetes and cancer. (nih.gov)
Enzymatic assays for phosphoinositide kinases often use lipid substrates prepared from lyophilized lipid preparations by sonication, which result in variability in the liposome size from preparation to preparation. (nih.gov)

Preparation of the lipid substrate is crucial for the development of a robust and miniaturizable lipid kinase assay. (nih.gov)
2 ). PIKfyve phosphorylation of protein substrates includes autophosphorylation 3 while lipid kinase activity generates two phosphorylated species of phosphatidylinositol (PtdIns):-5-P and -3,5-P2. (haematologica.org)

Hyperglycemia induced the activation of mitogen-activated protein kinase/extracellular signal-regulated protein kinase and/or phosphatidylinositol-3 kinase/Akt pathways in a time-dependent manner. (uniroma5.it)
These compensatory mechanisms include forkhead box O (FOXO) transcription factors along with mitogen-activated protein kinase (MAPK)-dependent pathways ( Cancer Discov 9(4) (2019)">11 , Cancer Cell 21(2) (2012)">12 ). (cancerdiagnosisprognosis.org)
IκBα, the serine/threonine kinase Akt and mitogen-activated protein (MAP) kinase p38, who can activate the transcription factors NF-κB and/or activator protein 1 (AP-1). (rsc.org)

Furthermore, BMS-345541 and/or wortmannin suppressed the LPS-induced upregulation of catabolic enzymes that mediate ECM degradation (matrix metalloproteinases-9, -13), cyclooxygenase-2 and apoptosis (activated caspase-3). (biomedcentral.com)
EC-7072 directly triggered caspase-3-dependent CLL cell apoptosis, which was not abrogated by microenvironment-derived factors that sustain leukemia cell survival. (frontiersin.org)

Although single-gene assays are currently considered the gold standard for these genes, next-generation sequencing (NGS) tests are being introduced to clinical practices. (e-crt.org)
Patients with lung cancer who received both conventional single-gene assays and subsequent targeted NGS testing were enrolled, and the results of their tests were compared. (e-crt.org)
The discordant cases were mostly false-negatives of the single-gene assays, probably due to technical limitation. (e-crt.org)
Currently, single-gene assays, such as real-time polymerase chain reaction (PCR), immunohistochemistry (IHC), and fluorescence in situ hybridization (FISH) tests, are considered the gold standard for selecting eligible patients for EGFR -, ALK -, and ROS1 -specific TKI therapy [ 1 , 2 ]. (e-crt.org)
In this study, we aimed to compare the results of single-gene assays and NGS testing for EGFR , ALK , and ROS1 and assess the occurrence of false results associated with these methods in the molecular diagnostics of lung cancer patients. (e-crt.org)

We newly elucidated that miR-143 targeted akt and that miR-145 targeted integrin-linked kinase (ilk) in T24 cells based on the results of a luciferase activity assay. (nih.gov)
The transcriptional activity of β-catenin was measured using luciferase activity assay. (ewha.ac.kr)

500 in vitro assays in phase I (complete) and Phase II (under way). (nih.gov)

Interferon-γ (IFN-γ) induced intercellular adhesion molecule-1 (ICAM-1) expression in human NCI-H292 epithelial cells, as shown by enzyme-linked immunosorbent assay and immunofluorescence staining. (wustl.edu)
Inflammasome activation and pyroptosis of VK2/E6E7 cells were analyzed using microscopy, Hoechst 33342/propidium iodide staining, lactate dehydrogenase release assay, gene and protein expression, co-immunoprecipitation, immunofluorescence, and enzyme-linked immunosorbent assay. (jcimjournal.com)
Enzyme-linked immunosorbent assay kits were used to detect malondialdehyde content as well as superoxide dismutase and glutathione peroxidase activities. (ijpsonline.com)
Enzyme-linked immunosorbent assay (ELISA) was applied to evaluate the levels of pro-inﬂammatory cytokines. (engreen.com.cn)

b) Green tea catechins inhibit VEGF-induced PI3-kinase activation. (nih.gov)
7. SC-III3, a novel scopoletin derivative, induces cytotoxicity in hepatocellular cancer cells through oxidative DNA damage and ataxia telangiectasia-mutated nuclear protein kinase activation. (nih.gov)
In order to confirm PI3-kinase activation it is necessary to prove that the levels of its main product PIP3 rise in vivo. (lancs.ac.uk)
In agreement with results obtained from in vitro PI3- kinase and PKB activity assays, Bcr/Abl activation also induces increased PIP3 generation in the absence of IL-3. (lancs.ac.uk)
Interestingly, it was found that Src, Syk, IRAK1, and IRAK4 responsible for NF- κ B and AP-1 activation were identified as the direct molecular targets of KF by kinase enzyme assays and by measuring their phosphorylation patterns. (hindawi.com)
Thus, in NCI-H292 epithelial cells, IFN-γ activates PLC-γ2 via an upstream tyrosine kinase to induce activation of PKC-α and c-Src or Lyn, resulting in activation of STAT1α, and GAS in the ICAM-1 promoter, followed by initiation of ICAM-1 expression and monocyte adhesion. (wustl.edu)
The induction of NF-κB correlated with activation of IκBα kinase, IκBα phosphorylation, IκBα degradation, p65 phosphorylation and p65 nuclear translocation. (biomedcentral.com)
This resistance was via activation of the Janus kinase/signal transducer and activator of transcription 3 (JAK/STAT3) axis. (cancerdiagnosisprognosis.org)

Finally, we describe the main assays that can be used to analyze the complete sequence of autophagosome formation and degradation or autophagy flux, mainly in cultured microglia and in vivo. (bvsalud.org)
Furthermore, we established an assay measuring autophagy as a predictive marker of APY0201 sensitivity. (haematologica.org)
PtdIns-3,5-P2 is involved mainly in intracellular trafficking and lysosomal acidification and is thus crucial for the regulation of macroautophagy (herein denominated autophagy). (haematologica.org)

Design of a Targeted Sequencing Assay to Detect Rare Mutations in Circulating Tumor DNA. (cdc.gov)

The assays measured direct interactions between chemicals and molecular targets (receptors, enzymes), as well as downstream effects on reporter gene activity or cellular consequences. (nih.gov)

Binding relationships among HOTAIR, miR-20b-5p, and RRM2 were confirmed using dual-luciferase assay. (biomedcentral.com)
The interaction between lncRNA MEG3 and microRNA (miR)-181b were confirmed by Starbase and dual-luciferase reporter assay. (engreen.com.cn)

The Z'-factor value was greater than 0.7 for the PI5P4Kα assay, indicating its suitability for high-throughput screening applications. (nih.gov)

Quantitative reverse-transcription polymerase chain reaction (qRT-PCR), Western blot (WB), cell scratch assay, and cell reactive oxygen species (ROS) assays were performed. (worldmedicinefoundation.com)

High levels of tyrosine phosphorylation correspond to enhanced proliferative state ( N Engl J Med 353(2) (2005)">3 ). (cancerdiagnosisprognosis.org)
Lycopene exposure can suppress phosphatidylinositol 3-kinase-dependent proliferative and survival signalling in androgen-responsive LNCaP and androgen-independent PC3 cells suggesting that the molecular mechanisms for the cytostatic and cytotoxic actions of lycopene involve induction of G0/G1 cell cycle arrest. (nih.gov)

Endurance exercise results in DNA damage as detected by the comet assay. (oregonstate.edu)

2002. Sequencing of the rat beta-catenin gene (Ctnnb1) and mutational analysis of liver tumors induced by 2-amino-3-methylimidazo[4,5-f]quinoline. . (oregonstate.edu)
BACKGROUND This study was designed to illustrate the effects and latent mechanism of lncRNA maternally expressed gene 3 (MEG3) on intracerebral hemorrhage (ICH)-induced brain injury. (engreen.com.cn)
The heterogeneous behavior of CLL is, at least in part, underpinned by the presence of cytogenetic aberrations and the degree of somatic hypermutation in the immunoglobulin heavy-chain variable gene (IGHV), which bear great prognostic and therapeutic value ( 3 ). (frontiersin.org)

Loss of infectivity correlated with 70% reduced fusogenic activity of virions in liposome fusion assays. (uni-koeln.de)
Enables protein kinase B binding activity. (nih.gov)
Phosphatidylinositol-(3,4,5)-trisphosphate (PIP3) is a phospholipid second messenger generated by the activity of phosphatidylinositol 3-kinase in response to many hematopoietic growth factors. (lancs.ac.uk)
These results thus present the exciting hypothesis that hematopoietic growth factors and Bcr/Abl can stimulate nuclear PI3-kinase activity to generate concentrated localized increases in this important second messenger. (lancs.ac.uk)
Cell counting kit-8 assay and viral load analysis were used to evaluate the antiviral activity of JZ-1. (jcimjournal.com)
Finally, the same three mutants showed oncogenic activity in a cellular transformation assay. (oncotarget.com)

The migrative and invasive abilities of T24 cells were measured by the transwell assay. (cancerindex.org)

One of the important domain of nRTKs is the tyrosine kinase catalytic domain, which is about 275 residues in length. (wikipedia.org)

We tested this hypothesis using several strategies, including binding assays with pure proteins, cultured cells and knockout mouse models. (nih.gov)

Cell viability was assessed using crystal violet staining, whereas cellular transformation ability was analyzed by soft-agar growth assays. (cancerdiagnosisprognosis.org)
CK-8 assay findings showed significantly lower hCECs viability in the two-day group as compared to the zero hour group. (worldmedicinefoundation.com)

Immunoblot screening indicated that lycopene's antiproliferative effects are likely achieved through a block in G1/S transition mediated by decreased levels of cyclins D1 and E and cyclin dependent kinase 4 and suppressed Retinoblastoma phosphorylation. (nih.gov)

Stimulation of normal hematopoietic cells with Interleukin-3 (IL-3) produces a consistent increase in the total amount of cellular fluorescence compared with unstimulated cells. (lancs.ac.uk)

Likewise, deletion of chromosome 17 del(17p) or chromosome 11 del(11q) are associated with poor therapeutic response and short survival ( 3 ). (frontiersin.org)

Experimental and molecular pathology 2016 Apr 100 (3): 421-425. (cdc.gov)

At the molecular level, various cytokines (e.g., tumor necrosis factor- (TNF-) α ), hydrolytic enzymes, toxic molecules (e.g., nitric oxide (NO) and reactive oxygen species (ROS)), and mediators (e.g., prostaglandin E 2 (PGE 2 )) are released from inflammatory cells [ 2 , 3 ]. (hindawi.com)
UV radiation also generated reactive oxygen species as measured by electron spin resonance and by H(2)O(2) and O( minus sign, dot below )(2) fluorescence staining assays in JB6 Cl 41 cells. (cdc.gov)

Using our in vitro tube formation assay by the human microvascular endothelial cells (HMVEC), we have tested the inhibitory effect of catechins on vascular endothelial growth factor (VEGF} signaling during angiogenesis. (nih.gov)
Analysis of fixed cells stained with the GFPprobe by confocal microscopy confirms that IL-3 and Bcr/Abl both stimulate an increase in PIP3 levels. (lancs.ac.uk)
Electrophoretic gel mobility shift assay demonstrated that GAS-binding complexes in IFN-γ-stimulated cells contained STAT1α. (wustl.edu)
The phytochemical 3,3'-diindolylmethane decreases expression of AR-controlled DNA damage repair genes through repressive chromatin modifications and is associated with DNA damage in prostate cancer cells. (oregonstate.edu)
Withhold treatment until ANC ≥0.5 x 10 3 cells/mm 3 ( 5.5 ). (nih.gov)

Development of SH2 probes and pull-down assays to detect pathogen-induced, site-specific tyrosine phosphorylation of the TLR adaptor SCIMP. (atilravillaallende.com.ar)

NPJ precision oncology 2019 11 3 28. (cdc.gov)

Cancer detection sensitivity from 50-70% (3). (norgenbiotek.com)

NCI will conduct mouse lymphoma assay. (nih.gov)

One hour (h) or 3 days after ICH stimulation, rat neurological injury was evaluated by modified Neurological Severity Score (mNSS). (engreen.com.cn)

We will use a battery of cell biological assays , as well as multiphoton intravital imaging to dissect the mechanisms underlying cigarette smoke-evoked metastasis. (evomet-itn.eu)
The duration of hCEC exposure to blue light was assessed using the cell counting kit-8 (CCK-8) assays. (worldmedicinefoundation.com)
Cell , 135 (3): 561-71. (guidetopharmacology.org)

Anatomy19

Organisms8

Diseases1

Chemicals and Drugs174

Analytical, Diagnostic and Therapeutic Techniques and Equipment12

Phenomena and Processes37

Information Science3

PI3K23

Pathway11

Proliferation8

Mammalian4

Inhibitors10

Protein Kinases2

Signal Transduction1

Inhibition2

Receptor16

Inhibitor5

Phosphoinositide kinases2

Lipid kinase2

Mitogen-Activat3

Apoptosis2

Single-gene assays5

Luciferase activity assay2

Vitro assays1

Enzyme-linke4

Activation8

Autophagy3

Mutations1

Downstream1

Dual-luciferase2

High-throughput1

Scratch assay1

Proliferative2

Comet assay1

Gene3

Activity6

Transwell1

Catalytic1

Proteins1

Viability2

Cyclin1

Cellular1

Survival1

20161

Reactive2

Cells5

Tyrosine phosphorylation1

20191

Sensitivity1

Lymphoma1

Stimulation1

Cell3