• 2 , 3 CDK6 together with CDK4 are serine/threonine kinases that positively regulate the G1 phase progression in association with D-type cyclin. (ashpublications.org)
  • BACKGROUND: : Inherited mutations in the CDKN2A tumor suppressor gene, which encodes the p16(INK4a) protein, and in the cyclin-dependent kinase 4 (CDK4) gene confer susceptibility to cutaneous malignant melanoma. (lu.se)
  • We analyzed families with two or more cases of melanoma for germline mutations in CDKN2A and CDK4 to elucidate the contribution of these gene defects to familial malignant melanoma and to the occurrence of other cancer types. (lu.se)
  • METHODS: : The entire CDKN2A coding region and exon 2 of the CDK4 gene of an affected member of each of 52 families from southern Sweden with at least two cases of melanoma in first- or second-degree relatives were screened for mutations by use of polymerase chain reaction-single-strand conformation polymorphism analysis. (lu.se)
  • The 113insArg mutant p16(INK4a) was unable to bind cdk4 and cdk6 in an in vitro binding assay. (lu.se)
  • Based on a database search, we identified abemaciclib, an FDA-approved Cdk4/Cdk6 inhibitor used for the treatment of metastatic breast cancer, as potent inhibitor of HIPK2, HIPK3, and DYRK1A. (nature.com)
  • Remarkably, DYRK1A is inhibited by abemaciclib to the same extent as Cdk4/Cdk6 in vitro, raising the question of whether targeting of DYRK1A contributes to the transcriptional inhibition and therapeutic activity of abemaciclib. (nature.com)
  • It has been shown to form complex with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF-1, MOK, as well as eIF2 alpha kinases. (nih.gov)
  • The protein encoded by this gene is a member of the CMGC family of serine/threonine protein kinases. (cancerindex.org)
  • What does this gene/protein do? (cancerindex.org)
  • What pathways are this gene/protein implicaed in? (cancerindex.org)
  • Serum amyloid A-like 1 (also known as SAAL1, Synoviocyte proliferation-associated in collagen-induced arthritis 1, and SPACIA1) is a protein in humans encoded by the SAAL1 gene. (wikipedia.org)
  • This gene encodes a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. (cancerindex.org)
  • Cyclin/Cdk repression inhibits retinoblastoma protein (pRb) phosphorylation, thereby limiting the transcription of genes that directly promote the mechanics of the cell cycle, and we found that Pax6 inhibits pRb phosphorylation and represses genes involved in DNA replication. (ox.ac.uk)
  • Low expression of CDK6 protein in ZR-75-1, Neuro-2a, and INS-1 cells is consistent with the predicted expression pattern. (cellsignal.com)
  • Immunoprecipitation of CDK6 protein from Jurkat cell extracts. (cellsignal.com)
  • Current projects include compounds blocking HIV and other antivirals, glutamate transporter ( EAAT2 ) stimulators, ACSS2 Inhibitors, Proteolysis Targeting Chimeras ( PROTAC s) with a focus on CDK6 , Mdm2, and Tert degraders, small molecules targeting protein-protein interactions, chemical probes for assay development, and cancer targeting and gram-negative bacteria targeting pro-drugs. (wistar.org)
  • from Int J Paediatr Dent 2000 Dec;10(4):328-34) The X-linked form is also known as Zinsser-Cole-Engman syndrome and involves the gene which encodes a highly conserved protein called dyskerin. (lookformedical.com)
  • CDK6 is a protein known to be involved in cancer and has a drug to help treat this disease. (nih.gov)
  • The protein encoded by this gene is highly similar to Cdc 37, a cell division cycle control protein of Sacchromyces cerevisiae. (nih.gov)
  • Medium-Throughput Detection of Hsp90/Cdc37 Protein-Protein Interaction Inhibitors Using a Split Renilla Luciferase-Based Assay. (nih.gov)
  • The GSE3467 , GSE3678 , GSE33630 and GSE53157 were obtained from the GEO database, including 100 thyroid tumors and 64 normal tissues to obtain the intersection of differentially expressed genes, and a protein-protein interaction network was constructed to obtain the HUB gene. (peerj.com)
  • In addition, we have examined the putative role of HSP70-2 in apoptosis, cell cycle arrest and epithelial-mesenchymal transition (EMT) in-vitro and in-vivo xenograft mouse model using gene silencing approach. (biomedcentral.com)
  • Medicilon Biology Department has rich experience in the field of in vitro biology, and offers a complete set of biological services including enzyme level measurement, cell level measurement, cell biology, biochemistry, isotope measurement in vitro, establishment of stable cell lines, gene knockout, RNAi and MicroRNA technology. (medicilon.com)
  • Stability assays reveal that SAAL1 promotes G1/S transition via CDK6 mRNA stabilization. (wikipedia.org)
  • Histoculture drug response assay (HDRA) was used to examine docetaxel sensitivity, while qRT-PCR was used to measure the mRNA levels of the genes in 25 surgically dissected gastric cancer specimens. (iiarjournals.org)
  • Mutations in the genes BRCA1, BRCA2 or PIK3CA have important implications in the cellular DNA repair mechanisms, and are associated with sensitivity and improved response to platinum-based chemotherapy and PAPR inhibitors. (unilabs.ch)
  • Ingenuity pathway analysis was performed to integrate elevated kinase activity and gene over-expression with coexisting missense mutations at DNA level. (oncotarget.com)
  • Potentially relevant missense mutations were seen in 176 kinase genes, with the top altered pathways overlapping with gene overexpression being GNRH, NF-kB and STAT3 signaling. (oncotarget.com)
  • It is predicted that MCPH gene mutations may lead to the disease phenotype due to a disturbed mitotic spindle orientation, premature chromosomal condensation, signalling response as a result of damaged DNA, microtubule dynamics, transcriptional control or a few other hidden centrosomal mechanisms that can regulate the number of neurons produced by neuronal precursor cells. (biomedcentral.com)
  • Further, we review the corresponding genes and the proteins encoded by these genes, their possible role in the developing brain and reported mutations of these genes. (biomedcentral.com)
  • MCPH is inherited in an autosomal recessive pattern in which both copies of the gene in each cell have mutations. (biomedcentral.com)
  • DUSP4 alterations lead to hyperactivation of MAPK signaling in many cancers, including breast cancer, which often harbor mutations in cell cycle checkpoint genes, particularly in TP53. (biomedcentral.com)
  • Consistently, inactivating mutations ENOblock (AP-III-a4) in various NER genes cause the autosomal recessive syndrome xeroderma pigmentosum (XP), which is Nos1 usually associated with UV sensitivity and susceptibility to skin cancer development (4). (colinsbraincancer.com)
  • We also reported that inactivating mutations in or of any among other DDR genes mixed up in mobile response to replicative tension cripples NER exclusively in S stage. (colinsbraincancer.com)
  • The 3′UTR-binding sequence site of miR-128-5p on the p21 gene was predicted and assessed by luciferase assays. (hindawi.com)
  • RNA pull down assay, luciferase reporter assay and fluorescence in situ hybridization were applied to verify the interaction between BCRC-3 and microRNAs. (biomedcentral.com)
  • A) Luciferase reporter genes were determined in ZR75-1 and MDA-MB-231 breast cancer cells co-transfected with Glutaminase-IN-1 different concentrations of reporter and myc-ZKSCAN5. (bms-911543.com)
  • B) Luciferase reporter gene detection in ZR75-1 and MDA-MB-231 breast cancer cells co-transfected with expression in ZR75-1 and MDA-MB-231 cells, which were transfected with ZKSCAN5 shRNA, control shRNA, or ZKSCAN5 shRNA plus shRNA-resistant ZKSCAN5 (ZKSCAN5-R). The representative Western blot further showed the expression of ZKSCAN5. (bms-911543.com)
  • Based on all these data, we propose that miR-29a-3p, miR-34b-3p, miR-181c-5p and miR-517a-3p are disallowed tumor suppressor genes in neuroblastoma and suggest them as new therapeutic targets in neuroblastoma. (oncotarget.com)
  • Biochemical assays indicated that Pax6 directly represses Cdk6 expression. (ox.ac.uk)
  • See also {614327} for a tumor predisposition syndrome that may contribute to the development of malignant mesothelioma upon asbestos exposure and is caused by germline mutation in the BAP1 gene ( OMIM ) on chromosome 3p21. (mendelian.co)
  • Materials and Methods: Microarray analysis was performed to explore various gene expression levels between parental and docetaxel-resistant cells. (iiarjournals.org)
  • A panel of 11 genes selected according to microarray analysis were validated and tested further in 11 cancer cell lines, resulting in 4 genes, CXCR4, CDK6, USP15 and CDH1. (iiarjournals.org)
  • With the development of RNA sequencing and microarray technology, the study of differentially expressed genes (DEGs) between cancer and normal tissues has improved our understanding of the molecular mechanism of PTC. (peerj.com)
  • We compared the gene expression profiles of equivalent Pax6-expressing progenitors isolated from Pax6⁺/⁺ and Pax6⁻/⁻ cortices and identified many negatively regulated cell-cycle genes, including Cyclins and Cdks. (ox.ac.uk)
  • miR-128-5p directly targeted the 3′UTR-binding sequences of the p21 gene, negatively regulated p21 expression, and supported the proliferation of VSMCs. (hindawi.com)
  • The CDKN2A/B locus contains genes encoding cell cycle inhibitors, including p16 Ink4a , which have not yet been implicated in the control of hepatic glucose homeostasis. (diabetesjournals.org)
  • The t(7;14) involved the immunoglobulin heavy chain ( IgH ) locus in 14q32 and a transposon-like element located 29 kbp upstream of the CDK6 coding sequence. (ashpublications.org)
  • miR-503 inhibited significantly the expression of CDK6 and the downstream genes of E2F3. (biomedcentral.com)
  • We investigated a panel of 34 known high/moderate-risk cancer genes, including 16 related to breast or ovarian cancer (BC/OC) genes, and 63 candidate genes to BC/OC in 192 clinically suspicious of hereditary breast/ovarian cancer (HBOC) Spanish families without pathogenic variants in BRCA1 or BRCA2 (BRCA1/2). (cancerindex.org)
  • Also, expression verification and survival analysis of these candidate genes based on the TCGA database indicate the robustness of the above results. (peerj.com)
  • We observed a downregulation in the expression of genes involved in host response, such as TNF signaling, IL-1β production, and phagosome formation in Mφ, and phosphatidylinositol signaling and TNF signaling in PMNs, being in line with the ability of the pathogen to survive within phagocytes. (frontiersin.org)
  • The IDG program has developed molecular probes (small molecules), molecular tools and assays, transgenic mice (specialized genetic lines of laboratory mice), recombinant cell lines, data, and digital resources for 100+ understudied GPCRs, ion channels and kinases. (nih.gov)
  • We determined the crystal structures of HIPK3 and DYRK1A bound to abemaciclib, showing a similar binding mode to the hinge region of the kinase as observed for Cdk6. (nature.com)
  • Selective inhibition of Ph-positive ALL cell growth through kinase-dependent and -independent effects by CDK6-specific PROTACs. (wistar.org)
  • Next Generation Sequencing (NGS) of 517 kinase genes was performed using Agilent Kinome capture and run on the Illumina MiSeq at PE150bp. (oncotarget.com)
  • The NanoString nCounter® platform analyzed the expression of 519 kinase genes. (oncotarget.com)
  • Top pathways upregulated in both the kinase activity and gene expression platforms were PTEN, STAT3, GNRH, IL-8 and B cell receptor signaling. (oncotarget.com)
  • The multigene Onco type DX assay can predict who is going to benefit from chemotherapy and who is likely going to have a recurrence. (jhoponline.com)
  • 1 Chromosomal translocation leading to overexpression of the CDK6 gene, which is located in 7q21, has recently been described in splenic marginal zone lymphoma (SMZL). (ashpublications.org)
  • Overexpression of CDK6 is considered to contribute to leukemogenesis through dysregulation of cell proliferation, thereby favoring progression through the G1 phase. (ashpublications.org)
  • It seems likely that CDK6 overexpression is implicated in lymphomas with low proliferative index and, as we show here, may also be involved in other low-grade malignancies such as B-cell chronic lymphocytic leukemia (B-CLL). (ashpublications.org)
  • Marked overexpression of CDK6 was observed in all 3 patients with CDK6 rearrangement compared with normal peripheral blood lymphocytes and with B-CLL without CDK6 involvement ( Figure 1 ). (ashpublications.org)
  • As previously reported, 2 CDK6 overexpression can result from aberrant variable-diversity-joining (VDJ) or variable-joining (VJ) recombination, leading to the juxtaposition of the CDK6 gene with the Ig gene enhancer during B-cell differentiation. (ashpublications.org)
  • Overexpression of CDK6. (ashpublications.org)
  • Two unique NER subpathways have been recognized: global genomic NER (GG-NER) and transcription-coupled NER (TC-NER), which excise UV DNA photoproducts throughout the entire genome and exclusively from your ENOblock (AP-III-a4) transcribed strands of active genes, respectively. (colinsbraincancer.com)
  • The signature mechanism was studied by analyzing the gene ontology and the Kyoto Encyclopedia of Genes and Genome database. (peerj.com)
  • In 3 patients, involvement of the CDK6 gene was found by at least 2 different methods. (ashpublications.org)
  • We found that Dusp4 loss alone is insufficient in mediating tumorigenesis, but alternatively converges with loss in Trp53 and MYC amplification to induce tumorigenesis primarily through chromosome 5 amplification, which specifically upregulates Dbf4 , a cell cycle gene that promotes cellular replication by mediating cell cycle checkpoint escape. (biomedcentral.com)
  • miR-128-5p targets the tissue inhibitor of metalloproteinases (TIMPs), silent information regulator 2 (SIRT2), peroxisome proliferator-activated receptor (PPAR), and p21 genes, which are linked to the behaviours of VSMCs. (hindawi.com)
  • The obtained samples were washed with phosphate-buffered saline, cut into CDK6 Inhibitor Accession defined pieces (0.25 cm2) and transferred into a 50 mL centrifuge tube containing a pre-prepared remedy of 1 mL 2.5 trypsin and 25 mL PBS. (caspaseinhibitor.com)
  • MiaPaCa-2 and CFPAC-1 cells were treated with 100 ng/ml of NGF or the NGF inhibitor Tanezumab for 24 h, CCK-8 and Transwell assays were employed to test cell proliferation, invasion, and migration, respectively. (hindawi.com)
  • Here, we show that p16 Ink4a deficiency enhances fasting-induced hepatic glucose production in vivo by increasing the expression of key gluconeogenic genes. (diabetesjournals.org)
  • At CST, we adhere to the Hallmarks of Antibody Validation™ , six complementary strategies for determining the specificity, sensitivity, and functionality of an antibody in any given assay. (cellsignal.com)
  • In summary, our data indicate that PRGF caused considerable induction of a number of genes in primary human fibroblasts that happen to be critical for ECM formation. (caspaseinhibitor.com)
  • Unlike the amount of methylation of their encoding gene promoters, all these miRNAs were significantly overexpressed following treatment with 5'-AZA. (oncotarget.com)
  • We identified 24 common genes that were significantly altered in both PMNs and PBMCs, including genes involved in IFN signaling that were downregulated after treatment in both cell populations, and IL1R1 that was upregulated. (frontiersin.org)
  • Interestingly, most of their encoding genes reside in hypermethylated genomic regions: thus, their tumor suppressor function is normally disallowed in these tumors. (oncotarget.com)
  • 4 However, no material was available for CDK6 expression analysis in this case. (ashpublications.org)
  • FISH and Southern blot analysis showed that an interesting common feature was that in all 3 cases the CDK6 gene was rearranged by reciprocal translocations involving either Ig κ, Ig λ, or IgH locus and not with only the Ig κ light chain locus as has previously been reported in other series. (ashpublications.org)
  • This indicates that FISH and CDK6 expression analysis can be successfully used to identify patients with CDK6 involvement. (ashpublications.org)
  • Western blot analysis of extracts from various cell lines using CDK6 (E3E3Q) Rabbit mAb (upper) or GAPDH (D16H11) XP ® Rabbit mAb #5174 (lower). (cellsignal.com)
  • Western blot analysis was performed using CDK6 (E3E3Q) Rabbit mAb. (cellsignal.com)
  • Immunohistochemical analysis of paraffin-embedded normal human colon (two fields of view) using CDK6 (E3E3Q) Rabbit mAb (left) or a CDK6 Rabbit mAb (right). (cellsignal.com)
  • In this research, we conducted a multi-step analysis through comprehensive bioinformatics analysis to identify hub genes in THCA. (peerj.com)
  • Cell growth assay, cell cycle analysis, FACS, JC-1 staining, annexin V staining and immunoblotting were used to study the efficacy of ST on cancer cells. (biomedcentral.com)
  • Our Core Cancer panels consist of essential gene sets that provide necessary information for genetic tumor biomarker profiling at the molecular level. (unilabs.ch)
  • Pax6 exerts regional control of cortical progenitor proliferation via direct repression of Cdk6 and hypophosphorylation of pRb. (ox.ac.uk)
  • Our research showed that the migration, phenotypic transformation, and proliferation of ox-LDL-induced VSMCs were repressed by AA through inhibiting miR-128-5p by targeting the p21 gene, which may provide an effective option for the treatment of atherosclerosis. (hindawi.com)
  • A, B) Cell proliferation and colony formation assays in HLECs cultured in conditioned medium come from ZR75-1 or MDA-MB-231 cells stably infected with lentivirus carrying ZKSCAN5 shRNA or ZKSCAN5 shRNA plus ZKSCAN5-R. The representative Western blot displays the expression of ZKSCAN5. (bms-911543.com)
  • The aim of this study was to explore predictive genes involved in docetaxel sensitivity of gastric cancer. (iiarjournals.org)
  • We had analyzed circRNA high-throughout sequencing from human tissues and determined bladder cancer related circRNA-3 (BCRC-3, GenBank: KU921434.1) as a new candidate circRNA derived from PSMD1 gene. (biomedcentral.com)
  • This assay looks at 16 breast cancer-related genes and 5 reference genes, and places patients in 1 of 3 categories related to adjuvant chemotherapy based on a disease recurrence score ranging from 0 to 100. (jhoponline.com)
  • Altered expression of this gene has been observed in multiple human cancers. (cancerindex.org)
  • The human SAAL1 gene is located at position 11p15.1 on the minus strand spanning from base pairs 18080292-18106082 (25,790 bases). (wikipedia.org)
  • These two antibodies detect unique, non-overlapping epitopes on human CDK6. (cellsignal.com)
  • In addition, the potential for these genes to perform various cognitive roles during human brain evolutionary processes is discussed. (biomedcentral.com)
  • A scratch wound-healing assay was used to detect the migration of VSMCs, and immunocytochemistry and western blotting for SM22ɑ were used to evaluate phenotypic transformation. (hindawi.com)
  • This was accompanied by elevated migration in a scratch assay. (caspaseinhibitor.com)
  • Gsk2118436 is a medication for the treatment of cancers associated with a mutated version of the gene BRAF. (gsk2118436inhibitor.com)
  • After cloning the antibody genes into an expression vector, this is then transfected into an appropriate host cell line for antibody expression. (cellsignal.com)
  • The in vivo hematopoietic reconstitution potential of c-kit + cells derived from the mouse PSCs was evaluated via m-NSG transplantation assay. (biomedcentral.com)
  • Studies of the rock bream SAAL1 ortholog noted an increase in gene expression in response to bacterial and viral pathogens. (wikipedia.org)
  • This assay is designed to provide a thorough profile of the tumor and its behavior which is relevant for treatment response in standard of care but also emerging investigational clinical actions. (unilabs.ch)
  • p 0.01 versus the control shRNA group (A, B). (C, D) Wound healing assays for HLECs cultured in conditioned medium from ZR75-1 or MDA-MB-231 cells, which were Glutaminase-IN-1 stably infected as in (A). The image shown is one of the representative results (C, D). Scale bar: 100 m. (bms-911543.com)