Assays division cell cycle. Medical search. Frequent questions

The aim of this study was to define algorithms for predicting positive TB cultures using antibody-based assays of TMKmyc levels in vitro . (biomedcentral.com)
Results: LED produced in vitro increases of cell growth of 140-200% in mouse-derived fibroblasts, rat-derived osteoblasts, and rat-derived skeletal muscle cells, and increases in growth of 155-171% of normal human epithelial cells. (researchgate.net)
In vitro studies using infra-/red light have not shown any useful impact on wound healing by activating cells located in deeper skin layers such as fibroblasts or stem cells [2] . (researchgate.net)
Concentrations were tested up to 8333 μg enzyme concentrate dry matter/mL), a recommended regulatory maximum concentration for the in vitro micronucleus assay. (europa.eu)
The aim of this paper is to analyse sunitinib malate in vitro ability to enhance cisplatin cytotoxicity in T24, 5637, and HT1376 human urinary bladder-cancer cell lines. (hindawi.com)
This investigation aims to analyse the in vitro effects of cisplatin and sunitinib malate in isolation and in combination, on one human nonmuscle invasive urinary bladder-cancer cell line (5637) and on two human muscle-invasive urinary bladder-cancer cell lines (T24 and HT1376). (hindawi.com)
Measured are only the cells in vitro and/or in vivo that are actively dividing. (baseclick.eu)
In vitro neurosphere assays revealed striking increases in the number of neurosphere forming cells from p107 −/− brains that exhibited enhanced capacity for self-renewal. (rupress.org)
Downregulation of PI3K-C2β delays mitosis progression in PC3 and HeLa cells, resulting in reduced ability to form colonies in clonogenic assays in vitro. (biomedcentral.com)
Compared to control cells, PC3 cells lacking PI3K-C2β form smaller and more compact colonies in vitro and they form tumours more slowly in vivo in the first weeks after cells implant. (biomedcentral.com)
Combination of PI3K-C2β downregulation and docetaxel almost completely prevents colonies formation in clonogenic assays in vitro and strongly inhibits tumour growth in vivo. (biomedcentral.com)
In vitro dynamics assays show that, like mosaic brain microtubules, recombinant homogenous microtubules undergo dynamic instability but they polymerize slower and catastrophe less frequently. (bbk.ac.uk)
This hypothesis is also supported by in vitro data showing an antiproliferative activity of BN80927 on a model of resistance related to the noncycling state of cells (G 0 -G 1 synchronized). (aacrjournals.org)
These cells can be isolated from different tissues in the adult human body, expanded in vitro and applied for the regeneration of tissues and organs damaged by age, disease or extensive injury. (mdpi.com)
In vitro studies also revealed that GFAT1 promoted the proliferation, cell cycle progression, migration and invasion of HCC cells. (oncotarget.com)
Rat primary astrocytes were isolated from different brain regions (cortex, cerebellum, and hippocampus) and grown in vitro to demonstrate the utilization of live-cell analysis for monitoring and comparing the diversity of cultured astroglia phenotypes. (news-medical.net)

Using EdU shows several advantages over other cell proliferation assays (BrdU or 3H-thymidine) making it a superior evaluation tool for monitoring genotoxicity, evaluation of anticancer drugs or cell cycle analysis. (baseclick.eu)

This gene is thought to regulate cell cycle progression. (wikipedia.org)
In different assays and cell types, overexpression of this protein has been shown to activate or suppress cell cycle progression. (wikipedia.org)
It further inhibited cell-cycle progression in the G1 phase by four different mechanisms: rapid downregulation of cyclin D1, induction of Chk2 with simultaneous downregulation of Cdc25A, induction of the Cdk-inhibitor p21 Cip/Waf and inhibition of ribonucleotide reductase activity resulting in reduced dCTP and dTTP levels. (nature.com)
Methyl jasmonate represses growth and affects cell cycle progression in cultured Taxus cells. (chemspider.com)
The persistent fork progression in HU-treated Cdk2AF/AF cells indicates that Cdk2 inhibitory phosphorylation is required for normal Corynoxeine execution of the S-phase checkpoint induced by replication stress. (cancerrealitycheck.com)
In cell cycle progression studies cells were Corynoxeine incubated for 48-52 h in serum- and leucine-free media (MP Biomedicals) and released into media containing 40 ng/mL nocodazole to prevent entry into the next cell cycle. (cancerrealitycheck.com)
Alteration of signalling pathways regulating cell cycle progression is a common feature of cancer cells. (biomedcentral.com)
MT dynamics) playing a key role in the normal progression of this cycle to insure proper cell division (Fig. 1). (cytoskeleton.com)

Both the immunofluorescence assay and electron microscopy showed that TbCen2 and 3-deficient cells were enlarged in size with duplicated basal bodies, multinuclei and new flagella that are detached along the length of the cell body. (elsevierpure.com)
This biopsy material tested positively for La Crosse virus antigen on direct immunofluorescence assay. (medscape.com)

It is induced by p53 in response to DNA damage, or by sublytic levels of complement system proteins that result in activation of the cell cycle. (wikipedia.org)
The protein also translocates to the nucleus in response to treatment with complement system proteins, and can associate with and increase the kinase activity of cell division cycle 2 protein. (wikipedia.org)
The expression of pro-apoptotic proteins and anti-apoptotic proteins in MCF-7 and MDA-MB-231 cells was investigated by Western blotting. (researchsquare.com)
Egg extracts, in which the cell cycle state can be manipulated, contain stockpiles of nuclear proteins (including condensin and cohesin) sufficient for the assembly of thousands of nuclei per microliter. (nih.gov)
For cohesion assays, demembranated sperm nuclei are incubated in interphase extract, where they undergo rapid and synchronous DNA replication and cohesion establishment through the recruitment of proteins and other factors (e.g., nucleotides) from the extract. (nih.gov)
Centrins are calcium binding proteins involved in cell division in eukaryotes. (elsevierpure.com)
Cells are programmed to express fluorescent proteins at various points in the cell cycle, allowing the researchers to link cell cycle information to growth. (mit.edu)
Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (6). (cellsignal.com)
Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA (reviewed in 7). (cellsignal.com)
Such dependant enzymes include those of the citric acid cycle, the mitochondrial electron transport chain, as well as several other pathways in the metabolism of carbohydrates, fats, and proteins. (medscape.com)

How does EdU labeling compare to BrdU or the 3H-thymidine incorporation assay? (baseclick.eu)

MyeloCult™ media have been developed for the long-term culture of hematopoietic progenitor cells in association with adherent stromal cells. (stemcell.com)
Adult p107-null mice had elevated numbers of proliferating progenitor cells in their lateral ventricles. (rupress.org)
The first Special Issue "Mesenchymal Stem/Progenitor Cells and Their Derivates in Tissue Regeneration" has been recognized by many scientific groups and was a successful endeavor. (mdpi.com)
Mesenchymal stem/progenitor cells (MSCs) reside in many human tissues and organs and play important roles in tissue regeneration and tissue homeostasis. (mdpi.com)

Cdk2AF cells also exhibited strikingly abnormal responses to replication stress accumulated irreparable DNA damage and permanently exited the cell cycle after transient exposure to S-phase inhibitors. (cancerrealitycheck.com)
We conclude that Cdk2 inhibitory phosphorylation is required for normal replication dynamics in asynchronous and arrested cells. (cancerrealitycheck.com)
Cells that possess a pyrimidine pathway that can phosphorylate EdU to the corresponding triphosphate, which is then accepted by the host DNA polymerase for incorporation into DNA during replication. (baseclick.eu)
UTR shortening of an essential regulator of DNA replication, CDC6 (cell division cycle 6), upon E2 treatment. (omicsdi.org)
Therefore, if any of these cell lines or eggs support productive replication of SARS-CoV-2, then a validated procedure should be implemented to rule out the presence of SARS-CoV-2 in the specimens before their inoculation. (cdc.gov)
We conducted this study to determine whether cell lines and eggs commonly used to isolate and propagate influenza viruses, poliovirus, and other human viruses can support productive replication of SARS-CoV-2. (cdc.gov)

This study is the first to show that VALD-3 induces apoptosis and cell cycle arrest in human breast cancer cells by suppressing Wnt/β-catenin signaling, indicating that it could be a potential drug for the treatment of breast cancer. (researchsquare.com)

More recently, newer molecular assays for TB have emerged based on serology (detecting the 38 kDa antigen, Early Secretory Antigen or ESAT-6, CFP10 and other secretory antigens), nucleic acid amplification (NAATS), phage amplification and line probe assays for MDR [ 10 ]. (biomedcentral.com)

We examined hematopoietic stem cells at four stages of differentiation, lineage-committed progenitors, and mature myeloid and lymphoid cells. (nih.gov)
The telomerase-expressing somatic cells observed in this study are not thought to be immortal, and expression was not correlated with cell cycle distribution or differentiation state. (nih.gov)
This behavior is crucial for cell division, motility and differentiation. (bbk.ac.uk)
Cells bearing MSC characteristics differ in the facets of proliferation and differentiation, which results in their diverse biological potential and applications in tissue regeneration. (mdpi.com)
The ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in cell differentiation, cell division, cell adhesion, and stress responses. (enzolifesciences.com)

Cell cycle adaptations of embryonic stem cells. (nih.gov)
Exendin-4 promotes proliferation of adipose-derived stem cells through ERK and JNK signaling pathways. (chemspider.com)
Among bone marrow hematopoietic stem cells, 70% exhibited detectable telomerase activity. (nih.gov)
It is also responsible for inhibition of vascular endothelial growth factors receptors, plateletderived growth factor receptors, and stem cell factor receptor [ 8 , 9 ]. (hindawi.com)
Here we show a novel function for Retinoblastoma family member, p107 in controlling stem cell expansion in the mammalian brain. (rupress.org)
This encouraged us to continue this Special Issue with a second part devoted to research concerning mesenchymal stem cell application in regenerative medicine. (mdpi.com)

So far, the Emeryville, California-based firm has built its business by providing customers, usually pharmaceutical companies interested in antibody production, with the ability to trap and assay cells using proprietary, optics-based technology developed by Ming Wu, a professor at the University of California, Berkeley. (genomeweb.com)
Immunohistochemical analysis of paraffin embedded Jurkat cell pellets, untreated (left) or etoposide-treated (right), using Phospho-Histone H3 (Ser10) Antibody, PCNA (PC10) Mouse mAb, Cleaved Caspase-3 (Asp175) Antibody and Survivin (71G4B7E) Rabbit mAb. (cellsignal.com)
Each antibody is validated for use in immunohistochemical assays using multiple approaches. (cellsignal.com)

Endometrium-derived epithelial cells (Ishikawa) showed no response to the natural compound by using a cell viability assay (MTT). (nutrimedical.com)
8 Astrocytes interact with blood vessels, pericytes, and epithelial cells to organize CNS flow and modulate the blood-brain barrier (BBB). (news-medical.net)
Immortalized human lung epithelial cells, BEAS-2B, were exposed to all MW for 24 hours. (cdc.gov)
However, this preliminary evaluation indicates that human lung epithelial cells exposed to AP-MW and their coated counterparts produces a genotoxic response. (cdc.gov)

Protein activation and expression were analysed by western blotting, deoxyribonucleoside triphosphate levels by HPLC, ribonucleotide reductase activity by 14 C-cytidine incorporation into nascent DNA and cell-cycle distribution by FACS. (nature.com)
MTT colorimetric assays, colony formation assays and 5‑ethynyl‑20‑deoxyuridine incorporation assays were also conducted to evaluate ACC cell proliferation. (cancerindex.org)
After incorporation of EdU, cell proliferation can be detected in different organs. (baseclick.eu)
All three methods enable to determine cell proliferation directly by incorporation of a metabolite analogue and subsequent detection. (baseclick.eu)

This gene, which encodes a member of the serine/threonine kinase family, regulates cell polarity and functions as a tumor suppressor. (cancerindex.org)
1) LTS and STS of de novo resected PDAC show clear differences in cancer cell protein activation re-key markers of proliferation and apoptosis (Sox2, Ki67, STAT3, SRC kinase, H3S10). (thevirusproject.org)

Regulator of cell cycle RGCC (RGCC) also known as response gene to complement 32 protein (RGC-32) is a protein that in humans is encoded by the RGCC gene. (wikipedia.org)
Effects of triterpenes from Ganoderma lucidum on protein expression profile of HeLa cells. (springer.com)
Chromosome structure in both interphase and M-phase cells is strongly influenced by the action of the cohesin and condensin protein complexes. (nih.gov)
For apoptotic inducing activity, human osteogenic sarcoma (HOS) cell proliferation, chromosomal DNA degradation and changes in apoptosis-related protein levels were measured. (nutrimedical.com)
Preclinical data reported here show that BN80927 retains Topo I poisoning activity in cell-free assay (DNA relaxation) as well as in living cells, in which in vivo complexes of topoisomerase experiments and quantification of DNA-protein-complexes stabilization, have confirmed the higher potency of BN80927 as compared with the Topo I inhibitor SN38. (aacrjournals.org)
2) For stromal cells, protein activation was largely similar, but a difference in activation of one stromal protein (Mek S298) was seen. (thevirusproject.org)
The Beacon platform can trap up to 56,000 cells in so-called "light pens" and then assay them to provide data on cell shape, size, protein output, or other important features. (genomeweb.com)
Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy (3). (cellsignal.com)
Moreover, a D614G mutation in the spike protein did not appear to affect SARS-CoV-2 cell tropism. (cdc.gov)
Entry of the vitamin into cells is mainly facilitated by carrier-mediated transport through a specific riboflavin-binding protein on cell membranes. (medscape.com)
DeMarini, and Chapter 20, by Rice and cell death determine the size protein in several signal ing path- and Herceg). (who.int)
Nineteen national-level public health laboratories performed routine dengue diagnostic assays on a proficiency testing panel consisting of two modules: one containing commercial serum samples spiked with cultured dengue viruses for the detection of nucleic acid and non-structural protein 1 (NS1) (Module A) and one containing human serum samples for the detection of anti-dengue virus antibodies (Module B). A review of logistics arrangements was also conducted. (who.int)

After treatment with β-iminophosphonate (E,Z)(3-Methyl-2-(pyridin2-ylimino)butyl)diphenylphosphine oxide (4e) proliferation/viability of cancer cells was strongly inhibited and in 65.61% of the cells signs of early apoptosis were observed. (japsonline.com)

Specific immunoglobulin M (IgM) and neutralizing antibodies to the virus in serum or cerebrospinal fluid (CSF) using enzyme-linked immunosorbent assay (ELISA) technique: IgM is usually but not always present during acute illness. (medscape.com)
a nutri- ied cases by enzyme-linked immunosorbent tional rehabilitation intervention programme assay (ELISA) according to Chauveau et al. (who.int)

In this assay hematopoietic cells are cultured in MyeloCult™ medium with supportive human or mouse stromal cell layers and tested for their ability to generate myeloid clonogenic progenitors for several weeks as assayed by replating the cells in a colony-forming unit (CFU) assay using MethoCult™ medium. (stemcell.com)
Clonogenic assays were performed to determine the ability of PC3 and HeLa cells to form colonies upon PI3K-C2β downregulation in the absence or presence of docetaxel. (biomedcentral.com)

Khz, a naturally occurring compound derived from the fusion of Ganoderma lucidum and Polyporus umbellatus mycelia, inhibits the growth of cancer cells. (springer.com)
Survivin binds and inhibits caspase-3, controlling the checkpoint in the G2/M-phase of the cell cycle through inhibiting apoptosis and promoting cell division (4,5). (cellsignal.com)

Silencing oncogene cell division cycle associated 5 induces apoptosis and G1 phase arrest of non-small cell lung cancer cells via p53-p21 signaling pathway. (nih.gov)

Detection of S-Phase of Cell Division Cycle in Plant Cells and Tissues by Using 5-Ethynyl-2? (chemspider.com)
Assays based on high-affinity detection of secretory TMKmyc levels in culture using specific antibodies are considered. (biomedcentral.com)
Incorporating "chemiluminiscent or fluorescent" strategies may enable "photo-detection of TMKmyc changes" and hence automation of the entire assay. (biomedcentral.com)
Can I perform EdU cell proliferation detection on living cells? (baseclick.eu)
EdU is incorporated into living cells, but the detection reaction must be performed on fixed and permeabilized samples. (baseclick.eu)

These data show that di-GA exhibits three distinct anticancer activities: induction of apoptosis, cell-cycle arrest and disruption of cancer cell-induced lymphendothelial disintegration. (nature.com)
Flow cytometry analysis showed that VALD-3 triggered cell cycle arrest and induced apoptosis of breast cancer cells. (researchsquare.com)
Moreover we found that Cdk2AF/AF cells replicated more DNA during HU arrest than Cdk2+/+ cells as shown by the increased track lengths in HU-treated Cdk2AF/AF cells compared with untreated cells (Fig. 5 and for details on statistical analysis). (cancerrealitycheck.com)
For S-phase arrest cells were treated with HU or APH for 16-18 h and released into media containing 40 ng/mL nocodazole. (cancerrealitycheck.com)
A cell cycle arrest in early S-phase and in G 0 /G 1 -phase was also found after exposure to cisplatin and sunitinib malate, in isolation, respectively. (hindawi.com)

Khz cytotoxicity was measured using an MTT assay and the mitochondrial membrane potential (MMP)-related, calcium-induced generation of reactive oxygen species (ROS) in A549 cells was measured by flow cytometry. (springer.com)
Flow cytometry analysis showed that the percentage of A549 cells in sub-G1 phase of the cell cycle increased in response to Khz treatment. (springer.com)
3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, acridine orange, and monodansylcadaverine staining and flow cytometry were performed. (hindawi.com)
They contain special reagents and a dedicated protocol for read-outs using flow cytometry on cells in suspension. (baseclick.eu)
To avoid this problem, the researchers developed a way to precisely control the flow in the system so that a cell could be stopped anywhere in the bypass channel. (mit.edu)

Together with dynactin, dynein regulates centrosomal orientation to establish and maintain cell polarity, controls focal adhesion turnover and anchors microtubules at the leading edge. (biologists.com)
In addition, these drugs are known to induce serious side effects due to inhibition of mitosis in other proliferating cells (mainly bone marrow and gut) as well as inhibition of other microtubules-dependent functions, such as neuronal processes [ 18 , 19 ]. (biomedcentral.com)

Findings answer puzzling question of how cells know when to progress through the cell cycle. (mit.edu)
Our findings should help avert inadvertent propagation of SARS-CoV-2 from diagnostic cell lines. (cdc.gov)

The antitumor activity and survival analysis of VALD-3 in vivo was determined by the nude mice xenograft assay. (researchsquare.com)
Tumour growth in vivo was assessed using a xenograft model of PC3 cells upon PI3K-C2β downregulation and in combination with docetaxel. (biomedcentral.com)

Thus, the secretion of gall wasps stimulates plant cell growth and overrules homeostasis of the affected leaf area - this is similar to tumour outgrowth. (nature.com)
In turn, the plant produces GA, which seems to combat the improper growth signals and re-establishes cell-cycle control. (nature.com)
Accordingly, pre-treatment with 350 nM LSD1 inhibitor for 3 days followed by exposure to serial dilutions of ruxolitinib led to synergistic growth inhibition in non-DS-AMKL and ML-DS cell lines (Supplementary Fig. 4 ), as well as in all ML-DS patient samples (Fig. 1A ). (nature.com)
By xenografting human breast cancer cells into nude mice, we found that VALD-3 significantly suppressed tumor cell growth while showing low toxicity against major organs. (researchsquare.com)
Effects of Ganoderma lucidum spores on HepG2 cells proliferation and growth cycle. (springer.com)
Since DNA duplication during interphase precedes microbial division, "para-DNA synthesis assays" could be used to predict impending microbial growth. (biomedcentral.com)
In the INH resistance scenario, it is shown that despite the presence of optimal doses of INH in LJ to stop M. tb proliferation, bacilli grow and the resulting phenotypic growth changes in colonies/units are predictable through the TMKmyc assay. (biomedcentral.com)
Cell Cycle Analysis Growth Assays and Micronucleation. (cancerrealitycheck.com)
For growth assays cells were seeded on day 0 at 2 0 cells per well in a 96-well plate. (cancerrealitycheck.com)
The class II PI3K isoform PI3K-C2β was downregulated in prostate cancer PC3 cells and cervical cancer HeLa cells using selective siRNAs and the effect on cell growth was determined in the absence or presence of the microtubule-stabilizing agent/anti-cancer drug docetaxel. (biomedcentral.com)
Stable and transient PI3K-C2β downregulation potentiates the effect of low concentrations of docetaxel on cancer cell growth. (biomedcentral.com)
Furthermore, data indicate that blockade of PI3K-C2β might represent a novel strategy to potentiate the effect of docetaxel on cancer cell growth. (biomedcentral.com)
In cell growth assays, BN80927 is a very potent antiproliferative agent as shown by IC 50 values consistently lower than those of SN38 in tumor cell lines as well as in their related drug-resistant lines. (aacrjournals.org)
The abnormal segregations ultimately led to aborted cytokinesis and hence affected growth in these cells. (elsevierpure.com)
Triticum aestivum L. root growth inhibition test and Allium cepa L. assay were used to assess the cytotoxic potential of phosphonates. (japsonline.com)
Bulk cell-washing of Prorocentrum PA1, followed by growth with antibiotics, was only effective in reducing bacterial load in the initial growth stages, but did not yield axenic cultures or lower bacterial cell densities throughout the culture cycle. (awi.de)
There was no significant correlation between Prorocentrum cell volume, growth rate, bacterial cell counts, or cellular toxin concentration over the entire time-series culture cycle. (awi.de)
Benthic Prorocentrum and associated bacterial communities comprise highly diverse and characteristic microbiomes upon substrates, and among compartments in culture, but this study provides little evidence that allelochemical interactions among Prorocentrum cells and associated bacteria originating from epibenthic substrates play a definable role in growth and toxigenicity. (awi.de)
However, determining if this holds true for mammalian cells has been difficult, in part because there has been no good way to measure mammalian cell growth over time. (mit.edu)
Now, a team of MIT and Harvard Medical School (HMS) researchers has precisely measured the growth rates of single cells, allowing them to answer that fundamental question. (mit.edu)
In the Aug. 5 online edition of Nature Methods , the researchers report that mammalian cells divide not when they reach a critical size, but when their growth rate hits a specific threshold. (mit.edu)
In the new study, Manalis and his colleagues were able to track cell growth and relate it to the timing of cell division by measuring cells' mass every 60 seconds throughout their lifespans. (mit.edu)
It's easier for cells to measure their growth rate, because they can do that by measuring how fast something in the cell is produced or degraded, whereas measuring size precisely is hard for cells," Son says. (mit.edu)
A cell devotes itself to growth in a phase called G1. (mit.edu)
Once cells complete the transition into S phase, growth rates diverge again. (mit.edu)
This article discusses how a combination of live-cell analysis methods deliver the required flexibility to allow these highly dynamic astrocytic models to be characterized, via quantification of their growth, motility, morphology, and functional analysis. (news-medical.net)
The Incucyte ® Live-Cell Analysis System delivers flexible kinetic quantification of biological phenotypes to monitor growth, motility, morphology, and cellular function. (news-medical.net)
Temporal monitoring of brain region astroglia revealed differences in cell growth and morphology. (news-medical.net)

Induction of apoptosis by ethanol extracts of Ganoderma lucidum in human gastric carcinoma cells. (springer.com)
In contrast, because chromosome condensation occurs spontaneously in M-phase extracts, sperm nuclei are added directly to CSF extracts to assay condensation. (nih.gov)
No bacteriostatic or allelochemical response was found for cell- and culture medium extracts of five Prorocentrum isolates assayed for bioactivity against Staphylococcus sp. (awi.de)

Avoid freeze/thaw cycles. (enzolifesciences.com)

Avant Diagnostics, Inc. ('Avant') (OTC: AVDX), an oncology-focused healthcare technology company commercializing the proprietary Theralink proteomic biomarker platform announced positive clinical utility data, the confirmation of the clinical utility of the Theralink Pancreatic Cancer assay in an expanded 60 patient data study in distinguishing patients that will experience a 5-year survival outcome following standard of care treatment regimens, versus those likely to experience significantly worse outcomes. (thevirusproject.org)

The SignalStain ® Proliferation/Apoptosis IHC Sampler Kit from Cell Signaling Technology allows the researcher to examine paraffin-embedded tissues or cells with antibodies that will detect cellular apoptosis or proliferation. (cellsignal.com)

Using high throughput sequencing and computational approaches, I reconstruct the lineage of B cells that produce HIV-specific bnAbs, working backward, up to a pre-HIV infection timepoint. (wrfseattle.org)
albopictus are important vectors and Aedes japonicus also may be involved in virus maintenance and transmission (ref https://read.qxmd.com/doi/10.2987/moco-31-03-233-241.1) Alternating cycles of infection occur between the mosquito and the vertebrate hosts, including humans. (medscape.com)
Human immunodeficiency virus (HIV) infection results from 1 of 2 similar retroviruses (HIV-1 and HIV-2) that destroy CD4+ lymphocytes and impair cell-mediated immunity, increasing risk of certain infections and cancers. (msdmanuals.com)

Establish stromal cell layers and culture hematopoietic progenitors in "Dexter-type" long-term bone marrow cultures. (stemcell.com)
Stromal cell feeder layers (e.g. (stemcell.com)
Laser Capture Microdissection enabled directed cancer and stromal cell isolation. (thevirusproject.org)

The amount of EdU used in the assays shows no toxicity in cells as well as in animals thus does not influence the results of experiments. (baseclick.eu)

TMKmyc assays may be useful for reducing the time-lines to positive identification of Mycobacterium tuberculosis (M. tb ) cultures, thereby accelerating disease diagnosis and drug resistance profiling. (biomedcentral.com)
Isolating and propagating viruses from clinical specimens in cell cultures or embryonated chicken eggs is widely used to identify multiple viruses and produce vaccines, mostly under Biosafety Level 2 containment. (cdc.gov)

Ganoderic acid Mf and S induce mitochondria mediated apoptosis in human cervical carcinoma HeLa cells. (springer.com)
Cell lines such as E. coli, HeLa, HEK, MOLM are arguably among the most routine applications, but also animals, like mouse, rat, the nematode C. elegans, crickets (Gryllus bimaculatus), chicken (Gallus domesticus) and zebra fish (Danio rerio) or even plants (e.g. (baseclick.eu)
To answer this question, we first investigated the ability of ellagic acid to influence the activity of the estrogen receptor subtypes ERalpha and ERbeta in HeLa cells. (nutrimedical.com)

Downregulation of CDCA5 Can Inhibit Cell Proliferation, Migration, and Invasion, and Induce Apoptosis of Prostate Cancer Cells. (nih.gov)

The disruption of MT dynamics, and thereby the cell cycle, leads to cell death. (cytoskeleton.com)

The scope of the special issue is to summarize and enlarge the knowledge in signalling processes and networks in diverse cells and tissues. (mdpi.com)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)

Loss of cell division cycle‑associated 5 promotes cell apoptosis by activating DNA damage response in clear cell renal cell carcinoma. (nih.gov)

MTs serve as an essential component of a cell's cytoskeleton as they regulate and participate in a variety of cellular functions that include motility, morphology, intracellular transport, signal transduction, and cell division (Fig. 1). (cytoskeleton.com)

Preliminary two-color cell cycle analysis showed no evidence of cell cycle disruption after 24 hour treatment to 2.4 microg/cm2 MW. (cdc.gov)

As it is known that marked shifts in osmolality can give rise to chromosome aberrations (and thereby micronuclei), these observations cast some doubt over the biological significance of the observed increases in MNBN cells observed. (europa.eu)

Avant provides personalized medicine diagnostic testing capabilities through its TheraLink Diagnostic Assays, primarily for breast cancer, to assist clinical oncologists in identifying likely responders for over 30 FDA-approved drug treatment regimens. (thevirusproject.org)

It has been proposed that the biological clock underlying the limited division potential of eukaryotic cells is telomere length. (nih.gov)
The EdU cell proliferation assay has been applied to many different cell types and organisms from prokaryotic to eukaryotic. (baseclick.eu)

SOD, CAT and GPX activities were significantly increased in ellagic acid-treated V79-4 cells. (nutrimedical.com)
Ellagic acid significantly reduced HOS cell proliferation, and induced apoptosis evidenced by chromosomal DNA degradation and apoptotic body appearance. (nutrimedical.com)
The number, diversity, and complexity of astroglia cells have increased significantly with evolution, and research from several groups implies that gender differences exist. (news-medical.net)

Although we repeated all results under the same or slightly different conditions, some of our results were further confirmed using multiple assay methods on divergent SARS-CoV-2 strains and in cell lines from different sources. (cdc.gov)
[ 7 ] FAD is also a coenzyme needed for the functioning of the antioxidant enzyme glutathione reductase in its protection of cells against oxidative stresses, allowing for the measurement of the enzyme's activity in red blood cells to be among the methods for the assessment of riboflavin nutritional status. (medscape.com)

Specific areas of interest include understanding the mechanisms, which allow cancer cells to develop resistance to chemotherapy at the cellular and molecular levels. (sc.edu)

Senesence-associated (SA)-β-gal and micronucleation assays were performed as described (35 48 Adeno-Associated Virus (AAV) Gene Targeting. (cancerrealitycheck.com)
Cells co-transfected with an estrogen response element (ERE)-driven luciferase (Luc) reporter gene and an ERalpha- or ERbeta-expression vector were exposed to graded concentrations of ellagic acid. (nutrimedical.com)
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. (yeastgenome.org)

In this study, we investigated the cytotoxic and apoptotic effects of VALD-3, a Schiff base ligand synthesized from o-vanillin derivatives, on human breast cancer cells and the possible underlying mechanisms. (researchsquare.com)
Asymmetric cell divisions (ACDs) generate two daughter cells with identical genetic information but distinct cell fates through epigenetic mechanisms. (elifesciences.org)
Both extrinsic and intrinsic mechanisms determine distinct daughter cell fates after ACD. (elifesciences.org)
Caenorhabditis elegans represents a valuable model for investigating ACD, given its invariant cell lineage and conserved mechanisms of ACD. (elifesciences.org)

Present address: Department of Cell and Developmental Biology, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, 08003 Barcelona, Spain. (biologists.com)
1 Program in Cell Cycle and Cancer Biology, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma 73104. (nih.gov)
3 Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104. (nih.gov)
It's a longstanding question in biology: How do cells know when to progress through the cell cycle? (mit.edu)
The use of real-time, live-cell analysis provides new possibilities and cell biology workflows. (news-medical.net)

Genotoxicity was measured via micronuclei assay with pancentromere staining and two-color cell cycle analysis. (cdc.gov)

5 yr OS in such patients (pts), we analyzed proteomic activation (phosphorylation) patterns in both cancer and stromal cells from initial surgical specimens prior to adjuvant therapy (Rx). (thevirusproject.org)

VALD-3 also regulated the Wnt/β-catenin signaling pathway in breast cancer cells, inhibiting the activation of downstream molecules. (researchsquare.com)

J Cell Biol, 2007 Jan 1. (nih.gov)
Mol Cell Biol, 2006 Jul. (nih.gov)
J Cell Biol (2004) 166 (6): 853-863. (rupress.org)

Anticancer activity on human fibrosarcoma cell line HT 1080 was assessed. (japsonline.com)
Among tested compounds β-iminophosphonate '(E,Z)(3-Methyl-2-(pyridin-2-ylimino)butyl) diphenylphosphine oxide (4e) showed anticancer activity against human fibroblastoma cell line HT 1080. (japsonline.com)

Dr. Lim has more than 18 years of experience in cell sorting, cell cycle analysis, apoptosis and immunophenotyping. (sc.edu)
For cell cycle analysis cells were trypsinized and fixed in 70% (vol/vol) ethanol at 4 °C overnight. (cancerrealitycheck.com)
The assay can be used for single cell proliferation analysis as well as cell division in animals. (baseclick.eu)
Further analysis of the cells with defective organelle segregation identified three different sub configurations of organelle mis-segregations (Type 1-3). (elsevierpure.com)
Packed red cells are processed, stored, and shipped to the Division of Environmental Health Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention for analysis. (cdc.gov)
Using this data, it is possible to see the potential that live-cell analysis provides to the research of such dynamic and heterogeneous cell types. (news-medical.net)

In stromal cells, activation of the survival marker Mek S298 was lower (p=0.021). (thevirusproject.org)

Apoptosis was measured by Hoechst 33258/propidium iodide double staining of nuclear chromatin and the formation of gaps into the lymphendothelial barrier in a three-dimensional co-culture model consisting of MCF-7 tumour cell spheroids and human lymphendothelial monolayers. (nature.com)
and negative regulation of meiotic nuclear division. (nih.gov)
It was identified that the nuclear division cycle 80, cyclin B2 and topoisomerase 2‑α may serve important roles in adrenocortical tumor development. (cancerindex.org)
The antiproliferative effect of compounds was determined by WST-1 test, and nuclear morphology changes in cancer cells stained with Giemsa were evaluated. (japsonline.com)

Zhao S, Ye G, Fu G, Cheng JX, Yang BB, Peng C. Ganoderma lucidum exerts anti-tumor effects on ovarian cancer cells and enhances their sensitivity to cisplatin. (springer.com)

Low-risk types (e.g., types 6 and 11) can cause benign or low-grade cervical cell changes, genital warts, and recurrent respiratory papillomatosis ( 4 ). (cdc.gov)
High-risk types (e.g., types 16 and 18) can cause low-grade cervical cell abnormalities, high-grade cervical cell abnormalities that are precursors to cancer, and cancers ( 5 - 7 ). (cdc.gov)

Anatomy29

Organisms16

Diseases4

Chemicals and Drugs101

Analytical, Diagnostic and Therapeutic Techniques and Equipment23

Phenomena and Processes85

Disciplines and Occupations1

Information Science4

Health Care2

Vitro16

Proliferation Assays1

Progression8

Immunofluorescence assay2

Proteins10

BrdU1

Progenitor cells4

Replication6

Apoptosis and cell cycle arrest1

Antigen1

Differentiation5

Stem6

Antibody3

Epithelial cells4

Incorporation4

Kinase2

Protein12

Viability1

ELISA2

Clonogenic2

Inhibits2

Induces apoptosis1

Detection5

Arrest5

Flow5

Microtubules2

Findings2

Vivo2

Growth28

Extracts3

Freeze-thaw cycles1

Biomarker1

Antibodies1

Infection3

Stromal cell3

Toxicity1

Cultures2

HeLa3

Prostate cancer1

Dynamics1

Tissues2

Renal cell carc1

Signal Transduction1

Disruption1

Increases1

Diagnostic1

Eukaryotic2

Significantly3

Methods2

Cellular1

Gene3

Mechanisms4

Biology5

Genotoxicity1

Phosphorylation1

Signaling pathway1

Biol3

Anticancer2

Analysis6

Survival1

Nuclear4

Tumor1

Cervical2