• A viability assay is an assay that is created to determine the ability of organs, cells or tissues to maintain or recover a state of survival. (wikipedia.org)
  • Frogging" is a type of viability assay method that utilizes an agar plate for its environment and consists of plating serial dilutions by pinning them after they have been diluted in liquid. (wikipedia.org)
  • Their in vitro effects on hypoxic cells concerning cell viability, apoptosis, migration, and angiogenesis were detected through gain- and loss-of-function assays. (hindawi.com)
  • This in-vitro assay detected carcinogens and other toxicants that promoted tumors through epigenetic mechanisms. (cdc.gov)
  • For the in vitro toxicity assay, each substance was applied to mucosa fibroblast cell cultures in conditioned media. (bvsalud.org)
  • For the end-point of skin sensitization, the ultimate goal, i.e. the development and validation of alternative in vitro/in silico assays by 2013, may be achieved through a better understanding of the skin sensitization process on the cellular and molecular levels. (cdc.gov)
  • Thus, the principle of protein or peptide haptenation could be used in in vitro assays to predict the sensitization potential of a new chemical entity. (cdc.gov)
  • Once sensitized local lymph node assay (LLNA) (6), as no vali- to that chemical, the person is susceptible to dated in vitro alternatives exist to date (7). (cdc.gov)
  • It is likely that should lead to useful insights for the development improving our understanding of the cellular and of in vitro assays (17, 18). (cdc.gov)
  • Such inactivated reference materials were used as positive controls in assays to detect suspected B. anthracis in samples because live agent cannot be shipped for use in field settings, in improvement of currently deployed detection methods or development of new methods, or for quality assurance and training activities. (cdc.gov)
  • The bedrock of successful biodetection platforms and sensors is use of well-characterized molecular assays, immunoassays, or other types of detection assays. (cdc.gov)
  • The sensitive assay procedure involves a single addition of ATP detection cocktail directly to cells cultured in a serum-supplemented medium. (sigmaaldrich.com)
  • The ATP Cell Viability Luciferase Assay is a flash-type luminescence assay designed for individual sample detection by using a luminometer in a single sample format or a luminometer with an injector in 96-well plate format. (sigmaaldrich.com)
  • Linear detection range 0.12 µM (0.78 µg/dL) to 10 µM (65 µg/dL) zinc in 96-well assay format. (immpact-international.org)
  • LANSING, Mich., May 31, 2023 - Neogen® Corporation (NASDAQ: NEOG) announced today that it has launched two new assays for the detection of histamine, Reveal® for Histamine and Reveal Q+ for Histamine. (neogen.com)
  • Reveal and Reveal Q+ assays are easy-to-use lateral flow tests that utilize a simple water extraction method, providing results in a matter of minutes and eliminating the need to dispose of any chemicals, as is needed with other commercially available histamine detection methods, reducing testing time. (neogen.com)
  • ABSTRACT We evaluated the utility of 2 methods for detection of methicillin-resistant Staphylococcus aureus (MRSA) directly from signal-positive blood culture bottles: loop-mediated isothermal amplification (LAMP) assay, and direct cefoxitin disk diffusion (DCDD) test using a 30 g cefoxitin disk. (who.int)
  • Of 60 blood cultures positive for Gram-positive cocci in clusters, LAMP (via detection of the Fem A and Mec A genes) showed 100% sensitivity and specificity for identification of MRSA/MSSA. (who.int)
  • Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. (abcam.com)
  • ExoTEST is a patented double sandwich ELISA assay for quantitative and qualitative analysis of exosomes. (immpact-international.org)
  • ExoTEST consists of ELISA plates pre-coated with patented panexosome antibodies that allow specific capture of exosomes from a variety of biological samples, including cell culture supernatants and human biological fluids. (immpact-international.org)
  • The Antibody Hybridoma Core offers hybridoma, clone and subclone supernate screening by enzyme-linked immunosorbent assay (ELISA), Western blot assay and fluorescence-activated cell sorting (FACS). (mayo.edu)
  • Enzyme-linked immunosorbent assay (ELISA). (mayo.edu)
  • As with many kinds of viability assays, quantitative measures of physiological function do not indicate whether damage repair and recovery is possible. (wikipedia.org)
  • Reveal Q+ for Histamine (PN 9549) is a quantitative lateral flow assay designed to be read on Neogen's Raptor® Integrated Analysis Platform. (neogen.com)
  • The assay provides quantitative results for histamine in scombroid species of fish and dry animal protein. (neogen.com)
  • Expression of SIRT1/SOX9/LCN2 in the hypoxic cells was determined, and their interactions were predicted by bioinformatics websites and followed by the verification by luciferase assay and chromatin immunoprecipitation (ChIP). (hindawi.com)
  • What is more, dual-luciferase assay and western blotting were used to demonstrated the relationship between miRNA-149 and FOXC1. (hindawi.com)
  • ATP Cell Viability Luciferase Assay A highly sensitive firefly luciferase cell based assay for quantifying ATP in cell cultures used to measure cell viability. (sigmaaldrich.com)
  • The ATP Cell Viability Luciferase Assay offers a highly sensitive homogenous assay for quantifying ATP in cell cultures. (sigmaaldrich.com)
  • Store ATP Cell Viability Luciferase Assay at -80°C. Avoid repeated freeze-thaw cycles. (sigmaaldrich.com)
  • NEST has launched over 200 plastic consumable products for cell culture, molecular bislogy, immunoassays, liquid handling and storage. (dksh.com)
  • BioAssay Systems offers assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. (biosciregister.com)
  • PC12 rat pheochromocytoma cells were cultured in F-12K medium with 2.5% fetal bovine serum, 15% horse serum, and 1% penicillin/streptomycin. (moleculardevices.com)
  • The culture medium for the assay contained 3 percent fetal bovine serum, 100 6-TG resistant cells and 4x10(5) 6-TG wild type cells. (cdc.gov)
  • After co-culture, the completed assays are scored for visual phenotypes, such as growth or inhibition. (nih.gov)
  • In the V79 assay, the amount of cell killing that occurred when toxic 6-thioguanine (154427) (6-TG) monophosphate from wild type cells was transferred to 6-TG resistant cells, was an indication of cell to cell communication inhibition. (cdc.gov)
  • A total of 1795 influenza viruses were tested for oseltamivir-resistance using a fluorometric neuraminidase inhibition assay. (who.int)
  • By relating the amount of ATP to the number of viable cells, the assay has wide applications, ranging from the determination of viable cell numbers to cell proliferation to cell cytotoxicity. (sigmaaldrich.com)
  • A parallel cytotoxicity assay was also run without the 6-TG wild type cell. (cdc.gov)
  • The cytotoxicity analysis was performed using the MTT assay. (bvsalud.org)
  • Review the oxidative stress marker and assay guide to learn about more assays for oxidative stress. (abcam.com)
  • 2005). These fungi produce sever- lected in the present study by enrichment culture, using kraft al extracellular peroxidases, laccases, and additional oxidative lignin and kraft pulping lignin-enriched streams. (lu.se)
  • It has previously been shown that scrib KD MDCK cells are eliminated when co-cultured with wild-type MDCK cells through cell death and delamination (see ref. 23 and Supplementary Fig. 1a and Supplementary Movie 1 , left), while monocultures of scrib KD cells are viable (see ref. 23 and Supplementary Fig. 1b and Supplementary Movie 1 , right). (nature.com)
  • Growth rate (doubling time) profiles showed that scrib KD cells in pure cultures divide, albeit at a reduced rate, to reach a steadily maintained number ( Supplementary Fig. 1d ), whereas under competing conditions, their numbers collapse following initial growth ( Fig. 1a ). (nature.com)
  • Interestingly, we found that the growth rate of scrib KD cells is not affected by conditioned medium from competing cultures ( Fig. 1b and Supplementary Fig. 1c ). (nature.com)
  • MyeloCult™ media have been developed for the long-term culture of hematopoietic progenitor cells in association with adherent stromal cells. (stemcell.com)
  • In this assay hematopoietic cells are cultured in MyeloCult™ medium with supportive human or mouse stromal cell layers and tested for their ability to generate myeloid clonogenic progenitors for several weeks as assayed by replating the cells in a colony-forming unit (CFU) assay using MethoCult™ medium. (stemcell.com)
  • Sodium-potassium ratio: Another kind of assay practices the examination of the ratio of potassium to sodium in cells to serve as an index of viability. (wikipedia.org)
  • Assays such as these contain a stable enzyme common in all cells that can be readily detected when cell membranes are no longer intact. (wikipedia.org)
  • Functional: Assays of cell function will be highly specific to the types of cells being assayed. (wikipedia.org)
  • Red blood cells have been assayed in terms of deformability, osmotic fragility, hemolysis, ATP level, and hemoglobin content. (wikipedia.org)
  • Genomic and proteomic: Cells can be assayed for activation of stress pathways using DNA microarrays and protein chips. (wikipedia.org)
  • In addition, after the overexpression or knockdown of PAK3 in cells, the proliferation, migration and invasion abilities of these cells were assessed by Cell Counting Kit-8 (CCK-8), wound healing and Transwell assays, and the results were confirmed in vivo experiments in mice. (jcancer.org)
  • Naturally secreted from the deep sea copepod Gaussia princeps (3) (Figure 1), Gaussia Luciferase (GLuc) was first used in a sensitive analytical assay in 2002 (4) and later commercialized by NEB for expression in mammalian cells. (neb.com)
  • CHO cells stably expressing either a Gq or Gi coupled GPCR were plated at a cell density of 25K per well in 96 well half area tissue culture treated plates and cells were allowed to attach for 4h at 37°C. After 4h media was removed and replaced with 45 μL of DMEM/F12 w/o FBS to allow quiescence of the cells. (bmglabtech.com)
  • Cells were incubated for 18h and then the assay performed. (bmglabtech.com)
  • Following the incubation period, culture medium was removed and cells lysed with lysis buffer. (bmglabtech.com)
  • After 3 h exposure, the cells were separated from the exposure medium by centrifugation (5 min, 365 g). (europa.eu)
  • After a second centrifugation step, HBSS was removed and cells were resuspended in 5 mL culture medium and incubated for another 20 - 22 h (24 h fixation time). (europa.eu)
  • Cells (1 x 10(5)/mL) were cultured with or without 3 µM As2O3 for 48 h. (scielo.br)
  • In particular ExoTEST is a successful platform for exosomes quantification and characterization from small amount of human biological fluids or cell media and it may be exploited to identify exosomes released by cancer cells in the plasma and urine of tumor patients in various disease conditions. (immpact-international.org)
  • In addition, DNA extracted from bulks of cells formed by harvesting colonies from the serial dilution agar plates of a variety of culture media was used to profile the "cultivable" community. (who.int)
  • 10. Belemtougri RG, Constantin B, Cognard C, Raymond G, Sawadogo L. Effects of two medicinal plants Psidium guajava L. (Myrtaceae) and Diospyros mespiliformis L. (Ebenaceae) leaf extracts on rat skeletal muscle cells in primary culture. (bvsalud.org)
  • Fluorescent labels are stable for months and deliver high sensitivity, and the diversity in available dyes gives nearly unlimited possibilities in assay design. (labonline.com.au)
  • Learn how to master the challenges of cell-based fluorescence assays and improve the quality and sensitivity of your assays. (labonline.com.au)
  • This product is manufactured by BioVision, an Abcam company and was previously called K274 Total Antioxidant Capacity (TAC) Colorimetric Assay Kit. (abcam.com)
  • This Product is also available with Serum Cat# M36043-14PNS This product would require pre-coated flasks with Human Osteoblast Cell Culture Extra-cellular Matrix Cat#. (celprogen.com)
  • Published methods for vitamin B12 assay are available in the following references. (kcl.ac.uk)
  • Responses for many GPCRs such as Gi coupled GPCRs are not easily measured in current assay formats and other methods have been investigated as alternative readout. (bmglabtech.com)
  • Conventional hospitals in Egypt to implement sound 1 mL aliquot was used directly for the methods for the identification of S. au- infection prevention and control pro- LAMP assay and DCDD test. (who.int)
  • Total Antioxidant Capacity Assay Kit ab65329 can measure either the combination of both small molecule antioxidants and proteins, or small molecules alone in the presence of our proprietary Protein Mask. (abcam.com)
  • In the total antioxidant capacity assay protocol, the Cu2 + ion is converted to Cu + by both small molecule and protein antioxidants. (abcam.com)
  • Cell-based assays are giving us deeper insight into cellular mechanisms in a true biological context, and fluorescence assays are playing a leading role. (labonline.com.au)
  • Hence this type of assay has the ability to measure cellular changes activated by Gs, Gi and Gq coupled receptors giving potential to use a single assay format for screening. (bmglabtech.com)
  • Here a microplate reader equipped with a flash lamp is used to detect ERK phosphorylation using an AlphaScreen ® SureFire ® proximity-based assay. (bmglabtech.com)
  • In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the validation process include: cross-reactivity, dilution linearity, kit stability, and sample behavior (e.g. detectability and stability). (sigmaaldrich.com)
  • This paper presents a protocol for co-culture interaction assays that are inexpensive, scalable to large sample numbers, and easily adaptable to numerous experimental designs. (nih.gov)
  • To evaluate the accuracy of the HPV-Risk assay within VALGENT-4, relative to clinically validated comparator HPV tests. (nih.gov)
  • Reveal for Histamine (PN 9548) is a qualitative lateral flow assay intended for visual screening of histamine at 50 parts-per-million (ppm) in scombroid species of fish, such as tuna and mahi-mahi. (neogen.com)
  • Moreover, it could be used to complete the culture independent study of microbial diversity to give information on concentration ratios among species occurring in a particular environment and can be proposed for rapid identification of dominant microorganisms in alternative to traditional tools. (who.int)
  • Examples of this type of assay include propidium iodide, trypan blue, and 7-Aminoactinomycin D. Mitochondrial activity or caspase: Resazurin and Formazan (MTT/XTT) can assay for various stages in the apoptosis process that foreshadows cell death. (wikipedia.org)
  • Primary cultured HTFs were incubated with transforming growth factor β1 (TGF-β1) alone or combined with nintedanib, and cell proliferation and migration were measured by cell counting kit-8 (CCK8) and the scratch wound assay, respectively. (molvis.org)
  • Plates were incubated for 24 hours at 37°C. Upon completion of compound incubation, culture medium plus compound in the wells was replaced with a 100 nM solution of MitoTracker Deep Red FM (MTDR) diluted in culture medium. (moleculardevices.com)
  • Lymphocytes (0.4 mL blood of a healthy male donor was added to 5 mL or 4.8 mL culture medium, without and with metabolic activation respectively and 0.1 mL (9 mg/mL) Phytohaemagglutinin) were cultured for 48 h and thereafter exposed to selected doses of test substance for 3 h, 24 h and 48 h in the absence of S9-mix or for 3 h in the presence of S9-mix. (europa.eu)
  • The results of a study that evaluated the Chinese-hamster V79 cell metabolic cooperation assay were presented. (cdc.gov)
  • Five concentrations of each test chemical were used in the metabolic cooperation assay. (cdc.gov)
  • The authors conclude that since the promotion of tumors is complex and largely unknown, more experimentation is needed to understand the role of cell to cell communication and the usefulness of the metabolic cooperation assay. (cdc.gov)
  • The VALidation of HPV GENoyping Tests (VALGENT) framework is designed for comparison and clinical validation of HPV assays. (nih.gov)
  • HPV-Risk assay was performed on DNA extracted panel samples (n=1,295), blinded to clinical data, cytology results, and results from other HPV assays evaluated in VALGENT-4. (nih.gov)
  • Any assay development effort requires testing, evaluation, and validation of the assays with live or inactivated spiking materials in appropriate matrices relevant to the environments in which the assays are intended to be used (e.g., aerosol collection filters, soils, or clinical matrices). (cdc.gov)
  • Therefore, if the clinical suspicion is high yet the KOH result is negative, a fungal culture should be obtained. (medscape.com)
  • We constructed a genetically inactivated or attenuated strain containing relevant molecular assay targets and tested to compare assay performance using this strain to the historical data obtained using irradiation-inactivated virulent spores. (cdc.gov)
  • The AlphaScreen ® assay uses the diffusion of singlet state oxygen from Donor to Acceptor beads. (bmglabtech.com)
  • These features make these luciferases ideal for repeated assays, sensitive measurements in miniaturized assays and single cell applications. (neb.com)
  • Establish stromal cell layers and culture hematopoietic progenitors in "Dexter-type" long-term bone marrow cultures. (stemcell.com)
  • DCM assays were sensitive enough to separate workers exposed to 10 ppm from non-exposed workers. (who.int)
  • A test organism is cultured on one side of each well and first incubated in monoculture. (nih.gov)
  • Cultured peripheral human lymphocytes were used as test system. (europa.eu)
  • Lymphocytes were cultured for 48 ± 2 h and thereafter exposed in duplicate to selected doses of test substance for 3 h in the absence and presence of S9-mix. (europa.eu)
  • Fifteen minutes after the test chemicals were added, 6-TG was added to the culture medium to a final concentration of 10 microgram per milliliter (microg/ml). (cdc.gov)
  • After 3 days, the culture medium was replaced with fresh 10microg/ml 6-TG without test chemical. (cdc.gov)
  • A fungal culture is typically performed on Dermatophyte Test Medium (DTM). (medscape.com)
  • En parallèle, une identification microbiologique normalisée et un test de sensibilité à l'oxacilline par PCR visant l'amplification du gène Mec A ont été réalisés. (who.int)
  • Bacterial metabolism selected lignin model compounds (vanillin, vanillate, 4- hydroxybenzoate, p-coumarate, benzoate, and ferulate) was carried out with three of the Pseudomonas isolates and in addition with the strain Pseudomonas putida KT2440 from a Introduction culture collection. (lu.se)
  • Investigators must use their own laboratory assay system, which should be perfected a minimum of two weeks prior to fusion, specifically designed for their antigen and experimental application. (mayo.edu)
  • ry and non-respiratory samples received at Direct microscopy, culture on Lowen- our TB laboratory. (who.int)
  • This co-culture approach is applicable to antibiotic discovery as well as culture-based microbiome research and has already been successfully applied to both applications. (nih.gov)
  • Applications: Capture and quantification of exosomes from human biofluids and culture media. (immpact-international.org)
  • This homogeneous assay is ideally suited for screening applications where assay automation is required. (fishersci.com)
  • These techniques can be used to assess the success of cell culture techniques, cryopreservation techniques, the toxicity of substances, or the effectiveness of substances in mitigating effects of toxic substances. (wikipedia.org)
  • The media were conditioned by placing the substances in contact with fresh culture medium for 24 h. (bvsalud.org)
  • All assay results were reported to the central VALGENT coordination institute for data and statistical analysis. (nih.gov)
  • There were no false positive results but 7 PCR-negative, culture-positive samples were, considered false negatives after reviewing medical records of patients. (who.int)
  • Cytolysis or membrane leakage: This category includes the lactate dehydrogenase assay. (wikipedia.org)
  • An assay of the ability of a cell line to adhere and divide may be more indicative of incipient damage than membrane integrity. (wikipedia.org)
  • Human gastric cancer cell lines AGS and MKN28 were cultured and transfected with miR-149 overexpression plasmid and its control or FOXC1 siRNA and its control. (hindawi.com)
  • However, there are a variety of common protocols utilized for further observation of viability using assays. (wikipedia.org)
  • One hundred metaphase chromosome spreads per culture were examined by light microscopy for chromosome aberrations. (europa.eu)
  • The tetrazolium aspect of this assay, which utilizes both positive and negative charges in its formula, promotes the distinction of cell viability in a specimen. (wikipedia.org)
  • Appropriate negative and positive controls were included in the first cytogenetic assay. (europa.eu)
  • Of the 866 samples tested, 87 (10.0%) were PCR-positive compared to 94 (10.9%) culture positive. (who.int)
  • Sur les 866 échantillons testés, 87 (10,0 %) ont donné un résultat positif à la PCR contre 94 (10,9 %) ayant donné une culture positive. (who.int)
  • Biopsies from antrum, body and incisure were negative for presence of H. pylori by culture and PCR, while all biopsies were positive for presence of H. bizzozeronii by PCR. (bvsalud.org)
  • Designed to support cell culture growth and biomanufacturing processes. (fishersci.com)
  • They could arise over a three- to five-day period or may all be ready on the same day, so it's important for the client lab to be prepared weeks in advance with working specific secondary screening assays of choice. (mayo.edu)
  • A fungal culture, which is often used as an adjunct to KOH for diagnosis, is more specific than KOH for detecting a dermatophyte infection. (medscape.com)