Enzyme-linkeELISAAntibodyFlow cytometryAntigensDetectionAntibodiesImmunosorbentSerodiagnosisChemiluminescentProteinsLysateDilutionsCytotoxicityFluorescenceBorreliaResearchersLateralLymeDetectsDiagnosticBurgdorferiCellularMoleculesIndirectSpecimensRabbitInfectionProtocolsTestHumanPrimerTechnologyAnalysisMultipleGuideImagesDevelopmentSpecificStudySystemDiseaseNegativeData

Enzyme-linke5

• Using an apoptosis-specific enzyme-linked immunosorbent assay, we showed that Acanthamoeba induces programmed cell death in brain microvascular endothelial cells. (asm.org)
• Because of our patient's young age, the absence of other risk factors and his history of arthralgia, we requested a serum enzyme-linked immunosorbent assay (ELISA) analysis for Lyme disease on the day of admission. (cmaj.ca)
• This study investigated the use of anti-ribosomal P antibodies in the diagnosis of cerebral complications of systemic lupus erythematosus using two different methods-western blotting and enzyme-linked immunosorbent assay. (hkmj.org)
• Elevation of the level of anti-ribosomal P antibody, as measured by enzyme-linked immunosorbent assay, was 99% specific for systemic lupus erythematosus in 44% of patients with systemic lupus erythematosus that was not associated with cerebral involvement. (hkmj.org)
• The expressions of TLR4, NF-κ B and pro-inflammatory cytokines in the cortical were determined by real time polymerase chain reaction (RT-PCR), western blot, immunohistochemistry, or enzyme-linked immunosorbent assay (ELISA). (karger.com)

ELISA5

• Utilizing this substrate-free assay, researchers can achieve equivalent sensitivity to traditional chemiluminescence and fluorescence-based assays, while consolidating western blot and ELISA applications onto a single reader. (moleculardevices.co.jp)
• Learn about the Conferma ® ELISA development and manufacturing methods that provide strong sample detection and long-term assay and lot consistency, giving you confidence in your research. (sigmaaldrich.com)
• This article offers 4 popular ELISA protocols: Sandwich ELISA protocol, Phosphorylation Assay Procedure, EIA Assay Procedure, & Cell-based Assay Procedure. (sigmaaldrich.com)
• Levels of collagen, an indicator of fibrogenicity, were evaluated by ELISA and western blotting. (cdc.gov)
• Wenn der Patient in Europa unterwegs gewesen ist, wo unterschiedliche Stämme von Borrelien häufig sind, ist ein C6-Peptid-ELISA ein genauerer Bestätigungstest als die Western-Blots, die speziell für B burgdorferi entwickelt wurden, welches der häufigste Erreger in den Vereinigten Staaten ist. (medscape.com)

Antibody10

• Serum samples that are repeatedly reactive in the EIA for HIV-1 antibody are then retested with a supplemental and more specific test, the most common of which is the Western blot (1-3). (cdc.gov)
• In situ proximity ligation assays (isPLA) has been applied to antibody validation in human tissues with various advantages over IHC, including increased detection specificity, decreased unspecific staining, and better localization. (wikipedia.org)
• The most important update is revision of the laboratory criteria for a confirmed case, which addresses the development of new diagnostic testing algorithms that do not use the Western blot or immunofluorescence HIV antibody assays. (cdc.gov)
• ELISAs that use direct detection methods require an immobilized antigen that is bound directly to the surface of an assay plate or indirectly by a capture antibody, followed by an antigen-specific primary antibody conjugated to an enzyme, and the detection substrate. (sigmaaldrich.com)
• In addition to direct and indirect detection methods, capture or "sandwich" assays use an additional antigen-capturing antibody that is first attached to the microplate surface, followed by the use of both a primary and an enzyme-conjugated secondary antibody, similar to the indirect method previously described. (sigmaaldrich.com)
• The aim of this study was designing a Western blotting method for HSV1 antibody detection, using the purified virus by sucrose density gradient centrifugation procedure. (ac.ir)
• Use Anti-p300 CT Antibody, clone RW128 (Mouse Monoclonal Antibody) validated in Chimmunoprecipitation, immunoprecipitation, western blotting to detect p300 CT also known as KAT3B. (fishersci.com)
• Fluorophore-linked immunosorbent assay: FITC Antibody [NB100-64617] - FLISA plate was coated with Goat Anti-Human IgG-FITC and Goat Anti-Human IgG-BIOT. (novusbio.com)
• Western blots using CYP3A4 antibody demonstrated a dose responsive increase from 0.5 to 1 µM followed by decreasing responses at higher concentrations. (cdc.gov)
• Results for assays commonly marketed for detecting antibody to B burgdorferi are subject to misinterpretation, and medical practitioners should understand that the tests have limitations. (medscape.com)

Flow cytometry2

• The in vitro effects of the drugs on CRC cells were tested using CCK8 and flow cytometry assays. (lu.se)
• RNA-Seq, Western blotting, qRT-PCR and flow cytometry were used to identify the underlying molecular mechanisms. (lu.se)

Antigens4

• Antibodies to the polymerase (POL) gene products (p31, p51, and p66) are also commonly detected if these antigens are present on the Western blot strips. (cdc.gov)
• In fact, the true gp120 was shown to be absent from some commercial Western blot antigens. (cdc.gov)
• The purified viruses used as antigens in Western blotting assay. (ac.ir)
• Al- direct demonstration or cultivation of the though a number of papers have reported causative organism, remain the definitive the existence of circulating antigens and laboratory diagnostic method for VL, sero- immune complexes in VL [ 16-19 ], none of diagnostic methods are needed to over- them has led to a useful antigen detection come the diagnostic difficulties assay for VL. (who.int)

Detection6

• Although the overall sensitivity and specificity of the Western blot for detection of antibodies to the various viral proteins are high, there has been substantial debate regarding the interpretive criteria. (cdc.gov)
• Pre-optimized for the ScanLater™ Western Blot Detection Cartridge, this assay kit enables the first western blot detection capability in a microplate reader. (moleculardevices.co.jp)
• Proximity ligation assay (in situ PLA) is a technology that extends the capabilities of traditional immunoassays to include direct detection of proteins, protein interactions, extracellular vesicles and post translational modifications with high specificity and sensitivity. (wikipedia.org)
• Typically performed in multi-well microtiter plates, ELISAs are a molecular biology assay commonly used for the detection and quantification of diverse molecules, including peptides, proteins, and antibodies. (sigmaaldrich.com)
• An overview of the principles and applications of Western blotting for protein detection. (sigmaaldrich.com)
• Detection of human PPIP5K2 by western blot of immunoprecipitates. (thermofisher.com)

Antibodies5

• Antibodies to the HIV-1 major group-specific antigen (GAG) protein p24, and its precursor p55, are the earliest detected after infection by Western blot and tend to decrease or become undetectable with onset or progression of clinical symptoms (4-9). (cdc.gov)
• Blots were incubated with the ScanLater Eu-labeled Anti-rabbit Secondary Antibodies. (moleculardevices.co.jp)
• Western blotting detected anti-ribosomal P antibodies with a 90% specificity for systemic lupus erythematosus in 56% of patients with the disease. (hkmj.org)
• We conclude that the measurement of anti-ribosomal P antibodies by western blotting is helpful in the diagnosis of cerebral lupus in selected patients. (hkmj.org)
• Anti- B burgdorferi assays commonly yield false-positive results because of cross-reactive antibodies associated with autoimmune diseases or with spirochetal rickettsial, ehrlichial, or bacterial (eg, Helicobacter pylori ) infections. (medscape.com)

Immunosorbent1

• The enzyme-linked immunosorbent spot (ELISpot ) assay enables visualization of multiple secretory products from a single responding cell. (sigmaaldrich.com)

Serodiagnosis1

• Title : Interpretation and Use of the Western Blot Assay for Serodiagnosis of Human Immunodeficiency Virus Type 1 Infections Corporate Authors(s) : Centers for Disease Control (U.S.) Published Date : July 21, 1989 Source : MMWR. (cdc.gov)

Chemiluminescent1

• This video will show you how to adjust the image appearance of your chemiluminescent Western blot with Image Studio 4.0 Software on the C-DiGit Blot Scanner. (licor.com)

Proteins3

• The Western blot assay is a method in which individual proteins of an HIV-1 lysate are separated according to size by polyacrylamide gel electrophoresis. (cdc.gov)
• Thus, HERA is a novel cellular assay that can be used to predict how FcRn-binding proteins are rescued from intracellular degradation. (nature.com)
• The levels of peroxisome proliferator‑activated receptor gamma coactivator (PGC)‑1α/nuclear factor erythroid‑2‑related factor 2 (Nrf2) signalling, oxidative stress and pyroptosis‑related proteins were detected by western blotting. (spandidos-publications.com)

Lysate2

• However, in a recent study, the protein with a mobility of 160 kilodaltons (kd) present in commercially available Western blots and in viral lysate antigen preparations was identified as a multimer of the gp41 protein (10,11). (cdc.gov)
• Western blot sample preparations, including lysis buffers, lysate from cell culture, lysate from tissues and determination of protein concentration. (abcam.com)

Dilutions1

• Diluted sera (1:100, and 1:200 dilutions) used in Western blotting and two-fold dilutions of the sera applied in virus neutralization test.Five of twenty seven samples were negative in Western blotting and the same results obtained in virus neutralization test. (ac.ir)

Cytotoxicity2

• Experiments including photography of fluorescence in HSV-1g or plaque formation by HSV-1f, western blot assays, real-time RT-PCR assays, cytopathic effect inhibition assays, cytotoxicity assays, and viral absorption and penetration assays were performed to explore the antiviral effect and mechanism of the compounds. (molvis.org)
• Cytotoxicity was measured using the tryphan blue assay and lactate dehydrogenase (LDH) assay. (cdc.gov)

Fluorescence2

• The ScanLater™ Western Blot Assay Kit is a time-resolved fluorescence (TRF)-based assay optimal for quantitating as little as femtogram protein samples. (moleculardevices.co.jp)
• Importantly, viral absorption and penetration assays showed that the relative fluorescence intensity of HSV-1g was significantly reduced by SPS in a dose-dependent manner in the absorption test, but no change was observed in the penetration test. (molvis.org)

Borrelia1

• The next day, the assay showed elevated Borrelia immunoglobulin G (IgG) (498 U/mL) and immunoglobulin M (IgM) (2.7 U/mL) levels, and a positive IgG and IgM Borrelia immunoblot, confirming the diagnosis of Lyme disease. (cmaj.ca)

Researchers1

• Researchers use fluorescent western blotting to quantify multiple targets on a single blot. (the-scientist.com)

Lateral1

• Description Lateral motion immunochromatographic assay that adopted twin color system. (ivaskalab.com)

Lyme1

• Da Western-Blot-Tests notwendig sind, um falsch-positive Ergebnisse bei EIA oder IFA auszuschließen, aber nicht erforderlich werden, wenn die ersten Tests negativ sind, sollten Antikörper-Titer bei Lyme-Borreliose immer mit einem Reflex-Bestätigungstest beauftragt werden. (medscape.com)

Detects2

• The results show incomplete cross-reactivity between the two assays: the G. spinigerum-antigen-based immunoblot apparently only detects Asian gnathostomiasis caused by G. spinigerum, whereas the G. binucleatum-antigen-based immunoblot is apparently capable of detecting American as well as Asian gnathostomiasis. (unibas.ch)
• [ 3 ] Also available is a polymerase chain reaction (PCR) assay that detects B burgdorferi DNA. (medscape.com)

Diagnostic1

• KAtex est un simple supplément pour le diagnostic de la leishmaniose viscérale, en particulier sur le terrain, et un test complémentaire pour le diagnostic de la leishmaniose viscérale dans les cas à frottis négatif ayant des résultats positifs au test d'agglutination directe. (who.int)

Burgdorferi1

• After an active infection, anti- B burgdorferi can be detected on serologic assays for many years. (medscape.com)

Cellular1

• Documented experience working with cellular and biochemical assays related to the assessment of anticancer, anti- inflammatory and anti-EMT, and ROS-related activities in live and fixed cells. (lu.se)

Molecules1

• Such assays can detect molecules of interest at the pg/mL level and are critical for both basic research and disease research application needs. (sigmaaldrich.com)

Indirect1

• Western blot or indirect immunofluorescence assay test)" ( 5 ). (cdc.gov)

Specimens1

• HIV assays : operational characteristics (Phase I). Report 13: Urine specimens, oral fluid (saliva) specimens. (who.int)

Rabbit1

• Demonstrates stability of europium-labeled anti-rabbit IgG blots after 7 scans on the SpectraMax Paradigm Reader. (moleculardevices.co.jp)

Infection1

• The purpose of this report is to provide guidance for interpreting Western blot test results and their use in diagnosing HIV-1 infection. (cdc.gov)

Protocols1

• Sign up for emails and access exclusive application notes, protocols, and assay tips-all built on scientific expertise. (licor.com)

Test5

• To date, only one commercial Western blot test (Du PontPr) has been licensed by the Food and Drug Administration (FDA). (cdc.gov)
• The currently licensed Du Pont Western blot test specifies that the test result should be interpreted as positive only when the detected bands include p24 and p31, and gp41 or gp120/160 (12) (see Table 2). (cdc.gov)
• If results are positive, a Western blot test with multiple bands of identity should also be performed. (medscape.com)
• Der Western-Blot-Test wird nur dann durchgeführt, wenn in Schritt 1 die Testergebnisse positiv oder nicht eindeutig sind. (medscape.com)
• wenn Anzeichen und Symptome seit mehr als 30 Tage bestehen, findet nur der Western-Blot-Test für den IgG-Wert statt. (medscape.com)

Human2

• Exposure of human hepatocytes to doses of fipronil (5-amino-1-[2,6-dichloro-4-(trifluoromethyl)phenyl]-4-[(trifluoromethyl) sulfinyl]-1H-pyrazole-3-carbonitrile) ranging from 0.1 to 25 µM resulted in a dose dependent increase in CYP1A1 mRNA expression (3.5 to approximately 55-fold) as measured by the branched DNA assay. (cdc.gov)
• Cytotoxic effects of fipronil and fipronil sulfone were examined using the adenylate kinase and the trypan blue exclusion assays in HepG2 cells and human hepatocytes. (cdc.gov)

Primer1

• Predesigned TaqMan Assays Applied Biosystems TaqMan predesigned assays contain everything you need: TaqMan probe and PCR primer sets formulated to work right out of the box. (ivaskalab.com)

Technology1

• Discover how capillary-based western blot technology can take protein analysis into the 21st century. (the-scientist.com)

Analysis2

• Moreover, the activation of pIGF-1R, pAKT, and pS6 was examined by Western blot analysis. (hindawi.com)
• Review tips to guide all stages of western blotting, from sample preparation to imaging and analysis. (the-scientist.com)

Multiple1

• Another variation of in situ PLA includes a multiplex PLA assay that makes it possible to visualize multiple protein complexes in parallel. (wikipedia.org)

Guide2

• Troubleshoot and optimize ELISAs using this guide that includes solutions to some of the most common sources of problems for assay development. (sigmaaldrich.com)
• Download the complete western blot guide. (abcam.com)

Images1

• In this webinar, Aldrin Gomes will reveal how to obtain reproducible, publication-ready western blot images. (the-scientist.com)

Development1

• A robust, automated western blot system accelerates immunotherapy development. (the-scientist.com)

Specific1

• Measure and compare the activity of proteases with this non-specific protease activity assay using casein. (sigmaaldrich.com)

Study1

• Furthermore, this study presented evidence that the reaction observed against the gp120 on certain Western blots may have resulted in part from a reaction with a multimeric form of the gp41. (cdc.gov)

System2

• Results demonstrate the signal stability of the ScanLater Assay System. (moleculardevices.co.jp)
• Comparable result using ScanLater Western Blot System vs. chemiluminescence western blot (Stanford University). (moleculardevices.co.jp)

Disease1

• The US Centers for Disease Control and Prevention (CDC) recommendations are that Western blot should be used to confirm equivocal and positive results before the results are reported. (medscape.com)

Negative1

• However, we repeatedly experienced that sera from patients with clinically suspected American gnathostomiasis gave negative results in this assay. (unibas.ch)

Data1

• In this webinar, R. Hal Scofield discusses hints and tips on how to generate clean and reproducible western blot data, even in tricky situations. (the-scientist.com)