• These studies were accomplished by performing biorheological testing, Northern blot analysis and RNase protection assay. (stanford.edu)
  • The isolated RNA is suitable for Northern blots, dot blot hybridization, in-vitro translation, RNase protection assays and poly (A + ) selection. (gbiosciences.com)
  • The purified RNA is free from inhibitors and is concentrated into small elution volumes ideal for use in a variety of downstream applications including PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS. (norgenbiotek.com)
  • These applications include next-generation sequencing, transfection, real-time PCR, measuring yields of RNA preparations for microarray experiments, reverse transcription PCR (RT-PCR), differential display PCR, northern blot analysis, S1 nuclease assays, RNase protection assays, and cDNA library preparations. (thermofisher.com)
  • The RiboGreen reagent is ideal for fast and accurate measurement of RNA before generating cDNA, northern blot analysis, S1 nuclease assays, RNase protection assays, reverse transcription PCR, and differential display PCR. (thermofisher.com)
  • Relative to other techniques for measuring mRNA, such as Northern blot analysis, RNAse protection assays, or in situ hybridization, RT-PCR is significantly more robust at detecting the RNA transcript of any gene regardless of its relative abundance. (stratech.co.uk)
  • Gene probes are used in various blotting and in situ hybridization (ISH) techniques for the detection of nucleic acid sequences in food industry, environmental, medical, and veterinary applications to improve the specificity of the analyses. (enzolifesciences.com)
  • The functional roles of the two TNFRs in mediating NF-$\kappa$B activation, and inducing IL-2R$\alpha$ chain (CD25) and ICAM-1 (CD54) expression, were examined using receptor-specific antisera and TNF mutants, Northern blot analysis, gel shift assay and flow cytometry on human NK-like YT cells. (jefferson.edu)
  • Electrophoretic mobility shift assays were performed in order to examine nuclear factor (NF)-κB and activator protein (AP)-1 binding activities. (ersjournals.com)
  • Gel shift assays or electrophoretic mobility shift assay (EMSA) protocols usually require labelling DNA by radioisotope, digoxygenin, or biotin. (selectscience.net)
  • Furthermore, electrophoretic mobility shift assays demonstrate a direct interaction of s479 with the target znuC1 mRNA. (uni-stuttgart.de)
  • In all, 4311 men were tested for HIV antibodies by enzyme-linked immunosorbent assay, with confirmation by Western blot assay. (duke.edu)
  • These cells were characterised by PCR, immunocytochemistry, electron microscopy, and Western and Northern blot analyses. (ersjournals.com)
  • Northern blot analyses indicated that the mRNAs of p50 and p52, but not that of p65 RelA, are induced by TNF or either of the two TNFR antisera. (jefferson.edu)
  • We performed northern blot analyses for the guide RNA truncations and found increased levels of expression for the longer tracrRNA sequences, suggesting that improved target cleavage was at least partially due to higher sgRNA expression or stability ( Fig. 1c ). (cdc.gov)
  • Upregulation of these genes in the deletion strain was confirmed by northern blot and proteome analyses. (uni-stuttgart.de)
  • RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction. (delos.info)
  • Using the SURVEYOR nuclease assay 13 , we assessed the ability of each Cas9-sgRNA complex to generate indels in human embryonic kidney (HEK) 293FT cells through the induction of DNA doublestranded breaks (DSBs) and subsequent nonhomologous end joining (NHEJ) DNA damage repair (Online Methods). (cdc.gov)
  • Därefter användes real-tids PCR, en metod som anger mRNA nivåer i en vävnad, för att upptäcka skillnader mellan individerna. (5dok.org)
  • Vi fann ett antal samband mellan mRNA uttryck och etanolintag i speciellt prefrontala cortex, en region i hjärnan som är inblandad i bl.a. bearbetning av nya intryck, minne och kontroll av beteende. (5dok.org)
  • The expression of lymphotoxin (LT) mRNA and cytokine in human tonsillar B cells and B cell lines was examined by Northern blots and cytotoxicity assays, respectively. (escholarship.org)
  • Northern blotting demonstrated that quercetin inhibited LPS-induced TNF-α mRNA, but did not alter its half-life. (elsevierpure.com)
  • As new miRNAs are discovered, custom microarray chips accommodate the entire set of known miRNAs on a single chip allowing for rapid and reliable high-throughput analysis that have been validated on more widely accepted platforms such as qPCR and Northern blots. (bitesizebio.com)
  • The circBACH1 and miR-217 expression levels were detected using quantitative real-time PCR (RT-qPCR) and Immunohistochemistry (IHC) assays in BC tissues and precancerous tissues of BC patients. (biomedcentral.com)
  • Eph continues to be expressed in the larval CNS and imaginal discs, as well as pupal and adult stages as assayed by Northern blot analysis. (sdbonline.org)
  • In order to identify the ligand-binding domain(s) of the two TNFRs, recombinant domain-deletion mutants of TNFRs were expressed and their binding capacities assessed by ligand blot analysis and Scatchard analysis. (jefferson.edu)
  • sp{125}$I-TNF-$\alpha$ saturation binding assay and Scatchard analysis showed that the transfected hTNFR-1, interacts with hTNF-$\alpha$ much more strongly than mTNFR-1 does. (jefferson.edu)
  • Northern blot analysis has shown widespread expression of EDG8 in rat tissues, with strongest expression in spleen and white matter tracts of the brain. (multispaninc.com)
  • Many new nucleic acid-based diagnostic tools or assays have been developed that allow analysis of DNA and RNA molecules in clinical samples. (enzolifesciences.com)
  • Methods: TGF-β2 expression was evaluated in LCC2 cells and tamoxifen-sensitive LCC1 cells by northern blot analysis. (elsevierpure.com)
  • RNA-analysis: native RNA may be analyzed by 3´-terminal pCp-labelling or Northern-Blot. (sysy.com)
  • PicoGreen reagent assays provide a rapid and simple fluorescence-based method for the accurate quantitation of dsDNA. (thermofisher.com)
  • The assay is many times more sensitive than conventional UV absorbance, and free nucleotides or short oligonucleotides of up to 6 bases do not interfere with the OliGreen ssDNA quantitation assay. (thermofisher.com)
  • Fast and convenient: single reagent "mix-and-measure" assay allows quantitation of free phosphate within 30 minutes. (wlsolutions.be)
  • Description: Our Phosphate Assay Kit (Fluorometric) measures total inorganic phosphate present in lysates, solutions, food, or biological samples in a 96-well fluorescence-based plate reader. (wlsolutions.be)
  • Assay design is also highly variable and can make the difference between PCR success and failure and also contributes to the reproducibility and sensitivity of an assay. (sigmaaldrich.com)
  • Sensitivity to tamoxifen was measured in a soft agarose colony- forming assay and in a xenograft model in nude and beige/nude mice. (elsevierpure.com)
  • The following protocol is for assays using 96-well plates. (wlsolutions.be)
  • This simple and convenient single-tube design works well when performing a small number of assays and is amenable to high-speed, high-throughput applications. (stratech.co.uk)
  • 10 - 20 µg antibody per assay are coupled to protein A or protein G sepharose in PBS at 4°C head over tail (several hours). (sysy.com)
  • Generally, non-specific interactions should be controlled with a parallel pull-down assay using protein A/G-sepharose without antibody. (sysy.com)
  • This study evaluated the diagnostic accuracy of a T solium cysticercosis antibody-detecting lateral-flow point-of-care assay (TS POC test) for the neuroimaging-based diagnosis of neurocysticercosis. (cdc.gov)
  • Evidence that intrauterine infection was caused by the RB51 vaccine strain, and not by field strains of B. abortus or by S19, included immunohistochemical staining specific for RB51 (negative for S19), RB51-specific titer of greater than 1:10,000 on experimental dot-blot assay measuring antibody to RB51, and RB51-specific DNA sequences identified by polymerase chain reaction (PCR). (cdc.gov)
  • Nuclease protection assay is a laboratory technique used in biochemistry and genetics to identify individual RNA molecules in a heterogeneous RNA sample extracted from cells. (wikipedia.org)
  • Nuclease protection assays are used to map introns and 5' and 3' ends of transcribed gene regions. (wikipedia.org)
  • Nuclease protection assay products are limited to the size of the initial probes due to the destruction of the non-hybridized RNA during the nuclease digestion step. (wikipedia.org)
  • DNA/RNA probe assays are faster and sensitive so that many conventional diagnostic tests for viruses and bacteria involving culturing of the organisms are being fast replaced by molecular probe assays. (enzolifesciences.com)
  • Characterization of the epidemiology and clinical features of these and other possible agents of viral hepatitis will await the development of diagnostic assays. (cdc.gov)
  • Molecular Diagnostic and Prognostication Assays for the Subtyping of Urinary Bladder Cancer Are on the Way to Illuminating Our Vision. (who.int)
  • PicoGreen reagent assays are routinely used for genotyping by allele-specific PCR, quantitating dsDNA samples before and after PCR amplification, and determining PCR amplification yields before sequencing. (thermofisher.com)
  • OliGreen reagent assays are routinely used for antisense oligonucleotides, aptamers, genomic DNA isolated under denaturing conditions, PCR primers, sequencing primers, and single-stranded phage DNA. (thermofisher.com)
  • These assays are now routinely used for monitoring or detecting, as well as to help decide which therapies would work best for patients. (enzolifesciences.com)
  • ABSTRACT Crude antigenic preparations from Setaria equina were used in ELISA and Western blotting to examine cross- reaction with human sera from areas endemic for bancroftian filariasis. (who.int)
  • ELISA et transfert Western afin d'étudier la réaction croisée avec des sérums humains en provenance de zones endémiques pour la filariose de Bancroft. (who.int)
  • Western blotting is an important technique used in cell and molecular biology. (delos.info)
  • BTI-CMe was properly transcribed and translated as indicated by northern and western blot, with a level of expression in transgenic wheat seeds up to 1.1% of total extracted protein. (upm.es)
  • The effects of PTX-induced exosomal circBACH1 on BC cell function were assessed using RNA Binding Protein Immunoprecipitation (RIP), dual luciferase reporter gene, tube formation, CCK-8, and Western Blot assays. (biomedcentral.com)
  • Phosphorylation of extracellular signal-related kinases 1 and 2 (ERK 1/2), p38 MAPK, and Jun N-terminal kinase/stress-activated protein kinase (JNK/SAPK), members of the MAPK family, was analyzed by western blotting. (elsevierpure.com)
  • Cysticercosis serum apDia antigen ELISAs and Western blot for LLGP and rT24h antigen were performed on all PWE and 2% of screen-negative patients. (cdc.gov)
  • Therefore no filtration of reagent is needed prior to assays, as is often required with other commercial kits. (wlsolutions.be)
  • Both Northern blotting and nanopore direct RNA sequencing were also applied to examine the reproducibility of noncanonical transcripts. (biomedcentral.com)
  • While culture tests can take days, molecular probe assays can be performed within a few hours or minutes. (enzolifesciences.com)
  • Northern blotting is a laboratory technique that produces similar information. (wikipedia.org)
  • Compatible with routine laboratory and HTS formats: assays can be performed in tubes, cuvettes or microplates, on spectrophotometers and plate readers. (wlsolutions.be)
  • Confidently detect more with Archer NGS assay solutions for your solid tumor, blood cancer, immune profiling, and genetic disease research. (idtdna.com)
  • To experimentally validate the synthesis of noncanonical transcripts under regular infection, Northern blotting was performed. (biomedcentral.com)
  • Otros autores sugieren que ese es el resultado de mutaciones en oncogenes, genes supresores y enzimas de la vía glucolítica o del metabolismo oxidativo mitocondrial (Myc, Akt, p53, HIF1-α). (researchgate.net)
  • Supershift assay identified that the nuclear NF-$\kappa$B complexes activated by TNF are P50/p50 and p50/p65 dimers. (jefferson.edu)
  • La aplicación de la tomografía de emisión de positrones (PET) en los servicios de medicina nuclear y radiología ha permitido usar la mitocondria como un organelo para el diagnóstico de cáncer cuantificando una mayor captación de glucosa por las células tumorales respecto del tejido no tumoral adyacente, mediante el uso del análogo radioactivo no metabolizable de la glucosa (18FDG, 18F-2-desoxiglucosa). (researchgate.net)
  • Non-specific amplification can be used to increase the miRNA sample, but when measuring expression levels, this means you'll lose the direct correspondence between total input sample and the amounts of miRNAs measured, introducing non-linearity in the assay. (bitesizebio.com)
  • reverse line blot (Innogenetics, Gent, Belgium). (cdc.gov)
  • Carolina, USA (J.A. Crump, A.B. Morrissey, N.M. Thielman, J.A. tifi ed as M. simiae by the INNO-LiPA reverse-line blot. (cdc.gov)
  • 12q14 (Sanfilippo syndrome): The diagnosis requires a specific lysosomal enzyme assay for glucosamine ( N -acetyl)-6-sulfatase (GNS) activity. (medscape.com)
  • The assays are many times more sensitive than conventional UV absorbance and can be optimized for different concentration ranges. (thermofisher.com)
  • This step significantly reduces background in pull-down assays. (sysy.com)
  • Functional integrity of BTI-CMe was confirmed by trypsin inhibitor activity assay. (upm.es)
  • Secreted TGF-β activity was quantified by use of an 125 I-TGF-β competitive radioreceptor assay. (elsevierpure.com)
  • In addition, Northern blotting was also employed to determine the regulatory features of noncanonical transcripts under different infection conditions, including different cells, multiplicities of infection (MOIs) and coronavirus strains. (biomedcentral.com)
  • In particular, the invention relates to microfluidic devices and their uses and methods for assaying cells. (justia.com)
  • METHODS: Using direct interviews, we studied five cohorts of 21-year-old men from northern Thailand who were conscripted into the army by a lottery in 1991, 1993, and 1995. (duke.edu)
  • It is for 1000 assays using 96-well plates (100 ul of sample volume) or 4000 assays using 384-well plates (25 l of sample volume). (wlsolutions.be)
  • Notable among these were a study of hard-metal workers in several small factories in northern Italy. (who.int)
  • You can view Archer assays alongside IDT's xGen™ NGS portfolio to find the best next generation sequencing solution for your lab. (idtdna.com)
  • In this assay, miRNAs in a heterogenous mixture of total cellular RNA are amplified using specific primers. (bitesizebio.com)
  • De ese modo, se recolectó un total de 106 documentos seleccionados a partir de la búsqueda bibliográfica en las bases de datos. (researchgate.net)