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  • antibody
  • The cardiac isoforms of cTnI and cTnT have additional unique N-terminal amino acid sequences, which allows for specific antibody and assay development and detection of each component in the bloodstream. (ahajournals.org)
  • Cell-based neutralizing antibody assays can be difficult to develop as the selection of the most suitable assay type (indirect or direct) and format depends on thorough understanding of the drug's mechanism of action. (intertek.com)
  • Through the intelligent and experienced application of cell-based neutralizing antibody assays, Intertek is the ideal partner for your biologic drug development or pharmacovigilance requirements for immunogenicity testing . (intertek.com)
  • Thermo Scientific
  • Measure TurboLuc™16 (Tluc16) luciferase activity in mammalian cells with a single reagent-addition step that is for high-throughput screening (HTS) applications using the Thermo Scientific™ TurboLuc™ Luciferase One-Step Glow Assay Kit. (fishersci.com)
  • Vectors
  • Browse the product groups below to find reporter assays, a comprehensive range of reporter vectors, luciferase-expressing cell lines and low-toxicity transfection reagents to ensure successful delivery of vectors to the cell line of your choice. (promega.com)
  • Membrane
  • The original version of the chick chorioallantoic membrane (CAM) angiogenesis assay was introduced by experimental embryologists 50 years ago, but it still represents a centerpiece for the study of organ development. (news-medical.net)
  • vivo
  • A majority of endothelial cell assays utilize human umbilical vein endothelial cells or bovine aortic endothelial cells - not because these types of cells are good representatives of vascular conditions in vivo , but primarily because they are relatively easy to harvest from large blood vessels. (news-medical.net)
  • The earliest of in vivo assays contained diffusion chambers with Millipore filters designed to visualize and monitor neovascularization of implanted tumors. (news-medical.net)
  • In the meantime, many in vivo assay methods have been developed that permit better quantification, and are also much easier to perform. (news-medical.net)
  • Thus corneal angiogenesis assay is still regarded as one of the best in vivo assays, and any vessels observed in the cornea after applying angiogenesis-inducing factors represent newly formed vessels. (news-medical.net)
  • variability
  • Reduce assay variability and improve predictability with Corning osteo assay surface, a ready-to-use synthetic surface made of an inorganic crystalline calcium phosphate coating that mimics living bone material. (corning.com)
  • detection
  • This firm also developed Click-iT EdU Microplate Assay that utilizes nucleoside analog EdU (5-ethynyl-2'-deoxyuridine) and a detection method that does not necessitate DNA denaturation. (news-medical.net)
  • The single cell gel electrophoresis assay ( SCGE , also known as comet assay ) is an uncomplicated and sensitive technique for the detection of DNA damage at the level of the individual eukaryotic cell . (wikipedia.org)
  • First, an attachment assay, which employs a colorimetric detection of bound cells, and second, a spreading assay, which employs phase contrast microscopy to measure the flattening of adherent cells. (springer.com)
  • parameters
  • Assay development and optimization are performed through careful selection of proper cell line, assay format, and positive control NAb, optimization of assay parameters, sample dilution or pretreatment, if necessary, and meticulous data processing and statistical analysis. (intertek.com)
  • therapeutic
  • ASSAY and Drug Development Technologies delivers access to the techniques and tools that enable new advances in early-stage screening, and lead to therapeutic compounds for drug discovery and development. (liebertpub.com)
  • Each assay must be target specific, reproducible, minimally affected by matrix effects, and be able to detect the NAb in the presence of the therapeutic. (intertek.com)
  • Our experts, based in Good Laboratory Practice (GLP) compliant laboratories, evaluate each therapeutic, conduct tailored assay development and ensure that each assay is optimized to suitably determine the presence of NAb with maximum possible level of drug tolerance. (intertek.com)
  • detect
  • Canon BioMedical, a wholly owned subsidiary of Canon USA, has launched a line of genotyping assays to detect SNPs, small insertions, and deletions in the human genome. (genomeweb.com)
  • researchers
  • At launch the firm will market 103 pre-designed assays as well as an "open" chemistry for researchers to customize, as reported by GenomeWeb. (genomeweb.com)
  • implementation
  • This Program supports implementation of innovative biological, biophysical or cell-based assays for novel biological targets or processes relevant to mental disorders. (nih.gov)
  • 4 For this reason, each laboratory should validate the accuracy and precision of its troponin assay both on initial implementation and also every few months to verify assay performance. (ahajournals.org)
  • functional
  • Use the osteo assay surface to enhance bone cell differentiation, functional analysis, and tartrate acide phosphate (TRAP) activity research, along with many other drug discovery applications. (corning.com)
  • Apart from fresh human hepatocytes, HepaRG cells are the most metabolically active liver cell line described to date and have the potential for use as a viable surrogate in many functional liver assays, including metabolism and clearance testing, with none of the drawbacks of limited availability and donor-to-donor variation. (sigmaaldrich.com)
  • laboratories
  • Laboratories must interact with clinicians to establish diagnostic cut points, achieve acceptable assay performance, and troubleshoot questionable findings. (ahajournals.org)
  • Chemicals
  • We provide intestinal transporter assays for small molecule formulations such as pharmaceuticals, industrial chemicals and consumer products. (sigmaaldrich.com)
  • reporter
  • The small size and bright luminescence of NanoLuc® Luciferase bring exquisite sensitivity to reporter assays and other bioluminescence applications. (promega.com)
  • Human
  • 24- or 96-multiwell BD FluoroBlok insert (with 3 μm pore size) coated with human fibronectin was developed by a company BD Biosciences, and also serves as a cell migration assay. (news-medical.net)
  • Soluble TRAP activity found in the medium human osteoclast precursor cells cultured for 5 days on the osteo assay surface. (corning.com)
  • cells
  • Basically, if you have more than a few assays to do and don't need to culture cells on the filter, I would go with Neuroprobe. (bio.net)
  • The comet assay (single-cell gel electrophoresis) is a simple method for measuring deoxyribonucleic acid (DNA) strand breaks in eukaryotic cells. (wikipedia.org)
  • RAW264.7 cells were grown for 7 days in the presence of RANKL on osteo assay surface. (corning.com)
  • the cells have grown up and the assay will be performed. (bio.net)
  • anyone
  • Does anyone know of good calcium binding assay Thanks Anne George -- ANNE GEORGE Northwestern University, Evanston, IL. (bio.net)
  • Greetings: Has anyone out there in net-land ever done the MPO assay? (bio.net)
  • concentration
  • Moreover, binding assays have been useful in examining the effects of drugs and disease on neurotransmitter receptors, and the procedure has been adapted for use as a simple and sensitive analytical method for measuring the concentration of neurotransmitters and drugs. (springer.com)
  • I want to make a stock solution of 1.0mM (or greater) and use it at a final concentration of 0.8mM in the assay. (bio.net)
  • activity
  • Assay regulatory element transcriptional activity in mammalian cell culture media and whole cell lysate with this highly sensitive flash-based luciferase kit. (fishersci.com)
  • Data from metabolic screening assays allow customers to identify metabolic liabilities early on and focus on the improvement of drug candidates through structure activity relationships. (sigmaaldrich.com)
  • However, there are currently no 'kits' for assaying activity (at least of the Jun kinases or p38/ CSBP/HOG). (bio.net)
  • means
  • The metabolic stability assays provide a means to measure the rate of disappearance of a test compound over time in either microsomal or hepatocyte incubations, and these data are used to calculate intrinsic clearance. (sigmaaldrich.com)
  • methods
  • One of the more powerful methods for this purpose has been the radioligand binding assay (Yamamura et al, 1978). (springer.com)
  • often
  • The lysis solution often used in the comet assay consists of a highly concentrated aqueous salt (often, common table salt can be used) and a detergent (such as Triton X-100 or sarcosinate ). (wikipedia.org)
  • It is often essential to measure the levels of the various hormones in the body, which may play a part in the control of ovulation.Why are hormone assays required Infertility treatment takes a holistic approach. (indiaparenting.com)
  • popular
  • Because of its simplicity and power, the radioligand binding assay has become one of the more popular tools in neurobiological research. (springer.com)