Treat cells with test compounds for a desired period of time (4-6 hours for Jurkat cells treated with staurosporine) to induce apoptosis. (aatbio.com)
In order to demonstrate the flexibility and verstality of the NovoCyte system for assessment of apoptotic events, we show three di fferent assays that can measure apoptosis in response to staurosporine, a broad kinase inhibitor known to induce apoptosis in many cell types. (accela.eu)
A recent study showed that another protein in silkworm hemolymph, storage protein 2 (SP2), can also inhibit staurosporine-induced HeLa cell apoptosis and ROS generation [ 8 ]. (hindawi.com)
Positive Control: Staurosporine-treated HeLa or Jurkat cell lysate. (thermofisher.com)
High-throughput3
Thus, experimental systems that integrate sensitive and high-throughput assays with physiological TME will strengthen the preclinical drug discovery process. (elsevierpure.com)
The TiterTACS Colorimetric Apoptosis Detection Kit is a TUNEL based assay utilizing specific in situ labeling technology that enables a 96 well microplate format for high throughput quantitative detection of apoptosis. (rndsystems.com)
Kinase inhibitor2
Staurosporine is a prototypical ATP-competitive kinase inhibitor in that it binds to many kinases with high affinity, though with little selectivity. (ncats.io)
Using this platform, we evaluated dose-dependent responses of tissue slices to the multi-kinase inhibitor, staurosporine, and cytotoxic agent, doxorubicin. (elsevierpure.com)
IC502
Staurosporine is also a potent GSK-3β inhibitor with a reported IC50 value of 15 nM. (ncats.io)
Sample analysis was performed on a FACSCanto II (BD Biosciences) with DIVA 8 software and histograms were generated using FlowJo v9 cytometry analysis software (Tree Star, Inc.). BRD inhibition/binding assays and profiling The half maximal inhibitory concentration (IC50) of each compound against BETs was determined by Reaction Biology Corp. using a chemiluminescent Alpha screen binding assay. (antiviralbiologic.com)
Etoposide2
China (12KJB320009), and the Research Project of the Technology and Technology Bureau of Suzhou City of P.R. examine the part of Ambra1 in apoptosis, Ambra1 knockdown cells were treated with staurosporine and etoposide. (rawveronica.com)
Protects against apoptosis induced by TNF or by chemical agents such as adriamycin, etoposide or staurosporine (PubMed:11162435, PubMed:11084335, PubMed:11865055). (qedbio.com)
Viability3
A cell viability assay demonstrated that SP2 could significantly enhance the viability of HUVEC. (hindawi.com)
To assess changes in tissue viability in response to drug treatment, we leveraged a biocompatible luminescence-based viability assay that enables real-time, rapid, and sensitive measurement of viable cells in the tissue. (elsevierpure.com)
Cell apoptosis and viability were measured by annexin-V and PI staining and MTT assays. (rawveronica.com)
Doxorubicin1
While spontaneous apoptosis was similar in ALK+ ALCL cell lines expressing either GzB or control shRNA, GzB shRNA-expressing cells were less sensitive to staurosporine or doxorubicin-induced apoptosis as evidenced by reduced PARP cleavage and decreased DNA fragmentation. (biomedcentral.com)
Biological activities1
Staurosporine was discovered to have biological activities ranging from anti-fungal to anti-hypertensive. (ncats.io)
Apoptotic4
Furthermore, both ELISA and flow cytometry results indicated that SP2 has anti-apoptotic effects on staurosporine-induced HUVEC apoptosis. (hindawi.com)
A set of in vitro tests performed by Dr. Christian Peters, at BIDMC, confirmed that activation of PAR1 by parmodulin 2 induces cytoprotective responses in endothelial cells by inhibiting apoptosis, normally induced by thrombin, TNF-α, and the apoptotic alkaloid staurosporine. (news-medical.net)
The fi rst assay uses Annexin V to bind exposed PS on cell membranes for labeling early apoptotic cells and the DNA binding dye, 7-AAD, for labeling late apoptotic cells. (accela.eu)
The second assay makes use of the fl uorescent dye JC-1, to measure the loss of mitochondrial membrane potential in early apoptotic cells. (accela.eu)
Inhibitors2
The binding of PKC betaII to actin was not inhibited by kinase inhibitors of PKC (sphingosine and staurosporine), suggesting that prior activation and/or substrate phosphorylation are not required for the interaction of PKC betaII with actin. (duke.edu)
The search for specific inhibitors is mainly performed in cell-based assays. (eurekaselect.com)
Cells were treated1
Human ML-1 myeloblastic leukemia cells were treated with 1 µM staurosporine and then harvested, fixed, and labeled according to the protocol in the TiterTACS In Situ Detection Kit (Catalog # 4822-96-K ) prior to colorimetric analysis. (rndsystems.com)
Inhibition2
The main biological activity of staurosporine is the inhibition of protein kinases through the prevention of ATP binding to the kinase. (ncats.io)
Staurosporine induces apoptosis by multiple pathways and that the inhibition of more than one kinase is responsible for its potent activity. (ncats.io)
Compounds1
CHEK2 NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target. (reactionbiology.com)
Flow1
Add 200 to 300 µL of Assay Buffer (Component B) to increase volume before analyzing the cells with a flow cytometer. (aatbio.com)
Maximal1
The TiterTACS Kit also provides TACS-Nuclease solution to generate positive controls from your own samples giving you a maximal value for the assay. (rndsystems.com)
Preclinical1
Because the mechanism of action of staurosporine is distinct from traditional anticancer drugs, this may warrant further preclinical evaluations of the antitumor potential of new staurosporine derivatives either alone or in combination with death ligands or conventional chemotherapeutic drugs. (ncats.io)
Cellular1
Because it mimics some of the neuroprotective effects of NGF and might blocks certain signals required to enhance cellular levels and/or beta amyloid processing, staurosporine might play a beneficial role in the treatment of Alzheimer's disease. (ncats.io)
Cell4
Additional studies have also shown that hemolymph from the silkworm ( Bombyx mori ) can inhibit insect and mammalian cell apoptosis that is induced by viruses and several chemical inducers, such as staurosporine, camptothecin, and actinomycin D [ 6 - 8 ]. (hindawi.com)
In this study, tumorigenic potential was assessed using anchorage-independent growth assays and spheroid-forming capacity, which was determined with ovarian cancer cell lines as well as primary ovarian cancers. (duke.edu)
for 5 min and resuspended in CelLytic M Cell Lysis Reagent (Sigma-Aldrich) containing Halt Protease Inhibitor Cocktail and Halt Phosphatase Inhibitor Cocktail (Thermo Scientific, Waltham, MA) and 5 mM EDTA at 4 C. Proteins concentrations were established with Bio-Rad Proteins Assay Reagent (Hercules, CA) and examples had been diluted with 1/3 quantity 4X SDS test buffer and warmed at 95 C for 5 min. (antiviralbiologic.com)
Assess1
We also performed caspase-1 activity assay and enzyme-linked immunosorbent assay to assess caspase-1 activity and IL-1β production, respectively. (biomedcentral.com)
Protein1
Staurosporine might be considered as a prototype neurotropic drug in view of its ability to induce neurite outgrowth and to increase tau protein levels. (ncats.io)
Concentration1
At higher concentration (1-20 nM), staurosporine also inhibits other kinases such as PKA, PKG, CAMKII and Myosin light chain kinase (MLCK). (ncats.io)
Measurement1
The table contains an overview of kinase assays that are available for measurement of target residence time measurement. (residencetimer.com)
Human1
The purified SP2 was then added to a culture medium for human umbilical vein ECs (HUVECs), which were exposed to staurosporine. (hindawi.com)
Activity2
The PKC activity increased by the activation of the passively sensitized mast cells was decreased by calphostin C, staurosporine and cromakalim, respectively. (kyobobook.co.kr)
The third assay quantifi es caspase activity. (accela.eu)
Show1
Using an actin-binding co-sedimentation assay, we show in vitro that PKC betaII, but not PKC betaI, binds to actin specifically. (duke.edu)
Chemical1
Staurosporine was discovered at the Kitasato Institute in 1977 while screening for microbial alkaloids using chemical detection methods. (ncats.io)