Assay rna stability. Medical search. Frequent questions

Ribonucleoprotein complex immunoprecipitation and biotinylated RNA pull-down assays demonstrated that PARP-14 forms a complex with the mRNA-destabilizing protein tristetraprolin (TTP) and a conserved adenylate-uridylate-rich element in the TF mRNA 3′ untranslated region. (imperial.ac.uk)
RNA sequencing, RNA pulldown and RNA immunoprecipitation were used to elucidate the underlying mechanism of VIM‑AS1 driving prostate progression. (spandidos-publications.com)
We performed nano-bead pull-down assays, liquid chromatography-mass spectrometry, computational modeling, and ribonucleoprotein immunoprecipitation analyses to identify MUFA-interacting proteins. (nih.gov)
Bioinformatic analysis, RNA-protein pull down, mass spectrometry, RNA immunoprecipitation, luciferase reporter assay and fluorescence in situ hybridization were performed to validate the HNRNPL/circFAM13B/IGF2BP1/PKM2 cascade. (biomedcentral.com)

Digestive symptoms in COVID-19 patients with mild disease for isolation and care at our hospital: 1 patient who severity: clinical presentation, stool viral RNA testing, and was asymptomatic, 2 whose symptoms had subsid- outcomes. (cdc.gov)
of viral RNA concentrations among specimen types. (cdc.gov)
The VA (viral associated) RNA is a type of non-coding RNA found in adenovirus. (wikipedia.org)
In addition, detection of viral RNA by reverse transcription--polymerase chain reaction (RT-PCR), usually in a throat swab or urine sample, and subsequent genotyping of strains is valuable for diagnosis and molecular epidemiology ( 2 ). (cdc.gov)
Accurate laboratory results for detection of IgM and viral RNA are dependent on proper collection, processing, shipment, and storage of clinical samples and use of accurate tests performed by a proficient laboratory. (cdc.gov)
DBS has been used for various epidemiologic studies for the detection of measles- and rubella-specific IgG and IgM antibodies and viral RNA ( 3--5 ). (cdc.gov)
In practice, the viral load is estimated from the number of copies of ribonucleic acid (RNA) HIV-1 per milliliter of plasma, determined with commercial molecular technic used to evaluate the effectiveness of ART. (scirp.org)
In the case of targeting viral RNA, specifically for the diagnosis of the ISA virus in infected tissues of fish, the use of the elongation factor 1 alpha (ELF1α) has been demonstrated to be a reliable reference RNA-expressed control because of its stability and constant expression in Salmo salar organs, in both the presence and the absence of the ISA virus. (thefishsite.com)
In addition to molecular testing for the etiological diagnosis of COVID-19, which is mainly based on the detection of viral RNA using reverse transcriptase polymerase chain reaction techniques (rRT-PCR) on nasopharyngeal swabs, laboratory diagnostics may measure specific IgM, IgG and IgA classes antibodies against SARS-CoV-2 antigens. (medrxiv.org)
The QIAprep& Viral RNA UM Kit and QIAprep& Viral RNA QIAsymphony Kit are intended for molecular biology applications. (qiagen.com)
The QIAprep& Viral RNA UM Kit is an innovative liquid-based approach to viral RNA epidemiology. (qiagen.com)
The QIAprep& Viral RNA QIAsymphony Kit can be used for automated sample preparation and assay set up on the QIAsymphony SP/AS. (qiagen.com)
The QIAprep& Viral RNA QIAsymphony Kit requires the installation of a custom protocol available from QIAGEN Services. (qiagen.com)
The QIAprep& Viral RNA UM Kit can be used in conjunction with the SARS-CoV-2 N1+N2 Assay Kit . (qiagen.com)
Want to try the QIAprep& Viral RNA UM Kit for the first time? (qiagen.com)
The assay performance in terms of specificity and sensitivity has been verified using the QIAprep& Viral RNA UM Kit. (qiagen.com)
The end-to-end liquid-based workflow of the QIAprep& Viral RNA UM Kit takes under one hour from start to finish. (qiagen.com)
By tracing innate immune response cell signalling, the scientists established the RNA m6A methylation modification prevents the host immune system from recognising the difference between viral and host RNA. (drugtargetreview.com)

Bioinformatics analysis and luciferase reporter assay were used to validate interactions among hsa_circ_0046060, miR-338-3p, and G6PC2. (hindawi.com)
The results of luciferase reporter assay revealed that miR-338-3p and G6PC2 were the potential targets of has_circ_0046060. (hindawi.com)

This was related to an increase in TF messenger RNA (mRNA) stability. (imperial.ac.uk)
Mechanistically, 3‑hydroxy‑3‑methylglutaryl‑CoA synthase 1 (HMGCS1) was identified as one of the direct downstream targets of VIM‑AS1, and VIM‑AS1 promoted HMGCS1 expression by enhancing HMGCS1 mRNA stability through a VIM‑AS1/insulin like growth factor 2 mRNA binding protein 2 (IGF2BP2)/HMGCS1 RNA‑protein complex. (spandidos-publications.com)
The Ccr4-Not complex removes mRNA poly(A) tails to regulate eukaryotic mRNA stability and translation. (elifesciences.org)
Consequently, motif quality is a major determinant of mRNA stability for Puf3 targets in vivo and can be used for the prediction of mRNA targets. (elifesciences.org)

The intracellular glucose intake and glycogen content were measured using a High Sensitivity Glucose Assay Kit and Glycogen Assay Kit, respectively. (hindawi.com)
Using the standard isotopic protocol, secretion of hGH protein into the culture-medium supernatant can be monitored approximately 24hours post-transfection, depending on the cell type and the sensitivity of the assay used. (thomassci.com)
Assay design is also highly variable and can make the difference between PCR success and failure and also contributes to the reproducibility and sensitivity of an assay. (sigmaaldrich.com)
The kit is highly selective for dsDNA over RNA, and offers advantages in stability, linear dynamic range, and sensitivity over other traditional methods of DNA quantitation. (mobitec.com)
In parallel, further development and validation studies were conducted according to CLSI guidelines (Analytical Validation of OncoMasTR according to CLSI Guidelines: Precision, Linearity, Sensitivity, RNA Input, Interference, Kit Stability. (cepheid.com)

RNA Interference (RNAi) technology has made it possible to effectively regulate gene expression. (altogenlabs.com)

These two VA RNA genes are distinct genes in the adenovirus genome. (wikipedia.org)
Our meta-analysis suggests a widespread role for TEs in shaping RNA-protein regulatory networks in the human genome. (biomedcentral.com)
Zika virus (ZIKV), a mosquito-borne transplacentally transmissible flavivirus, is an enveloped virus with an ~10.8 kb plus-strand RNA genome that can cause neurological disease. (mdpi.com)
The Streptococcus pyogenes Cas9 (SpCas9) nuclease can be efficiently targeted to genomic loci by means of singleguide RNAs (sgRNAs) to enable genome editing 1 - 10 . (cdc.gov)

TUNEL assays indicated that cellular fluorescence intensity declined with the rising doses of Res. (bvsalud.org)

Pellicular anion-exchange chromatography applied to RNAi assays for monitoring strand stoichiometry and RNA stability. (chromatographytoday.com)
RNAi products require both single strand and duplex impurity assays. (chromatographytoday.com)
These introduce diastereoisomers that may impact the RNAi stability or efficacy. (chromatographytoday.com)
The RNAi approach conscripts a subset of microRNA (miRNA) processing machinery, allowing exogenously supplied RNA of specific sequence to stimulate turnover of accordingly-specific mRNA [6]. (chromatographytoday.com)
There are two copies of this RNA called VAI or VA RNAI and VAII or VA RNAII. (wikipedia.org)

RNA-binding proteins contribute to specificity by interacting with both Ccr4-Not and target mRNAs, but this is not fully understood. (elifesciences.org)
the CRISPR-associated nuclease Cas9 (SpCas9), a specificity-determining CRISPR RNA (crRNA), and an auxiliary trans-activating crRNA (tracrRNA) 11 . (cdc.gov)

Serological assays are laboratory-based (both enzyme-linked adsorbent-ELISA- and Chemiluminescent-CLIA-immunoassays), point-of-care (POCT) tests recognizing the nucleocapsid (N), spike protein (S) and its S1/S2 subproteins or the receptor binding domain (RBD) with different analytical and clinical performances. (medrxiv.org)
Two clones were selected to evaluate the test performance of three assays to measure antibodies to Pgp3: multiplex bead assay (MBA), enzyme-linked immunosorbent assay (ELISA), and lateral flow assay (LFA). (cdc.gov)

Total RNA was extracted and probed for IL-2 mRNA by ribonuclease protection assay. (biomedcentral.com)

The assay exhibits high reproducibility and precision and works well in 384-well plates and in 5 μl to 20 μl samples. (illinois.edu)

Circular RNA (circRNA) sequencing was performed on five pairs of BCa samples, and circFAM13B (hsa_circ_0001535) was screened out because of its remarkably low expression in BCa. (biomedcentral.com)

C1QBP knockdown and overexpression cells were established via lentiviral infection and subjected to apoptosis and ROS assay in vitro . (medsci.org)
Polymerase chain reaction (PCR) is a powerful core molecular biology technique that is an efficient and rapid in vitro method for enzymatic amplification of specific DNA or RNA sequences from various sources. (sigmaaldrich.com)
In vitro analyses suggest that the stability of the doublet complex and single AD complex are different. (psu.edu)

Since 2001, LabNet reference laboratories in Australia, Cote d'Ivoire, Netherlands, Turkey, Uganda, the UK, and the United States have been working to 1) determine IgM and RNA stability in DBS and OF samples and 2) optimize the methods for IgM antibody assay and protocols for RNA detection in DBS and OF samples ( 8--10 ). (cdc.gov)
The expression stability of the ten genes was analyzed using four methods (geNorm, NormFinder, BestKeeper, and ∆Ct). (biomedcentral.com)
Third, to assist in the diagnosis of patients who present later in their disease course or when the standard RNA detection methods are negative but clinical suspicion is high and other diseases have been ruled out [ 6 , 7 ]. (medrxiv.org)
Quantitative studies of RNA-protein interactions are quite cumbersome using traditional methods. (illinois.edu)
MATERIALS AND METHODS: The toxicity of ATP on HaCaT cells was examined by the MTT assay. (bvsalud.org)
Using the SURVEYOR nuclease assay 13 , we assessed the ability of each Cas9-sgRNA complex to generate indels in human embryonic kidney (HEK) 293FT cells through the induction of DNA doublestranded breaks (DSBs) and subsequent nonhomologous end joining (NHEJ) DNA damage repair (Online Methods). (cdc.gov)

VAI RNA functions as a decoy RNA for the double stranded RNA activated protein kinase R which would otherwise phosphorylate eukaryotic initiation factor 2. (wikipedia.org)
The hGH assay system differs from most of the other commonly used reporter proteins (CAT, β-Gal, or luciferase) in one important respect: hGH is a secreted protein, and is measured using samples of culture-medium supernatants thus (i) avoiding the necessity to lyse cells, (ii) allowing continuous monitoring of transient expression kinetics, and (iii) allowing use of the cells for other purposes (e.g. (thomassci.com)
We analyzed binding of RNA targets by vigilin/DDP1/SCP160p and by c-myc coding region instability determinant (CRD) binding protein, CRD-BP. (illinois.edu)
We analyzed suitability of the microplate-based FA assay for high-throughput screening for small-molecule regulators of RNA-protein interactions. (illinois.edu)
To demonstrate the potential of this assay for screening libraries of small molecules to identify novel regulators of RNA-protein interactions, we identified neomycin and H33342 as inhibitors of binding of vigilin to the vitellogenin mRNA 3′-UTR. (illinois.edu)
When a cell needs to make a particular protein, it first copies the instructions from the matching gene into a molecule known as a messenger RNA (or an mRNA for short). (elifesciences.org)
Here, we study a collection of 75 CLIP-Seq experiments mapping the RNA binding sites for a diverse set of 51 human proteins to explore the role of TEs in post-transcriptional regulation of human mRNAs and lncRNAs via RNA-protein interactions. (biomedcentral.com)
It involves crosslinking cells to lock RNA-protein interactions, immunoprecipitating the complexes using an antibody specifically targeted to the RBP, sequencing cDNA reverse transcribed from the captured RNA, and statistically analyzing the aligned sequencing reads [ 26 ]. (biomedcentral.com)
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. (rndsystems.com)

Transient transfection assays have shown that VAI-RNA increases the stability of ribosome-bound transcripts. (wikipedia.org)

The expression levels of RNA of hsa_circ_0046060, mmu_circ_0002819, and miR-338-3p were determined by quantitative real-time polymerase chain reaction (RT-qPCR). (hindawi.com)
Rao S, Procko E, Shannon MF: Chromatin remodeling, measured by a novel real-time polymerase chain reaction assay, across the proximal promoter region of the IL-2 gene. (biomedcentral.com)
RNA sequencing versus real-time polymerase chain reaction versus microarrays, as well as miRNA profiling versus selected miRNA panels) and other methodological differences ( e.g. , characteristics and size of patient and control groups, sampling time, or use of plasma or serum). (medscape.com)

The reaction mixture is heated to 37 ˚C, which allows for the production of complementary cDNA copy from the RNA sample by reverse transcriptase. (sigmaaldrich.com)

Reference genes are used for the normalization of gene expression because of the stability of expression levels among different tissues, different developmental stages, or under various treatments [ 5 ]. (biomedcentral.com)
With the increasing application of high-throughput sequencing, RNA-seq has provided a new strategy for identifying new highly stable reference genes from transcriptome data. (biomedcentral.com)
Since RNA represents the transcription of genes and can therefore only be produced by living organisms, it provides a marker to determine if an organism is alive. (ubc.ca)
The assays were designed to target genes that are essential to vital processes, then used to assess their presence and abundance through real-time reverse transcription polymerase chain reaction (PCR). (ubc.ca)

We identify an important role for DDX5 in G 1 -S-phase progression where it directly regulates DNA replication factor expression by promoting the recruitment of RNA polymerase II to E2F-regulated gene promoters. (aacrjournals.org)
We examined the effects of SCD inhibition on Wnt signaling, including the expression and stability of low-density lipoprotein-receptor-related proteins 5 and 6 (LRP5 and LRP6), by immunoblot and quantitative polymerase chain reaction analyses. (nih.gov)

VAI RNA is processed in the cell to create 22 nucleotide long RNAs that can act as siRNA or miRNA. (wikipedia.org)

We performed northern blot analyses for the guide RNA truncations and found increased levels of expression for the longer tracrRNA sequences, suggesting that improved target cleavage was at least partially due to higher sgRNA expression or stability ( Fig. 1c ). (cdc.gov)

In the study published in Nature Microbiology , researchers demonstrated that HMPV hides from the innate immune system by modifying its RNA with an N6-methyladenosine modification often seen in human RNA. (drugtargetreview.com)

This work by Dagoberto Seplveda et al, University Catlica de Valparaso, Chile, aims to design a unique RT-qPCR assay for pathogen detection in the three salmonid species reared in Chile. (thefishsite.com)
we would then be able to design a transversal RT-qPCR assay that would allow us to use the same parameters to accurately determine the quantity and quality of the samples, regardless of the salmonid species tested and thereby reliably diagnose RNA pathogens. (thefishsite.com)
Because salmon production in Chile relies on two additional species that are different from Salmo salar , we decided to compare the potential nucleotide variability in the RT-qPCR amplifiable target region of ELF1α in all three salmonid fish species reared in Chile, as variability in this region could be causing the species-dependent performance of the assay. (thefishsite.com)
For labs that need to track currently relevant mutations of the virus, wet-lab tested LNA-based qPCR genotyping assays have been designed to detect the variants of concern (VOCs). (qiagen.com)

We detected SARS-CoV-2 RNA by quantitative reverse transcription PCR ( 6 ). (cdc.gov)
RNA stability assay was applied to characterize the mechanism of C1QBP regulating XDH transcription. (medsci.org)
and we have recently begun to study effects of chronic TNF on chromatin remodelling across the IL-2 proximal promoter (pIL-2), which precedes initiation of transcription, using the chromatin accessibility real-time PCR (CHART-PCR) assay [ 3 ]. (biomedcentral.com)

A more stable IRE/IRP complex in the AD brain could increase stability of the transferrin receptor mRNA and inhibit ferritin synthesis. (psu.edu)

Results Precision was below or equal to 4% for both IgM and IgG, in all the studied levels, whilst a slightly significant deviation from linearity was observed for both assays in the range of values covering the manufacturer's cut-off. (medrxiv.org)
Conclusions Analytical imprecision is satisfactory as well as the linearity, particularly when taking into account the fact that both assays are claimed to be qualitative. (medrxiv.org)

We detect widespread interactions between RNA binding proteins (RBPs) and many families of TE-derived sequence in the CLIP-Seq data. (biomedcentral.com)

Using a two-color assay to simultaneously monitor poly(A) tail removal from different RNAs, we demonstrate that Puf3 can distinguish between RNAs of very similar sequence. (elifesciences.org)
We find that SpCas9 tolerates mismatches between guide RNA and target DNA at different positions in a sequence-dependent manner, sensitive to the number, position and distribution of mismatches. (cdc.gov)

Development of molecular assays to detect the presence. (ubc.ca)
RNA-based molecular diagnostic assays were developed to detect the presence of living fungi and fungi-like organisms of phytosanitary concern. (ubc.ca)
Confidently detect more with Archer NGS assay solutions for your solid tumor, blood cancer, immune profiling, and genetic disease research. (idtdna.com)

The focus of our project is to create synthetic aptamers using specialized DNA or RNA bases to act as competitive inhibitors for PLD1 allowing patients to continue chemotherapy. (asdrp.org)

The iQuant™ dsDNA HS Assay Kit is designed specifically for an easy and accurate quantitation of dsDNA. (mobitec.com)

CircRNAs are more resistant to degradation compared with mRNAs and exhibit high biological stability because of the complete circular covalently linked structure [ 7 , 8 ]. (hindawi.com)
A microRNA is a small non-coding RNA molecule, which functions in the post-transcriptional regulation of gene expression by partially complementing with the 3′-untranslated regions (3'UTR) of specific mRNAs [ 14 ]. (biomedcentral.com)

Additionally, hGH is a mammalian gene that may contribute to the apparent high stability of the hGH mRNA in most mammalian cells. (thomassci.com)

In this report we demonstrate that in normal human brain extracts, the IRP is detected as a double IRE/IRP complex by RNA band shift assay, but in 2 of 6 Alzheimer's brain (AD) extracts examined a single IRE/IRP complex was obtained. (psu.edu)
Western blot and RNA band super shift assay demonstrate that IRP1 is involved in the formation of the single IRE/IRP complex. (psu.edu)

In vivo , orthotopic tumor xenografts assay was performed to investigate the role of C1QBP in RCC progression. (medsci.org)

Overall, each assay demonstrated high accuracy and precision when tested using either clone, and the clones were stable when stored at -20C and 4C for almost 2years. (cdc.gov)

Here, we reconstitute accelerated and selective deadenylation of RNAs containing AU-rich elements (AREs) and Pumilio-response elements (PREs). (elifesciences.org)

The biocentric generic human immunodeficiency virus (HIV) load test was used to quantify a ribonucleic acid (RNA) HIV-1. (scirp.org)

Finally, we profiled biopsies from patients with relapsed/refractory DLBCL enrolled in the fostamatinib phase IIb clinical trial using three independent RNA expression platforms: NanoString, Affymetrix, and qNPA. (aacrjournals.org)

The results illustrated that for treated samples, DNA remained stable over a period of 10 days post treatment, whereas RNA could not be detected after 24 hours for Phytophthora ramorum or 96 hours for Grosmannia clavigera. (ubc.ca)
RNA is inherently less stable than DNA due to its chemical structure. (idtdna.com)

Trends toward more conservative management of papillary thyroid cancer (PTC) diminish the primacy of serum thyroglobulin (Tg) assays as a posttreatment surveillance tool. (medscape.com)
miR-146a-5p and miR-221-3p hold remarkable promise as serum biomarkers for post-treatment monitoring of PTC patients, especially when Tg assay results are uninformative. (medscape.com)

Nevertheless, massive application of this technique requires correct setup of the assay, including the necessary controls, for unequivocal interpretation of the results. (thefishsite.com)
Moreover, results from bulk transcriptome sequencing (RNA-seq) and SCT experiments revealed distinct transcriptomic signatures representing cell injury and stress in primary RPTE in contrast to the uncultured, freshly dissociated PT from human kidney. (simonsfoundation.org)

Although macrophage TF expression is known to be regulated at the RNA level, very little is known about the mechanisms involved. (imperial.ac.uk)
Rescue assays indicated that knockdown of HMGCS1 expression ameliorated the increase in proliferation and enzalutamide resistance of prostate cancer cells induced by VIM‑AS1 overexpression. (spandidos-publications.com)
However, the initial rate of decay was similar in control and TNF-treated cells, suggesting that reduced expression in TNF-treated cells was not due to decreased stability of IL-2 mRNA. (biomedcentral.com)
IRPs regulate expression of ferritin and transferrin receptor at the mRNA level by interacting with a conserved RNA structure termed the iron-responsive element (IRE). (psu.edu)
The tissue-specific expression patterns of these RNA species and their high stability in biological fluids are well established. (medscape.com)

Yang G, Wu Y, Wan R, Sang H, Liu H and Huang W: The role of noncoding RNAs in the regulation, diagnosis, prognosis and treatment of osteosarcoma (Review). (spandidos-publications.com)

Studies report that circular RNAs (circRNAs) derived from exosomes may play a key role in GDM development. (hindawi.com)
As even the slightest exposure to RNase can impact RNA stability, IDT has not performed rigorous long term stability studies for RNA. (idtdna.com)

On the other hand we also assay the nutrients such as glucose, ammonia, phosphorus and protease during the fermentation. (ukessays.com)

however, the strength of the recommendation to treat should be based on the patient's willingness to accept therapy as well as the prognosis for AIDS-free survival as determined by the HIV RNA copy per mL of plasma and the CD4+ T cell count. (cdc.gov)
again, risk of progression to AIDS, as determined by HIV RNA viremia and CD4+ T cell count, should guide the decision to treat. (cdc.gov)
The functions of circFAM13B were verified by proliferation assays, glycolysis assays, BCa cells-CD8 + T cell co-culture assays and tumorigenesis experiment among human immune reconstitution NOG mice. (biomedcentral.com)
Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. (rndsystems.com)

High-throughput RNA sequencing and bioinformatics analysis have led to the identification of several novel circRNAs that exhibit biological characteristics and regulatory functions [ 6 , 9 ]. (hindawi.com)

High-resolution computed tomography (HRCT) of virus 2 (SARS-CoV-2) RNA on disposable wooden chop- her lungs revealed small consolidations and ground- sticks used by 5 consecutive asymptomatic and posts- glass opacities in both lower lobes, left upper lobe, ymptomatic patients admitted for isolation and care at our and right middle lobe. (cdc.gov)

Although an ELF1α-driven assay has been successfully used for ISAV detection in Salmo salar samples, it is also known to have a highly variable performance when used for the diagnosis of other reared salmonid species, particularly Oncorhynchus kisutch and Oncorhynchus mykiss , which together with Salmo salar , constitute the economically important species cultivated in Chile. (thefishsite.com)

Exosomes secreted by the umbilical cord mesenchymal stromal cells (UMSCs) and circular RNAs (circRNAs) derived from exosomes have been shown to be associated with the progression of GDM-related complications. (hindawi.com)
Chronic culture of murine T cells in TNF does not alter the stability of IL-2 mRNA induced via TCR, or by PMA and ionomycin, in those cells. (biomedcentral.com)
We applied an assay that measures the stability of maintenance of an episomal plasmid in human tissue culture cells to screen for new DNA replication factors. (aacrjournals.org)
Reprogramming triggers a cascade of evident changes in the host cells that are recognizable morphologically and through a combination of markers and pluripotency assays. (cellapplications.com)

Research has shown that human metapneumovirus uses RNA methylation to hide from the immune system and that knocking out this methylation creates a mutant strain which acts like a vaccine. (drugtargetreview.com)

Anatomy5

Organisms5

Diseases1

Chemicals and Drugs61

Analytical, Diagnostic and Therapeutic Techniques and Equipment18

Phenomena and Processes50

Information Science3

Health Care2

Immunoprecipitation4

Viral18

Luciferase reporter assay2

MRNA stability4

Sensitivity5

Interference1

Genome4

Fluorescence1

RNAi5

Specificity2

ELISA2

Total RNA1

Reproducibility1

Circular RNA1

Vitro3

Methods6

Protein9

Transfection1

Real-time pol3

CDNA1

Genes4

Polymerase2

MiRNA1

Sequences1

Microbiology1

QPCR4

Transcription3

Synthesis1

Linearity2

Proteins1

Sequence2

Detect3

Aptamers1

Quantitation1

MRNAs2

Gene1

Demonstrate2

Tumor1

Precision1

Selective1

Quantify1

Clinical1

Stable2

Serum2

Results2

Expression5

Regulation1

Studies2

Glucose1

Cell4

Regulatory1

High1

Species1

Cells4

Immune1