Assay protein binding. Medical search. Frequent questions

Selective microfluidics-based ligand enrichment followed by sequencing, or SMiLE-seq, combines antibody arrays, mechanical trapping, and next-generation sequencing readouts to provide a new platform for characterizing DNA-protein interactions. (genomeweb.com)
By physically restraining bound molecules against an antibody array, they capture both transient interactions, which yeast two-hybrid or tandem mass spectrometry methods don't, and weak interactions, which are usually obliterated by the wash in protein-DNA microarrays. (genomeweb.com)
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human AE Binding Protein 1 (AEBP1) in tissue homogenates, cell lysates and other biological fluids. (kits-elisa.com)
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human AE Binding Protein 1 (AEBP1) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (kits-elisa.com)
Intraclass correlation coefficients based on blinded replicate sample testing, reflecting the proportion of total variance that is between-sample rather than within-sample variability of severe acute respiratory syndrome coronavirus 2 antibody detection in study of commercially available high-throughput assays for serosurveillance. (cdc.gov)
Standards and samples are pipetted into the wells and anyXBP1 present is bound by the immobilized antibody. (abbkine.com)
Based on Bio-Layer Interferometry (BLI), Octet ® BLI systems enable real-time, label-free analysis for the determination of kinetics, affinity and antibody/protein quantitation. (sartorius.com)
Antibody responses against spike protein were high in children and seroconversion boosted responses against seasonal Beta-coronaviruses through cross-recognition of the S2 domain. (nature.com)
After the addition of biotinylated T3 and streptavidin-coated microparticles, the still-free binding sites of the labeled antibody become occupied, with formation of an antibody-hapten complex. (cdc.gov)
Dilute the Capture Antibody to the working concentration in PBS without carrier protein. (rndsystems.com)
All the 88 samples that were analyzed for neutralizing antibodies and antibody avidity revealed a rapid decay of neutralizing and binding IgG antibodies against SARS-CoV-2 and low avidity during the 181 days of follow-up. (news-medical.net)
Serial testing of anti-spike protein antibody binding and neutralizing antibody titers in serum collected from 233 long-stay nursing home residents from the time of their primary vaccination series and including any subsequent booster doses, including the bivalent vaccine, was performed. (cdc.gov)
[ 1 , 2 ] PEG, a water-soluble linear polymer, appears to accelerate antibody-RBC binding by steric exclusion of water molecules in the diluents and to promote antibody detection. (medscape.com)
AHG is an animal antibody that binds to the F c portion of human immunoglobulin. (medscape.com)
The AHG detects bound RBC antibodies that do not produce direct agglutination (sensitizing antibody). (medscape.com)
The presence of agglutination with the addition of AHG indicates antibody binding to a specific red cell antigen. (medscape.com)
The indirect fluorescent antibody (IFA) assay is the diagnostic test of choice for detection of B. duncani specific antibodies. (cdc.gov)
Antibody binding to bacterial surfaces plays a crucial role in immunity, and a key characteristic of this protein-protein interaction is the binding affinity. (lu.se)
Determining the affinity of an antibody binding to its antigen is the first step in predicting the function in a physiological environment where other competing protein interactions may be present. (lu.se)
Antibody-antigen affinity is often evaluated with isolated proteins. (lu.se)
It is informative to also be able to assess antibody binding to a bacterial surface where many antigens might be present, including multiple copies of the specific antigen the antibody recognizes, and in a context where the antigen might be in a more natural conformation. (lu.se)
In this chapter, we present a flow cytometry-based assay to measure and calculate the cell surface binding affinity or avidity of any mono- or polyclonal antibody solution. (lu.se)
Kumra Ahnlide, V & Nordenfelt, P 2023, Measurement of Antibody Binding Affinity on Bacterial Surfaces Using Flow Cytometry . (lu.se)

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. (assayspro.com)
With a wide range of ligand capture surfaces that allow for immobilization of antibodies, recombinant proteins, DNA, RNA and virus-like particles, the Octet ® BLI platform has become a must-have laboratory tool for biomolecular interactions studies. (sartorius.com)
The sample is incubated with biotinylated Tg and the antibodies of the sample bind the antigen. (cdc.gov)
After the addition of anti-Tg antibodies labeled with ruthenium complex and streptavidin-coated microparticles, the immunocomplex produced becomes bound to the solid phase via interaction of biotin and streptavidin. (cdc.gov)
Assays for anti-thyroid peroxidase (anti-TPO) and antithyroglobulin (anti-Tg) antibodies may be helpful in determining the etiology of hypothyroidism or in predicting future hypothyroidism. (medscape.com)
The specific target for this project is a set of more sensitive assays for influenza, through a combination of chemical amplification and replacing antibodies by novel binding proteins being developed by the David Baker Lab. (washington.edu)
The seropositive samples were then subjected to chemiluminescent microparticle immunoassay to detect anti-receptor binding domain (RBD) IgG antibodies. (news-medical.net)
Neutralizing antibodies were detected using the surrogate virus neutralization test (sVNT) assay, which mimics the interaction between the virus and the host using an immobilized angiotensin-converting enzyme-2 (ACE-2) receptor and purified RBD proteins. (news-medical.net)
Specific antibodies, if present, bind to the 21-hydroxylase during this time. (medscape.com)
Several proteins were identified by electrospray ionization (ESI) mass spectrometric analysis and four recombinant protein constructs were expressed and used in a multiplex bead assay (MBA) to detect B. duncani-specific antibodies. (cdc.gov)

Changes in emission spectra of tryptophan are due to the protein conformational transitions, subunit association, ligand binding or denaturation, which affect the local environment surrounding the indole ring. (cgiar.org)
Reduction in the intensity of tryptophan emission spectra of mitochondrial membrane proteins in presence of an increasing concentration of a ligand can be used to study the interaction of insecticides/drugs with the intact mitochondria. (cgiar.org)
Furthermore, this assay can be readily adapted for studying protein-ligand interactions in intact mitochondria and in other cell organelles extending its implications for pesticide and pharma industry and in drug discovery. (cgiar.org)
Our Surface Plasmon Resonance (SPR) Biacore T100 biosensor instrument is a high-throughput equipment that allows the study of protein-protein and protein-ligand kinetics and thermodynamics. (utmb.edu)
The kinetic of elevation of intracellular [Ca 2+ ] slowed with increasing fatty acid chain length, suggesting different ligand on-rates, whereas the addition of fatty acid-free bovine serum albumin reduced signals, presumably by binding the fatty acids. (aspetjournals.org)
To allow effective ligand equilibration, GPR40-Gα q was used in guanosine 5′- O -(3-[ 35 S]thio)triphosphate ([ 35 S]GTPγS) binding assays. (aspetjournals.org)
EphA5, also known as Ehk1, Bsk, Cek7, Hek7, and Rek7 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. (rndsystems.com)
Activation of kinase activity occurs after ligand recognition and binding. (rndsystems.com)
Clinically approved AR antagonists compete with dihydrotestosterone (DHT) for binding to the ligand-binding domain (LBD) of AR, and patients eventually develop resistance to these treatments. (aacrjournals.org)
Frankfurt am Main, Germany about half of the crystallographically visible water molecules appear to be expelled by the ligand, we find that ligand binding actually increases the number of water molecules within the cavity. (lu.se)

Protein S activity can also be measured, but the assay is technically difficult and is associated with a high false-positive rate (10 to 15%) so more reproducible antigen assays are favored. (msdmanuals.com)

We demonstrated that this cell line responds to compounds typically used in the field of sGC research and that it represents an innovative and robust assay to screen for sGC modulators with high efficiency and high sensitivity by means of standard luminescence readers. (nih.gov)
The sensitivity of the established dual RAP assay was 2.4-2.8 copies/reaction, with higher reproducibility and specificity . (bvsalud.org)
Rat X-box-binding protein 1 (XBP1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat XBP1. (abbkine.com)
The particle-enhanced reaction determines a very good sensitivity for the assays allowing the measurement of the extremely low urinary levels and results in excellent method performance. (clinlabint.com)
Temperature sensitivity of processing of viral proteins. (microbiologyresearch.org)
To significantly improve data quality and sensitivity in biochemical assays. (fishersci.com)

Bacteria often can't support over-expression needed to get workable quantities of protein for an assay, and in vitro expression often can't synthesize a fully functional transcription factor. (genomeweb.com)
Plasma protein binding is one of Cyprotex's in vitro ADME screening services. (cyprotex.com)
To determine how the ASAP complex interacts with the EJC core, we performed in vitro coprecipitation assays 3 with Tandem Affinity Purification (TAP)-tagged recombinant proteins corresponding to full-length human SAP18, RNPS1 and ACINUS. (nature.com)
Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro . (rndsystems.com)
For the end-point of skin sensitization, the ultimate goal, i.e. the development and validation of alternative in vitro/in silico assays by 2013, may be achieved through a better understanding of the skin sensitization process on the cellular and molecular levels. (cdc.gov)
Thus, the principle of protein or peptide haptenation could be used in in vitro assays to predict the sensitization potential of a new chemical entity. (cdc.gov)
Once sensitized local lymph node assay (LLNA) (6), as no vali- to that chemical, the person is susceptible to dated in vitro alternatives exist to date (7). (cdc.gov)
It is likely that should lead to useful insights for the development improving our understanding of the cellular and of in vitro assays (17, 18). (cdc.gov)

k) and the local lymph node assay threshold (EC3) values. (cdc.gov)

This data analysis calculates concentrations using a Four Parameter Logistic (4PL) curve fit in accordance with Arbor Assays' Retinol Binding Protein EIA Kit (K062-H1, K062-H5). (myassays.com)
TRIMERO Diagnostics offers IVD CE marked Retinol Binding Protein assays, for serum and urine samples, available for nephelometers and the most popular Clinical Chemistry analyzers present on the market. (clinlabint.com)
Combined measurement of ferritin, soluble transferrin receptor, retinol binding protein, and C-reactive protein by an inexpensive, sensitive, and simple sandwich enzyme-linked immunosorbent assay technique. (who.int)

Analysis of binding kinetics reveals that this is primarily due to differences in dissociation rate constants. (elifesciences.org)
A microfluidic-free design allows for unprecedented time and cost savings for binding kinetics, affinity and analyte concentration measurement with high precision, accuracy and a broad dynamic range. (sartorius.com)
From AnaSpec: Truncated fluorescently labeled amyloid-beta peptide containing metal binding residues to study its metal interaction characteristics and kinetics. (anaspec.com)
When enzymes bind multiple substrates, such as dihydrofolate reductase (shown right), enzyme kinetics can also show the sequence in which these substrates bind and the sequence in which products are released. (wikipedia.org)
These findings may have significant implications for the strength, specificity and kinetics of lipid binding. (lu.se)

Description: A sandwich quantitative ELISA assay kit for detection of Human AE Binding Protein 1 (AEBP1) in samples from tissue homogenates, cell lysates or other biological fluids. (kits-elisa.com)
Description: A sandwich ELISA kit for detection of AE Binding Protein 1 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (kits-elisa.com)
To establish a nested recombinant enzyme -assisted polymerase chain reaction (RAP) technique combined with recombined mannose-binding lectin protein (M1 protein )- magnetic beads enrichment for the detection of Candida albicans (C. albicans) and Candida tropicalis (C. tropicalis) in blood samples for the early diagnosis of candidemia albicans and candidiemia tropicalis. (bvsalud.org)
In this study, a dual RAP assay for the detections of C. albicans and C. tropicalis in blood sample was developed, which has the advantages of accuracy, rapidity, and less contaminants and has great potential for rapid detection of Candidemia . (bvsalud.org)
Development of an Analytical Assay for Electrochemical Detection and Quantification of Protein-Bound 3-Nitrotyrosine in Biological. (aidstar-one.com)
Enzyme-linked immunosorbent assay (ELISA) was used for the initial detection of the viral spike protein . (news-medical.net)

A peptide can be labeled with a single or multiple dye(s) at the peptide N-terminus, C-terminus, or internally, for applications in protein interaction studies , to trace the presence of biomolecules in cells, enzyme activity assays , etc. (anaspec.com)
pH-and Temperature-Dependent Peptide Binding to the Lactococcus lactis Oligopeptide-Binding Protein A Measured with a Fluorescence Anisotropy Assay. (anaspec.com)
A cell-penetrant peptide blocking C9ORF72-repeat RNA nuclear export reduces the neurotoxic effects of dipeptide repeat proteins. (le.ac.uk)
Biochemical and functional evidence suggest that the calcitonin receptor-like receptor (CRLR) interacts with receptor activity-modifying protein-1 (RAMP1) to generate a calcitonin gene-related peptide (CGRP) receptor. (acs.org)

GC exists in two isoenzyme forms: soluble and membrane-bound. (nih.gov)
While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). (rndsystems.com)

XCL1 induces it chemotactic function by binding to a chemokine receptor called XCR1. (abbkine.com)
The current studies provide novel insights into the pharmacology of GPR40 and indicate that G protein-coupled receptors which respond to fatty acids, and potentially to other lipid ligands, can be occupied by endogenous agonists before assay and that this may mask the pharmacology of the receptor and may be mistaken for high levels of constitutive activity. (aspetjournals.org)
These observations presented a spatial and temporal resolution of the sequence of events underlying receptor-evoked involvement of tubulin in G-protein-mediated signaling. (jneurosci.org)
In addition to shed new light on the CRLR−RAMP1 signaling complex, the BRET assays developed herein offer new biosensors for probing CGRP receptor activity. (acs.org)

Do not miss to order the product Human Mannma binding protein/mannan binding lectin,MBP/MBL ELISA Kit at this promotional price until the end of December 2017. (assayspro.com)
After delivery, the specialists from Gentaur/Genprice recommend you to store the Human Mannma binding protein/mannan binding lectin,MBP/MBL ELISA Kit between two and eight degrees Celsius to keep the quality and activity of the reagents included in the kit. (assayspro.com)
This Rat X-box-binding protein 1 (XBP1) ELISA Kit employs a two-site sandwich ELISA to quantitate XBP1 in samples. (abbkine.com)
Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples. (abbkine.com)

The most important physiological activator of sGC is nitric oxide, which activates the enzyme upon binding to the heme moiety. (nih.gov)
In order to find new modulators for this important enzyme, an innovative cell-based assay has been developed and optimized for the use in high-throughput screening. (nih.gov)
Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of XBP1 bound in the initial step. (abbkine.com)
The Octet ® BLI platform also provides robust monitoring of biomolecular interactions to overcome limitations in throughput, performance and cost associated with traditional technologies such as enzyme-linked immunosorbent assay (ELISA) or high-performance liquid chromatography (HPLC). (sartorius.com)
in such cases, it is helpful to determine the enzyme structure with and without bound substrate analogues that do not undergo the enzymatic reaction. (wikipedia.org)
Enzyme-linked immunosorbent assay (ELISA) was used to test sera from 190 individuals. (who.int)

Plasma protein absorption assays and flow cytometry analysis revealed that pronounced binding of human fibrinogen is a common phenotype of human S. equi subsp. (lu.se)

Equilibrium dialysis is the most widely accepted method for assessing plasma protein binding as non specific binding effects are minimized compared with other methods such as ultrafiltration. (cyprotex.com)
Cyprotex's Plasma Protein Binding assay is performed using an equilibrium dialysis method and delivers a value of fraction of compound unbound to proteins (fu). (cyprotex.com)
Equilibrium dialysis is used to determine the extent of binding of a compound to plasma proteins. (cyprotex.com)

That year, the two published a paper in Science describing a method to trap low-affinity DNA-protein interactions, specifically those of transcription factors. (genomeweb.com)
The transcription factors bind to any number of DNA motifs in the library, those interactions are trapped, and then the readout matches up each motif with the transcription factor via the barcode. (genomeweb.com)
Deplancke also worked in advanced bioinformatics to derive predictive models for binding interactions. (genomeweb.com)
Monitor the hydrodynamic properties of biomacromolecules and protein-protein interactions. (utmb.edu)
This information can be used to analyze protein-protein interactions, the presence of oligomeric states, and conformational rearrangement of molecules. (utmb.edu)
Real-time Analysis - Binding interactions are continuously monitored as they happen. (sartorius.com)
Adenovirus protein- protein interactions: hexon and protein VI. (microbiologyresearch.org)

RNA-binding proteins contribute to specificity by interacting with both Ccr4-Not and target mRNAs, but this is not fully understood. (elifesciences.org)
Therefore, children generate robust, cross-reactive and sustained immune responses to SARS-CoV-2 with focused specificity for the spike protein. (nature.com)

The lectin-bound fraction that is used for this assay contains 7 antigenic glycoproteins. (cdc.gov)
These antigenic proteins are considered to be highly specific for cysticercosis when tested with heterologous parasitic diseases. (cdc.gov)
Diagnosis is based on antigenic assays of total or free plasma protein S (free protein S is the form unbound to the protein S carrier molecule, C4-binding protein). (msdmanuals.com)

However, treatment of membranes with fatty acid-free bovine serum albumin reduced the basal [ 35 S]GTPγS binding in a concentration-dependent manner and allowed the responsiveness and pharmacology at GPR40 of each of the fatty acids thiazolidinediones and a novel small-molecule agonist to be uncovered. (aspetjournals.org)
In pregnancy, the variation in the results of commercially available free T4 assays has led the American Thyroid Association to recommend using method-specific and trimester-specific reference ranges for serum free T4. (medscape.com)
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. (rndsystems.com)
Positive results from this assay (ie, ≥1 U/mL) indicate the presence of adrenal cortex autoantibodies in serum, a finding that is consistent with Addison disease. (medscape.com)

The recombinant plasmids were transformed into competent Escherichia coli strain BL21 and Quox1 fusion proteins were isolated by chromatographic techniques. (wikipedia.org)
As for their wild-type counterparts, fusion proteins linking CRLR and RAMP1 to the energy donor Renilla luciferase ( R luc) and energy acceptor green fluorescent protein (GFP) reach the cell surface only upon coexpression of CRLR and RAMP1. (acs.org)
Radioligand binding and cAMP production assays also confirmed that the fusion proteins retained normal functional properties. (acs.org)

However, it is important to note that the fraction unbound values for compounds which are less highly bound tend to be more variable in the 10% assay and the conversion from fu 10% to fu 100% is less accurate. (cyprotex.com)
The graph below shows the effect of the scale up from a fraction unbound at 10% and 50% to a fraction unbound at 100% for low and highly bound compounds. (cyprotex.com)

SAAB also allows the identification of sites with high relative binding affinity since the competition is inherent in the protocol. (wikipedia.org)
We can apply the technique to less affinity binding sequence also, provided to keep high concentration of binding protein at each step of binding. (wikipedia.org)
The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). (rndsystems.com)

Understand the distribution potential of your compound using our plasma protein binding assay. (cyprotex.com)
The extent of binding to plasma influences the way in which a drug distributes into tissues in the body. (cyprotex.com)
Extensive plasma protein binding also limits the amount of free compound available to access sites of action in the cell, and metabolism and elimination may be slower. (cyprotex.com)
There is a choice of three methods for assessing plasma protein binding using three different percentages of plasma to provide flexibility depending on budget and compound characteristics. (cyprotex.com)
Highly automated evaluation of plasma protein binding using a higher concentration of plasma. (cyprotex.com)
Evaluation of protein binding using 100 % plasma. (cyprotex.com)
Please provide an overview of Cyprotex's Plasma Protein Binding assay. (cyprotex.com)
In addition to using whole plasma, the plasma protein binding assay can be performed using two other ratios of plasma (10% or 50% plasma in buffer v/v). It can be difficult to detect very highly bound compounds in the protein-free compartment of the 50% and 100% assay, therefore the 10% assay is recommended if it is anticipated that the compounds are likely to be highly bound. (cyprotex.com)
The screening costs for the 10% assay are lower as less plasma is required. (cyprotex.com)
For this reason, it is recommended that the 50% plasma assay is used for less highly bound compounds. (cyprotex.com)
M1 protein - magnetic bead enriched plasma C. albicans and C. tropicalis were used for RAP and PCR in with simulated samples and the results were compared. (bvsalud.org)
M1 protein - magnetic bead enrichment of pathogen combined with the dual RAP assay could complete the detections of C. albicans and C. tropicalis in plasma within 4 hours. (bvsalud.org)
zooepidemicus strains in association with differences in M-like protein-mediated binding of host plasma proteins. (lu.se)
Protein S and protein C are, therefore, components of a natural plasma anticoagulant system. (msdmanuals.com)
Heterozygous deficiency of plasma protein S predisposes to venous thrombosis. (msdmanuals.com)
Because there is no purified protein S concentrate available for transfusion, normal plasma is used to replace protein S during a thrombotic emergency. (msdmanuals.com)

Selection and amplification binding assay (SAAB) is a molecular biology technique typically used to find the DNA binding site for proteins. (wikipedia.org)
Intrinsically disordered proteins and their contraction and folding can be monitored using this technique. (utmb.edu)
DNA FOOTPRINTING utilizes this technique to analyze the DNA contact sites of DNA-BINDING PROTEINS. (bvsalud.org)

When a cell needs to make a particular protein, it first copies the instructions from the matching gene into a molecule known as a messenger RNA (or an mRNA for short). (elifesciences.org)
The high apparent constitutive activity of GPR40-Gα q was also reversed by a small-molecule GPR40 antagonist, and basal [ 35 S]GTPγS binding was prevented by the selective Gα q /Gα 11 inhibitor YM-254890. (aspetjournals.org)
All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. (rndsystems.com)

Red blood cells (RBCs) carry numerous protein and carbohydrate antigens on their surface. (medscape.com)
Combining these two antigens did not improve the performance of the assay. (cdc.gov)

Here, using recombinant complex reconstitution and transcriptome-wide analysis, we showed that the EJC peripheral protein complexes ASAP and PSAP form distinct complexes with the EJC core and can confer to EJCs distinct alternative splicing regulatory activities. (nature.com)
However, how these complexes bind the EJC core and whether they function differently as part of EJC is still unknown. (nature.com)
The protein bound DNA was detected by autoradiography, and the bands representing protein-DNA complexes were excised from the gel and the eluted DNA were amplified by PCR using primers complementary to the 20 bp nonrandom flanking sequences. (wikipedia.org)

Western blotting was performed to detect the protein levels of TWIST and EMT-related genes. (hindawi.com)
CCK-8 and Transwell assays were performed to detect cell proliferation, invasion, and migration abilities. (hindawi.com)

Solution molecular biophysics and thermodynamic techniques are used both as a primary research tool as well as means to structural studies (for example in the exploration of conditions for crystallization of proteins). (utmb.edu)
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. (novusbio.com)
One of the key molecular events in skin sensitization is protein haptenation, i.e. the chemical modification of self-skin protein(s) thus forming macromolecular immunogens. (cdc.gov)
The basis of hapten- molecular mechanisms of the sensitization pro- protein binding work is the hypothesis that upon cess will result in novel opportunities for the skin absorption, only protein-reactive chemicals development of alternative methods for assessing (or those that can be metabolically or chemically skin sensitization hazard and relative potency of converted to protein-reactive species) are able to chemicals. (cdc.gov)

Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided. (rndsystems.com)
Dihydrofolate reductase from E. coli with its two substrates dihydrofolate (right) and NADPH (left), bound in the active site. (wikipedia.org)

however, reduced effectiveness of monovalent vaccines during the period of Omicron variant predominance led to recommendations for booster doses with bivalent COVID-19 mRNA vaccines that include an Omicron BA.4/BA.5 spike protein component to broaden immune response and improve vaccine effectiveness against circulating Omicron variants ( 2 ). (cdc.gov)

Measured by its binding ability in a functional ELISA. (rndsystems.com)

It is suggested that G-protein-coupled receptors might modulate cytoskeletal dynamics, intracellular traffic, and cellular architecture. (jneurosci.org)
Cytoskeletal proteins appear to be involved in the control of intracellular signaling. (jneurosci.org)

An example of enzymes that bind a single substrate and release multiple products are proteases , which cleave one protein substrate into two polypeptide products. (wikipedia.org)

S, spike protein. (cdc.gov)

Finally the sequence CAATC was identified as the consensus binding sequence for Quox1 homeodomain. (wikipedia.org)
By combining the power of random-sequence selection with pooled sequencing, the SAAB imprint assay makes possible simultaneous screening of a large number of binding site mutants. (wikipedia.org)
It is also possible to identify the binding site even if both the protein and sequence is not known. (wikipedia.org)
Using a two-color assay to simultaneously monitor poly(A) tail removal from different RNAs, we demonstrate that Puf3 can distinguish between RNAs of very similar sequence. (elifesciences.org)
In addition, we found evidence to indicate that conformation as well as sequence was important for good hexon binding in our assays. (microbiologyresearch.org)

1987. The L5178Y Tk +/- mouse lymphoma mutagenesis assay with API 220°-208°F distillate fraction of unleaded gasoline. (cdc.gov)

This gene encodes a mitochondrial solute carrier protein family member. (nih.gov)
These results indicate that Gc protein appears to be regulated differently than the other members of this gene family, albumin and alpha-fetoprotein (AFP), which are negative acute phase reactants. (nih.gov)

Together these proteins shorten the end of the mRNAs, preparing the molecules for degradation. (elifesciences.org)
Greiner Bio-One 384 Well Polystyrene Non-binding Flat Bottom Microplate has long-term surface stability without degradation or leaching. (fishersci.com)
Because protein S binds and assists activated protein C in the degradation of coagulation factors Va and VIIIa, deficiency of protein S predisposes to venous thrombosis. (msdmanuals.com)

IGFBPs modulate the biological activities of IGF proteins. (rndsystems.com)
However, although Michael acceptors are reactive, the Michael addition is reversible and it can be assumed that also other parts of the molecules will moderate the binding to and the release from any given nucleophilic site in a protein, and thereby moderate a specific biological activity. (lu.se)

Lyophilized from a 0.2 μm filtered solution in Glycine, Sucrose, Tween® 80 and Glutamic Acid with BSA as a carrier protein. (novusbio.com)
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. (rndsystems.com)

The product Assay kit for Canine Chromodomain-helicase-DNA-binding protein 6(CHD6) (ELISA) should be kept between two and eight degrees Celsius to ensure the retention of the stability and reactivity of the reagents included in the kit. (assaydes.com)
2010). The present study describes an amine (pyridoxylamine (PDA)) absorbance and fluorescence-based reactivity kinetic probe to complement the NBT assay for identification of amine-selective haptens. (cdc.gov)
Reaction completion time of the sensitizers also correlated strongly (R2 = 0.74) with EC3 values suggesting utility of transforming this chemical reactivity method into a simple, inexpensive end-point assay. (cdc.gov)

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. (rndsystems.com)

The entire complex becomes bound to the solid phase via interaction of biotin and streptavidin. (cdc.gov)

Techniques for measuring specific nucleic acid interaction with another nucleic acid or with a protein by digestion of the non-interacting nucleic acid by various nucleases. (bvsalud.org)

Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. (rndsystems.com)

The assay could help accelerate efforts to map where transcription factors bind the genomes of humans and model organisms, Deplancke said. (genomeweb.com)

In cells lacking expression of Ca 2+ -mobilizing G proteins, coexpression of human GPR40 and Gα q allowed medium- and long-chain fatty acids to elevate intracellular [Ca 2+ ]. (aspetjournals.org)

Free thyroid hormone levels can be estimated by calculating the percentage of available thyroid hormone-binding sites (triiodothyronine [T3] resin uptake, or thyroid hormone binding ratio [THBR]) or by measuring the TBG concentration. (medscape.com)

These results suggest that SzM-mediated binding of human fibrinogen is an important virulence mechanism of zoonotic S. equi subsp. (lu.se)
Results are independent of binding protein concentrations. (medscape.com)

This luminescent assay, which is suitable for both 96- and 384-well plate formats, has been achieved by stably expressing the alpha and beta subunits of a mutated form of sGC in Chinese hamster ovary cells. (nih.gov)

Bone morphogenetic protein 2 (BMP-2) is a member of the BMP subgroup of the TGF-beta superfamily. (novusbio.com)

The role of the mitochondrial outer membrane protein SLC25A46 in mitochondrial fission and fusion. (nih.gov)

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. (abbkine.com)
This Roche assay uses a sandwich principle. (cdc.gov)

assays are available for human FKBP12, 51 and 52. (selcia.com)
Prominent Binding of Human and Equine Fibrinogen to Streptococcus equi subsp. (lu.se)
zooepidemicus strains that the M-like protein SzM is crucial for the binding of fibrinogen to the bacterial surface and for survival in equine and human blood. (lu.se)
zooepidemicus isolates of equine and human origins were compared with regard to SzM sequences and binding of equine and human fibrinogens. (lu.se)
Furthermore, binding of human fibrinogen is associated with specific SzM types. (lu.se)
This was also observed when human embryonic kidney (HEK) 293 cells were transfected with a GPR40-Gα q fusion protein. (aspetjournals.org)
In this assay hematopoietic cells are cultured in MyeloCult™ medium with supportive human or mouse stromal cell layers and tested for their ability to generate myeloid clonogenic progenitors for several weeks as assayed by replating the cells in a colony-forming unit (CFU) assay using MethoCult™ medium. (stemcell.com)
Greiner Bio-One 96-Well, Chimney Style Non-binding Microplates with Clear™ Film Bottom are free of detectable DNase, RNase, human DNA. (fishersci.com)

ALS (Acid Labile Subunit) is a liver-derived protein that exists in a ternary complex with Insulin-like Growth Factor (IGF)-binding Protein-3 (IGFBP-3) or IGFBP-5, and either IGF-I or IGF-II. (rndsystems.com)

Isolate the DNA bound protein by EMSA: the DNA bound protein, migrated in acrylamide gel, is isolated by autoradiography as per an Electrophoretic mobility shift assay (EMSA) protocol. (wikipedia.org)

Anatomy11

Organisms13

Chemicals and Drugs111

Analytical, Diagnostic and Therapeutic Techniques and Equipment45

Phenomena and Processes77

Disciplines and Occupations2

Technology, Industry, Agriculture2

Information Science7

Health Care3

Antibody23

Antibodies10

Ligand10

Antigen1

Sensitivity6

Vitro8

Local lymph node assay1

Retinol Binding3

Kinetics5

Detection6

Peptide4

Soluble2

Receptor4

ELISA Kit4

Enzyme6

Flow cytometry1

Equilibrium Dialysis3

Interactions7

Specificity2

Antigenic3

Serum4

Fusion proteins3

Unbound2

Affinity3

Plasma16

Technique3

Molecule3

Antigens2

Complexes3

Detect2

Molecular4

Substrates2

Immune1

Functional1

Cytoskeletal2

Substrate1

Spike protein1

Sequence5

Mutagenesis1

Gene2

Degradation3

Biological2

Carrier protein2

Reactivity3

Recombinant protein1

Biotin1

Nucleic acid1

Ability to bind1

Accelerate1

Coexpression1

Uptake1

Results2

Suitable1

SuperFamily1

Mitochondrial1

Sandwich2

Human8

Subunit1

Protocol1