AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesTechnology, Industry, AgricultureHealth Care
HaptensDinitrobenzenesDermatitis, ContactAntibodies, CatalyticDinitrophenolsOxazoloneDinitrofluorobenzeneNitrobenzenesBinding Sites, AntibodyTrinitrobenzenesNitrohydroxyiodophenylacetategamma-GlobulinsAntigen-Antibody ReactionsAntibody Formationp-AzobenzenearsonateAntibodies, BispecificAntibodiesMyeloma ProteinsNitrophenolsAntibody SpecificityHemocyaninPhenylacetatesAntibody-Producing CellsHemolytic Plaque TechniqueSerum Albumin, BovineEpitopesAntigensAzo CompoundsImmunoglobulin Fab FragmentsImmunizationAntibody AffinityMice, Inbred BALB CCross ReactionsDinitrochlorobenzeneLangerhans CellsIrritantsMetalsEpidermisQuantitative Structure-Activity RelationshipPalladiumNickelAlloysSalts
AntibodiesELISAAntigenAnti-hapten antibodyReagentsSolid-phase assaySensitivityBinding AssayImmunosorbent assayDetectionMonoclonal antibodyProteinMoleculesProteinsVitroColorimetricConjugatesCellularMolecularProbesPeptideAnalyteAntigensClinicalSkipBeadMoleculeBufferBindSerumSandwichRobustDevelopmentSimplicityTestTargetInterestHumanTechnology
Antibodies12
  • Fluorescence staining and scanning of chip After incorporation of these hapten-labeled ddNTPs, multi-layered immunohistochemical assays are performed by repeated rounds of staining with a combination of antibodies to differentiate the two types. (wikipedia.org)
  • Human serum from a car painter sensitized to HDI was assayed to detect specific IgE antibodies as well as the ability of various isocyanate conjugates to inhibit radioallergosorbent tests (RAST). (cdc.gov)
  • HMI induced predominantly hapten specific antibodies whereas HDI induced antibodies specific for aliphatic isocyanate -GSA determinants. (cdc.gov)
  • Production of hapten-protein conjugates of the organophosphate pesticides chlorpyrifos and pirimiphos, and characterisation of antichlorpyrifos antibodies were also successfully performed. (dcu.ie)
  • Both the polyclonal and scFv antibodies were found to be suitable for the detection of AFBi, and the assays were reproducible. (dcu.ie)
  • Antibodies against small molecule haptens enable robust and rapid detection, monitoring and quantification using assays such as ELISA or IHC/IF. (prosci-services.com)
  • In 1971, enzyme labelled antigens and antibodies were developed as serological reagents for the assay of antibodies and antigens. (micrordt.com)
  • This type of EIA can be used only for assay of haptens such as drugs and not for microbial antigens and antibodies. (micrordt.com)
  • Research in the DDU resulted in the development of SAS, which is based on multiplying a visual signal via an increase in Format the valency and size of the coloured immune complex in the assay, by chemically coupling multiple copies of a hapten moiety to the primary detection antibodies. (ref.ac.uk)
  • Antibodies were prepared using different strategies of hapten conjugation, and the best hapten/antibody pair was selected. (fq.edu.uy)
  • Immunoassays for haptens depend on competitive hapten-anti-hapten reactions, and consequently their sensitivities are significantly influenced by the affinities of anti-hapten antibodies. (lu.se)
  • Thus, genetically engineered antibodies, which have much higher affinities than native antibodies, should increase assay sensitivities. (lu.se)
ELISA14
  • Enzyme-linked immunosorbent assays (ELISA) have been around as one of the primary methods of analyte detection for more than four decades. (ddw-online.com)
  • Over the years many changes to the basic format have resulted in assay improvements, but some of the most recent look set to take the traditional ELISA to new levels. (ddw-online.com)
  • These include many vendors now addressing the automation of ELISA assays. (ddw-online.com)
  • Other automated instrumentation is designed to support the creation/coating of ELISA assay plates or contains cartridge-based delivery of assay reagents to reduce set-up time. (ddw-online.com)
  • These are supported by a wide range of ELISA reagents, assay and kits providing relative profiling or absolute quantitation of proteins across a wide range of study areas. (ddw-online.com)
  • E nzyme-linked immunosorbent assays (ELISA) have been one of the primary methods of detecting the presence of a substance, usually an antigen, in an aqueous sample for more than 40 years. (ddw-online.com)
  • The immunoassay landscape has, however, dramatically changed in recent years with the development of reduced assay steps protocols, no wash ELISAs and new automated systems designed to run ELISA. (ddw-online.com)
  • This review article uses as its starting point some of the findings of HTStec's ELISA Assay Trends 2012 survey and report, published in June 2012 (1). (ddw-online.com)
  • This report discusses end-users' current practices and preferences in ELISA assays, and seeks to understand future user requirements. (ddw-online.com)
  • The main (primary) application area for survey respondents' ELISA assays was immunology (17% selecting). (ddw-online.com)
  • The Avidin-Biotin system has been utilized extensively for enzyme-linked immuno solid-phase assay (ELISA), in which an enzyme-Avidin conjugate (useful for detection by reaction with the enzyme's substrate to afford a colored or chemiluminescent product) is employed to detect the presence of a biotinylated antibody, after first binding the antibody to an immobilized antigen or hapten. (justia.com)
  • The major type of heterogeneous EIA is enzyme linked immunosorbent assay (ELISA). (micrordt.com)
  • Implementation of the immunochemical tools generated in several analytical platforms, including ELISA plate assays, immunoaffinity columns, lateral flow immunoassays (immunochromatographic strips) and biosensors. (haptens-antibodies.com)
  • The hapten hapten-carrier conjugates BSA-Carprofen had been validated with our anti-Hapten antibody Anti-Carprofen mouse monoclonal antibody via competitive ELISA test. (genemedi.net)
Antigen4
  • In addition, the antigen presentation should mimic the same orientation as the assay. (prosci-services.com)
  • The A1 antibody binds to the human CD39 cell surface antigen and has been shown to block MHC independent target cell recognition by hapten-specific CTL. (biolegend.com)
  • The term enzyme immunoassay (EIA) includes all assays based on the measurement of enzyme labelled antigen, hapten or antibody. (micrordt.com)
  • Kittelberger R., Diack D.S., Vizcaíno N., Zigmunt M.S., Cloeckaert A., Characterization of an immuno-dominant antigen in Brucella ovis and evaluation of its use in an Enzyme-Linked Immunosorbent Assay, Vet. (vetres.org)
Anti-hapten antibody2
  • These coated plates are an especially useful tool for assessing specific anti-hapten antibody titers during antibody production. (fishersci.com)
  • Competitive immunoassay-validated hapten-carrier conjugates BSA-Carprofen with anti-Hapten antibody. (genemedi.net)
Reagents2
  • Binding Assay Laboratories manufactures the radiolabeled 17β-estradiol binding assay reagents distributed by Genprice. (bioinfolab.org)
  • Pick up reagents and pre-defined assays for COVID-19, other viruses, and microbes. (biosearchtech.com)
Solid-phase assay2
  • detection of gene probes, enzyme-linked immuno solid-phase assay, monoclonal antibody drug targeting and medical imaging. (justia.com)
  • In other words, the number of different analytes that may be assayed in a single solid phase assay is limited by the solid phase. (justia.com)
Sensitivity7
  • A serological assay for HDI sensitivity was performed. (cdc.gov)
  • P erhaps most significantly are the big increases in assay sensitivity and the widening of the dynamic range accomplished by using alternatives to the traditional colorimetric detection, or by the use of new platform technologies. (ddw-online.com)
  • However, the ease-of-use and short assay time are achieved at the expense of sensitivity. (ref.ac.uk)
  • Two-step in vitro antibody affinity maturation enables estradiol-17β assays with more than 10-fold higher sensitivity. (lu.se)
  • ScFv#m2-c4 had more than 10-fold higher sensitivity (the midpoint of its dose-response curve was 0.56 ng) than Fab#E4-4 (midpoint 9.0 ng/assay) in a competitive E(2) radioimmunoassay, and even higher sensitivity [midpoint 21 pg/assay, and a limit of detection of 0.47 pg (1.7 fmol)/assay] in a competitive enzyme-linked immunosorbent assay. (lu.se)
  • By utilizing MST and BLI technologies, Creative Proteomics ensures the specificity and sensitivity of the aptamer-based sandwich assay, enabling accurate detection and quantification of target molecules. (iaanalysis.com)
  • 2019). These practical assays utilize the CLV molecule to judge whether the medication induces mobile activation, however they display suboptimal level of sensitivity (Ariza et al. (healthanddietblog.info)
Binding Assay2
  • The Radiolabeled 17Β-Estradiol Binding Assay reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (bioinfolab.org)
  • Description: The PCSK9[Biotinylated]-LDLR Binding Assay Kit is designed for screening and profiling purposes. (bioinfolab.org)
Immunosorbent assay5
  • Alonso-Urmeneta B., Marín C., Aragón V., Blasco J.M., Díaz R., Moriyón I., Evaluation of Lipopolysaccharides and Polysaccharides of different Epitope Structures in the Indirect Enzyme-Linked Immunosorbent Assay for Diagnosis of Brucellosis in Small Ruminants and Cattle, Clin. (vetres.org)
  • studied by Enzyme-Linked Immunosorbent Assay and Flow Cytometry, Clin. (vetres.org)
  • Marin C.M., Alonso-Urmeneta B., Moriyón I., Pérez-Gómez S., Blasco J.M., Comparison of polyclonal, monoclonal and Protein G peroxidase conjugates in an enzyme-linked immunosorbent assay for the diagnosis of Brucella ovis in sheep, Vet. (vetres.org)
  • Here, we report the development of a clomazone enzymelinked immunosorbent assay as a fast and cost-effective tool to monitor the dissipation of this herbicide along the harvest. (fq.edu.uy)
  • Fluorophore-linked immunosorbent assay: FITC Antibody [NB100-64617] - FLISA plate was coated with Goat Anti-Human IgG-FITC and Goat Anti-Human IgG-BIOT. (novusbio.com)
Detection1
  • One problem with conventional multiplex assays is that they typically cannot detect more than about five analytes simultaneously, because of difficulties with simultaneous detection and differentiation of more than about five analytes. (justia.com)
Monoclonal antibody1
  • The Mouse IgM G155-228 is a monoclonal antibody specific for trinitrophenol (TNP), a hapten not expressed on mammalian cells. (bdbiosciences.com)
Protein9
  • Thus, the principle of protein or peptide haptenation could be used in in vitro assays to predict the sensitization potential of a new chemical entity. (cdc.gov)
  • The basis of hapten- molecular mechanisms of the sensitization pro- protein binding work is the hypothesis that upon cess will result in novel opportunities for the skin absorption, only protein-reactive chemicals development of alternative methods for assessing (or those that can be metabolically or chemically skin sensitization hazard and relative potency of converted to protein-reactive species) are able to chemicals. (cdc.gov)
  • Screen Quest™ assay kits, a set of HTS-ready tools for high throughput screening of biochemical and cellular targets such as protein kinases, proteases, HDAC, cell apoptosis and cytoxicity, GPCR, ion channels, ADME/metabolism and transporters. (stratech.co.uk)
  • assay, FIA assay, protein /hapten binding etc. (custom-monoclonalantibody.com)
  • These immunogenic variables include the chemical structure, conjugation method, cross-linking between the small molecule and protein carrier, the orientation of the hapten on the carrier protein, and linkers used. (prosci-services.com)
  • Multiplex assays are performed to determine simultaneously the presence or concentration of more than one molecule in the sample being analyzed, or alternatively, several characteristics of a single molecule, such as, the presence of several epitopes on a single protein molecule. (justia.com)
  • BSA, ovalbumin (OVA), and horseradish peroxidase (HRP) had been utilized as carrier protein to get ready bioconjugates using the matching energetic esters of haptens SPand SPconjugates, and 17.9, 10.5, and 1.2 for BSACSPconjugates, respectively, had been obtained, which are equal to those of the published SPconjugates19 previously. (mycareerpeer.com)
  • The miRIDIAN microRNA Mimic Endogenous Positive Control helps you monitor specific mimic effects on target protein levels in a validated endogenous assay, which is based upon the targeted activity of miR-122 on Aldolase A mRNA levels in cell lines. (horizondiscovery.com)
  • Certain protein allergens and chemicals capable of acting as haptens occur at high concentrations in workplace environments. (practo.com)
Molecules4
  • In conventional solid-phase assays, the solid phase mainly aids in separating molecules that bind to the solid phase from molecules that do not bind to the solid phase. (justia.com)
  • Synthesis of functionalized derivatives (haptens) of organic molecules of interest in food quality and safety. (haptens-antibodies.com)
  • However, most drugs act as haptens, binding covalently to serum or cell-bound proteins, including peptides embedded in major histocompatibility complex (MHC) molecules. (msdmanuals.com)
  • Haptens may also bind directly to class II MHC molecules, directly activating T cells. (msdmanuals.com)
Proteins5
  • Thus, HERA is a novel cellular assay that can be used to predict how FcRn-binding proteins are rescued from intracellular degradation. (nature.com)
  • Haptens are chemicals that, alone, are too small to be immunogenic, but when bound to proteins, can elicit an immune response. (molecule-world.com)
  • Hapten activation and bioconjugate planning (S)-(-)-Bay-K-8644 to conjugation towards the carrier proteins Previously, haptens SPand SPwere turned on through the forming of the matching active (S)-(-)-Bay-K-8644 esters. (mycareerpeer.com)
  • 2017). In the framework of IgE-mediated reactions to -lactams, medicines work as haptens because they are assumed to covalently bind to carrier proteins to induce an immunological response (Ariza et al. (healthanddietblog.info)
  • FITC is widely used as a hapten to label different proteins. (novusbio.com)
Vitro3
  • For the end-point of skin sensitization, the ultimate goal, i.e. the development and validation of alternative in vitro/in silico assays by 2013, may be achieved through a better understanding of the skin sensitization process on the cellular and molecular levels. (cdc.gov)
  • Once sensitized local lymph node assay (LLNA) (6), as no vali- to that chemical, the person is susceptible to dated in vitro alternatives exist to date (7). (cdc.gov)
  • It is likely that should lead to useful insights for the development improving our understanding of the cellular and of in vitro assays (17, 18). (cdc.gov)
Colorimetric1
  • Maleimide Activated Plates are available in clear for colorimetric assays, white for chemiluminescent assays and black for fluorescent assays. (fishersci.com)
Conjugates1
  • These encompass various forms, such as Carrier-coupled antigens, immunogens, hapten-carrier conjugates, BSA-conjugated, and OVA-conjugated variants, designed to cater to diverse scientific needs. (genemedi.net)
Cellular1
  • Cell Meter™ cellular functional assay kits, a complete set of tools for functional analysis of cellular events. (stratech.co.uk)
Molecular1
  • A variety of molecular biology assays and purification methods employ biotin. (idtdna.com)
Probes3
  • This assay is used for methylation probes on the Illumina Infinium HumanMethylation27 BeadChip (henceforth, 27k [methylation] array). (wikipedia.org)
  • Disclosed are materials and methods for performing multiplex assays for nucleic acids, in which a transponder is associated with the bead(s) forming the solid phase used in the assay, nucleic acid probes are bound to the surface of the particles, and data concerning the assay is encoded on the transponder. (justia.com)
  • This invention relates to materials and methods for detecting nucleic acids in samples and, more particularly, to solid phase assays wherein transponders are associated with the beads constituting the solid phase, nucleic acid probes are bound to the surface of the particles and data concerning the assay is encoded on the transponders. (justia.com)
Peptide1
Analyte2
  • The resulting lattice formed between the analyte, multiple hapten- labelled antibody and the anti-hapten colour conjugate yields a strong visual signal. (ref.ac.uk)
  • In solid phase assays, small beads, or microparticles, are used as the solid phase to capture the analyte. (justia.com)
Antigens2
Clinical1
  • Here, we report on a novel human endothelial cell-based recycling assay (HERA) that can be used for such pre-clinical screening. (nature.com)
Skip1
Bead2
  • Anti-PEG IgE was measured using a dual cytometric bead assay (DCBA). (cdc.gov)
  • Anti-PEG IgG and IgM were measured using two different assays: DCBA and a PEGylated-polystyrene bead assay. (cdc.gov)
Molecule1
  • Sandwich assays involve the selection of two inducers that bind to different epitopes on the same target molecule. (iaanalysis.com)
Buffer1
  • When 100 micrograms HDI-HSA was incorporated into the assay buffer, binding was reduced to 1.4 percent. (cdc.gov)
Bind1
  • In an electronically-indexed multiplex assay of this invention, two or more classes of transponders, each encoded with a different index number and constructed to bind a different nucleic acid sequence, are incubated with the sample in a single vessel. (justia.com)
Serum2
  • The patient's serum was also positive in the RAST assay. (cdc.gov)
  • Using an assay that, uniquely, had been previously assessed in patients with anaphylaxis to PEG, our objective was to compare anti-PEG IgE in serum from mRNA COVID-19 vaccine anaphylaxis case-patients and persons vaccinated without allergic reactions. (cdc.gov)
Sandwich2
  • This Roche assay uses a sandwich principle. (cdc.gov)
  • We have specifically developed an aptamer-based sandwich assay using MST and BLI techniques. (iaanalysis.com)
Robust1
  • The Diagnostics Development Unit (DDU) was established in 1996 by Dr Helen Lee, Reader in Medical Biotechnology, Dept of Haematology (since 1991), who left the diagnostic industry with the goal of developing simple, high-performance, robust yet affordable assays for resource-limited settings. (ref.ac.uk)
Development1
Simplicity1
  • Because of its robustness and simplicity, the assay may be useful to delineate and monitor management practices that can contribute to minimizing the release of the herbicide in the environment. (fq.edu.uy)
Test2
  • Hemagglutination assay and hapten-sugar inhibition test were carried out to ascertain the blood group and sugar specificities of the lectin respectively. (edu.ng)
  • This reagent has been pre-diluted for use at the recommended Volume per Test when following the Recommended Assay Procedure. (bdbiosciences.com)
Target2
  • Aptamer characterization involves a set of techniques and assays aimed at elucidating the binding properties, kinetics, thermodynamics, and functional aspects of aptamer-target interactions. (iaanalysis.com)
  • These assays assess the ability of an inducer to disrupt a target-ligand complex or affect target-ligand interactions. (iaanalysis.com)
Interest2
  • Of significance, the resource also holds a wealth of assay data, so that a researcher can further evaluate the quality of an epitope of interest. (molecule-world.com)
  • Successful microRNA functional studies begin with optimization of the assay in each cell line and/or type of interest. (horizondiscovery.com)
Human1
  • The Illumina Methylation Assay using the Infinium I platform uses 'BeadChip' technology[clarification needed] to generate a comprehensive genome-wide profiling of human DNA methylation. (wikipedia.org)
Technology2
  • For the Infinium I assay chemistry technology, the process is outlined in Figure 1. (wikipedia.org)
  • AAT strive to innovate and push the boundaries of assay technology. (stratech.co.uk)