To evaluate for serum cryoglobulins, the blood specimen must be collected in warm tubes (37°C) in the absence of anticoagulants. (medscape.com)
Immunoglobulins1
Specific immunologic assays may be used to identify cryoglobulin components (immunoglobulins, light chains, clonality). (medscape.com)
Immunologic1
OBJECTIVE: To determine, using a serotyping assay, whether the occurrence of extrahepatic immunologic disorders in patients with chronic hepatitis C is dependent on hepatitis C virus serotype. (ox.ac.uk)
Precipitate1
MC is a both autoimmune and B-cell lymphoproliferative disorder characterized by immune complexes that reversibly precipitate at low temperature (cryoglobulins). (biomedcentral.com)
Quantification1
The limit of detection may be lower than the limit of quantification this assay is non linear at lower limits and will be reported as HCV RNA detected but below the limits of quantification. (activeboard.com)
Negative1
Antinuclear antibody and antineutrophil cytoplasmic antibody assays were negative. (who.int)
Deposits2
Renal biopsy revealed membranoproliferative glomerulonephritis, with lobulated glomerular tufts, endocapillary proliferation, thickened basement membrane, and glomerular hyaline thrombi suggestive of cryoglobulin deposits. (who.int)
Although biopsy samples generally exhibit inflammatory vascular changes (eg, leukocytoclastic vasculitis in patients with vasculitic purpura), intraluminal cryoglobulin deposits may be observed, especially in renal glomeruli. (medscape.com)
Concentration1
Cryoglobulin concentration may be determined via spectrophotometric analysis. (medscape.com)
Monoclonal1
The cryoglobulins were tested by immunofixation, which detected monoclonal IgM and polyclonal IgG with only traces of IgA (i.e. type II mixed cryoglobulinaemia) ( Figure 1 ). (who.int)
Antibodies2
PATIENTS: 59 consecutive patients with chronic hepatitis C. MEASUREMENTS: Hepatitis C virus serotype was determined using a recently developed immunoenzymatic assay that detects antibodies directed to serotype-specific immunodominant epitopes. (ox.ac.uk)
We then analysed the IgG component of the cryoglobulins and found it to be almost entirely composed of anti-adult worm antigen (AWA) Schistosoma spp. (who.int)
Testing his serum for hepatitis C viral mRNA by PCR would be unrevealing because any hepatitis C virus that was consumed within the cryoglobulin would be contained within the clot and not within the serum. (medscape.com)
Serum cryoglobulin and complement assays may be useful. (medscape.com)
1. Assay, isolation and characterization of circulating immune complexes from serum of gastrointestinal cancer, stage III and IV melanoma and chronic inflammatory bowel disease patients. (nih.gov)
Complement1
Complement assay -- complement levels will be low. (medlineplus.gov)
Antibody2
In this procedure, blood plasma is taken out of blood circulation and abnormal cryoglobulin antibody proteins are removed. (medlineplus.gov)
Antinuclear antibody and antineutrophil cytoplasmic antibody assays were negative. (who.int)
Titer1
To evaluate the prevalence of circulating cryoglobulins and to assess the relationship with clinical and virological parameters, 162 HIV-positive subjects (84 anti-HCV(+)) were tested for cryoglobulins, C3, C4, RF, autoantibodies, HIV-viral titer, and CD4(+) count. (nih.gov)
Cryoglobulinemia1
Although hepatitis C virus (HCV) is a recognized cause of circulating cryoglobulins, the role of human immunodeficiency virus (HIV) in the pathogenesis of cryoglobulinemia has not been investigated extensively. (nih.gov)
Laboratory1
We had the team obtain cryoglobulin, which was then prepared, washed, and sent to another laboratory for PCR testing to determine whether the cryoglobulin itself contained hepatitis C virus. (medscape.com)
Hepatitis4
This decision was based on a 1992 New England Journal of Medicine [ 1 ] article by Vincent Agnello and colleagues, who showed that the hepatitis C virus is concentrated 1000-fold within the cryoglobulin compared with the plasma. (medscape.com)
Presumably, because hepatitis C virus is a polyclonal activator of B cells, this virus is driving the patient's cryoglobulin production and, hence, its clinical manifestations. (medscape.com)
We recommended empiric treatment for hepatitis C virus infection pending the results of this assay. (medscape.com)
All patients were examined for the presence of signs or symptoms of vasculitis and tested for cryoglobulins using a standard biochemical assay. (nih.gov)
C3 (associated with pulmonary involvement in a subset of patients with urticarial vasculitis), C4 (sometimes low in hereditary angioedema), and C1-esterase inhibitor (associated with hereditary angioedema) functional assays may be performed. (medscape.com)
Immune2
Using research assays FDA and manufacturers found elevated levels of factor XIa, an activated clotting factor in certain lots of immune globulin products produced by different manufacturers. (blogspot.com)
18. Status of circulating immune complexes, IL8 titers and cryoglobulins in patients with dengue infection. (nih.gov)
Cold1
Cold urticaria: Ice no recoil crosshair challenge assay for cold agglutinins and cryoglobulins 2. (steelwood.cz)
Units1
The department hosts one of two Supraregional Assay Service Protein Reference Units in England UK and serves an extensive client base nationally and internationally, receiving samples from over 100 referring hospitals. (swlpath.nhs.uk)
Found2
Cryoglobulins were found in 30 (18.5%) cases. (nih.gov)
We then analysed the IgG component of the cryoglobulins and found it to be almost entirely composed of anti-adult worm antigen (AWA) Schistosoma spp. (who.int)
Protein1
The Protein Reference Unit maintains its specialist interest and skills in protein analysis, particularly relating to immunoglobulins, including cryoglobulins and heavy chain disease investigations. (swlpath.nhs.uk)
Plasma1
Plasma - Lithium Heparin sample is required for this assay. (southtees.nhs.uk)
Cancer1
Treatment is directed against the abnormal cancer cells that produce the cryoglobulin. (medlineplus.gov)
Analysis1
A novel quantitative hemolytic assay coupled with restriction fragment length polymorphisms analysis enabled early diagnosis of atypical hemolytic uremic syndrome and identified unique predisposing mutations in Japan. (cdc.gov)