• The number of live RGCs was then counted using a cell viability assay. (molvis.org)
  • Cell growth was measured using the CellTiter - Glo ® Luminescent Cell Viability Assay. (ashpublications.org)
  • Drugs were added as 10 stocks in press, and cell viability was measured in 72?h with CellTiter Blue Viability Assay (Promega, Madison, WI, USA). (synanet2020.com)
  • Level of sensitivity to medicines and effects of DUSP6 knockdown on DNA damage were assessed from the CellTiter Blue Viability Assay, immunofluorescence, and western blot. (synanet2020.com)
  • Movement Effects: According to cell viability assay, exosomes improve cell proliferation just after 24 h of treatment method for the two cell lines: MCF 10 A and A549 for all concentrations of exosomes (0.125, 0.25, 05, one mg/mL). (flapinhibitor.com)
  • The assay combines annexin V staining of PS and PE membrane events with the staining of DNA in the cell nucleus with propidium iodide (PI) or 7-Aminoactinomycin D (AAD-7), distinguishing viable cells from apoptotic cells and necrotic cells. (wikipedia.org)
  • Therefore, staining with FITC Annexin V is typically used in conjunction with a vital dye such as propidium iodide (PI) or 7-Amino-Actinomycin (7-AAD) to allow the investigator to identify early apoptotic cells (PI negative, FITC Annexin V positive). (bdbiosciences.com)
  • The rate of cell apoptosis was determined by annexin V-fluorescein isothiocyanate/propidium iodide double staining using fluorescence-activated cell sorting (FACS) analysis. (biomedcentral.com)
  • After 24 hr, the medium was replaced with fresh medium comprising DOX (0.9 binding buffer (0.1 M Hepes/NaOH (pH 7.4), 1.4 M NaCl, 25 mM CaCl2), additive supplemented with 5 of AV-412 FITC-Annexin V and 5 of propidium iodide (PI). (cgp60474.com)
  • Since intracellular annexin V is also exposed if the plasma membrane is compromised, a membrane-impermeant dye such as 7-AAD is commonly used to distinguish between apoptotic and dead cells to exclude the dead cells. (bdbiosciences.com)
  • Putative CSCs are isolated using methods based on several in vitro/in vivo assays, such as expression of distinct surface markers or intracellular enzyme activities, "sphere-forming" ability in non-adherent culture and/or initiation of new tumor growth when xenotransplanted into immunodeficient mice [ 8 ]. (oncotarget.com)
  • Annexin A7 translocates to membranes in a Ca 2+ -dependent fashion and, when intracellular Ca 2+ levels rise, sequentially redistributes to the plasma and the nuclear membrane as well as to intracellular vesicles. (biomedcentral.com)
  • The following methods were applied: at first proliferation assays with photometric analysis were conducted, then an annexin-V-detection was applied for differentiation of the treated cells in vital, necrotic and apoptotic groups. (uni-marburg.de)
  • Cell proliferationand cell apoptosis were detected by MTT assay and Annexin V-FITC/PI double staining, respectively. (moleculardepot.com)
  • BD Biosciences offers annexin V in several common formats such as FITC, PE, BD Horizon™ BV421 for the violet laser, and BD Horizon™ BUV395 for the ultraviolet laser. (bdbiosciences.com)
  • Flow Cytometric Analysis of FITC Annexin V staining. (bdbiosciences.com)
  • Untreated cells were primarily FITC Annexin V and PI negative, indicating that they were viable and not undergoing apoptosis. (bdbiosciences.com)
  • After a 4 hour treatment (bottom panels), there were primarily two populations of cells: Cells that were viable and not undergoing apoptosis (FITC Annexin V and PI negative) and cells undergoing apoptosis (FITC Annexin V positive and PI negative). (bdbiosciences.com)
  • A minor population of cells were observed to be FITC Annexin V and PI positive, indicating that they were in end stage apoptosis or already dead. (bdbiosciences.com)
  • Annexin V may be conjugated to fluorochromes including FITC. (bdbiosciences.com)
  • Since externalization of PS occurs in the earlier stages of apoptosis, FITC Annexin V staining can identify apoptosis at an earlier stage than assays based on nuclear changes such as DNA fragmentation. (bdbiosciences.com)
  • FITC Annexin V staining precedes the loss of membrane integrity which accompanies the latest stages of cell death resulting from either apoptotic or necrotic processes. (bdbiosciences.com)
  • buy 42971-09-5 Cells had been after that discolored with Annexin V-FITC and PI and examined for apoptosis relating to the manufacturer's guidelines. (informationalwebs.com)
  • After that, 100 d of this cell suspension system was incubated with 10 d of PI (50 g/ml) and 5 d of Annexin V-FITC for 15 minutes at space heat in the dark. (informationalwebs.com)
  • Cells that discolored positive for just Annexin V-FITC had been in the early stage of apoptosis, whereas cells that discolored positive for both Annexin V-FITC and PI had been in the stage of past due apoptosis or main necrosis. (informationalwebs.com)
  • Cells were washed in PBS and incubated for 15?moments at room temp in HEPES buffer remedy (10?mmol/L HEPES, pH 7.4, 140?mmol/L NaCl, 2.5?mmol/L CaCl2) with 2.5?L Annexin V Fitc/PI (BD Biosciences). (ourownfuture.org)
  • Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V". Journal of Immunological Methods. (wikipedia.org)
  • The populations of cells that are stained with annexin V only represent the apoptotic cell populations. (bdbiosciences.com)
  • The viability of cells treated with Cytarabine (AraC) alone or in combination with S63845 (a specific inhibitor of Mcl-1 anti-apoptotic protein) was assessed by PrestoBlue and Annexin V/PI assays. (unito.it)
  • Annexin V/ PI assay was employed to define the percentage of necrotic and apoptotic cell death soon after treating cancerous and non-tumorigenic cell lines. (flapinhibitor.com)
  • BD Biosciences also offers a range of tools for caspase activity assays from individual fluorogenic peptide substrates and inhibitors, to kits, to ready-to-use assay plates. (bdbiosciences.com)
  • The individual synthetic tetrapeptide substrates, together with the caspase inhibitors and active caspase enzymes, offer flexibility in the experimental design of a caspase activity assay. (bdbiosciences.com)
  • Several fluorogenic assays for caspase activity are available, including the PhiPhiLux system ,and the CellEvent™ Caspase 3/7 Green and NucView 488 Caspase substrates. (bitesizebio.com)
  • Impact of Trx inhibition on apoptosis was investigated by proteome profiling of a subset of proteins and annexin V apoptosis assays. (uni-koeln.de)
  • Active caspases can then cleave many proteins, including poly-ADP ribose polymerase (PARP), other caspases and other protein substrates en masse. (bdbiosciences.com)
  • The comet assay, cell cycle distribution, Annexin-V staining, and expression of proteins involved in DNA damage/repair were utilized to understand the mechanism underlying the effect of the combination. (uaeu.ac.ae)
  • 6 In addition, independent annexin V/Hoechst assays of primary T-PLL cells on NK-Tert stromal support demonstrated modest single-agent activity of ibrutinib ( Figure 1D , Online Supplementary Figure S2A ). (haematologica.org)
  • Annexin A5 (or annexin V) is a cellular protein in the annexin group. (wikipedia.org)
  • Inhibition of protein kinase C by annexin V". Biochemistry. (wikipedia.org)
  • Adenosine triphosphate, heat shock protein (HSP) 70, HSP90 and high mobility group box 1 (HMGB1) release were identified using bioluminescence, western blot and ELISA assays, respectively. (uanl.mx)
  • Annexin V is a 35-36 kDa Ca2+ dependent phospholipid-binding protein that has a high affinity for PS, and binds to cells with exposed PS. (bdbiosciences.com)
  • Collected data combined into a protein library for early diagnosis will lead to the improvement of cancer immunotherapy and new therapeutic targets. (flapinhibitor.com)
  • Annexin A7 is a Ca 2+ - and phospholipid-binding protein expressed as a 47 and 51 kDa isoform, which is thought to be involved in membrane fusion processes. (biomedcentral.com)
  • Annexin A7 is a Ca 2+ - and phospholipid-binding protein, which was isolated as the agent that mediated aggregation of chromaffin granules and fusion of phospholipid membranes in the presence of Ca 2+ . (biomedcentral.com)
  • Alpha v beta 5 integrin-dependent programmed cell death triggered by a peptide mimic of annexin V". Molecular Cell. (wikipedia.org)
  • Analysing the results of the proliferation assays synergistic effects when combining Flavorpiridol and Lonafarnib could not be shown in SKOV3-cells but in BG1-cells. (uni-marburg.de)
  • Wu M, Sun Y, Xu F, Liang Y, Liu H, Yi Y. Annexin A2 Silencing Inhibits Proliferation and Epithelial-to-mesenchymal Transition through p53-Dependent Pathway in NSCLCs. (jcancer.org)
  • Moreover, we found that knockdown of Annexin A2 significantly suppressed cell proliferation and invasion of NSCLCs cells. (jcancer.org)
  • Extensive researches demonstrate that Annexin A2 plays important roles in tumor proliferation, invasion and metastasis [ 12 - 14 ]. (jcancer.org)
  • Knockdown of Annexin A2 significantly suppressed cell proliferation and invasion of NSCLCs cells, and the biological effect of Annexin A2 on proliferation and invasion was mediated by the inhibition of p53, which in turn, promoted cell cycle progression and EMT. (jcancer.org)
  • Our results suggest that the combination of chidamide and bortezomib can significantly reduce the proliferation, invasion, and migration of MGC-803 and BGC-823 cells, providing a framework for the clinical evaluation of combined therapies for gastric cancers. (dovepress.com)
  • Furthermore, the IncuCyte Annexin V Reagents can be combined with the IncuCyte ZOOM confluence metric, IncuCyte Caspase 3/7 Reagent, IncuCyte NucLight nuclear labeling reagents or IncuCyte Cytotox Reagents for multiplexed measurements of apoptosis, cell proliferation or cytotoxicity in every assay well. (accela.eu)
  • To further explore the effects of BCL11A siRNA on the enhanced cytotoxicity of a chemotherapeutic drug, we investigated the effects of BCL11A siRNA combined with vincristine (VCR) on SUDHL6 cell proliferation and apoptosis. (biomedcentral.com)
  • Cell proliferation was measured by the CCK8 assay. (biomedcentral.com)
  • Cell viability, based on mitochondrial dehydrogenase activity, was determined by colorimetric assay using a MTT Cell Proliferation Kit (Roche Applied Technology, Mannheim, Germany) in accordance with the instruction manual. (cgp60474.com)
  • Cell proliferation assay, cell\cycle analysis and assessment of apoptosis Cells were plated in 96\well plates in the denseness of 1 1.5? (ourownfuture.org)
  • Apoptosis was measured by circulation cytometry after staining with Annexin V. Briefly, after 2?days with or without JQ1 (0.5?mol/L), venetoclax (0.5?mol/L) or a combination of these two medicines. (ourownfuture.org)
  • Data were analyzed with the CellQuest WinMDI software (BD Biosciences, San Jose, CA, U.S.A.). in 24-well smooth bottom microtiter plates and cultivated and treated as explained for an apoptosis assay. (cgp60474.com)
  • Annexin V and TUNEL assays Apoptosis was measured with the Annexin V surface labelling, DNA fragmentation was assessed from the TUNEL assay (both using assay packages from Guava Systems, Hayward, CA, USA). (synanet2020.com)
  • Distinct hallmarks of cancer were examined using in silico analysis, various in vitro cell culture assays, and ex vivo organotypic rat brain slice culture model. (hindawi.com)
  • We investigated the impact on tumor malignancy in F98 glioma cells using in silico analysis, in vitro cell culture assays, and ex vivo vascular organotypic glioma impact model (VOGIM) [ 35 ] by implanting F98 cells into rat brain slices. (hindawi.com)
  • The IncuCyte Annexin V Reagents are highly-selective phosphatidylserine (PS) cyanine fluorescent dyes. (accela.eu)
  • The results appear to rule out an important role of annexin A7 in membrane fusion processes occurring in red blood cells. (biomedcentral.com)
  • The defects observed are however not compatible with a crucial role for annexin A7 in membrane fusion processes in these cell types. (biomedcentral.com)
  • Finally, the inhibitory effect of the combined chidamide and bortezomib treatment on MGC-803 cells was verified in vivo through tumor formation experiments in nude mice. (dovepress.com)
  • PS exposure on the extracellular surface enables binding of the IncuCyte Annexin V Reagent resulting in a bright and photostable fluorescent signal. (accela.eu)
  • Cells were combined with HiPerFect transfection reagent (Qiagen Inc.) and siRNAs in 1% FBS-DMEM and after 24?h followed by treatment with medicines. (synanet2020.com)
  • however, annexin A5 has been proposed to play a role in the inhibition of blood coagulation by competing for phosphatidylserine binding sites with prothrombin and also to inhibit the activity of phospholipase A1. (wikipedia.org)
  • Combined inhibition of Trx and the phosphatidylinositide 3-kinase (PI3K) pathway resulted in massive cell death. (uni-koeln.de)
  • Annexin A5 forms a shield around negatively charged phospholipid molecules. (wikipedia.org)
  • The annexin A5 affinity assay typically uses a conjugate of annexin V and a fluorescent or enzymatic label, biotin or other tags, or a radioelement, in a suitable buffer (annexin V binding to aminophospholipids is Ca2+ dependent). (wikipedia.org)
  • Annexin-FP488 fluorescent staining protocol at Interchim Wen Y, Edelman JL, Kang T, Sachs G (May 1999). (wikipedia.org)
  • Addition of the IncuCyte Annexin V Reagents to normal healthy cells is non-perturbing to cell growth and morphology and yields little or no intrinsic fluorescent signal. (accela.eu)
  • Clinicopathologic analysis showed that Annexin A2 expression was significantly correlated with clinical stage, and lymph node metastasis. (jcancer.org)
  • Kaplan-Meier analysis revealed that patients with high Annexin A2 expression had poorer overall survival rates than those with low Annexin A2 expression. (jcancer.org)
  • Annexin V/PI staining and Western blot analysis were used to characterize cell death. (biomedcentral.com)
  • Essen BioScience & Sartorius is a team of engineers and biologists with deep expertise in cell-based assays and biomolecular interaction analysis. (accela.eu)
  • The influence of the sorcin/annexin A7 interaction on the ryanodine receptor is unknown. (biomedcentral.com)
  • Annexin A5 is used as a non-quantitative probe to detect cells that have expressed phosphatidylserine (PS) on the cell surface, an event found in apoptosis as well as other forms of cell death. (wikipedia.org)
  • Radio-frequency dose-dependent apoptosis, necrosis and cell death monitored by annexin V-BD Horizon™ V450. (bdbiosciences.com)
  • Radio-frequency dose-dependent apoptosis, necrosis and cell death monitored by annexin V-BD Horizon™ V450 in pancreatic carcinoma cell lines treated with a low dose of cetuximab targeted gold nanoparticles. (bdbiosciences.com)
  • The binding of annexin A7 and sorcin is Ca 2+ -dependent and occurs at micromolar Ca 2+ concentrations. (biomedcentral.com)
  • Therefore, a combined therapy that targets both the tumor cell and the CAFs might lead to novel therapies in cancer. (biomedcentral.com)
  • Hence, our study indicates that a combined exposure to Etoposide and Bevacizumab targets melanoma cells endowed with stem-like properties and might be considered a novel approach to treat cancer-initiating cells. (oncotarget.com)
  • Assuming for simplicity a single available and a single non available state, we MAPK assay estimated the lifetimes of the available and non available gating processes. (mdmsignaling.com)
  • Red blood cells may represent an appropriate model to study the role of annexin A7 in cellular processes. (biomedcentral.com)
  • in BG1-cells combining both drugs led to a rise of necrosis and a reduction of vital cells. (uni-marburg.de)
  • Etoposide does not influence phenotype of this sub-population of melanoma cells, while a combined treatment with Etoposide and Bevacizumab significantly abolished P1 sphere-forming ability, an effect associated with apoptosis of this subset of cells. (oncotarget.com)
  • IncuCyte Annexin V Reagents enable real-time evaluation of PS exposure in response to pharmacological agents and/or genetic and environmental factors. (accela.eu)
  • Mechanistically, our studies showed that knockdown of Annexin A2 increased the expression of p53, which in turn, induced cell cycle G2 arrest and inhibited epithelial-to-mesenchymal transition (EMT). (jcancer.org)
  • Putative CSCs were isolated from human melanoma cells of a different aggressiveness, Hs294T and A375 cell lines, grown under hypoxia using "sphere-forming assay", CD133 surface expression and migration ability. (oncotarget.com)
  • However, the exact function and mechanism of Annexin A2 in tumor progression of NSCLCs have not been elucidated. (jcancer.org)
  • However, the exact function and mechanism of Annexin A2 in tumor progression of NSCLCs need to be further determined. (jcancer.org)
  • Annexin A2 has been involved in cancer cell adhesion, invasion and metastasis. (jcancer.org)
  • This activity led to the proposal of annexin A7's involvement in the exocytotic secretion of catecholamines [ 1 ]. (biomedcentral.com)
  • Antibodies directed against annexin A5 are found in patients with a disease called the antiphospholipid syndrome (APS), a thrombophilic disease associated with autoantibodies against phospholipid compounds. (wikipedia.org)
  • The combined treatment of Flavopiriol and Lonafarnib in BG1-cells showed a slightly increased cell cycle arrest in the subG1-fraction in selected concentrations. (uni-marburg.de)
  • These data suggest that the combined CDK7 inhibitor THZ1 and antiPD-1 therapy can be an effective treatment in NSCLC. (biomedcentral.com)
  • The result of treatment with a combined mix of metformin and TRAIL was looked into in a number of CRC cell lines aswell as FHC cells. (healthweeks.com)
  • Combined treatment with S63845 and AraC restored the sensitivity of CD157-positive cells. (unito.it)
  • It could thus be of interest to further combine this treatment with immunotherapy. (breastcancertalk.net)
  • After the 72 hr treatment, the floating and adherent cells were combined for the analyses. (cgp60474.com)
  • CD8 depletion antibodies were used to assess the role of CD8 + T cells in combined CDK7 and PD-1 blockade. (biomedcentral.com)
  • The role of annexin A7 in red blood cells was addressed in erythrocytes from anxA7 -/- mice. (biomedcentral.com)
  • With the addition of these new formats, more complex assays can be developed to look at apoptosis within heterogeneous cell subsets. (bdbiosciences.com)
  • The formation of an annexin A5 shield blocks the entry of phospholipids into coagulation (clotting) reactions. (wikipedia.org)
  • As a result, Annexin A2 normally contributes to clearance of fibrin formation on the blood vessel surface in the case of minor vascular injury [ 8 ]. (jcancer.org)
  • The growth inhibitory effect of the proposed combination given in different sequences was assessed using the colony formation assay. (uaeu.ac.ae)
  • Resistance of GBM cell lines and primary cells to drugs and signaling inhibitors was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays. (uni-koeln.de)
  • The combined results of three experiments carried out using triplicate wells in each experiment were analyzed. (ashpublications.org)
  • Crystal and molecular structure of human annexin V after refinement. (wikipedia.org)