ELISACGRPReceptorsCalcium and phosphateReceptorReagentSalmonProcalcitonin assaySecretionGeneHormoneAntibodiesVitroHormonesMalignancyImmunosorbentIntracellularAminoThyrocalcitoninThyroidGlucoseFerritinProteinAntibodyConcentrationPeptidesHyperthyroidismPaget'sPrecursorsSerum of healthyClinicalRenalFunctionalRegulationRatsUrineAnalysesSensitivityElecsysHighDiseasesAdequate
ELISA3
- End-point analyses, like ELISA, are less informative and rely upon labeling the reactants and adsorbing one of them, which can spoil their native activity, give high non-specific background signals, and limit the scope of an assay. (intechopen.com)
- Enzyme-linked immunosorbent assay (ELISA): Use the primary antibody for capture, and conjugate the secondary antibody with an enzyme or radioisotope for detection. (creative-proteomics.com)
- Calcitonin is assessed in clinical laboratories using noncompetitive immunoassays of different formats: enzyme-linked immunosorbent assay (ELISA), radioimmunoassay (RIA), and chemiluminescent immunoassays. (medscape.com)
CGRP6
- The aim of the present study was to examine the effect of the synthetic endocannabinoid (eCB) analogue, arachidonyl-2′-chloroethylamide (ACEA), on calcitonin gene-related peptide (CGRP) release in the dura and trigeminal ganglion (TG), as cannabinoids are known to activate G i/o -coupled cannabinoid receptors type 1 (CB1), resulting in neuronal inhibition. (lu.se)
- CGRP release was induced by either 60 mM K + (for depolarization-induced stimulation) or 100 nM capsaicin (for transient receptor potential vanilloid 1 (TRPV1) -induced stimulation) and measured using an enzyme-linked immunosorbent assay. (lu.se)
- In the present study, we investigated the effect of LPS on the releasing function of afferent terminals as measured by calcitonin gene-related peptide (CGRP) release in ex vivo perfused rat trachea, and examined the possible role of the cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) as intermediaries in this effect. (jneurosci.org)
- Aside from the generation of action potentials, the C-fiber terminal is a secretory system from which peptides such as calcitonin gene-related peptide (CGRP) and tachykinins are released ( Holzer, 1988 ). (jneurosci.org)
- Biochemical and functional evidence suggest that the calcitonin receptor-like receptor (CRLR) interacts with receptor activity-modifying protein-1 (RAMP1) to generate a calcitonin gene-related peptide (CGRP) receptor. (acs.org)
- In addition to shed new light on the CRLR−RAMP1 signaling complex, the BRET assays developed herein offer new biosensors for probing CGRP receptor activity. (acs.org)
Receptors3
- Osteoblasts do not have calcitonin receptors and are therefore not directly affected by calcitonin levels. (wikipedia.org)
- Ganglion-blocker pentamin (2.5 mg/100g body weight), blocker of M-cholino-receptors atropine (0.2 ml), α-adreno-blocker tropaphen (0.1 mg/100g), and β-adreno-blocker obzidan (0.1 mg/100g) evoked the inhibiting effect on calcitonin secretion in spite of simultaneously increasing of hypog-lycemia. (scirp.org)
- Furthermore, they have proved themselves in assays with other membrane receptors, such as receptor tyrosine kinases. (tansobio.com)
Calcium and phosphate3
- In many ways, calcitonin counteracts parathyroid hormone (PTH) and vitamin D. More specifically, calcitonin lowers blood Ca2+ levels in two ways: Major effect: Inhibits osteoclast activity in bones, which break down the bone Minor effect: Inhibits renal tubular cell reabsorption of Ca2+ and phosphate, allowing them to be excreted in the urine High concentrations of calcitonin may be able to increase urinary excretion of calcium and phosphate via the renal tubules. (wikipedia.org)
- The full spectrum of calcitonin regulation is not completely understood, but its secretion is primarily regulated by the ionized calcium concentration, with increases in ionized calcium leading to increases in calcitonin, while pharmacological doses of calcitonin reduce serum calcium and phosphate concentrations by inhibiting osteoclastic bone resorption and reducing renal tubular reabsorption. (medscape.com)
- Together with vitamin D and calcitonin, PTH brings about the mobilization of calcium and phosphate from the skeletal system and increases the uptake of calcium in the intestine and the excretion of phosphate via the kidneys. (cdc.gov)
Receptor5
- The development and optimisation of an HPLC-based in vitro serum stability assay for a calcitonin gene-related peptide receptor antagonist peptide. (iasp-pain.org)
- The calcitonin receptor is a G protein-coupled receptor localized to osteoclasts as well kidney and brain cells. (wikipedia.org)
- Physiological mechanisms of the stimulating effect of insulin hypoglycemia on calcitonin secretion were studied in double-side adrenalectomized and pancreatectomized rats and under the blocking synaptic transmission in sympathetic ganglions or via peripheral cholino- and adreno-receptor structures. (scirp.org)
- Language": "en", "Country": "XG", "Code": "Content" }, { "Name": "Intended Use", "Value": "VENTANA pan-TRK (EPR17341) Assay is intended for laboratory use in the qualitative immunohistochemical detection of the C-terminal region of the tropomyosin receptor kinase (TRK) proteins A, B and C by light microscopy in sections of formalin-fixed, paraffin-embedded tissue stained on a BenchMark IHC/ISH instrument. (roche.com)
- The all-around Gα protein cocktail complements our proprietary assay cells and enables functional assays for any GPCR under a single protocol, regardless of the Gα protein the target receptor is coupled with. (tansobio.com)
Reagent5
- The assay is designed to provide full automation, which means no reagent preparation or hands-on testing is required. (genomeweb.com)
- Theoretically, this issue is only applicable to sandwich immunometric assays without a wash step between reagent additions. (lww.com)
- But, in all sandwich assays, the signal begins to plateau with high concentrations of analyte due to limiting amounts of reagent antibodies and rare samples with extremely high concentrations of analyte can even lead to the hook effect in assays with a wash step. (lww.com)
- The Procalcitonin Calcitonin And reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (putyourcolours.pl)
- Language": "en", "Country": "XG", "Code": "Storage Conditions (Product)" }, { "Name": "Content", "Value": "VENTANA pan-TRK (EPR17341) Assay contains sufficient reagent for 50 tests. (roche.com)
Salmon5
- Salmon calcitonin is used for the treatment of: Postmenopausal osteoporosis Hypercalcaemia Bone metastases Paget's disease Phantom limb pain It has been investigated as a possible non-operative treatment for spinal stenosis. (wikipedia.org)
- The following information is from the UK Electronic Medicines Compendium Salmon calcitonin is rapidly absorbed and eliminated. (wikipedia.org)
- Concomitant use of calcitonin salmon and lithium may lead to a reduction in plasma lithium concentrations due to increased urinary clearance of lithium. (drugs.com)
- There is no evidence that the prophylactic use of calcitonin salmon is beneficial in asymptomatic patients. (drugs.com)
- The evidence of efficacy for calcitonin salmon injection is based on increases in total body calcium observed in clinical trials. (drugs.com)
Procalcitonin assay1
- The Roche procalcitonin assay assesses risk when somebody presents with sepsis-related symptoms. (genomeweb.com)
Secretion6
- Secretion of calcitonin is stimulated by: an increase in serum [Ca2+] gastrin and pentagastrin. (wikipedia.org)
- The increasing secretion of calcitonin and hypocalcemia under insulin hypoglycemia, induced with insulin injection (1 IU/100g), was established. (scirp.org)
- Insulin hypoglycemia didn't expose the increasing secretion of calcitonin in rats under adrenalectomy and pancreatectomy. (scirp.org)
- Corticosteroids and, obviously, glucagon and also the tone of autonomic nervous system via peripheral M-cholinoreactive and α- and β-adrenoreactive structures take part in the activation of calcitonin secretion under insulin hypoglycemia. (scirp.org)
- Moisa, S. and Nozdrachev, A. (2014) Calcitonin Secretion under Insulin Hypoglycemia. (scirp.org)
- Butakova (Moisa), S.S. (2005) Calcitonin Secretion under the Different State of Carbohydrate Metabolism in Ontogenesis in Rats. (scirp.org)
Gene9
- Here, we describe the optimisation and validation of a method for the evaluation of the stability of a lead calcitonin gene-related peptide antagonist peptide (P006) in blood serum. (iasp-pain.org)
- citation needed] Calcitonin is formed by the proteolytic cleavage of a larger prepropeptide, which is the product of the CALC1 gene (CALCA). (wikipedia.org)
- The CALC1 gene belongs to a superfamily of related protein hormone precursors including islet amyloid precursor protein, calcitonin gene-related peptide, and the precursor of adrenomedullin. (wikipedia.org)
- Calcitonin also has significantly impacted molecular biology, as the gene encoding calcitonin was the first gene discovered in mammalian cells to be alternatively spliced, now known to be a ubiquitous mechanism in eukaryotes. (wikipedia.org)
- 2003. In vitro and in vivo calcitonin I gene expression in parenchymal cells: a novel product of human adipose tissue. (aacc.org)
- Aims: To define the effect of the neuropeptides substance P calcitonin gene related peptide vasoactive intestinal polypeptide neuropeptide Y and secretoneurin on the PluriSln 1 proliferation of human retinal pigment epithelial (RPE) cells. (conferencedequebec.org)
- Nerve fibers were assayed structurally by protein gene product 9.5 (PGP9.5) nerve immunostaining. (cdc.gov)
- After treatment, rats were euthanized, and tail tissues were chemically fixed for histological analysis of nerves (PGP9.5, neuropeptide Y, calcitonin gene related protein) and mast cells (avidin, Alcian Blue/Safranin O).2 Single sections were doublestained for nerve fibers and mast cells to permit assessment of the degree of physical overlap. (cdc.gov)
- Our proprietary assay cells are equipped with an artificial gene circuit that amplifies GPCR-induced intracellular signals, resulting in highly sensitive and specific results. (tansobio.com)
Hormone4
- Calcitonin is a 32 amino acid peptide hormone secreted by parafollicular cells (also known as C cells) of the thyroid (or endostyle) in humans and other chordates in the ultimopharyngeal body. (wikipedia.org)
- However, some diagnostics firms are still betting on a more well-established approach to diagnosing sepsis - measuring patient levels of procalcitonin, a peptide precursor of the hormone calcitonin. (genomeweb.com)
- Butakova (Moisa), S.S. and Nozdrachev, A.D. (2010) Calcitonin-Glucoregulating Hormone. (scirp.org)
- Procalcitonin is a precursor of the hormone calcitonin, which is detectable in the serum of healthy, non-infected individuals when measured with commercial sensitive PCT assays (2). (aacc.org)
Antibodies2
- formerly, the antimicrosomal antibodies and antithyroglobulin), thyroglobulin, sedimentation rate, and calcitonin in an individual at high risk for medullary carcinoma of the thyroid. (medscape.com)
- We work to bring you antibodies and antigens of the highest quality for your IVD assay development needs. (biospacific.com)
Vitro1
- ProductData": { "ID": "RTD001230", "ProductType": "Others", "BrandName": "VENTANA ® pan-TRK (EPR17341) Assay", "ProductNameAddition": "", "RegulatoryDisclaimer1": "For in vitro diagnostic use. (roche.com)
Hormones7
- Moisa, S.S. and Nozdrachev, A.D. (2014) Calcitonin and Parathyrin Are Glucoregulating Hormones. (scirp.org)
- Other potent calcitonin secretagogues include the gastrointestinal peptide hormones, gastrin in particular. (medscape.com)
- Identification of a-amidated hormones from a biological source has been severely hindered by the lack of a specific assay for this distinctive class of biological hormones, indicating that numerous a-amidated hormones remain undiscovered. (usf.edu)
- Based on the selective in situ chemistry of PAM, a novel and specific assay was developed for the discovery of a-amidated hormones. (usf.edu)
- The medium was fractionated by chromatographic techniques and each specific fraction was then assayed by the newly developed platform technology for the presence of a-amidated hormones. (usf.edu)
- Based on this 1:1 molar ratio, several novel spectrophotometric, fluorescent, and chemi-luminescent enzyme linked assays for glyoxylate were developed, which when utilized on cell culture fractions proved positive for the identification of a-amidated hormones. (usf.edu)
- Moreover the assay was utilized to identify two known a-amidated hormones accumulated from cell culture, which were further verified by Mass Spectral analysis. (usf.edu)
Malignancy1
- The rise in geriatric population will further compound the growing demand for malignancy assays and the rapid market expansion. (aarkstore.com)
Immunosorbent1
- 25 ] In India, the laboratory methods commonly followed are immunometric and enzyme-linked immunosorbent assays where the prevalence of hook effect is more. (lww.com)
Intracellular2
- PAM was equally active when targeted to each intracellular location and assayed in homogenates. (biomedcentral.com)
- The assay allows both end-point and kinetic measurement of intracellular cAMP changes with a FLIPR or a fluorescence microplate reader. (eenzyme.com)
Amino1
- Precalcitonin (116 amino acids) is cleaved to procalcitonin, which is further cleaved to immature calcitonin (33 amino acids) and then to mature calcitonin, a monomer of a 3.5-kd peptide composed of 32 amino acids, which is the only biologically active form. (medscape.com)
Thyrocalcitonin2
- Historically calcitonin has also been called thyrocalcitonin. (wikipedia.org)
- Laljee, H.C.K., Smith, K.I. and Dorrington, K.J. (1967) The Assay of Human Thyrocalcitonin in Mice. (scirp.org)
Thyroid4
- It is also a short-lived effect because the kidneys become resistant to calcitonin, as demonstrated by the kidney's unaffected excretion of calcium in patients with thyroid tumors that secrete excessive calcitonin. (wikipedia.org)
- Calcitonin assay is used in identifying patients with nodular thyroid diseases. (wikipedia.org)
- Medullary thyroid cancer, typically produces an elevated serum calcitonin level. (wikipedia.org)
- Diagnosis and monitoring of medullary thyroid carcinoma (MTC) is the main application of the serum calcitonin assay. (medscape.com)
Glucose3
- Butakova (Moisa), S.S. and Nozdrachev, A.D. (2010) Effect of One-Time Injection of Calcitonin Preparations on Glucose and Calcium Level in Rats of Different Age Groups. (scirp.org)
- Moisa, S.S. (2013) Calcitonin Contra-Insulin Action on Glucose Metabolism. (scirp.org)
- Moisa, S.S. and Nozdrachev, A.D. (2013) One-Time Injection of Calcitonin Induces Glucose Intolerance in Children with the 1st Degree Obesity. (scirp.org)
Ferritin1
- 12 ] in the early 1970s with a two-site immunoradiometric assays (IRMA) for ferritin. (lww.com)
Protein6
- It belongs to the calcitonin-like protein family. (wikipedia.org)
- Figure 1 depicts the expected assay results for a 1:1 protein-protein interaction. (bmglabtech.com)
- VENTANA pan-TRK (EPR17341) Assay is produced as a Protein A purified recombinant rabbit monoclonal antibody. (roche.com)
- This cutting-edge technology enables functional assays of all GPCRs regardless of coupled G-protein subtypes, making it a powerful tool in drug discovery enterprises. (tansobio.com)
- Our core technology combines sensitive and versatile reporter cells, a comprehensive Gα protein cocktail, and a comprehensive human and mouse GPCR library to make possible functional assays of all GPCRs. (tansobio.com)
- The core technology for our cell-based GPCR functional assay consists of sensitive and versatile test cells, a Gα protein cocktail, and an exhaustive human GPCR library. (tansobio.com)
Antibody3
- Human IgG antibody Laboratories manufactures the procalcitonin calcitonin and reagents distributed by Genprice. (putyourcolours.pl)
- One 5 mL dispenser of VENTANA pan-TRK (EPR17341) Assay contains approximately 140 μg of a rabbit monoclonal antibody. (roche.com)
- Language": "en", "Country": "XG", "Code": "Intended Use" }, { "Name": "Background Information", "Value": "VENTANA pan-TRK (EPR17341) Assay is a rabbit monoclonal primary antibody directed against the C-terminal region of TRK A, B and C proteins, also known as pan-TRK. (roche.com)
Concentration2
- Selochnik, I.I., Briskin, A.I. and Antonova, E.E. (1978) Photoelectrocolorimetric Assay of Calcium Concentration in Serum by the Help of EDTA and Murexide. (scirp.org)
- Age, pregnancy, lactation, and ingestion of food have been reported to influence calcitonin concentration in healthy individuals, but specific reference intervals have not been established. (medscape.com)
Peptides1
- These results are puzzling, since peptides terminating with -Phe-Gly are far better substrates for PAM that peptides terminating with -Pro-Gly, using test tube assays and purified enzyme [ 1 ]. (biomedcentral.com)
Hyperthyroidism1
- Given the sensitive third-generation assays, in the absence of symptoms of hyperthyroidism or hypothyroidism, further testing is not required. (medscape.com)
Paget's1
- The goal of treating Paget's disease with antiresorptive agents such as the bisphosphonates and calcitonin is to normalize the serum alkaline phosphatase and urine N-telopeptide levels. (sagepub.com)
Precursors1
- Calcitonin is derived from larger precursors. (medscape.com)
Serum of healthy1
- Multiple forms of circulating calcitonin have been found in the serum of healthy and diseased individuals. (medscape.com)
Clinical2
- This report provides a granular strategic analysis of over 55 clinical chemistry assays. (marketresearch.com)
- Test volume and sales forecasts for over 55 clinical chemistry assays. (marketresearch.com)
Renal1
- However, calcitonin has a role in the elderly patient with renal insufficiency in whom relief of pain rather than remission is a reasonable outcome. (sagepub.com)
Functional4
- Radioligand binding and cAMP production assays also confirmed that the fusion proteins retained normal functional properties. (acs.org)
- Tanso Biosciences has developed a high-performance functional assay to overcome the barriers in GPCR drug discovery. (tansobio.com)
- Current binding assays suffer from false positives and false negatives, and functional assays have limitations, such as being time-consuming, expensive, or limited in their applicability. (tansobio.com)
- Tanso Biosciences has developed a high-performance functional assay to overcome these barriers and make high-hanging GPCRs accessible to the pharmaceutical industry. (tansobio.com)
Regulation1
- Also, calcitonin inhibits food intake in rats and monkeys, and may have CNS action involving the regulation of feeding and appetite. (wikipedia.org)
Rats3
- Butakova (Moisa), S.S. and Nozdrachev, A.D. (2010) Effect of Calcitonin on the Type of Alimentary Hyperglycemia in Rats of Different Age and Sex. (scirp.org)
- Butakova (Moisa), S.S. (2007) Serum Calcitonin Activity and Total Calcium Content under the Different State of Carbohydrate Metabolism in Ontogenesis in Rats. (scirp.org)
- Lineberry, M.P. and Waite, L.C. (1978) Calcitonin Responses in Intact and Adrenalectomized Rats. (scirp.org)
Urine1
- Spot urine assays for N-telopeptide levels have replaced 24-h urine collections for hydroxyproline as a measure of antiresorptive activity. (sagepub.com)
Analyses1
- Nevertheless, recent meta-analyses have questioned the value of PCT in some conditions (7), potentially due to lack of standardization and harmonization of the assays used between different studies. (aacc.org)
Sensitivity2
- The assays are heterogeneous, with different sensitivity and specificity, and often they recognize multiple forms of immunoreactive calcitonin. (medscape.com)
- Our assay has yielded consistent readouts with results from existing methods and at high sensitivity and S/B ratio. (tansobio.com)
Elecsys1
- Total duration of assay is 9 minutes on the Elecsys 1010. (cdc.gov)
High5
- Although high correlation was observed between most assays, highly variable bias remains among immunoassays. (aacc.org)
- High-dose effect is a common phenomenon documented in various immunological and serological assays, which results in false-negative results. (lww.com)
- More than 60 years ago, the phenomenon of high-dose hook effect had been documented in serological assays, which resulted in false-negative results. (lww.com)
- High-dose hook effect still remains a problem in many assay techniques like one-step immunometric assays, [ 2 3 4 5 6 7 8 9 ] immunoturbidimetric assays, [ 10 ] and immunonephelometric assays. (lww.com)
- Basal calcitonin levels are high in most patients with sporadic MTC but are normal in 30% of those with familial MTC (FMTC) or multiple endocrine neoplasia (MEN) type 2. (medscape.com)
Diseases1
- A wide range of oncology assays to help clinicians fight the most threatening diseases. (siemens-healthineers.com)
Adequate1
- The lack of adequate and reliable assays has been a barrier to GPCR drug discovery. (tansobio.com)