OocytesVitroMurineBlastocystsUterusVivoMaternalPregnancyEmbryonicMorulaEndometriumELISAInduceDevelopmentalSerumViabilityMethodsPost-fertilizationDevelopmentSignificantlyGenesSpeciesEarly1999ProliferativeSpermatozoaSingleMouseHumanMiceActivityMeioticMediumResultsMultipleSupportModelsFailureDetermineMajor
Oocytes5
- Effect of autophagy induction and cathepsin B inhibition on developmental competence of poor quality bovine oocytes. (researchmap.jp)
- Dynamic status of lysosomal cathepsin in bovine oocytes and preimplantation embryos. (researchmap.jp)
- Collected MII oocytes were vitrified and divided into four groups: untreated, 10 mM caffeine (CA), 10 μM MG132 (MG), and 10 mM caffeine +10 μM MG132 (CA+MG). After warming, the MPF activity of oocytes and their blastocyst formation and implantation rates in the CA, MG, and CA+MG groups were much higher than those in the untreated group. (molcells.org)
- 1998. Influence of organochlorine pesticides on maturation and post-fertilization development of bovine oocytes in vitro . (cdc.gov)
- This specialized division allows most maternal components to be maintained in the oocytes for early embryo development. (bioone.org)
Vitro8
- Potential of preimplantation genomic selection using the blastomere separation technique in bovine in vitro fertilized embryos. (researchmap.jp)
- Influence of organochlorine pesticides on development of mouse embryos in vitro . (cdc.gov)
- Since the development of in vitro embryo production in cattle, different supplements have been added to culture media to support embryo development, with serum being the most popular. (biomedcentral.com)
- Analysis of global gene expression in bovine embryos produced under different conditions can provide valuable information to optimize culture media for in vitro embryo production. (biomedcentral.com)
- We used RNA sequencing to examine the effect of in vitro embryo production, in either serum-containing or serum-free media, on the global gene expression pattern of individual bovine blastocysts. (biomedcentral.com)
- Importantly, in vitro production in the presence of serum appeared to have a different impact on the embryos according to their sex, with male embryos having three times more genes differentially expressed than their female counterparts (1283 vs. 456). (biomedcentral.com)
- Male embryos appeared to be most affected by suboptimal in vitro culture, i.e. in the presence of serum. (biomedcentral.com)
- Since the initial development of in vitro embryo production, the technique has been applied successfully to many species for clinical, commercial, and research purposes. (biomedcentral.com)
Murine1
- We previously demonstrated the critical involvement of SRC-2 in murine embryo implantation as well as in human endometrial stromal cell (HESC) decidualization, a cellular transformation process required for trophoblast invasion and ultimately placentation. (frontiersin.org)
Blastocysts2
- Test FITC-PIF uptake by cultured bovine blastocysts using fluorescent microscopy. (biomedcentral.com)
- FITC-PIF was taken-up by cultured bovine blastocysts, but not by scrambled FITC-PIF (control). (biomedcentral.com)
Uterus3
- Evidence that interferon-tau secreted from Day-7 embryo in vivo generates anti-inflammatory immune response in the bovine uterus. (researchmap.jp)
- Within the uterus, such signals support implantation [ 2 ] while in the periphery they induce and/or maintain tolerance without allowing for deleterious immune suppression to occur [ 3 ]. (biomedcentral.com)
- The fertilized conceptus enters the uterus as a 2- to 8-cell embryo and freely floats in the endometrial cavity about 90-150 hours, roughly 4-7 days after conception. (medscape.com)
Vivo4
- The chick chorio-allantoic membrane (CAM) assay is a commonly used method for studying angiogenic or anti-angiogenic activities in vivo . (biomedcentral.com)
- An in vivo assay to serve these purposes is the chick chorio-allantoic membrane (CAM) assay. (biomedcentral.com)
- Compared to in vivo derived embryos, embryos produced in serum-containing medium had five times more differentially expressed genes than embryos produced in serum-free conditions (1109 vs. 207). (biomedcentral.com)
- The transcriptome of embryos produced in serum-free conditions showed a greater resemblance to that of in vivo derived embryos, although genes involved in lipid metabolism were altered. (biomedcentral.com)
Maternal5
- After embryo aneuploidy, parental chromosomal translocations, maternal thrombophilic anomalies, immunological disorders, and obvious uterine ultrastructural abnormalities are excluded as etiologic contributors, implantation failure intrinsic to the endometrium is commonly suspected as an underlying cause of early pregnancy loss [EPL ( 1 )] and recurrent pregnancy loss [RPL ( 2 )], the latter defined as the loss of two or more consecutive pregnancies in the first trimester ( 2 - 5 ). (frontiersin.org)
- Soon after embryo attachment and the early stages of implantation, further invasion into the maternal compartment requires a functional decidua ( 6 ). (frontiersin.org)
- Since the immune milieu of pregnancy is unique - not replicated in any other circumstances - specific embryo-derived signals have a crucial role leading to maternal recognition of pregnancy [ 4 ]. (biomedcentral.com)
- To orchestrate such critical 'cross-talk', a viable embryo must be present, which may be accepted by the mother, whereas, a non-viable conceptus will fail to develop or later be rejected since maternal acceptance does not occur. (biomedcentral.com)
- This hormone is produced by the syncytiotrophoblast beginning on the day of implantation, and it rises in both the maternal blood stream and the maternal urine fairly quickly. (medscape.com)
Pregnancy4
- Our findings led us to conclude that a low level of MWirradiation-induced oxidative stress not only suppresses implantation, but it may alsolead to deformity of the embryo in case pregnancy continues. (nomedicalcare.com)
- PreImplantation Factor (PIF), a novel peptide secreted by viable embryos is essential for pregnancy: PIF modulates local immunity, promotes decidual pro-adhesion molecules and enhances trophoblast invasion. (biomedcentral.com)
- Furthermore, sperm DNA damage appears to be linked to the most important checkpoints of fertility such as reduced fertilization rates, lower embryo quality and pregnancy rates, higher miscarriage rates, malformations, and childhood diseases [ 22 ]. (hindawi.com)
- Evidence continues to suggest that ART does increase risk of higher order pregnancy (with its inherent pre- and perinatal risks), prematurity and low birth weight, congenital malformations, in particular of the male urogenital system, and imprinting disorders [ 23 - 26 ]. (hindawi.com)
Embryonic4
- A minority of mouse embryonic stem cells (ESCs) display totipotent features resembling 2-cell stage embryos and are known as 2-cell-like (2C-like) cells. (bvsalud.org)
- Efficient generation of embryonic stem cells from single blastomeres of cryopreserved mouse embryos in the presence of signalling modulators. (axonmedchem.com)
- Paternal sperm DNA damage has negative effects on the integrity of early embryonic development as the percentage of good quality embryos as well as implantation rates was significantly reduced in patients exhibiting high DNA damage [ 27 ] and it appears that the second and third mitoses are the sensitive periods [ 28 ]. (hindawi.com)
- Following germinal vesicle break down (GVBD) the oocyte is transcriptionally quiescent until fertilization and activation of the embryonic genome, occurring at the four-cell stage of development in the pig [ 9 ]. (biomedcentral.com)
Morula2
- PIF levels in mouse embryo culture medium significantly increased from the morula to the blastocyst stage (ANOVA, P = 0.01). (biomedcentral.com)
- Most embryos implant by the morula stage, when the embryo consists of many cells. (medscape.com)
Endometrium1
- The new embryo then induces the lining changes of the endometrium, which is called decidualization. (medscape.com)
ELISA4
- Determine PIF levels in culture medium of multiple mouse and single bovine embryos cultured up to the blastocyst stage using PIF-ELISA. (biomedcentral.com)
- PIF-ELISA use to assess IVF embryo quality prior to transfer is warranted. (biomedcentral.com)
- Overall, our data supports PIF's endogenous self sustaining role in embryo development and the utility of PIF- ELISA to detect viable embryos in a non-invasive manner. (biomedcentral.com)
- The enzyme-linked immunosorbent assay (ELISA) is the most popular in many clinical laboratories. (medscape.com)
Induce1
- However, the addition of serum during embryo culture can induce high birthweights and low viability in calves (Large Offspring Syndrome). (biomedcentral.com)
Developmental3
- Different developmental stages were profiled in Drosophila as well, an emphasis was placed on histone modification relevance. (wikipedia.org)
- Another drawback of oocyte vitrification is diminished developmental potential because of the decreased quality of embryos after warming. (molcells.org)
- Our study shows a developmental stage specific regulation of synemin isoforms in ES cells and its neural derivatives. (biomedcentral.com)
Serum9
- The serum concentrations of florfenicol were determined using microbiological assay with Bacillus subtilis (ATCC 25922) as a tested microorganism. (civilenvironjournal.com)
- The mean serum concentrations of florfenicol alone were markedly lower when compared with florfenicol pre-treated with vit E after single and multiple dosing. (civilenvironjournal.com)
- The peak serum concentrations (Cmax) were 5.9±0.46, 7.48±0.3 ug/ml, absorption half-life (t0.5ab) of 0.51±0.06, 0.71±0.1 h and elimination half-life (t0.5el) of 2.72±0.34, 3.34±0.5 after single florfenicol alone and florfenicol pre-treated with vit E respectively. (civilenvironjournal.com)
- Lipid peroxidation and antioxidant enzyme activities both in homogenate tissue and serum were assayed by colorimetric analysis. (biomedcentral.com)
- For example, embryos produced in serum-containing conditions had a lower expression of genes related to metabolism while embryos produced in serum-free conditions showed aberrations in genes involved in lipid metabolism. (biomedcentral.com)
- Serum supplementation had a major impact on the gene expression pattern of embryos, with male embryos being the most affected. (biomedcentral.com)
- In the early days, it was common practice to supplement culture media with serum to support embryo development in many species. (biomedcentral.com)
- Nevertheless, in many other aspects, the quality of the embryos produced in serum-free conditions is superior to that of embryos produced in the presence of serum. (biomedcentral.com)
- Furthermore, conventional MSC expansion with fetal bovine serum (FBS) causes immune- and inflammation-related problems following the exposure of xenogeneic pathogens from FBS [ 5 ]. (biomedcentral.com)
Viability3
- PIF is an early embryo viability marker that has a direct supportive role on embryo development in culture. (biomedcentral.com)
- The search to identify embryo-specific markers which reflect viability of cultured embryos by assessing the medium or by performing an embryo biopsy (beyond morphological evaluation) has been ongoing. (biomedcentral.com)
- Measurement of free radicals in the culture medium of developing embryos has been reported as an index for embryo viability. (biomedcentral.com)
Methods1
- Methods: Twenty male patients, ages ranged from 55 to 70 years, stage ΙΙ & ΙΙΙ according to modified Hoehn and Yahr (1997) classification of disabilities and ten male healthy elderly subjects, ages ranged from 55 to 70 years, participated in this study. (civilenvironjournal.com)
Post-fertilization1
- To determine the role of PIF in post-fertilization embryo development, we measured the peptide's concentration in the culture medium and tested endogenous PIF's potential trophic effects and direct interaction with the embryo. (biomedcentral.com)
Development4
- It is possible that H3R26me2 levels are changed before pre-implantation of bovine embryos and their development. (wikipedia.org)
- Revealing cell populations catching the early stages of the human embryo development in naïve pluripotent stem cells. (axonmedchem.com)
- Examine the inhibitory effects of anti-PIF-monoclonal antibody (mAb) added to medium on cultured mouse embryos development. (biomedcentral.com)
- The inability to transcribe mRNA during this stage of development and the probable necessity for post-transcriptional gene regulation (PTGR) suggests an important role for non-coding RNA in the maturing oocyte. (biomedcentral.com)
Significantly1
- We observed that implantation sites were affected significantly inMW-irradiated mice as compared to control. (nomedicalcare.com)
Genes1
- The selection of only a few genes to check embryo quality was based on the fact that the evaluation of expression of all the genes in the genome by qPCR was a daunting task. (biomedcentral.com)
Species1
- However, compared to other species, research on TE in livestock and poultry is still in its early stages. (biomedcentral.com)
Early1
- After implantation, self-renewal and proliferative cytotrophoblasts appear in the early first trimester (6-9 weeks) and can help identify trophoblast stem cells (TSCs) [ 13 ]. (biomedcentral.com)
19991
- 1999. Methoxychlor given in the pre- implantation period blocks sexual arousal in male mice. (cdc.gov)
Proliferative2
- Nicotine induced a reduction in the proliferative indexes of granulosa and theca cells in pre-antral and antral follicles ( P = 0.001). (biomedcentral.com)
- Co-Treatment with ginseng also reduced the expression of apoptotic markers and increased the proliferative indexes in granulosa and theca cells in pre-antral and antral follicles and also in stroma cells, in comparison to the nicotine group ( P = 0.001). (biomedcentral.com)
Spermatozoa1
- Thus, we investigated male mouse (m) PRL protein and mRNA expression in spermatozoa at various differentiation stages in the testes. (go.jp)
Single2
- In single bovine IVF-derived embryos, PIF levels in medium at day 3 of culture were higher than non-cleaving embryos (control) (P = 0.01) and at day 7 were higher than day 3 (P = 0.03). (biomedcentral.com)
- Total of 12 broiler chickens had been taken single and multiple oral administrations of florfenicol (30 mg/kg) alone or pre-treated with vit E (2 mg/kg). (civilenvironjournal.com)
Mouse1
- Detectable - though low - PIF levels were secreted already by 2-cell stage mouse embryos. (biomedcentral.com)
Human1
- Here we present the results of the screening of the human osteosarcoma cell lines HOS, MG63, MNNG-HOS, OST, SAOS, SJSA1, U2OS and ZK58 for their use in the CAM assay and provide evidence that the MNNG-HOS cell line reproducibly simulates key features of human osteosarcoma growth. (biomedcentral.com)
Mice2
- At the end of a total of 45 days of exposure, mice weresacrificed, implantation sites were monitored, blood was processed to study stress parame-ters (hemoglobin, RBC and WBC count, and neutrophil/lymphocyte (N/L) ratio), the brainwas processed for comet assay, and plasma was used for nitric oxide (NO), progesterone andestradiol estimation. (nomedicalcare.com)
- We also suggest that MWradiation-induced oxidative stress by increasing ROS production in the body may lead toDNA strand breakage in the brain cells and implantation failure/resorption or abnormalpregnancy in mice. (nomedicalcare.com)
Activity2
- Osteogenic effects of TSC-EVs on MSCs were assessed by alkaline phosphatase (ALP) activity, calcium assays, and calvarial bone defect animal models. (biomedcentral.com)
- Oocyte arrest at the diplotene stage is maintained in part through the activity of phosphodiesterase enzymes that catalyze the hydrolysis of cyclic adenosine monophosphate (cAMP) into AMP [ 1 , 2 ]. (biomedcentral.com)
Meiotic1
- The oocyte must maintain arrest at the diplotene stage until meiotic resumption occurs. (biomedcentral.com)
Medium2
- In contrast, atretic embryos medium was similar to the medium only control. (biomedcentral.com)
- In non-cleaving embryos culture medium was similar to medium alone (control). (biomedcentral.com)
Results1
- Our results show that the CAM assay is a useful tool for studying osteosarcoma growth. (biomedcentral.com)
Multiple1
- While, after multiple dosing, (Cmax) were 7.4±0.3, 8.04±0.3 ug/ml, (t0.5ab) 0.82±0.04, 0.81±0.04 h and (t0.5 el) 3.77±0.2, 4.52±0.7 h after multiple dosing of florfenicol alone and florfenicol pre-treated with vit E respectively. (civilenvironjournal.com)
Support1
- While host nerve integration and formation of NMJs were evident within the implanted skeletal muscle constructs, we observed that the constructs did not support the full restoration of defected muscles at 8-week post-implantation, which is likely due to the delayed integration of host nerve. (nature.com)
Models1
- The model provides an excellent alternative to current rodent models and could serve as a preclinical screening assay for anticancer molecules. (biomedcentral.com)
Failure1
- Implantation failure Microwaves (MW) are non-ionizing electromagnetic radiation (EMR) (wavelength rangingfrom 1 mm to 1 m and frequency between 0.3 and 300 GHz), which unlike ionizingradiation, do not contain sufficient energy to break the bond or chemically change thesubstances by ionization. (nomedicalcare.com)
Determine1
- We employed cell proliferation analyses such as CCK8 and BrdU assays to determine the proliferation-promoting role of TSC-EVs on MSCs. (biomedcentral.com)
Major1
- The CAM assay is characterized by several major advantages such as the ease of access, the extensive vascularisation and the relatively simple experimental approach. (biomedcentral.com)