Assay apoptotic. Medical search. Frequent questions

The number of live RGCs was then counted using a cell viability assay. (molvis.org)
Learn about cell viability assays. (abcam.com)
Cell viability was determined by using the MTT assay and the morphological changes were observed. (researchgate.net)
Cell viability was analyzed using 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2h-tetrazolium bromide assay. (molvis.org)
The anti-proliferative effect of A. cherimola ethanolic leaf extract was evaluated via cell viability assay. (nih.gov)
A cell viability assay demonstrated that SP2 could significantly enhance the viability of HUVEC. (hindawi.com)
Cell viability and cytotoxicity assays are used usually for screening of drugs and cytotoxicity tests of chemicals. (benthamscience.com)
Replication competence was found to correlate well with viability results from the acridine orange / ethidium bromide assay, but not with results from the trypan blue assay. (mit.edu)
Fipronil mediated activation of caspase-3/7 in concurrence with compromised ATP production and viability are attributed to apoptotic cell death. (cdc.gov)

BD Biosciences provides you a choice of flow cytometry-based kits for the detection of early apoptotic cells. (bdbiosciences.com)
These experiments proved that early apoptotic cells, which have lost membrane integrity but not chromatin integrity, can be considered dead since they lose the ability to replicate. (mit.edu)

Apoptotic peak was detected by flow cytometry and the ladder band appeared in DNA electrophoresis. (researchgate.net)

Since EGCG + Zn2+ may, at least in part, protect cardiac myocytes against H/R‑induced apoptotic cell death, the PI3K/Akt pathway of EGCG may be enhanced by its interactions with zinc during H/R injury. (spandidos-publications.com)
In addition, Townsend et al ( 12 ) reported that EGCG reduced signal transducers and activators of transcription-1 phosphorylation and protected cardiac myocytes against I/R-induced apoptotic cell death in isolated rat hearts. (spandidos-publications.com)

The trypan blue assay indicated that cytotoxic effects contributing to an increase of greater than 10% of control values was indicated at doses above 12.5 µM. (cdc.gov)

An introductionto the mechanisms of apoptotic cell death and apoptosis assays and markers. (abcam.com)

There was also no translocation of apoptosis-inducing factor (AIF) from mitochondria to the nucleus in apoptotic keratinocytes, indicating Tan IIA-induced apoptosis occurs mainly through the caspase pathway. (hindawi.com)
Protein and gene expression of apoptotic signaling pathway-related proteins such as caspase-3, cytochrome c, and PARP were determined to provide evidence for the mechanisms of action of Tan IIA in the treatment of psoriasis. (hindawi.com)
Furthermore, administration of EGCG in vitro was observed to prevent apoptosis of cardiomyocytes by regulating pro-apoptotic and anti-apoptotic proteins, including B cell lymphoma-2 (Bcl-2) and Bcl-2-associated X protein, and by simultaneously regulating caspase-3 in isolated rat hearts ( 13 ). (spandidos-publications.com)
Non-cytotoxic assay arrests further apoptotic activity via caspase inhibition. (immunochemistry.com)
Useful tips for selecting caspase substrate-based activity assays. (abcam.com)
Discover pathways on apoptosis induction, caspase activation and phagocytosis of apoptotic cells. (abcam.com)
Identification and quantification of caspase-3 activity in apoptotic cells. (bdbiosciences.com)
BD Biosciences provides a variety of reagents for the identification and quantification of caspase-3 activity in apoptotic cells. (bdbiosciences.com)

Apoptotic cells undergo several physiological processes, including changes in cell membrane permeability and chromatin condensation, which can be distinguished using different dyes in apoptotic and necrotic cell staining protocols. (thermofisher.com)
The purpose of this study was to investigate whether astaxanthin confers a neuroprotective effect against glutamate stress, oxidative stress, and hypoxia-induced apoptotic or necrotic cell death in primary cultures of rat retinal ganglion cells (RGCs). (molvis.org)
Our results suggest that astaxanthin has a neuroprotective effect against RGC death induced by glutamate stress, oxidative stress, and hypoxia, which induce apoptotic and necrotic cell death. (molvis.org)

Learn about the different types of cytotoxicity assay. (abcam.com)
The cytotoxicity of etoposide and VPA to a UKF-NB-4 neuroblastoma cell line was evaluated with MTT assay. (nel.edu)
A cytotoxicity study was conducted on two human cancer cell lines, pancreatic and ovarian, using sulforhodamine B assay. (ijpsonline.com)

All the data presented in our study show that the terpene-rich A. cherimola ethanolic leaf extract exhibits an anti-proliferative and pro-apoptotic effect on the AML cell lines used. (nih.gov)
Anti-proliferative activity and apoptotic effect of tick salivary gland extracts on human HeLa cells. (nel.edu)
The anti-proliferative and apoptotic potential of salivary gland extracts (SGE) of unfed and partially fed adult ixodid ticks were screened using human HeLa cells. (nel.edu)
Kazimírová M, Dovinová I, Rolníková T, Tóthová L, Hunáková L. Anti-proliferative activity and apoptotic effect of tick salivary gland extracts on human HeLa cells. (nel.edu)

Learn about the assays used to assess cell proliferation and the cell cycle. (abcam.com)
To further investigate the molecular mechanism by which the extract promoted apoptosis and inhibited the proliferation of the AML cells used, apoptotic protein expression was determined through western blots. (nih.gov)
Choose from a variety of BD Biosciences assays and reagents for visualizing cellular events such as apoptosis, cell proliferation and the cell cycle. (bdbiosciences.com)
Cell proliferation was tested by MTT, while invasion was detected in Transwell chamber assay. (medscimonit.com)
Cell proliferation was detected by MTT assay and apoptosis was measured by FACS in ESCC cells after WISP2 downregulation and overexpression. (biomedcentral.com)
Peripheral blood mononuclear cells (PBMC) were isolated and the level and function of Treg cells was analyzed by flow cytometry and cell proliferation assays. (fluoridealert.org)
ABSTRACT This study examined haematopoietic stem cells of 19 high-risk cases of myelodysplastic syndrome (MDS) for apoptotic and anti-apoptotic signals and cellular proliferation and correlated these with clinical and cytogenetic subtypes, particularly trisomy 8. (who.int)

Putative CSCs were isolated from human melanoma cells of a different aggressiveness, Hs294T and A375 cell lines, grown under hypoxia using "sphere-forming assay", CD133 surface expression and migration ability. (oncotarget.com)

Vybrant Apoptosis Assay kits offer streptavidin biotin-annexin V, propidium iodide (PI), YO-PRO-1, and Hoechst 33342 dyes to differentiate live from apoptotic or necrotic cells, which can then be visualized using flow cytometry. (thermofisher.com)

We recently discovered a new membrane protrusion (termed beaded apoptopodia) generated by apoptotic monocytes which fragments to release an abundance of ApoBDs. (nature.com)
To investigate the function of apoptotic monocyte disassembly, we used influenza A virus (IAV) infection as a proof-of-concept model, as IAV commonly infects monocytes in physiological settings. (nature.com)
Apoptotic monocytes generate long membrane protrusions that exhibit a unique 'beads-on-a-string' morphology, termed beaded apoptopodia, which undergo a segmentation-like event to fragment into an abundance of ApoBDs. (nature.com)
Therefore, we utilised IAV infection as a model to further dissect the functional consequence of the disassembly of apoptotic monocytes. (nature.com)
In addition, a negative correlation was detected between the F urinary levels and the proportion of apoptotic cells, in PBMC or T cells or monocytes (P? (fluoridealert.org)
Flow cytometric estimation of the apoptotic marker CD95 in peripheral neutrophils, lymphocytes and monocytes was done for 18 infants with non-oedematous protein energy malnutrition (PEM) and 12 oedematous ones, on hospital admission and after supervised nutritional rehabilitation, and compared with 12 matched controls. (who.int)

Cell migration and invasion were analyzed by wound healing assay and transwell migration assay, respectively. (biomedcentral.com)

Caspases are intracellular proteases that are suppressed by Inhibitor of Apoptosis Proteins (IAPs), a family of evolutionarily conserved anti-apoptotic proteins. (bindingdb.org)
Caspases, a class of proteins found to be universally important in inducing apoptosis, were found to be activated in apoptotic CHO cells. (mit.edu)

6 In addition, independent annexin V/Hoechst assays of primary T-PLL cells on NK-Tert stromal support demonstrated modest single-agent activity of ibrutinib ( Figure 1D , Online Supplementary Figure S2A ). (haematologica.org)

Apoptotic cells undergo changes including cell shrinkage and chromosomal DNA fragmentation. (abcam.com)

Understand the methods used for TUNEL staining (also known as the TUNEL assay), and review TUNEL staining kits. (abcam.com)
Using a fluorescein apoptosis detection system (Promega G3250), a TdT-mediated dUTP nick-end labeling (TUNEL) assay was performed on both planchets and slides. (cdc.gov)
We attempted to do the reverse procedure, with the silica localization first, then the TUNEL assay. (cdc.gov)

Histologic examination reveals variable epidermal atrophy, basal vacuolar degeneration, apoptotic or dyskeratotic keratinocytes, and lymphocytic interface dermatitis (see the images below). (medscape.com)

BD Biosciences offers a complete suite of assays and reagents for visualizing key cellular events. (bdbiosciences.com)
Taseen Gul, Henah Mehraj Balkhi, Ehtishamul Haq , " Evaluation of Cellular Processes by In Vitro Assays ", Bentham Science Publishers (2018). (benthamscience.com)

Cell growth inhibition activity of SGE was measured by the MTT assay. (nel.edu)
Cells deprived of steroid hormones were exposed to increasing concentrations (1-100 μM) of pomegranate compounds in the presence of 0.1 nM dihydrotestosterone (DHT), and the inhibition of cell growth was measured by WST-1 colorimetric assay after a 4 day exposure. (nih.gov)

Apoptotic cells can undergo a series of morphological changes to aid cell fragmentation, a process termed apoptotic cell disassembly 4 . (nature.com)
Furthermore, Tan IIA-induced apoptosis and mitochondrial membrane potential changes were also further demonstrated by DNA fragmentation, single-cell gel electrophoresis assay (SCGE), and flow cytometry methods. (hindawi.com)

Apoptotic was detected by morphological changes, DNA electrophoresis and flow cytometry. (researchgate.net)

specific localization of apoptotic nuclei was apparent only outside the scanned regions. (cdc.gov)

Transient receptor potential (TRP) channels contribute to the regulation of intracellular calcium, which can promote cancer hallmarks in cases of dysregulation of gene transcription and calcium-dependent pro-proliferative or anti-apoptotic mechanisms. (dovepress.com)
In conclusion, punicic acid, the main constituent of pomegranate seed (70-80%), exhibited potent growth inhibitory activities in androgen-dependent LNCaP cells, which appear to be mediated by both antiandrogenic and pro-apoptotic mechanisms. (nih.gov)

It was demonstrated that when EGCG interacted with zinc, the anti‑apoptotic activity was significantly enhanced. (spandidos-publications.com)
In contrast, expression of bcl- 2, a well-characterized anti-apoptotic gene, was able to significantly increase the life of CHO batch cultures in response to both glucose limitation and growth factor withdrawal. (mit.edu)
Immunohistochemical examinations of colon tissues showed significantly lower neutrophil counts, more granzyme B-positive but fewer MMP9 (gelatinase B)-positive cancer cells and lower numbers of apoptotic cells in mice receiving AAT therapy. (lu.se)
High-risk MDS cases had a significantly higher percentage of apoptotic CD34+ cells and anti-apoptotic survivin+ cells than controls, particularly for trisomy 8 cases. (who.int)

We aim to identify further molecules involved in the activation of complement in joints in RA with the goal of developing diagnostic assays for diagnosis, prognosis and follow up of treatment. (lu.se)
An Apoptotic Gene Signature for the Prognosis of Hepatocellular Carcinoma. (cdc.gov)

The apoptotic activity of RGCs under glutamate stress increased to 32%, but was reduced to 15% with 100 nM astaxanthin administration. (molvis.org)
Applying this concept in a screening assay, we identified a compound targeting the TMD of death receptor p75 NTR that induced profound conformational changes and receptor activity. (rcsb.org)
The compound triggered apoptotic cell death dependent on p75 NTR and JNK activity in neurons and melanoma cells, and inhibited tumor growth in a melanoma mouse model. (rcsb.org)
Confirmation of bDNA results were sought through western blotting and/or enzyme activity assays. (cdc.gov)

Effects of this therapy were evaluated histologically, and by immunohistochemical and gene expression assays. (lu.se)

These FAM-peptide-FMK compounds are both cell permeable and non-cytotoxic during the course of the assay and thus allow the detection of active caspases in living cell systems. (immunochemistry.com)
Improved Variant Detection in Clinical Myeloid NGS Testing by Supplementing a Commercial Myeloid NGS Assay with Custom or Extended Data Filtering and Accessory Fragment Analysis. (cdc.gov)

Putative CSCs are isolated using methods based on several in vitro/in vivo assays, such as expression of distinct surface markers or intracellular enzyme activities, "sphere-forming" ability in non-adherent culture and/or initiation of new tumor growth when xenotransplanted into immunodeficient mice [ 8 ]. (oncotarget.com)

IAV was able to induce monocyte apoptosis and apoptotic cell disassembly in both in vitro and in vivo settings. (nature.com)

AgNPs were toxic to the cells, which can lead to damage of deoxyribonucleic acid, generation of reactive oxygen species, mitochondrial damage and apoptotic induction[ 7 , 8 ]. (ijpsonline.com)

Furthermore, both ELISA and flow cytometry results indicated that SP2 has anti-apoptotic effects on staurosporine-induced HUVEC apoptosis. (hindawi.com)
Flow cytometry based assays and kits for detecting apoptosis. (bdbiosciences.com)
The BD ® Calcium Assay Kits enable the measurement of intracellular calcium changes caused by activation of G-protein coupled receptors or calcium channels, quickly and easily, by flow cytometry. (bdbiosciences.com)

In addition, the expressed protein in the silkworm baculovirus system was used to study the anti-apoptotic effects of SP2 on human umbilical vein ECs (HUVECs) induced by peroxidation. (hindawi.com)
Besides, S63A and/or S262A of IκBα regulated apoptotic and necroptic effects in breast cancer cells. (researchsquare.com)
In this study, we investigated the growth inhibitory, antiandrogenic, and pro-apoptotic effects of 13 pure compounds found in the pomegranate in androgen-dependent LNCaP human prostate cancer cells. (nih.gov)
Cytotoxic effects of fipronil and fipronil sulfone were examined using the adenylate kinase and the trypan blue exclusion assays in HepG2 cells and human hepatocytes. (cdc.gov)

Annexin V-FITC was used to detect apoptotic cells. (nel.edu)

Western blot analysis revealed a Bax/Bcl2 dependent mechanism of apoptosis, as well as PARP cleavage, confirming the apoptotic results observed previously. (nih.gov)
Our results suggest that although trisomy 8 cells are in a pro-apoptotic state, they are checked by the enhanced expression of anti-apoptotic signals which provide them with their proliferative advantage. (who.int)

Together, this study reveals a previously unrecognised function of apoptotic monocyte disassembly in the pathogenesis of IAV infections. (nature.com)
Plaque assays have been used to clonally segregate laboratory-adapted C. trachomatis strains from mixed infections, but no assays have been reported to segregate clones from recent clinical samples. (cdc.gov)

Review our assay kits for the study of apoptosis. (abcam.com)

We developed a novel shotgun cell culture harvest assay for this purpose because we found that recent clinical samples do not form plaques. (cdc.gov)

Exposure of human hepatocytes to doses of fipronil (5-amino-1-[2,6-dichloro-4-(trifluoromethyl)phenyl]-4-[(trifluoromethyl) sulfinyl]-1H-pyrazole-3-carbonitrile) ranging from 0.1 to 25 µM resulted in a dose dependent increase in CYP1A1 mRNA expression (3.5 to approximately 55-fold) as measured by the branched DNA assay. (cdc.gov)

Its pro-apoptotic effect was assessed through Cell Death ELISA and dual Annexin V/PI staining. (nih.gov)

In this way we were able to determine that silica was localized in lipoprotein- and macrophage-rich aggregates in the alveoli, and that the apoptotic cells were found in these same regions. (cdc.gov)

Using the images as a guide, each of 6-8 apoptotic regions was located on the planchet under a Leica MZ12 gross microscope. (cdc.gov)

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