Aspartic AcidAmino AcidsAmino Acid SequenceAspartic Acid ProteasesSequence Homology, Amino AcidAspartic Acid EndopeptidasesAmino Acid SubstitutionMolecular Sequence DataCloning, MolecularMutagenesis, Site-DirectedBase SequenceBinding SitesMutationEscherichia coliSequence AlignmentAsparagineKineticsModels, MolecularProtein ConformationRecombinant ProteinsStructure-Activity RelationshipSubstrate SpecificityPeptide FragmentsAmino Acids, EssentialAmino Acid MotifsPeptidesProtein Structure, TertiaryAmino Acid Transport SystemsProtein BindingAlanineDNA, ComplementaryPepstatinsMolecular WeightSequence Homology, Nucleic AcidGlycineCell LineBacterial ProteinsElectrophoresis, Polyacrylamide GelProtein Structure, SecondaryPoint MutationDNAConserved SequenceLeucineSerineHistidineHydrogen-Ion ConcentrationPlasmidsGlutamatesLysinePepsin ARecombinant Fusion ProteinsCatalysisRestriction MappingChromatography, High Pressure LiquidTrypsinEndopeptidasesDNA PrimersIsoaspartic AcidCathepsin EProteinsAmino Acids, AromaticGlutamic AcidCattleSequence Analysis, DNAArginineAmino Acids, Branched-ChainCodonThreoninePhylogenyAmino Acids, SulfurTransfectionProlineMutagenesisRNA, MessengerHydrolysisGenes, BacterialSpecies SpecificityPolymerase Chain ReactionOligopeptidesCatalytic DomainCyanogen BromideGlutamineCarrier ProteinsCysteineSaccharomyces cerevisiaeIsoleucineCarbohydratesChromatography, GelMembrane ProteinsProtein Processing, Post-TranslationalPhosphorylationValineMacromolecular SubstancesCOS CellsPhenylalanineMethionineTryptophanGenesProtein PrecursorsSequence Analysis