• Achaete-scute complex homolog 2 (Drosophila), also known as ASCL2, is an imprinted human gene. (wikipedia.org)
  • Achaete-scute complex homolog 2 (ASCL2) is a maternally expressed imprinted gene that codes is a part of the basic helix-loop-helix (BHLH) transcription factor family. (wikipedia.org)
  • To investigate the role of achaete-scute complex-like 2 (ASCL2) in stomach adenocarcinoma (STAD), we analyze whether ASCL2 suppression could retard cancer development and further observe the relevance between ASCL2 and inflammation via Toll-like receptor 4 (TLR4) activation in STAD, both in vitro and in vivo. (biomedcentral.com)
  • Achaete-scute complex-like 2 (ASCL2) is a member of the basic helix-loop-helix family of transcription factors, binds to the E-box (5′-CANNTG-3′), and stimulates the transcription [ 8 ]. (biomedcentral.com)
  • 0.05) decreased the expression of trophinin 2 (Trop2), leucine-rich repeat containing G protein-coupled receptor 5 positive (Lgr5+), male-specific lethal 1(Msl1), BMI 1 proto-oncogene, polycomb ring finger (Bmi1), and achaete-scute family bHLH transcription factor 2 (Ascl2). (ruifufeed.com)
  • In order to identify prognostic master regulators, we took the known 85 prognostic signature genes for colorectal cancer and inferred their upstream TFs. (biomedcentral.com)
  • In colorectal cancer, an early mutation leading to such features usually occurs in the APC or CTNNB1 genes, thereby activating Wnt signalling. (biomedcentral.com)
  • Further, ASCL2 knockdown altered the expression of BIRC5 and CD44, which are Wnt-associated genes. (wikipedia.org)
  • The study aims to investigate the potential role and function of ASCL2 in STAD, then apply ASCL2 knockdown lentivirus (sh-ASCL2) to enhance apoptosis, inflammatory progress, and tumor development in vivo and in vitro. (biomedcentral.com)
  • Significantly differentially expressed (SDE) immunological genes and transcription factor (TF) were selected for functional pathways and transcriptional signaling identification. (frontiersin.org)
  • The distal region of mouse chromosome 7 (Chr 7) contains at least ten imprinted genes, several of which are expressed from the maternal homologue in the placenta. (biomedcentral.com)
  • Taken together, our results demonstrate that all the distal chromosome 7 imprinted genes implicated in placental function are silenced by IC2 and Kcnq1ot1 on the paternal allele. (biomedcentral.com)
  • See also {614327} for a tumor predisposition syndrome that may contribute to the development of malignant mesothelioma upon asbestos exposure and is caused by germline mutation in the BAP1 gene ( OMIM ) on chromosome 3p21. (mendelian.co)
  • Human ASCL2 genome location and ASCL2 gene details page in the UCSC Genome Browser. (wikipedia.org)
  • With advances in genome-wide gene expression technologies, classification of cancer subtypes based on expression signatures is widespread and results in many biomarkers for various cancers. (biomedcentral.com)
  • However, it is unknown whether some maternally expressed genes are silenced on the paternal homologue via a Kcnq1ot1- independent mechanism. (biomedcentral.com)
  • 4) RT-PCR profiler Arrays using stem cell (SC) panel of 84 genes for four group of cells (1) CD44 + /CD24 - /EpCAM - BCSCs, (2) CD44 + /CD24 - /EpCAM + BCSCs, (3) mammospheres, and (4) normal breast tissues. (harvard.edu)
  • ASCL2 expression was noted in multiple breast cancer cell lines, most notably in MCF7 cells. (wikipedia.org)
  • Notably, these TFs were selected solely from inferred regulatory links using gene expression profiles and included many TFs regulating tumorigenic processes such as proliferation, metastasis, and differentiation. (biomedcentral.com)
  • Our network approach leads to the identification of the upstream transcription factors for prognostic signature genes to provide leads to their regulatory mechanisms. (biomedcentral.com)
  • We demonstrate that our approach could identify upstream biomarkers for a given set of signature genes with markedly smaller size and comparable performances. (biomedcentral.com)
  • ASCL2 is possibly an upstream target factor of the TLR4 signaling pathway. (biomedcentral.com)
  • Nevertheless, in stomach carcinoma, the potential role of ASCL2 in inflammation via TLR4 activation or its pathways has not been completely clarified. (biomedcentral.com)
  • What pathways are this gene/protein implicaed in? (cancer-genetics.org)
  • A total of 7,928 SDE genes and 46 canonical pathways derived from it were identified. (frontiersin.org)
  • Twenty-four potential transcriptional regulatory pathways were identified based on SDE TFs matched with their corresponding SDE immunological genes. (frontiersin.org)
  • The Mash2/Ascl2 transcription factor is required for normal placental development. (geneimprint.com)
  • Loss of function of ASCL2 will result in embryonic failure. (wikipedia.org)
  • The corresponding paternal alleles of these genes are silenced in cis by an incompletely understood mechanism involving the formation of a repressive nuclear compartment mediated by the long non-coding RNA Kcnq1ot1 initiated from imprinting centre 2 (IC2). (biomedcentral.com)
  • In the last decade, diagnostic and prognostic evaluation has been facilitated by global gene expression profiling (GEP), provid- ing a new powerful means for the classification, prediction of survival, and response to treatment of lym- phomas. (lu.se)
  • Gene expression signatures have been commonly used as diagnostic and prognostic markers for cancer subtyping. (biomedcentral.com)
  • The selected TFs showed a comparable or slightly better prognostic performance than the original 85 signature genes in spite of greatly reduced number of marker genes from 85 to 10. (biomedcentral.com)
  • ASCL2 is particularly important during implantation of the developing embryo, specifically in placental development and neuronal precursor determination. (wikipedia.org)
  • However, the remaining genes in the cluster ( Ascl2 , Phlda2 , and Cdkn1c ) have well-documented roles in placentation since knockouts of each of these genes result in drastic placental phenotypes. (biomedcentral.com)
  • The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. (cancer-genetics.org)
  • Our CRISPR screens exposed other unique dependencies in POU2F3-expressing SCLC lines, including the lineage TFs SOX9 and ASCL2 and the receptor tyrosine kinase IGF1R (insulin-like growth factor 1 receptor). (cshl.edu)
  • The mammospheres showed significant downregulation in 9 genes and a significant upregulation in 35 genes. (harvard.edu)
  • In reaction to Wnt activation we observe downregulation of location specific genes and differentiation markers. (biomedcentral.com)
  • In 2010, Tian et al reported that ASCL2's involvement in metastasis could be attributed by ASCL2-promoted cellular self-renewal as opposed to cellular differentiation. (wikipedia.org)
  • Transcription factors (TFs) are critical for B-cell differentiation, affecting gene expression both by repres- sion and transcriptional activation. (lu.se)
  • In 2006, ASCL2 was observed to be involved in tumor progression, specifically, ASCL2 was reported to be upregulated in colorectal tumors (Jubb et al). (wikipedia.org)
  • P = 0.003) in the Iron Reactive Element/Iron Regulatory Protein (IRE/IRP) signaling pathway although iron and the interaction of VA and iron had no effect on the genes' expression. (ruifufeed.com)
  • Western blotting of ASCL2 with the target protein of immune-associated cells is performed. (biomedcentral.com)
  • This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. (cancer-genetics.org)
  • What does this gene/protein do? (cancer-genetics.org)
  • The potential bio-imprisonment of transgen, high protein expression and the possibility of regulating genes into operons representing a considerable profit that makes valuable target chloroplast in agricultural biotechnology. (gentaur-biotech.com)
  • Search the gene expression profiles from curated DataSets in the Gene Expression Omnibus (GEO) repository. (cancer-genetics.org)
  • applied a reverse engineering method for context-specific transcriptional regulatory networks to 176 gene expression profiles from high-grade glioblastoma (HGG) patients. (biomedcentral.com)
  • In 2019, Farmarzi reported a potential link between ASCL2 and breast cancer tumorigenesis which had never previously been demonstrated. (wikipedia.org)
  • Without Ascl2, the GlyT cells are not found and even though these cells develop later in gestation, its progenitor cells are established early on. (wikipedia.org)
  • siRNA KO of ASCL2 was shown to inhibit cellular migration of breast cancer cells. (wikipedia.org)
  • Selumetinib causes long-term G1 arrest accompanied by reduced expression of DNA replication and repair genes, but cells stochastically re-enter the cell cycle during treatment despite continued repression of pERK1/2. (babraham.ac.uk)
  • ASCL2 expression is high in MGC803 cells. (biomedcentral.com)
  • The ASCL2 may adjust inflammation in STAD through TLR4 activation and may be associated with related immune cells. (biomedcentral.com)
  • During research projects under the supervision of Prof. Dieter Gruenert in San Francisco, USA and Prof. William James in Oxford, UK, he worked on gene therapeutic approaches utilizing CRISPR/Cas9 technology in induced pluripotent stem cells (iPSCs). (sendoellab.org)
  • Genes up-regulated in comparison of CD4 [GeneID=920] dendritic cells (DC) versus CD4- [GeneID=920] CD8- DCs. (gsea-msigdb.org)
  • We used a global gene profiling approach to determine gene expression patterns among murine splenic CD11c high DC subsets in an effort to better characterise these cells. (gsea-msigdb.org)
  • Furthermore, we identified subgroup-specific drivers of medulloblastoma using functional enrichment analysis, Shapley values, and gene network analysis. (neurosurgery.directory)
  • Mutations and gene amplifications that confer drug resistance emerge frequently during chemotherapy, but their mechanism and timing are poorly understood. (babraham.ac.uk)
  • Genomic imprinting is the mechanism by which haploid maternal and paternal genomes carry different epigenetic marks, resulting in monoallelic transcription of a subset of genes which are expressed exclusively from either the maternal or paternal allele [ 1 ]. (biomedcentral.com)
  • No visible phenotype was observed upon paternal or maternal transmission of the deletion, which encompasses the Th gene. (geneimprint.com)
  • The location specific Wnt signatures are dominated by genes which have been lowly expressed in the tissue of origin, and are the targets of transcription factors that are activated following enhanced Wnt signalling. (biomedcentral.com)
  • To address this issue, an AI-based R package called MBMethPred was developed based on DNA methylation and gene expression profiles of 763 medulloblastoma samples to classify subgroups using machine learning and neural network models. (neurosurgery.directory)
  • RNA-seq was performed in blood Ly6C high and Ly6C low MC sorted by flow cytometry from control and HHcy cystathionine β-synthase gene-deficient ( Cbs -/- ) mice. (frontiersin.org)
  • Insufficient Ascl2 function is also associated with a placenta that has phenotypic defects, which leads to growth retardation. (wikipedia.org)
  • Specific RNA inhibitors, miRNA mimics, and small interfering RNA (siRNA) were used to evaluate the role of a candidate miRNA and its target genes in P-MSC-EV-induced improvements in the function of HG-induced senescent HDFs. (bvsalud.org)
  • We selected the top 10 TFs as candidate master regulators to show the highest coverage of the signature genes among the total 846 TF-target sub-networks or regulons. (biomedcentral.com)
  • Furthermore, the signature of Wnt target genes differs between the three intestinal locations in the organoids. (biomedcentral.com)
  • Polymorphisms in the CD58 gene are associated with increased risk for multiple sclerosis . (wikidoc.org)
  • Furthermore, our results demonstrate that the methylated maternal IC2 is not required for the regulation of nearby genes. (biomedcentral.com)
  • Our findings demonstrate that acquisition of MEK inhibitor resistance often occurs through gene amplification and can be suppressed by impeding cell cycle entry in drug. (babraham.ac.uk)
  • Proliferation, development, inflammation, and apoptosis in STAD are observed using sh-ASCL2 lentivirus via TLR4 activation in vitro and in vivo. (biomedcentral.com)
  • Sh-ASCL2 could reduce STAD development by decreasing proliferation, tumor volume, and biomarker levels and increasing apoptosis in vitro and in vivo. (biomedcentral.com)
  • In particular, the genes involved in the nervous system development process have the potential to separate medulloblastoma subgroups with 99% accuracy. (neurosurgery.directory)
  • Ascl2 plays a critical role in early gestation, with its products showing up in the oocyte and the two-cell stage of the zygote. (wikipedia.org)
  • This gene has its primary role after implantation of the developing embryo. (wikipedia.org)
  • The inflammatory role of ASCL2 is regulated through TLR4 activation. (biomedcentral.com)
  • however, the mechanism underlying the role of the ASCL2 in inflammation in stomach carcinoma has not been explained. (biomedcentral.com)
  • Imprinted expression of several of the IC2-regulated genes critical to placentation is also faithfully recapitulated in DelTel7/IC2KO placentae. (biomedcentral.com)
  • Then, the regulatory TFs for the mesenchymal signature genes were inferred from the use of master regulator analysis (MRA) together with or without stepwise linear regression method (SLR). (biomedcentral.com)
  • To validate and generalize our findings, we use gene expression data from patients. (biomedcentral.com)
  • A similar effect is seen in patient data, where genes with significant differential expression between the normal left and right colon are downregulated in the cancer samples. (biomedcentral.com)
  • RGD-PIPELINE: ftp-file-extracts # MODULE: orthologs-2-version-2022-08-26 # GENERATED-ON: 2023/08/19 # RGD Ortholog FTP file for CHINCHILLA # From: RGD # URL: http://rgd.mcw.edu # Contact: [email protected] # # Format: # Comment lines begin with '#' # Tab delimited fields, one line per CHINCHILLA gene. (mcw.edu)
  • The reason for this is that passengers frequently take the majority of the signature gene and an accurate discrimination of cancer drivers from passengers becomes a key subject in cancer genomic studies. (biomedcentral.com)
  • The ASCL2 shows a negative correlation with inflammation, and TLR4 reveals a positive correlation with gastric cancer. (biomedcentral.com)
  • Chronic gastritis is due to an infection of Helicobacter pylori ( H. pylori ), and it has been reported that inflammatory response-related genes are linked to the etiology of gastric cancer [ 2 ]. (biomedcentral.com)
  • This gene has also been linked to other cancers, including androgen-independent prostate cancer. (cancer-genetics.org)
  • Considering the important functions of several IC2-regulated genes in placentation, we set out to determine whether these DelTel7/IC2KO rescued conceptuses develop normal placentae. (biomedcentral.com)