Articular scaffold for tissue engineering tissue engineering. Medical search. Frequent questions

Research proposed a number of experimental approaches encompassing all the typical strategies of regenerative medicine: cell-free scaffolds, gene therapy, intra-articular delivery of progenitor cells, biological glues for enhanced bonding of reparable tears, partial and total tissue engineered meniscus replacement. (unibas.ch)
Intra-articular Injection of Urinary Bladder Matrix Reduces Osteoarthritis Development. (ndriresource.org)
The obtained cell suspension can be used directly as an intra-articular or intra-tissue injection. (educell.si)
In 1918, Smith detailed combined intra-articular and extra-articular procedures. (medscape.com)
The ligament is intra-articular but extrasynovial. (medscape.com)

In this study, we showed that this structure enabled the uniform seeding of goat chondrocytes in the scaffolds, and with low rates of leakage. (elsevierpure.com)
In the present chapter, we report the development of heparin-, chondroitin sulfate-, decorin-, and poly(ε-caprolactone)-based self-assembling peptide composite scaffolds to promote re-differentiation of expanded human articular chondrocytes and induction of adipose-derived stem cells to chondrogenic commitment. (intechopen.com)
Articular cartilage is mainly composed of chondrocytes and dense extracellular matrix (ECM) without blood vessels or innervation. (biomedcentral.com)
Tissue engineering strategies adopting seeding and differentiation of individual chondrocytes on porous 3D scaffolds of clinically relevant size remains a considerable challenge. (desktopmetal.com)
A well documented method to produce small samples of differentiated cartilage tissue in vitro is via micro-mass (pellet) culture, whereby, high concentrations of chondrocytes coalesce to form. (desktopmetal.com)
The first tissue-engineered, ear-shaped appendages made from bovine chondrocytes and biocompatible scaffolds by the Vacanti group were prone to deformation when xenografted onto immune-compromised mice, highlighting the lack of long-term stability [ 6 ]. (biomedcentral.com)
Bovine chondrocytes proliferated and produced a type II collagen and a sulfated glycosaminoglycan-rich extracellular matrix on all scaffolds. (ox.ac.uk)
Optimal amount of basic fibroblast growth factor in gelatin sponges incorporating β-tricalcium phosphate with chondrocytes," Tissue Engineering Part A, vol. 21, pp. 627-636, 2015. (waset.org)

Scientific advances in biomaterials, stem cells, growth and differentiation factors, and biomimetic environments have created unique opportunities to fabricate or improve existing tissues in the laboratory from combinations of engineered extracellular matrices ("scaffolds"), cells, and biologically active molecules. (wikipedia.org)
The three key elements of regenerative medicine and tissue engineering are morphogenetic signals, stem cells, and scaffolds of extracellular matrix. (ucdavis.edu)
Tissue exhibits unique mechanical, biochemical and structural properties, with discrete and continuous changes in cellular and extracellular composition that defines intricate channels, chambers and interfaces. (edu.au)
It is a complex and dynamic process to form a continuous construct via the cells' adhesion, proliferation, differentiation, and extracellular matrix (ECM) deposition on a scaffold. (dovepress.com)
The structural biomimetics are provided by the collagen fibers, which mimic the extracellular matrix of the osteochondral human tissue, and by the low crystallinity of the mineral phase. (materialstoday.com)
Introduction The cells in a tissue are held together by colloidal extracellular matrix (ECM), which is gel like in consistency. (docksci.com)

Three-dimensional biodegradable porous scaffolds play an important role in tissue engineering. (elsevierpure.com)
We created double-layered porous scaffolds composed of synthetic biodegradable polymers by combining freeze-drying and porogen leaching techniques to create tissue- engineered articular cartilage. (elsevierpure.com)
The BioBrace implant is a biocomposite soft tissue scaffold that reinforces tendon and ligament procedures by providing an optimum balance of strength for reinforcement of tissue and porous biologic scaffold for regeneration of tissue. (orthoworld.com)
BioBrace's highly-porous collagen matrix is embedded with bioresorbable polymer microfilaments that provide a strong, open 3D biologic scaffold. (orthoworld.com)
The product is more than 80% porous with interconnected pores and a scaffold structure, meaning that patient cells can quickly integrate and create new tissue within the implant. (orthoworld.com)
Moreover, our findings showed that porous composite scaffolds could be engineered with initial properties that reproduce the anisotropy, viscoelasticity and tension-compression nonlinearity of native articular cartilage. (wustl.edu)
Porous structure, biocompatibility and biodegradability, large surface area, and drug-loading ability are some remarkable properties of zeolite structure, making it a great possible option for bone tissue engineering. (afpm.org.my)
These scaffolds should be biocompatible, biodegradable and porous and support the normal proliferation and functioning of the cells seeded on to it (Gnanaprakasam Thankam et al. (docksci.com)
The aim of this experimental study is to predict the long-term mechanical behavior of a porous scaffold implanted in a cartilage defect for tissue engineering purpose. (upv.es)

Our work aims to develop optimal tissue engineered constructs for the repair and regeneration of different types of musculoskeletal tissues, including bone, cartilage and tendon. (edu.au)
Current tissue-engineered auricular constructs implanted into immune-competent animal models have been observed to undergo inflammation, fibrosis, foreign body reaction, calcification and degradation. (biomedcentral.com)
While many types of matrices have been successfully applied in the clinic, fundamental questions remain about how to combine and manipulate cells with scaffolding to generate functional tissue constructs that avoid immunogenicity. (genengnews.com)
After femoral and tibial osteotomy and autologous implantation of tissue-engineered constructs in rabbit knee joints, implant fixation and joint articulation were evaluated. (desktopmetal.com)
Here we present a microscale 3D weaving technique to generate anisotropic 3D woven structures as the basis for novel composite scaffolds that are consolidated with a chondrocyte-hydrogel mixture into cartilage tissue constructs. (wustl.edu)
In vitro culture increases mechanical stability of human tissue engineered cartilage constructs by prevention of microscale scaffold buckling. (ndriresource.org)

Effects of an articular cartilage lubrication with a viscosupplement in vitro and in vivo following osteochondral fractures in horses. (ucsd.edu)
Trilaminar composite scaffolds were then compared to homogeneous aligned or randomly oriented fiber scaffolds to assess in vitro cartilage formation. (ox.ac.uk)
Our results demonstrate that trilaminar composite scaffolds mimic key organizational characteristics of native cartilage, support in vitro cartilage formation, and have superior mechanical properties to homogenous scaffolds. (ox.ac.uk)
Accordingly, tissue engineering could overcome these limitations by producing in vitro cartilage substitutes. (bvsalud.org)
To enhance the osteogenic potential of stem cell sheet, we fabricated bone morphogenetic protein 2 ( BMP-2 ) gene-engineered cell sheet using a complex of polyethylenimine-alginate (PEI-al) nanocomposites plus human BMP-2 complementary(c)DNA plasmid, and studied its osteogenesis in vitro and in vivo. (dovepress.com)
Effects of scaffold pore architecture on rabbit chondrocyte differentiation and mechanical properties were evaluated following in vitro culture and subcutaneous implantation in nude mice. (desktopmetal.com)
Apatite formation on the scaffold surface was determined with Simulation body fluid (SBF), and a new bone-like apatite layer shaping on all samples confirmed the in vitro bioactivity of ZSM-5-GEN scaffolds. (afpm.org.my)
The in vitro construction of osteo-articular large implants combining biomaterials and cells is of great interest since these tissues have limited regeneration capability. (techno-press.com)

The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues. (wikipedia.org)
In recent years, the lab has identified stem and progenitor cells in articular cartilage, muscle and synovial tissues. (ucdavis.edu)
A number of recent studies have developed tissue engineering approaches for promoting cartilage repair using undifferentiated progenitor cells seeded on biomaterial scaffolds, but little is known about how oxygen might influence these engineered tissues. (duke.edu)

In terms of biomaterials used for CTE, three-dimensional (3D) self-assembling peptide scaffolds (SAPS) are very attractive for their unique properties, such as biocompatibility, optional possibility of rationally design cell-signaling capacity, biodegradability and modulation of its biomechanical properties. (intechopen.com)
Our tissue engineering research focuses on repairing diseased or damaged tissues, incorporating the areas of biomaterials, stem cells, computer modelling and molecular biology. (edu.au)
Tissue engineering and regenerative medicine can provide a novel treatment regime based on the use of synthetic biomaterials, which may be constructed into three-dimensional implants and combined with biologics (such as cells and/or bioactive molecules). (edu.au)
Current research in tissue engineering is geared towards elucidating the appropriate compositional elements (biomaterials, biomolecules and cell sources) as well as methods of assembly. (biomedcentral.com)
Collagen hydrogel as an immunomodulatory scaffold in cartilage tissue engineering," Journal of Biomedical Materials Research Part B: Applied Biomaterials, vol. 102, pp. 337-344, 2014. (waset.org)
Tissue Engineering Using Ceramics and Polymers, Third Edition is a valuable reference tool for both academic researchers and scientists involved in biomaterials or tissue engineering, including the areas of bone and soft-tissue reconstruction, repair and organ regeneration. (elsevier.com)
Technology and research in the field of tissue engineering has drastically increased within the last few years to the extent that almost every tissue and organ of the human body could potentially be regenerated with the aid of biomaterials. (elsevier.com)
The current availability of diverse biomaterials (synthetic and natural) allows a variety of combinations with cells to mimic corresponding human tissues. (rheolution-store.com)
To date, numerous stem cells and biomaterials have been explored for a variety of tissue and organ regeneration. (routledge.com)
Mikos' research focuses on the synthesis, processing, and evaluation of new biomaterials for use as scaffolds for tissue engineering, as carriers for controlled drug delivery, as non-viral vectors for gene therapy, and as platforms for disease modeling. (rice.edu)
Mikos is a founding editor and editor-in-chief of the journals Tissue Engineering Part A, Tissue Engineering Part B: Reviews, and Tissue Engineering Part C: Methods and a member of the editorial boards of the journals Advanced Drug Delivery Reviews, Cell Transplantation, Journal of Biomaterials Science Polymer Edition, Journal of Biomedical Materials Research (Part A and B), and Journal of Controlled Release. (rice.edu)
He is Past-President of the Tissue Engineering and Regenerative Medicine International Society-Americas and the Society For Biomaterials. (rice.edu)

Thus, IGF has great potential to promote the regeneration of articular cartilage after injur y[ 5 ]. (biomedcentral.com)

The insulin-like growth factor (IGF) signaling pathway has been implicated in articular cartilage repair. (biomedcentral.com)

The finding that the use of our tissue engineered composite (the "protein-platelet scaffold) could stimulate healing of a central defect led us to combine the use of this scaffold with a suture repair (a "bioenhanced repair") to treat a complete ACL tear. (childrenshospital.org)
Compressive testing at physiological strain levels further demonstrated that the mechanical properties of trilaminar composite scaffolds approached those of native cartilage. (ox.ac.uk)
Composite scaffolds show mechanical properties of the same order of magnitude as values for native articular cartilage, as measured by compressive, tensile and shear testing. (wustl.edu)
A group of researchers from the AO Research Institute Davos led by Dr. Matteo D'Este developed a bioink containing both collagen (Col) type 1 and tyramine derivative hyaluronan (THA) as a promising composite biomaterial for connective tissue engineering. (rheolution-store.com)
After an incubation of 30 min at 37 °C, the 3D-scaffold was printed thanks to the shear-thinning properties of the composite bioink. (rheolution-store.com)

Beyond augmenting ACL and rotator cuff repairs, Biorez is considering targeted indications such as the Achilles tendon, the ulnar collateral ligament of the elbow, extra articular ligaments of the knee, and others. (orthoworld.com)

Traditional BTE involves three-dimensional biodegradable scaffolds, stem cells, and osteoinductive growth factors. (dovepress.com)

Building upon this work we are exploring the differentiation of embryonic and adult stem cells for articular cartilage and bone repair. (ucdavis.edu)
We use auricular cartilage as an exemplar to illustrate how the use of tissue-specific adult stem cells, assembly through additive manufacturing and improved understanding of postnatal tissue maturation will allow us to more accurately replicate native tissue anisotropy. (biomedcentral.com)
However, the triad components - stem cells, morphogenic agents, and scaffolds - need further improvement before clinical application. (bvsalud.org)
Approaches to scaffolding technology that were discussed at the meeting ranged in complexity from tissue repair products consisting of bovine type 1 collagen knee implants to the highly complex such as the use of whole acellular lung matrix (ACM) to support the development of engineered lung tissue from embryonic stem cells. (genengnews.com)
Tissue engineers working at the laboratory for stem cells and tissue engineering at Columbia University's Fu Foundation School of Engineering are applying a biomimetic approach to developing tissue replacements. (genengnews.com)
The availability of personalized bone grafts engineered from the patient's own stem cells, he said "would revolutionize the way we currently treat defects. (genengnews.com)
For their bone reconstruction model, the scientists generated anatomically shaped scaffolds in the exact shape of the human temperomandibular joint (TMJ) bone from decellularized trabecular bone using digitized clinical images, seeded with human mesenchymal stem cells, and cultured with interstitial flow of culture medium. (genengnews.com)
Recently, stem cell sheet has been an emerging strategy in bone tissue engineering. (dovepress.com)
Our results suggested that PEI-al nanocomposites efficiently deliver the BMP-2 gene to bone marrow mesenchymal stem cells and that BMP-2 gene-engineered cell sheet is an effective way for promoting bone regeneration. (dovepress.com)
Epigenetic approaches using the histone deacetylase 2 and 3 inhibitor-MI192 have been reported to accelerate stem cells to form mineralised tissues. (desktopmetal.com)
Micro- and nanotechnology hold great potential to fabricate biomimetic spatiotemporally controlled scaffolds as well as control stem cell behavior and fate by micro- and nanoscale cues. (routledge.com)
Mesenchymal Stem Cells in the Musculoskeletal System: From Animal Models to Human Tissue Regeneration? (educell.si)
Tissue engineering is an exciting strategy that employs stem cells, scaffolds and growth factors or mediators either in isolation or in combination. (openorthopaedicsjournal.com)
Many tissue engineering advances are being made in orthopaedics and this is partly due to the familiarity with bone marrow derived mesenchymal stem cells. (openorthopaedicsjournal.com)
In this review we will cover mesenchymal stem cells, scaffolds, bioreactors and growth factors applicable to orthopaedics and musculoskeletal sciences. (openorthopaedicsjournal.com)
This is followed by specific discussions on Dupuytren's disease where there is early evidence of stem cell etiology, and bone and cartilage tissue engineering. (openorthopaedicsjournal.com)
Over the last decade, there has been considerable interest in the use of mesenchymal stem cells and tissue engineering principles in orthopaedics and musculoskeletal sciences. (openorthopaedicsjournal.com)
Cells with mesenchymal stem cell characteristics have been isolated from many different adult tissues including bone marrow, periosteum, skin, adipose tissue, skeletal muscle, synovial tissue, the infrapatellar fat pad, and more recently, cartilage [ 8 - 15 ], and we provide a systematic review of the literature outlining the sources of mesenchymal stem cell used in musculoskeletal applications. (openorthopaedicsjournal.com)

In the short term, Mr. Rocco said the company is focusing on clinical indications with the most need, such as augmenting tissue grafts used in ACL surgeries, augmenting large rotator cuff tears and other situations where there is a high risk of failure with existing techniques. (orthoworld.com)
The most studied devices at the laboratory are cardiac valves, small-diameter vascular grafts and scaffolds for skin wound healing. (cnr.it)

1] S.-M. Lien, L.-Y. Ko, and T.-J. Huang, "Effect of pore size on ECM secretion and cell growth in gelatin scaffold for articular cartilage tissue engineering," Acta Biomaterialia, vol. 5, pp. 670-679, 2009. (waset.org)

Articular cartilage lesions, which can progress to osteoarthritis, are a particular challenge for regenerative medicine strategies, as cartilage function stems from its complex depth-dependent microstructural organization, mechanical properties, and biochemical composition. (ox.ac.uk)
We fabricated trilaminar scaffolds by sequential electrospinning and varying fiber size and orientation in a continuous construct, to create scaffolds that mimicked the structural organization and mechanical properties of cartilage's collagen fibrillar network. (ox.ac.uk)
An artificial tissue should have some features such as biocompatibility, biodegradation and, enough mechanical properties like the original tissue. (waset.org)
6] A. Karimi and M. Navidbakhsh, "Mechanical properties of PVA material for tissue engineering applications," Materials Technology, vol. 29, pp. 90-100, 2014. (waset.org)
7 However, the scaffolds still face the problem of biocompatibility, mechanical properties, and bioresorbability, which are not adaptable to the properties of natural bone. (dovepress.com)
The rapid restoration of tissue biomechanical function remains an important challenge, emphasizing the need to replicate structural and mechanical properties using novel scaffold designs. (wustl.edu)
Overall, the mechanical properties of the final printed scaffold weren't measured. (rheolution-store.com)
We hypothesize that CNT-based material in tissue engineering can provide an improved molecular sized substrate for stimulation of cellular growth, and structural reinforcement of the scaffold mechanical properties. (asme.org)

Dr. Freytes explained that bone reconstructions, such as craniofacial reconstruction, often involve "autologous tissue grafting, a method limited by harvesting difficulties, donor site morbidity, and the clinicians' ability to contour delicate 3-D shapes. (genengnews.com)
The gold standard of clinical therapies relies on replacing the defect with autologous tissue from a non load-bearing site, yet engineered tissue often fails due to a lack of mechanical anchoring, donor site morbidity and tissue availability. (colorado.edu)

Biocompatibility studies showed positive observation in biosafety test using direct contact cytotoxicity assay in addition to active cellular growth on the hydrogel scaffold based on fluorescence observation. (afpm.org.my)
In the current study, novel polyethylene glycol interpenetrated cross-linked hydrogel scaffold based on a co-polysaccharide (PIAC) synthesized from two marine heteropolysaccharides, alginate and chitosan, was designed. (docksci.com)

An ex vivo study showed that IGF-1 in fetal bovine serum was responsible for maintaining articular cartilage proteoglycan synthesis. (biomedcentral.com)

Osteochondral tissue engineering using multilayer hydrogel scaffolds to recapitulate the native layered structure serves as a promising strategy to repair and regenerate osteochondral defects due to joint trauma, injuries, and long-term diseases. (colorado.edu)
This thesis demonstrated that cell-mediated degradation and loading within multilayer hydrogels can create an osteogenic scaffold to promote tissue regeneration for osteochondral tissue engineering applications. (colorado.edu)

Anisotropic fibrous scaffolds for articular cartilage regeneration. (ox.ac.uk)

Cartilage tissue engineering (CTE) aims to produce cartilage-like tissues that recreate the complex mechanical, biophysical and biological properties found in vivo. (intechopen.com)
Our initial in vivo experiments focused on a central defect model in which a cut is created within the ACL midsubstance and then left to heal on its own or after treatment with a protein scaffold loaded with platelets. (childrenshospital.org)
Moreover, one should consider the application of mechanical stimuli and surface properties to produce an articular cartilage with satisfactory characteristics for in vivo application. (bvsalud.org)
Such scaffolds uniquely combine the potential for load-bearing immediately after implantation in vivo with biological support for cell-based tissue regeneration without requiring cultivation invitro. (wustl.edu)
Such a scaffold can be made from synthetic and natural molecules or a combination of both, which can be effectively used for the ex vivo engineering of various organ parts. (docksci.com)
A model is proposed to assess mechanical behaviour of tissue engineering scaffolds and predict their performance in vivo during tissue regeneration. (upv.es)

We aim to gain a better understanding of the relationship between T cell functions and their physical microenvironment by 3D printing hydrogels as a model for the soft tissues and organs in our bodies. (edu.au)
Gelatine methacryloyl (GelMA) hydrogels provide a favourable microenvironment to facilitate cell delivery and support tissue formation. (desktopmetal.com)
PEG-penetrated chitosan-alginate co-polysaccharide-based partially and fully cross-linked hydrogels as ECM mimic for tissue engineering applications. (docksci.com)

Adult articular cartilage presents poor intrinsic capacity for regeneration, and after injury, cellular or biomaterial-based therapeutic platforms are required to assist repair promotion. (intechopen.com)
Tissue engineering seeks to repair or regenerate tissues through combinations of implanted cells, biomaterial scaffolds and biologically active molecules. (wustl.edu)
Such an interaction between cells and materials represents the focus of both current bone tissue engineering strategies and biomaterial science. (materialstoday.com)
Cucchiarini, M., Madry, H. Biomaterial-guided delivery of gene vectors for targeted articular cartilage repair. (uniklinikum-saarland.de)

Articular cartilage repair is a critical issue in osteoarthritis (OA) treatment. (biomedcentral.com)
Osteoarthritis (OA) is characterized by the progressive destruction of articular cartilage, which seriously restricts sports ability and impacts quality of life. (biomedcentral.com)
Wnt7a Inhibits IL-1β Induced Catabolic Gene Expression and Prevents Articular Cartilage Damage in Experimental Osteoarthritis. (ndriresource.org)

Budday, S.: Time-dependent hyper-viscoelastic parameter identification of human articular cartilage and substitute materials. (uni-rostock.de)

The aim of this study is to confirm the usefulness of sonication treatment for preparation of biological scaffolds. (afpm.org.my)

Currently the main activities concern the design and fabrication of bioactive materials for tissue regeneration and for local administration of drugs and bioactive agents in cardiovascular, osteo-articular and wound healing field. (cnr.it)

This 3D bone biomimetic scaffold is composed of type I collagen fibers, biomineralized with HA during fiber self-assembling. (materialstoday.com)

Recent advances in regenerative medicine place us in a unique position to improve the quality of engineered tissue. (biomedcentral.com)
Due to recent advances in regenerative medicine and additive manufacturing we are entering into an age where we have the potential to replace 'like with like', by improving the quality of engineered tissue with respect to biochemical composition and functionality, as well as microstructural organization and overall shape. (biomedcentral.com)
He is organizer of the continuing education course Advances in Tissue Engineering offered annually at Rice University since 1993. (rice.edu)

14] X. Li, J. Xie, X. Yuan, and Y. Xia, "Coating electrospun poly (ε-caprolactone) fibers with gelatin and calcium phosphate and their use as biomimetic scaffolds for bone tissue engineering," Langmuir, vol. 24, pp. 14145-14150, 2008. (waset.org)
2 , 3 Bone tissue engineering (BTE) attempts to solve the referred problems mentioned. (dovepress.com)
Human bone marrow stromal cells (hBMSCs) have been extensively utilised for bone tissue engineering applications. (desktopmetal.com)
This is mainly because these substitutes have been unable to reproduce all of the required cartilage and bone tissue characteristics. (materialstoday.com)
Bone tissue is composed of hydroxyapatite (HA) nanocrystals, grown on collagen fibers, morphologically and structurally organized up to the molecular scale. (materialstoday.com)
Seitz, H.: 3D Printing of Piezoelectric and Bioactive Barium Titanate-Bioactive Glass Scaffolds for Bone Tissue Engineering. (uni-rostock.de)

Combining biomimetic regenerative medicine strategies will allow us to improve tissue-engineered auricular cartilage with respect to biochemical composition and functionality, as well as microstructural organization and overall shape. (biomedcentral.com)
We propose that these scaffolds offer promise in regenerative medicine strategies to repair articular cartilage lesions. (ox.ac.uk)

The device is anatomically shaped like a human meniscus and is intended, when sutured to the meniscus rim, to act as a tissue scaffold and fill the void left after meniscus loss. (genengnews.com)

Furthermore, all scaffolds promoted significant upregulation of aggrecan and type II collagen gene expression while downregulating that of type I collagen. (ox.ac.uk)
John Dichiara, svp of clinical and regulatory affairs at ReGen Biologics, discussed the clinical performance of ReGen's collagen surgical mesh scaffold used for the reinforcement and repair of medial meniscus injuries. (genengnews.com)
The BioBrace device includes a highly absorbent microporous collagen scaffold reinforced with resorbable polymer microfilaments. (orthoworld.com)
These scaffolds can be designed with different compositional gradients varying the HA-collagen ratio [3]. (materialstoday.com)
This month's cover image shows human osteoblast-like cells completely embedded within the nanostructure of a bio-inspired collagen-HA scaffold. (materialstoday.com)
In their study entitled " Tissue mimetic hyaluronan bioink containing collagen fibers with controlled orientation modulating cell migration and alignment " published in the journal Materials Today Bio , the group aimed to print a homogeneous 3D hydrogel with a controlled Col fibril orientation at the microscopic level and evaluated the impact of this anisotropic architecture on embedded human bone marrow-derived mesenchymal stromal cell (hMSC). (rheolution-store.com)
Using concentrations and ratios previously defined optimal for cartilaginous tissue engineering, both biopolymers and cells were mixed, before the induction of simultaneously two mild crosslinking pathways: (i) the fibrillogenesis and physical gelation of collagen triggered with the pH increase up to 7, and (ii) the enzymatic chemical crosslinking of THA at 37 °C (optimal functioning temperature for the enzyme). (rheolution-store.com)

With about one million connective tissue repairs or reconstructions per year in the United States alone, the market is ripe for innovation. (orthoworld.com)
There's a lot of interest in how to improve the strength and durability of connective tissue repairs as well as accelerate the healing process," said Kevin Rocco, Chief Executive Officer of Biorez. (orthoworld.com)
Articular cartilage is an avascular connective tissue that exhibits little intrinsic capacity for repair. (duke.edu)
In the human body, this connective tissue displays parallel Col fibrils near the surface, and fibrils orthogonal to these, in the depths. (rheolution-store.com)

however, the success of homogeneous scaffolds is limited by their inability to mimic the cartilage's zone-specific organization and properties. (ox.ac.uk)
Research on scaffolds that mimic the three-dimensional organization of physiological tissues is of fundamental importance for treatments in regenerative medicine that aim to reconstruct lost or damaged tissue. (materialstoday.com)
The present hydrogel can form an ECM mimic and can form a potent candidate for various tissue engineering applications. (docksci.com)

We then developed and validated a histologic scoring system to grade the ligament healing response(1) and then tested the hypothesis that placement of a substitute protein scaffold could stimulate functional healing of the ACL. (childrenshospital.org)
The ends of the torn ACL then grow into the protein scaffold and the ligament reunites. (childrenshospital.org)
Typically, ligament and tendon repairs rely on surgical intervention wherein injured tissues are replaced with soft tissue allografts or autografts and/or reinforced with resorbable or permanent devices with the hope that the surgical repair will heal over time. (orthoworld.com)

A recent publication titled "Co-electrospun PCL/Zein membranes as scaffolds for articular cartilage engineering" by the ETH team was featured in MDPI Bioengineering. (biotrib.eu)
She is also director of the Bioengineering Laboratory for Nanomedicine and Tissue Engineering at the university. (routledge.com)
Antonios G. Mikos is the Louis Calder Professor of Bioengineering and Chemical and Biomolecular Engineering at Rice University. (rice.edu)

At the same time, the loading of cells onto scaffolds is a critical step in developing self-sustained scaffolds for in situ implantation, and a proper equilibrium must be reached. (materialstoday.com)

While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e. bone, cartilage, blood vessels, bladder, skin, muscle etc. (wikipedia.org)
The field of tissue engineering is constantly evolving as it aims to develop bioengineered and functional tissues and organs for repair or replacement. (mdpi.com)
The UC Davis Center for Tissue Regeneration and Repair focuses on regeneration for the musculoskeletal system to restore function to tissue diseased or damaged from cancer, trauma, and arthritis. (ucdavis.edu)
For the complete tear, we developed a model in the porcine knee (Fig 2) and we found that the use of a protein-platelet scaffold could stimulate functional healing following a complete ACL tear(8) (Fig 3), while use of platelets alone(9) or the protein scaffold alone(10) did not improve repair healing. (childrenshospital.org)
Final considerations: Current treatments for articular cartilage repair have major limitations. (bvsalud.org)
Biological scaffolding, either alone or in combination with cells for tissue regeneration, has the potential to revolutionize the repair of injured tissues and to replace tissues lost through disease and injury. (genengnews.com)
In addition, repair or replacement cells or tissues should not provoke immune reactions in the host. (genengnews.com)
In comparison with previous works, this study offers a new and efficient procedure for the fabrication of cartilage like tissue and further cartilage repair. (waset.org)
The BioBrace is designed with tissue matching stiffness and a more open scaffold for the tissue to heal into the repair more quickly. (orthoworld.com)
Orth, P., *Eldracher, M., Cucchiarini, M., Madry, H. Small diameter subchondral drilling improves DNA and proteoglycan contents of the cartilaginous repair tissue in a large animal model of a full-thickness chondral defect Journal of Clinical Medicine , 2020, 18;9(6):E1903. (uniklinikum-saarland.de)
Many musculoskeletal tissues are frequently damaged or lost in injury and disease, and show a limited capacity for repair. (openorthopaedicsjournal.com)

These are usually relatively large, dense pieces of tissue requiring a long time for the body to biologically re-integrate into the repaired site. (orthoworld.com)
Ali Khademhosseini is associate professor at the Harvard-MIT Division of Health Sciences and Technology, Brigham and Women's Hospital, and Harvard Medical School as well as associate faculty at the Wyss Institute for Biologically Inspired Engineering, Harvard University, USA. (routledge.com)

Tissue engineering is a biomedical engineering discipline that uses a combination of cells, engineering, materials methods, and suitable biochemical and physicochemical factors to restore, maintain, improve, or replace different types of biological tissues. (wikipedia.org)
Tissue engineering often involves the use of cells placed on tissue scaffolds in the formation of new viable tissue for a medical purpose but is not limited to applications involving cells and tissue scaffolds. (wikipedia.org)
In addition, Langer and Vacanti also state that there are three main types of tissue engineering: cells, tissue-inducing substances, and a cells + matrix approach (often referred to as a scaffold). (wikipedia.org)
He proposed the joining of the terms tissue (in reference to the fundamental relationship between cells and organs) and engineering (in reference to the field of modification of said tissues). (wikipedia.org)
The fundamental concept combines various tissue engineering elements, most often a scaffold as a supporting matrix in combination with living cells and/or bioactive molecules, to form a tissue engineering construct that repairs or regenerates the diseased or damaged tissue or organ. (edu.au)
Objective: To review the literature regarding sources of cells, scaffolds, and morphogenic agents currently used to produce articular cartilage. (bvsalud.org)
In this context, tissue engineering is an emerging and multidisciplinary field, which three main components are responsive cells, scaffolds, and morphogenic agents. (bvsalud.org)
Assays on the cell culture showed that the genetically engineered cells released the BMP-2 for at least 14 days. (dovepress.com)
It's cell-friendly, meaning that a patient's cells can grow directly into the implant to rebuild new functional tissue. (orthoworld.com)
This study aimed to investigate a GelMA hydrogel reinforced with a 3D printed scaffold to support MI192-induced human bone marrow stromal cells (hBMSCs) for bone formation. (desktopmetal.com)
In the case of osteochondral regeneration, the scaffolds must reproduce the complex physico-chemical features of the articular cartilage and of the underlying subchondral bone, from the macro- to nano-scale and provide the right environment for cellular activity, favouring cell colonization, and assuring the appropriate communication between cells and the material. (materialstoday.com)
1997). So the need of ECM substitutes that hold the cells until the rejuvenation of host tissue, post-disease stages, is highly demanding. (docksci.com)
An ECM substitute should act as a scaffold to carry the cells until a functional tissue is reestablished (Finosh and Jayabalan 2012). (docksci.com)
The the goal of this thesis was to create a multilayer poly(ethylene glycol) (PEG) hydrogel platform, with a bony layer that facilitates cellular mediated degradation to allow for tissue elaboration and osteogenesis of encapsulated cells under physiologically relevant loading regimes. (colorado.edu)
These findings suggest oxygen tension may play an important role in regulating the proliferation and metabolism of hADAS cells as they undergo chondrogenesis, and the exogenous control of oxygen tension may provide a means of increasing the overall accumulation of matrix macromolecules in tissue-engineered cartilage. (duke.edu)
Within projects concerning innovative biocompatible materials and tissue engineering, the laboratory has gained experience in the culture of cell lines and in the isolation of human primary cells. (cnr.it)

The current paradigm in sports medicine is to reapproximate soft tissues and wait for the tissue to heal. (orthoworld.com)

Thereby, reproducing anisotropic tissues - non-uniform tissues that have properties and/or structure oriented in a specific direction, like e.g. bones, cartilage or intervertebral discs - remains today a challenge. (rheolution-store.com)
Finally, the architecture of the printed scaffold was compared with the native knee articular cartilage, set in this study as an anisotropic 3D-bioprinting challenge. (rheolution-store.com)

Background: Preliminary studies investigated advanced scaffold design and tissue engineering approaches towards restoring congruent articulating surfaces in small joints. (desktopmetal.com)
In addition to cartilage tissue engineering, the CRL applies similar approaches and technologies towards cardiovascular and cancer applications. (edu.au)

Through co-electrospinning, the team successfully reduced protein adsorption by half compared to scaffolds made solely of bovine serum albumin and equine synovial fluid. (biotrib.eu)

Moreover, magnetic HA can be nucleated into the material, inducing a super-paramagnetic effect which improves fixation in complex articular sites [4]. (materialstoday.com)
Successful Fixation of Traumatic Articular Cartilage-Only Fragments in the Juvenile and Adolescent Knee: A Case Series. (mayo.edu)

Among the major challenges now facing tissue engineering is the need for more complex functionality, biomechanical stability, and vascularization in laboratory-grown tissues destined for transplantation. (wikipedia.org)

Due to their large surface area and ability to interact with proteins and peptides, graphene oxides offer valuable physiochemical and biological features for biomedical applications and have been successfully employed for optimizing scaffold architectures for a wide range of organs, from the skin to cardiac tissue. (mdpi.com)
The worldwide shortage of donor organs and drawbacks of surgical methods have created significant challenges in repairing and replacing diseased or damaged tissues and organs. (edu.au)
The ECM component of the tissues forms an insoluble scaffold, which determines the shape and dimension of the organs (Cox and Erler 2011). (docksci.com)

2013). Incorporation of physiochemical, biological and mechanical cues along with these scaffolds improves the quality and durability of the ECM substitutes. (docksci.com)

Anatomy50

Organisms8

Diseases5

Chemicals and Drugs66

Analytical, Diagnostic and Therapeutic Techniques and Equipment62

Phenomena and Processes39

Disciplines and Occupations15

Technology, Industry, Agriculture38

Humanities1

Information Science4

Health Care1

Intra-articular5

Chondrocytes8

Extracellular6

Porous9

Constructs6

Vitro8

Progenitor cells3

Biomaterials12

Regeneration of articular cartilage1

Implicated in articular cartilage1

Composite5

Extra-articular1

Biodegradable1

Stem18

Grafts2

Acta Biomaterialia1

Mechanical properties8

Autologous2

Hydrogel scaffold based2

Maintaining articular cartilage1

Osteochondral tissue2

Fibrous scaffolds1

VIVO6

Hydrogels3

Biomaterial4

Osteoarthritis3

Human articular1

Biological Scaffolds1

Osteo-articular1

Biomimetic scaffold1

Advances3

Bone tissue6

Regenerative medicine strategies2

Anatomically shaped1

Collagen7

Connective tissue4

Mimic3

Ligament3

Bioengineering3

Situ1

Repair11

Biologically2

Cells16

Soft tissues1

Anisotropic2

Approaches2

Bovine1

Fixation2

Transplantation1

Organs3

Improves1