Arrest of cells indicates cell cycle. Medical search. Frequent questions

Tan IIA inhibited proliferation of mouse keratinocytes in a dose- and time-dependent manner and induced apoptosis, resulting in S phase arrest accompanied by down-regulation of pCdk2 and cyclin A protein expression. (hindawi.com)
Thus, unlike other tumor suppressors, RPL5/RPL11 play an essential role in normal cell proliferation, a function cells have evolved to rely on in lieu of a cell cycle checkpoint. (nih.gov)
Functional analysis revealed that knockdown of PTGR1 significantly inhibited proliferation and colony formation by PC cells. (go.jp)
The inhibition of cell proliferation was related to arrest of the cell cycle in the G0/G1 phase and increased apoptosis in response to PTGR1 knockdown as indicated by flow cytometry. (go.jp)
Taken together, our results suggested that decreased LAPTM5 inhibited proliferation and viability, as well as induced G0/G1 cell cycle arrest possibly via deactivation of ERK1/2 and p38 in BCa cells. (spandidos-publications.com)
Furthermore, previous studies suggested that knockdown of LAPTM4B , another important subtype of the LAPTM family inhibited proliferation of hepatocellular carcinoma ( 11 ), prostate ( 12 ) and breast cancer cells ( 13 ). (spandidos-publications.com)
The expression of cyclin-dependent kinase inhibitors was profoundly affected with early activation and then repression of p21 cip1/waf1 and persistent activation of both p27 kip1 and p57 kip2 , whereas genes involved in cell survival and proliferation were suppressed. (aacrjournals.org)
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)-test was used to observe the proliferation of human breast cancer MCF-7 and MDA-MB-231 cells induced on VALD-3. (researchsquare.com)
Our study demonstrates that survivin and APE1/Ref-1 are significantly higher in human prostate cancer specimens compared to noncancerous controls and that APE1/Ref-1 redox-specific inhibition with small molecule inhibitor, APX3330 and a second-generation inhibitor, APX2009, decreases prostate cancer cell proliferation and induces cell cycle arrest. (oncotarget.com)
The current study demonstrated that PD markedly inhibited PAC cell proliferation and triggered their apoptosis. (biomedcentral.com)
Dichloroacetate (20 mM) did not reduce growth of non-cancerous cells but caused significant decrease in cancer cell proliferation ( P =0.009), which was associated with apoptosis and G 2 phase cell-cycle arrest. (dcaguide.org)
We appraised the antitumour effects of aspirin on cell proliferation and tumour growth , cell cycle distribution, apoptosis , signalling pathways, angiogenesis-related proteins , and phosphorylated receptor tyrosine kinases (p-RTKs) and identify miRNAs associated with its antitumour effects. (bvsalud.org)
Aspirin inhibited cell proliferation in pancreatic cancer cell lines and induced G0/ G1 cell cycle arrest by decreasing the expression of cyclin D1 . (bvsalud.org)
Aspirin inhibited the proliferation of pancreatic cancer cells and induced cell cycle arrest . (bvsalud.org)
Apoptotic death is preceded by proliferation blockage, suggesting that these transcripts are involved in cell cycle regulation. (uandes.cl)
Our results suggest that ASncmtRNA targeting blocks tumor cell proliferation through reduction of essential cell cycle proteins, mediated by mitochondrial and nuclear miRNAs. (uandes.cl)
Open up in another window Shape 2 Leucine deprivation inhibits breasts tumor cell proliferation(A) MDA-MB-231 or CORIN MCF-7 cells had been seeded in 12-well plates and incubated in charge (+leu) or leucine-deficient (?leu) moderate. (racetab.org)
This study investigates the role of impaired proliferation, altered cell cycle arrest, and defective autophagy flux of corneal fibroblasts in granular corneal dystrophy type 2 (GCD2) pathogenesis. (elsevierpure.com)
These data provide new insight concerning the role of autophagy in cell cycle arrest and cellular proliferation, uncovering a number of novel therapeutic possibilities for GCD2 treatment. (elsevierpure.com)
ASHE suppressed liver cancer cell proliferation by inducing cell cycl e arrest at the G0/G1 phase, as well as apoptosis, as indicated by t he increased number of Annexin V and 7 ‑ AAD ‑ positive cells. (lab-sunchlorella.com)
During the natural developmental process of Drosophila, the developing eye shows a remarkable, tight coordination between cell proliferation and the steps for cell specialisation. (europa.eu)
MAPK pathways refer to various cellular functions, including cell proliferation, differentiation and migration. (springer.com)
In addition, we studied the effect of WSC on the proliferation and cell cycle of alveolar type II cells and vascular endothelial cells. (who.int)

Induced cell cycle arrest is the use of a chemical or genetic manipulation to artificially halt progression through the cell cycle. (wikipedia.org)
The cells can be induced to arrest as they arrive (at different time points) at a certain phase, so that when the arrest is lifted (for instance, rescuing cell cycle progression by introducing another chemical) all the cells resume cell cycle progression at the same time. (wikipedia.org)
Mimosine is a plant amino acid that has been shown to reversibly inhibit progression beyond G1 phase in some human cells, including lymphoblastoid cells. (wikipedia.org)
The use of serum deprivation - partially or completely removing the serum and its nutrients - has been shown to arrest and synchronize cell cycle progression in G0 phase, for example in neonatal mammalian astrocytes and human foreskin fibroblasts. (wikipedia.org)
It further inhibited cell-cycle progression in the G1 phase by four different mechanisms: rapid downregulation of cyclin D1, induction of Chk2 with simultaneous downregulation of Cdc25A, induction of the Cdk-inhibitor p21 Cip/Waf and inhibition of ribonucleotide reductase activity resulting in reduced dCTP and dTTP levels. (nature.com)
Unexpectedly, we found that their depletion in primary human lung fibroblasts failed to induce cell cycle arrest but strongly suppressed cell cycle progression. (nih.gov)
We show that the effects on cell cycle progression stemmed from reduced ribosome content and translational capacity, which suppressed the accumulation of cyclins at the translational level. (nih.gov)
We studied the effect of doxorubicin (Dox) on cell cycle progression and its correlation with DNA damage and cytotoxicity in p53-mutant P388 cells. (aspetjournals.org)
In untreated control P388 cells, p34cdc2 kinase localizes in the nucleus and cytoplasms, particularly in the centrosomes, and p34cdc2 kinase activity is dependent on cell cycle progression, with the enzyme activity increasing steadily from G1/S to G2/M and markedly declining thereafter. (aspetjournals.org)
Cell cycle analysis suggested activation of apoptosis, without arresting cell cycle progression. (nature.com)
A novel UCS cell line (PF338) was established from the malignant pleural effusion of a 59-year-old patient at time of disease progression. (por-journal.com)
Importantly, UCS is the prototype tumor for epithelial-mesenchymal transition (EMT), a reversible biological process that associates with tumor progression and metastasis and in which epithelial cells transform into more invasive mesenchymal cells by losing their epithelial properties [ 10 - 12 ]. (por-journal.com)
This effect is mediated by downregulation of the key cell cycle progression factors cyclin B1, cyclin D1, CDK1, CDK4, and survivin, the latter also constituting an essential inhibitor of apoptosis, underlying additionally the onset of apoptosis. (uandes.cl)
In the present study, we provide evidence that this aberrantly hyperphosphorylated protein is p34(cat2) (cdc2) a key regulator of cell-cycle progression. (uaeu.ac.ae)
These findings provide evidence that, in mature T lymphocytes, cell-cycle progression through the G 2 -M check point requires expression of the Src-family protein tyrosine kinase, Lck. (uaeu.ac.ae)
Flow cytometry indicated a decreased G 1 cell cycle progression and the accumulation of cells in the S and G 2 /M phases in GCD2 cells. (elsevierpure.com)
Flow cytometry indicated a decreased G1 cell cycle progression and the accumulation of cells in the S and G2/M phases in GCD2 cells. (elsevierpure.com)
Therefore, understanding how the division of the cells, i.e. the cell cycle, is coordinated with their responses to the external clues is key to understanding the causes of the formation and progression of tumours as well as the pathology of degenerative disorders, which will ultimately help develop new therapeutic strategies to these diseases. (europa.eu)

It is an artificial activation of naturally occurring cell cycle checkpoints, induced by exogenous stimuli controlled by an experimenter. (wikipedia.org)

Expression of cell cycle inhibitors, P21 and P27, and activation of apoptosis executer enzyme, caspase-3, were also induced by TCEE. (hindawi.com)
This TCEE also demonstrates antitumor properties such as the induction of cell cycle arrest and activation of apoptosis on human colon, lung, melanoma, osteosarcoma, and pancreatic cancer cells [ 16 - 19 ]. (hindawi.com)

While in G1 the cell synthesizes messenger RNA (mRNA) and proteins in preparation for subsequent steps of interphase leading to mitosis. (wikipedia.org)
The expression of pro-apoptotic proteins and anti-apoptotic proteins in MCF-7 and MDA-MB-231 cells was investigated by Western blotting. (researchsquare.com)
In addition, proteome analysis of NBPF1-overexpressing DLD1 cells identified 32 differentially expressed proteins, of which several are implicated in carcinogenesis. (biomedcentral.com)
We demonstrated that NBPF1 exerts different tumor suppressive effects, depending on the cell line analyzed, and provide new clues into the molecular mechanism of the enigmatic NBPF proteins. (biomedcentral.com)
Cell cycle and apoptotic regulatory proteins were determined by western blot analysis. (upm.edu.my)
One of the critical factors for the control of the protein levels during the cell cycle is the Anaphase Promoting Complex/Cyclosome (APC/C). This complex recognises proteins with specific signatures (or degrons) and targets them for degradation via the protein destruction machinery called the proteasome. (europa.eu)
To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)

Our data suggest that HDAC inhibition is effective in a novel UCS cell line by interfering with both viability and differentiation. (por-journal.com)
Induction of p21 expression by genotoxic stress and its role during terminal differentiation of various cell types have been investigated intensively. (bio2009.org)
While p21 is usually activated by p53-dependent mechanisms in response to DNA damage to make sure cell cycle arrest and repair a number of realtors that promote differentiation like phorbol ester 67346-49-0 or okadaic acidity can up-regulate p21 separately of p53 (for an assessment see reference point 16). (bio2009.org)
The inhibitors demonstrated promising effects in vitro with reduced viability, increased differentiation and cell death of neuroblastoma cells. (lu.se)
Subsequent differentiation allows for rear- analysis of a set of mouse B lineage cell lines rep- rangements of the Ig light-chain (IgL) genes that replace the resenting defined stages of B cell development us- surrogate light-chain genes on the surface of the B cell [8]. (lu.se)

M: A mutant screen of budding yeasts with mitotic arrest identified CDC16, CDC23, and CDC27 as key genes that, when mutated, cause arrest in mitosis. (wikipedia.org)
We investigated by real-time quantitative RT-PCR the expression profile of a panel of genes important in cell cycle regulation. (biomedcentral.com)
Modulation of pro and anti-apoptotic genes in cervical cancer cells after exposure to MNBE. (biomedcentral.com)
It controls several genes that play a role in the arrest of the cell cycle, cellular senescence, DNA repair system, and apoptosis. (darkskiesfilm.com)
This then leads to the activation of a number of genes whose products trigger cell-cycle arrest, apoptosis, or DNA repair. (darkskiesfilm.com)
The p53 tumour-suppressor guards the genome in response to genotoxic stress by transcriptional regulation of genes involved in cell-cycle control, DNA replication, repair and apoptosis such as p21, GADD45, bax and mdm2 (Cox and Lane, 1995). (hal.science)
In UCS, TP53 has been identified as the most frequently mutated gene, followed by mutations in the PI3K pathway, KRAS , cell cycle regulators including FBXW7 and chromatin remodeling and histone genes including ARID1A [ 7 - 9 ]. (por-journal.com)
The transcriptional activation from CACNA1F the p21 gene by these inhibitors is normally marketed by chromatin redecorating pursuing acetylation of histones H3 and H4 in the p21 promoter area (32 54 This activation of p21 takes place within a p53-unbiased fashion and for that reason HDAC inhibitors are appealing realtors for cancers therapy being that they are operative in cells with mutated p53 genes a hallmark of several tumors. (bio2009.org)
The majority of cell cycle regulators, including APC/C, as well as the genes regulating cells' responses to external cues, are very similar between Drosophila and mammals, although the genetic circuit in Drosophila is relatively simple and less complex compared to mammals. (europa.eu)
We also assessed the effect of WSC on the expression of genes involved in cell cycle arrest and inflammation. (who.int)

Present findings indicate that T44Bf-mediated antiproliferative effects are associated with a reversible chronic mitotic arrest caused by defects in chromosome alignment, followed by induced programmed cell death. (tmu.edu.tw)
Treatment of neuroblastoma PDX cells with ARRY-520 resulted in mitotic arrest and subsequent cell death. (lu.se)

How does p53 induce cell cycle arrest? (darkskiesfilm.com)
tumor suppressor p53 may induce cell cycle arrest or apoptosis in response to a variety of stress signals such as DNA damage oncogenic stimuli or hypoxia (reviewed in reference 49). (bio2009.org)

Forced expression of NBPF1 in HEK293T cells resulted in a G1 cell cycle arrest that was accompanied by upregulation of the cyclin-dependent kinase inhibitor p21 CIP1/WAF1 in a p53-dependent manner. (biomedcentral.com)
Furthermore, Western blots show that NGF elevated cyclin-dependent kinase inhibitor, p21(WAF1), in TRK1 cells only. (umassmed.edu)

In cell culture, serum is the growth medium in which the cells are grown and contains vital nutrients. (wikipedia.org)
Kaempferol has been shown to inhibit cell growth, induce apoptosis and cell cycle arrest in several tumors, but not in renal cell carcinoma (RCC). (nih.gov)
In the present study, we investigated the effects of kaempferol and the underlying mechanism(s) on the cell growth of RCC cells. (nih.gov)
MTT assay and colony formation assay were used to study cell growth, and flow cytometry was used to study apoptosis and cell cycles in different RCC cells treated with various doses of kaempferol. (nih.gov)
A significant inhibition on cell growth, induction of apoptosis and cell cycle arrest were observed in 786-O and 769-P cells after kaempferol treatment compared with the control group. (nih.gov)
Moreover, the results clearly showed that kaempferol causes a strong inhibition of the activation of the EGFR/p38 signaling pathways, upregulation of p21 expression and downregulation of cyclin B1 expression in human RCC cells, together with activation of PARP cleavages, induction of apoptotic death and inhibition of cell growth. (nih.gov)
Thus, the secretion of gall wasps stimulates plant cell growth and overrules homeostasis of the affected leaf area - this is similar to tumour outgrowth. (nature.com)
In turn, the plant produces GA, which seems to combat the improper growth signals and re-establishes cell-cycle control. (nature.com)
In the present study, the TCEE treatment induced cell cycle arrest and suppressed cell growth on both Hep3B and HepJ5 cells. (hindawi.com)
Furthermore, we established a BCa cell model with downregulated LAPTM5, revealing a significantly delayed growth rate in the BCa cells with knockdown of LAPTM5. (spandidos-publications.com)
Moreover, cell cycle arrest at G0/G1 phase was triggered by decreased LAPTM5 as well, which could lead to delayed BCa cell growth. (spandidos-publications.com)
DIM treatment inhibited the growth of SKOV-3, TOV-21G, and OVCAR-3 ovarian cancer cells in both a dose- and time-dependent manner with effective concentrations ranging from 40 to 100 μM. (aspetjournals.org)
Growth-inhibitory effects of DIM were mediated by cell cycle arrest in G 2 /M phase in all the three cell lines. (aspetjournals.org)
By xenografting human breast cancer cells into nude mice, we found that VALD-3 significantly suppressed tumor cell growth while showing low toxicity against major organs. (researchsquare.com)
Due to the role of oncogenic transcriptional activators NFĸB and STAT3 in survivin protein expression, and APE1/Ref-1 redox activity regulating their transcriptional activity, we assessed selective inhibition of APE1/Ref-1's redox function as a novel method to halt prostate cancer cell growth and survival. (oncotarget.com)
Decreased expression of NBPF1 in neuroblastoma cell lines with loss of 1p36 heterozygosity and the marked decrease of anchorage-independent clonal growth of DLD1 colorectal carcinoma cells with induced NBPF1 expression further suggest that NBPF1 functions as tumor suppressor. (biomedcentral.com)
If pRb is mutated, the cell cycle is not arrested and the conflict between the p53 signal to stop cell growth and the Cdk signal to proliferate leads to apoptosis. (darkskiesfilm.com)
p21 mediates its various biological activities primarily by binding to and inhibiting the kinase activity of the cyclin-dependent kinases (CDKs) CDK2 and CDK1 (also known as CDC2) leading to growth arrest at specific stages in the cell cycle (FIG. 2). (darkskiesfilm.com)
Senescence, the cessation of cell division and permanent withdrawal from the cell cycle, is a process that occurs throughout the lifespan - during embryogenesis, growth and development, tissue remodeling, and in wound healing. (darkskiesfilm.com)
Animal study confirmed that β-peltatin significantly suppressed the growth of subcutaneously-implanted BxPC-3 cell xenografts. (biomedcentral.com)
We have examined nerve growth factor (NGF)-triggered signaling in two NIH3T3 cell lines exogenously expressing the NGF receptor, TrkA. (umassmed.edu)
Our aim was to determine if switching metabolism from glycolysis towards mitochondrial respiration would reduce growth preferentially in colorectal cancer cells over normal cells, and to examine the underlying mechanisms. (dcaguide.org)
Dichloroacetate reduced lactate levels in growth media and induced dephosphorylation of E1 α subunit of pyruvate dehydrogenase complex in all cell lines, but the intrinsic mitochondrial membrane potential was reduced in only cancer cells ( P =0.04). (dcaguide.org)
Pyruvate dehydrogenase kinase inhibition attenuates glycolysis and facilitates mitochondrial oxidative phosphorylation, leading to reduced growth of colorectal cancer cells but not of non-cancerous cells. (dcaguide.org)
The glycolytic phenotype offers growth advantages to cancer cells by resisting apoptosis, and facilitating tumour spread and metastasis (Yeluri et al, 2009). (dcaguide.org)
It has been reported to reduce growth of these cancer cells mainly by reducing inhibitory phosphorylation of PDH, thereby promoting mitochondrial oxidative phosphorylation and inducing apoptosis through mitochondrial, NFAT-Kv 1.5, and p53 upregulated modulator of apoptosis (PUMA)-mediated pathways. (dcaguide.org)
Andrographolide (AGP), the major phytoconstituent isolated from Andrographis paniculata was found to exhibit growth inhibition and cytotoxicity against the hormone-independent (PC-3 and DU-145) and hormone-dependent (LNCaP) prostate cancer cell lines via the microculture tetrazolium (MTT) assay. (upm.edu.my)
We show that, in the absence of p53, mdm2 confers a growth advantage to cells (i.e. "transforms" them) and can overcome a G1 cell-cycl arrest induced by p107, a member of the pRb tumour-suppressor family (Adams and Kaelin, 1995). (hal.science)
Whereas immunohistochemistry of the metastatic tumor showed a predominantly sarcomatous vimentin positive tumor that has lost E-cadherin expression, PF338 cells demonstrated biphasic growth and carried mutations in KRAS , PIK3CA , PTEN and ARID1A . (por-journal.com)
The growth rate of the cells was reduced when grown in ( significantly? (racetab.org)
As there is a substantial growth-inhibitory aftereffect of leucine deprivation, we explored whether leucine deprivation got any inhibitory influence on cell routine development. (racetab.org)
Importantly, however, this hypersusceptibility was largely reversed in the presence of T cell growth factors. (uaeu.ac.ae)

In this study, we investigated the cytotoxic and apoptotic effects of VALD-3, a Schiff base ligand synthesized from o-vanillin derivatives, on human breast cancer cells and the possible underlying mechanisms. (researchsquare.com)
The cell cycle distribution was analyzed through PI staining and flow cytometry analysis, while apoptotic cells were measured by double staining with Annexin V-FITC and PI. (biomedcentral.com)
The largest apoptotic effect was evident in metastatic LoVo cells, in which DCA induced up to a ten-fold increase in apoptotic cell counts after 48 h. (dcaguide.org)
AGP was found to induce G2/M cell cycle arrest which led to predominantly apoptotic mode of cell death. (upm.edu.my)

VALD-3 also regulated the Wnt/β-catenin signaling pathway in breast cancer cells, inhibiting the activation of downstream molecules. (researchsquare.com)
By using antisense RNA, Lck-deficient transfectants of a T helper 2 (Th2) clone have been derived and shown to have a qualitative defect in the T cell receptor signaling pathway. (uaeu.ac.ae)

This study is the first to show that VALD-3 induces apoptosis and cell cycle arrest in human breast cancer cells by suppressing Wnt/β-catenin signaling, indicating that it could be a potential drug for the treatment of breast cancer. (researchsquare.com)
Open up in another window Shape 3 Leucine deprivation induces apoptosis of human being breast tumor BAY 293 cell(A and B) MDA-MB-231 or MCF-7 cells had been incubated in charge (+leu) or leucine-deficient (?leu) moderate for 48h, accompanied by dedication of cleaved caspases inside a and annexin-V assay in B. (C and D) Nude mice. (racetab.org)
Furthermore, T44Bf selectively induces apoptosis in leukemia cell lines when compared to normal peripheral blood mononuclear cells. (tmu.edu.tw)

Furthermore, Tan IIA-induced apoptosis and mitochondrial membrane potential changes were also further demonstrated by DNA fragmentation, single-cell gel electrophoresis assay (SCGE), and flow cytometry methods. (hindawi.com)
In vitro ubiquitination assay showed a decreased p27 ubquitination in PA-treated prostate cancer cells. (iiarjournals.org)
Cell viability was determined using Cell Counting Kit-8 assay. (biomedcentral.com)
Open up in another window Shape 1 The result of leucine deprivation on cell viability(A and B) Cells had been incubated in charge BAY 293 (+leu) or leucine-deficient (?leu) moderate for indicated period, accompanied by MTT assay. (racetab.org)
The uptake of the purified MWCNTs was evaluated using fluorescence activated cell sorting (FACS), while the effects on cell fate were assessed using a 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium salt colorimetric assay, cell cycle and nanoindentation. (cdc.gov)

These data show that di-GA exhibits three distinct anticancer activities: induction of apoptosis, cell-cycle arrest and disruption of cancer cell-induced lymphendothelial disintegration. (nature.com)
Therefore, based on clinical experience as well as cytotoxic activity against multiple human cancer cells, we hypothesized that induction of apoptosis is the underlying mechanism for the treatment of psoriasis. (hindawi.com)
Our transcriptome analysis revealed in bladder cancer (BCa) tissues a significant induction of lysosomal-associated multispanning membrane protein 5 (LAPTM5), a lysosomal membrane protein preferentially expressing in immune cells and hematopoietic cells. (spandidos-publications.com)
β-peltatin initially arrested PAC cells at G2/M phase, followed by apoptosis induction. (biomedcentral.com)
Scholars@Duke publication: Inhibition of Isoprenylcysteine Carboxylmethyltransferase Induces Cell-Cycle Arrest and Apoptosis through p21 and p21-Regulated BNIP3 Induction in Pancreatic Cancer. (duke.edu)

The aim of the present investigation was to elucidate the cellular mechanisms whereby Tanshinone IIA (Tan IIA) leads to cell cycle arrest and apoptosis in vitro in keratinocytes, the target cells in psoriasis. (hindawi.com)
We investigated the effects of various concentrations of Tan IIA (5-10 μ g/mL) on mouse keratinocytes and human HaCat cells in vitro to confirm this hypothesis. (hindawi.com)
Moreover, treatment with TCEE is found to enhance the cytotoxic effects of amphotericin B in human colon cancer cell both in vitro and in vivo [ 17 ]. (hindawi.com)
Because APE1/Ref-1 is expressed and elevated in prostate cancer, we sought to characterize APE1/Ref-1 expression and activity in human prostate cancer cell lines and determine the effect of selective reduction-oxidation (redox) function inhibition on prostate cancer cells in vitro and in vivo . (oncotarget.com)
Viability assays and cell cycle analyses were used to test in vitro sensitivity to different standard and novel treatments. (por-journal.com)
In this manuscript, we report the in vitro mutagenicity of waterpipe smoke condensate (WSC), the alteration in cellular parameters of lung alveolar cells in response to WSC exposure and discuss the implication of cellular responses in the pathophysiology of chronic obstructive pulmonary disease (COPD). (who.int)

Zhao H , Halicka HD , Li J , Darzynkiewicz Z , . Berberine suppresses gero-conversion from cell cycle arrest to senescence. (aging-us.com)

Because age-related cellular senescence and type 2 diabetes (T2D) have been recognised as risk factors for CRC development, the recent finding that type 2 diabetic patients present an elevated circulating volume of senescent cells raises the question whether type 2 diabetes facilitates the process of CRC tumorigenesis by inducing premature cell senescence. (frontiersin.org)
However, WSC induced cell cycle arrest and cellular senescence mediated by the p53-p21 pathway. (who.int)

Strikingly, histone deacetylase (HDAC) inhibition markedly reduced cell viability by inducing a dose-dependent G0/1 arrest and led to mesenchymal-epithelial transition as evidenced by morphological change and increased E-cadherin and β-catenin expression. (por-journal.com)
Outcomes Leucine deprivation decreases viability of tumor cells We 1st determined the result of leucine deprivation on viability of tumor cells. (racetab.org)
Rigosertib reduced cell viability of neuroblastoma cells and caused cell cycle arrest in the G2/M phase. (lu.se)

Hematopoietic stem cell transplantation (bone marrow, cord blood, or peripheral blood stem cells) may cure aplastic anemia and prevent myelodysplastic syndrome or leukemia. (medscape.com)
The epidermis is derived primarily from surface ectoderm but is colonized by pigment-containing melanocytes of neural crest origin, antigen-processing Langerhans cells of bone marrow origin, and pressure-sensing Merkel cells of neural crest origin. (medscape.com)
Recipients of grafts containing immune cells (particularly bone marrow, intestine, and liver) are at risk of graft-vs-host disease, in which the donor immune cells attack recipient tissue. (msdmanuals.com)
B lymphocyte development is a highly ordered process pro- expression analysis without the use of intermediate amplifica- ceeding from the progenitor cells in the bone marrow (BM) to tion steps. (lu.se)

These results were further confirmed in Chk2 knockout DT40 lymphoma cells, in which DIM failed to cause cell cycle arrest. (aspetjournals.org)

Four varieties of tumor cells were examined: malignant melanoma A375, lung caner A549, ovarian carcinoma breast and A2780 cancer MCF-7 and MDA-MB-231 cells. (racetab.org)
Towards elucidating the effects of purified MWCNTs on human lung epithelial cells. (cdc.gov)
Herein, we examined the potential of purified MWCNTs used at occupationally relevant exposure doses for particles not otherwise regulated to affect human lung epithelial cells. (cdc.gov)
Living donors are often used for kidney and hematopoietic stem cell transplants, less frequently for segmental liver transplants, and rarely for pancreas and lung transplants. (msdmanuals.com)

The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
An option to eliminate the negative effects of chronic senescent cells is to kill them specifically, using compounds called senolytics (Figure 2), which target pathways activated in senescent cells [16]. (darkskiesfilm.com)
NF-κB pathways are associated with immunity, inflammation and cell survival. (springer.com)
Akt pathways have effects on apoptosis, protein synthesis, metabolism and cell cycle. (springer.com)

It results in a single layer of arrested cells of arrested cells, and is a process that is notably missing in cancer cells. (wikipedia.org)
Furthermore, di-GA inhibited the generation of lymphendothelial gaps by cancer cell spheroid-secreted lipoxygenase metabolites. (nature.com)
The ethanolic extract of T. camphoratus (TCEE) which contains abundant bioactive compounds including triterpenoids and polysaccharides also has antitumor effects in various human cancer cell lines. (hindawi.com)
In this study, by comparing the activity of normal cell lines and cancer cell lines after treating with resveratrol, it was found that resveratrol has more significant cytotoxicity in cancer cell lines. (mdpi.com)
Resveratrol could play a toxic role through inducing apoptosis of the cancer cell in a time- and concentration-dependent manner. (mdpi.com)
We evaluated the effect of 3,3′-diindolylmethane (DIM) in ovarian cancer cells. (aspetjournals.org)
These results clearly indicate the requirement of Chk2 activation to cause G 2 /M arrest by DIM in ovarian cancer cells. (aspetjournals.org)
Our results establish Chk2 as a potent molecular target of DIM in ovarian cancer cells and provide the rationale for further clinical investigation of DIM. (aspetjournals.org)
Flow cytometry analysis showed that VALD-3 triggered cell cycle arrest and induced apoptosis of breast cancer cells. (researchsquare.com)
We have demonstrated that phenylacetate(PA)-induced cell cycle arrest in human prostate cancer is mediated by increase of p27. (iiarjournals.org)
In this study, we further investigated the mechanism of PA-induced p27 expression in prostate cancer cells (LNCaP, androgen-independent LNCaP [AIDL] and PC-3). (iiarjournals.org)
A striking decrease in Skp2 mRNA and protein expression and reciprocal increase in p27 protein level were observed in three PA-treated prostate cancer cells. (iiarjournals.org)
Our results suggest that PA attenuated Skp2 expression, thereby inhibiting ubiquitination and promoting p27 accumulation in all three prostate cancer cell lines. (iiarjournals.org)
LC-MS/MS analysis was used to investigate the proteome of DLD1 colon cancer cells with induced NBPF1 expression. (biomedcentral.com)
Here we show that methanolic extracts of Sapota fruit (MESF) induces cytotoxicity in a dose-dependent manner in cancer cell lines. (nature.com)
Annexin V-propidium iodide double-staining demonstrated that Sapota fruit extracts potentiate apoptosis rather than necrosis in cancer cells. (nature.com)
In a preliminary study it has been shown that phenolic antioxidants such as methyl 4- O -galloylchlorogenate and 4- O -galloylchlorogenic acid derived from Sapota fruits can induce cytotoxicity in colon cancer cells 23 . (nature.com)
Cancer cells are highly dependent on glycolysis. (dcaguide.org)
Representative colorectal cancer and non-cancerous cell lines were treated with dichloroacetate (DCA), an inhibitor of pyruvate dehydrogenase kinase. (dcaguide.org)
Early observations concerning the metabolic differences between cancer and normal cells were made by Otto Warburg, who showed that cancer cells are inherently dependent on glycolysis for production of chemical energy (Warburg, 1956). (dcaguide.org)
This study aimed to examine the antitumour effects and possible fundamental mechanisms of aspirin in pancreatic cancer cells . (bvsalud.org)
Andrographolide induces cell cycle arrest and apoptosis in PC-3 prostate cancer cells. (upm.edu.my)
Here, we show that ASncmtRNA knockdown induces cell death preceded by proliferative blockage in three different human breast cancer cell lines. (uandes.cl)
In the pres ent study, the inhibitory effect of the root extract of ASH (ASHE) on HuH ‑ 7 and HepG2 liver cancer cells was examined. (lab-sunchlorella.com)
Fluoresce nce and transmission electron micrographs of ASHE ‑ treated liver cancer cells showed the presence of an increased number of autophagic vesicles. (lab-sunchlorella.com)
In conclusion, ASHE exerts cytostat ic activity on liver cancer cells via both apoptosis and autophagy, and may serve as a potential therapeutic agent for management of liver cancer and autophagy ‑ related diseases. (lab-sunchlorella.com)
Apoferritin-encapsulation of cysteine protease inhibitors for cathepsin L inhibition in cancer cells. (nottingham.ac.uk)

Given that some of the mechanisms below of inducing cell cycle arrest involve damaging the DNA, this allows investigation into how the cell responds to damage of its genetic material. (wikipedia.org)
The underlying molecular mechanisms by which androgen-independent PC cells acquire the capacity to proliferate remain largely unclear. (go.jp)
This work adds to the elucidation of the molecular mechanisms behind cell cycle arrest preceding tumor cell apoptosis induced by ASncmtRNA knockdown. (uandes.cl)
The purpose of BAY 293 our current research would be to investigate the result of leucine deprivation on breast tumor cells and also to elucidate the root mechanisms. (racetab.org)

In fact, several studies have shown that T2D induces senescence in multiple types of cells, including fibroblasts and endothelial cells ( 11 , 12 ). (frontiersin.org)

Furthermore, the combined drug effects of TCEE with conventional chemotherapy agents, cisplatin and doxorubicin, were also analyzed to clarify whether TCEE enhances or antagonizes the cytotoxicity of the selected chemotherapy agents in hepatocellular carcinoma cells. (hindawi.com)
Cell cycle-dependent cytotoxicity, G2/M phase arrest, and disruption of p34cdc2/cyclin B1 activity induced by doxorubicin in synchronized P388 cells. (aspetjournals.org)
Our results suggest that anthracycline-induced cytotoxicity is cell cycle dependent and is mediated, at least in part, by disturbance of the regulation of p34cdc2/cyclin B1 complex, thus leading to G2/M phase arrest. (aspetjournals.org)
BAY 293 We looked into the cytotoxicity of leucine deprivation on regular cells further, including major hepatocytes, brownish adipose cells (BAT) and MCF-10A cells. (racetab.org)

Cell Cycle Arrest and microRNA Signatures in Pancreatic Adenocarcinoma Cells. (bvsalud.org)
Effect of Aspirin on G 0 /G 1 Cell Cycle Arrest and microRNA Signatures in Pancreatic Adenocarcinoma Cells. (bvsalud.org)

How do senescent cells accumulate? (darkskiesfilm.com)
Over lifetime, due to the action of several stressors such as DNA damage and telomere shortening, senescent cells accumulate in the organism and release a variety of pro-inflammatory cytokines responsible for low-grade inflammation. (frontiersin.org)

Cycloheximide or Chk2 inhibitor pretreatment abrogated not only activation of Chk2 but also G 2 /M arrest and apoptosis mediated by DIM. (aspetjournals.org)
NGF-induced p21(WAF1) expression, cell-cycle arrest and process extension are abolished by N-nitro-L-arginine methyl ester (L-NAME), a competitive inhibitor of NOS. (umassmed.edu)

This was associated with disruption of cell cycle-related events, including hyperphosphorylation and inhibition of p34 cdc2 kinase activity, phosphorylation of cdc25C, and alteration in cyclin B1 expression. (elsevierpure.com)

Genetic engineering of cells with specific gene knockouts can also result in cells that arrest at different phases of the cell cycle. (wikipedia.org)
G2: Fission yeast expressing some mutant forms of CDC2 unable to arrest in G2 in response to DNA damage, indicating the gene product is involved in G2 arrest. (wikipedia.org)
Which gene causes cell-cycle arrest? (darkskiesfilm.com)
effects the main target for p53-induced cell cycle arrest seems to be the p21 gene. (bio2009.org)
Activation of p53 efficiently counteracts deacetylase-mediated repression and induces cell cycle arrest by activating the p21 gene. (bio2009.org)
known to be essential for autophagy) gene knockout cells. (elsevierpure.com)
immature cell is then subjected to negative selection to delete grouped based on their previously defined pheno- self-reactive cells before it leaves the BM to enter peripheral typic features, and a gene expression pattern for lymphoid organs, where it becomes a mature B cell [9]. (lu.se)

Protein activation and expression were analysed by western blotting, deoxyribonucleoside triphosphate levels by HPLC, ribonucleotide reductase activity by 14 C-cytidine incorporation into nascent DNA and cell-cycle distribution by FACS. (nature.com)
Two PTGR1-specific short hairpin RNA (shRNA) sequences were used to block the expression of PTGR1 via a lentivirus-mediated system in the androgen-independent PC cell lines DU145 and PC 3. (go.jp)
We show here that expression of EBF3 resulted in cell cycle arrest and apoptosis. (aacrjournals.org)
Additionally, forced expression of NBPF1 in two p53-mutant neuroblastoma cell lines also resulted in a G1 cell cycle arrest and CDKN1A upregulation. (biomedcentral.com)
A ) Active caspase-3 expression was detected by flow cytometry in HeLa, SiHa, and ME-180 cells after treatment with IC 25 and IC 50 of MNBE for 24 h. (biomedcentral.com)
B ) Western blot analysis of the expression of NFkB-p65 and p53 in HeLa, SiHa, and ME-180 cells treated with MNBE at indicated dose for 24 h. (biomedcentral.com)
Aspirin treatment statistically increased the expression of 274 miRNAs in PANC-1 cells and 30 miRNAs in PK-8 cells and suppressed the expression of 294 miRNAs in PANC-1 cells and 13 miRNAs in PK-8 cells . (bvsalud.org)
Mdm2 over-expression in tumours is reminiscent of p53 mutations with gain of function, in that Mdm2 both transforms cells and inhibits p53 activity. (hal.science)
In UCS, several studies identified characteristic EMT-related expression signatures including active TGF-β signaling in tissue and cell lines [ 7 , 11 , 13 , 14 ]. (por-journal.com)
In this study, we report that surface expression of CD26, through its associated DPPIV enzyme activity, enhanced sensitivity of Jurkat T-cell transfectants to G 2 -M arrest induced by the chemotherapeutic drug, doxorubicin. (elsevierpure.com)
p21 and p53 expression was also significantly lower in GCD2 cells compared to the WT. (elsevierpure.com)

Frog egg cell extracts have been used extensively in cell cycle research because they are relatively large, reaching a diameter of 1mm, and so contain large amounts of protein, making protein levels more easily measurable. (wikipedia.org)
p27Kip1 protein levels are elevated in arresting cells. (wikipedia.org)

Due to its greater cytotoxic potency and selectivity towards PC-3 cells, flow cytometry was used to analyze the cell cycle distribution of control and treated PC-3 cells whereas Annexin V-FITC/PI flow cytometry analysis was carried out to confirm apoptosis induced by AGP in PC-3 cells. (upm.edu.my)

Silencing of the EBF3 locus was observed in brain, colorectal, breast, liver, and bone tumor cell lines and its reactivation was achieved on treatment with 5-aza-2′-deoxycytidine and trichostatin A in a significant portion of these tumor cells. (aacrjournals.org)
In addition, we demonstrate that the addition of exogenous soluble DPPIV enhanced sensitivity of lymphoid tumor cell lines to doxorubicin, suggesting a potentially useful clinical role for CD26/DPPIV in the treatment of selected human hematological malignancies. (elsevierpure.com)
ASncmtRNA knockdown with an antisense oligonucleotide induces massive apoptosis in tumor cell lines, without affecting healthy cells. (uandes.cl)

G 2 /M arrest was associated with DNA damage as indicated by phosphorylation of H 2 A.X at Ser139 and activation of checkpoint kinase 2 (Chk2) in all the three cell lines. (aspetjournals.org)
These two cell lines express similar levels of TrkA and respond to NGF with rapid elevation of mitogen-activated protein kinase (MAPK) activity. (umassmed.edu)
Focusing on the extensively studied family of thiosemicarbazone (TSC) compounds, we have previously identified 4,4′-dimethoxybenzophenone thiosemicarbazone (T44Bf) as a promising pharmacological compound in a panel of human leukemia cell lines (HL60, U937, KG1a and Jurkat). (tmu.edu.tw)
each group of cell lines was established. (lu.se)
cells are activated by interaction with antigens and obtain T indicated that the cell lines representing a defined cell help, they mature into terminally differentiated plasma stage generally presented a high similarity in over- cells secreting large amounts of antibodies [10 -12]. (lu.se)
The use of cell lines does, however, introduce a risk the immunoglobulin (Ig)-secreting plasma cell in the spleen, of obtaining cell line-specific features as a result of the trans- gut, or BM [1, 2]. (lu.se)

MAPK activation is slightly more sustained for E25 cells than for TRK1 cells, although sustained activation of MAPK has been suggested to cause cell-cycle arrest. (umassmed.edu)
These results demonstrate that sustained activation of MAPK is not the sole determining factor for NGF-induced cell-cycle arrest and implicate NO in the cascade of events leading to NGF-induced morphological changes and cell-cycle arrest. (umassmed.edu)

The transfectants were significantly larger than the parental cells and contained 4N DNA. (uaeu.ac.ae)
CD95 counts in the 3 types of white blood cells were significantly higher in PEM infants and showed improvement after nutritional rehabilitation yet not reaching the control values. (who.int)

The aberrant accumulation of senescent cells with age results in potential detrimental effects. (darkskiesfilm.com)
This study concluded that senescent cell accumulation results from a combination of two effects. (darkskiesfilm.com)

In human somatic cells, the cell cycle lasts about 18 hours, and the G1 phase makes up about 1/3 of that time. (wikipedia.org)
Apoptosis was measured by Hoechst 33258/propidium iodide double staining of nuclear chromatin and the formation of gaps into the lymphendothelial barrier in a three-dimensional co-culture model consisting of MCF-7 tumour cell spheroids and human lymphendothelial monolayers. (nature.com)
The aims of this study are to clarify the antitumor effects of TCEE on human hepatocellular carcinoma cells and also evaluate the combination drug effects with conventional chemotherapy agents, cisplatin and doxorubicin. (hindawi.com)
In contrast, the antitumor effects and related biological mechanism of TCEE as well as the combination drug effects with conventional chemotherapy agents remain unclear particularly in human hepatocellular carcinoma cells. (hindawi.com)
The aims of this preclinical study are to evaluate the capability of TCEE to suppress human hepatocellular carcinoma cells and clarify the related antitumor effects. (hindawi.com)
CD26, a M r 110,000 surface-bound ectopeptidase with dipeptidyl peptidase IV (DPPIV) activity, has an array of diverse functional properties, with a role in T-cell physiology and the development of certain human cancers. (elsevierpure.com)

In a cell-free system, Dox did not inhibit p34cdc2 kinase activity, indicating that is has no direct effect on the enzyme function. (aspetjournals.org)

An intrinsic mechanism that through the secretion of a specific molecule called Wingless by the neighbouring cells, instruct the receiving cells to keep their proliferative state. (europa.eu)
Senescence is a stress-response cellular state characterised by proliferative arrest but active metabolism ( 7 ). (frontiersin.org)

LAPMT5 is a lysosomal membrane protein preferentially expressed in immune cells ( 5 , 6 ) and hematopoietic cells ( 7 ), having a close interaction with the Nedd4 ( 8 ), a member of the E3 ubiquitin ligases family ( 8 ). (spandidos-publications.com)
Hematopoietic Stem Cell Transplantation Hematopoietic stem cell (HSC) transplantation is a rapidly evolving technique that offers a potential cure for hematologic cancers ( leukemias, lymphomas, myeloma) and other hematologic disorders. (msdmanuals.com)

TRK1 cells cease to proliferate and extend long processes in response to NGF, while E25 cells continue to proliferate in the presence of NGF. (umassmed.edu)
During the fourth week of embryologic development, the single cell thick ectoderm and underlying mesoderm begin to proliferate and differentiate. (medscape.com)

In damaged cells, p53 is activated and causes cell cycle arrest by inducing p21 and by inhibiting pRb phosphorylation by Cdks. (darkskiesfilm.com)

Synthesis of folic acid functionalized gold nanoclusters for targeting folate receptor-positive cells. (nottingham.ac.uk)

What happens when a cell goes into senescence? (darkskiesfilm.com)
Loss of p53 function promotes (directly and indirectly) chromosomal instability, inducing cells to enter either senescence or apoptosis [13]. (darkskiesfilm.com)
In adult tissues, senescence is triggered primarily as a response to damage, allowing for suppression of potentially dysfunctional, transformed, or aged cells. (darkskiesfilm.com)
How do you stop a cell from senescence? (darkskiesfilm.com)

Anatomy25

Organisms13

Diseases4

Chemicals and Drugs113

Analytical, Diagnostic and Therapeutic Techniques and Equipment21

Phenomena and Processes82

Disciplines and Occupations1

Information Science3

Health Care2

Proliferation23

Progression18

Checkpoints1

Activation of apoptosis2

Proteins7

Differentiation5

Genes10

Mitotic arrest2

Induce cell2

Cyclin-dependent kinase2

Growth32

Apoptotic4

Signaling pathway2

Induces apoptosis3

Assay5

Induction5

Vitro6

Suppresses1

Cellular senescence2

Viability3

Bone marrow4

Failed to cause cell cycle arrest1

Lung4

Pathways4

Cancer27

Mechanisms4

Fibroblasts1

Cytotoxicity4

Pancreatic2

Senescent cells accumulate2

Inhibitor2

Disruption1

Gene7

Expression11

Protein levels2

Apoptosis induced1

Tumor cell lines3

Lines6

MAPK2

Significantly2

Accumulation2

Human6

Inhibit1

Proliferative2

Hematopoietic2

Proliferate2

Phosphorylation1

Receptor1

Senescence4