Arrest cell cycle progression. Medical search. Frequent questions

While knockout circIFT80 in HT29 cell or SW480 cells, the proliferation, and migration of the cells were inhibited, the cell cycle was arrested in G2/M phase, and the cell apoptosis was increased. (hindawi.com)
For example, circRNA_000166 can function as an oncogene by promoting the proliferation of CRC cells and by limiting apoptosis. (hindawi.com)
β -Catenin is a transcription factor that plays an important role in cell proliferation, cellular differentiation, apoptosis, and metastasis [ 10 ]. (hindawi.com)
Previous research has demonstrated that β -catenin can contribute to malignant progression and distant metastasis in CRC by promoting proliferation, limiting apoptosis, and accelerating epithelial-mesenchymal transition [ 11 , 12 ]. (hindawi.com)
At a fundamental level, cancer is caused by an accumulation of genetic changes that result in unregulated cell growth and proliferation. (medscape.com)
Tumor suppressor genes encode proteins that normally provide negative control of cell proliferation. (medscape.com)
PTEN encodes a protein kinase of the same name and functions as a tumor suppressor through regulation of cell proliferation. (medscape.com)
Blocks cell proliferation, causing arrest in G 1 phase of the cell cycle. (tocris.com)
Taken together, our results suggested that decreased LAPTM5 inhibited proliferation and viability, as well as induced G0/G1 cell cycle arrest possibly via deactivation of ERK1/2 and p38 in BCa cells. (spandidos-publications.com)
Furthermore, previous studies suggested that knockdown of LAPTM4B , another important subtype of the LAPTM family inhibited proliferation of hepatocellular carcinoma ( 11 ), prostate ( 12 ) and breast cancer cells ( 13 ). (spandidos-publications.com)
In each category, studies related to growth control genes, cell proliferation, apoptosis, DNA repair genes, antioxidant and drug detoxification genes, genes of drug metabolizing enzymes, tissue remodeling genes and genes of antibody-dependent cellular cytotoxicity were discussed separately. (researchsquare.com)
As activation of c-myc expression promotes cell proliferation and blocks differentiation, it may positively regulate cell growth. (wdv.com)
Downregulation of CDCA5 Can Inhibit Cell Proliferation, Migration, and Invasion, and Induce Apoptosis of Prostate Cancer Cells. (nih.gov)
It also abrogates proliferation of cancer cells by arresting them at different phases of the cell cycle and/or by inducing their apoptosis. (nih.gov)
The expression of cyclin-dependent kinase inhibitors was profoundly affected with early activation and then repression of p21 cip1/waf1 and persistent activation of both p27 kip1 and p57 kip2 , whereas genes involved in cell survival and proliferation were suppressed. (aacrjournals.org)
During the natural developmental process of Drosophila, the developing eye shows a remarkable, tight coordination between cell proliferation and the steps for cell specialisation. (europa.eu)
In addition, AKT/mTOR signaling and MAPK signaling were inhibited by JIB extract to suppress melanoma cell growth and proliferation. (medsci.org)
The results demonstrated that JIB extract combined with cisplatin enhanced the inhibition of cell growth, proliferation, and survival through the obstruction of cell cycle progression and AKT/mTOR and MAPK signaling as well as the induction of cell apoptosis. (medsci.org)
Our study demonstrates that survivin and APE1/Ref-1 are significantly higher in human prostate cancer specimens compared to noncancerous controls and that APE1/Ref-1 redox-specific inhibition with small molecule inhibitor, APX3330 and a second-generation inhibitor, APX2009, decreases prostate cancer cell proliferation and induces cell cycle arrest. (oncotarget.com)
Negative growth regulation generally occurs during the G0/G1 phase of the cell cycle, yet the redundancy and complexity among components of this regulatory network have made it difficult to discern how negative growth cues protect cells from aberrant proliferation. (uwo.ca)
Among its different constituents, Astragalus polysaccharides have been most widely studied particularly for their immunopotentiating properties, such as stimulation of murine B cell proliferation and cytokine production ( 6 ). (spandidos-publications.com)
Our results indicated that AST inhibits cell proliferation through phase-specific cell cycle arrest and caspase-dependent apoptosis. (spandidos-publications.com)
To investigate the effects of silibinin on the growth of oral cancer cells, cell proliferation and anchorage-independent colony formation tests were conducted on YD10B and Ca9-22 oral cancer cells. (jcancer.org)
Silibinin effectively suppressed YD10B and Ca9-22 cell proliferation and colony formation in a dose-dependent manner. (jcancer.org)
Results and Conclusions: The adaptation process was characterized by initial cell population growth arrest, and after that extensive cell death, followed by proliferation and long-term survival of clonal cultures. (lu.se)
Proliferation and cell cycle progression of the serum-free cultures closely mimicked that of serum-dependent cells. (lu.se)
Further screening showed increased cell proliferation. (who.int)
Inactivation of tumor suppressor genes, whose products normally provide negative control of cell proliferation, contributes to malignant transformation in various cell types. (medscape.com)

Contact inhibition is a method of arresting cells when neighboring cells come into contact with each other. (wikipedia.org)
It further inhibited cell-cycle progression in the G1 phase by four different mechanisms: rapid downregulation of cyclin D1, induction of Chk2 with simultaneous downregulation of Cdc25A, induction of the Cdk-inhibitor p21 Cip/Waf and inhibition of ribonucleotide reductase activity resulting in reduced dCTP and dTTP levels. (nature.com)
Our studies show that after being secreted into the extracellular matrix, CCN5 binds to the α6β1 integrins of the cells leading to inhibition of the PI3K-AKT signaling pathway. (ku.edu)
Chk1 facilitates the arrest of cell cycle progression and the inhibition of replication origin firing. (caltech.edu)
Because APE1/Ref-1 is expressed and elevated in prostate cancer, we sought to characterize APE1/Ref-1 expression and activity in human prostate cancer cell lines and determine the effect of selective reduction-oxidation (redox) function inhibition on prostate cancer cells in vitro and in vivo . (oncotarget.com)
Due to the role of oncogenic transcriptional activators NFĸB and STAT3 in survivin protein expression, and APE1/Ref-1 redox activity regulating their transcriptional activity, we assessed selective inhibition of APE1/Ref-1's redox function as a novel method to halt prostate cancer cell growth and survival. (oncotarget.com)
In whole cells, Dox treatment prevented p34cdc2 kinase dephosphorylation without altering its synthesis, and this effect was due to neither down-regulation of cdc25C nor inhibition of protein-tyrosine phosphatase activity. (aspetjournals.org)
In contrast, AJs found between leader cells in the same migrating sheet have cortical flows aligned in the same direction, and no such mechanical inhibition takes place. (ibecbarcelona.eu)

Analysis of the changes of migration and invasion, showed significant reduced LAPTM5 suppressed cell metastasis. (spandidos-publications.com)
Breast cancer stem cells (BCSCs) play a critical role in chemoresistance, metastasis, and poor prognosis of breast cancer. (hindawi.com)
is known to exert anticancer effects, such as inducing cell cycle arrest, inhibiting metastasis, and overcoming immunotherapy resistance in breast cancer cells. (hindawi.com)
Curcumin has been found to suppress initiation, progression, and metastasis of a variety of tumors. (nih.gov)
Our scientists pursue every aspect of cancer research-from exploring the biology of genes and cells, to developing immune-based treatments, uncovering the causes of metastasis, and more. (mskcc.org)
Angiogenesis is essential in the process of carcinogenesis to facilitate tumor progression and metastasis. (spandidos-publications.com)
Additionally, the expression and activity of matrix metalloproteinases (MMPs) are increased in almost every type of human cancer, which may be associated with cell invasion, metastasis and angiogenesis. (spandidos-publications.com)
Wound healing and Transwell tests were used to analyze the invasion and metastasis of cancer cells. (bvsalud.org)

Cellular processes like genome duplication and cell division stop. (wikipedia.org)
Aging kidney and CKD share many common characteristic features with increased cellular senescence, a conserved program characterized by an irreversible cell cycle arrest with altered transcriptome and secretome. (frontiersin.org)
Cellular senescence is characterized by an irreversible and permanent cell cycle arrest coupled with altered transcriptome and secretome. (frontiersin.org)
In contrast, in many cancerous cell lines and tumors, where growth factor dysregulation is frequently at the heart of cellular transformation, the STAT proteins (in particular Stats 1, 3 and 5) are persistently tyrosine phosphorylated or activated. (nih.gov)
while Stats 3 and 5 are involved in promoting cell cycle progression and cellular transformation and preventing apoptosis. (nih.gov)
For optimal responses, DNA repair must coordinate with other cellular processes, such as cell-cycle progression and programmed cell death. (the-scientist.com)
3 Such cellular-suicide mechanisms can eliminate cells that could present problems for the whole organism because of alterations in the DNA or difficulties in dealing with stressful stimuli. (the-scientist.com)
For example, neuronal cell death after stroke or in several neurodegenerative disorders likely occurs via programmed cell death responding to cellular stress signals. (the-scientist.com)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Most changes were subtle, but frequently the genes with altered expression were involved in basal cellular functions such as cell division, cell cycle regulation, apoptosis and cell signalling. (lu.se)
Blocking MMP-3 expression inhibited gemcitabine resistance and cancer progression in cellular and animal models. (bvsalud.org)
The term Langerhans cell histiocytosis is generally preferred to the older term, histiocytosis X. This newer name emphasizes the histogenesis of the condition by specifying the type of lesional cell and removes the connotation of the unknown ("X") because its cellular basis has now been clarified. (medscape.com)
Specifically, a variety of other cellular populations have been identified that possess phenotypic characteristics similar to Langerhans cells, including expression of CD207 and Birbeck granules. (medscape.com)
Therefore, in addition to epidermal Langerhans cells, other potential cellular origins for LCH include dermal langerin + dendritic cells, lymphoid tissue-resident langerin + dendritic cells, and monocytes that can be induced by local environmental stimuli to acquire a Langerhans cell phenotype. (medscape.com)

[1] is the differentiation of the ovum (egg cell) into a cell competent to further develop when fertilized. (wikipedia.org)
In recent years, epithelial-mesenchymal transition (EMT) has been suggested to play a key role in the process of embryonic development, differentiation of tissues and organs, chronic inflammation and fibrosis, as well as cancer progression ( 14 ). (spandidos-publications.com)
For the developer, cell differentiation is the key to cancer therapy. (cancer.gov)
Antineoplastons are the surveillance system that directs cancer cells into normal channels of differentiation . (cancer.gov)
The notion of controlling tumor growth through a naturally occurring biochemical mechanism in the body that directs cancer cells into normal channels of differentiation is one of the theoretical foundations of antineoplaston therapy. (cancer.gov)
The proposed components in the body that correct the differentiation problems of abnormal cells and send them into normal pathways have been given the name "antineoplastons" by the developer. (cancer.gov)
While some patients experience spontaneous regression or differentiation of the tumor, others are affected by rapid and fatal tumor progression despite increasingly intensive treatment strategies [ 9 ]. (biomedcentral.com)
Studies using fission yeast were instrumental in identifying fundamental mechanisms that govern cell division, differentiation, and epigenetics, to name but a few. (cshlpress.com)
Subsequent differentiation allows for rear- analysis of a set of mouse B lineage cell lines rep- rangements of the Ig light-chain (IgL) genes that replace the resenting defined stages of B cell development us- surrogate light-chain genes on the surface of the B cell [8]. (lu.se)

M: A mutant screen of budding yeasts with mitotic arrest identified CDC16, CDC23, and CDC27 as key genes that, when mutated, cause arrest in mitosis. (wikipedia.org)
2) a more complete definition of the sets of genes which are activated by different STATs and (3) how these sets of activated genes differ as a function of cell type. (nih.gov)
The intersection between the differentially expressed genes (DEGs) and the HNK-targets was determined using a Venn diagram, and the results were analyzed using a protein-protein interaction network, hub gene selection, gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses, genetic alteration analysis, survival rate, and immune cell infiltration levels. (hindawi.com)
The majority of cell cycle regulators, including APC/C, as well as the genes regulating cells' responses to external cues, are very similar between Drosophila and mammals, although the genetic circuit in Drosophila is relatively simple and less complex compared to mammals. (europa.eu)
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. (thebiogrid.org)
We investigated by real-time quantitative RT-PCR the expression profile of a panel of genes important in cell cycle regulation. (biomedcentral.com)
Targeted therapy drugs are used to target specific genes and proteins of cancer cells to precisely identify and attack specific types of cancer cells. (medsci.org)
Some alterations were restored when the cells were transferred back to serum-containing medium, indicating that expression of these genes was controlled by components in serum. (lu.se)
Like Rb protein, many of the proteins encoded by tumor suppressor genes act at specific points in the cell cycle. (medscape.com)
TP53 activates the expression of genes involved in apoptosis, cell cycle regulation (p21), and MDM2. (medscape.com)
Another important class of tumor suppressor genes involved in cell cycle control and in the generation of human cancers is the cyclin-dependent kinase (CDK) inhibitors. (medscape.com)
To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)

Replacement of wild-type RB can suppress the tumorigenicity of some of these cells, suggesting that the RB protein (Rb) may negatively regulate cell growth. (wdv.com)
Transforming growth factor (3(TGF-P) is a crucial mediator of mammary epithelial morphogenesis and can negatively regulate cell cycle progression. (uwo.ca)
In this dissertation, I explore the mechanisms that coordinate and regulate cell-cycle progression in the budding yeast, Saccharomyces cerevisiae. (duke.edu)

It results in a single layer of arrested cells of arrested cells, and is a process that is notably missing in cancer cells. (wikipedia.org)
Reprimo (RPRM), a downstream effector of p53-induced cell cycle arrest at G2/M, has been proposed as a putative tumor suppressor gene (TSG) and as a potential biomarker for non-invasive detection of gastric cancer (GC). (pucv.cl)
Due to its key role in the eukaryotic cell cycles, Plk1 has been one of the most validated drug targets for cancer treatment. (nature.com)
Furthermore, di-GA inhibited the generation of lymphendothelial gaps by cancer cell spheroid-secreted lipoxygenase metabolites. (nature.com)
These data show that di-GA exhibits three distinct anticancer activities: induction of apoptosis, cell-cycle arrest and disruption of cancer cell-induced lymphendothelial disintegration. (nature.com)
Circular RNAs (circRNAs) are a form of noncoding and closed loop RNA molecules and play vital roles in the progression of various types of cancer in humans. (hindawi.com)
Here, we used circRNA microarray sequencing technology to analyze the different circRNAs between CRC tissues and normal tissues and explore the role of circIFT80 in progression of colorectal cancer. (hindawi.com)
In this present study, we found that circIFT80 was abnormally overexpression in colorectal cancer tissues and tumor cells. (hindawi.com)
Noncoding RNAs, including circRNAs, microRNAs, and lncRNAs, have become a significant focus of research attention due to their crucial role in regulating the progression of multiple malignancies [ 5 ], including bladder cancer, gastric cancer, nasopharyngeal carcinoma, and pancreatic cancer. (hindawi.com)
In studies using animals or cells grown in labs, cinnamon has shown promise for its ability to slow cancer growth and even kill tumor cells. (webmd.com)
Our transcriptome analysis revealed in bladder cancer (BCa) tissues a significant induction of lysosomal-associated multispanning membrane protein 5 (LAPTM5), a lysosomal membrane protein preferentially expressing in immune cells and hematopoietic cells. (spandidos-publications.com)
It has been suggested that radiation sensitivity and chemosensitivity of cancer cells may play an important role in the local control of the tumor following these therapies. (researchsquare.com)
The tumor microenvironment (TME) promotes BCSC survival and cancer progression, and targeting the TME can aid in successful immunotherapy. (hindawi.com)
Chemoresistance, or the insensitivity of cancer cells to drug therapy, is a major factor in the failure of chemotherapy against breast cancer. (hindawi.com)
Breast cancer stem cells (BCSCs) are one of the main factors driving chemoresistance, thereby contributing to poor prognosis and clinical outcomes [ 3 - 5 ]. (hindawi.com)
CDCA5 promotes the progression of breast cancer and serves as a potential prognostic biomarker. (nih.gov)
Silencing oncogene cell division cycle associated 5 induces apoptosis and G1 phase arrest of non-small cell lung cancer cells via p53-p21 signaling pathway. (nih.gov)
CDCA5 promotes the progression of prostate cancer by affecting the ERK signalling pathway. (nih.gov)
Scientists working in basic, translational, and clinical cancer metabolism research are invited to join the Academy in New York on April 17th to discuss the intersection between cell signaling and metabolism. (nyas.org)
At the molecular level, abnormal cells that are potential cancer cells need to be "switched" to normal mode. (cancer.gov)
Patients with triple negative breast cancer (TNBC), where the cancer cells do not express nuclear hormone receptors and human epidermal growth factor receptor 2 (HER2), have worse survival rate compared to the patients with luminal subtypes of cancer. (ku.edu)
Lack of ER-α expression in TNBC cells or loss of ER-α activation after endocrine treatment of luminal cancers makes these breast cancer cells resistant to tamoxifen and other endocrine therapies. (ku.edu)
Collectively, these studies suggest that CCN5 restoration can be beneficial for the better management of breast cancer progression. (ku.edu)
LC-MS/MS analysis was used to investigate the proteome of DLD1 colon cancer cells with induced NBPF1 expression. (biomedcentral.com)
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay was executed for determination of effects of piperine on breast cancer cell viability. (ijpsonline.com)
Clonogenic assay was implemented for checking the effect of piperine on clonogenic potency of breast cancer cells. (ijpsonline.com)
Western blotting assay was implemented to check the levels of phosphatidylinositol 3-kinase and protein kinase B. Results revealed that piperine induced dose reliant cytotoxicity in MCF-7 human breast cancer cells. (ijpsonline.com)
Further, application of piperine on cancer cell colonies remarkably reduced the number of cell colonies to minimum. (ijpsonline.com)
Flow cytometric analysis evidenced that piperine arrested the cell cycle at growth 2/mitosis phase, hence suppressed the breast cancer progression. (ijpsonline.com)
In conclusion, the current investigation regarding anticancer effects of piperine against breast cancer revealed remarkable suppression of cancer cells. (ijpsonline.com)
A key feature of prostate cancer progression is the induction and activation of survival proteins, including the Inhibitor of Apoptosis (IAP) family member survivin. (oncotarget.com)
This miRNA functions as a tumor suppressor and dysregulation or loss of the host gene from which this miRNA is processed is associated with cancer progression in numerous cell types. (cancerindex.org)
Our previous findings demonstrated that formononetin initiates growth-inhibitory and pro-apoptotic activities in human colon cancer cells. (spandidos-publications.com)
In the present study, we aimed to further examine the potential of formononetin in controlling angiogenesis and tumor cell invasiveness in human colon cancer cells and tumor xenografts. (spandidos-publications.com)
We also discovered that the invasiveness of metastatic colon cancer cells was alleviated following drug treatment. (spandidos-publications.com)
Another important mechanism for the progression of colorectal cancer is the ability of the transformed cells to invade and traverse the basement membrane, which in turn facilitates the conversion of adenomas into carcinomas and then metastases ( 1 ). (spandidos-publications.com)
In recent years, we focused on investigating the anti-carcinogenic properties of total Astragalus saponins (AST) in human colon cancer cells and tumor xenografts. (spandidos-publications.com)
Subsequent study using multiple human cancer cell lines, including those of the colon, stomach and liver, further indicated the universal growth-inhibitory and pro-apoptotic effects of AST. (spandidos-publications.com)
The effects of silibinin on the migration and invasion of oral cancer cells were evaluated using transwell assays. (jcancer.org)
Western blotting revealed that silibinin downregulated SOD1 and SOD2 and triggered the JNK/c-Jun pathway in oral cancer cells. (jcancer.org)
Silibinin considerably reduced the development of oral cancer cells by inducing apoptosis, G 0 /G 1 arrest, ROS generation, and activation of the JNK/c-Jun pathway. (jcancer.org)
Cell viability assays and flow cytometry were applied to detect gemcitabine sensitivity and apoptosis levels in pancreatic cancer cells. (bvsalud.org)
Elevated MMP-3 expression was positively related to cancer invasion and gemcitabine resistance in PDA cells and gemcitabine resistant PDA cells. (bvsalud.org)
ROS determined the MMP-3 expression in pancreatic cancer cells. (bvsalud.org)
We examined the effect of ARID1A depletion on the gemcitabine sensitivity in pancreatic cancer cells and explored the role of RRM2 in ARID1A-mediated pancreatic cancer cells chemosensitivity to gemcitabine. (bvsalud.org)
We found that Knockout of ARID1A led to gemcitabine resistance in pancreatic cancer cells, effect of which could be reversed by RRM2, a gemcitabine resistance related gene. (bvsalud.org)
Basal cell carcinoma (BCC) is a nonmelanocytic skin cancer (ie, an epithelial tumor) that arises from basal cells (ie, small, round cells found in the lower layer of the epidermis). (medscape.com)
On the other hand, the infiltration of organs by a monoclonal population of aberrant cells, the possibility of lethal evolution, and the cancer-based modalities of successful treatment are all consistent with a neoplastic process. (medscape.com)

It is an artificial activation of naturally occurring cell cycle checkpoints, induced by exogenous stimuli controlled by an experimenter. (wikipedia.org)
Further, I show that during a wild-type cell cycle, checkpoints are responsible for arresting the bulk of periodic transcription. (duke.edu)

Oogenesis in Eukaryotic Cells. (wikipedia.org)
All somatic eukaryotic cells arrest progression through the cell cycle when their DNA is damaged, presumably because optimal repair of the damage would be a mechanistic challenge if the cell continued to replicate DNA or segregate chromosomes. (the-scientist.com)

Apoptosis was measured by Hoechst 33258/propidium iodide double staining of nuclear chromatin and the formation of gaps into the lymphendothelial barrier in a three-dimensional co-culture model consisting of MCF-7 tumour cell spheroids and human lymphendothelial monolayers. (nature.com)
Thus, the secretion of gall wasps stimulates plant cell growth and overrules homeostasis of the affected leaf area - this is similar to tumour outgrowth. (nature.com)
Inactivating mutations of the retinoblastoma gene (RB) are found in a wide variety of tumour cells. (wdv.com)
These findings were extended to explore the importance of the pRB anti- proliferative response during tumour formation and progression. (uwo.ca)

Mimosine is a plant amino acid that has been shown to reversibly inhibit progression beyond G1 phase in some human cells, including lymphoblastoid cells. (wikipedia.org)
Microinjection of Rb in G1 phase reversibly arrests cell-cycle progression. (wdv.com)

Moreover, some studies demonstrated that LAPTM5 was highly expressed in malignant B lymphomas and involved in B cell malignancies ( 10 ), involving in negative regulation of cell surface T and B cell receptor by promoting lysosome degradation ( 6 ). (spandidos-publications.com)
The working group of the Histiocyte Society divided histocytic disorders into three groups: (1) dendritic cell histiocytosis, (2) macrophage-related disorders, and (3) malignant histiocytosis. (medscape.com)

Therefore, understanding how the division of the cells, i.e. the cell cycle, is coordinated with their responses to the external clues is key to understanding the causes of the formation and progression of tumours as well as the pathology of degenerative disorders, which will ultimately help develop new therapeutic strategies to these diseases. (europa.eu)

Genetic engineering of cells with specific gene knockouts can also result in cells that arrest at different phases of the cell cycle. (wikipedia.org)
G2: Fission yeast expressing some mutant forms of CDC2 unable to arrest in G2 in response to DNA damage, indicating the gene product is involved in G2 arrest. (wikipedia.org)
The aim of this study was to evaluate the epigenetic silencing of RPRM gene by promoter methylation and its tumor suppressor function in GC cell lines. (pucv.cl)
Epigenetic silencing of RPRM gene by promoter methylation was evaluated in four GC cell lines. (pucv.cl)
The DNA- damage response gene, p53 , is an important mediator of this cell-death pathway. (the-scientist.com)
The second model proposes that a transcription factor (TF) network oscillator controls the timing of cell-cycle events, via proper timing of gene expression, including cyclins. (duke.edu)
By measuring global gene expression dynamics in cells with persistent CDK activity, I show that periodic transcription continues. (duke.edu)
For example, the TP53 gene, located on chromosome 17, encodes a 53-kd nuclear protein that functions as a cell cycle checkpoint. (medscape.com)
The TP53 gene is also capable of stimulating apoptosis of cells containing damaged DNA. (medscape.com)
immature cell is then subjected to negative selection to delete grouped based on their previously defined pheno- self-reactive cells before it leaves the BM to enter peripheral typic features, and a gene expression pattern for lymphoid organs, where it becomes a mature B cell [9]. (lu.se)

These bone metastases produce osteoblastic lesions that are associated with high morbidity and high mortality [ 8 ] and attempts at delaying this tumor progression with chemotherapeutic agents have only prolonged survival a few months. (oncotarget.com)
Arguments supporting the reactive nature of LCH include the occurrence of spontaneous remissions, the extensive elaboration of multiple cytokines by dendritic cells and T-cells (the so-called cytokine storm) in LCH lesions, and the good survival rate in patients without organ dysfunction. (medscape.com)

Involved in regulation of mitotic cell cycle. (nih.gov)
Prophase I arrest and progression to metaphase I in mouse oocytes are controlled by Emi1-dependent regulation of APC(Cdh1). (nih.gov)
and regulation of cell cycle process. (nih.gov)
Our results suggest that anthracycline-induced cytotoxicity is cell cycle dependent and is mediated, at least in part, by disturbance of the regulation of p34cdc2/cyclin B1 complex, thus leading to G2/M phase arrest. (aspetjournals.org)
Currently, two models of cell-cycle regulation have been proposed. (duke.edu)
This result fits with the second model of cell-cycle regulation. (duke.edu)
This finding adds a new layer of regulation to the second model, providing a mechanism that coordinates cell-cycle events with a TF network oscillator. (duke.edu)
Taken together, these data provide further insight into the regulation of the cell cycle. (duke.edu)

BCSCs can develop into many cell types and repopulate heterogeneous tumors following conventional chemotherapy or radiotherapy [ 4 , 6 ]. (hindawi.com)
Here, we have shown that Cysteine-rich 61-Connective Tissue Growth Factor-nephroblastoma-overexpressed 5 (CCN5) induces growth arrest of TNBC cells in-vitro and in xenograft tumors. (ku.edu)

In addition, Plk1 also prevented partial epithelial-mesenchymal transition (pEMT) of tubular epithelial cells via autophagy pathway. (nature.com)
In conclusion, our work reveals the impacts of circIFT80 as ceRNA in the progression of CRC, by which sponging miR-142, miR-568, and miR-634 enhanced the expression levels of β -catenin and activation Wnt/ β -catenin pathway. (hindawi.com)
It specifically negatively regulates the PI3K-AKT signaling pathway to induce cell cycle arrest. (medscape.com)
2 ] The living cell is an autonomous cybernetic system connected to, and receiving, information from its environment through an energy pathway and an information pathway. (cancer.gov)
It induced apoptosis, suppressed cell migration and invasion, blocked cell cycle and phosphatidylinositol 3-kinase/ protein kinase B signaling pathway. (ijpsonline.com)

Methods for the measurement of alterations in cell cycle progression and induction of apoptosis will be established. (dguv.de)
5 Conversely, p53 induction by oxidative damage may help reduce the development of atherosclerosis, perhaps by suppressing the growth or enhancing the death of cells involved in causing atherosclerotic lesions. (the-scientist.com)
This revealed increased expression of B-cell lymphoma 2 associated X and reduced expression of B-cell lymphoma 2, indicating apoptosis induction by piperine. (ijpsonline.com)
Coronavirus RNA-dependent RNA polymerase interacts with the p50 regulatory subunit of host DNA polymerase delta and plays a synergistic role with RNA helicase in the induction of DNA damage response and cell cycle arrest in S phase. (cdc.gov)

Loss of cell division cycle‑associated 5 promotes cell apoptosis by activating DNA damage response in clear cell renal cell carcinoma. (nih.gov)

Slows tumor progression in mouse xenograft models. (tocris.com)
CCN5 treatment leads to an expression of functional ER-α in the TNBC cells, both in-vitro and in xenograft models, and sensitizes these cells to tamoxifen, commonly used for endocrine therapies. (ku.edu)

In an academic research context, cell cycle arrest is typically performed in model organisms and cell extracts, such as Saccharomyces cervisiae (yeast) or Xenopus oocytes (frog eggs). (wikipedia.org)
Examples include: G1: Saccharomyces cerevisiae yeast expressing dominant mutant alleles of CDC28 arrest in G1, which indicates that CDC28 is necessary for passage beyond the G1 phase. (wikipedia.org)
S: Schizosaccharomyces pombe (fission yeast) expressing a temperature-sensitive mutant form of DNA polymerase delta (pol delta ts03) arrest in S phase. (wikipedia.org)
α-factor is a pheromone secreted by Saccharomyces cervisiae that arrests the yeast cells in G1 phase. (wikipedia.org)
The contributors describe basic methods for culturing and genetically manipulating fission yeast, synchronization strategies for probing the cell cycle, technologies for assessing proteins, metabolites, and cell wall constituents, imaging methods to visualize subcellular structures and dynamics, and protocols for investigating chromatin and nucleic acid metabolism. (cshlpress.com)

We transfected HepG2.2.15 with miR-18a mimics and CTGF siRNA, finding that upregulated miR-18a and downregulated CTGF suppress the viability and cause cell cycle arrest. (medscimonit.com)

Furthermore, we investigated the effect of HBX overexpression on miR-18a and CTGF, as well as the viability and cell cycle status of HepG2 cells. (medscimonit.com)
In addition, we found that HBX introduction downregulated miR-18a, upregulated CTGF, elevated the viability, and promoted cell cycle progression. (medscimonit.com)
Embryo morphology al ows options, the discovery of cell-free DNA in the evaluation of its growth, viability, and biological fluids has led to major advances in implantation capacity. (who.int)

Thus, targeting fibroblast cell activation and pEMT are critical strategies for attenuating fibrosis. (nature.com)
Protein activation and expression were analysed by western blotting, deoxyribonucleoside triphosphate levels by HPLC, ribonucleotide reductase activity by 14 C-cytidine incorporation into nascent DNA and cell-cycle distribution by FACS. (nature.com)
In normal cells and in animals, ligand dependent activation of the STATs is a transient process, lasting for several minutes to several hours. (nih.gov)
The importance of STAT activation to growth control in experiments using anti-sense molecules or dominant negative STAT protein encoding constructs performed in cell lines or studies in animals lacking specific STATs strongly indicate that STATs play an important role in controlling cell cycle progression and apoptosis. (nih.gov)
TGF-P has been shown to inhibit cyclin dependent kinase activity, which leads to activation of the retinoblastoma protein (pRB) and growth arrest. (uwo.ca)
Activation of p21 or p16 therefore causes cell cycle arrest. (medscape.com)

Forced expression of NBPF1 in HEK293T cells resulted in a G1 cell cycle arrest that was accompanied by upregulation of the cyclin-dependent kinase inhibitor p21 CIP1/WAF1 in a p53-dependent manner. (biomedcentral.com)

Decreased expression of NBPF1 in neuroblastoma cell lines with loss of 1p36 heterozygosity and the marked decrease of anchorage-independent clonal growth of DLD1 colorectal carcinoma cells with induced NBPF1 expression further suggest that NBPF1 functions as tumor suppressor. (biomedcentral.com)

The p16INK4A protein is a cell-cycle inhibitor that acts by inhibiting activated cyclin D:CDK4/6 complexes, which play a crucial role in the control of the cell cycle by phosphorylating Rb protein. (medscape.com)

JIB extract induced cell cycle arrest at the G 0 /G 1 phase and decreased cyclin and cdk protein expressions. (medsci.org)

Given that some of the mechanisms below of inducing cell cycle arrest involve damaging the DNA, this allows investigation into how the cell responds to damage of its genetic material. (wikipedia.org)
Therefore, there is an urgent need to identify the molecular mechanisms underlying the progression of CRC. (hindawi.com)
Further studies are now needed to elucidate the molecular mechanisms underlying circRNA action in the development and progression of cancers. (hindawi.com)
However, the molecular mechanisms of action of HNK in BCSCs, as well as its effects on the cell cycle, remain unclear. (hindawi.com)
This study aimed to explore the potential targets and molecular mechanisms of HNK on metastatic BCSC (mBCSC)-cell cycle arrest and the impact of the TME. (hindawi.com)
Thus, we must rely on the elegant mechanisms our cells have developed to repair damage. (the-scientist.com)
Single-strand breaks are easily fixed, but double-strand breaks require the complex mechanisms of nonhomologous end-joining and homologous recombination, the latter only being useful in late S, G 2 or M phases of the cell cycle, when homologous chromosomes are present in the cell. (the-scientist.com)
Understanding the regulatory mechanisms that control cell-cycle events remains to be a fundamental question in cell biology. (duke.edu)
His group studies the physical and molecular mechanisms by which cells detect and respond to mechanical signals. (ibecbarcelona.eu)
Whereas the mechanisms activating mechanotransduction are well studied, the reversibility of this process, whereby cells disassemble and reverse force-activated signalling pathways upon cessation of mechanical stimulation is far less understood. (ibecbarcelona.eu)
Determining how cells normally act to maintain genome integrity, and identifying defects in these genome maintenance mechanisms in individual disease states, is therefore vital to our understanding of how disease develops and for identifying new possibilities for intervention and treatment. (lancaster.ac.uk)

In human somatic cells, the cell cycle lasts about 18 hours, and the G1 phase makes up about 1/3 of that time. (wikipedia.org)

pRB functions as a negative regulatory transcription factor during the G1 to S phase cell cycle transition. (medscape.com)
p53 is a transcription factor whose expression is increased by DNA damage and blocks cell division at the G1 phase of the cell cycle to allow DNA repair. (medscape.com)
The CDK4-cyclinD complex normally phosphorylates the retinoblastoma protein (Rb protein), leading to release of the E2F transcription factor and cell cycle progression. (medscape.com)

Mammalian Emi2 mediates cytostatic arrest and transduces the signal for meiotic exit via Cdc20. (nih.gov)

In addition, it can stimulate apoptosis of cells containing damaged DNA. (medscape.com)

The overall goal is to determine whether chemical substances at the workplace are able to influence the cell cycle and to induce apoptosis, respectively, and to gain insight into their modes of action. (dguv.de)

Another reason synchrony is important is the control for amount of DNA content, which varies at different parts of the cell cycle based on whether DNA replication has occurred since the last round of completed mitosis and cytokinesis. (wikipedia.org)
While in G1 the cell synthesizes messenger RNA (mRNA) and proteins in preparation for subsequent steps of interphase leading to mitosis. (wikipedia.org)
Our results establish a crucial role for E4F during early embryonic cell cycles and reveal an unexpected function for E4F in mitosis. (cnrs.fr)

Frog egg cell extracts have been used extensively in cell cycle research because they are relatively large, reaching a diameter of 1mm, and so contain large amounts of protein, making protein levels more easily measurable. (wikipedia.org)
Drf1 forms a stable, active complex with Cdc7, even after replication arrest in egg extracts. (caltech.edu)
Through a combination of biochemical analysis, using purified proteins and cell-free extracts of Xenopus laevis eggs, and in vivo cell biological analysis using established human cell lines, my group is identifying key targets of ubiquitin and SUMO modification in the DNA damage response. (lancaster.ac.uk)

The focus of my scientific work is in the field of cell biology with particular emphasis on the question whether hazardous substances influence the cell cycle and apoptosis. (dguv.de)
Defining the functional relationships between proteins is critical for understanding virtually all aspects of cell biology. (thebiogrid.org)

Induced cell cycle arrest is the use of a chemical or genetic manipulation to artificially halt progression through the cell cycle. (wikipedia.org)
These remedies at best only slow, but not completely halt, CKD progression, and they are often ineffective and possess adverse side effects. (frontiersin.org)
After division, cells need to respond to external cues from the surrounding tissue and decide whether to continue dividing or to halt the cell cycle to become specialised in a task required for the proper function of the tissue/organ where they reside. (europa.eu)

The common pathological feature of CKD is the loss of tubular cells and progressive interstitial fibrosis. (nature.com)
Some studies showed that blocking cell cycle progression ameliorated fibrosis. (nature.com)
In this study, we explored the role of Plk1 in the classical kidney fibrosis model, UUO, and cultured fibroblast and renal tubular epithelial cells by using specific Plk1 inhibitors, genetic silencing, and heterozygous global knockout mice. (nature.com)

p27Kip1 protein levels are elevated in arresting cells. (wikipedia.org)
One of the critical factors for the control of the protein levels during the cell cycle is the Anaphase Promoting Complex/Cyclosome (APC/C). This complex recognises proteins with specific signatures (or degrons) and targets them for degradation via the protein destruction machinery called the proteasome. (europa.eu)

Also, we found that the CCN5-induced PI3K-AKT inactivation leads to stabilization and nuclear accumulation of P27KIP1 resulting in cell cycle arrest of TNBC cells. (ku.edu)
Flow cytometry was used to examine apoptosis, cell cycle distribution, and accumulation of reactive oxygen species (ROS). (jcancer.org)

Cell cycle-dependent cytotoxicity, G2/M phase arrest, and disruption of p34cdc2/cyclin B1 activity induced by doxorubicin in synchronized P388 cells. (aspetjournals.org)

The use of serum deprivation - partially or completely removing the serum and its nutrients - has been shown to arrest and synchronize cell cycle progression in G0 phase, for example in neonatal mammalian astrocytes and human foreskin fibroblasts. (wikipedia.org)
Mammalian oocytes are maintained in meiotic prophase arrest for a very long time-months in mice, years in humans. (wikipedia.org)
Objective: Serum is usually added to growth media when mammalian cells are cultured in vitro to supply the cells with growth factors, hormones, nutrients and trace elements. (lu.se)

Advanced head and neck squamous cell carcinoma (HNSCC) is treated with radiotherapy, chemotherapy, targeted therapy or a combination of these treatments. (researchsquare.com)
Besides, 12% of patients have second- or third-degree skin reactions that are sensitive to light, and about 18% of patients develop cutaneous squamous-cell carcinoma and keratoacanthoma or occurred at the same time [ 6 ]. (medsci.org)

These findings suggest that formononetin inhibits angiogenesis and tumor cell invasion, and thus support its use in the treatment of advanced and metastatic colon cancers. (spandidos-publications.com)

Increasing evidence indicates that senescent cells could be a promising new target for therapeutic intervention known as senotherapy, which includes depleting senescent cells, modulating SASP and restoration of senescence inhibitors. (frontiersin.org)
The identified potential therapeutic targets of HNK (PTTH) included CCND1 , SIRT2 , AURKB , VEGFA , HDAC1 , CASP9 , HSP90AA1 , and HSP90AB1 , which can potentially inhibit the cell cycle of mBCSCs. (hindawi.com)

Co-injection of c-myc, but not EJ-ras, c-fos or c-jun, inhibits the ability of Rb to arrest the cell cycle. (wdv.com)

While developmental senescence and acute senescence may positively contribute to the fine-tuning of embryogenesis and injury repair, chronic senescence, when unresolved promptly, plays a crucial role in kidney fibrogenesis and CKD progression. (frontiersin.org)

Initial observations indicate that CCN5 induces expression of P16INK4A and P19ARF, resulting in cell cycle arrest of the tumor cells. (ku.edu)
The p19ARF protein, which is encoded by the same locus as p16, also leads to cell cycle arrest by inhibiting the ability of MDM2 to inactivate TP53. (medscape.com)

Cell migration and invasion was monitored by transwell chambers assay. (ijpsonline.com)
Cell migration as well as cell invasive ability of MCF-7 cells was reduced to minimum by the application of piperine in dose dependent-manner. (ijpsonline.com)
Adherens junctions (AJs) allow cell contact to inhibit epithelial migration yet also permit epithelia to move as coherent sheets. (ibecbarcelona.eu)

Mammary-specific expression of CCN5 in HER2 overexpressing mice delays tumor progression significantly and reduces the tumor burden. (ku.edu)

Anatomy22

Organisms10

Diseases8

Chemicals and Drugs115

Analytical, Diagnostic and Therapeutic Techniques and Equipment20

Phenomena and Processes81

Disciplines and Occupations1

Information Science3

Health Care1

Proliferation28

Inhibition8

Metastasis8

Cellular14

Differentiation9

Genes12

Regulate3

Cancer48

Checkpoints2

Eukaryotic Cells2

Tumour4

Reversibly2

Malignant2

Formation and progression1

Gene10

Survival2

Regulation8

Tumors2

Pathway5

Induction4

Promotes1

Xenograft models2

Yeast5

Suppress1

Viability3

Activation6

Cyclin-dependent kinase1

Tumor suppressor1

Inhibitor1

Decreased cyclin1

Mechanisms11

Somatic1

Transcription factor3

Cytostatic1

Apoptosis of cells contain1

Induce apoptosis1

Mitosis3

Extracts3

Biology2

Halt3

Fibrosis3

Protein levels2

Accumulation2

Disruption1

Mammalian3

Squamous cell carc2

Cancers1

Therapeutic2

Inhibits1

Crucial role1

P19ARF2

Migration3

Significantly1