Array comparative genomic. Medical search. Frequent questions

Microarray-based comparative genomic hybridization is a method of molecular analysis that identifies chromosomal anomalies (or copy number variants) that correlate with clinical phenotypes. (nih.gov)
Another goal was to test the association between a positive microarray-based comparative genomic hybridization result and the severity of intellectual disability/developmental delay. (nih.gov)
Microarray-based comparative genomic hybridization identified structural chromosomal alterations responsible for the intellectual disability/developmental delay phenotype in 16% of our sample. (nih.gov)
We want to emphasize the need to conduct microarray-based comparative genomic hybridization on all individuals with intellectual disability/developmental delay, regardless of the severity, because the degree of intellectual disability/developmental delay does not predict the diagnostic yield of microarray-based comparative genomic hybridization. (nih.gov)
To detect CNVs, we used a set of NimbleGen whole-genome comparative genomic hybridization arrays containing 718,256 oligonucleotide probes with a median probe spacing of 500 bp. (biomedcentral.com)
We detected CNVs in rice by array-based comparative genomic hybridization. (biomedcentral.com)
CNVs can be detected using cytogenetic techniques such as fluorescent in situ hybridization, array-based comparative genomic hybridization, and SNP genotyping arrays. (biomedcentral.com)
Array-based comparative genomic hybridization characterization of cytogenetically polyclonal myeloid malignancies. (lu.se)
Array comparative genomic hybridization analysis of colorectal cancer cell lines and primary carcinomas. (ox.ac.uk)
Array comparative genomic hybridization, with a genome-wide resolution of approximately 1 Mb, has been used to investigate copy number changes in 48 colorectal cancer (CRC) cell lines and 37 primary CRCs. (ox.ac.uk)
Array comparative genomic hybridization in patient one and patient two revealed copy-number variant (CNV) deletions, respectively, ~ 1.45 Mb in size involving FOXF1 and an ~ 0.7 Mb in size involving FOXF1 enhancer and leaving FOXF1 intact. (researchgate.net)
Recently, we developed our own array-based comparative genomic hybridization (array CGH) with an average resolution of 1.3 Mb. (elsevierpure.com)
Array comparative genomic hybridization (aCGH) provides a method to quantitatively measure the changes of DNA copy number with an extremely high resolution and to map them directly onto the complete linear genome sequences. (korea.ac.kr)
To further validate the gain of chromosome 11p region that was identified by array CGH, fluorescence in situ hybridization (FISH) was performed. (korea.ac.kr)
Sung, JS, Park, KH & Kim, YH 2010, ' Genomic alterations of chromosome region 11p as predictive marker by array comparative genomic hybridization in lung adenocarcinoma patients ', Cancer Genetics and Cytogenetics , vol. 198, no. 1, pp. 27-34. (korea.ac.kr)
Karyotype and array comparative genomic hybridization were normal. (cdc.gov)
Recently, a tool called array comparative genomic hybridization was applied to the analysis of ENBs. (medscape.com)
Genetic alterations in ovarian tumors analyzed by array comparative genomic hybridization. (stanford.edu)
Using the 270 K Nimblegen comparative genomic hybridization (CGH) array on a set of cv. (usda.gov)
For generating a comparable rhesus macaque CNV dataset, we designed a rhesus macaque-specific array comparative genomic hybridization (aCGH) platform containing 950,843 unique 60-mer oligonucleotide probes. (biomedcentral.com)
Routine G-banded karyotype, array-based comparative genomic hybridization, and fluorescence in situ hybridization analyses were used to characterize the cytogenetic abnormality in the patient's bone marrow. (hindawi.com)
This case study reports a rare double minute chromosome via FLT3 amplification in CMML by using array-based comparative genomic hybridization and fluorescence in situ hybridization analyses. (hindawi.com)
Array comparative genomic hybridization (CGH) was performed following the standard protocol provided by Agilent Technologies (Agilent Technologies, Santa Clara, CA, United States). (hindawi.com)
Using quantitative PCR assays, the test gauges large deletions and duplications with BRCA1/2 and confirms the results by array comparative genomic hybridization. (genomeweb.com)
Genomic complexity (GC) and del(17p) status were assessed by array comparative genomic hybridization. (confex.com)
Comparative genomic hybridization (aCGH) is a powerful diagnostic tool for detecting DNA copy number gains and losses associated with chromosome abnormalities. (enzolifesciences.com)
DNA microarrays using comparative genomic hybridization (CGH) are frequently used for CNV screening. (bio-rad.com)
The gold standard for CNV detection is array comparative genomic hybridization (aCGH) using long oligonucleotide microarray platforms. (mlo-online.com)
hybridization, or array-structured comparative genomic hybridization. (bioskinrevive.com)
2003). More recently, array comparative genomic hybridization (CGH) analyses indicate that genomic loss at the Ptch1 locus was the fourth most commonly detected change among the tumor suppressor genes identified in the study, occurring in 19 percent of human breast cancers and 33 percent of breast cancer cell lines (Naylor et al. (bcm.edu)
To identify the deletion, we conducted gene dosage analysis and array-based comparative genomic hybridization (aCGH) analysis. (neurology.org)
Detection of small copy number variations (CNVs) in autism spectrum disorder (ASD) by custom array comparative genomic hybridization (aCGH). (bvsalud.org)
Array-based comparative genomic hybridization in early-stage mycosis fungoides: recurrent deletion of tumor suppressor genes BCL7A, SMAC/DIABLO, and RHOF. (medscape.com)
The women were randomly assigned to undergo embryo selection for transfer on the basis of day-5 morphology or PGD-A using array comparative genomic hybridization (aCGH) for 24-chromosome testing before the start of treatment. (medscape.com)

In this study, we used aCGH to compare genomic alterations in fresh-frozen lung cancer tissues of 21 adenocarcinomas (AdCCs) (11 early relapse and 10 nonrelapse) and identified genomic alterations that showed significant by different frequency between early relapse and nonrelapse AdCCs. (korea.ac.kr)
Genomic alterations of chromosome 11p region in AdCC patients were observed with aCGH, and a relapsable marker was identified in the nonrelapse group. (korea.ac.kr)
aCGH provides an understanding of genetic disorders, cancers and other genomic aberrations. (enzolifesciences.com)
Streamlining both CNV and SNP analysis of the same sample, microarray platforms are now available that incorporate SNP probes onto the aCGH array. (mlo-online.com)
Designed specifically for molecular cytogenetics research, these new arrays enable the effortless integration of SNP analysis into existing aCGH workflows, providing additional insight into underlying disease mechanisms, at little extra cost. (mlo-online.com)

The samples were divided for analysis according to the type of genomic instability that they exhibit, microsatellite instability (MSI) or chromosomal instability (CIN). (ox.ac.uk)
Because the exact genomic position is known, aberrations can also be mapped directly onto the chromosomal location. (mlo-online.com)

Integrative analysis of array-comparative genomic hybridisation and matched gene expression profiling data reveals novel genes with prognostic significance in oesophageal adenocarcinoma. (amsterdamumc.org)
Array comparative genomic hybridisation results of non-syndromic children with the conotruncal heart anomaly. (cdc.gov)

We divide the field of genomics into genotyping (focused on the genome sequence), transcriptomics (focused on genomic expression) and epigenomics (focused on epigenetic regulation of genome expression). (bmj.com)
NIFA will support studies that enable the use of a diverse array of species to advance our ability to improve genome annotations that complements Functional Annotation of Animal Genomes (FANNG) efforts or understand basic biological processes related to health and disease of agriculturally important animals, as well as studies that develop novel analytical tools and resources for the comparative genomics research community. (usda.gov)
Comparative genomics of Mycobacterium avium complex reveals signatures of environment-specific adaptation and community acquisition. (cdc.gov)
Here, we used comparative genomics to illuminate key aspects of MAC biology. (cdc.gov)

Whole-genome screening creates a personal genomic database (personal genome) that can subsequently be used to deliver 'personalised medicine' to individual patients. (nature.com)
High-depth whole-genome sequencing allows genome-wide sampling of genomic variants such as one nucleotide variants (SNVs), indels, structural variants, and copy amount variants. (bioskinrevive.com)

Now in its second edition, Applying Genomic and Proteomic Microarray Technology in Drug Discovery highlights, describes, and evaluates current scientific research using microarray technology in genomic and proteomic applications. (routledge.com)
Previously, Matson was involved in the research and development of microarray technologies, detection chemistries, as well as point-of-care devices for more than 17 years while at Beckman Coulter, Inc. He participated in the National Institute of Standards and Technology's (NIST) Advanced Technology Program sponsored Genosensor Consortium and collaborated with Sir Edwin Southern on the development of an in situ oligonucleotide array synthesis platform for the corporation. (routledge.com)
If a single genomic region has an equal DNA content in the test and the normal reference sample (reflecting a normal CNV profile), the corresponding oligonucleotide on the microarray surface will emit an equal intensity of both fluorophores. (mlo-online.com)

The prevalence of CNVs throughout the general population suggests that they represent a significant proportion of total genomic variation, and it has been estimated that CNVs may affect as much as 4% to 5% of the human genome. (mlo-online.com)

Our method of genotyping deletions on chromosome 7B relied on a model-based clustering algorithm (Mclust) to accurately predict the presence or absence of a given genomic sequence in a deletion line. (usda.gov)

Ways to abrogate heterogeneity will be required to optimise the genomic classification of tumours. (springer.com)
Not only has knowledge of the genomic landscape helped inform the development of new drugs, this understanding also promises to improve risk stratification of tumours and to determine their sensitivity to systemic therapies. (springer.com)

Genome-wide homozygosity estimation from genomic data is becoming an increasingly interesting research topic. (biomedcentral.com)
The former are considerably more informative, but the latter are far more numerous across the human genome and with the advent of array-based typing technologies also economically more feasible to determine on a genome wide scale. (biomedcentral.com)
We sequenced and constructed the complete genome of an environmental strain CR1 of P. aeruginosa and performed the comparative genomic analysis. (frontiersin.org)
We identified eight new regions of genomic plasticity and a plasmid pCR1 with a VirB/D4 complex followed by trimeric auto-transporter that can induce virulence phenotype in the genome of strain CR1. (frontiersin.org)
NIFA invites application for research developing comparative approaches that can be used to understand genome structure and function and the relationship between genomic features and phenotypes. (usda.gov)

Copy number variants (CNVs), defined as losses and gains of segments of genomic DNA, are a major source of genomic variation. (biomedcentral.com)

Microdeletions are observed in 5-10% of cases and can be divided into four different types (type 1, 2, 3 and atypical) according to the size of the deletion, the genomic location of the breakpoints and the affected gene content. (bvsalud.org)

Genomic amplification of FLT3 has not been reported in hematological disease. (hindawi.com)
Genomic amplification of FLT3 has been reported in solid tumors including colorectal cancer, breast cancer, and gastric cancer [ 9 ]. (hindawi.com)
With optimized, proprietary reagents, the Enzo Life Sciences CGH Labeling Kits for oligo arrays produce high quality data using as little as 0.25 µg of genomic DNA, without a need for pre-amplification. (enzolifesciences.com)

In this array, DNA from a person is compared to a reference genotype (a person's unique combination of genes or genetic makeup) using many probes. (msdmanuals.com)
A systematic review of geographical inequities for accessing clinical genomic and genetic services for non-cancer related rare disease. (cdc.gov)

Natural killer (NK) cell lymphomas/leukemias are highly aggressive lymphoid malignancies, but little is known about their genomic alterations, and thus there is an urgent need for identification and analysis of NK cell lymphomas/leukemias. (elsevierpure.com)

Some insights into clinical risk factors and their genomic correlates have been made. (springer.com)
Reflecting the needs of clinical research laboratories, such arrays combine high numbers of CNV probes, enabling highly accurate CNV detection, with sufficient SNP probes to reliably detect larger ROH. (mlo-online.com)
Clinical Utilization, Utility, and Reimbursement for Expanded Genomic Panel Testing in Adult Oncology. (cdc.gov)
Although the clinical importance of these microorganisms continues to expand, our understanding of their genomic diversity is limited, hampering basic and translational studies alike. (cdc.gov)

Array CGH has proven to be a specific, sensitive, and fast technique, amenable to automation for high-throughput workflows. (mlo-online.com)

Unlike Myriad, however, Pathway and most other BRCA testing providers are contributing de-identified test data to ClinGen , an-NIH funded effort to build a public, annotated database of genomic variants using standardized classification methods. (genomeweb.com)
Exome sequencing will not reveal genomic variants that take place beyond your protein-coding regions like the gene regulatory areas. (bioskinrevive.com)
Different variants of FISH can be used to retrieve information on genomes from (almost) base pair to whole genomic level, as besides only second and third generation sequencing approaches can do. (frontiersin.org)

We performed an array CGH analysis for 27 NK-cell lymphoma/leukemia cases that were classified into two disease groups based on the World Health Organization Classification (10 aggressive NK-cell leukemia cases and 17 extranodal NK/T-cell [NK/T] lymphomas, nasal type). (elsevierpure.com)

The most common non-clear cell renal cancers have also undergone genomic profiling and are characterised by distinct genomic landscapes. (springer.com)

Array CGH and real-time quantitative polymerase chain reaction data were found to correspond to delineated DNA copy number changes. (korea.ac.kr)

Array CGH analyzes all chromosomes. (sdfertility.com)

The use of several different methodological approaches and hence different homozygosity measures can help to distinguish between homozygosity-by-state and homozygosity-by-descent in studies investigating the effects of genomic autozygosity on human health. (biomedcentral.com)

Multiple replicates or several probes can be needed to increase sensitivity, requiring the averaging of data obtained with multiple probes, which reduces the resolution of an individual array. (bio-rad.com)

Development and Validation of a Genomic Tool to Predict Seminal Vesicle Invasion in Adenocarcinoma of the Prostate. (cdc.gov)

Collectively, our results provide key genomic context for these emerging pathogens and will facilitate future exploration of MAC ecology, evolution, and pathogenesis. (cdc.gov)

Still, further experimentation will be required to determine the role of these genomic regions in ENB. (medscape.com)
These hotspots of primate CNV formation provide a novel perspective on divergence and selective pressures acting on these genomic regions. (biomedcentral.com)
These genomic regions appear to have an elevated likelihood of positive selection, based on nucleotide level conservation and transcriptional data. (biomedcentral.com)

Does Perioperative Testosterone Predict Post-prostatectomy Genomic Risk Score? (cdc.gov)

We identified the differences in the genomic alteration patterns of the two groups. (elsevierpure.com)

The massive efforts to systematically find and catalog single nucleotide polymorphisms (SNPs) bear witness to the conviction that small genomic changes may provide clues to the origins of such things as heart problems, obesity, and pharmacologic responses. (the-scientist.com)
Illumina array: 2.5M SNPs incl. (who.int)

Unique to this edition is a new chapter on multiplex assays that examines the development and applications of arrays across diverse platforms. (routledge.com)

It is one of the most important food crops in the world, and a model plant for genomic studies of monocots. (biomedcentral.com)

Array CGH revealed a gain of 30.5 Mb in the 16p13.3p11.2 region and a gain of 18.1 Mb in the 19p13.3p12 region. (cancerindex.org)
On the other hand, if the test sample contains a CNV in a particular genomic region, this loss or gain of DNA is revealed as a shift in the fluorescence ratio. (mlo-online.com)

Anatomy25

Organisms2

Diseases23

Chemicals and Drugs10

Analytical, Diagnostic and Therapeutic Techniques and Equipment36

Psychiatry and Psychology4

Phenomena and Processes89

Disciplines and Occupations5

Information Science9

Named Groups1

Health Care5

Hybridization34

ACGH5

Chromosomal2

Hybridisation2

Genomics4

Whole-genome2

Microarray3

Total genomic1

Chromosome1

Tumours2

Genome5

CNVs1

Deletion1

Amplification3

Genetic2

Alterations1

Clinical4

High throughput1

Variants3

Analysis1

Cancers1

Delineated DNA copy1

Chromosomes1

Approaches1

Probes1

Adenocarcinoma1

Results1

Regions3

Predict1

Differences1

SNPs2

Platforms1

Studies1

Region2