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  • polycyclic
  • Polyhalogenated monocyclic and polycyclic aromatics are important and widely dispersed pollutants that are difficult to treat due to their occurrence in the environment at low absolute but toxicologically relevant concentrations. (asm.org)
  • aliphatic
  • Numerous microorganisms belonging to the domain Bacteria and Archaea have been isolated and their phylogeny and metabolic capacity to degrade a variety of aliphatic and aromatic hydrocarbons in varying salinities have been demonstrated. (frontiersin.org)
  • metabolism
  • strain RW1 activates dibenzo- p -dioxin and dibenzofuran for further metabolism by introducing two atoms of oxygen at a pair of vicinal carbon atoms, one of which is involved in one of the bridges between the two aromatic rings, i.e., an angular dioxygenation. (asm.org)
  • Moreover, in the presence of naproxen activity of gentisate 1,2-dioxygenase, enzyme engaged in 4-hydroxybenzoate metabolism, was completely inhibited. (pjmonline.org)
  • This product then undergoes ring cleavage to produce a linear molecule which is ultimately degraded to intermediates of central metabolism, such as succinate, pyruvate, and carbon dioxide. (wikipedia.org)
  • substrate
  • In this work we engineered the enzyme by site-saturation mutagenesis of active site amino acids to increase its activity and to broaden its substrate scope. (frontiersin.org)
  • Using EPR and Mössbauer spectroscopy, we have been able to characterize the ferric and ferrous substrate free and substrate bound states of the enzyme. (cmu.edu)
  • In general, the relevant redox functions of manganese in enzymes come into play largely with oxygen as the substrate or product. (ethz.ch)
  • carbon
  • Moreover, whereas expression of dxnA1A2 is modulated according to the available carbon source, expression of the dbfB gene encoding the ring cleavage enzyme of the dibenzofuran pathway, which is located in the neighborhood of dxnA1A2 but oriented in the opposite direction, is constitutive. (asm.org)
  • pollutants
  • Our hypothesis is that some of the enzymes have been quickly evolved after exposure to the environmental pollutants that did not previously exit in nature and they can be improved. (wsu.edu)
  • The improved enzymes can be introduced back into microorganisms to enhance their ability to degrade environmental pollutants. (wsu.edu)
  • microorganisms
  • To our surprise, we did not find Cr(OH) 3 precipitates but soluble organo-Cr(III) complexes after chromate reduction by microorganisms, enzymes, or reducing chemicals. (wsu.edu)
  • reductive
  • Azo-reductases, found in bacteria, catalyse the degradation of azo dyes through the reductive cleavage of azo groups (-N=N-). In presence of an electron donor, azo dyes are transformed into colourless aromatic amines (Zimmermann et al. (springeropen.com)
  • styrene
  • Recently, a novel biocatalytic oxidative alkene cleavage activity on styrene derivatives was identified in TM1459 from Thermotoga maritima . (frontiersin.org)
  • TM1459 was found active in the oxidative cleavage of styrene derivatives, working under mild conditions and using organic hydroperoxide and molecular oxygen as oxidant pair. (frontiersin.org)
  • organisms
  • Functional genomic studies have been useful in establishing that even organisms harboring high numbers of homologous enzymes seem to contain few examples of true redundancy. (wikipedia.org)
  • mechanisms
  • Our future research include protein engineering to make more efficient enzymes for catalysis, guided by structures and reaction mechanisms. (wsu.edu)
  • For this reason, there is an emerging need to recognize mechanisms of its degradation and enzymes engaged in this process. (pjmonline.org)
  • To gain insights into the mechanisms underlying symmetrical strand cleavages by RuvC, we performed crystallographic and biochemical analyses of RuvC from Thermus thermophilus (T.th. (biomedsearch.com)
  • manganese
  • Enzyme Commission (EC) 1.11.1.and manganese peroxidase (EC 1.11.1.13) have been intensively studied ( 3 ). (pnas.org)
  • bacteria
  • Additional examples of these enzymes, which were previously known in only iron-containing forms, have since been found in other bacteria (reviewed by Que and Reynolds, 2000 ). (ethz.ch)
  • thermophilus
  • In addition to the exclusively FADH2-dependent monooxygenase found in species such as E. coli and T. thermophilus, a version of the enzyme which can use either reduced FAD or reduced FMN has been described in Acinetobacter baumannii. (wikipedia.org)
  • different
  • Different metabolic routes have been discovered for catabolizing ferulate, (4-hydroxy-3-methoxycinnamic acid), which differs from p -coumarate by an additional methoxyl group on the aromatic ring. (mcponline.org)
  • metabolic
  • Deficiency of the enzyme 21-hydroxylase is the most common steroid-related in-born metabolic disorder, but treatments are available for this condition, called congenital adrenal hyperplasia . (citizendium.org)