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  • Arginine
  • Effect of arginase II on L-arginine depletion and cell growth in murine cell lines of renal cell carcinoma. (nih.gov)
  • Arginase I-producing myeloid derived suppressor cells have been shown to inhibit T-cell function by the depletion of L-arginine. (nih.gov)
  • Therefore, we studied in murine renal cell carcinoma (RCC) cell lines, the effect of arginase II on tumor cell proliferation and L-arginine depletion. (nih.gov)
  • Our results show that mRCC cell lines express only arginase II and were able to deplete L-arginine from the medium. (nih.gov)
  • nor-NOHA significantly (P = 0.01) reduced arginase II activity and suppressed cell growth in cells exhibiting high arginase activity.The depletion of L-arginine by mRCC induced the decrease expression of CD3zeta a key element for T-cell function. (nih.gov)
  • The results of this study show for the first time that arginase II produced by RCC cell lines depletes L-arginine resulting in decreased expression of CD3zeta. (nih.gov)
  • These results indicate that RCC cell lines expressing arginase II can modulate the L-arginine metabolic pathway to regulate both cell growth and T-cell function. (nih.gov)
  • Blocking arginase may lead to a decrease in RCC cell growth and aid in restoring immune function by increasing L-arginine availability for T-cell use. (nih.gov)
  • activity
  • Cell growth was independent of the amount of arginase activity expressed by the cells. (nih.gov)
  • Arginase activity increased in this cell line over the time of the experiments. (nih.gov)
  • When 2 mM of nor-NOHA was added to the cultures, significant reduction in arginase activity occurred at 48 and 72 hours (P = 0.002 and P = 0.001 respectively) (Figure 4A). (nih.gov)
  • patients
  • Understanding the interplay between arginase II and its interaction with the immune system may provide future therapeutic benefits to treat patients with RCC. (nih.gov)