• However, there is also evidence that earlier asymmetries in serotonin distribution and ion-channel mRNA and protein localization occur in zebrafish, chicken and Xenopus development, and similar to observations of intrinsic chirality generated by the cytoskeleton leading to organ and whole organism asymmetries in Arabidopsis this itself seems to be controlled from the macromolecular level by the cytoskeleton. (wikipedia.org)
  • As a proof-of-concept, we generated Solanum tuberosum (potato) macro-chloroplast strains overexpressing the tubulin-like GTPase protein gene FtsZ1 from Arabidopsis thaliana. (abeurope.info)
  • We have now beforehand found a hyperlink between growth and a posh comprising the Arabidopsis caspase-like protease separase (additionally named EXTRA SPINDLE POLES [ESP]) and three Arabidopsis microtubule (MT)-based centromeric protein-E-like Kinesins 7 (KIN7), which belong to the so-called KIN7.3-clade (KIN7.1, KIN7.3, and KIN7.5). (keiseronlineuniversity.com)
  • 1. Identification and characterization of Arabidopsis rat mutants and molecular cloning of RAT genes (Gelvin, Citovsky, Hohn, and Ziemienowicz laboratories, with help from undergraduate students at Tuskegee University). (purdue.edu)
  • Here we show that loss of function of Physcomitrella patens PpTON1 strongly affects development of the moss gametophore, phenocopying the developmental syndrome observed in Arabidopsis ton1 mutants: mutant leafy shoots display random orientation of cell division and severe defects in cell elongation, which are correlated with absence of PPB formation and disorganization of the cortical microtubule array in interphase cells. (silverchair.com)
  • Using this algorithm, we then studied a number of cases in Arabidopsis thaliana , including several different tissues, a variety of actin-affected mutants, and cells responding to powdery mildew. (biorxiv.org)
  • Within this review, the particular organic capabilities of AtTCTP had been researched through the use of transgenic Arabidopsis vegetation overexpressing AtTCTP. (hki-272inhibitor.com)
  • We performed a methylation-sensitive amplification polymorphism analysis to search for targets of RNA-directed DNA methylation in Arabidopsis thaliana and identified several members of a gene family encoding cysteine-rich peptides (CRPs). (biomedcentral.com)
  • We show here that the biogenesis of CLEL6 and CLEL9 peptides in Arabidopsis thaliana requires a series of processing events in consecutive compartments of the secretory pathway. (elifesciences.org)
  • Cortical microtubules regulate cell expansion by determining cellulose microfibril orientation in the root apex of Arabidopsis thaliana . (biomedcentral.com)
  • MT ‐dependent MT nucleation is particularly noticeable in plant cells because it accounts for the primary source of MT generation for assembling spindle, phragmoplast, and cortical arrays when the γ‐tubulin ring complex is anchored and activated by the augmin complex. (nsf.gov)
  • E3 ligase BRUTUS (BTS), a putative iron sensor, is expressed in both root and shoot tissues in seedlings of Arabidopsis thaliana . (molcells.org)
  • As shown above, we got no correct ampicon band, even from ''tubulin'', which is a high expressed gene we used as internal control. (igem.org)
  • Whole mount tubulin immunolabeling was applied in the above roots and observations were performed by confocal microscopy. (biomedcentral.com)
  • students: aspects and Protocols( Methods in Molecular Biology) misconfigured EditionCardiac Gene science: tools and Protocols( Methods in Molecular Biology) theoretical tubulin. (senecadevelopmentne.com)
  • Anti-alpha/beta tubulin has been positively tested in the same applications and can additionally be used for Immunoprecipitation experiments. (tebubio.com)
  • FT gene is derived from Arabidopsis thaliana , a model plant. (igem.org)
  • Here, we propose an optimized method, which allows for time-resolved measurements of stomatal conductance and thus the kinetic response of the ensemble of hundreds of stomata in parallel to small molecules in intact leaves of both Arabidopsis and the grass model Brachypodium distachyon . (biomedcentral.com)
  • Strong ABA responses were resolved in time- and in a dose-dependent manner in wild-type Arabidopsis leaves, whereas the same response was not observed in leaves of the ABA-insensitive mutant open stomata 1 - 3 ( ost1 - 3 ). (biomedcentral.com)
  • Due to the variable size of stomata in Arabidopsis and the limited dynamic response of stomata in isolated epidermal strips, evaluation of the effect of small molecules on stomatal physiology has been challenging and has led in some cases to inconsistent results. (biomedcentral.com)
  • β-tubulin intron length polymorphism among forms var. (org.ua)
  • This work is an important step toward fully cataloguing the functional elements of the Arabidopsis genome. (biomedcentral.com)
  • Are there similar probes to be used as non-antibody stains of tubulin cytoskeletal elements in fixed, permeabilized cells? (tebubio.com)
  • The Arabidopsis WUSCHEL (WUS) transcription factor establishes an organizing center within the shoot meristem that is essential for specification of stem-cell identity in overlying cells. (biologists.com)
  • Thus visualizing Actin and Tubulin in fixed or living cells and detecting them in biological samples (e.g. in Western Blots) belongs to basic experimental set-ups in Cell Biology. (tebubio.com)
  • Fig. 3: Immunofluorescence image of Arabidopsis thaliana metaphase cell stained with Anti-Tubulin polyclonal antibody (027ATN02). (tebubio.com)
  • Our SIR tubulin probes are meant for live cell imaging. (tebubio.com)
  • Fig. 6: 3D-SIM microscopy image of microtubules in primary rat cortex neuron body labeled with SiR-tubulin. (tebubio.com)
  • Finally, I would like to introduce brand new tools to stain Actin and Tubulin in living cells without the need to transfect them with vectors carrying the information for fluorescently labeled Actin/Tubulin or binding proteins. (tebubio.com)
  • CIL:38855, Arabidopsis thaliana, leaf. (ucsd.edu)
  • Leaves from wildtype Arabidopsis thaliana were fixed, embedded, sectioned, reacted with antibodies to the FtsZ1-1 protein, a prokaryotic cell division protein that is a structural homolog of tubulin. (ucsd.edu)
  • In this study we performed a systematic immunolocalization screen to identify Arabidopsis thaliana A- and B-type cyclins expressed in meiosis. (prolekarniky.cz)
  • Arabidopsis thaliana KatA, KatB and katC. (embl-heidelberg.de)
  • The PC-Esterase family [ ( PUBMED:20056006 ) ] is comprised of Cas1p, the Homo sapiens C7orf58, Arabidopsis thaliana PMR5 and a group of plant freezing resistance/cold acclimatization proteins typified by Arabidopsis thaliana ESKIMO1 [ ( PUBMED:20657172 ) ( PUBMED:21408051 ) ], animal FAM55D proteins, and animal FAM113 proteins. (embl.de)
  • First, we removed the cuticle of ''Arabidopsis thaliana'''s leaves by scratching them. (igem.org)
  • FT gene is derived from Arabidopsis thaliana , a model plant. (igem.org)
  • Here, using datasets available from literature, we connect Arabidopsis thaliana flowering time in genetic backgrounds varying in upstream signalling components with the expression levels of floral pathway integrator genes in these genetic backgrounds. (peerj.com)
  • The regulation of flowering time by these various factors has been extensively studied experimentally in the plant model species Arabidopsis thaliana . (peerj.com)
  • The Arabidopsis gene encodes a plastid homolog of the mitochondrial alternative oxidase, which is associated with phytoene desaturation. (tam-receptor.com)
  • However, there is also evidence that earlier asymmetries in serotonin distribution and ion-channel mRNA and protein localization occur in zebrafish, chicken and Xenopus development, and similar to observations of intrinsic chirality generated by the cytoskeleton leading to organ and whole organism asymmetries in Arabidopsis this itself seems to be controlled from the macromolecular level by the cytoskeleton. (wikipedia.org)
  • A long-chain biotin derivative was selected for this procedure because it allows the biotin molecules to be spaced far enough away from the tubulin protein so as not to interfere with its activity, e.g., ligand binding to SPA beads or other streptavidin based reagents. (cytoskeleton.com)
  • The protein purity of the tubulin used for labeling is determined by scanning densitometry of Coomassie Blue stained protein on a 4-20% polyacrylamide gel. (cytoskeleton.com)
  • protein_coding" "Cz06g09040.t1","No alias","Chromochloris zofingiensis","Tubulin-tyrosine ligase/Tubulin polyglutamylase [Interproscan]. (ntu.edu.sg)
  • 1. TACC3 protein regulates microtubule nucleation by affecting γ-tubulin ring complexes. (nih.gov)
  • In the past decade the first Arabidopsis genes encoding cytoskeletal proteins were identified. (bioone.org)
  • Several Arabidopsis ABPs, MAPs, and IF proteins are represented by individual genes and most were represented as as small gene families. (bioone.org)
  • 5. Centrosomal proteins CG-NAP and kendrin provide microtubule nucleation sites by anchoring gamma-tubulin ring complex. (nih.gov)
  • 16. Microtubule nucleation: The waltz between γ-tubulin ring complex and associated proteins. (nih.gov)
  • Second, homologues of signature eukaryotic proteins, such as actin and tubulin that form the core of the cytoskeleton or the ubiquitin system, have been detected in diverse archaea. (catsboard.com)
  • A few dozen genes in the actin and tubulin cytoskeletal systems have been characterized thoroughly, including gene families encoding actins, profilins, actin depolymerizing factors, α-tubulins, and β-tubulins. (bioone.org)
  • The actin and tubulin cytoskeletons, the nuclear pore, the spliceosome, the proteasome and the ubiquitin signalling system are only a few of the striking examples of the organizational complexity that seems to be a 'birthright' of eukaryotic cells. (catsboard.com)
  • 6. Mitosis-specific anchoring of gamma tubulin complexes by pericentrin controls spindle organization and mitotic entry. (nih.gov)
  • 7. Drosophila melanogaster gamma-TuRC is dispensable for targeting gamma-tubulin to the centrosome and microtubule nucleation. (nih.gov)
  • 1995). It has been recent that this cloning from the Arabidopsis gene provides identified a fresh essential aspect for phytoene desaturation (Carol et al. (tam-receptor.com)
  • Body 1 Oxygen intake in isolated membranes from cells expressing the Arabidopsis gene item (PTOX) buy LDK-378 and control cells. (tam-receptor.com)
  • Genetic evidence for quinone requirement was also obtained using Arabidopsis mutants accumulating phytoene (Norris et al. (tam-receptor.com)
  • Here, we show in Arabidopsis that the AP2 σ subunit mutant ( ap2 σ ) displays various developmental defects that are similar to those of mutants defective in auxin transport and/or signaling, including single, trumpet-shaped and triple cotyledons, impaired vascular pattern, reduced vegetative growth, defective silique development and drastically reduced fertility. (biologists.com)
  • 2. Aurora A site specific TACC3 phosphorylation regulates astral microtubule assembly by stabilizing γ-tubulin ring complex. (nih.gov)
  • 8. Promiscuous Binding of Microprotein Mozart1 to γ-Tubulin Complex Mediates Specific Subcellular Targeting to Control Microtubule Array Formation. (nih.gov)
  • 12. GCP-WD mediates γ-TuRC recruitment and the geometry of microtubule nucleation in interphase arrays of Arabidopsis. (nih.gov)
  • 18. Microtubule nucleation by γ-tubulin complexes and beyond. (nih.gov)
  • Cellular and molecular biologists frequently study Arabidopsis because it grows rapidly (its entire life cycle is only 6 weeks), produces lots of seeds, and has a genome that's easy to manipulate. (nih.gov)
  • Using cloning and sequencing we compared alpha tubulin copy variation in four species of bdelloid rotifers and four species of monogonont rotifers, which are facultatively sexual and cannot survive desiccation as adults. (biomedcentral.com)
  • In common with the typical pattern for animals, monogonont rotifers contain either one or two copies of alpha tubulin, but bdelloid species contain between 11 and 13 different copies, distributed across five classes. (biomedcentral.com)
  • Alpha tubulin is conserved and normally present in low copy numbers in animals, but multiplication of alpha tubulin copies has occurred in animals adapted to extreme environments, such as cold-adapted Antarctic fish. (biomedcentral.com)
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