• The interaction of a protein called epithiospecifier protein (ESP) with myrosinase diverts the reaction toward the production of epithionitriles or nitriles depending on the glucosinolate structure. (qxmd.com)
  • The protocol has not been tested for protein import activity but is optimized for chloroplast proteome yield and purity and minimal protein degradation. (bio.net)
  • We exploit developmentally timed peroxisomal content remodeling in Arabidopsis thaliana to elucidate peroxisome-associated protein degradation. (genetics.org)
  • To identify additional components needed for peroxisome-associated matrix protein degradation, we mutagenized a line expressing GFP-ICL, which is degraded similarly to endogenous ICL, and identified p ersistent GFP-ICL fl uorescence ( pfl ) mutants. (genetics.org)
  • Three possible mechanisms for peroxisomal matrix protein degradation can be envisioned: degradation within the organelle by resident proteases, degradation of the entire organelle via autophagy, or retrotranslocation out of the organelle followed by cytosolic degradation. (genetics.org)
  • View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin , the pathway pectin degradation and in Glycan metabolism . (uniprot.org)
  • Besides being important chaperones, many Clp/Hsp100 also participate in protein degradation by associating with the proteolytic subunit ClpP to form the Clp protease complex. (diva-portal.org)
  • 14-3-3s also interact with the ETHYLENE-OVERPRODUCER1 (ETO1)/ETO1-LIKE (EOLs), a group of three functionally redundant proteins that are components of a CULLIN-3 E3 ubiquitin ligase that target a subset of the ACS proteins for rapid degradation by the 26S proteasome. (plantcell.org)
  • These studies demonstrate that regulation of ethylene biosynthesis occurs by a mechanism in which 14-3-3 proteins act through a direct interaction and stabilization of ACS and through decreasing the abundance of the ubiquitin ligases that target a subset of ACS proteins for degradation. (plantcell.org)
  • The controlled degradation of proteins by the ubiquitin-26S proteasome system is an important regulatory mechanism in eukaryotic cells. (plantcell.org)
  • In plants, the gene families encoding the elements of the ubiquitin-26S proteasome system are greatly expanded relative to their animal counterparts ( Vierstra, 2009 ), suggesting a more prominent role for ubiquitin-based protein degradation in plant cells. (plantcell.org)
  • In ethylene biosynthesis, a subset of 1-aminocyclopropane-1-carboxylate synthase ( ACS ) proteins, which catalyze the generally rate-limiting step in ethylene biosynthesis, are specifically targeted by ETHYLENE OVER-PRODUCER1 (ETO1) for ubiquitination and rapid degradation by the 26S proteasome. (plantcell.org)
  • In Arabidopsis thaliana, establishment of the circadian period relies on targeted degradation of TIMING OF CAB EXPRESSION 1 (TOC1) by the 26S proteasome. (washington.edu)
  • These results demonstrate that CUL1 is required for TOC1 degradation and further suggest that this protein is the functional cullin for the SCF(ZTL) complex. (washington.edu)
  • The A-domain of Toc159 is dispensable for its in vivo activity in Arabidopsis and prone to degradation in pea ( Pisum sativum ). (rero.ch)
  • Telomeres are specific protein-DNA complexes that protect the ends of eukaryotic chromosomes from fusion and degradation and are maintained by a specialized mechanism exerted by telomerase and telomere-binding proteins (TBPs), which are evolutionarily conserved. (ovid.com)
  • Jun activation domain-binding protein-1 (JAB1) also designated COP9 subunits 5 (COPS5) or SGN5 is a coactivator of AP-1 transcription factor that also promotes degradation of the cyclin-dependent kinase inhibitor p27Kip1. (creativebiomart.net)
  • May act as a substrate-specific adapter of an E3 ubiquitin-protein ligase complex (CUL3-RBX1-BTB) which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity). (mybiosource.com)
  • From the analysis of tryptic peptides released from the surface of Arabidopsis thylakoids, the principal phosphoproteins were identified by matrix-assisted laser desorption/ionization and electrospray ionization mass spectrometry. (diva-portal.org)
  • The level of the ETO1/EOLs in vivo plays a role in mediating ACS protein turnover, with increased levels leading to a decrease in ACS protein levels. (plantcell.org)
  • The BiFC approach was used to test whether MKK1, MKK2 and MKK6 associated with MPK4 and MPK11 in vivo in Arabidopsis. (bl.uk)
  • The same tandem mass spectrometry analysis identifies other in vivo post-translational modifications as well, such as acetylation of the N-terminus of mature protein. (diva-portal.org)
  • To investigate the effect of endospheric and rhizosphere strains (WCS714r, Sphingobium limneticum and Acidovorax delafieldii) on WT and mutants, it was discovered that WCS714r and Sphingobium limneticum and Acidovorax delafiedii strains were found to inhibit the primary root length and stimulate the lateral root formation of tomato plants, Arabidopsis WT and the mutants either in vivo or in vitro except some of endospheric and rhizosphere bacteria (Pseudomonas sp. (bibsys.no)
  • However, data from this analysis suggest a function of the heterotrimeric G-protein in basal defense mechanisms against powdery mildew fungi as well as in the integration of MAMP (microbe-associated molecular patterns) perception into downstream immune responses. (uni-koeln.de)
  • Before the discovery of RGS proteins, the duration of heterotrimeric G-protein signaling was thought to be modulated by only two factors: the intrinsic GTP hydrolysis rate of the Gα subunit and acceleration of that rate by some specialized Gα effectors (i.e., phospholipase C-beta isoforms). (wikipedia.org)
  • RGS proteins are now considered key desensitizers of heterotrimeric G protein signaling and, as such, as new drug discovery targets. (wikipedia.org)
  • Prion proteins provide the best-understood mode for protein-based molecular memory. (pnas.org)
  • Molecular chaperones mediate the correct folding and assembly of polypeptides, as well as repair damaged protein structures caused by stress, while proteases remove otherwise non-functional and potentially cytotoxic proteins. (diva-portal.org)
  • Pathogen infection or treatments with conserved pathogen-associated molecular patterns (PAMPs) such as the bacterial flagellin-derived flg22 peptide are known to activate three Arabidopsis thaliana MAPK: MPK3, MPK4, and MPK6. (unboundmedicine.com)
  • In an attempt to understand the mode of action of CKs and their molecular relationships with auxins in promoting plant cell division, we looked for Arabidopsis mutants with phenotypes which were affected by exogenously applied CKs. (asm.org)
  • In this study we investigated the direct consequences of Rb protein knockout in the Arabidopsis male germline using cytological and molecular markers. (le.ac.uk)
  • J-proteins typically act as adaptors that provide substrate specificity to heat shock protein 70 (Hsp70), a molecular chaperone. (sebbm.es)
  • En este trabajo describimos como la cochaperona J20 interacciona específicamente con DXS facilitando su correcto plegamiento por la chaperona molecular Hsp70. (sebbm.es)
  • Mediante abordajes de genética, bioquímica, y biología molecular, en los últimos años hemos descubierto varios mecanismos post-transcripcionales que permiten a los cloroplastos controlar finamente los niveles y la actividad de enzimas clave de la biosíntesis de isoprenoides. (sebbm.es)
  • In molecular biology, the BURP domain is a ~230-amino acid protein domain, which has been named for the four members of the group initially identified, BNM2, USP, RD22, and PG1beta. (wikipedia.org)
  • NAC transcription factor ORE1 and senescence-induced BIFUNCTIONAL NUCLEASE1 (BFN1) constitute a regulatory cascade in Arabidopsis. (labome.org)
  • CNB domains were first identified in the regulatory subunit of mammalian cAMP-dependent protein kinase (RI and RII). (biomedcentral.com)
  • The more dynamic plus end with fast-growing and shortening characteristics plays an important role by providing binding sites for regulatory proteins, designated plus end-tracking proteins (+TIPs) ( Akhmanova and Steinmetz, 2008 ). (biologists.org)
  • We propose that At5g39790 is a regulatory scaffold protein, persistently binding the starch granule, where it is positioned to interact by its coiled-coil domain with several potential starch metabolic enzyme binding-partners. (biomedcentral.com)
  • This foundational work by Siderovski on a new class of GPCR signaling regulators has been cited over 18,000 times according to Google Scholar and also resulted in Siderovski editing a two-volume set of Methods in Enzymology chapters devoted to these regulatory proteins. (wikipedia.org)
  • In vitro activity assays of GCHII and DHBPS with all of the three purified recombinant AtRIBA proteins and complementation of E. coli ribA and ribB mutants lacking DHBPS and GCHII expression, respectively, confirmed the loss of bifunctionality for AtRIBA2 and AtRIBA3. (mdpi.com)
  • Robust production of recombinant phosphoproteins using cell-free protein synthesis," Nature Communications , vol. 6, article 8168, 2015. (hindawi.com)
  • Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. (antibodies-online.com)
  • If you cannot find the target and/or product is not available in our catalog, please click here to contact us and request the product or submit your request for custom elisa kit production , custom recombinant protein production or custom antibody production . (mybiosource.com)
  • Custom ELISA Kits, Recombinant Proteins and Antibodies can be designed, manufactured and produced according to the researcher's specifications. (mybiosource.com)
  • These studies revealed that the Dl, D2, and CP43 proteins of the photosystem II core are phosphorylated at their N-terminal threonines (Thr), the peripheral PsbH protein is phosphorylated at Thr-2, and the mature light-harvesting polypeptides LCHII are phosphorylated at Thr-3. (diva-portal.org)
  • It has been shown that several defense responses are influenced by light, and the red/far-red light photoreceptor phytochromes (PHY) modulate plant defense responses in Arabidopsis . (scialert.net)
  • This inhibition requires functional MLO, suggesting that successful defense suppression either operates through the MLO protein or that it requires the formation of post-invasive fungal infection structures. (uni-koeln.de)
  • Important changes to the proteome were observed in response to the stress treatment, including abundance changes of proteins related to photosynthesis, as well as proteins not previously identified in relation to stress. (diva-portal.org)