• Localization, biological process, and co-expression data are used as powerful indicators for protein-protein interactions. (ugent.be)
  • De Bodt S, Proost S, Vandepoele K, Rouzé P, Van de Peer Y. Predicting protein-protein interactions in Arabidopsis thaliana through integration of orthology, gene ontology and co-expression. (ugent.be)
  • Phosphorylation-resistant site mutations in PIF7 result in increased nuclear localization and shade-induced gene expression, and consequently augment hypocotyl elongation. (elifesciences.org)
  • Blocking the interaction between PIF7 and 14-3-3 proteins or reducing the expression of 14-3-3 proteins accelerates shade-induced nuclear localization and de-phosphorylation of PIF7, and enhances the shade phenotype. (elifesciences.org)
  • Central to this investigation is an understanding of what gene products are required and expressed during a natural infection and how this expression changes over time (from initial colonization to causation of disease and spread of the pathogen to new hosts) and space (in different cells or tissues within the host). (cdc.gov)
  • Although analyses that give information on the expression of a few genes provide insight and have been responsible for a large proportion of the bacterial pathogenesis literature currently available, our ultimate goal is to understand expression changes across the whole genome. (cdc.gov)
  • However, we now have a number of methods that allow identification of genes critical for survival in a host as well as methods that allow direct measurement of gene expression during interaction with a host. (cdc.gov)
  • Two of these methods, signature-tagged mutagenesis and in vivo expression technology, do not directly measure gene expression and do not allow true genomic-scale analysis, but they have been devised to identify genes necessary for pathogens during real infections. (cdc.gov)
  • A second group of methods, which includes DNA microarrays and proteomics, have advantages that overcome the limitations implicit in signature-tagged mutagenesis and in vivo expression technology, namely, the ability to directly measure expression (gene or protein) levels on a true genome-wide scale, but their application to analysis of bacterial pathogens during real infections is still in its infancy. (cdc.gov)
  • Expression data further revealed that the legume bZIP genes were expressed constitutively or in an organ-specific, development-dependent manner playing roles in multiple seed developmental stages and tissues. (datadryad.org)
  • We also detected several key legume bZIP genes involved in drought- and salt-responses by comparing fold changes of expression values in drought-stressed or salt-stressed roots and leaves. (datadryad.org)
  • Conclusions: In summary, this genome-wide identification, characterization and expression analysis of legume bZIP genes provides valuable information for understanding the molecular functions and evolution of the legume bZIP transcription factor family, and highlights potential legume bZIP genes involved in regulating tissue development and abiotic stress responses. (datadryad.org)
  • Our method considers a variety of features including protein sequences, gene co-expression, functional association, and phylogenetic profiles. (nature.com)
  • Hydrogen peroxide does not function downstream of salicylic acid in the induction of PR protein expression. (springer.com)
  • A mutation in Arabidopsis that leads to constitutive expression of systemic acquired resistance. (springer.com)
  • GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. (genscript.com)
  • Protein expression is influenced by many factors that may vary between experiments or laboratories. (genscript.com)
  • In general, information derived from microarray databases such as Arabidopsis eFP Browser ( http://bar.utoronto.ca/efp/cgi-bin/efpWeb.cgi ) and Genevestigator Gene Chronologer ( https://www.genevestigator.com/gv/index.jsp ) support experimental data on ACBP expression obtained from investigations using Northern blot analysis and reverse-transcription-polymerase chain reaction. (aocs.org)
  • Expression of hemagglutinin-epitope tagged MPK2, MPK1, and WAK was reduced when they were coexpressed with the corresponding [sRNAs] but not when coexpressed with Arabidopsis [microRNA] miR-395, which shared no sequence similarity," they wrote in Science . (genomeweb.com)
  • Researchers have shown that the expression of the CDF5 gene is strictly regulated by the union of PIF proteins (which promote its expression) and by PRR clock proteins (which prevent its expression). (sciencedaily.com)
  • Phenotypic characterization and genetic analyses of loss-of function mutant lines revealed that AtSWC4 and AtYAF9 regulate flowering altering the expression of floral master genes. (europa.eu)
  • Molecular characterization and histone modifications analyses defined a novel regulatory mechanism of plant gene expression by H2AZ deposition. (europa.eu)
  • SLocX: predicting subcellular localization of Arabidopsis proteins leveraging gene expression data. (mpg.de)
  • Exhaustion of the chloroplast protein synthesis capacity by massive expression of a highly stable protein antibiotic. (mpg.de)
  • Plastid production of protein antibiotics against pneumonia via a new strategy for high-level expression of antimicrobial proteins. (mpg.de)
  • Gene expression profiling in susceptible interaction of grapevine with its fungal pathogen Eutypa lata: Extending MapMan ontology for grapevine. (mpg.de)
  • Identification of cis-elements conferring high levels of gene expression in non-green plastids. (mpg.de)
  • The expression analysis of both gene family members reflect their preferential seed-specific expression, especially during the mature embryo development stage. (nature.com)
  • They are key regulators of gene expression in plants and other organisms. (ncbs.res.in)
  • These tertiary sRNAs mediated robust silencing of a gene involved in stomatal closure in Himalayan accessions lacking miR158 expression. (ncbs.res.in)
  • As in the case of the G box sequence, previous [ribulose-1,5-bisphosphate carboxylase small subunit] RBCS gene expression studies with transgenic plants have failed to demonstrate a requirement for these I box sequences, although mutation of what are likely to be functionally related sequences in CAB genes does affect expression (Gidoni et al. (wikiversity.org)
  • Experiments "show that site-specific mutations in either G or I, but not GT boxes, in the context of the full rbcS-IA promoter, drastically affect the rbcS-IA promoter-dependent expression of Adh and [ β -glucuronidas] GUS reporter genes. (wikiversity.org)
  • Although the RBCS1, RBCS2 and RBCS3A promoters contain closely related cis regulatory sequences, the expression patterns of the genes are different. (wikiversity.org)
  • Expression level of RP genes and proteins in Arabidopsis roots. (biomedcentral.com)
  • In order to enhance berry coloration of bright-red grape cultivars, the effects of abscisic acid (ABA) treatment on the quantity and composition of anthocyanins as well as the expression of genes related to flavonoid biosynthesis in the berry were examined. (scirp.org)
  • Resistance accompanied the expression of PDF1.2 , an ethylene signalling marker gene that requires EIN2 for transcription in wild type but not in atmyb15, atmyb38 , and atmyb44 , suggesting a disruption of ethylene signalling in the mutants. (ias.ac.in)
  • In Arabidopsis thaliana ( Arabidopsis ) treated with the harpin protein HrpN Ea , resistance to the green peach aphid Myzus persicae , a generalist phloem-feeding insect, develops with induced expression of the AtMYB44 gene. (ias.ac.in)
  • Increasing gene dosage greatly enhances recombinant expression of aquaporins in Pichia pastoris. (lu.se)
  • The gradual conversion from endosymbiont to organelle during the course of evolution has clearly been accompanied by a dramatic reduction in genome size as the chloroplasts lost most of their genes to the nucleus. (frontiersin.org)
  • The additional information generated by whole genome studies goes far beyond that derived by characterizing in isolation more genes and gene products, because analysis of the whole genome allows complete regulatory networks to be identified and characterized. (cdc.gov)
  • Here, we developed a computational approach for detecting PPIs trained and tested on known PPIs of Arabidopsis thaliana and applied to three plants, Arabidopsis thaliana , Glycine max (soybean), and Zea mays (maize) to discover new PPIs on a genome-scale. (nature.com)
  • It is possible that the ACBP1/ACBP2 and ACBP4/ACBP5 pairs could have arisen from chromosomal duplication during evolution of the Arabidopsis genome. (aocs.org)
  • There were more reads in these libraries than in cultured B. cinerea control libraries, with sequences matching the B. cinerea genome, but not Arabidopsis or tomato genomes. (genomeweb.com)
  • Among them, 52 were derived from six retrotransposon long terminal repeats loci in the B. cinerea genome, 13 were from intergenic regions of 10 loci, and eight were mapped to five protein-coding genes. (genomeweb.com)
  • Genes encoding for these two proteins are conserved in most eukaryotes, and Arabidopsis thaliana genome presents one gene encoding for SWC4 and the two YAF9 homologs, YAF9A and YAF9B. (europa.eu)
  • The comparative genome-wide analysis and mode of evolution of KCS and ELO gene families have not been investigated in tetraploid Brassica carinata and its diploid progenitors. (nature.com)
  • When DNA methylation is removed, researchers found that they could selectively turn on previously silenced genes in the underlying genome of the plant. (newswise.com)
  • The functional repertoire of the identified interactome reveals interactions between proteins functioning in well-conserved as well as plant-specific biological processes. (ugent.be)
  • We show here that PLASTID REDOX INSENSITIVE 2 (PRIN2) and CHLOROPLAST STEM-LOOP BINDING PROTEIN 41 kDa (CSP41b), two proteins identified in plastid nucleoid preparations, are essential for proper plant embryo development. (frontiersin.org)
  • Arabidopsis thaliana, the thale cress, mouse-ear cress or arabidopsis, is a small plant from the mustard family (Brassicaceae), native to Eurasia and Africa. (wikipedia.org)
  • Arabidopsis thaliana is an annual (rarely biennial) plant, usually growing to 20-25 cm tall. (wikipedia.org)
  • In 1842, German botanist Gustav Heynhold erected the new genus Arabidopsis and placed the plant in that genus. (wikipedia.org)
  • PHH1, a novel gene from Arabidopsis thaliana that encodes a protein similar to plant blue-light photoreceptors and microbial photolyases. (oregonstate.edu)
  • Background: Plant bZIP proteins characteristically harbor a highly conserved bZIP domain with two structural features: a DNA-binding basic region and a leucine (Leu) zipper dimerization region. (datadryad.org)
  • The short-term protein trafficking inhibition and long-term effect on plant growth and survival caused by TE1 were fully reversible upon drug washout. (portlandpress.com)
  • Surprisingly, except for Arabidopsis thaliana , virtually no other plant species have experimentally determined PPI data available. (nature.com)
  • Other representative plant species cover even less protein involved in known PPIs. (nature.com)
  • Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana. (genscript.com)
  • While LTPs are small proteins (~9 kDa), ACBPs in the model plant Arabidopsis range in size from 10 to 73 kDa. (aocs.org)
  • Our understanding of plant ACBPs is based predominantly on investigations on Brassica napus [4,5] and the model plant Arabidopsis thaliana [2,6-21]. (aocs.org)
  • Given that recombinant Arabidopsis ACBPs show preferences for different acyl-CoA esters and phospholipids in in vitro binding assays, it was hypothesized that members in the Arabidopsis ACBP family have nonoverlapping functions in the plant cell. (aocs.org)
  • Meanwhile, two plant defense marker genes, which do not contain target sites for the fungal sRNAs, were highly upregulated, leading the researchers to conclude that suppression of some but not all genes is a result of sequence-specific sRNA interaction and not cell death within infected lesions. (genomeweb.com)
  • Next, the scientists evaluated the impact the B. cinerea sRNAs had on plant immunity, generating transgenic Arabidopsis plants that ectopically expressed the three sRNAs under investigation. (genomeweb.com)
  • In order to confirm that the sRNAs suppressed their targets using their host's RNAi pathways, the investigators immunoprecipitated Argonaute 1 - the primary component of the RNA-induced silencing complex that enables RNAi - from Arabidopsis and identified the three sRNAs they have been studying in Ago1-associated fraction pulled from B. cinerea -infected plant samples but not controls. (genomeweb.com)
  • Thus, stress response studies during these growth stages reveal novel differentially regulated genes or proteins with important functions in plant stress adaptation. (intechopen.com)
  • Today's molecular biologists know well that the plant they use the most as a model, the Arabidopsis thaliana , lengthens the stem just before dawn when the days are short (winter). (sciencedaily.com)
  • At night, however, PIF proteins accumulate inside the cell and, just before dawn, promote the plant stem growth. (sciencedaily.com)
  • In the work published this week in Current Biology , the authors carried out an exhaustive analysis of the interactions between the proteins and the DNA of the Arabidopsis thaliana plant. (sciencedaily.com)
  • Infection of an Arabidopsis thaliana plant by the fungus Sclerotinia sclerotiorum . (mpg.de)
  • This fungus, which causes white mold disease, can colonize Arabidopsis in spite of the chemical defenses present using two detoxification pathways to deactivate the plant toxins. (mpg.de)
  • My research focuses on studying protein components of the plant NE. (brookes.ac.uk)
  • I'm using cell and molecular biology techniques, biochemistry as well as microscopy to characterise the plant SUN proteins. (brookes.ac.uk)
  • The plant PPR proteins are grouped mainly into the P and PLS classes. (rcsb.org)
  • By introducing a human protein into the model plant species Arabidopsis thaliana, researchers found that they could selectively activate silenced genes already present within the plant. (newswise.com)
  • With our new technique, you can modify how the genes are turned on and off in that plant without having to introduce a whole other set of genes from another parent. (newswise.com)
  • Let's use the plant already in the field and reawaken some of those silenced genes to generate trait variation. (newswise.com)
  • To turn these dormant or silenced genes on, researchers introduced a human enzyme, known as a ten-eleven translocation enzyme, to plant seedlings using specially modified bacteria as a delivery vector. (newswise.com)
  • Figuring out the best way to introduce the protein to the plant species has been a trial and error process. (newswise.com)
  • Ortrun Mittelsten Scheid, a senior research scientist and consultant at the Gregor Mendel Institute of Molecular Plant Biology, highlights the need for a scientifically-based public dialogue on GMO regulations, in response to a revised EU directive on gene technology. (oeaw.ac.at)
  • For their joint efforts in establishing Arabidopsis as a model organism for plant molecular genetics. (balzan.org)
  • We were among the first of a small group of scientists to champion the use of a small mustard species called Arabidopsis thaliana that has become the most widely used experimental plant today. (balzan.org)
  • We independently settled on Arabidopsis because preliminary experiments by George Redei and others indicated that it had the kinds of properties that were desirable in a model plant. (balzan.org)
  • First, we have used Arabidopsis to make a series of discoveries in plant development and biochemistry. (balzan.org)
  • The harpin protein HrpN Ea induces Arabidopsis resistance to the green peach aphid by activating the ethylene signalling pathway and by recruiting EIN2, an essential regulator of ethylene signalling, for a defence response in the plant. (ias.ac.in)
  • When Arabidopsis mutants previously generated by mutagenicity at the 37 genes were surveyed, 24 mutants were similar to the wild type plant while four mutants were more resistant and nine mutants were more susceptible than wild type to aphid infestation. (ias.ac.in)
  • Special GLUCAN SYNTHESIS-LIKE ( GSL ) genes and 𝛽-1,3-glucan callose play an important role in plant defence responses to attacks by phloem-feeding insects. (ias.ac.in)
  • In response to HrpN Ea , moreover, the AtMYB44 gene known to be required for repression of aphid reproduction on the plant was also required for repression of the phloem-feeding activity. (ias.ac.in)
  • The absence of significant effects on resistance suggests either that any putative AT-PHH1 DNA repair activity requires cofactors/chromophores not present in yeast or E. coli, or that AT-PHH1 encodes a blue-light/ultraviolet-A receptor rather than a DNA repair protein. (oregonstate.edu)
  • We provide a list of novel predictions including Danio rerio (zebrafish) and Arabidopsis thaliana (mouse-ear cress). (frontiersin.org)
  • Results: In this study, we have developed a computational method that exploits the existing knowledge on protein-protein interactions in diverse species through orthologous relations on the one hand, and functional association data on the other hand to predict and filter protein-protein interactions in Arabidopsis thaliana. (ugent.be)
  • We conclude that protein function is informative across species in predicting post-transcriptional miRNA regulation in living cells. (frontiersin.org)
  • Of these, 3,114 were single genes confined to a single species. (blogspot.com)
  • All gene family content is shown and individual cells refer to the target genes (per species). (ugent.be)
  • The B. oleracea being closest to Arabidopsis thaliana is considered an important Brassica species to study polyploidy 8 . (nature.com)
  • Because flowering plants evolved from a common ancestor only about 150 million years ago, all of the roughly 250,000 species of flowering plants are closely related and share a common set of genes. (balzan.org)
  • Our data suggest that CtBP/BARS-like protein function in plants is directly associated with the microtubule cytoskeleton. (nih.gov)
  • The MDO1 gene encodes an unknown protein that is conserved in a wide variety of land plants. (nih.gov)
  • Shade-intolerant plants, such as Arabidopsis thaliana , sense this reduction and initiate the shade avoidance syndrome (SAS). (elifesciences.org)
  • MicroRNAs (miRNAs) post-transcriptionally regulate genes across all animals and plants. (frontiersin.org)
  • In plants cells, lipid-transfer proteins (LTPs) and acyl-CoA-binding proteins (ACBPs) have been identified as candidates for lipid transfer within the cell [reviewed in 1,2]. (aocs.org)
  • The six Arabidopsis ACBPs are subcellularly located in various compartments based on experimental results using a variety of techniques including fluorescent protein tagging in confocal microscopy, immunoelectron microscopy and western blot analysis of subcellular fractions from wild-type plants as well as from transgenic plants expressing fluorescent-tagged ACBPs. (aocs.org)
  • The team discovered that 73 of the B. cinerea sRNAs could target host genes in both of the plants studied "under stringent target prediction criteria," the scientists noted. (genomeweb.com)
  • Although the plants showed normal morphology and development in the absence of pathogen challenge, even though the target genes were suppressed, all three displayed "enhanced susceptibility" to infection upon introduction of B. cinerea . (genomeweb.com)
  • Meanwhile, tomato plants in which MAPKKK4 was suppressed using a virus-induced gene silencing approach showed enhanced disease susceptibility in response to B. cinerea and contained over 15 times more fungal biomass than control plants. (genomeweb.com)
  • Further, the scientists observed a reduced disease susceptibility in Arabidopsis plants in which Ago1 was mutated following B. cinerea inoculation versus wild-type plants, while a B. cinerea mutant that cannot produce the sRNAs under investigation showed reduced pathogenicity on both Arabidopsis and tomato. (genomeweb.com)
  • This revealed that the Sax protein of the white mold fungus is active against a range of isothiocyanates, allowing it to colonize different plants of the cabbage family. (mpg.de)
  • Mid-SUN proteins are a neglected family of conserved type III membrane proteins of ancient origin with representatives in plants, animals and fungi. (brookes.ac.uk)
  • Pentatricopeptide repeat (PPR) proteins are sequence-specific RNA-binding proteins that form a pervasive family of proteins conserved in yeast, plants, and humans. (rcsb.org)
  • 1991), but to date no I-box binding protein has been cloned from plants. (wikiversity.org)
  • The main aim of our research is to understand the molecular details and physiological roles of the multitude of water channel-like proteins present in plants and animals. (lu.se)
  • Annotation of Selaginella moellendorffii major intrinsic proteins and the evolution of the protein family in terrestrial plants. (lu.se)
  • Clinal variation in the non-acclimated and cold-acclimated freezing tolerance of Arabidopsis thaliana accessions. (mpg.de)
  • Indian Himalayan natural Arabidopsis thaliana accessions with abolished miR158 levels exhibit robust miR173-initiated trans-acting cascade silencing. (ncbs.res.in)
  • Here we show that in Himalayan Arabidopsis thaliana accessions having natural mutations in miR158 locus exhibit robust cascade silencing in pentatricopeptide (PPR)-like locus. (ncbs.res.in)
  • The AT-PHH1 and SA-PHR1 cDNA sequences predict 89% identity at the protein level, except for an AT-PHH1 C-terminal extension (111 amino acids), also not seen in microbial photolyases. (oregonstate.edu)
  • This file includes the bZIPs protein sequences from Arabidopsis thaliana and six legume genomes (Glycine max,Medicago truncatula,Cajanus cajan,Cicer arietinum,Phaseolus vulgaris and Lotus japonicus). (datadryad.org)
  • The following EXO70E1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). (genscript.com)
  • These sequences represent the protein coding region of the EXO70E1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. (genscript.com)
  • In Arabidopsis , the shade-induced de-phosphorylation of the transcription factor PIF7 (PHYTOCHROME-INTERACTING FACTOR 7) is the key event linking light perception to stem elongation. (elifesciences.org)
  • These strategies involve rearrangements at the molecular level starting from transcription, regulation of mRNA processing, translation, and protein modification or its turnover. (intechopen.com)
  • The role of SUN3 in the ER is reinforced by the identification of a protein interaction between SUN3 and the ER membrane-bound transcription factor maMYB. (brookes.ac.uk)
  • String-based protein-protein interaction analysis suggested that bZIP53, a transcription factor might be involved in the activation of transcription of ELO / KCS genes. (nature.com)
  • This lack of transcription could be due to a mutation in the RBCS3A promoter creating the so-called F-box, a protein binding site located between the activating cis elements, the I-box and G-box. (wikiversity.org)
  • one includes structural genes encoding enzymes that directly participate in the formation of anthocyanins and other flavonoids, and the other comprises regulatory genes controlling the transcription of structural genes. (scirp.org)
  • We investigated 37 ethylene-inducible Arabidopsis transcription factor genes for their effects on the activation of ethylene signalling and insect defence. (ias.ac.in)
  • Twenty-eight of the 37 genes responded to both ethylene and HrpN Ea , and showed either increased or inhibited transcription, while 18 genes showed increased transcription not only by ethylene but also by HrpN Ea . (ias.ac.in)
  • In response to HrpN Ea , transcription levels of 22 genes increased, with AtMYB44 being the most inducible, six genes had decreased transcript levels, and nine remained unchanged. (ias.ac.in)
  • The prin2.2 and csp41b-2 single mutants displayed pale phenotypes, abnormal chloroplasts with reduced transcript levels of photosynthesis genes and defects in embryo development. (frontiersin.org)
  • Additionally, it can knock out genes, facilitating efforts to elucidate gene functions through the analysis of mutant phenotypes. (mdpi.com)
  • Here we report that an Arabidopsis mutant defective in At3g03940 and At5g18190, encoding closely related Ser/Thr protein kinases, shows pleiotropic phenotypes including dwarfism and hypersensitivity to osmotic/salt stress. (nih.gov)
  • Over time U-triangle developed on polyploidization of Brassica 's proved its usefulness in studying the evolution of various genes and phenotypes 3 . (nature.com)
  • Following B. cinerea infection in Arabidopsis , transcript levels of several of the sRNAs' predicted targets were suppressed. (genomeweb.com)
  • These studies have revealed that 14-3-3 proteins can interact with the phosphorylated forms of their client proteins in response to certain signals, and that this binding finalizes the signaling event by enabling a change in the subcellular localization, protein stability or intrinsic enzymatic activity of the client, or by promoting an association between the client and other proteins. (elifesciences.org)
  • Here we show that the AN gene encodes a C-terminal binding proteins/brefeldin A ADP-ribosylated substrates (CtBP/BARS) related protein. (nih.gov)
  • FAE ( Fatty Acid Elongation ) like genes take part in the biosynthesis of VLCFAs, growth regulation, and stress responses, and are further comprised of KCS ( Ketoacyl-CoA synthase ) and ELO ( Elongation Defective Elongase ) sub-gene families. (nature.com)
  • Quantitative real-time PCR analysis revealed that ABA treatment around véraison resulted in the upregulation of genes encoding enzymes responsible for both general flavonoid and anthocyanin biosynthesis. (scirp.org)
  • The three other ACBPs (ACBP4, ACBP5 and ACBP6) are cytosolic proteins. (aocs.org)
  • In the study reported here, the potato cultivar Aviva was transformed with a cytosolic Cu/Zn superoxide dismutase gene from Arabidopsis thaliana using Agrobacterium-mediated gene transformation. (scielo.org.za)
  • Reduced miR158 led to increased level of its target, a pseudo-pentatricopeptide gene that is otherwise targeted by ta-siRNAs generated by miR173 cascade in other accessions. (ncbs.res.in)
  • Transcriptional regulation of aquaporins in accessions of Arabidopsis in response to drought stress. (lu.se)
  • Currently, information on these larger proteins with acyl-CoA-binding domains is available from data mining of sequenced genomes. (aocs.org)
  • Genes that enable the successful detoxification of these substances had already been described in bacteria. (mpg.de)
  • This analysis revealed that the CDF5 gene induces stem growth just before dawn. (sciencedaily.com)
  • The growth defect and the hypersensitivity to DNA-damaging agents of mdo1-1 were enhanced significantly when combined with a lesion of the ATAXIA-TELANGIECTASIA MUTATED (ATM) gene, but not of the ATM/RAD3-RELATED (ATR) gene, suggesting that the function of the MDO1 gene is closely related to that of ATM kinase. (nih.gov)
  • Rapid and transient activation of myelin basic protein kinase in tobacco leaves treated with hairpin from Erwinia amylovora . (springer.com)
  • Arabidopsis thaliana exocyst subunit exo70 family protein E1 (EXO70E1), mRNA. (genscript.com)
  • (A) Read counts and gene numbers, (B) Percentage of the small subunit of ribosomal proteins identified in Arabidopsis roots. (biomedcentral.com)
  • (C) Percentage of the large subunit of ribosomal proteins (D) Percentage of the ribosomal-related proteins. (biomedcentral.com)
  • However, unraveling large-scale protein-protein interaction maps is laborious and expensive. (ugent.be)
  • A highly reliable set of protein-protein interactions is predicted through this integrative approach making use of existing protein-protein interaction data from yeast, human, C. elegans and D. melanogaster. (ugent.be)
  • ACBP4 and ACBP5 show conservation in a domain of five kelch motifs, which also enable interaction with protein partners. (aocs.org)
  • THA8L resembles THA8 (thylakoid assembly 8), a protein that is required for the splicing of specific group II introns of genes involved in biogenesis of chloroplast thylakoid membranes. (rcsb.org)
  • This is the first work where a PPI prediction method was developed for is the first PPI prediction method applied on benchmark datasets of Arabidopsis . (nature.com)
  • While most membranous and disease related proteins are regulated by miRNAs, the G-protein coupled receptor (GPCR) family is an exception, being mostly unregulated by miRNAs. (frontiersin.org)
  • The six-membered ACBP family in Arabidopsis consists of ACBP1 to ACBP6, with ACBP6 being the smallest member (10.4 kDa) and ACBP4 the largest (73.2 kDa). (aocs.org)
  • Elena Monte, along with her team and collaborators, has discovered that other components of the same internal clock protein family -the PRR- act sequentially during the day and most of the night to suppress the action of the PIF proteins. (sciencedaily.com)
  • The RBCS3A gene of tomato belongs to a small gene family consisting of five members. (wikiversity.org)
  • In tomato, the RBCS gene family consists of five members at three chromosomal loci ( RBCS1 , RBCS2 , RBCS3A , RBCS3B and RBCS3C ). (wikiversity.org)
  • Annotation: the most common InterPro annotation(s) of the Arabidopsis members in the family. (cornell.edu)
  • Unexpected complexity of the Aquaporin gene family in the moss Physcomitrella patens. (lu.se)
  • Multiple isolations yielded only PHH1 cDNAs, and a few blue-light-receptor CRY1 (HY4) cDNAs (also similar to microbial photolyase genes), suggesting the absence of any other highly similar Arabidopsis genes. (oregonstate.edu)
  • Because the environment encountered within a living host will be quite different from the external environment, pathogens must be able to regulate the necessary genes in coordination as they move from the environment to the host and from one host niche to another. (cdc.gov)
  • The function of the acyl-CoA-binding domain in recombinant Arabidopsis ACBPs expressed in the bacterium Escherichia coli was established using site-directed mutagenesis followed by in vitro binding assays. (aocs.org)
  • Interestingly, within actively transcribed protein-coding genes, H3T3ph density was minimal in 5' genic regions, coincidental with a peak of H3K4me3 accumulation. (nih.gov)
  • Studies in recent years have shown that this elongation of the stem in the young seedlings is controlled by PIF proteins, whose cellular accumulation depends on sunlight. (sciencedaily.com)
  • Analysis of Arabidopsis natural variation in biomass accumulation and metabolism. (mpg.de)
  • The present study characterizes an inhibitor of endomembrane protein trafficking, TE1 (trafficking and endocytosis inhibitor 1/TENin1) that reduces gravitropic root bending in Arabidopsis thaliana seedlings. (portlandpress.com)
  • The first mutant in A. thaliana was documented in 1873 by Alexander Braun, describing a double flower phenotype (the mutated gene was likely Agamous, cloned and characterized in 1990). (wikipedia.org)
  • The cpr 5 mutant of Arabidopsis expresses both NPR1-dependent and NPR1 -independent resistance. (springer.com)
  • This pattern was not affected in the double mutant, implying the existence of additional H3T3 protein kinases in Arabidopsis. (nih.gov)
  • The silencing was abolished, however, when the target genes carried a synonymously mutated version of the relevant" B. cinerea sRNA. (genomeweb.com)
  • Background: Large-scale identification of the interrelationships between different components of the cell, such as the interactions between proteins, has recently gained great interest. (ugent.be)
  • Conclusion: We conclude that the integration of orthology with functional association data is adequate to predict protein-protein interactions. (ugent.be)
  • Overall, we have predicted a reliable set of protein-protein interactions suitable for further computational as well as experimental analyses. (ugent.be)
  • Protein-protein interactions (PPIs) play essential roles in many biological processes. (nature.com)
  • Identification of protein-protein interactions (PPIs) is important for understanding how proteins work together in a coordinated fashion in a cell to perform cellular functions. (nature.com)
  • Even for Arabidopsis , known PPI data cover interactions with only about 34.55% of proteins. (nature.com)
  • ACBP1 and ACBP2 each possess a C-terminal domain of ankyrin repeats, which facilitates protein-protein interactions. (aocs.org)
  • Together, these results further our understanding of the mechanism of PPR protein-RNA interactions. (rcsb.org)
  • A new eLife paper by Fred Berger uncovers the key role histone variants play in establishing chromatin states in Arabidopsis. (oeaw.ac.at)
  • Some Arabidopsis ACBPs have also been observed to bind phospholipids in filter binding assays, e.g. recombinant ACBP6 binds phosphatidylcholine (PC). (aocs.org)
  • The researchers wanted to identify the enzymes and corresponding genes underlying this detoxification mechanism. (mpg.de)
  • On the other hand, the gene expressions of enzymes involved in proanthocyanidin synthesis were drastically decreased at véraison and remained extremely low even with ABA treatment. (scirp.org)
  • The PEP enzyme recognizes the -10 and -35 cis -elements, similar to those found in bacterial promoters whereas the NEP enzyme recognizes the YRTA-motif, which can also be found upstream of several genes with PEP promoters indicating that these genes can be transcribed by both polymerases ( Pfannschmidt and Liere, 2005 ). (frontiersin.org)
  • The transcriptionally regulated genes have different roles under stress response. (intechopen.com)
  • A cDNA from Arabidopsis thaliana similar to microbial photolyase genes, and designated AT-PHH1, was isolated using a photolyase-like cDNA from Sinapsis alba (SA-PHR1) as a probe. (oregonstate.edu)
  • Comparative genomics and functional study of lipid metabolic genes in Caenorhabditis elegans. (nih.gov)
  • Transcriptome sequencing and microarray design for functional genomics in the extremophile Arabidopsis relative Thellungiella salsuginea (Eutrema salsugineum). (mpg.de)
  • Moreover, PRIN2 and CSP41b form a distinct protein complex in vitro that binds DNA. (frontiersin.org)
  • 2011). Imidazole-grafted chitosan-mediated gene delivery: In vitro study on transfection, intracellular trafficking and degradation . (up.pt)
  • 100 ml of sheared chromatin was diluted in 900 ml ChIP dilution buffer and precleared, using 5 ml of protein A Dynabeads (Invitrogen, Carlsbad, CA). Protein A beads were removed on a magnet, and the precleared chromatin was incubated overnight at 4 °C with antibodies against either mono-methylated H3K4 (10 mL, Abcam ab8895), dimethylated H3K4 (6 mL, Upstate 07-212), or trimethylated H3K4 (6 mL, Abcam ab8580). (nih.gov)
  • Next the antibody/chromatin complex was precipitated by incubating the mix with 40 ml protein A beads for 1 hour followed by collection of the beads on a magnet. (nih.gov)
  • Therefore, to understand the interplay between SWR1-C and NuA4-C in flowering time, we studied in detail the Arabidopsis SWC4 and YAF9 genes using a combination of molecular genetics, chromatin biology and biochemical approaches. (europa.eu)
  • A PPI network provides crucial information on how biological pathways are structured and coordinated from individual protein functions. (nature.com)
  • PPIs are essential for individual protein functions, forming various cellular pathways, and are also involved in the development of diseases. (nature.com)
  • Gene structure analysis indicated that the maximum number of genes were intron-less and remained conserved during evolution. (nature.com)
  • The presence of biotic and abiotic stress-related cis-regulatory elements in the promoter region suggests that both KCS and ELO genes might also play their role in stress tolerance. (nature.com)
  • The current study provides a basis to understand the evolution of both KCS and ELO genes in fatty acid elongation and their role in stress tolerance. (nature.com)
  • Thus, the sRNAs "evidently hijacked host RNAi machinery by loading into Ago1," with the resulting complex suppressing host immunity genes, the researchers wrote. (genomeweb.com)
  • Introducing this human protein allows researchers to remove DNA methylation and thereby turn on previously silenced genes. (newswise.com)
  • Furthermore, some KCS and ELO genes were found to be specifically expressed under heat stress, phosphorus starvation, and Xanthomonas campestris infection. (nature.com)
  • Alternative transcripts of the RPS4 resistance gene in Arabidopsis: Do they produce truncated proteins? (umsystem.edu)