Aqueous buffered solution. Medical search. Frequent questions

Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol. (antikoerper-online.de)
buffer pH 7, 0.01 M: aqueous solution of 0.01 M potassium di-hydrogenphosphate adjusted to pH 7 using 1 N sodium hydroxide. (europa.eu)
buffer pH 6: buffer pH 7, 0.01 M adjusted to pH 6 using 2 M hydrochloric acid. (europa.eu)
A 10-milliliter aliquot of an aqueous solution 0.1 molar in 2-amino-2-(hydroxymethyl)-1,3- propanediol (Tris) 0.01 molar in copper-chloride and pH 5.6 to 6.1 was equilibrated with an equal volume of the MIBK solution of TSC, which contained the equivalent of five cigarettes. (cdc.gov)
All solutions were buffered (0.01 M cacodilate) and the pH was adjusted to 6.27. (bvsalud.org)

Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide. (fishersci.com)
Liquid Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. (avivasysbio.com)
Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. (gentaur.com)

Formaldehyde solution is a colorless, aqueous liquid with a pungent odor. (labce.com)
A 4% buffered aqueous formaldehyde solution is referred to as 10% neutral buffered formalin (NBF) . (labce.com)

16. Z. Luz and S. Meiboom, Rate and mechanism of proton exchange in aqueous solutions of phosphate buffer. (weizmann.ac.il)

Fluoride reactivity with dental enamel may be modified by the solution pH 11-13 or interaction with other components of the formulation, such as antibacterial agents 14 or detergents 15-16 . (bvsalud.org)

Formalin 10% buffered is the most widely used fixative for routine processing of tissue in histology laboratories. (thomassci.com)
The term "formalin" is used to describe a saturated solution of formaldehyde dissolved in water, typically with another agent (most commonly methanol) added to stabilize the solution. (labce.com)

Gallium nitrate injection is a clear, colorless, odorless, sterile solution of gallium nitrate, a hydrated nitrate salt of the group IIIa element, gallium. (nih.gov)

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl 2 , 10 mM glycine, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide. (caslab.com)

Additional concentrated lysis buffer for use with our Renilla Luciferase Assay Kits in large volume assays. (biotium.com)
Two hundred µl of DNA lysis buffer were added. (who.int)

The solution may contain sodium hydroxide or hydrochloric acid for pH adjustment to 6.0-7.0. (nih.gov)

13. Z. Luz and S. Meiboom, Proton relaxation in dilute solution of cobalt(II) and nickel(II) ions in methanol and the rate of methanol exchange of the solvation sphere. (weizmann.ac.il)
Solutions of the unretained substance and of the reference substances were prepared in 55/45 (v/v) methanol/buffer pH 6. (europa.eu)
A 5000 mg/L stock solution of the test substance was prepared in methanol. (europa.eu)
The stock solution was diluted to obtain an end solution of 55/45 (v/v) methanol/buffer pH 6. (europa.eu)
Blank solution (55/45 (v/v) methanol/buffer pH 6) was analysed by single injection. (europa.eu)

10. Z. Luz and S. Meiboom, Kinetics of proton exchange in aqueous solutions of acetate buffer. (weizmann.ac.il)

NP-EGTA AM can serve as a photolabile buffer in cells because, once converted to NP-EGTA by intracellular esterases, it will bind Ca 2+ with high affinity until photolyzed with UV light. (thermofisher.com)

A mixture containing one or more proteins of interest is dissolved in 100% buffer A and pumped into the column. (wikipedia.org)
The proteins of interest bind to the resin while other components are carried out in the buffer. (wikipedia.org)
A glass frit at the bottom of the column retains the resin beads in the column while allowing the buffer and dissolved proteins to exit. (wikipedia.org)
Water soluble proteins are extracted in a buffer solution and then precipitated to concentrate them and also to separate them from water soluble substances that may interfere with the determination. (cdc.gov)

Citrullination of antithrombin by PADI4 was analyzed by ELISA using alkaline phosphatase conjugated goat anti-rabbit IgG as the secondary diluted at 1:5000 in 0.05M carbonate/bicarbonate buffer (Ph 9.6). (caslab.com)

Cells may be prepared, stained with antibodies and washed twice with wash buffer per established protocols for immunofluorescent staining prior to acquisition on a flow cytometer. (bdbiosciences.com)

For example, blood has an average pH of ~7.4, maintained by a buffer system involving carbonic acid (H 2 CO 3 ), bicarbonate ions (HCO 3 - ), and carbon dioxide (CO 2 ). (jove.com)
Bicarbonate is also used to buffer chyme as it moves from the stomach to the small intestine. (jove.com)

The erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. (bdbiosciences.com)

The distribution of copper (7440508) between a buffered aqueous solution of the metal ion and an organic solution of tobacco smoke condensate (TSC) in 4-methyl-2-pentanone (MIBK) has been studied. (cdc.gov)
Methyl and propylparaben in a buffered solution do not enhance fluoride uptake by demineralized dental enamel. (bvsalud.org)
In QENS experiments of protein solutions hydrogens act as reporters for the motions of methyl groups or amino acids to which they are bound. (lu.se)

4. Z. Luz and B.L. Silver, The acid catalyzed exchange of phosphorus bonded hydrogen in aqueous solutions of dialkyl phosphonates studied by nuclear magnetic resonances. (weizmann.ac.il)

In contrast to HPLC, the buffer pressure used is relatively low, typically less than 5 bar, but the flow rate is relatively high, typically 1-5 ml/min. (wikipedia.org)

The potential of hydrogen (pH) is a measure of the acidity or basicity of a water-based solution determined by the concentration of hydronium ions (H 3 O + ). In one liter of pure water at neutral pH, there are 1×10 −7 moles of hydronium ions. (jove.com)
The pD dependence of the 2H relaxation in BPTI solutions could be quantitatively accounted for in terms of known p K values and hydrogen exchange rate constants. (lu.se)
A quantitative justification hydrogen isotopes 1H and 2H in aqueous protein of this point of view, however, has not been solutions has been used extensively during the past presented. (lu.se)
hydrogen atoms of the aqueous solvent. (lu.se)
Hydrogen exchange in protein solutions hydrogen atoms. (lu.se)

2 Wolbers , R., Aqueous Methods for Cleaning Painting Surfaces . (openedition.org)

Succinimidyl esters (SE) should be dissolved in a solution that is free of amine-containing compounds like Tris, glycine, or protein, which will react with the SE functional group. (biotium.com)

20. Z. Luz and R.G. Shulman, Proton magnetic resonance shifts in aqueous solutions of paramagnetic metal ions. (weizmann.ac.il)

In a common FPLC strategy, a resin is chosen that the protein of interest will bind to by a charge interaction while in buffer A (the running buffer) but become dissociated and return to solution in buffer B (the elution buffer). (wikipedia.org)
The column is a glass or plastic cylinder packed with beads of resin and filled with buffer solution. (wikipedia.org)
1 Resin and bile soaps are aqueous products, composed of water up to 95 % and are used for the cleaning of natural resin varnishes usually found on paintings. (openedition.org)
Using an aqueous system means having to deal with pH: the combination of water molecules and the adequate pH (between 5,5 and 8,5) can overcome this particular problem by playing the solubility parameters of both the resin and the dirt. (openedition.org)

The pH of pure water, 7, represents a neutral solution. (jove.com)
Buffers are critical because most biological functions occur in near-neutral pH conditions, approximately 7.35 to 7.45. (jove.com)

9. Z. Luz and S. Meiboom, Nuclear magnetic resonance study of the protolysis of trimethylammonium ion in aqueous solution-order of the reaction with respect to water. (weizmann.ac.il)
21. Z. Luz and G. Yagil, Water 17O nuclear magnetic resonance shift in aqueous solutions of 1:1 electrolyte. (weizmann.ac.il)
22. Z. Luz and B.L. Silver, The acid catalyzed oxygen exchange of acetyl-acetone in dioxane-water solution measured by oxygen-17 nuclear magnetic resonance. (weizmann.ac.il)
However, the extensive range of hydronium ion concentrations present in water-based solutions makes measuring pH in moles cumbersome. (jove.com)
Saltwater and soapy water are examples of alkaline solutions, with pHs of approximately 8 and 12, respectively. (jove.com)
PIPES [piperazine-N,N-bis(2-ethanesulfonic acid)] buffer was prepared in 18.2 megohms water and filtered through 0.22-micron filter.PH: 6.8 +/- 0.15 after dilution with DI water. (tocris.com)

Esters are not stable in aqueous solution. (biotium.com)

The injection loop is a segment of tubing of known volume which is filled with the sample solution before it is injected into the column. (wikipedia.org)

This test method covers an analytical test for determining the amount of total aqueous extractable protein associated with NR, latex, and elastomeric products. (cdc.gov)

The buffer flow rate is controlled by a positive-displacement pump and is normally kept constant, while the composition of the buffer can be varied by drawing fluids in different proportions from two or more external reservoirs. (wikipedia.org)
The generation of nanoaerosols with well-defined solution, capillary diameter, flow rate, and colloidal morphology is necessary in many applications such particle concentration on the size distributions of as instrument calibration and evaluation (Ku and SWCNT aerosols were investigated. (cdc.gov)

however, because of the wide choice of resins and buffers it has broad applications. (wikipedia.org)
It is known to destabilize nucleic acid duplexes, making it useful for preparing hybridization buffers for RNA and DNA applications. (thomassci.com)

Concentrated stock solutions should be prepared in anhydrous DMSO (see Biotium catalog no. 90082) . (biotium.com)
Stock solutions in anhydrous DMSO can be stored desiccated at -20°C for one month or longer. (biotium.com)

The blocks were exposed to the treatment solutions in the proportion of 2 ml per mm2 of exposed enamel area and fluoride formed was estimated after removing an enamel layer by acid etching. (bvsalud.org)

Recommended for use in solutions containing metal ions. (tocris.com)
When metal pipe corrosion occurs, it is a result of the electrochemical electron exchange resulting from the differential galvanic properties between metals, the ionic influences of solutions, aquatic buffering, or the solution pH. (cdc.gov)

Gallium nitrate is formed by the reaction of elemental gallium with nitric acid, followed by crystallization of the drug from the solution. (nih.gov)
24. Z. Luz and I. Pecht, Oxygen-17 nuclear magnetic resonance and oxygen exchange in aqueous solutions of telluric acid. (weizmann.ac.il)
PIPES [piperazine-N,N-bis(2-ethanesulfonic acid)] buffer was prepared. (tocris.com)

The pH of a solution is the negative logarithm of its hydronium ion concentration. (jove.com)
For example, a solution with 1 × 10 -5 M hydronium concentration has a pH of 5. (jove.com)
Solutions with a pH above 7 are called alkaline, as they have lower hydronium ion concentration. (jove.com)

In FPLC the mobile phase is an aqueous buffer solution. (wikipedia.org)
Based on the calculated pKa values, the mobile phase was buffered at pH 6. (europa.eu)

Working pH range in aqueous solution: 6.1 - 7.5. (tocris.com)

In either case the system allows the fraction of each buffer entering the column to be continuously varied. (wikipedia.org)

Typically the valve has three positions for loading the sample loop, for injecting the sample from the loop into the column, and for connecting the pumps directly to the waste line to wash them or change buffer solutions. (wikipedia.org)

To evaluate whether methylparaben and propylparaben, which present a similar chemical structure, increase fluoride uptake by demineralized dental enamel when present in buffered solutions. (bvsalud.org)

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0.015

0.093

Formaldehyde Solution2

Phosphate1

Reactivity1

Formalin2

Colorless1

Bovine1

Lysis2

Sodium hydroxide1

Methanol5

Kinetics1

Affinity1

Proteins4

0.051

Wash Buffer1

Bicarbonate2

Lysing Solution1

Methyl3

Phosphorus1

HPLC1

Hydrogen5

Methods1

Tris1

Shifts1

Resin4

Neutral2

Water6

Stable1

Injection1

Products1

Flow2

Applications2

Anhydrous2

Proportion1

Metal2

Acid3

Concentration3

Mobile phase2

Range1

System1

Typically1

Present1