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  • Protein
  • Identification of phosphorylation-dependent binding partners of aquaporin-2 using protein mass spectrometry. (nih.gov)
  • Protein kinase A phosphorylation is involved in regulated exocytosis of aquaporin-2 in transfected LLC-PK1 cells. (semanticscholar.org)
  • Aquaporin-2 is a water-transporting protein located in the principal cells of the kidney collecting duct . (ndif.org)
  • We demonstrate that members of several signaling pathways are activated by lithium treatment, including the PKB/Akt-kinase and the mitogen-activated protein kinases (MAPK), such as extracellular regulated kinase (ERK), c-Jun NH(2)-terminal kinase (JNK), and p38. (jhu.edu)
  • We generated Arabidopsis transgenic plants expressing a TIP2;2-GFP fusion protein driven by its own promoter, and showed several differences in TIP2;2 behavior between wild type and the phyA mutant. (mdpi.com)
  • Fluorescence of TIP2;2-GFP protein in the endodermis of roots in the wild-type seedlings increased during dark adaptation, but not in the phyA mutant. (mdpi.com)
  • The amount of the TIP2;2-GFP protein in wild-type seedlings decreased rapidly under far-red light illumination, and a delay in reduction of TIP2;2-GFP was observed in the phyA mutant. (mdpi.com)
  • Aquaporin 2 (Ab-256) Antibody detects endogenous levels of total Aquaporin 2 protein. (genetex.com)
  • The purpose of this study was to examine protein expression of renal sodium chloride and urea transporters and aquaporins in hypothyroid rats (HT) with diminished urinary concentration as compared with euthyroid controls (CTL) and hypothyroid rats replaced with L-thyroxine (HT+T). Hypothyroidism was induced by aminotriazole administration. (asnjournals.org)
  • Protein abundance of renal AQP1 and urea transporters UTA 1 and UTA 2 did not differ significantly among study groups. (asnjournals.org)
  • Exogenous administration of 1-deamino-8- d -AVP produced an antidiuresis and expressed AQP-2 mRNA and AQP-2 protein in the renal medulla of the homozygous Brattleboro rats. (physiology.org)
  • Increases in AQP-2 mRNA expression and AQP-2 protein were evident in Long-Evans rats after 64 h of water deprivation, with a severity of dehydration almost equal to the 12-h dehydrated, homozygous Brattleboro rats. (physiology.org)
  • There are a few reports in homozygous Brattleboro rats showing that there is no change in AQP-2 protein in response to water deprivation and that 1-deamino-8- d -arginine vasopressin (dDAVP) treatment increased its expression ( 4 , 20 , 26 ). (physiology.org)
  • abundance
  • After 1 or 2 weeks of lithium treatment, we identified 6 and 74 proteins with altered abundance compared with controls, respectively. (jhu.edu)
  • medullary
  • Aquaporin-2 expression in primary cultured rat inner medullary collecting duct cells. (semanticscholar.org)
  • We show here that primary cultures of rat inner medullary collecting duct (IMCD) cells retain AQP-2 expression for at least 6 days when grown with dibutyryl cAMP (DBcAMP) supplementation. (semanticscholar.org)
  • Twelve hours of water deprivation produced severe dehydration in the homozygous Brattleboro rats, such that urinary osmolality increased from 200 to 649 mosmol/kgH 2 O. However, no increase in AQP-2 mRNA expression was observed after this dehydration, and the medullary tissue content and urinary excretion of AQP-2 also remained unchanged. (physiology.org)
  • endogenous
  • The expression of AQP-2 mRNA is increased by exogenous and endogenous AVP in various pathophysiological conditions ( 1 , 7 , 18 , 30 ). (physiology.org)
  • rats
  • However, in the last 2 wk of study, HT+T rats also received daily intraperitoneal injection of L-thyroxine (Sigma) 50 μg/kg. (asnjournals.org)
  • Potassium
  • Activation of the countercurrent concentrating mechanism, which is initiated by increased sodium-potassium-2 chloride (Na-K-2Cl) co-transporter in the water impermeable ascending limb, creates the osmotic driving force for passive water reabsorption across the collecting duct. (asnjournals.org)
  • novel
  • In addition, a novel slow reversible fluorescence recovery (t(12) approximately 2 s) was characterized whose recovery was independent of spot size and not affected by fixation. (nih.gov)