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  • AQP1
  • This study aimed to obtain the complete cDNA coding sequences of aqp1 and aqp3 from the gills of Protopterus annectens , and to determine their branchial mRNA and protein expression levels during the induction, maintenance and arousal phases of aestivation. (frontiersin.org)
  • Dendrogramic analyses of the deduced Aqp1 and Aqp3 amino acid sequences of P. annectens revealed their close relationships with those of Latimeria chalumnae and tetrapods. (frontiersin.org)
  • During the induction phase, there were significant decreases in the transcript levels of aqp1 and aqp3 in the gills of P. annectens , but the branchial Aqp1 and Aqp3 protein abundance remained unchanged. (frontiersin.org)
  • As changes in transcription might precede changes in translation, this could be regarded as an adaptive response to decrease the protein abundance of Aqp1 and Aqp3 in the subsequent maintenance phase of aestivation. (frontiersin.org)
  • As expected, the branchial transcript levels and protein abundance of aqp1 /Aqp1 and aqp3 /Aqp3 were significantly down-regulated during the maintenance phase, probably attributable to the shutdown of branchial functions and the cessation of volume regulation of branchial epithelial cells. (frontiersin.org)
  • Indeed, the protein abundance of Aqp1 recovered partially, with complete recovery of mRNA expression level and protein abundance of Aqp3, in the gills of P. annectens after 3 days of arousal. (frontiersin.org)
  • Significant differences in the AQP1 , AQP5 and AQP9 genes expression between studied groups appeared only in the separated theca and granulosa cells. (jafs.com.pl)
  • In addition to AQP2, AQP3 and AQP4 are involved in the described AVP pathway, and AQP1 controls water reabsorption in the proximal tubule, in the descending thin limbs of Henle, and in the descending vasa recta. (pnas.org)
  • Pancreatic ductal HCO 3 - and fluid secretion were studied on pancreatic ducts isolated from wild-type (WT) and AQP1 knock out (KO) mice using microfluorometry and videomicroscopy, respectively. (frontiersin.org)
  • In the mice, the presence of AQP1 was detected throughout the whole plasma membrane of the ductal cells and its expression highly depends on the presence of CFTR Cl - channel. (frontiersin.org)
  • In contrast, the expression of AQP1 is mainly localized to the apical membrane of ductal cells in the human pancreas. (frontiersin.org)
  • Bile acid treatment dose- and time-dependently decreased mRNA and protein expression of AQP1 and reduced expression of this channel was also demonstrated in patients suffering from acute and chronic pancreatitis. (frontiersin.org)
  • HCO 3 - and fluid secretion significantly decreased in AQP1 KO versus WT mice and the absence of AQP1 also worsened the severity of pancreatitis. (frontiersin.org)
  • Our results suggest that AQP1 plays an essential role in pancreatic ductal fluid and HCO 3 - secretion and decreased expression of the channel alters fluid secretion which probably contribute to increased susceptibility of the pancreas to inflammation. (frontiersin.org)
  • gill
  • Immunolocalisation of aquaporin 3 in the gill and the gastrointestinal tract of the European eel Anguilla anguilla (L. (nii.ac.jp)
  • In teleost gill, AQP3 is expressed in 'chloride' cells, and in some species, in other epithelial cell types, where it may have a number of different functions including the prevention of dehydration. (antibodybeyond.com)
  • AQP-3 may play an important functional role in osmoregulation the teleostean gill but is unlikely to be responsible for the increases in intestinal water absorption that occur following SW acclimation. (antibodybeyond.com)
  • cDNA
  • The iScript advanced cDNA synthesis kit for RT-qPCR is a simple, fast, and sensitive first-strand cDNA synthesis kit for gene expression analysis by reverse-transcription qPCR. (bio-rad.com)
  • Molecular
  • Our data show that HSEE contains compounds capable of stimulating expression of biomarkers relevant to skin regeneration and hydration thereby counteracting molecular pathways leading to skin damage and aging. (hindawi.com)
  • Predicted molecular weight: 32 kDa.Can be blocked with Aquaporin 3 peptide (ab80550) . (abcam.com)
  • To elucidate the molecular mechanisms underling hydration during oocyte maturation, we characterized the structure of Japanese eel ( Anguilla japonica ) novel-water selective aquaporin 1 (AQP1b) that thought to be involved in oocyte hydration. (biomedcentral.com)
  • We've found that HDAC3 normally inhibits expression of AQP3 and we think we can use this knowledge to treat patients with psoriasis," said Dr. Vivek Choudhary, molecular biologist and physiologist in the Department of Physiology at the Medical College of Georgia at Augusta University. (medicaldialogues.in)
  • membranes
  • An intact stratum corneum and barrier, normal natural moisturizing factor and hyaluronic acid levels, normal Aquaporin-3 (AQP3) expression (localized at the basal lateral membranes of collecting duct cells in the kidney), and balanced sebum secretion are qualities of the skin that fall in the middle of the oily-dry spectrum. (gnpublication.org)
  • Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. (bio-rad.com)
  • cell membrane
  • Inside skin cells, phospholipase D - an enzyme that converts fats or lipids in the external protective cell membrane into cell signals - and AQP3 interact. (medicaldialogues.in)
  • The Bollag lab and others also had found that AQP3, which is present in psoriasis, appears rather immature and out of place, largely inside the cell cytoplasm instead of on the protective, outer cell membrane. (medicaldialogues.in)
  • hydration
  • Moreover, HSEE increased the expression of genes involved in skin hydration and homeostasis. (hindawi.com)
  • In the Japanese eel [ 5 ], although possible contribution of aquaporin on oocyte hydration is suggested, no direct evidence have not been available until today. (biomedcentral.com)
  • Mice lacking AQP3 have dry skin and reduced SC hydration. (antibodybeyond.com)
  • AQP3 knockout mice also further clarified AQP3's role in skin hydration, elasticity and wound healing and that it is glycerin - rather than water - that is most key to these healthy functions. (medicaldialogues.in)
  • genome
  • The Saccharomyces cerevisiae genome database contains two ORFs with homology to aquaporins, AQY1 and AQY2. (jhu.edu)
  • mice
  • In LXRβ −/− mice the expression of AVP was markedly decreased in the magnocellular neurons as well as in urine collected over a 24-h period. (pnas.org)
  • The persistent high urine volume after AVP administration was traced to a reduction in aquaporin-1 expression in the kidney of LXRβ −/− mice. (pnas.org)
  • Regulation
  • Regulation of the immunoexpression of aquaporin 9 by ovarian hormones in the rat oviductal epithelium. (jafs.com.pl)
  • Genes involved in cell volume regulation, nucleosome maintenance, ion transport, energetics, mitochondrion function, transcriptional regulation and apoptosis showed population- and salinity-dependent patterns of expression during acclimation. (biologists.org)
  • membrane
  • Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA). (abcam.com)
  • epithelium
  • these observations imply that the expression of c-kit in the colonic epithelium is involved in the proliferation and permeability of the colonic epithelium. (nih.gov)
  • regulates
  • Phospho-?Np63a regulates AQP3, ALOX12B, CASP14 and CLDN1 expression through transcription and microRNA modulation. (abcam.com)
  • These results provide insights into how P. annectens regulates branchial Aqp expression to cope with desiccation and rehydration during different phases of aestivation. (frontiersin.org)
  • inhibitors
  • They have shown that HDAC3 inhibitors are particularly adept at increasing expression of aquaporin-3, or AQP3, a channel that transports glycerin, a natural alcohol and water attractor, which helps skin look better and aids healthy production and maturation of high-turnover skin cells. (medicaldialogues.in)
  • They also found that p53, a known, natural tumor suppressor that also supports cell differentiation, helps the HDAC inhibitors enable more AQP3 and ultimately more glycerin, Choudhary said. (medicaldialogues.in)
  • They also used several other HDAC inhibitors and found the ones that suppressed HDAC3 were also most effective at increasing AQP3. (medicaldialogues.in)
  • kidney
  • 10. The isolated nephrospheroid of claim 9, characterized by enhanced expression of at least one polypeptide selected from the group consisting of sal1, pax2, six2 and WT1 as compared to said adult kidney cells grown under adherent conditions. (patentsencyclopedia.com)
  • 11. The isolated nephrospheroid of claim 9, characterized by enhanced expression of each of sal1, pax2, six2 and WT1 as compared to said adult kidney cells grown under adherent conditions. (patentsencyclopedia.com)
  • genes
  • Specifically, aquaporin 3 and filaggrin genes were enhanced by 20 and 58%, respectively. (hindawi.com)
  • The selected pathways were used to design panels of real-time PCR primers tailored for the top-ranked genes for differential gene expression analysis. (bio-rad.com)
  • Reference genes are used in relative gene expression analysis to normalize for variation in the amount of input messenger RNA (mRNA) among samples. (bio-rad.com)
  • To ensure accurate quantitation, it is important to include one or more reference genes exhibiting constant expression levels under the experimental conditions. (bio-rad.com)