• Unused nucleotides and ATP degrade to apyrase, allowing the reaction to start again with another nucleotide. (news-medical.net)
  • Unincorporated nucleotides and ATP are degraded by the apyrase, and the reaction can restart with another nucleotide. (wikidoc.org)
  • The DNA fragments are incubated with DNA polymerase, ATP sulfurylase, and apyrase enzymes, and adenosine 5' phosphosulfate and luciferin substrates. (news-medical.net)
  • ssDNA template is hybridized to a sequencing primer and incubated with the enzymes DNA polymerase , ATP sulfurylase , luciferase and apyrase , and with the substrates adenosine 5´ phosphosulfate (APS) and luciferin . (wikidoc.org)
  • Of these, enzyme-linked immunosorbent assay (ELISA) is used as a primary screening method and this is usually followed by western blotting (WB) and polymerase chain reaction (PCR) methods as confirmatory tests. (afpm.org.my)
  • ENTPD5 is similar to E-type nucleotidases (NTPases)/ecto-ATPase/apyrases. (wikipedia.org)
  • Ectonucleoside triphosphate diphosphohydrolase 5 is an enzyme that in humans is encoded by the ENTPD5 gene. (wikipedia.org)
  • Apyrases hydrolyze nucleoside triphosphates and diphosphates and are found in all eukaryotes and a few prokaryotes. (mpg.de)
  • A group of enzymes that catalyze the hydrolysis of diphosphate bonds in compounds such as nucleoside di- and tri-phosphates, and sulfonyl-containing anhydrides such as adenylylsulfate. (bvsalud.org)
  • In both THP-1 cells and newly isolated human being monocytes, addition from the ATP degrading enzyme apyrase (0.4?U?ml?1) to cell supernatants ahead of LPS activation didn't significantly inhibit the LPS-induced IL-1 launch. (bios-mep.info)
  • To be able to research the participation of ATP and P2X7 receptors in the LPS-induced IL-1 discharge in individual monocytes, OxATP, PPADS and KN-62, aswell as the ATP degrading enzyme, apyrase, had been tested because of their capability to inhibit LPS-induced IL-1 discharge in both THP-1 cell series and in newly isolated adherent individual monocytes. (bios-mep.info)
  • Furthermore apyrase didn't considerably alter LPS-induced launch of IL-1 in either the THP-1 cells or the adherent human being monocytes. (bios-mep.info)
  • Luciferase assays exposed that certainly ATP is usually released by monocytes activated with the various PAMPs or DAMPs (Fig. 4and 0.001) or KN-62 and apyrase ( 0.01). (conferencedequebec.org)
  • An inward current (Ix) was identified and this current was blocked by the P2 receptor antagonist, suramin, and inhibited by the ATP-degrading enzyme, apyrase. (ucl.ac.uk)
  • Certainly, antagonists from the P2X7 receptor (P2X7R), or treatment with apyrase, prevent IL-1 and IL-18 maturation and secretion brought about by the various stimuli. (conferencedequebec.org)
  • Silencing of the apyrase gene family with RNA interference constructs under the control of the constitutive 35S promoter led to a strong decrease in apyrase activity to below 10% of the wild-type level. (mpg.de)
  • Histone deacetylase 7 is an enzyme that in humans is encoded by the HDAC7 gene. (hdacassay.com)
  • It has also been used as a component of modified Tryptose phosphate/phenolphthalein/methyl green (M-TPMG) agar to detect enteroinvasive Escherichia coli (EIEC) apyrase activity. (sigmaaldrich.com)
  • In the treatment arm, an ATP hydrolyzing enzyme, apyrase, was applied directly to the site immediately after injury. (usuhs.edu)
  • DNA microarrays revealed that decreased expression of apyrase leads to increased levels of transcripts coding for cell wall proteins involved in growth and genes involved in energy transfer and starch synthesis. (mpg.de)
  • 001). Necrosis was less extensive in the apyrase group when compared with the burn group at 24 hours and 5 days. (usuhs.edu)
  • Apyrases are enzymes that catalyze the hydrolysis of nucleotide diphosphates and triphosphates in a calcium or magnesium-dependent manner. (nih.gov)
  • A calcium-activated enzyme that catalyzes the hydrolysis of ATP to yield AMP and orthophosphate. (nih.gov)
  • 13. Stage-specific expression of P2Y receptors, ecto-apyrase, and ecto-5'-nucleotidase in myeloid leukocytes. (nih.gov)
  • These antibodies can be measured by enzyme-linked immuno sorbent assays (ELISAs) using salivary gland homogenates (SGHs) as the source of antigens and serve as a markers for exposure to biting sand flies. (meganhardenfitness.com)
  • Enzymes that catalyze the transfer of glucose from a nucleoside diphosphate glucose to an acceptor molecule which is frequently another carbohydrate. (lookformedical.com)
  • An enzyme that catalyzes the synthesis of fructose-6-phosphate plus GLUTAMINE from GLUTAMATE plus glucosamine-6-phosphate. (lookformedical.com)
  • Inhibition of ALS enzyme will affect synthesis of the branched-chain amino acids (Val, Leu and Ile) and eventually result in the death of plants. (biomedcentral.com)
  • Recombinant apyrase rPorSP15, yellow-related protein rPorSP24, ParSP25-like protein rPorSP65, D7-related protein rPorSP67, and antigen 5-related protein rPorSP76 were tested using ELISA with sera of domestic animals from L. donovani foci in Ethiopia where Ph. (meganhardenfitness.com)
  • The principle of pyrosequencing was first described in 1993 [1] by, Bertil Pettersson, Mathias Uhlen and Pål Nyren by combining the solid phase sequencing method [2] using streptavidin coated magnetic beads with recombinant DNA polymerase lacking 3´to 5´exonuclease activity (proof-reading) and luminescence detection using the firefly luciferase enzyme. (wikipedia.org)
  • The pyrosequencing method is based on detecting the activity of DNA polymerase (a DNA synthesizing enzyme) with another chemoluminescent enzyme . (wikipedia.org)
  • Therefore, the electrostatic status of Y144, which is regulated by an unknown kinase/phosphatase system, may regulate AtGALT29A enzyme activity. (vtt.fi)
  • 7. Extracellular purine metabolism in blood vessels (Part II): Activity of ecto-enzymes in blood vessels of patients with abdominal aortic aneurysm. (nih.gov)
  • The third project employs nuclear magnetic resonance spectroscopy for experimental testing of molecules with inhibitory potential against L-aspartate-alpha-decarboxylase, an enzyme known for its critical role in the growth of microorganisms, such as Mycobacterium tuberculosis. (constructor.university)
  • Modulation of eATP levels with the ATP-degrading enzyme apyrase greatly reduced BLM-induced inflammatory cell recruitment, lung IL-1β, and tissue inhibitor of metalloproteinase (TIMP)-1 production, while administration of ATP-γS, a stable ATP derivative, enhanced inflammation. (pasteur.fr)