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Aptamers, NucleotideSELEX Aptamer TechniqueAptamers, PeptideRiboswitchNucleic Acid ConformationRNABiosensing TechniquesBase SequenceThermoanaerobacterOligonucleotidesRNA FoldingRegulatory Sequences, Ribonucleic AcidSurface Plasmon ResonanceStreptavidinG-QuadruplexesMolecular Sequence DataThrombinAntidotesLigandsOligoribonucleotidesBinding SitesModels, MolecularProtein BindingTenon CapsuleRosaniline DyesMolecular Probe TechniquesDNA, CatalyticElectrochemical TechniquesBase PairingGoldEntomoplasmataceaeRNA, BacterialNucleoside QNuclear Magnetic Resonance, BiomolecularS-AdenosylmethionineRNA, CatalyticNanotechnologyGuanineLimit of DetectionDrug Delivery SystemsAdrenergic beta-1 Receptor AgonistsMolecular MimicryDNA, Single-StrandedFluorescent DyesImmobilized ProteinsFactor IXaNucleic Acid DenaturationSolid-Phase Synthesis TechniquesMicrofluidic Analytical TechniquesCell Line, TumorFluorescenceCrystallography, X-RayMetals, Alkali2-AminopurineThermodynamicsDirected Molecular EvolutionNanoparticlesGene LibraryKineticsUntranslated RegionsOligodeoxyribonucleotidesDNAAdenineBisoprololFluorescence Resonance Energy TransferElectrophoresis, MicrochipDrug DesignRhodaminesMetal NanoparticlesArbovirus InfectionsSpectrometry, FluorescenceTetracyclineDNA PrimersBacillus subtilisAdrenergic beta-1 Receptor AntagonistsNF-kappa B p50 SubunitThrombocytopenia, Neonatal AlloimmuneNanostructures5' Untranslated RegionsFlavin MononucleotideHeparin Cofactor IIBinding, CompetitiveBlood CoagulationMolecular ProbesThiamine PyrophosphateTheophyllineCircular DichroismTenascinMagnetic Resonance SpectroscopyAdenosine MonophosphateArthus ReactionArbovirusesPeriosteumDrug CarriersImmunoglobulin EElectrodesHIV Long Terminal Repeat
High affinityOligonucleotidesLigandsNucleic acid aptamersProteinsSingle-strandedMoleculesBacterialSpecificityProteinTherapeuticVitroRNAsSELEXSelectivityDetectionAptasensorNanocomplexAntibodySiRNASelectiveLiving organismsTargetAssayFluorescenceDiagnosticsBoundActivateDetectCancer cellsModificationTechnologyDrugsSelectionUniqueCellPlatformRapidControlWorkProvide
High affinity8
  • Aptamers are engineered molecules that can bind to specific target compounds with high affinity and selectivity, making them valuable tools in various applications. (neoaptamers.com)
  • This aptamer has a universally high affinity for seven important SARS-CoV-2 variants, including Delta and Omicron. (chemistryviews.org)
  • For biosensing applications, aptamers are suitable high-affinity and cost-effective binding partners for their specific targets. (trentu.ca)
  • The review also outlines the vast applications of UBPs as novel information storage components as well as in creation of semi-synthetic organisms expressing non canonical amino acids, high affinity aptamer generation, PCR based diagnostics and sitespecific labelling of RNAs. (fortuneonline.org)
  • Using the SELEX methodology, the inventors generated multiple high affinity aptamers against GPVI from a pool of modified RNA sequences. (duke.edu)
  • Nucleic acid aptamers are "short single-stranded DNA- or RNA-based oligonucleotides that can selectively bind to small molecular ligands or protein targets with high affinity and specificity, when folded into their unique three-dimensional structure" (1). (ucf.edu)
  • The fluorescence of Cy3 greatly recovered after the addition of the exosomes which can specifically combine with the aptamer and release from the surface of Ti3C2 MXenes due to the high affinity between the aptamer and CD63 protein on exosome surface. (exosome-rna.com)
  • Aptamers are single-stranded DNA or RNA molecules that can bind biological targets from small molecules to proteins with high affinity and specificity. (skku-bmtl.org)
Oligonucleotides5
  • The team used DNA aptamers (oligonucleotides that bind to a specific target) selected from a DNA library to selectively bind to an intact, infectious virus. (chemistryviews.org)
  • The aim of this project is to identify therapeutic oligonucleotides (i.e. antisense and aptamers) that can selectively target these fusion proteins. (pasteur.fr)
  • Aptamers can be used as drug delivery systems for the treatment of GB and antisense oligonucleotides can silence essential genes involved in fusion protein expression. (pasteur.fr)
  • Aptamers are single-stranded oligonucleotides which can bind to target cells specifically by distinct tertiary structures even without knowledge of characteristic protein profiles on cell surface. (edu.hk)
  • In this section, we will discuss a few examples of using functionalized Au NPs as ultrasensitive tools for sensing DNA, aptamers, and oligonucleotides. (nanomaterialpowder.com)
Ligands5
  • Traditionally, the development of aptamers involves a process known as systematic evolution of ligands by exponential enrichment (SELEX). (neoaptamers.com)
  • Naturally, aptamers can be found in living organisms in the form of riboswitches, which function by binding with their ligands for subsequent regulation of specific gene expression [ 3 ] . (encyclopedia.pub)
  • Aptamers generated in vitro can be systematically isolated from a large pool of 1012-1015 synthetic oligo sequences using an iterative process called systematic evolution of ligands by exponential enrichment (SELEX). (encyclopedia.pub)
  • Ribonucleic acid ligands, or aptamers, are a class of drugs that are ideal for blocking GPVI. (duke.edu)
  • Previously, it was demonstrated that these aptamers can selectively deliver anticancer ligands," Dr. Cruz said, such as acridine orange derivatives, and showed improved selectivity towards cancer cell lines. (utexas.edu)
Nucleic acid aptamers2
  • The selectivity, low limit of detection, and cost-effectiveness make these nucleic acid aptamers optimal bio-sensors. (ucf.edu)
  • We establish a screening platform for nucleic acid aptamers through the screening aptamer for C-peptide of pre-proinsulin. (cgu.edu.tw)
Proteins4
  • Aptamers are being actively explored as molecular recognition elements for diagnostic assays, such as detecting specific proteins or nucleic acids in patient samples. (neoaptamers.com)
  • Due to their structure, they can selectively bind to proteins," researcher Justus Metternich said. (photonics.com)
  • The team used a pool of aptamers that bind to the wild-type spike protein and carried out aptamer selection experiments using five different spike proteins. (chemistryviews.org)
  • Beecher's research is looking to see if they can use an aptamer, that binds to proteins, that would be highly expressed in cancer. (missouri.edu)
Single-stranded4
  • Aptamers are short, single-stranded DNA or RNA molecules that can selectively bind to a specific target. (chemistryviews.org)
  • It utilizes aptamers, short single-stranded DNAs or RNAs, as pesticide recognition elements to integrate with various innovative biosensing technologies for specific and sensitive detection of pesticide residues. (encyclopedia.pub)
  • An aptamer requires either single-stranded DNA or RNA with a length of approximately 15-100 nucleotides to maintain its specific recognition and discrimination of the cognate target in a heterogeneous milieu [ 1 ] . (encyclopedia.pub)
  • With their tendency to form helices and single-stranded loops, aptamers have potential use in sensor, diagnostic, and therapeutic tools. (skku-bmtl.org)
Molecules10
  • Aptamers are a class of molecules that have garnered considerable attention in the field of molecular biology and diagnostics. (neoaptamers.com)
  • Aptamers are molecules that can be engineered to possess specific three-dimensional shapes, enabling them to selectively bind to target molecules. (neoaptamers.com)
  • Selectivity refers to the ability of an aptamer to distinguish its target molecule from other similar molecules. (neoaptamers.com)
  • Specificity goes beyond selectivity by necessitating that the aptamer exclusively binds to a single compound without any cross-reactivity with other molecules. (neoaptamers.com)
  • The primary challenge in developing aptamers with absolute specificity lies in the structural similarities that often exist between target and non-target molecules. (neoaptamers.com)
  • NeoVentures has over 20 years of experience in selecting and identifying aptamers that demonstrate unparalleled specificity, overcoming the intricate structural resemblances between target and non-target molecules. (neoaptamers.com)
  • Our team of dedicated scientists employs cutting-edge technologies and innovative methodologies to meticulously characterize the unique binding sites on target molecules, ensuring that the developed aptamers exhibit exceptional selectivity and accuracy. (neoaptamers.com)
  • The concept of polymer that can selectively recognize desired molecules have captured many attentions from scientific community over recent years. (biomedcentral.com)
  • Aptamer is a short-chain nucleic acids that can identify a variety of different molecules. (cgu.edu.tw)
  • and other molecules (pegaptanib, a DNA aptamer).7, 8, 9, 10, 11, 12 and 13 These agents have radically altered the management of neovascular AMD and have become the current inhibitors standard of care. (cxcrinhibitor.com)
Bacterial1
  • The antimicrobial wasp Polybia-MP1 peptide selectively kills bacterial cells! (biosyn.com)
Specificity9
  • The Specificity of Aptamers: Can They Selectively Bind to a Single Compound? (neoaptamers.com)
  • Continued research in aptamer design holds promise for improving their specificity in practical use cases. (neoaptamers.com)
  • While aptamers are recognized for their unique binding capabilities, one question that has intrigued researchers is whether aptamers can achieve the level of specificity required to selectively bind to a single compound. (neoaptamers.com)
  • In this blog, we explore the world of aptamers, their potential for selectivity, and the implications of this remarkable specificity for various applications. (neoaptamers.com)
  • Consequently, the resulting aptamers may lack the requisite selectivity and specificity for most applications. (neoaptamers.com)
  • As a result, the resulting aptamer sequences are guaranteed to exhibit a high degree of specificity and selectivity for the intended target. (neoaptamers.com)
  • Selectivity and specificity are two terms often used interchangeably, but they have distinct meanings in the context of aptamers. (neoaptamers.com)
  • Attaining absolute specificity can be demanding, as it requires an aptamer to exhibit both a high degree of selectivity and pinpoint accuracy in its binding. (neoaptamers.com)
  • Aptamers have shown promising ability for detection in the subnanomolar range of nucleic acid targets with specificity down to single nucleotide variations (2). (ucf.edu)
Protein4
  • To demonstrate the potential of the biosensor, the researchers designed sensors for the SARS CoV-2 spike protein and used aptamers to bind to the protein. (photonics.com)
  • Herein, LSPR aptamer sensors were fabricated for the detection of the SARS-CoV-2 protein. (trentu.ca)
  • The S1 aptamer selectively bound the S1 protein with high binding affinity. (trentu.ca)
  • Overall, LSPR has been demonstrated as a viable tool for measuring SARS-CoV-2 related aptamer-protein and protein-protein interactions, and this strategy may be applied to other viral or non-viral antigen targets. (trentu.ca)
Therapeutic3
  • The invention also provides nanosphere particle preparations that are useful for selectively delivering therapeutic, diagnostic, and/or other agents to cancer cells of subjects without eliciting a serotype-specific immunogenic response in the subjects. (justia.com)
  • However, NSPs described herein also may be used to selectively deliver other therapeutic and/or diagnostic agents as aspects of the invention are not limited in this respect. (justia.com)
  • Gold (Au) nanoparticles (AuNPs) exhibit a combination of physical, chemical, optical and electronic properties unique from other biomedical nanotechnologies and provide a highly multifunctional platform with which to image and diagnose diseases, to selectively deliver therapeutic agents to sensitize cells and tissues to treatment regimens, to monitor and guide surgical procedures, and to preferentially administer electromagnetic radiant to disease sites. (nanomaterialpowder.com)
Vitro3
  • The aptamers were tested in a platelet functionality assay (PFA-100) which measures time until platelet plug formation in vitro . (duke.edu)
  • Using cell-SELEX technology, the applicants obtained an aptamers L6 (named according to its sequence code) which could target osteoblasts but not hepatocytes and PBCs in vitro (Liang C et al. (edu.hk)
  • Further, osteoblast-selectivity of L6 aptamer did not alter after its linkage to LNPs-siRNA in vitro. (edu.hk)
RNAs2
  • The fact that RNAs from both motifs selectively bind glutamine supports this hypothesis, but detailed structural data is not yet available. (wikipedia.org)
  • Here we review some of these new discoveries and technologies, including long noncoding RNAs, microRNAs and RNA aptamers, and how they are being applied to prostate cancer research at the Brady Urological Institute. (blogspot.com)
SELEX1
  • During SELEX, a vast library of random nucleic acid sequences undergoes iterative rounds of selection, amplification, and enrichment to generate specific aptamer sequences. (neoaptamers.com)
Selectivity4
  • However, aptamers can still exhibit high selectivity, allowing them to differentiate between closely related compounds. (neoaptamers.com)
  • Aptamers find applications in medical diagnostics, therapeutics, and environmental monitoring, where their selectivity is crucial for accurate and effective results. (neoaptamers.com)
  • In order to improve the selectivity and efficacy of the aptamers, they were conjugated to gold nanoparticles, which are preferentially internalized by tumor cells. (utexas.edu)
  • Here, we propose to organize this aptamer into a split-aptamer system and determine the limit of detection and selectivity for a target gene in Mycobacterium tuberculosis . (ucf.edu)
Detection2
  • Herein, a unique Cy3 labeled CD63 aptamer (Cy3-CD63 aptamer)/Ti3C2 MXenes nanocomplex was constructed as a self-standard ratiometric fluorescence resonance energy transfer (FRET) nanoprobe for quantitative detection of exosomes. (exosome-rna.com)
  • Detection of DNA, aptamers, and oligonucleotide has received great attention in the past few years because it has important applications in medical research and diagnosis and food and drug industry monitoring. (nanomaterialpowder.com)
Aptasensor1
  • One of the most promising technologies is an aptamer-based biosensor or so-called aptasensor. (encyclopedia.pub)
Nanocomplex2
  • PEI nanocomplex synthesized with EpCAM aptamer (EpApt) and EpCAM siRNA (SiEp) showed 198 nm diameter sized particles by dynamic light scattering, spherical shaped particles, of 151 ± 11 nm size by TEM. (biomedcentral.com)
  • This nanocomplex exhibited decreased cell proliferation than the scrambled aptamer loaded nanocomplex in the EpCAM expressing cancer cells and may have potential for EpCAM targeting in vivo . (biomedcentral.com)
Antibody1
  • Moreover, we define the varied ideas of probe that may selectively detect the precise organic components when it comes to enzyme, antibody/nanobody, aptamer and ion-selective membrane. (superpcparts.com)
SiRNA3
  • Here, we report that employing EpCAM aptamer (EpApt) and EpCAM siRNA (SiEp) dual approach, for the targeted delivery of siRNA to EpCAM positive cancer cells, efficiently inhibits cancer cell proliferation. (biomedcentral.com)
  • The difference in size observed by DLS and TEM could be due to coating of aptamer and siRNA on PEI nanocore. (biomedcentral.com)
  • Thereafter, the applicants linked L6 aptamer to the surface of the PEG-modified LNPs encapsulating osteogenic siRNA, i.e. (edu.hk)
Selective1
  • Aptamers can be highly selective, binding to their intended target while ignoring closely related compounds. (neoaptamers.com)
Living organisms1
  • RNAi is a valuable research tool, both in cell culture and in living organisms, because synthetic dsRNA introduced into cells can selectively and robustly induce suppression of specific genes of interest. (skku-bmtl.org)
Target4
  • For aptamers to be highly specific, researchers must identify unique binding sites on the target molecule, which differentiates it from all other compounds. (neoaptamers.com)
  • The technology is based on nucleic acid binders (aptamers), which are evolved in a selection protocol and block several functional groups within a target molecule while allowing other functionalities not in contact with the aptamer to be selectively modified in a single reaction step. (rwth-aachen.de)
  • We're working to see if we can use that binding ability to selectively target chemotherapeutics, or anti-cancer drugs, to fight cancer cells," Beecher said. (missouri.edu)
  • In this study, a 3D-deformable dynamic microfilter was developed to selectively separate the target substance from a solution. (aip.org)
Assay1
  • An integrated colorimetric assay using this aptamer can detect five different variants, while other non-universal aptamers could only effectively detect one or two. (chemistryviews.org)
Fluorescence2
  • The Cy3-CD63 aptamer can be selectively adsorbed onto the Ti3C2 MXene nanosheets by hydrogen bond and metal chelate interaction between the aptamer and MXenes, and the fluorescence signal from Cy3-CD63 aptamer was quenched quickly owing to the FRET between the Cy3 and MXenes. (exosome-rna.com)
  • The fluorescence of CdTe QDs is selectively quenched by DA, whereas the fluorescence of CDs is insensitive to the analyte. (sharif.edu)
Diagnostics1
  • This extensive expertise positions NeoVentures at the forefront of aptamer development, facilitating the creation of highly specific molecular recognition tools for diverse applications in diagnostics, therapeutics, and beyond. (neoaptamers.com)
Bound1
  • When a virus is bound to the aptamers inside the nanopores, the ion-transport properties of the pores change measurably. (chemistryviews.org)
Activate1
  • FIRB 2013 - Development of drugs that selectively activate or inhibit the Na + / Ca2+ exchanger isoforms to improve learning and memory deficits. (unina.it)
Detect1
  • Yingfu Li, McMaster University, Hamilton, Canada, and colleagues have developed a universal DNA aptamer that can help to detect all major VoCs. (chemistryviews.org)
Cancer cells3
  • Flow cytometry analysis revealed that PEI-EpApt-SiEp has superior binding to cancer cells compared to EpApt or scramble aptamer (ScrApt) or PEI-ScrApt-SiEp. (biomedcentral.com)
  • These steps are repeated for several rounds to enrich the aptamer pool for cancer cells. (skku-bmtl.org)
  • The FLT3 inhibitor midostaurin selectively resensitizes ABCB1-overexpressing multidrug-resistant cancer cells to conventional chemotherapeutic agents. (cgu.edu.tw)
Modification1
  • This paper critically reviews the progression of pesticide aptasensors throughout the development process, including the selection, characterization and modification of aptamers, the conceptual frameworks of integrating aptamers and biosensors, the ASSURED (affordable, sensitive, specific, user-friendly, rapid and robust, equipment-free and deliverable to end users) criteria of different platforms and the future outlook. (encyclopedia.pub)
Technology1
  • Recently, Dr. Cruz and Dr. Ellington were interviewed by a representative from the UT Austin Portugal Program (a major partnership program in between the Portuguese Foundation for Science and Technology and UT) regarding their unique approach and explained how an aptamer known as AS1411 could be modified to better carry drugs to tumors. (utexas.edu)
Drugs1
  • Dan Passeri, MSc, JD, reviews a new class of synthetic biologic drugs engineered and designed to leverage the beneficial effect of IL-2 to selectively stimulate the proliferation and cytotoxic activity of disease-relevant T cells against cancer. (drug-dev.com)
Selection2
  • NeoVentures has pioneered and patented the Neomers approach , representing the next generation of aptamer selection. (neoaptamers.com)
  • Aptamers are generated using an unbiased selection process. (skku-bmtl.org)
Unique1
Cell1
  • PEI-EpApt-SiEp downregulated EpCAM and inhibited selectively the cell proliferation of MCF-7 and WERI-Rb1 cells. (biomedcentral.com)
Platform1
  • The platform showcases localized VEGF165 sequestration from the culture medium (offering spatial-control) and leverages aptamer-complementary sequence (CS) hybridization for triggering VEGF165 release (offering temporal-control), without non-specific leakage. (utwente.nl)
Rapid1
  • Rapid reversibility: Antidotes to the efficacious aptamers developed against GPVI allow for the fine-tuning of clinical response and can quickly abrogate the clinical effect if medically necessary. (duke.edu)
Control1
  • Furthermore, the inventors engineered inactivating aptamers that control GPVI aptamer activity, making it possible to fine-tune the clinical effect in patients. (duke.edu)
Work1
  • Previous work by our group has been done to optimize the signal of the dapoxyl-binding aptamer (DAP), which is a light-up aptamer. (ucf.edu)
Provide1
  • The discovery of this universal aptamer could provide a solution for testing for the constantly evolving SARS-CoV-2. (chemistryviews.org)