Activated clotting time (ACT) is a point-of-care coagulation test designed to monitor heparin therapy in the clinical situations in which intensive anticoagulation is required. (medscape.com)
The aPTT is commonly used to monitor heparin therapy, to target therapeutic anticoagulation, and as a part of a "coagulation panel" to evaluate a bleeding patient. (openanesthesia.org)
Tranexamic acid1
Despite the existence of a wide range of hemostatic drugs used in clinical practice, such as aprotinin, desmopressin, tranexamic acid, aminocaproic acid, their main drawback is the need for their local application. (nature.com)
Protease inhibitor1
Blood Sample Collection/Preparation At periodic intervals blood was collected (～0.5 ml) aseptically from Ivachtin the tail tip of anaesthetized rats (2 % isoflurane EZ Anesthesia Chamber with nose cone) (13 14 in sterile microtainer plasma separator tubes containing heparin and the protease inhibitor aprotinin (Sigma Chemicals St. Louis MO) to yield final blood concentrations of 10.74 units/ml and 0.016 units/ml respectively. (monossabios.com)
Anticoagulant6
ACT is intended to monitor anticoagulant effect of unfractionated heparin. (medscape.com)
Lupus anticoagulant (LA) also prolongs initial ACT values, and other strategies could be considered for heparin monitoring in patients with LA, such as doubling baseline ACT or in vitro heparin-ACT curves development. (medscape.com)
ACT is a point-of-care test used to monitor high-dose heparin as an anticoagulant during cardiac surgery (extracorporeal circulation), angioplasty, and hemodialysis. (unboundmedicine.com)
ACT is also used to monitor heparin or direct thrombin inhibitor in patients with lupus anticoagulant. (unboundmedicine.com)
Bivalirudin as an adjunctive anticoagulant to heparin in the treatment of heparin resistance during cardiopulmonary bypass-assisted cardiac surgery. (unboundmedicine.com)
Thirdly, to compare two different protocols to dose anticoagulant heparin in regard to thrombin building capacity. (5dok.org)
Bovine3
Aprotinin transcytosis across bovine brain capillary endothelial cell (BBCEC) monolayers is at least 10-fold greater than that of holo-transferrin. (aspetjournals.org)
Aprotinin is a basic proteinase inhibitor obtained from bovine organs, and is given intravenously to reduce peri-operative blood loss in open heart surgeries. (brainkart.com)
Aprotinin has been withdrawn from the Italian market based on concerns that it may transmit a bovine spongiform encephalopathy and/or a new variant Creutzfeldt-Jakob disease. (brainkart.com)
Protamine3
Normal activated clotting time (ACT) indicates that tested blood contains no heparin or that all heparin is inhibited by protamine (postoperative anticoagulation reversal). (medscape.com)
It is also used to determine the dose of protamine sulfate to reverse the heparin effect on completion of the procedure. (unboundmedicine.com)
Study III is a prospective trial, randomizing 60 elective CABG or valve replacement surgery patients to either anticoagulation with weight-based heparin dosing or using heparin and protamine titration with a bedside device. (5dok.org)
Anticoagulation1
Anticoagulation monitoring part 2: Unfractionated heparin and low-molecular-weight heparin. (medscape.com)
Fibrin3
Aprotinin is also combined with other components to be applied topically as a fibrin glue for wound haemostasis, suture support, and tissue adhesion or sealing. (brainkart.com)
In May 1998, the United States FDA approved the use of fibrin sealants containing aprotinin in multi-ingredient products. (brainkart.com)
Binding sites for heparin, fibrin/fibrinogen, collagen, von Willebrand factor and cell adhesion domains are localized on the molecule. (haemochrom.de)
Alternative coagulation tests (eg, anti-Xa assay) might be used for heparin monitoring in this situation. (medscape.com)
The assay can also be used for testing heparin analogues, such as Sodium Danaparoid (Orgaran®), or Pentasaccharide, ARIXTRA (Fondaparinux). (aniara.com)
Therapeutic1
During therapeutic use with aprotinin, the following have occurred infrequently: anaphylactic or anaphylactoid reactions which can range from mild to life-threatening symptoms and may not appear until the second or third dose. (brainkart.com)
Collagen1
4: Michopoulou A, Koliakou E, Terzopoulou Z, Rousselle P, Palamidi A, Anestakis D, Konstantinidou P, Roig-Rosello E, Demiri E, Bikiaris D. Benefit of coupling heparin to crosslinked collagen I/III scaffolds for human dermal fibroblast subpopulations' tissue growth J Biomed Mater Res A. 2022 Apr;110(4):797-811. (moleculardepot.com)
Perfusion1
We demonstrate here the transcytosis ability of aprotinin and peptides derived from Kunitz domains using an in vitro model of the BBB and in situ brain perfusion. (aspetjournals.org)
Cardiac surgery1
Coronary and arterial thrombosis have been reported in patients following the use of aprotinin during cardiac surgery, as well as, other types of surgery and/or disease processes. (brainkart.com)
Drugs1
Because different methodologies and a number of variables (eg, platelet count and function, hypothermia, hemodilution, and certain drugs like aprotinin) may affect the ACT, the ACT test is not yet standardized. (unboundmedicine.com)
Proteins1
Alignment of the amino acid sequence of aprotinin with the Kunitz domains of human proteins allowed the identification and design of a family of peptides, named Angiopeps. (aspetjournals.org)
Sucrose2
Crystal structures of aprotinin and its complex with sucrose octasulfate reveal multiple modes of interactions with implications for heparin binding. (expasy.org)
Sucrose permeability was unaffected by high concentrations of aprotinin, indicating that transcytosis of aprotinin was unrelated to changes in the BBCEC monolayer integrity. (aspetjournals.org)
Coagulation2
[ 2 ] Similar to partial thromboplastin time (PTT), ACT reflects time of clot formation via the intrinsic coagulation pathway by the addition of factor XII activators (eg, diatomaceous earth [Celite], kaolin, glass beads, ellagic acid) and increases linearly to relation to the heparin concentration. (medscape.com)
In controlled US trials with aprotinin, an incidence between 1% and 2% was reported for the following: throm-bocytopenia, leukocytosis, coagulation disorders (including disseminated intravascular coagulation). (brainkart.com)
Concentration1
Measuring the heparin concentration in patients' plasma for monitoring the therapy and adjusting drug dosage. (aniara.com)
Regulatory1
Recently, the relevant personnel of this research said that the decision of European and Canadian regulatory authorities to allow aprotinin to return to the market or the risk of endangering public life safety exists. (runquankang.cn)
Patients3
Alternatively, a more rapid detection of fibrinolytic activity, especially hyperfibrinolysis, is possible with thromboelastometry (TEM) in whole blood, even in patients on heparin. (wikipedia.org)
Although the value of the use of anticoagulants during and following administration of Activase has not been fully studied, heparin has been administered concomitantly for 24 hours or longer in more than 90% of patients. (wikidoc.org)
aspirin and/or dipyridamole have been given to patients receiving Alteplase during and/or following heparin treatment. (wikidoc.org)
Therapy2
Severe deficiency of contact activation factors (factor XII, prekallikrein, high-molecular-weight kininogen) does not cause increased bleeding risk during heparin therapy but does prolong the ACT and makes it impossible to use this test. (medscape.com)
Heparin therapy, direct thrombin inhibitor therapy, severe deficiency of clotting factors (except factors VII and XIII), functional platelet disorders. (unboundmedicine.com)
Chromogenic anti-Xa method for measuring homogeneously heparin (UFH) using the same calibration curve (kinetics method, one stage). (aniara.com)
Effects1
Several protease inhibitors, representing different classes of proteases (AEBSF, Pepstatin A, E-64, TPCK, PMSF and aprotinin), were tested for inhibitory effects on an RSV A2 infection of HEp-2 cells. (biomedcentral.com)
Capacity1
These peptides, and in particular Angiopep-2, exhibit higher transcytosis capacity and parenchyma accumulation than aprotinin. (aspetjournals.org)