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  • Polymerase Chain Re
  • DNA replication and transcription of DNA into RNA both rely upon nucleotide hybridization, as do molecular biology techniques including Southern blots and Northern blots, the polymerase chain reaction (PCR), and most approaches to DNA sequencing. (wikipedia.org)
  • A variety of different methods use hybridization to pinpoint the origin of a DNA sample, including the polymerase chain reaction (PCR). (wikipedia.org)
  • dsDNA
  • For example, all double-stranded nucleic acids - whether dsDNA, dsRNA or RNA:DNA hybrids - have specific 'melting temperatures', which depend mainly upon their specific guanine+cytosine content, but also upon whether they are DNA, RNA, or a mixture (RNA:RNA hybrids have the highest melting temperatures, followed by DNA:RNA hybrids, then dsDNA), and upon the ionic strength of solution. (uct.ac.za)
  • Thus
  • The global standard library of rRNA sequences is constantly becoming larger and continuously being updated, and thus the possibility of a random hybridization event between a specifically-designed probe (based on complete and current data from a range of test organisms) and an undesired/unknown target organism cannot be easily dismissed. (wikipedia.org)
  • As for the hybridization-ligation assay, the DLA is specific for the parent compound because the efficiency of ligation over a bulge loop is low, and thus the DLA detects the full-length analyte with both intact 5' and 3'-ends. (wikipedia.org)
  • Thus, small amounts of a nucleic acid can be detected and quantified without a reverse transcription step (in the case of RNA) and/or PCR. (wikipedia.org)
  • amino acids
  • The longest polypeptide of transmembrane protein 217 consists of 229 amino acids. (wikipedia.org)
  • Transmembrane protein 217 contains the domain of unknown function, DUF4534, between amino acids 11-171. (wikipedia.org)
  • These transmembrane domains consist primarily of uncharged amino acids in predicted alpha helices. (wikipedia.org)
  • The unmodified TEX36 consists of 186 amino acids and has a molecular weight of 21,545 daltons, with an isoelectric point of 9.1. (wikipedia.org)
  • Amino acids serine and lysine are highly represented in the protein at a higher frequency than observed in most proteins in vertebrates. (wikipedia.org)
  • approaches
  • Several approaches, such as microarray hybridization, have become extremely popular tools for specialists in biochemistry and biomedicine, while the potential of many other advantageous techniques seems to be underestimated. (indigo.ca)
  • whereas
  • Furthermore, DNA origami uses DNA hybridization interactions to realize a final structure, whereas SNAs and other forms of three-dimensional nucleic acids (structures templated with a triangular prism, rod, octahedra, or rhombic dodecadhedra-shaped nanoparticles) utilize the nanoparticle core to arrange the linear nucleic acid components into functional forms. (wikipedia.org)
  • electrophoresis
  • In particular, amino acid substitutions that change the electric charge of the enzyme (such as replacing aspartic acid with glutamic acid) are simple to identify by gel electrophoresis, and this forms the basis for the use of isozymes as molecular markers. (wikipedia.org)
  • concentrations
  • nylon membranes (Hybond-N, GeneScreen) bind all nucleic acids under a wide range of salt concentrations, and irreversible or covalent attachment can be achieved by UV irradiation for 5 min or less, or by treatment with 0.4M NaOH. (uct.ac.za)