• 2016). Application of locked nucleic acid-based probes in fluorescence in situ hybridization . (up.pt)
  • 2015). Towards fluorescence in vivo hybridization (FIVH) detection of H. pylori in gastric mucosa using advanced LNA probes . (up.pt)
  • DIG is used to label nucleic acid probes for applications such as in situ hybridization. (vectorlabs.com)
  • Furthermore, RNA probes hybridize much more efficiently to target molecules than DNA probes because there is no self-hybridization. (enzolifesciences.com)
  • Locked Nucleic Acid in PCR primers, qPCR probes, and other types of oligonucleotides is soluble in water and standard buffers as well as follows Watson-Crick base-pairing rules. (sigmaaldrich.com)
  • Locked Nucleic Acid Dual-Labeled Probes discriminate better than DNA Dual-Labeled Probes in SNP genotyping analysis9. (sigmaaldrich.com)
  • Locked Nucleic Acid PCR primers and qPCR probes are compatible with all real-time thermocyclers and end-point analytical detection instruments. (sigmaaldrich.com)
  • Detection of polynucleotide fragments using hybridization with probes labelled with digoxigenin. (bethlen-foundation.ro)
  • Nucleic acid dot blot hybridization with fragmented digoxygenin labelled probes in human papillomavirus DNA screening. (bethlen-foundation.ro)
  • Based on the knowledge and tools developed while identifying and characterizing new viruses and comparisons to previously-characterized viruses, new virus-specific and broad spectrum polyclonal and/or monoclonal antibody reagents, purification protocols, nucleic acid hybridization probes, PCR primers, isothermal amplification methods, and improved associated protocols will be developed. (usda.gov)
  • During the early 1990s, breakthroughs in cloning and sequencing of Norwalk virus and Southampton virus ( 15--18 ) led to the development of sensitive molecular assays (e.g., reverse transcription-polymerase chain reaction [RT-PCR]), nucleotide hybridization probes, and enzyme-linked immunosorbent assays (ELISA) that used baculovirus-expressed viral antigens ( 19--33 ). (cdc.gov)
  • The method is based upon the broadly-applicable principles of fluorescence-resonance energy transfer (FRET) and nucleic acid hybridization using fluorogenic oligonucleotide signaling probes originally reported for in tubo qRT-PCR applications, transfected into living cells. (genelink.com)
  • Detecting gamma-irradiated SARS-CoV-2 virions in an extraction buffer, the current amplified HCR lateral flow assay achieves a limit of detection of 200 copies/µL using nucleic acid probes to target the SARS-CoV-2 RNA genome. (caltech.edu)
  • By swapping out nucleic acid probes to target different pathogens, amplified HCR lateral flow assays offer a platform for adaptable and sensitive at-home testing for emergent diseases. (caltech.edu)
  • Cy3 plays a particularly important role in real-time PCR applications, being used as the reporter moiety in TaqMan probes (1), Scorpion primers (2) and Molecular Beacons (3). (genelink.com)
  • Cy3 can also be used to label DNA oligos for use as hybridization probes in other applications, such as Fluorescent In-Situ Hybridization (FISH). (genelink.com)
  • This Double Labeling of Oligonucleotide Probes for Fluorescence In Situ Hybridization (DOPE-FISH 0 strategy may provide an effective solution to the problem of low signal intensity, which is commonly observed when using corresponding singly-labeled FISH probes for microbe identification. (genelink.com)
  • In ECHO probes, a hybridization-dependent fluorescent nucleotide regulated by the H-aggregation of thiazole orange organic dyes (D 514 ) is incorporated into specific sequence contexts and serves as fluorescent detection readout for target nucleic acids. (ne.jp)
  • Multicolor detection and auxiliary functional modules have been built into ECHO probes to accommodate a broad range of biological applications. (ne.jp)
  • The target of ECHO probes is only the complementary single-stranded nucleic acid. (ne.jp)
  • If you have the data on the secondary structure of the target nucleic acid, it would give a hint for the design of probes. (ne.jp)
  • This value is about 50 to 100 times more sensitive than the level achieved with conventional fluorescencebased molecular beacons, and is 10 to 60 times more sensitive than previously reported for other lanthanidebased hybridization probes. (njit.edu)
  • These novel luminescent labels should significantly enhance the sensitivity of all type of nucleic acid hybridization probes, and could dramatically improve the detection limit of other biopolymers and small compounds that are used in a variety of biological applications. (njit.edu)
  • A method is described for visualizing three nucleic acid sequences simultaneously by in situ hybridization using a new blue immunofluorescent label, amino methyl coumarin acetic acid (AMCA), in combination with green and red fluorescing FITC and TRITC. (nih.gov)
  • Three-color in situ hybridization was applied to the study of numerical chromosome abnormalities as occur in human solid tumors. (nih.gov)
  • 2015). Erratum to: Mismatch discrimination in fluorescent in situ hybridization using different types of nucleic acids (Applied Microbiology and Biotechnology, 10.1007/s00253-015-6389-4) . (up.pt)
  • 2017). Detection and discrimination of biofilm populations using locked nucleic acid/2'-O-methyl-RNA fluorescence in situ hybridization (LNA/2'OMe-FISH) . (up.pt)
  • Peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) assay for specific detection of mycobacterium immunogenum and DNA-FISH assay for analysis of pseudomonads in metalworking fluid s and sputum. (cdc.gov)
  • Here, we report for the first time a species-specific peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) method for Mycobacterium immunogenum, a non-tuberculous Mycobacterium species prevalent in MWF and implicated in occupational lung disease hypersensitivity pneumonitis and pseudo-outbreaks. (cdc.gov)
  • The resultant DNA is suited for application in FISH, microarray gene expression profiling and other nucleic acid hybridization assays. (jenabioscience.com)
  • NC: Nitrocellulose membranes are widely used in biological research and diagnosis due to their ability to immobilize biomolecules such as proteins or nucleic acids for detection, such as Western blotting, dot blot assays, enzyme-linked immunosorbent assays (ELISA), and lateral flow assays. (szhhstech.com)
  • Our services range from hybridisation assays to Real-Time qPCR to detect your investigational new drug based on nucleic acids chemistry (RNA/DNA). (hum-molgen.org)
  • We also offer immunology assays to investigate immune response to nucleic acids therapeutics. (hum-molgen.org)
  • Solid phase hybridisation-based microplate assays have been uncovered to combine an excellent limit of detection (LOD) with outstanding assay performance at lower limit of quantification (LLOQ). (hum-molgen.org)
  • Accelerō Bioanalytics comprehensive service portfolio provides sandwich hybridisation assays of any kind: hybridisation assays for aptamers, CpG oligonucleotides, or antisense therapeutics, hybridisation-ligation assays, competitive hybridisation assays for antisense therapeutics, ligation assay/qPCR combinations to detect viral point mutations, branched DNA probe assays for transcript quantification, and many more. (hum-molgen.org)
  • 20fold more sensitive than for conventional sandwich hybridisation assays. (hum-molgen.org)
  • Accelerō Bioanalytics offers custom-made quantitative Real-Time RT-qPCR assays for your investigational new nucleic acid drug like siRNA, micro RNA, or RNA aptamers. (hum-molgen.org)
  • Other typical applications are: inflammation response monitoring (total and subclass IgG, IgE, IgA, IgM, cytokines, etc), assays for immunogenicity detection to your nucleic acid drugs or therapeutic monoclonal antibodies), complement activation monitoring, pathogen pharmacodynamics in antibiotics research (detection by ELISA or Real-Time qPCR), biomarker quantification as surrogate markers in preclinical or clinical drug developmen. (hum-molgen.org)
  • Further applications of this method in prenatal diagnosis for the detection of aneuploidy of the most frequently involved autosomes, as well as for the quantification of gene copy number and mRNA expression, are discussed. (nih.gov)
  • Nucleic acid hybridization techniques, exploited by colony hybridizations or polymerase chain reaction (PCR), apply a single detection method to a diversity of organisms. (cdc.gov)
  • 3EO Health startup uses the Wyss Institute's user-friendly diagnostic nucleic acid detection technology together with additional components to build a digital platform that may empower patients with self-testing capabilities and access to results, information, and clinical partners. (harvard.edu)
  • The detection of human papillomaviruses in histological preparations by using dot-blot hybridization. (bethlen-foundation.ro)
  • We then used the hybridization chain reaction mechanism to develop an adaptable and sensitive test for the detection of SARS-CoV-2. (caltech.edu)
  • Detection of 13 foodborne pathogens in aquatic products using visual chromogenic chips based on asymmetric multiplex polymerase chain reaction and nucleic acid hybridization. (cabi.org)
  • In this web page, recent advances in nucleic acid detection using exciton-controlled hybridization-sensitive fluorescent oligonucleotide (ECHO) probe technologies are introduced to you. (ne.jp)
  • Time-resolved acquisition of the luminescent signal that occurs upon hybridization of the probe to the target enabled the avoidance of short-lived background fluorescence, markedly enhancing the sensitivity of detection, which was less than 1 pM. (njit.edu)
  • Policy statement: automated real-time nucleic acid amplification technology for rapid and simultaneous detection of tuberculosis and rifampicin resistance: Xpert MTB/RIF system. (bvsalud.org)
  • FIND) has partnered with Cepheid, Inc. (Sunnyvale, CA) and the University of Medicine and Dentistry of New Jersey (UMDNJ, Newark, NY) to develop a TB-specific automated, cartridge-based nucleic amplification assay (Xpert MTB/RIF) based on the GeneXpert multi-disease platform, currently unique in its simplification of molecular testing with fully integrated and automated sample preparation, amplification and detection required for real-time polymerase chain reaction. (bvsalud.org)
  • The AF488 NT labeling mix contains specially optimized dUTP-XX-AF488 for incorporation into DNA by nick translation using DNA Polymerase I. The excellent stability and quantum yield of the fluorophore combined with a high incorporation rate of the dye-dUTP complex makes it the ideal choice for a broad range of fluorescence applications. (jenabioscience.com)
  • The laboratory section will provide students with hands-on experimentations in major techniques in molecular biology such as DNA and RNA isolation, protein purification, DNA and protein electrophoresis, nucleic acid hybridization and polymerase chain reaction. (aucegypt.edu)
  • Here, we enhance sensitivity by developing an amplified lateral flow assay that incorporates isothermal, enzyme-free signal amplification based on the mechanism of hybridization chain reaction (HCR). (caltech.edu)
  • Another crucial aspect of DNA nanotechnology is the hybridization of complementary chemical nucleic acids. (tatayoung.com)
  • The ratio of quantum yields between after and before addition of the complementary nucleic acid strands showed a strong relationship with the number of possible Watson-Crick base pairs formed within two bases from D 514 in the 'self-dimer' of the probe. (ne.jp)
  • Gillespie and Spiegelman (1) observed that single stranded DNA binds strongly to nitrocellulose membranes in a manner that minimises the two strands reassociating with each other, but allows the hybridisation to complementary RNA. (ddw-online.com)
  • These factors include the presence of solvent (formamide), hybridization temperature, length of hybridization, volume of hybridization solution, degree and method of agitation, use of blocking reagents, concentration and specific activity of the probe, use of molecular agents to increase the rate of nucleic acid reassociation, and the degree of stringency used during the washing of the membrane. (thomassci.com)
  • In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. (thomassci.com)
  • In addition, the improved hybridization of the PCR primer or qPCR probe to its target may increase the melting temperature (T m ) by up to 8 °C per Locked Nucleic Acid monomer substitution in medium salt conditions compared to native-state DNA oligonucleotides 4 ( Table 1 ). (sigmaaldrich.com)
  • Pink) mutant DNA analysis with Locked Nucleic Acid mutant probe (16mer with 3 Locked Nucleic Acid bases). (sigmaaldrich.com)
  • Purple) wild-type DNA with Locked Nucleic Acid mutant Probe (16mer with 3 Locked Nucleic Acid bases). (sigmaaldrich.com)
  • In the presence of target sequence, hybridization of the sequence-specific probe results in a fluorogenic conformational change. (genelink.com)
  • In contrast, strong emission is observed when the ECHO probe binds to its target nucleic acid, because the aggregate is dissolved and each dye independently intercalates into the nascent duplex structure. (ne.jp)
  • Food Microbiology Exam: Probe TechnologyProbe technology, also known as nucleic acid hybridization technology, has laid a good foundation for the application of DNA food microbiology techniques and ha. (elexbio.com)
  • A novel species-specific 14-bp PNA probe was designed for M. immunogenum based on its 16S rRNA gene sequence and was validated for specificity, by testing against a panel of other phylogenetically closely related rapidly growing mycobacteria and representative species of gram-positive, gram-negative, and acid fast organisms. (cdc.gov)
  • Nucleic acid labeling and hybridization on membranes have formed the basis for a range of experimental techniques involving understanding of gene expression, organization, etc. (teachntest.org)
  • In particular, these compounds can be used for labeling nucleic acids. (justia.com)
  • Incorporating Locked Nucleic Acid into oligonucleotides increases thermal duplex stability 2 and improves the specificity of oligonucleotide hybridization to target sequences. (sigmaaldrich.com)
  • The ability of oligonucleotides to discriminate between alleles via SNPs is greatly enhanced by the incorporation of Locked Nucleic Acid bases 6-8 ( Figure 2 ). (sigmaaldrich.com)
  • Shorter oligonucleotides incorporating Locked Nucleic Acid bases can be used at the same temperatures as longer native-state DNA oligonucleotides. (sigmaaldrich.com)
  • Due to Locked Nucleic Acid's improved hybridization characteristics with accompanied increase in T m , Locked Nucleic Acid oligonucleotides can be synthesized to be shorter, which overcomes certain design limitations that occur with native-state DNA oligonucleotides. (sigmaaldrich.com)
  • Specifically, Locked Nucleic Acid oligonucleotides can be designed to address traditionally problematic target sequences, such as AT- or GC-rich regions. (sigmaaldrich.com)
  • With Locked Nucleic Acid oligonucleotides, the selective placement of Locked Nucleic Acid bases facilitates the optimal design of highly-specific, shorter oligonucleotides that perform well, even at lengths of only 13 to 20 bases. (sigmaaldrich.com)
  • Also, the design of oligonucleotides for targeting difficult SNPs, such as the relatively stable G:T mismatch, is greatly facilitated by Locked Nucleic Acid. (sigmaaldrich.com)
  • The invention relates to design of short oligonucleotides and analogs thereof (such as, di-, and trinucleotide compounds) useful for various therapeutic applications. (justia.com)
  • The invention also relates to design modified oligonucleotides for therapeutic applications, by excluding nucleotide segments having off-target effects from the modified oligonucleotides. (justia.com)
  • We describe the development of a multiplex PCR that detects four categories of diarrheagenic E. coli and the application of the assay to human diarrheal stools and food in Mexico City. (cdc.gov)
  • This confers enhanced assay performance and an increased breadth of applications. (sigmaaldrich.com)
  • This increase in hybridization creates a significant broadening in the scope of assay conditions and allows for more successful multiplexing. (sigmaaldrich.com)
  • The mycobacterial PNA-FISH assay was successfully adapted to human sputum demonstrating its potential for clinical diagnostic applications in addition to industrial MWF monitoring, to assess MWF-associated exposures and pseudo-outbreaks. (cdc.gov)
  • 2001. Evaluation of benzene exposure in children living in Campania (Italy) by urinary trans,trans -muconic acid assay. (cdc.gov)
  • Blotting is the technique in which nucleic acids or proteins are immobilized onto a solid support generally nylon or nitrocellulose membranes. (teachntest.org)
  • Relative to proteins, complex nucleic acid-based systems can be designed more readily due to the countable nature of base-pairing interactions and readily available physical models. (caltech.edu)
  • The application of nucleic acid amplifications requires selecting appropriate oligonucleotide primers and optimizing conditions to maximize sensitivity and specificity. (cdc.gov)
  • Biotechnology is a rapidly evolving industry that holds immense potential for growth and innovation across a wide range of product applications and markets. (marketresearch.com)
  • PVDF: PVDF membranes are widely used in various filtration applications due to their excellent chemical compatibility and low protein binding properties in industries such as pharmaceuticals, biotechnology, food and beverage processing, electronics manufacturing, and water treatment. (szhhstech.com)
  • It introduces students to the experimental methods used in investigation and research in biotechnology applications. (aucegypt.edu)
  • A ccelerō Bioanalytics is a preferred partner in bioanalytical services for the nucleic acids biotechnology and pharmaceutical industry. (hum-molgen.org)
  • Another recently developed application of Real-Time qPCR uncovers an appealing area of increasing interest: the quantification of nucleic acid therapeutics in preclinical development. (hum-molgen.org)
  • Many factors contribute to the signal-to-noise ratio in nucleic acid hybridizations. (thomassci.com)
  • Another major application of DNA-based nanotechnology is the development and reprogramming of various types of sensors. (tatayoung.com)
  • NUPACK is a growing software suite for the analysis and design of nucleic acid structures, devices, and systems serving the needs of researchers in the fields of nucleic acid nanotechnology, molecular programming, synthetic biology, and across the life sciences. (caltech.edu)
  • It was used as negative control in DNA-DNA hybridization experiments using genomic DNA preparations of Vibrio parahaemolyticus and Vibrio alginolyticus. (thomassci.com)
  • Saline Sodium Citrate Buffer specifically developed for Northern and Southern transfer protocols and nucleic acid preparations. (aniara.com)
  • Early studies of DNA melting and reformation were carried out in aqueous solutions and yielded important information about the dependence of melting temperature (Tm) on the G+C composition and salt concentration, as well as information on the dependence of the rate of reassociation on the sequence complexity of the nucleic acid. (ddw-online.com)
  • The bold letters in the sequences denote Locked Nucleic Acid bases. (sigmaaldrich.com)
  • Dyskerin is composed of 514 amino acids and has a role in ribosomal RNA processing and telomere maintenance. (medscape.com)
  • The digoxigenin-labeled RNA transcripts produced by these reactions are suitable for a wide range of applications such as nucleic acid hybridization, sequencing, and genome analysis. (enzolifesciences.com)
  • Next generation sequencing (NGS) of nucleic acid extracts from plants infected with unknown viruses is expected to yield information about the genomes of previously uncharacterized viruses without any background information on what viruses might be infecting the plant. (usda.gov)
  • Food microbiology exams go beyond theoretical knowledge, often incorporating practical applications of food microbiology concepts. (elexbio.com)
  • Applications: Clarification of protein laden solutions. (aijirentech.com)
  • It is known for its high purity and low protein binding properties, making it suitable for applications requiring minimal interaction with filter substances. (szhhstech.com)
  • Application of VX-809, the CFTR corrector, restored CFTR and PC2 toward normal in the cilia, decreased the protein levels of septin 2, and drastically reduced septin 2 colocalization with CFTR. (bvsalud.org)
  • [ 6 ] Both technologies use hybridization of labeled nucleic acid transcripts to measure the gene expression. (medscape.com)
  • Such nucleic acid drugs can also comprise RNA transcripts or DNA plasmids for immune system modulation. (hum-molgen.org)
  • Developed at the Wyss Institute and licensed in 2022, their technology dictates cells' fates in a single step and has been validated for many applications. (harvard.edu)
  • The gap between fundamental technology and its practical application will be closed by the end of the year. (tatayoung.com)
  • This technology has allowed researchers to develop functionalized reprogrammable devices for different applications. (tatayoung.com)
  • PVDF (polyvinylidene fluoride) and NC (nitrocellulose) are two different types of filter membrane materials used in a variety of applications. (szhhstech.com)
  • These conjugates can be used as substrates for various kinds of polymerizing enzymes in the enzymatic synthesis of nucleic acids. (justia.com)
  • This thesis will first demonstrate the use of these nucleic acid molecular mechanisms in closed-loop genetic circuits and infectious disease testing using cgRNAs and HCR, respectively, then provide updated tools for the nucleic acid design community to exploit the programmable nature of nucleic acids. (caltech.edu)
  • The application of molecular biology to the answering of epidemiological questions. (bvsalud.org)
  • Electrophoresis - Definition, Principle, Working, Types, Applications Definition of Electrophoresis Electrophoresis is a technique used to separate molecules by size and charge. (teachntest.org)
  • Nucleic acid nanotechnologies have provided a platform where biologically relevant molecules can be engineered to perform programmable functions. (caltech.edu)
  • Blotting of nucleic acid is the central technique for hybridization studies. (teachntest.org)
  • Dot blotting and dot hybridisation (4) evolved out of the filter hybridisation technique and provided the basic concept for DNA arrays. (ddw-online.com)
  • The general principles and their application to virological diagnosis. (modelm.ro)
  • Table 1 Increasing the number of Locked Nucleic Acid bases in an oligonucleotide increases the T m . (sigmaaldrich.com)
  • Exceptional compatibility with acids, bases, and oxidizer. (aijirentech.com)
  • Major companies are focusing on developing a products with good sensitivity and performance for application such as pregnancy testing, COVID-19 testing, and cardiac marker testing. (marketsandmarkets.com)
  • The use of biological sensors to detect toxins as well as forensics and immunological tests are only a few of the potential applications. (tatayoung.com)
  • The presence of a single base mismatch has a greater destabilizing effect on the duplex formation between an Locked Nucleic Acid oligonucleotide and its target than with a native-state DNA oligonucleotide. (sigmaaldrich.com)
  • The introduction of solid supports for DNA hybridisation/reassociations greatly broadened the range of applications of nucleic acid hybridisations, and provided the basis for solid-based methods being used today. (ddw-online.com)
  • These Syringe Filters are ideal for filtering culture media and organic solvents in a wide range of applications. (aijirentech.com)
  • Deoxyribonucleic acid solution from calf thymus is suitable for use as a blocking agent in Southern hybridizations. (thomassci.com)
  • In order to build the devices, researchers have used self assembly and hybridization methods. (tatayoung.com)